Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,604,3440CT30.6% 251.7 / 82.4 160S62F (TCT→TTT) bglJDNA‑binding transcriptional regulator BglJ
Reads supporting (aligned to +/- strand):  ref base C (32/79);  new base T (49/0);  total (81/79)
Fisher's exact test for biased strand distribution p-value = 9.50e-20
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 182 total aligned reads displayed.

ATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAG‑CGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAG‑TTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCAT  >  NC_000913/4604207‑4604481
                                                                                                                                         |                                                                                                                                           
aTGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATtttttctt                                                                                                                                           >  1:505180/1‑140 (MQ=255)
     tCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTttttctttttct                                                                                                                                      <  1:570413/140‑1 (MQ=255)
     tCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTttttctttttct                                                                                                                                      <  1:1534121/140‑1 (MQ=255)
     tCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTttttctttttct                                                                                                                                      <  2:2246838/140‑1 (MQ=255)
                 tCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGt                                                                                                                          <  1:812994/140‑1 (MQ=255)
                   ggTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTTTTCCATGAGAAGTgg                                                                                                                        >  1:2097576/1‑139 (MQ=255)
                    gTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTTCCCTGAGAAGTGAg                                                                                                                       >  2:428559/1‑140 (MQ=255)
                    gTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAg                                                                                                                       >  1:1080658/1‑140 (MQ=255)
                      aTTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTGCCATGAGAAGTGGg‑cg                                                                                                                    >  2:1288062/1‑140 (MQ=255)
                      aTTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAg‑cg                                                                                                                    >  1:1367536/1‑140 (MQ=255)
                        tGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTTTACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAG‑CGCa                                                                                                                  <  1:295436/140‑1 (MQ=255)
                        tGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAG‑CGCa                                                                                                                  <  1:2654339/140‑1 (MQ=255)
                        tGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAG‑CGCa                                                                                                                  <  1:369647/140‑1 (MQ=255)
                        tGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAG‑CGCa                                                                                                                  <  1:965998/140‑1 (MQ=255)
                        tGAGAGTTTATCCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCCTTTTTTTTTTTTTTTCCCTTAGAAGTGGg‑cgcc                                                                                                                  >  2:288416/1‑139 (MQ=255)
                         gagaGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCTGCTTTTATTTTTCTGCCATGAGAAGTGAG‑CGCag                                                                                                                 >  1:1018415/1‑140 (MQ=255)
                         gagaGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTTTTGCCTGAGAAGTGAG‑CGCag                                                                                                                 >  2:630110/1‑140 (MQ=255)
                         gagaGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAG‑CGCag                                                                                                                 >  1:1868099/1‑140 (MQ=255)
                         gagaGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAG‑CGCag                                                                                                                 <  2:1787157/140‑1 (MQ=255)
                         gagaGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAG‑CGCag                                                                                                                 <  1:1435666/140‑1 (MQ=255)
                         gagaGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAG‑CGCag                                                                                                                 >  2:1133953/1‑140 (MQ=255)
                         gagaGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAG‑CGCag                                                                                                                 >  1:289356/1‑140 (MQ=255)
                          agagTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAG‑CGCaga                                                                                                                <  1:2390040/140‑1 (MQ=255)
                           gagTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAG‑CGCagag                                                                                                               <  1:790012/140‑1 (MQ=255)
                            agTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAG‑CGCagaga                                                                                                              <  2:2525480/140‑1 (MQ=255)
                               ttATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTTCCATGAGGAGTGAG‑AGCCGAGAggg                                                                                                           >  2:2394969/1‑140 (MQ=255)
                               ttATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAG‑CGCAGAGAggg                                                                                                           <  2:2809425/140‑1 (MQ=255)
                               ttATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTATGCGGCGGTCTGTTTTTATTTTTTTTCCATGAGAAGTGAG‑CGCAGAGAggg                                                                                                           >  1:656043/1‑140 (MQ=255)
                                   tCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAG‑CGCAGAGAGGGATTa                                                                                                       <  2:1534626/140‑1 (MQ=255)
                                      gAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTGCCATGAGAAAGGAG‑GGCAGAGAGGGGTTATCt                                                                                                    >  2:2056951/1‑140 (MQ=255)
                                        aaGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAG‑CGCAGAGAGGGATTATCTTg                                                                                                  <  1:2394969/140‑1 (MQ=255)
                                            ttGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAG‑CGCAGAGAGGGATTATCTTGCctg                                                                                              >  1:2007338/1‑140 (MQ=255)
                                             tGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAG‑CGCAGAGAGGGATTATCTTGCctga                                                                                             >  1:221806/1‑140 (MQ=255)
                                                 gacagcccTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAG‑CGCAGAGAGGGATTATCTTGCctgactga                                                                                         <  1:1031079/135‑1 (MQ=255)
                                                   tAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTGATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAG‑CGCAGAGAGGGATTATCTTGCCTGACTGAAc                                                                                       <  1:1749213/140‑1 (MQ=255)
                                                   tAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAG‑CGCAGAGAGGGATTATCTTGCCTGACTGAAc                                                                                       <  2:1367536/140‑1 (MQ=255)
                                                   tAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAG‑CGCAGAGAGGGATTATCTTGCCTGACTGAAc                                                                                       <  2:1566877/140‑1 (MQ=255)
                                                   tAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTCTTCTGCCATGAGAAGTGAG‑CGCAGAGAGGGATTATCTTGCCTGACTGAAc                                                                                       <  1:101217/140‑1 (MQ=255)
                                                     aCCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTGCCATGAGAAGGGAG‑CGCAGAGAGGGAGTATCTTGCCTGACTGAACTg                                                                                     >  2:959027/1‑140 (MQ=255)
                                                     aCCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTCTTCCATGAGAAGGGAG‑CGCAGAGAGGGGTTATTTTTCCTGACTGAACTg                                                                                     >  2:101217/1‑140 (MQ=255)
                                                       ccTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAG‑CGCAGAGAGGGATTATCTTGCCTGACTGAACTGGc                                                                                   <  1:1133953/140‑1 (MQ=255)
                                                           ataAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAG‑CGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGAtt                                                                               <  2:1868099/140‑1 (MQ=255)
                                                           ataAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAG‑CGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGAtt                                                                               <  1:2047395/140‑1 (MQ=255)
                                                            taAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTGCCCTGAGAAGTGAG‑CGCAGAGAGGGGTTATCTTGCCTGACTGGACTGGCGGTTa                                                                              >  2:2615774/1‑140 (MQ=255)
                                                               gCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAG‑CGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAG‑t                                                                          <  2:505180/140‑1 (MQ=255)
                                                                 tCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTGCCATGAGAAGTGAG‑CGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAG‑ttt                                                                        >  2:1150874/1‑140 (MQ=255)
                                                                 tCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTCTGCCATGAGAAGGGAG‑CGCAGAGAGGGATTTTTTTTCCTGACTGAAATGGCGAGTAAG‑ttt                                                                        >  2:1049999/1‑140 (MQ=255)
                                                                     tACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTTTTCCCTGAGAAGTGAG‑CGGAGGGGGGGATTATCTTGCCTGACTGAACTGGCGGTTTAG‑ATTCcgc                                                                    >  2:2909716/1‑140 (MQ=255)
                                                                           taCCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCCTTTTTTTTTTTTCTTCTATGGGAAGTGAG‑AGCACGGAGGGCGTATCTTGCCTGACTGAACTTGCGAGTAAGCTTTCCG‑GTAccc                                                              >  2:358969/1‑140 (MQ=255)
                                                                           taCCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTTCCATGAGAAGTGAG‑CGCAGAGAGGGGTTATCTTGCCTGACTGAAATGGCGGTTAAG‑GTTTCGCGTAacc                                                              >  2:837018/1‑140 (MQ=255)
                                                                           taCCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTTCCATGAGAAGTGAG‑CGCCGAGGGGGGTTATCTTGCCTGACTGAACTGGGGGTTAAG‑TTTTCGCGGAccc                                                              >  2:386148/1‑140 (MQ=255)
                                                                           taCCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGCCATTTTTTCTTTTTCTGCCATGGGAAGTGAG‑GGGAGAGAGGGGTTATCTTGGCTGACTGAAATGGCGGTTAAA‑GTTCCGCGTAccc                                                              >  2:2373436/1‑140 (MQ=255)
                                                                            aCCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAG‑CGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAG‑TTTCCGCGTACCCg                                                             <  1:2542857/140‑1 (MQ=255)
                                                                             ccAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAG‑CGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAG‑TTTCCGCGTGCCCgg                                                            <  1:1970428/140‑1 (MQ=255)
                                                                             ccAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAG‑CGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAG‑TTTCCGCGTACCCgg                                                            >  1:2042562/1‑140 (MQ=255)
                                                                             ccAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAG‑CGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAG‑TTTCCGCGTACCCgg                                                            <  1:2195855/140‑1 (MQ=255)
                                                                               aGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTTCCCTGAGAAGTGAG‑CGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAG‑TTTTCGCGTACCCcgcg                                                          >  2:1400104/1‑140 (MQ=255)
                                                                               aGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAG‑CGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAG‑TTTCCGCGTACCCGGCg                                                          <  1:1319859/140‑1 (MQ=255)
                                                                               aGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAG‑CGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAG‑TTTCCGCGTACCCGGCg                                                          <  1:2615774/140‑1 (MQ=255)
                                                                                gTCAGGAGTCATTTCAGGATGTCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTGCCCTGAGAAGTGAG‑GGCAGAGAGGGGTTATCTTGCCTGACTTAACTGGCGGTTAAA‑TTTTCGCGTACCCcgcgg                                                         >  2:665536/1‑139 (MQ=255)
                                                                                gTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTTCCCTGAGAAGTGAG‑CGCAGAGAGGGATTATCTTTCCTGACTGAACTGGCGATTTAA‑TTTTCGCGGACCCcgcgg                                                         >  2:2195855/1‑139 (MQ=255)
                                                                                gTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTTCCCTGAGAAGGGAG‑CGCCGAGAGGGATTATCTTTCCTGACTGAACTGGCGATTAAG‑TTTTCGCGTACCCcgcgg                                                         >  2:1811436/1‑139 (MQ=255)
                                                                                gTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTCTTCCATGAGAAGTGAG‑CGCAGAGAGGGGTTATTTTTCCTGACTGAACTGGCGATTAAA‑TTTCCGCGTACCCGGCgg                                                         >  2:850529/1‑139 (MQ=255)
                                                                                 tCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTGATTTTTTTTTTTTCTGGCTTAGGAAGTGGG‑GGCAGGGAGGGCGTATATTGCCTGATTGAACCCGGGAATAAG‑TTTCCGCGTACCCGGCggt                                                        >  2:1208066/1‑138 (MQ=255)
                                                                                 tCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGGCATTTTTTTTTTTTCTGCCATGAGCAGGGAGCCGCA‑AGAGGGGTTCTATTACCTCGATAAACAGGCGCTTAAG‑TTTTCCCGTCCCCCGGGtt                                                        >  2:2693685/1‑140 (MQ=255)
                                                                                  cAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTTCCATGAGAAGTGAG‑CGCAGAGAGGGGTTATTTTTCCTGACTGAACTGGCGGTTAAG‑TTTTCGCGGACCCGGCGgtt                                                       >  2:2251315/1‑140 (MQ=255)
                                                                                  cAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTCTGCCCTGAGAAATGAG‑CGCAGAGGGGGGTTATCTTTCCTGACTGAAATGGCGATTAAG‑TTTCCCCGTACCCGGCGttt                                                       >  2:2336606/1‑140 (MQ=255)
                                                                                  cAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAG‑CGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAG‑TTTCCGCGTAcc                                                               >  1:799832/1‑132 (MQ=255)
                                                                                  cAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAG‑CGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAG‑TTTCCGCGTAcc                                                               <  2:799832/132‑1 (MQ=255)
                                                                                  cAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAG‑CGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAG‑TTTCCGCGTACCCGGCGttt                                                       >  1:1628430/1‑140 (MQ=255)
                                                                                  cAGGAGTCATTTCAGGATACCATGTCGCGGATCTCGTTTGCGGCGGTCCTTTTTTTTTTTTTTTCCATGAGAAGGGAG‑CGCAGAGAGGGATTATCTTGCCTGACTGAACTGGGGGTTAAG‑TTTCCCCGTACCCGGGGttt                                                       >  2:1958965/1‑140 (MQ=255)
                                                                                   aGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTTCCATGAGAAGTGAG‑CGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAG‑TTTCCGCGTACCCGGCGTTTa                                                      >  2:1069321/1‑140 (MQ=255)
                                                                                   aGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAG‑CGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAG‑TTTCCGCGTAcc                                                               >  2:2831924/1‑131 (MQ=255)
                                                                                   aGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAG‑CGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAG‑TTTCCGCGTAcc                                                               <  1:2831924/131‑1 (MQ=255)
                                                                                    ggAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAG‑CGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAG‑TTTCCGCGTACCCGGCGTTTAg                                                     <  1:1811436/140‑1 (MQ=255)
                                                                                     gAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTTTTCCCTGAGAAGTGAG‑CGCAGAGAGGGGTTATCTTGCCCGACTGAACCGGCGATTAAG‑TTTCCCCGCCCCCCGCGCTTTGt                                                    >  2:786031/1‑140 (MQ=255)
                                                                                        tCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAG‑CGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAG‑TTTCCGCGTACCCGGCGTTTAGTTAt                                                 <  1:498390/140‑1 (MQ=255)
                                                                                        tCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAG‑CGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAG‑TTTCCGCGTACCCGGCGTTTAGTTAt                                                 <  1:2824224/140‑1 (MQ=255)
                                                                                          aTTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAG‑CGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAG‑TTTCCGCGTACCCGGCGTTTAGTTATTg                                               <  2:1436592/140‑1 (MQ=255)
                                                                                          aTTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAG‑CGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAG‑TTTCCGCGTACCCGGCGTTTAGTTATTg                                               <  1:669809/140‑1 (MQ=255)
                                                                                            ttCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAG‑CGCAGAGAGGGGTTATCTTGCCTGACTGAACTGGCGATTAAG‑TTTCCGCGTACCCGGCGTTTAGTTATTGCg                                             <  1:2247992/140‑1 (MQ=255)
                                                                                            ttCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAG‑CGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAG‑TTTCCGCGTACCCGGCGTTTAGTTATTGCg                                             <  2:1628430/140‑1 (MQ=255)
                                                                                            ttCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAG‑CGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAG‑TTTCCGCGTACCCGGCGTTTAGTTATTGCg                                             <  1:1178301/140‑1 (MQ=255)
                                                                                            ttCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAG‑CGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAG‑TTTCCGCGTACCCGGCGTTTAGTTATTGCg                                             <  2:744830/140‑1 (MQ=255)
                                                                                             tCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTGCCATGAGAAGTGAG‑CGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAG‑TTTCCGCGTACCCGGCGTTTAGTTATTGCgg                                            >  2:460375/1‑140 (MQ=255)
                                                                                               aGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAG‑CGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAG‑TTTCCGCGTACCCGGCGTTTAGTTATTGCGgat                                          <  2:347963/140‑1 (MQ=255)
                                                                                                 gATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAG‑CGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAG‑TTTCCGCGTACCCGGCGTTTAGTTATTGCGgatga                                        <  1:412387/140‑1 (MQ=255)
                                                                                                    gCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAG‑CGCAGAGAGGGGGTATCTTGCCTGGCTGAACTGGGGATTAAA‑GTTTCGCGTACCCGGCGTTTAGTTATTTCGgatggtga                                     >  2:2725232/1‑140 (MQ=255)
                                                                                                      cATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTTCCATGAGAAGGGAG‑CGCAGAGAGGGATTATTTTTCCTGACTGAACTGGCGATTAAG‑TTTTCCCGGACCCCGCGTTTTGTTATTGCGgatgatgaga                                   >  2:2941389/1‑138 (MQ=255)
                                                                                                         gTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAG‑CGCAGAGAGGGATTATCTTGCCTGACTTAACTGGCGATTAAG‑TTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTg                                <  2:2073598/140‑1 (MQ=255)
                                                                                                               gATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTGCCATGAGAAGTGAG‑CGCAGAGAGGGGTTATCTTGCCTGACTGAACTGGCGATTAAG‑TTTCCGCGTACCCGGCGGTTTGTTATTGCGGGTGATGATATTGAAGCTc                          >  2:794109/1‑140 (MQ=255)
                                                                                                                 tctcGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAG‑CGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAG‑TTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCgg                        <  2:2042562/140‑1 (MQ=255)
                                                                                                                     gTTTGCGGCGGTCATTTTTTTTTTTTCTGCCATGAGAAGTGAG‑CGCAGAGAGGGGTTATCTTGCCTGACTGAACTGGCGATTAAG‑TTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGGTATTGTAGCTCGGCTgg                    >  2:1343961/1‑139 (MQ=255)
                                                                                                                     gTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAG‑CGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAG‑TTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGa                    <  1:2941389/140‑1 (MQ=255)
                                                                                                                               gTCGTTTTTTTTTTTTCTTCCCTGGGGAGTGAG‑CGCACAGAGGGAGTATCTTTCCTGACTGAAATGGCGATTAAG‑TTTCCGCGGACCCGGCGTTTTGTTATTGCGGATGATGATATTGAAACTCGGCCGGTTGGTTCAtt          >  2:1339345/1‑140 (MQ=255)
                                                                                                                               gTCATTTTTTCTTTTTCTGCCATGAGAAGTGAG‑CGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAG‑TTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCAtt          >  2:1087753/1‑140 (MQ=255)
                                                                                                                               gTCATTTTTTCTTTTTCTGCCATGAGAAGGGAG‑CGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAG‑TTTCCGCGTACCCGGCGGTTTGTTATTGCGGGTGATGATATTTAAGCTCGGCTGGTTGGTTCAtt          >  2:2410659/1‑140 (MQ=255)
                                                                                                                                     ttttctttttctGCCATGAGAAGTGAG‑CGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAG‑TTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGcc    <  1:860431/140‑1 (MQ=255)
                                                                                                                                       ttctttttctGCCATGAGAAGTGAG‑CGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAG‑TTTCCGCGTGCCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCAt  <  1:395547/140‑1 (MQ=255)
                                                                                                                                       ttctttttctGCCATGAGAAGTGAG‑CGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAG‑TTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCAt  <  2:2007338/140‑1 (MQ=255)
                                                                                                                                         |                                                                                                                                           
ATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAG‑CGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGATTAAG‑TTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCAT  >  NC_000913/4604207‑4604481

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑