Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,857,685 G→T R198L (CGT→CTT)  mutS → DNA mismatch repair protein MutS

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,857,6850GT100.0% 420.6 / NA 115R198L (CGT→CTT) mutSDNA mismatch repair protein MutS
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (62/53);  total (62/53)

Only 100 of 116 total aligned reads displayed.

GGGCGTTTTCGCCTGAGCGAACCGGCTGACCGCGAAACGATGGCGGCAGAACTGCAACGCACTAATCCTGCGGAACTGCTGTATGCAGAAGATTTTGCTGAAATGTCGTTAATTGAAGGCCGTCGCGGCCTGCGCCGTCGCCCGCTGTGGGAGTTTGAAATCGACACCGCGCGCCAGCAGTTGAATCTGCAATTTGGGACCCGCGATCTGGTCGGTTTTGGCGTCGAGAACGCGCCGCGCGGACTTTGTGCTGCCGGTTGTCTGTTGCAGTA  >  NC_000913/2857549‑2857820
                                                                                                                                        |                                                                                                                                       
gggCGTTTTCGCCTGAGCGAACCGGCTGACCGCGAAACGATGGCGGCAGAACTGCAACGCACTAATCCTGCGGAACTGCTGTATGCAGAAGATTTTGCTGAAATGTCGTTAATTGAAGGCCGTCGCGGCCTGCGCCTTCg                                                                                                                                      >  2:691776/1‑140 (MQ=255)
 ggCGTTTTCGCCTGAGCGAACCGGCTGACCGCGAAACGATGGCGGCAGAACTGCAACGCACTAATCCTGCGGAACTGCTGTATGCAGAAGATTTTGCTGAAATGTCGTTAATTGAAGGCCGTCGCGGCCTGCGCCTTCGc                                                                                                                                     >  2:19898/1‑140 (MQ=255)
 ggCGTTTTCGCCTGAGCGAACCGGCTGACCGCGAAACGATGGCGGCAGAACTGCAACGCACTAATCCTGCGGAACTGCTGTATGCAGAAGATTTTGCTGAAATGTCGTTAATTGAAGGCCGTCGCGGCCTGCGCCTTCGc                                                                                                                                     >  2:1499355/1‑140 (MQ=255)
 ggCGTTTTCGCCTGAGCGAACCGGCTGACCGCGAAACGATGGCGGCAGAACTGCAACGCACTAATCCTGCGGAACTGCTGTATGCAGAAGATTTTGCTGAAATGTCGTTAATTGAAGGCCGTCGCGGCCTGCGCCTTCGc                                                                                                                                     >  1:352825/1‑140 (MQ=255)
  gCGTTTTCGCCTGAGCGAACCGGCTGACCGCGAAACGATGGCGGCAGAACTGCAACGCACTAATCCTGCGGAACTGCTGTATGCAGAAGATTTTGCTGAAATGTCGTTAATTGAAGGCCGTCGCGGCCTGCGCCTTCGcc                                                                                                                                    <  1:565063/140‑1 (MQ=255)
  gCGTTTTCGCCTGAGCGAACCGGCTGACCGCGAAACGATGGCGGCAGAACTGCAACGCACTAATCCTGCGGAACTGCTGTATGCAGAAGATTTTGCTGAAATGTCGTTAATTGAAGGCCGTCGCGGCCTGCGCCTTCGcc                                                                                                                                    <  2:2162966/140‑1 (MQ=255)
  gCGTTTTCGCCTGAGCGAACCGGCTGACCGCGAAACGATGGCGGCAGAACTGCAACGCACTAATCCTGCGGAACTGCTGTATGCAGAAGATTTTGCTGAAATGTCGTTAATTGAAGGCCGTCGCGGCCTGCGCCTTCGcc                                                                                                                                    <  2:573485/140‑1 (MQ=255)
    gTTTTCGCCTGAGCGAACCGGCTGACCGCGAAACGATGGCGGCAGAACTGCAACGCACTAATCCTGCGGAACTGCTGTATGCAGAAGATTTTGCTGAAATGTCGTTAATTGAAGGCCGTCGCGGCCTGCGCCTTCGCCCg                                                                                                                                  <  1:140914/140‑1 (MQ=255)
    gTTTTCGCCTGAGCGAACCGGCTGACCGCGAAACGATGGCGGCAGAACTGCAACGCACTAATCCTGCGGAACTGCTGTATGCAGAAGATTTTGCTGAAATGTCGTTAATTGAAGGCCGTCGCGGCCTGCGCCTTCGCCCg                                                                                                                                  <  2:278375/140‑1 (MQ=255)
      tttCGCCTGAGCGAACCGGCTGACCGCGAAACGATGGCGGCAGAACTGCAACGCACTAATCCTGCGGAACTGCTGTATGCAGAAGATTTTGCTGAAATGTCGTTAATTGAAGGCCGTCGCGGCCTGCGCCTTCGCCCGCt                                                                                                                                >  1:1906406/1‑140 (MQ=255)
       ttCGCCTGAGCGAACCGGCTGACCGCGAAACGATGGCGGCAGAACTGCAACGCACTAATCCTGCGGAACTGCTGTATGCAGAAGATTTTGCTGAAATGTCGTTAATTGAAGGCCGTCGCGGCCTGCGCCTTCGCCCGCtg                                                                                                                               <  2:1647241/140‑1 (MQ=255)
              gAGCGAACCGGCTGACCGCGAAACGATGGCGGCAGAACTGCAACGCACTAATCCTGCGGAACTGCTGTATGCAGAAGATTTTGCTGAAATGTCGTTAATTGAAGGCCGTCGCGGCCTGCGCCTTCGCCCGCTGTGGGAGt                                                                                                                        <  2:489451/140‑1 (MQ=255)
               aGCGAACCGGCTGACCGCGAAACGATGGCGGCAGAACTGCAACGCACTAATCCTGCGGAACTGCTGTATGCAGAAGATTTTGCTGAAATGTCGTTAATTGAAGGCCGTCGCGGCCTGCGCCTTCGCCCGCTGTGGGAgat                                                                                                                       >  2:370185/1‑138 (MQ=255)
               aGCGAACCGGCTGACCGCGAAACGATGGCGGCAGAACTGCAACGCACTAATCCTGCGGAACTGCTGTATGCAGAAGATTTTGCTGAAATGTCGTTAATTGAAGGCCGTCGCGGCCTGCGCCTTCGCCCGCTGTGGGAGtt                                                                                                                       >  2:2009844/1‑140 (MQ=255)
                gCGAACCGGCTGACCGCGAAACGATGGCGGCAGAACTGCAACGCACTAATCCTGCGGAACTGCTGTATGCAGAAGATTTTGCTGAAATGTCGTTAATTGAAGGCCGTCGCGGCCTGCGCCTTCGCCCGCTGTGGGAGttt                                                                                                                      <  2:623037/140‑1 (MQ=255)
                gCGAACCGGCTGACCGCGAAACGATGGCGGCAGAACTGCAACGCACTAATCCTGCGGAACTGCTGTATGCAGAAGATTTTGCTGAAATGTCGTTAATTGAAGGCCGTCGCGGCCTGCGCCTTCGCCCGCTGTGGGAGttt                                                                                                                      >  1:67815/1‑140 (MQ=255)
                gCGAACCGGCTGACCGCGAAACGATGGCGGCAGAACTGCAACGCACTAATCCTGCGGAACTGCTGTATGCAGAAGATTTTGCTGAAATGTCGTTAATTGAAGGCCGTCGCGGCCTGCGCCTTCGCCCGCTGTGGGAGttt                                                                                                                      >  2:1345492/1‑140 (MQ=255)
                    aCCGGCTGACCGCGAAACGATGGCGGCAGAACTGCAACGCACTAATCCTGCGGAACTGCTGTATGCAGAAGATTTTGCTGAAATGTCGTTAATTGAAGGCCGTCGCGGCCTGCGCCTTCGCCCGCTGTGGGAGTTTGaaa                                                                                                                  >  2:2306111/1‑140 (MQ=255)
                              cgcgAAACGATGGCGGCAGAACTGCAACGCACTAATCCTGCGGAACTGCTGTATGCAGAAGATTTTGCTGAAATGTCGTTAATTGAAGGCCGTCGCGGCCTGCGCCTTCGCCCGCTGTGGGAGTTTGAAATCGACACcgc                                                                                                        >  1:1397797/1‑140 (MQ=255)
                              cgcgAAACGATGGCGGCAGAACTGCAACGCACTAATCCTGCGGAACTGCTGTATGCAGAAGATTTTGCTGAAATGTCGTTAATTGAAGGCCGTCGCGGCCTGCGCCTTCGCCCGCTGTGGGAGTTTGAAATCGACACcgc                                                                                                        >  1:1896598/1‑140 (MQ=255)
                                cgAAACGATGGCGGCAGAACTGCAACGCACTAATCCTGCGGAACTGCTGTATGCAGAAGATTTTGCTGAAATGTCGTTAATTGAAGGCCGTCGCGGCCTGCGCCTTCGCCCGCTGTGGGAGTTTGAAATCGACACcgcgc                                                                                                      <  1:1058337/140‑1 (MQ=255)
                                cgAAACGATGGCGGCAGAACTGCAACGCACTAATCCTGCGGAACTGCTGTATGCAGAAGATTTTGCTGAAATGTCGTTAATTGAAGGCCGTCGCGGCCTGCGCCTTCGCCCGCTGTGGGAGTTTGAAATCGACACcgcgc                                                                                                      <  1:28835/140‑1 (MQ=255)
                                  aaaCGATGGCGGCAGAACTGCAACGCACTAATCCTGCGGAACTGCTGTATGCAGAAGATTTTGCTGAAATGTCGTTAATTGAAGGCCGTCGCGGCCTGCGCCTTCGCCCGCTGTGGGAGTTTGAAATCGACACCgcgcgc                                                                                                    <  2:1847304/140‑1 (MQ=255)
                                    aCGATGGCGGCAGAACTGCAACGCACTAATCCTGCGGAACTGCTGTATGCAGAAGATTTTGCTGAAATGTCGTTAATTGAAGGCCGTCGCGGCCTGCGCCTTCGCCCGCTGTGGGAGTTTGAAATCGACACCGCGCGCca                                                                                                  <  2:1906406/140‑1 (MQ=255)
                                    aCGATGGCGGCAGAACTGCAACGCACTAATCCTGCGGAACTGCTGTATGCAGAAGATTTTGCTGAAATGTCGTTAATTGAAGGCCGTCGCGGCCTGCGCCTTCGCCCGCTGTGGGAGTTTGAAATCGACACCGCGCGCca                                                                                                  <  2:120532/140‑1 (MQ=255)
                                    aCGATGGCGGCAGAACTGCAACGCACTAATCCTGCGGAACTGCTGTATGCAGAAGATTTTGCTGAAATGTCGTTAATTGAAGGCCGTCGCGGCCTGCGCCTTCGCCCGCTGTGGGAGTTTGAAATCGACACCGCGCGCca                                                                                                  <  1:1664992/140‑1 (MQ=255)
                                    aCGATGGCGGCAGAACTGCAACGCACTAATCCTGCGGAACTGCTGTATGCAGAAGATTTTGCTGAAATGTCGTTAATTGAAGGCCGTCGCGGCCTGCGCCTTCGCCCGCTGTGGGAGTTTGAAATCGACACCGCGCGCca                                                                                                  <  1:873689/140‑1 (MQ=255)
                                    aCGATGGCGGCAGAACTGCAACGCACTAATCCTGCGGAACTGCTGTATGCAGAAGATTTTGCTGAAATGTCGTTAATTGAAGGCCGTCGCGGCCTGCGCCTTCGCCCGCTGTGGGAGTTTGAAATCGACACCGCGCGCca                                                                                                  <  1:1536947/140‑1 (MQ=255)
                                        tGGCGGCAGAACTGCAACGCACTAATCCTGCGGAACTGCTGTATGCAGAAGATTTTGCTGAAATGTCGTTAATTGAAGGCCGTCGCGGCCTGCGCCTTCGCCCGCTGTGGGAGTTTGAAATCGACACCGCGCGCcagcag                                                                                              >  2:1180282/1‑140 (MQ=255)
                                          gcggcAGAACTGCAACGCACTAATCCTGCGGAACTGCTGTATGCAGAAGATTTTGCTGAAATGTCGTTAATTGAAGGCCGTCGCGGCCTGCGCCTTCGCCCGCTGTGGGAGTTTGAAATCGACACCGCGCGCCAGCAGtt                                                                                            <  2:114532/140‑1 (MQ=255)
                                                gAACTGCAACGCACTAATCCTGCGGAACTGCTGTATGCAGAAGATTTTGCTGAAATGTCGTTAATTGAAGGCCGTCGCGGCCTGCGCCTTCGCCCGCTGTGGGAGTTTGAAATCGACACCGCGCGCCAGCAGTTGAATCt                                                                                      <  2:2194173/140‑1 (MQ=255)
                                                 aaCTGCAACGCACTAATCCTGCGGAACTGCTGTATGCAGAAGATTTTGCTGAAATGTCGTTAATTGAAGGCCGTCGCGGCCTGCGCCTTCGCCCGCTGTGGGAGTTTGAAATCGACACCGCGCGCCAGCAGTTGAATCTg                                                                                     <  1:2009844/140‑1 (MQ=255)
                                                 aaCTGCAACGCACTAATCCTGCGGAACTGCTGTATGCAGAAGATTTTGCTGAAATGTCGTTAATTGAAGGCCGTCGCGGCCTGCGCCTTCGCCCGCTGTGGGAGTTTGAAATCGACACCGCGCGCCAGCAGTTGAATCTg                                                                                     <  1:1499355/140‑1 (MQ=255)
                                                      cAACGCACTAATCCTGCGGAACTGCTGTATGCAGGAGATTTTGCTGAAATGTCGTTAATTGAAGGCCGTCGCGGCCTGCGCCTTCGCCCGCTGTGGGAGTTTGAAATCGACACCGCGCGCCAGCAGTTGAATCTGCAAtt                                                                                >  1:971963/1‑140 (MQ=255)
                                                      cAACGCACTAATCCTGCGGAACTGCTGTATGCAGAAGATTTTGCTGAAATGTCGTTAATTGAAGGCCGTCGCGGCCTGCGCCTTCGCCCGCTGTGGGAGTTTGAAATCGACACCGCGCGCca                                                                                                  <  2:640294/122‑1 (MQ=255)
                                                      cAACGCACTAATCCTGCGGAACTGCTGTATGCAGAAGATTTTGCTGAAATGTCGTTAATTGAAGGCCGTCGCGGCCTGCGCCTTCGCCCGCTGTGGGAGTTTGAAATCGACACCGCGCGCca                                                                                                  >  1:640294/1‑122 (MQ=255)
                                                      cAACGCACTAATCCTGCGGAACTGCTGTATGCAGAAGATTTTGCTGAAATGTCGTTAATTGAAGGCCGTCGCGGCCTGCGCCTTCGCCCGCTGTGGGAGTTTGAAATCGACACCGCGCGCCAGCAGTTGAATCTGCAAtt                                                                                >  2:1117824/1‑140 (MQ=255)
                                                           cACTAATCCTGCGGAACTGCTGTATGCAGAAGATTTTGCTGAAATGTCGTTAATTGAAGGCCGTCGCGGCCTGCGCCTTCGCCCGCTGTGGGAGTTTGAAATCGACACCGCGCGCCAGCAGTTGAATCTGCAATTTgg                                                                             >  1:2553473/1‑138 (MQ=255)
                                                           cACTAATCCTGCGGAACTGCTGTATGCAGAAGATTTTGCTGAAATGTCGTTAATTGAAGGCCGTCGCGGCCTGCGCCTTCGCCCGCTGTGGGAGTTTGAAATCGACACCGCGCGCCAGCAGTTGAATCTGCAATTTgg                                                                             <  2:2553473/138‑1 (MQ=255)
                                                           cACTAATCCTGCGGAACTGCTGTATGCAGAAGATTTTGCTGAAATGTCGTTAATTGAAGGCCGTCGCGGCCTGCGCCTTCGCCCGCTGTGGGAGTTTGAAATCGACACCGCGCGCCAGCAGTTGAATCTGCAATTTGGGa                                                                           >  1:1489896/1‑140 (MQ=255)
                                                            aCTAATCCTGCGGAACTGCTGTATGCAGAAGATTTTGCTGAAATGTCGTTAATTGAAGGCCGTCGCGGCCTGCGCCTTCGCCCGCTGTGGGAGTTTGAAATCGACACCGCGCGCCAGCAGTTGAATCTGCAATTTGGGAc                                                                          <  1:2271032/140‑1 (MQ=255)
                                                              tAATCCTGCGGAACTGCTGTATGCAGAAGATTTTGCTGAAATGTCGTTAATTGAAGGCCGTCGCGGCCTGCGCCTTCGCCCGCTGTGGGAGTTTGAAATCGACACCGCGCGCCAGCAGTTGAATCTGCAATTTGGGAccc                                                                        >  2:749900/1‑140 (MQ=255)
                                                                  ccTGCGGAACTGCTGTATGCAGAAGATTTTGCTGAAATGTCGTTAATTGAAGGCCGTCGCGGCCTGCGCCTTCGCCCGCTGTGGGAGTTTGAAATCGACACCGCGCGCCAGCAGTTGAATCTGCAATTTGGGACCCGCGa                                                                    <  2:2236702/140‑1 (MQ=255)
                                                                     gCGGAACTGCTGTATGCAGAAGATTTTGCTGAAATGTCGTTAATTGAAGGCCGTCGCGGCCTGCGCCTTCGCCCGCTGTGGGAGTTTGAAATCGACACCGCGCGCCAGCAGTTGAATCTGCAATTTGGGACCCGCGATCt                                                                 >  1:1896343/1‑140 (MQ=255)
                                                                      cGGAACTGCTGTATGCAGAAGATTTTGCTGAAATGTCGTTAATTGAAGGCCGTCGCGGCCTGCGCCTTCGCCCGCTGTGGGAGTTTGAAATCGACACCGCGCGCCAGCAGTTGAATCTGCAATTTGGGACCCGCGATCTg                                                                >  1:1578475/1‑140 (MQ=255)
                                                                      cGGAACTGCTGTATGCAGAAGATTTTGCTGAAATGTCGTTAATTGAAGGCCGTCGCGGCCTGCGCCTTCGCCCGCTGTGGGAGTTTGAAATCGACACCGCGCGCCAGCAGTTGAATCTGCAATTTGGGACCCGCGATCTg                                                                >  1:547943/1‑140 (MQ=255)
                                                                        gAACTGCTGTATGCAGAAGATTTTGCTGAAATGTCGTTAATTGAAGGCCGTCGCGGCCTGCGCCTTCGCCCGCTGTGGGAGTTTGAAATCGACACCGCGCGCCAGCAGTTGAATCTGCAATTTGGGACCCGCGATCTGGt                                                              >  2:1483329/1‑140 (MQ=255)
                                                                          aCTGCTGTATGCAGAAGATTTTGCTGAAATGTCGTTAATTGAAGGCCGTCGCGGCCTGCGCCTTCGCCCGCTGTGGGAGTTTGAAATCGACACCGCGCGCCAGCAGTTGAATCTGCAATTTGGGACCCGCGATCTGGTCg                                                            >  1:561903/1‑140 (MQ=255)
                                                                          aCTGCTGTATGCAGAAGATTTTGCTGAAATGTCGTTAATTGAAGGCCGTCGCGGCCTGCGCCTTCGCCCGCTGTGGGAGTTTGAAATCGACACCGCGCGCCAGCAGTTGAATCTGCAATTTGGGACCCGCGATCTGGTCg                                                            >  1:548985/1‑140 (MQ=255)
                                                                          aCTGCTGTATGCAGAAGATTTTGCTGAAATGTCGTTAATTGAAGGCCGTCGCGGCCTGCGCCTTCGCCCGCTGTGGGAGTTTGAAATCGACACCGCGCGCCAGCAGTTGAATCTGCAATTTGGGACCCGCGATCTGGTCg                                                            >  1:213745/1‑140 (MQ=255)
                                                                                   tGCAGAAGATTTTGCTGAAATGTCGTTAATTGAAGGCCGTCGCGGCCTGCGCCTTCGCCCGCTGTGGGAGTTTGAAATCGACACCGCGCGCCAGCAGTTGAATCTGCAATTTGGGACCCGCGATCTGGTCGGTTTTGGCg                                                   >  2:2175540/1‑140 (MQ=255)
                                                                                   tGCAGAAGATTTTGCTGAAATGTCGTTAATTGAAGGCCGTCGCGGCCTGCGCCTTCGCCCGCTGTGGGAGTTTGAAATCGACACCGCGCGCCAGCAGTTGAATCTGCAATTTGGGACCCGCGATCTGGTCGGTTTTGGCg                                                   >  1:745112/1‑140 (MQ=255)
                                                                                      agaagaTTTTGCTGAAATGTCGTTAATTGAAGGCCGTCGCGGCCTGCGCCTTCGCCCGCTGTGGGAGTTTGAAATCGACACCGCGCGCCAGCAGTTGAATCTGCAATTTGGGACCCGCGATCTGGTCGGTTTTGGCGTCg                                                >  2:1379535/1‑140 (MQ=255)
                                                                                      agaagaTTTTGCTGAAATGTCGTTAATTGAAGGCCGTCGCGGCCTGCGCCTTCGCCCGCTGTGGGAGTTTGAAATCGACACCGCGCGCCAGCAGTTGAATCTGCAATTTGGGACCCGCGATCTGGTCGGTTTTGGCGTCg                                                >  1:1961293/1‑140 (MQ=255)
                                                                                        aagaTTTTGCTGAAATGTCGTTAATTGAAGGCCGTCGCGGCCTGCGCCTTCGCCCGCTGTGGGAGTTTGAAATCGACACCGCGCGCCAGCAGTTGAATCTGCAATTTGGGACCCGCGATCTGGTCGGTTTTGGCGTCgag                                              >  1:1090872/1‑140 (MQ=255)
                                                                                        aagaTTTTGCTGAAATGTCGTTAATTGAAGGCCGTCGCGGCCTGCGCCTTCGCCCGCTGTGGGAGTTTGAAATCGACACCGCGCGCCAGCAGTTGAATCTGCAATTTGGGACCCGCGATCTGGTCGGTTTTGGCGTCgag                                              >  2:201495/1‑140 (MQ=255)
                                                                                        aagaTTTTGCTGAAATGTCGTTAATTGAAGGCCGTCGCGGCCTGCGCCTTCGCCCGCTGTGGGAGTTTGAAATCGACACCGCGCGCCAGCAGTTGAATCTGCAATTTGGGACCCGCGATCTGGTCGGTTTTGGCGTCgag                                              >  1:402128/1‑140 (MQ=255)
                                                                                        aagaTTTTGCTGAAATGTCGTTAATTGAAGGCCGTCGCGGCCTGCGCCTTCGCCCGCTGTGGGAGTTTGAAATCGACACCGCGCGCCAGCAGTTGAATCTGCAATTTGGGACCCGCGATCTGGTCGGTTTTGGCGTCgag                                              >  2:2484491/1‑140 (MQ=255)
                                                                                        aagaTTTTGCTGAAATGTCGTTAATTGAAGGCCGTCGCGGCCTGCGCCTTCGCCCGCTGTGGGAGTTTGAAATCGACACCGCGCGCCAGCAGTTGAATCTGCAATTTGGGACCCGCGATCTGGTCGGTTTTGGCGTCgag                                              >  2:561679/1‑140 (MQ=255)
                                                                                         agaTTTTGCTGAAATGTCGTTAATTGAAGGCCGTCGCGGCCTGCGCCTTCGCCCGCTGTGGGAGTTTGAAATCGACACCGCGCGCCAGCAGTTGAATCTGCAATTTGGGACCCGCGATCTGGTCGGTTTTGGCGTCgaga                                             >  2:1800584/1‑140 (MQ=255)
                                                                                           aTTTTGCTGAAATGTCGTTAATTGAAGGCCGTCGCGGCCTGCGCCTTCGCCCGCTGTGGGAGTTTGAAATCGACACCGCGCGCCAGCAGTTGAATCTGCAATTTGGGACCCGCGATCTGGTCGGTTTTGGCGTCGAGAAc                                           >  1:1018697/1‑140 (MQ=255)
                                                                                             tttGCTGAAATGTCGTTAATTGAAGGCCGTCGCGGCCTGCGCCTTCGCCCGCTGTGGGAGTTTGAAATCGACACCGCGCGCCAGCAGTTGAATCTGCAATTTGGGACCCGCGATCTGGTCGGTTTTGGCGTCGAGAAcgc                                         >  1:277649/1‑140 (MQ=255)
                                                                                             tttGCTGAAATGTCGTTAATTGAAGGCCGTCGCGGCCTGCGCCTTCGCCCGCTGTGGGAGTTTGAAATCGACACCGCGCGCCAGCAGTTGAATCTGCAATTTGGGACCCGCGATCTGGTCGGTTTTGGCGTCGAGAAcgc                                         >  2:772038/1‑140 (MQ=255)
                                                                                             tttGCTGAAATGTCGTTAATTGAAGGCCGTCGCGGCCTGCGCCTTCGCCCGCTGTGGGAGTTTGAAATCGACACCGCGCGCCAGCAGTTGAATCTGCAATTTGGGACCCGCGATCTGGTCGGTTTTGGCGTCGAGAAcgc                                         >  2:2171660/1‑140 (MQ=255)
                                                                                             tttGCTGAAATGTCGTTAATTGAAGGCCGTCGCGGCCTGCGCCTTCGCCCGCTGTGGGAGTTTGAAATCGACACCGCGCGCCAGCAGTTGAATCTGCAATTTGGGACCCGCGATCTGGTCGGTTTTGGCGTCGAGAAcgc                                         >  2:407332/1‑140 (MQ=255)
                                                                                               tGCTGAAATGTCGTTAATTGAAGGCCGTCGCGGCCTGCGCCTTCGCCCGCTGTGGGAGTTTGAAATCGACACCGCGCGCCAGCAGTTGAATCTGCAATTTGGGACCCGCGATCTGGTCGGTTTTGGCGTCGAGAAcgcgc                                       >  2:1132778/1‑140 (MQ=255)
                                                                                                  tGAAATGTCGTTAATTGAAGGCCGTCGCGGCCTGCGCCTTCGCCCGCTGTGGGAGTTTGAAATCGACACCGCGCGCCAGCAGTTGAATCTGCAATTTGGGACCCGCGATCTGGTCGGTTTTGGCGTCGAGAAcgcgccgc                                    >  2:1503835/1‑140 (MQ=255)
                                                                                                  tGAAATGTCGTTAATTGAAGGCCGTCGCGGCCTGCGCCTTCGCCCGCTGTGGGAGTTTGAAATCGACACCGCGCGCCAGCAGTTGAATCTGCAATTTGGGACCCGCGATCTGGTCGGTTTTGGCGTCGAGAAcgcgccgc                                    >  2:178905/1‑140 (MQ=255)
                                                                                                   gAAATGTCGTTAATTGAAGGCCGTCGCGGCCTGCGCCTTCGCCCGCTGTGGGAGTTTGAAATCGACACCGCGCGCCAGCAGTTGAATCTGCAATTTGGGACCCGCGATCTGGTCGGTTTTGGCGTCGAGAAcgcgccgcg                                   <  2:971963/140‑1 (MQ=255)
                                                                                                   gAAATGTCGTTAATTGAAGGCCGTCGCGGCCTGCGCCTTCGCCCGCTGTGGGAGTTTGAAATCGACACCGCGCGCCAGCAGTTGAATCTGCAATTTGGGACCCGCGATCTGGTCGGTTTTGGCGTCGAGAAcgcgccgcg                                   <  1:1180282/140‑1 (MQ=255)
                                                                                                    aaaTGTCGTTAATTGAAGGCCGTCGCGGCCTGCGCCTTCGCCCGCTGTGGGAGTTTGAAATCGACACCGCGCGCCAGCAGTTGAATCTGCAATTTGGGACCCGCGATCTGGTCGGTTTTGGCGTCGAGAAcgcgccgcgc                                  >  2:1651485/1‑140 (MQ=255)
                                                                                                    aaaTGTCGTTAATTGAAGGCCGTCGCGGCCTGCGCCTTCGCCCGCTGTGGGAGTTTGAAATCGACACCGCGCGCCAGCAGTTGAATCTGCAATTTGGGACCCGCGATCTGGTCGGTTTTGGCGTCGAGAAcgcgccgcgc                                  <  1:2175540/140‑1 (MQ=255)
                                                                                                             tAATTGAAGGCCGTCGCGGCCTGCGCCTTCGCCCGCTGTGGGAGTTTGAAATCGACACCGCGCGCCAGCAGTTGAATCTGCAATTTGGGACCCGCGATCTGGTCGGTTTTGGCGTCGAGAACGCGCCGCGCGGACTTtgt                         <  1:1800584/140‑1 (MQ=255)
                                                                                                               aTTGAAGGCCGTCGCGGCCTGCGCCTTCGCCCGCTGTGGGAGTTTGAAATCGACACCGCGCGCCAGCAGTTGAATCTGCAATTTGGGACCCGCGATCTGGTCGGTTTTGGCGTCGAGAACGCGCCGCGCGGACTTTGtgc                       <  1:1331754/140‑1 (MQ=255)
                                                                                                               aTTGAAGGCCGTCGCGGCCTGCGCCTTCGCCCGCTGTGGGAGTTTGAAATCGACACCGCGCGCCAGCAGTTGAATCTGCAATTTGGGACCCGCGATCTGGTCGGTTTTGGCGTCGAGAACGCGCCGCGCGGACTTTGtgc                       <  1:2306111/140‑1 (MQ=255)
                                                                                                                ttGAAGGCCGTCGCGGCCTGCGCCTTCGCCCGCTGTGGGAGTTTGAAATCGACACCGCGCGCCAGCAGTTGAATCTGCAATTTGGGACCCGCGATCTGGTCGGTTTTGGCGTCGAGAACGCGCCGCGCGGACTTTGtgct                      <  1:772038/140‑1 (MQ=255)
                                                                                                                 tGAAGGCCGTCGCGGCCTGCGCCTTCGCCCGCTGTGGGAGTTTGAAATCGACACCGCGCGCCAGCAGTTGAATCTGCAATTTGGGACCCGCGATCTGGTCGGTTTTGGCGTCGAGAACGCGCCGCGCGGACTTTGtgctg                     <  2:352825/140‑1 (MQ=255)
                                                                                                                  gAAGGCCGTCGCGGCCTGCGCCTTCGCCCGCTGTGGGAGTTTGAAATCGACACCGCGCGCCAGCAGTTGAATCTGCAATTTGGGACCCGCGATCTGGTCGGTTTTGGCGTCGAGAACGCGCCGCGCGGACTTTGtgctgc                    <  1:2097673/140‑1 (MQ=255)
                                                                                                                   aaGGCCGTCGCGGCCTGCGCCTTCGCCCGCTGTGGGAGTTTGTAATCGACACCGCGCGCCAGCAGTTGAATCTGCAATTTGGGACCCGCGATCTGGTCGGTTTTGGCGTCGAGAACGCGCCGCGCGGACTTTGTGCTGcc                   >  2:743521/1‑140 (MQ=255)
                                                                                                                   aaGGCCGTCGCGGCCTGCGCCTTCGCCCGCTGTGGGAGTTTGAAATCGACACCGCGCGCCAGCAGTTGAATCTGCAATTTGGGACCCGCGATCTGGTCGGTTTTGGCGTCGAGAACGCGCCGCGCGGACTTTGTGCTGcc                   >  1:1935245/1‑140 (MQ=255)
                                                                                                                     ggCCGTCGCGGCCTGCGCCTTCGCCCGCTGTGGGAGTTTGAAATCGACACCGCGCGCCAGCAGTTGAATCTGCAATTTGGGACCCGCGATCTGGTCGGTTTTGGCGTCGAGAACGCGCCGCGCGGACTTTGTGCTGCCgg                 <  2:935459/140‑1 (MQ=255)
                                                                                                                     ggCCGTCGCGGCCTGCGCCTTCGCCCGCTGTGGGAGTTTGAAATCGACACCGCGCGCCAGCAGTTGAATCTGCAATTTGGGACCCGCGATCTGGTCGGTTTTGGCGTCGAGAACGCGCCGCGCGGACTTTGTGCTGCCgg                 <  1:1345492/140‑1 (MQ=255)
                                                                                                                     ggCCGTCGCGGCCTGCGCCTTCGCCCGCTGTGGGAGTTTGAAATCGACACCGCGCGCCAGCAGTTGAATCTGCAATTTGGGACCCGCGATCTGGTCGGTTTTGGCGTCGAGAACGCGCCGCGCGGACTTTGTGCTGCCgg                 <  2:561903/140‑1 (MQ=255)
                                                                                                                     ggCCGTCGCGGCCTGCGCCTTCGCCCGCTGTGGGAGTTTGAAATCGACACCGCGCGCCAGCAGTTGAATCTGCAATTTGGGACCCGCGATCTGGTCGGTTTTGGCGTCGAGAACGCGCCGCGCGGACTTTGTGCTGCCgg                 >  2:39335/1‑140 (MQ=255)
                                                                                                                      gCCGTCGCGGCCTGCGCCTTCGCCCGCTGTGGGAGTTTGAAATCGACACCGCGCGCCAGCAGTTGAATCTGCAATTTGGGACCCGCGATCTGGTCGGTTTTGGCGTCGAGAACGCGCCGCGCGGACTTTGTGCTGCCGGt                >  1:842742/1‑140 (MQ=255)
                                                                                                                       ccGTCGCGGCCTGCGCCTTCGCCCGCTGTGGGAGTTTGAAATCGACACCGCGCGCCAGCAGTTGAATCTGCAATTTGGGACCCGCGATCTGGTCGGTTTTGGCGTCGAGAACGCGCCGCGCGGACTTTGTGCTGCCGGtt               <  1:1651485/140‑1 (MQ=255)
                                                                                                                         gTCGCGGCCTGCGCCTTCGCCCGCTGTGGGAGTTTGAAATCGACACCGCGCGCCAGCAGTTGAATCTGCAATTTGGGACCCGCGATCTGGTCGGTTTTGGCGTCGAGAACGCGCCGCGCGGACTTTGTGCTGCCGGTTGt             <  1:1379535/140‑1 (MQ=255)
                                                                                                                          tCGCGGCCTGCGCCTTCGCCCGCTGTGGGAGTTTGAAATCGACACCGCGCGCCAGCAGTTGAATCTGCAATTTGGGACCCGCGATCTGGTCGGTTTTGGCGTCGAGAACGCGCCGCGCGGACt                             >  1:972858/1‑123 (MQ=255)
                                                                                                                          tCGCGGCCTGCGCCTTCGCCCGCTGTGGGAGTTTGAAATCGACACCGCGCGCCAGCAGTTGAATCTGCAATTTGGGACCCGCGATCTGGTCGGTTTTGGCGTCGAGAACGCGCCGCGCGGACTTTGTGCTGCCGGTTGTc            >  2:1698931/1‑140 (MQ=255)
                                                                                                                          tCGCGGCCTGCGCCTTCGCCCGCTGCGGGAGTTTGAAATCGACACCGCGCGCCAGCAGTTGAATCTGCAATTTGGGACCCGCGATCTGGTCGGTTTTGGCGTCGAGAACGCGCCGCGCGGACTTTGTGCTGCCGGTTGTc            >  2:1261333/1‑140 (MQ=255)
                                                                                                                               gCCTGCGCCTTCGCCCGCTGTGGGAGTTTGAAATCGACACCGCGCGCCAGCAGTTGAATCTGCAATTTGGGACCCGCGATCTGGTCGGTTTTGGCGTCGAGAACGCGCCGCGCGGACTTTGTGCTGCCGGTTGTCTGTTg       <  1:1132778/140‑1 (MQ=255)
                                                                                                                               gCCTGCGCCTTCGCCCGCTGTGGGAGTTTGAAATCGACACCGCGCGCCAGCAGTTGAATCTGCAATTTGGGACCCGCGATCTGGTCGGTTTTGGCGTCGAGAACGCGCCGCGCGGACTTTGTGCTGCCGGTTGTCTGTTg       <  1:1131085/140‑1 (MQ=255)
                                                                                                                                 cTGCGCCTTCGCCCGCTGTGGGAGTTTGAAATCGACACCGCGCGCCAGCAGTTGAATCTGCAATTTGGGACCCGCGATCTGGTCGGTTTTGGCGTCGAGAACGCGCCGCGCGGACTTTGTGCTGCCGGTTGTCTGTTGCa     <  2:1090872/140‑1 (MQ=255)
                                                                                                                                 cTGCGCCTTCGCCCGCTGTGGGAGTTTGAAATCGACACCGCGCGCCAGCAGTTGAATCTGCAATTTGGGACCCGCGATCTGGTCGGTTTTGGCGTCGAGAACGCGCCGCGCGGACTTTGTGCTGCCGGTTGTCTGTTGCa     <  1:39335/140‑1 (MQ=255)
                                                                                                                                   gcgcCTTCGCCCGCTGTGGGAGTTTGAAATCGACACCGCGCGCCAGCAGTTGAATCTGCAATTTGGGACCCGCGATCTGGTCGGTTTTGGCGTCGAGAACGCGCCGCGCGGACTTTGTGCTGCCGGTTGTCTGTTGCAGt   <  1:1117824/140‑1 (MQ=255)
                                                                                                                                    cgcCTTCGCCCGCTGTGGGAGTTTGAAATCGACACCGCGCGCCAGCAGTTGAATCTGCAATTTGGGACCCGCGATCTGGTCGGTTTTGGCGTCGAGAACGCGCCGCGCGGACTTTGTGCTGCCGGTTGTCTGTTGCAGTa  >  1:1464073/1‑140 (MQ=255)
                                                                                                                                    cgcCTTCGCCCGCTGTGGGAGTTTGAAATCGACACCGCGCGCCAGCAGTTGAATCTGCAATTTGGGACCCGCGATCTGGTCGGTTTTGGCGTCGAGAACGCGCCGCGCGGACTTTGTGCTGCCGGTTGTCTGTTGCAGTa  >  2:621947/1‑140 (MQ=255)
                                                                                                                                    cgcCTTCGCCCGCTGTGGGAGTTTGAAATCGACACCGCGCGCCAGCAGTTGAATCTGCAATTTGGGACCCGCGATCTGGTCGGTTTTGGCGTCGAGAACGCGCCGCGCGGACTTTGTGCTGCCGGTTGTCTGTTGCAGTa  >  2:2129142/1‑140 (MQ=255)
                                                                                                                                    cgcCTTCGCCCGCTGTGGGAGTTTGAAATCGACACCGCGCGCCAGCAGTTGAATCTGCAATTTGGGACCCGCGATCTGGTCGGTTTTGGCGTCGAGAACGCGCCGCGCGGACTTTGTGCTGCCGGTTGTCTGTTGCAGTa  >  2:1147259/1‑140 (MQ=255)
                                                                                                                                    cgcCTTCGCCCGCTGTGGGAGTTTGAAATCGACACCGCGCGCCAGCAGTTGAATCTGCAATTTGGGACCCGCGATCTGGTCGGTTTTGGCGTCGAGAACGCGCCGCGCGGACTTTGTGCTGCCGGTTGTCTGTTGCAGTa  >  1:2057896/1‑140 (MQ=255)
                                                                                                                                        |                                                                                                                                       
GGGCGTTTTCGCCTGAGCGAACCGGCTGACCGCGAAACGATGGCGGCAGAACTGCAACGCACTAATCCTGCGGAACTGCTGTATGCAGAAGATTTTGCTGAAATGTCGTTAATTGAAGGCCGTCGCGGCCTGCGCCGTCGCCCGCTGTGGGAGTTTGAAATCGACACCGCGCGCCAGCAGTTGAATCTGCAATTTGGGACCCGCGATCTGGTCGGTTTTGGCGTCGAGAACGCGCCGCGCGGACTTTGTGCTGCCGGTTGTCTGTTGCAGTA  >  NC_000913/2857549‑2857820

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑