Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,215,840	C→A	V213L (GTG→TTG)	yehW ←	glycine betaine ABC transporter membrane subunit YehW

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,215,840	0	C	A	100.0%	352.4 / NA	98	V213L (GTG→TTG)	yehW	glycine betaine ABC transporter membrane subunit YehW
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (43/55);  total (43/55)

TTAGGCGGAATTGGTGGCGTCATGCTCGCAGGTTATACCTTTATTTTGCGTGCTGGCTAAGCGCCTGCACCAGCCTTTCAAACAGGCGGTCTGCGATGATCGCCGCCAGTGCCACCAGTAACGCCCCCTGGATCACATACGCGGTATTAAATCCGCTAAGCCCGATGATGATGGGCGTACCCAGCGTGCTGGCCCCTACCGTTGAGGCGATCGTCGCCGTACCAATGTTGATAATCACCGAAGTTCGCACGCCCGCCAGAATCAC  >  NC_000913/2215705‑2215969                                                                                                                                        |                                                                                                                                  ttAGGCGGAATTGGTGGCGTCATGCTCGCAGGTTATACCTTTATTTTGCGTGCTGGCTAAGCGCCTGCACCAGCCTTTCAAACAGGCGGTCTGCGATGATCGCCGCCAGTGCCACCAGTAACGCCCCCTGGATCAAATAc                                                                                                                               >  1:1929735/1‑140 (MQ=255) ttAGGCGGAATTGGTGGCGTCATGCTCGCAGGTTATACCTTTATTTTGCGTGCTGGCTAAGCGCCTGCACCAGCCTTTCAAACAGGCGGTCTGCGATGATCGCCGCCAGTGCCACCAGTAACGCCCCCTGGATCAAATAc                                                                                                                               <  2:720013/140‑1 (MQ=255)  tAGGCGGAATTGGTGGCGTCATGCTCGCAGGTTATACCTTTATTTTGCGTGCTGGCTAAGCGCCTGCACCAGCCTTTCAAACAGGCGGTCTGCGATGATCGCCGCCAGTGCCACCAGTAACGCCCCCTGGATCAAATAcg                                                                                                                              <  2:84670/140‑1 (MQ=255)  tAGGCGGAATTGGTGGCGTCATGCTCGCAGGTTATACCTTTATTTTGCGTGCTGGCTAAGCGCCTGCACCAGCCTTTCAAACAGGCGGTCTGCGATGATCGCCGCCAGTGCCACCAGTAACGCCCCCTGGATCAAATAcg                                                                                                                              <  1:2108456/140‑1 (MQ=255)  tAGGCGGAATTGGTGGCGTCATGCTCGCAGGTTATACCTTTATTTTGCGTGCTGGCTAAGCGCCTGCACCAGCCTTTCAAACAGGCGGTCTGCGATGATCGCCGCCAGTGCCACCAGTAACGCCCCCTGGATCAAATAcg                                                                                                                              <  1:1753129/140‑1 (MQ=255)   aGGCGGAATTGGTGGCGTCATGCTCGCAGGTTATACCTTTATTTTGCGTGCTGGCTAAGCGCCTGCACCAGCCTTTCAAACAGGCGGTCTGCGATGATCGCCGCCAGTGCCACCAGTAACGCCCCTTGGATCAAATAcgc                                                                                                                             <  2:1054264/140‑1 (MQ=255)   aGGCGGAATTGGTGGCGTCATGCTCGCAGGTTATACCTTTATTTTGCGTGCTGGCTAAGCGCCTGCACCAGCCTTTCAAACAGGCGGTCTGCGATGATCGCCGCCAGTGCCACCAGTAACGCCCCCTGGATCAAATAcgc                                                                                                                             <  2:1228372/140‑1 (MQ=255)   aGGCGGAATTGGTGGCGTCATGCTCGCAGGTTATACCTTTATTTTGCGTGCTGGCTAAGCGCCTGCACCAGCCTTTCAAACAGGCGGTCTGCGATGATCGCCGCCAGTGCCACCAGTAACGCCCCCTGGATCAAATAcgc                                                                                                                             <  1:2101962/140‑1 (MQ=255)   aGGCGGAATTGGTGGCGTCATGCTCGCAGGTTATACCTTTATTTTGCGTGCTGGCTAAGCGCCTGCACCAGCCTTTCAAACAGGCGGTCTGCGATGATCGCCGCCAGTGCCACCAGTAACGCCCCCTGGATCAAATAcgc                                                                                                                             <  2:52745/140‑1 (MQ=255)      cGGAATTGGTGGCGTCATGCTCGCAGGTTATACCTTTATTTTGCGTGCTGGCTAAGCGCCTGCACCAGCCTTTCAAACAGGCGGTCTGCGATGATCGCCGCCAGTGCCACCAGTAACGCCCCCTGGATCAAATACGCGGt                                                                                                                          <  1:127141/140‑1 (MQ=255)         aaTTGGTGGCGTCATGCTCGCAGGTTATACCTTTATTTTGCGTGCTGGCTAAGCGCCTGCACCAGCCTTTCAAACAGGCGGTCTGCGATGATCGCCGCCAGTGCCACCAGTAACGCCCCCTGGATCAAATACGCGGTAtt                                                                                                                       >  1:467185/1‑140 (MQ=255)            tggtggCGTCATGCTCGCAGGTTATACCTTTATTTTGCGTGCTGGCTAAGCGCCTGCACCAGCCTTTCAAACAGGCGGTCTGCGATGATCGCCGCCAGTGCCACCAGTAACGCCCCCTGGATCAAATACGCGGTATTaaa                                                                                                                    <  1:1476780/140‑1 (MQ=255)             ggtggCGTCATGCTCGCAGGTTATACCTTTATTTTGCGTGCTGGCTAAGCGCCTGCACCAGCCTTTCAAACAGGCGGTCTGCGATGATCGCCGCCAGTGCCACCAGTAACGCCCCCTGGATCAAATACGCGGTATTAAAt                                                                                                                   <  1:230714/140‑1 (MQ=255)                  cGTCATGCTCGCAGGTTATACCTTTATTTTGCGTGCTGGCTAAGCGCCTGCACCAGCCTTTCAAACAGGCGGTCTGCGATGATCGCCGCCAGTGCCACCAGTAACGCCCCCTGGATCAAATACGCGGTATTaaa                                                                                                                    >  1:827569/1‑134 (MQ=255)                  cGTCATGCTCGCAGGTTATACCTTTATTTTGCGTGCTGGCTAAGCGCCTGCACCAGCCTTTCAAACAGGCGGTCTGCGATGATCGCCGCCAGTGCCACCAGTAACGCCCCCTGGATCAAATACGCGGTATTaaa                                                                                                                    <  2:827569/134‑1 (MQ=255)                  cGTCATGCTCGCAGGTTATACCTTTATTTTGCGTGCTGGCTAAGCGCCTGCACCAGCCTTTCAAACAGGCGGTCTGCGATGATCGCCGCCAGTGCCACCAGTAACGCCCCCTGGATCAAATACGCGGTATTAAATCCGCt                                                                                                              <  2:432635/140‑1 (MQ=255)                     cATGCTCGCAGGTTATACCTTTATTTTGCGTGCTGGCTAAGCGCCTGCACCAGCCTTTCAAACAGGCGGTCTGCGATGATCGCCGCCAGTGCCACCAGTAACGCCCCCTGGATCAAATACGCGGTATTAAATCCGCTAAg                                                                                                           <  1:1403188/140‑1 (MQ=255)                     cATGCTCGCAGGTTATACCTTTATTTTGCGTGCTGGCTAAGCGCCTGCACCAGCCTTTCAAACAGGCGGTCTGCGATGATCGCCGCCAGTGCCACCAGTAACGCCCCCTGGATCAAATACGCGGTATTAAATCCGCTAAg                                                                                                           <  1:27632/140‑1 (MQ=255)                     cATGCTCGCAGGTTATACCTTTATTTTGCGTGCTGGCTAAGCGCCTGCACCAGCCTTTCAAACAGGCGGTCTGCGATGATCGCCGCCAGTGCCACCAGTAACGCCCCCTGGATCAAATACGCGGTATTAAATCCGCTAAg                                                                                                           <  2:968439/140‑1 (MQ=255)                         cTCGCAGGTTATACCTTTATTTTGCGTGCTGGCTAAGCGCCTGCACCAGCCTTTCAAACAGGCGGTCTGCGATGATCGCCGCCAGTGCCACCAGTAACGCCCCCTGGATCAAATACGCGGTATTAAATCCGCt                                                                                                              >  2:1040181/1‑133 (MQ=255)                         cTCGCAGGTTATACCTTTATTTTGCGTGCTGGCTAAGCGCCTGCACCAGCCTTTCAAACAGGCGGTCTGCGATGATCGCCGCCAGTGCCACCAGTAACGCCCCCTGGATCAAATACGCGGTATTAAATCCGCt                                                                                                              <  1:1040181/133‑1 (MQ=255)                         cTCGCAGGTTATACCTTTATTTTGCGTGCTGGCTAAGCGCCTGCACCAGCCTTTCAAACAGGCGGTCTGCGATGATCGCCGCCAGTGCCACCAGTAACGCCCCCTGGATCAAATACGCGGTATTAAATCCGCTAAGCCCg                                                                                                       <  2:1977760/140‑1 (MQ=255)                         cTCGCAGGTTATACCTTTATTTTGCGTGCTGGCTAAGCGCCTGCACCAGCCTTTCAAACAGGCGGTCTGCGATGATCGCCGCCAGTGCCACCAGTAACGCCCCCTGGATCAAATACGCGGTATTAAATCCGCTAAGCCCg                                                                                                       <  1:1811249/140‑1 (MQ=255)                          tCGCAGGTTATACCTTTATTTTGCGTGCTGGCTAAGCGCCTGCACCAGCCTTTCAAACAGGCGGTCTGCGATGATCGCCGCCAGTGCCACCAGTAACGCCCCCTGGATCAAATACGCGGTATTAAATCCGCTAAGCCCga                                                                                                      <  1:1096825/140‑1 (MQ=255)                                gTTATACCTTTATTTTGCGTGCTGGCTAAGCGCCTGCACCAGCCTTTCAAACAGGCGGTCTGCGATGATCGCCGCCAGTGCCACCAGTAACGCCCCCTGGATCAAATACGCGGTATTAAATCCGCTAAGCCGgaggatga                                                                                                >  2:1558877/1‑140 (MQ=255)                                gTTATACCTTTATTTTGCGTGCTGGCTAAGCGCCTGCACCAGCCTTTCAAACAGGCGGTCTGCGATGATCGCCGCCAGTGCCACCAGTAACGCCCCCTGGATCAAATACGCGGTATTAAATCCGCTAAGCCCgatgatga                                                                                                <  2:1758993/140‑1 (MQ=255)                                gTTATACCTTTATTTTGCGTGCTGGCTAAGCGCCTGCACCAGCCTTTCAAACAGGCGGTCTGCGATGATCGCCGCCAGTGCCACCAGTAACGCCCCCTGGATCAAATACGCGGTATTAAATCCGCTAAGCCCgatgatga                                                                                                <  1:2035105/140‑1 (MQ=255)                                gTTATACCTTTATTTTGCGTGCTGGCTAAGCGCCTGCACCAGCCTTTCAAACAGGCGGTCTGCGATGATCGCCGCCAGTGCCACCAGTAACACCCCCTGGATCAAATACGCGGTATTAAATCCGCTAAGCCCgatgatga                                                                                                <  1:2501458/140‑1 (MQ=255)                                gTTATACCTTTATTTTGCGTGCTGGCTAAGCGCCTGCACCAGCCTTTCAAACAGGCGGTCTGCGATGATCACCGCCAGTGCCACCAGTAACGCCCCCTGGATCAAATACGCGGTATTAAATCCGCTAAGCCCgatgatga                                                                                                <  1:756569/140‑1 (MQ=255)                                  tataCCTTTATTTTGCGTGCTGGCTAAGCGCCTGCACCAGCCTTTCAAACAGGCGGTCTGCGATGATCGCCGCCAGTGCCACCAGTAACGCCCCCTGGATCAAATACGCGGTATTAAATCCGCTAAGCCCGatgatgatg                                                                                              >  1:468315/1‑140 (MQ=255)                                       cTTTATTTTGCGTGCTGGCTAAGCGCCTGCACCAGCCTTTCAAACAGGCGGTCTGCGATGATCGCCGCCAGTGCCACCAGTAACGCCCCCTGGATCAAATACGCGGTATTAAATCCGCTAAGCCCGATGATGATGGGCGt                                                                                         <  1:1905449/140‑1 (MQ=255)                                       cTTTATTTTGCGTGCTGGCTAAGCGCCTGCACCAGCCTTTCAAACAGGCGGTCTGCGATGATCGCCGCCAGTGCCACCAGTAACGCCCCCTGGATCAAATACGCGGTATTAAATCCGCTAAGCCCGATGATGATGGGCGt                                                                                         <  2:1335063/140‑1 (MQ=255)                                          tATTTTGCGTGCTGGCTAAGCGCCTGCAGCAGCCTTTCAAACAGTCGGTCTGGGTCGATCGCCGCGAGCGTCACCAGTAACACCCCCTGGATCCAATACGTGGGATTAACTCCGCCAAGCCCGATGTGGATGGGCGTatc                                                                                      >  2:2067310/1‑138 (MQ=255)                                          tATTTTGCGTGCTGGCTAAGCGCCTGCACCAGCCTTTCAAACAGGCGGTCTGCGATGATCGCCGCCAGTGCCACCAGTAACGCCCCCTGGATCAAATACGCGGTATTAAATCCGCTAAGCCCgatgatga                                                                                                <  2:1760794/130‑1 (MQ=255)                                          tATTTTGCGTGCTGGCTAAGCGCCTGCACCAGCCTTTCAAACAGGCGGTCTGCGATGATCGCCGCCAGTGCCACCAGTAACGCCCCCTGGATCAAATACGCGGTATTAAATCCGCTAAGCCCgatgatga                                                                                                >  1:1760794/1‑130 (MQ=255)                                          tATTTTGCGTGCTGGCTAAGCGCCTGCACCAGCCTTTCAAACAGGCGGTCTGCGATGATCGCCGCCAGTGCCACCAGTAACGCCCCCTGGATCAAATACGCGGTATTAAATCCGCTAAGCCCg                                                                                                       <  2:1946495/123‑1 (MQ=255)                                          tATTTTGCGTGCTGGCTAAGCGCCTGCACCAGCCTTTCAAACAGGCGGTCTGCGATGATCGCCGCCAGTGCCACCAGTAACGCCCCCTGGATCAAATACGCGGTATTAAATCCGCTAAGCCCg                                                                                                       >  1:1946495/1‑123 (MQ=255)                                          tATTTTGCGTGCTGGCTAAGCGCCTGCACCAGCCTTTCAAACAGGCGGTCTGCGATGATCGCCGCCAGTGCCACCAGTAACGCCCCCTGGATCAAATACGCGGTATTAAATCCGCTAAGCCCGATGATGATGGGCGTAcc                                                                                      >  2:2237001/1‑140 (MQ=255)                                          tATTTTGCGTGCTGGCTAAGCGCCTGCACCAGCCTTTCAAACAGGCGGTCTGCGATGATCGCCGCCAGTGCCACCAGTAACGCCCCCTGGATCAAATACGCGGTATTAAATCCGCTAAGCCCGATGATGATGGGCGTAcc                                                                                      >  2:1857362/1‑140 (MQ=255)                                          tATTTTGCGTGCTGGCTAAGCGCCTGCACCAGCCTTTCAAACAGGCGGTCTGCGATGATCGCCGCCAGTGCCACCAGTAACGCCCCCTGGATCAAATACGCGGTATTAAATCCGCTAAGCCCGATGATGATGGGCGTAcc                                                                                      >  1:1250853/1‑140 (MQ=255)                                            ttttGCGTGCTGGCTAAGCGCCTGCACCAGCCTTTCAAACAGGCGGTCTGCGATGATCGCCGCCAGTGCCACCAGTAACGCCCCCTGGATCAAATACGCGGTATTAAATCCGCTAAGCCCGATGATGATGGGCGTACCCa                                                                                    >  1:2249379/1‑140 (MQ=255)                                              ttGCGTGCTGGCTAAGCGCCTGCACCAGCCTTTCAAACAGGCGGTCTGCGATGATCGCCGCCAGTGCCACCAGTAACGCCCCCTGGATCAAATACGCGGTATTAAATCCGCTAAGCCCGATGATGATGGGCGTACCCAGc                                                                                  >  1:1759324/1‑140 (MQ=255)                                               tGCGTGCTGGCTAAGCGCCTGCACCAGCCTTTCAAACAGGCGGTCTGCGATGATCGCCGCCAGTGCCACCAGTAACGCCCCCTGGATCAAATACGCGGTATTAAATCCGCTAAGCCCGATGATGATGGGCGTACCCAGCg                                                                                 >  2:1705551/1‑140 (MQ=255)                                                 cGTGCTGGCTAAGCGCCTGCACCAGCCTTTCAAACAGGCGGTCTGCGATGATCGCCGCCAGTGCCACCAGTAACGCCCCCTGGATCAAATACGCGGTATTAAATCCGCTAAGCCCGATGATGATGGGCGTACCCAGCGTg                                                                               >  2:601591/1‑140 (MQ=255)                                                 cGTGCTGGCTAAGCGCCTGCACCAGCCTTTCAAACAGGCGGTCTGCGATGATCGCCGCCAGTGCCACCAGTAACGCCCCCTGGATCAAATACGCGGTATTAAATCCGCTAAGCCCGATGATGATGGGCGTACCCAGCGTg                                                                               >  1:1397454/1‑140 (MQ=255)                                                   tGCTGGCTAAGCGCCTGCACCAGCCTTTCAAACAGGCGGTCTGCGATGATCGCCGCCAGTGCCACCAGTAACGCCCCCTGGATCAAATACGCGGTATTAAATCCGCTAAGCCCGATGATGATGGGCGTACCCAGCGTGCt                                                                             >  1:2240031/1‑140 (MQ=255)                                                   tGCTGGCTAAGCGCCTGCACCAGCCTTTCAAACAGGCGGTCTGCGATGATCGCCGCCAGTGCCACCAGTAACGCCCCCTGGATCAAATACGCGGTATTAAATCCGCTAAGCCCGATGATGATGGGCGTACCCAGCGTGCt                                                                             <  2:422489/140‑1 (MQ=255)                                                    gCTGGCTAAGCGCCTGCACCAGCCTTTCAAACAGGCGGTCTGCGATGATCGCCGCCAGTGCCACCAGTAACGCCCCCTGGATCAAATACGCGGTATTAAATCCGCTAAGCCCGATGATGATGGGCGTACCCAGCGTGCTg                                                                            <  2:965671/140‑1 (MQ=255)                                                    gCTGGCTAAGCGCCTGCACCAGCCTTTCAAACAGGCGGTCTGCGATGATCGCCGCCAGTGCCACCAGTAACGCCCCCTGGATCAAATACGCGGTATTAAATCCGCTAAGCCCGATGATGATGGGCGTACCCAGCGTGCTg                                                                            >  2:1902318/1‑140 (MQ=255)                                                      tGGCTAAGCGCCTGCACCAGCCTTTCAAACAGGCGGTCTGCGATGATCGCCGCCAGTGCCACCAGTAACGCCCCCTGGATCAAATACGCGGTATTAAATCCGCTAAGCCCGATGATGATGGGCGTACCCAGCGTGCTGGc                                                                          <  2:468315/140‑1 (MQ=255)                                                      tGGCTAAGCGCCTGCACCAGCCTTTCAAACAGGCGGTCTGCGATGATCGCCGCCAGTGCCACCAGTAACGCCCCCTGGATCAAATACGCGGTATTAAATCCGCTAAGCCCGATGATGATGGGCGTACCCAGCGTGCTGGc                                                                          <  1:1705551/140‑1 (MQ=255)                                                         cTAAGCGCCTGCACCAGCCTTTCAGACAGGCGGTCTGCGATGATCGCCGCCAGTGCCACCAGTAACGCCCCCTGGATCAAATACTCGGTATTAAATCCGCTAAGCCCGATGATGATGGGCGTACCCAGCGTGCTGGcccc                                                                       <  2:2360411/140‑1 (MQ=255)                                                         cTAAGCGCCTGCACCAGCCTTTCAAACAGGCGGTCTGCGATGATCGCCGCCAGTGCCACCAGTAACGCCCCCTGGATCAAATACGCGGTATTAAATCCGCTAAGCCCGATGATGATGGGCGTACCCAGCGTGCTGGcccc                                                                       <  2:2240031/140‑1 (MQ=255)                                                                  tGCACCAGCCTTTCAAACAGGCGGTCTGCGATGATCGCCGCCAGTGCCACCAGTAACGCCCCCTGGATCAAATACGCGGTATTAAATCCGCTAAGCCCGATGATGATGGGCGTACCCAGCGTGCTGGCCCCTACCGTTGa                                                              >  2:676191/1‑140 (MQ=255)                                                                  tGCACCAGCCTTTCAAACAGGCGGTCTGCGATGATCGCCGCCAGTGCCACCAGTAACGCCCCCTGGATCAAATACGCGGTATTAAATCCGCTAAGCCCGATGATGATGGGCGTACCCAGCGTGCTGGCCCCTACCGTTGa                                                              >  1:2225937/1‑140 (MQ=255)                                                                  tGCACCAGCCTTTCAAACAGGCGGTCTGCGATGATCGCCGCCAGTGCCACCAGTAACGCCCCCTGGATCAAATACGCGGTATTAAATCCGCTAAGCCCGATGATGATGGGCGTACCCAGCGTGCTGGCCCCTACCGTTGa                                                              >  2:2078470/1‑140 (MQ=255)                                                                  tGCACCAGCCTTTCAAACAGGCGGTCTGCGATGATCGCCGCCAGTGCCACCAGTAACGCCCCCTGGATCAAATACGCGGTATTAAATCCGCTAAGCCCGATGATGATGGGCGTACCCAGCGTGCTGGCCCCTACCGTTGa                                                              >  1:142617/1‑140 (MQ=255)                                                                  tGCACCAGCCTTTCAAACAGGCGGTCTGCGATGATCGCCGCCAGTGCCACCAGTAACGCCCCCTGGATCAAATACGCGGTATTAAATCCGCTAAGCCCGATGATGATGGGCGTACCCAGCGTGCTGGCCCCTACCGTTGa                                                              >  2:808372/1‑140 (MQ=255)                                                                  tGCACCAGCCTTTCAAACAGGCGGTCTGCGATGATCGCCGCCAGTGCCACCAGTAACGCCCCCTGGATCAAATACGCGGTATTAAATCCGCTAAGCCCGATGATGATGGGCGTACCCAGCGTGCTGGCCCCTACCGTTGa                                                              >  2:606853/1‑140 (MQ=255)                                                                        aGCCTTTCAAACAGGCGGTCTGCGATGATCGCCGCCAGTGCCACCAGTAACGCCCCCTGGATCAAATACGCGGTATTAAATCCGCTAAGCCCGATGATGATGGGCGTACCCAGCGTGCTGGCCCCTACCGTTGAGGCGAt                                                        <  1:1902318/140‑1 (MQ=255)                                                                         gCCTTTCAAACAGGCGGTCTGCGATGATCGCCGCCAGTGCCACCAGTAACGCCCCCTGGATCAAATACGCGGTATTAAATCCGCTAAGCCCGATGATGATGGGCGTACCCAGCGTGCTGGCCCCTACCGTTGAGGCGAtc                                                       >  2:962530/1‑140 (MQ=255)                                                                            tttCAAACAGGCGGTCTGCGATGATCGCCGCCAGTGCCACCAGTAACGCCCCC‑GGATCAAATACGCGGTATTAAATCCGCTAAGCCCGATGATGATGGGCGTACCCAGCGTGCTGGCCCCTACCGTTGAGGCGAtcgtcg                                                   <  2:1545107/140‑1 (MQ=255)                                                                             ttCAAACAGGCGGTCTGCGATGATCGCCGCCAGTGCCACCAGTAACGCCCCCTGGATCAAATACGCGGTATTAAATCCGCTAAGCCCGATGATGATGGGCGTACCCAGCGTGCTGGCCCCTACCGTTGAGGCGAtcgtcg                                                   >  1:2559464/1‑140 (MQ=255)                                                                             ttCAAACAGGCGGTCTGCGATGATCGCCGCCAGTGCCACCAGTAACGCCCCCTGGATCAAATACGCGGTATTAAATCCGCTAAGCCCGATGATGATGGGCGTACCCAGCGTGCTGGCCCCTACCGTTGAGGCGAtcgtcg                                                   >  2:742724/1‑140 (MQ=255)                                                                              tCAAACAGGCGGTCTGCGATGATCGCCGCCAGTGCCACCAGTAACGCCCCCTGGATCAAATACGCGGTATTAAATCCGCTAAGCCCGATGATGATGGGCGTACCCAGCGTGCTGGCCCCTACCGTTGAGGCGATCGTCGc                                                  >  1:267674/1‑140 (MQ=255)                                                                              tCAAACAGGCGGTCTGCGATGATCGCCGCCAGTGCCACCAGTAACGCCCCCTGGATCAAATACGCGGTATTAAATCCGCTAAGCCCGATGATGATGGGCGTACCCAGCGTGCTGGCCCCTACCGTTGAGGCGATCGTCGc                                                  <  1:1553572/140‑1 (MQ=255)                                                                                aaaCAGGCGGTCTGCGATGATCGCCGCCAGTGCCACCAGTAACGCCCCCTGGATCAAATACGCGGTATTAAATCCGCTAAGCCCGATGATGATGGGCGTACCCAGCGTGCTGGCCCCTACCGTTGAGGCGATCGTCGCCg                                                <  2:1665245/140‑1 (MQ=255)                                                                                 aaCAGGCGGTCTGCGATGATCGCCGCCAGTGCCACCAGTAACGCCCCCTGGATCAAATACGCGGTATTAAATCCGCTAAGCCCGATGATGATGGGCGTACCCAGCGTGCTGGCCCCTACCGTTGAGGCGATCGTCGCCGt                                               >  2:466011/1‑140 (MQ=255)                                                                                  aCAGGCGGTCTGCGATGATCGCCGCCAGTGCCACCAGTAACGCCCCCTGGATCAAATACGCGGTATTAAATCCGCTAAGCCCGATGATGATGGGCGTACCCAGCGTGCTGGCCCCTACCGTTGAGGCGATCGTCGCCGTa                                              <  2:467185/140‑1 (MQ=255)                                                                                  aCAGGCGGTCTGCGATGATCGCCGCCAGTGCCACCAGTAACGCCCCCTGGATCAAATACGCGGTATTAAATCCGCTAAGCCCGATGATGATGGGCGTACCCAGCGTGCTGGCCCCTACCGTTGAGGCGATCGTCGCCGTa                                              <  1:2237001/140‑1 (MQ=255)                                                                                  aCAGGCGGTCTGCGATGATCGCCGCCAGTGCCACCAGTAACGCCCCCTGGATCAAATACGCGGTATTAAATCCGCTAAGCCCGATGATGATGGGCGTACCCAGCGTGCTGGCCCCTACCGTTGAGGCGATCGTCGCCGTa                                              <  2:1250853/140‑1 (MQ=255)                                                                                  aCAGGCGGTCTGCGATGATCGCCGCCAGTGCCACCAGTAACGCCCCCTGGATCAAATACGCGGTATTAAATCCGCTAAGCCCGATGATGATGGGCGTACCCAGCGTGCTGGCCCCTACCGTTGAGGCGATCGTCGCCGTa                                              <  1:1934699/140‑1 (MQ=255)                                                                                   cAGGCGGTCTGCGATGATCGCCGCCAGTGCCACCAGTAACGCCCCCTGGATCAAATACGCGGTATTAAATCCGCTAAGCCCGATGATGATGGGCGTACCCAGCGTGCTGGCCCCTACCGTTGAGGCGATCGTCGCCGTAc                                             <  2:1759324/140‑1 (MQ=255)                                                                                    aGGCGGTCTGCGATGATCGCCGCCAGTGCCACCAGTAACGCCCCCTGGATCAAATACGCGGTATTAAATCCGCTAAGCCCGATGATGATGGGCGTACCCAGCGTGCTGGCCCCTACCGTTGAGGCGATCGTCGCCGTAcc                                            <  1:1028823/140‑1 (MQ=255)                                                                                    aGGCGGTCTGCGATGATCGCCGCCAGTGCCACCAGTAACGCCCCCTGGATCAAATACGCGGTATTAAATCCGCTAAGCCCGATGATGATGGGCGTACCCAGCGTGCTGGCCCCTACCGTTGAGGCGATCGTCGCCGTAcc                                            <  2:2249379/140‑1 (MQ=255)                                                                                    aGGCGGTCTGCGATGATCGCCGCCAGTGCCACCAGTAACGCCCCCTGGATCAAATACGCGGTATTAAATCCGCTAAGCCCGATGATGATGGGCGTACCCAGCGTGCTGGCCCCTACCGTTGAGGCGATCGTCGCCGTAcc                                            <  2:142617/140‑1 (MQ=255)                                                                                     ggCGGTCTGCGATGATCGCCGCCAGTGCCACCAGTAACGCCCCCTGGATCAAATACGCGGTATTAAATCCGCTAAGCCCGATGATGATGGGCGTACCCAGCGTGCTGGCCCCTACCGTTGAGGCGATCGTCGCCGTACCa                                           >  2:2202781/1‑140 (MQ=255)                                                                                     ggCGGTCTGCGATGATCGCCGCCAGTGCCACCAGTAACGCCCCCTGGATCAAATACGCGGTATTAAATCCGCTAAGCCCGATGATGATGGGCGTACCCAGCGTGCTGGCCCCTACCGTTGAGGCGATCGTCGCCGTACCa                                           <  1:601591/140‑1 (MQ=255)                                                                                     ggCGGTCTGCGATGATCGCCGCCAGTGCCACCAGTAACGCCCCCTGGATCAAATACGCGGTATTAAATCCGCTAAGCCCGATGATGATGGGCGTACCCAGCGTGCTGGCCCCTACCGTTGAGGCGATCGTCGCCGTACCa                                           >  1:2490606/1‑140 (MQ=255)                                                                                          tCTGCGATGATCGCCGCCAGTGCCACCAGTAACGCCCCCTGGATCAAATACGCGGTATTAAATCCGCTAAGCCCGATGATGATGGGCGTACCCAGCGTGCTGGCCCCTACCGTTGAGGCGATCGTCGCCGTACCAATGtt                                      >  2:587258/1‑140 (MQ=255)                                                                                             gCGATGATCGCCGCCAGTGCCACCAGTAACGCCCCCTGGATCAAATACGCGGTATTAAATCCGCTAAGCCCGATGATGATGGGCGTACCCAGCGTGCTGGCCCCTACCGTTGAGGCGATCGTCGCCGTACCAATGTTGAt                                   <  1:2067310/140‑1 (MQ=255)                                                                                             gCGATGATCGCCGCCAGTGCCACCAGTAACGCCCCCTGGATCAAATACGCGGTATTAAATCCGCTAAGCCCGATGATGATGGGCGTACCCAGCGTGCTGGCCCCTACCGTTGAGGCGATCGTCGCCGTACCAATGTTGAt                                   <  2:667968/140‑1 (MQ=255)                                                                                                  gatCGCCGCCAGTGCCACCAGTAACGCCCCCTGGATCAAATACGCGGTATTAAATCCGCTAAGCCCGATGATGATGGGCGTACCCAGCGTGCTGGCCCCTACCGTTGAGGCGATCGTCGCCGTACCaa                                          >  2:2563831/1‑128 (MQ=255)                                                                                                  gatCGCCGCCAGTGCCACCAGTAACGCCCCCTGGATCAAATACGCGGTATTAAATCCGCTAAGCCCGATGATGATGGGCGTACCCAGCGTGCTGGCCCCTACCGTTGAGGCGATCGTCGCCGTACCaa                                          <  1:2563831/128‑1 (MQ=255)                                                                                                    tCGCCGCCAGTGCCACCAGTAACGCCCCCTGGATCAAATACGCGGTATTAAATCCGCTAAGCCCGATGATGATGGGCGTACCCAGCGTGCTGGCCCCTACCGTTGAGGCGATCGTCGCCGTACCAATGTTGATAATCAcc                            <  2:828946/140‑1 (MQ=255)                                                                                                    tCGCCGCCAGTGCCACCAGTAACGCCCCCTGGATCAAATACGCGGTATTAAATCCGCTAAGCCCGATGATGATGGGCGTACCCAGCGTGCTGGCCCCTACCGTTGAGGCGATCGTCGCCGTACCAATGTTGATAATCAcc                            <  1:82830/140‑1 (MQ=255)                                                                                                        cgccAGTGCCACCAGTAACGCCCCCTGGATCAAATACGCGGTATTAAATCCGCTAAGCCCGATGATGATGGGCGTACCCAGCGTGCTGGCCCCTACCGTTGAGGCGATCGTCGCCGTACCAATGTTGATAATCACCGAAg                        >  1:1363095/1‑140 (MQ=255)                                                                                                             gTGCCACCAGTAACGCCCCCTGGATCAAATACGCGGTATTAAATCCGCTAAGCCCGATGATGATGGGCGTACCCAGCGTGCTGGCCCCTACCGTTGAGGCGATCGTCGCCGTACCAATGTTGATAATCACCGAAGTTCGc                   >  2:469262/1‑140 (MQ=255)                                                                                                              tGCCACCAGTAACGCCCCCTGGATCAAATACGCGGTATTAAATCCGCTAAGCCCGATGATGATGGGCGTACCCAGCGTGCTGGCCCCTACCGTTGAGGCGATCGTCGCCGTACCAATGTTGATAATCACCGAAGTTCGCa                  <  2:1929735/140‑1 (MQ=255)                                                                                                              tGCCACCAGTAACGCCCCCTGGATCAAATACGCGGTATTAAATCCGCTAAGCCCGATGATGATGGGCGTACCCAGCGTGCTGGCCCCTACCGTTGAGGCGATCGTCGCCGTACCAATGTTGATAATCACCGAAGTTCGCa                  >  1:422087/1‑140 (MQ=255)                                                                                                                 caccaGTAACGCCCCCTGGATCAAATACGCGGTATTAAATCCGCTAAGCCCGATGATGATGGGCGTACCCAGCGTGCTGGCCCCTACCGTTGAGGCGATCGTCGCCGTACCAATGTTGATAATCACCGAAGTTCGCAcgc               <  1:606853/140‑1 (MQ=255)                                                                                                                  accaGTAACGCCCCCTGGATCAAATACGCGGTATTAAATCCGCTAAGCCCGATGATGATGGGCGTACCCAGCGTGCTGGCCCCTACCGTTGAGGCGATCGTCGCCGTACCAATGTTGATAATCACCGAAGTTCGCAcgcc              >  1:284900/1‑140 (MQ=255)                                                                                                                       tAACGCCCCCTGGATCAAATACGCGGTATTAAATCCGCTAAGCCCGATGATGATGGGCGTACCCAGCGTGCTGGCCCCTACCGTTGAGGCGATCGTCGCCGTACCAATGTTGATAATCACCGAAGTTCGCACGTCCGCCa         >  1:812104/1‑140 (MQ=255)                                                                                                                       tAACGCCCCCTGGATCAAATACGCGGTATTAAATCCGCTAAGCCCGATGATGATGGGCGTACCCAGCGTGCTGGCCCCTACCGTTGAGGCGATCGTCGCCGTACCAATGTTGATAATCACCGAAGTTCGCACGCCCGCCa         >  1:2325349/1‑140 (MQ=255)                                                                                                                         aCGCCCCCTGGATCAAATACGCGGTATTAAATCCGCTAAGCCCGATGATGATGGGCGTACCCAGCGTGCTGGCCCCTACCGTTGAGGCGATCGTCGCCGTACCAATGTTGATAATCACCGAAGTTCGCACGCCCGCCAGa       <  1:1902165/140‑1 (MQ=255)                                                                                                                            cccccTGGATCAAATACGCGGTATTAAATCCGCTAAGCCCGATGATGATGGGCGTACCCAGCGTGCTGGCCCCTACCGTTGAGGCGATCGTCGCCGTACCAATGTTGATAATCACCGAAGTTCGCACGCCCGCCAGAATc    >  1:520899/1‑140 (MQ=255)                                                                                                                             ccccTGGATCAAATACGCGGTATTAAATCCGCTAAGCCCGATGATGATGGGCGTACCCAGCGTGCTGGCCCCTACCGTTGAGGCGATCGTCGCCGTACCAATGTTGATAATCACCGAAGTTCGCAcg                >  1:373918/1‑127 (MQ=255)                                                                                                                             ccccTGGATCAAATACGCGGTATTAAATCCGCTAAGCCCGATGATGATGGGCGTACCCAGCGTGCTGGCCCCTACCGTTGAGGCGATCGTCGCCGTACCAATGTTGATAATCACCGAAGTTCGCAcg                <  2:373918/127‑1 (MQ=255)                                                                                                                              cccTGGATCAAATACGCGGTATTAAATCCGCTAAGCCCGATGATGATGGGCGTACCCAGCGTGCTGGCCCCTACCGTTGAGGCGATCGTCGCCGTACCAATGTTGATAATCACCGAAGTTCGCACGCCCGCCAGAATCAc  >  2:2390261/1‑140 (MQ=255)                                                                                                                              cccTGGATCAAATACGCGGTATTAAATCCGCTAAGCCCGATGATGATGGGCGTACCCAGCGTGCTGGCCCCTACCGTTGAGGCGATCGTCGCCGTACCAATGTTGATAATCACCGAAGTTCGCACGCCCGCCAGAATCAc  >  2:1775956/1‑140 (MQ=255)                                                                                                                                        |                                                                                                                                  TTAGGCGGAATTGGTGGCGTCATGCTCGCAGGTTATACCTTTATTTTGCGTGCTGGCTAAGCGCCTGCACCAGCCTTTCAAACAGGCGGTCTGCGATGATCGCCGCCAGTGCCACCAGTAACGCCCCCTGGATCACATACGCGGTATTAAATCCGCTAAGCCCGATGATGATGGGCGTACCCAGCGTGCTGGCCCCTACCGTTGAGGCGATCGTCGCCGTACCAATGTTGATAATCACCGAAGTTCGCACGCCCGCCAGAATCAC  >  NC_000913/2215705‑2215969

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑