Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,260,694 C→A P239P (CCG→CCT)  fruA ← fructose‑specific PTS multiphosphoryl transfer protein FruA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,260,6940CA99.1% 403.4 / ‑3.0 114P239P (CCG→CCT) fruAfructose‑specific PTS multiphosphoryl transfer protein FruA
Reads supporting (aligned to +/- strand):  ref base C (0/1);  new base A (59/54);  total (59/55)
Fisher's exact test for biased strand distribution p-value = 4.82e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.61e-01

Only 100 of 119 total aligned reads displayed.

GTACCGGCACCATCAGCGCAAAGGCTGAACCACCACCAATCTGCATCAGCGCCGCAGCCAACGTACCCGGCTCTTTAAACGCTTCGATACCAAAAGCAAAAGAAAGCGCGATACACAGACCACCTGCAACGACCATCGGCAGCATGTAAGAAACGCCCGTCAGCAAGTGACGGTATGCGCCTGCACTCTCTTTCTTACTTTCAGTGGTCGCCGTTTGAGCTTTGCCCGCCGGTTCATACGGCGTTGCTTCAGCAACCGCTTTATCCAGTTCCT  >  NC_000913/2260558‑2260830
                                                                                                                                        |                                                                                                                                        
gTACCGGCACCATCAGCGCAAAGGCTGAACCACCACCAATCTTCATCAGCGCCGCAGCCAACGTACCCGGCTCTTTAAACGCTTCGATACCAAAAGCAAAAGAAAGCGCGATACACAGACCACCTGCAACGACCATAGgc                                                                                                                                       <  2:2653449/140‑1 (MQ=255)
gTACCGGCACCATCAGCGCAAAGGCTGAACCACCACCAATCTGCATCAGCGCCGCAGCCAACGTACCCGGCTCTTTAAACGCTTCGATACCAAAAGCAAAAGAAAGCGCGATACACAGACCACCTGCAACGACCATCGgc                                                                                                                                       <  1:948728/140‑1 (MQ=255)
 tACCGGCACCATCAGCGCAAAGGCTGAACCACCACCAATCTGCATCAGCGCCGCAGCCAACGTACCCGGCTCTTTAAACGCTTCGATACCAAAAGCAAAAGAAAGCGCGATACACAGACCACCTGCAACGACCATAGgca                                                                                                                                      <  1:2855589/140‑1 (MQ=255)
 tACCGGCACCATCAGCGCAAAGGCTGAACCACCACCAATCTGCATCAGCGCCGCAGCCAACGTACCCGGCTCTTTAAACGCTTCGATACCAAAAGCAAAAGAAAGCGCGATACACAGACCACCTGCAACGACCATAGgca                                                                                                                                      <  1:2957327/140‑1 (MQ=255)
  aCCGGCACCATCAGCGCAAAGGCTGAACCACCACCAATCTGCATCAGCGCCGCAGCCAACGTACCCGGCTCTTTAAACGCTTCGATACCAAAAGCAAAAGAAAGCGCGATACACAGACCACCTGCAACGACCATAGgcag                                                                                                                                     <  1:275815/140‑1 (MQ=255)
  aCCGGCACCATCAGCGCAAAGGCTGAACCACCACCAATCTGCATCAGCGCCGCAGCCAACGTACCCGGCTCTTTAAACGCTTCGATACCAAAAGCAAAAGAAAGCGCGATACACAGACCACCTGCAACGACCATAGgcag                                                                                                                                     <  2:1230682/140‑1 (MQ=255)
  aCCGGCACCATCAGCGCAAAGGCTGAACCACCACCAATCTGCATCAGCGCCGCAGCCAACGTACCCGGCTCTTTAAACGCTTCGATACCAAAAGCAAAAGAAAGCGCGATACACAGACCACCTGCAACGACCATAGgcag                                                                                                                                     >  1:1620576/1‑140 (MQ=255)
  aCCGGCACCATCAGCGCAAAGGCTGAACCACCACCAAGCTGCATCAGCGCCGCAGCCAACGTACCCGGCTCTTTAAACGCTTCGATACCAAAAGCAAAAGAAAGCGCGATACACAGACCACCTGCAACGACCATAGgcag                                                                                                                                     <  2:2552488/140‑1 (MQ=255)
   ccGGCACCATCAGCGCAAAGGCTGAACCACCACCAATCTGCATCAGCGCCGCAGCCAACGTACCCGGCTCTTTAAACGCTTCGATACCAAAAGCAAAAGAAAGCGCGATACACAGACCACCTGCAACGACCATAGgcagc                                                                                                                                    <  2:1105971/140‑1 (MQ=255)
      gCACCATCAGCGCAAAGGCTGAACCACCACCAATCTGCATCAGCGCCGCAGCCAACGTACCCGGCTCTTTAAACGCTTCGATACCAAAAGCAAAAGAAAGCGCGATACACAGACCACCTGCAACGACCATAGGCAGCATg                                                                                                                                 >  1:1400969/1‑140 (MQ=255)
       cACCATCAGCGCAAAGGCTGAACCACCACCAATCTGCATCAGCGCCGCAGCCAACGTACCCGGCTCTTTAAACGCTTCGATACCAAAAGCAAAAGAAAGCGCGATACACAGACCACCTGCAACGACCATAGGCAGCACGt                                                                                                                                >  1:1000139/1‑140 (MQ=255)
          cATCAGCGCAAAGGCTGAACCACCACCAATCTGCATCAGCGCCGCAGCCAACGTACCCGGCTCTTTAAACGCTTCGATACCAAAAGCAAAAGAAAGCGCGATACACAGACCACCTGCAACGACCATAGGCAGCATGTAAg                                                                                                                             >  1:12449/1‑140 (MQ=255)
            tCAGCGCAAAGGCTGAACCACCACCAATCTGCATCAGCGCCGCAGCCAACGTACCCGGCTCTTTAAACGCTTCGATACCAAAAGCAAAAGAAAGCGCGATACACAGACCACCTGCAACGACCATAGGCAGCATGTAAGaa                                                                                                                           >  2:2728855/1‑140 (MQ=255)
            tCAGCGCAAAGGCTGAACCACCACCAATCTGCATCAGCGCCGCAGCCAACGTACCCGGCTCTTTAAACGCTTCGATACCAAAAGCAAAAGAAAGCGCGATACACAGACCACCTGCAACGACCATAGGCAGCATGTAAGaa                                                                                                                           >  2:2747512/1‑140 (MQ=255)
             cAGCGCAAAGGCTGAACCACCACCAATCTGCATCAGCGCCGCAGCCAACGTACCCGGCTCTTTAAACGCTTCGATACCAAAAGCAAAAGAAAGCGCGATACACAGACCACCTGCAACGACCATAGGCAGCATGTAAGaaa                                                                                                                          <  1:2747512/140‑1 (MQ=255)
             cAGCGCAAAGGCTGAACCACCACCAATCTGCATCAGCGCCGCAGCCAACGTACCCGGCTCTTTAAACGCTTCGATACCAAAAGCAAAAGAAAGCGCGATACACAGACCACCTGCAACGACCATAGGCAGCATGTAAGaaa                                                                                                                          <  2:12449/140‑1 (MQ=255)
              aGCGCAAAGGCTGAACCACCACCAATCTGCATCAGCGCCGCAGCCAACGTACCCGGCTCTTTAAACGCTTCGATACCAAAAGCAAAAGAAAGCGCGATACACAGACCACCTGCAACGACCATAGGCAGCATGTAAGAAAc                                                                                                                         >  2:1525239/1‑140 (MQ=255)
                cgcAAAGGCTGAACCACCACCAATCTGCATCAGCGCCGCAGCCAACGTACCCGGCTCTTTAAACGCTTCGATACCAAAAGCAAAAGAAAGCGCGATACACAGACCACCTGCAACGACCATAGgcag                                                                                                                                     <  2:685022/126‑1 (MQ=255)
                cgcAAAGGCTGAACCACCACCAATCTGCATCAGCGCCGCAGCCAACGTACCCGGCTCTTTAAACGCTTCGATACCAAAAGCAAAAGAAAGCGCGATACACAGACCACCTGCAACGACCATAGgcag                                                                                                                                     >  1:685022/1‑126 (MQ=255)
                cgcAAAGGCTGAACCACCACCAATCTGCATCAGCGCCGCAGCCAACGTACCCGGCTCTTTAAACGCTTCGATACCAAAAGCAAAAGAAAGCGCGATACACAGACCACCTGCAACGACCATAGGCAGCATGTAAGAAACGc                                                                                                                       <  1:1276930/140‑1 (MQ=255)
                cgcAAAGGCTGAACCACCACCAATCTGCATCAGCGCCGCAGCCAACGTACCCGGCTCTTTAAACGCTTCGATACCAAAAGCAAAAGAAAGCGCGATACACAGACCACCTGCAACGACCATAGGCAGCATGTAAGAAACGc                                                                                                                       <  2:800904/140‑1 (MQ=255)
                cgcAAAGGCTGAACCACCACCAATCTGCATCAGCGCCGCAGCCAACGTACCCGGCTCTTTAAACGCTTCGATACCAAAAGCAAAAGAAAGCGCGATACACAGACCACCTGCAACGACCATAGGCAGCATGTAAGAAACGc                                                                                                                       >  2:1349854/1‑140 (MQ=255)
                cgcAAAGGCTGAACCACCACCAATCTGCATCAGCGCCGCAGCCAACGTACCCGGCTCTTTAAACGCTTCGATACCAAAAGCAAAAGAAAGCGCGATACACAGACCACCTGCAACGACCATAGGCAGCATGTAAGAAACGc                                                                                                                       >  2:1276930/1‑140 (MQ=255)
                 gcAAAGGCTGAACCACCACCAATCTGCATCAGCGCCGCAGCCAACGTACCCGGCTCTTTAAACGCTTCGATACCAAAAGCAAAAGAAAGCGCGATACACAGACCACCTGCAACGACCATAGGCAGCATGTAAGAAACGcc                                                                                                                      >  2:2725033/1‑140 (MQ=255)
                   aaaGGCTGAACCACCACCAATCTGCATCAGCGCCGCAGCCAACGTACCCGGCTCTTTAAACGCTTCGATACCAAAAGCAAAAGAAAGCGCGATACACAGACCACCTGCAACGACCATAGGCAGCATGTAAGAAACGCCCg                                                                                                                    <  2:429777/140‑1 (MQ=255)
                    aaGGCTGAACCACCACCAATCTGCATCAGCGCCGCAGCCAACGTACCCGGCTCTTTAAACGCTTCGATACCAAAAGCAAAAGAAAGCGCGATACACAGACCACCTGCAACGTCCATAGGCAGCATGTAAGAAACGCCCGt                                                                                                                   <  1:1525239/140‑1 (MQ=255)
                    aaGGCTGAACCACCACCAATCTGCATCAGCGCCGCAGCCAACGTACCCGGCTCTTTAAACGCTTCGATACCAAAAGCAAAAGAAAGCGCGATACACAGACCACCTGCAACGACCATAGGCAGCATGTAAGAAACGCCCGt                                                                                                                   <  1:2743851/140‑1 (MQ=255)
                      ggCTGAACCACCACCAATCTGCATCAGCGCCGCAGTCAACGTACCCGGCTCTTTAAACGCTTCGATACCAAAAGCAAAAGAAAGCGCGATACACAGACCACCTGCAACGACCATAGGCATCATGTAAGAAACGCCCGTCa                                                                                                                 <  2:447158/140‑1 (MQ=255)
                      ggCTGAACCACCACCAATCTGCATCAGCGCCGCAGCCAACGTACCCGGCTCTTTAAACGCTTCGATACCAAAAGCAAAAGAAAGCGCGATACACAGACCACCTGCAACGACCATAGGCAGCATGTAAGAAACGCCCGTCa                                                                                                                 <  1:1932519/140‑1 (MQ=255)
                       gCTGAACCACCACCAATCTGCATCAGCGCCGCAGCCAACGTACCCGGCTCTTTAAACGCTTCGATACCAAAAGCAAAAGAAAGCGCGATACACAGACCACCTGCAACGACCATAGGCAGCATGTAAGAAACGCCCGTCAg                                                                                                                <  1:498747/140‑1 (MQ=255)
                        cTGAACCACCACCAATCTGCATCAGCGCCGCAGCCAACGTACCCGGCTCTTTAAACGCTTCGATACCAAAAGCAAAAGAAAGCGCGATACACAGACCACCTGCAACGACCATAGGCAGCATGTAAGAAACGCCCGTCAGc                                                                                                               <  2:303520/140‑1 (MQ=255)
                          gAACCACCACCAATCTGCATCAGCGCCGCAGCCAACGTACCCGGCTCTTTAAACGCTTCGATACCAAAAGCAAAAGAAAGCGCGATACACAGACCACCTGCAACGACCATAGGCAGCATGTAAGAAACGCCCGTCAGCaa                                                                                                             <  2:1620576/140‑1 (MQ=255)
                          gAACCACCACCAATCTGCATCAGCGCCGCAGCCAACGTACCCGGCTCTTTAAACGCTTCGATACCAAAAGCAAAAGAAAGCGCGATACACAGACCACCTGCAACGACCATAGGCAGCATGTAAGAAACGCCCGTCAGCaa                                                                                                             >  1:1028619/1‑140 (MQ=255)
                              caccaccaATCTGCATCAGCGCCGCAGCCAACGTACCCGGCTCTTTAAACGCTTCGATACCAAAAGCAAAAGAAAGCGCGATACACAGACCACCTGCAACGACCATAGGCAGCATGTAAGAAACGCCCGTCAGCAAGTGa                                                                                                         <  2:1400969/140‑1 (MQ=255)
                              caccaccaATCTGCATCAGCGCCGCAGCCAACGTACCCGGCTCTTTAAACGCTTCGATACCAAAAGCAAAAGAAAGCGCGATACACAGACCACCTGCAACGACCATAGGCAGCATGTAAGAAACGCCCGTCAGCAAGTGa                                                                                                         <  2:280918/140‑1 (MQ=255)
                              caccaccaATCTGCATCAGCGCCGCAGCCAACGTACCCGGCTCTTTAAACGCTTCGATACCAAAAGCAAAAGAAAGCGCGATACACAGACCACCTGCAACGACCATAGGCAGCATGTAAGAAACGCCCGTCAGCAAGTGa                                                                                                         <  1:2762714/140‑1 (MQ=255)
                              caccaccaATCTGCATCAGCGCCGCAGCCAACGTACCCGGCTCTTTAAACGCTTCGATACCAAAAGCAAAAGAAAGCGCGATACACAGACCACCTGCAACGACCATAGGCAGCATGTAAGAAACGCCCGTCAGCAAGTGa                                                                                                         <  1:2543606/140‑1 (MQ=255)
                              caccaccaATCTGCATCAGCGCCGCAGCCAACGTACCCGGCTCTTTAAACGCTTCGATACCAAAAGCAAAAGAAAGCGCGATACACAGACCACCTGCAACGACCATAGGCAGCATGTAAGAAACGCCCGTCAGCAAGTGa                                                                                                         <  2:494924/140‑1 (MQ=255)
                               accaccaATCTGCATCAGCGCCGCAGCCAACGTACCCGGCTCTTTAAACGCTTCGATACCAAAAGCAAAAGAAAGCGCGATACACAGACCACCTGCAACGACCATAGGCAGCATGTAAGAAACGCCCGTCAGCAAGTGAc                                                                                                        >  1:1853107/1‑140 (MQ=255)
                                ccaccaATCTGCATCAGCGCCGAAGCCAACGTACCCGGCTCTTTAAACGCTTCGATACCAAAAGCAAAAGAAAGCGCGATACACAGACCACCTGCAACGACCATAGGCAGCATGTAAGAAACGCCCGTCAGCAAGTGACg                                                                                                       <  2:1233366/140‑1 (MQ=255)
                                 caccaATCTGCATCAGCGCCGCAGCCAACGTACCCGGCTCTTTAAACGCTTCGATACCAAAAGCAAAAGAAAGCGCGATACACAGACCACCTGCAACGACCATAGGCAGCATGTAAGAAACGCCCGTCa                                                                                                                 <  2:1932465/129‑1 (MQ=255)
                                 caccaATCTGCATCAGCGCCGCAGCCAACGTACCCGGCTCTTTAAACGCTTCGATACCAAAAGCAAAAGAAAGCGCGATACACAGACCACCTGCAACGACCATAGGCAGCATGTAAGAAACGCCCGTCa                                                                                                                 >  1:1932465/1‑129 (MQ=255)
                                 caccaATCTGCATCAGCGCCGCAGCCAACGTACCCGGCTCTTTAAACGCTTCGATACCAAAAGCAAAAGAAAGCGCGATACACAGACCACCTGCAACGACCATAGGCAGCATGTAAGAAACGCCCGTCAGCAAGTGACgg                                                                                                      >  2:2409919/1‑140 (MQ=255)
                                 caccaATCTGCATCAGCGCCGCAGCCAACGTACCCGGCTCTTTAAACGCTTCGATACCAAAAGCAAAAGAAAGCGCGATACACAGACCACCTGCAACGACCATAGGCAGCATGTAAGAAACGCCCGTCAGCAAGTGACgg                                                                                                      >  1:310097/1‑140 (MQ=255)
                                 caccaATCTGCATCAGCGCCGCAGCCAACGTACCCGGCTCTTTAAACGCTTCGATACCAAAAGCAAAAGAAAGCGCGATACACAGACCACCTGCAACGACCATAGGCAGCATGTAAGAAACGCCCGTCAGCAAGTGACgg                                                                                                      >  2:1614811/1‑140 (MQ=255)
                                 caccaATCTGCATCAGCGCCGCAGCCAACGTACCCGGCTCTTTAAACGCTTCGATACCAAAAGCAAAAGAAAGCGCGATACACAGACCACCTGCAACGACCATAGGCAGCATGTAAGAAACGCCCGTCAGCAAGTGACgg                                                                                                      >  1:1078752/1‑140 (MQ=255)
                                 caccaATCTGCATCAGCGCCGCAGCCAACGTACCCGGCTCTTTAAACGCTTCGATACCAAAAGCAAAAGAAAGCGCGATACACAGACCACCTGCAACGACCATAGGCAGCATGTAAGAAACGCCCGTCAGCAAGTGACgg                                                                                                      >  2:2889161/1‑140 (MQ=255)
                                 caccaATCTGCATCAGCGCCGCAGCCAACGTACCCGGCTCTTTAAACGCTTCGATACCAAAAGCAAAAGAAAGCGCGATACACAGACCACCTGCAACGACCATAGGCAGCATGTAAGAAACGCCCGTCAGCAAGTGACgg                                                                                                      >  2:1040602/1‑140 (MQ=255)
                                   ccaATCTGCATCAGCGCCGCAGCCAACGTACCCGGCTCTTTAAACGCTTCGATACCAAAAGCAAAAGAAAGCGCGATACACAGACCACCTGCAACGACCATAGGCAGCATGTAAGAAACGCCCGTCAGCAAGTGACGGTa                                                                                                    <  1:111886/140‑1 (MQ=255)
                                     aaTCTGCATCAGCGCCGCAGCCAACGTACCCGGCTCTTTAAACGCTTCGATACCAAAAGCAAAAGAAAGCGCGATACACAGACCACCTGCAACGACCATAGGCAGCATGTAAGAAACGCCCGTCAGCAAGTGACGGTATg                                                                                                  <  2:2204866/140‑1 (MQ=255)
                                        cTGCATCAGCGCCGCAGCCAACGTACCCGGCTCTTTAAACGCTTCGATACCAAAAGCAAAAGAAAGTGCGATACACAGACCACCTGCAACGACCATAGGCAGCATGTAAGAAACGCCCGTCAGCAAGTGACGGTATgcgc                                                                                               >  2:732215/1‑140 (MQ=255)
                                        cTGCATCAGCGCCGCAGCCAACGTACCCGGCTCTTTAAACGCTTCGATACCAAAAGCAAAAGAAAGCGCGATACACAGACCACCTGCAACGACCATAGGCAGCATGTAAGAAACGCCCGTCAGCAAGTGACGGTATgcgc                                                                                               >  2:2642036/1‑140 (MQ=255)
                                         tGCATCAGCGCCGCAGCCAACGTACCCGGCTCTTTAAACGCTTCGATACCAAAAGCAAAAGAAAGTGCGATACACAGACCACCTGCAACGACCATAGGCAGCATGTAAGAAACGCCCGTCAGCAAGTGACGGTATGCGcc                                                                                              <  1:732215/140‑1 (MQ=255)
                                           cATCAGCGCCGCAGCCAACGTACCCGGCTCTTTAAACGCTTCGATACCAAAAGCAAAAGAAAGCGCGATACACAGACCACCTGCAACGACCATAGGCAGCATGTAAGAAACGCCCGTCAGCAAGTGACGGTATGCGCCTg                                                                                            >  1:2022291/1‑140 (MQ=255)
                                              cAGCGCCGCAGCCAACGTACCCGGCTCTTTAAACGCTTCGATACCAAAAGCAAAAGAAAGCGCGATACACAGACCACCTGCAACGACCATAGGCAGCATGTAAGAAACGCCCGTCAGCAAGTGACGGTATg                                                                                                  <  2:430855/131‑1 (MQ=255)
                                              cAGCGCCGCAGCCAACGTACCCGGCTCTTTAAACGCTTCGATACCAAAAGCAAAAGAAAGCGCGATACACAGACCACCTGCAACGACCATAGGCAGCATGTAAGAAACGCCCGTCAGCAAGTGACGGTATg                                                                                                  >  1:430855/1‑131 (MQ=255)
                                              cAGCGCCGCAGCCAACGTACCCGGCTCTTTAAACGCTTCGATACCAAAAGCAAAAGAAAGCGCGATACACAGACCACCTGCAACGACCATAGGCAGCATGTAAGAAACGCCCGTCAGCAAGTGACGGTATGCGCCTGCAc                                                                                         >  1:1321038/1‑140 (MQ=255)
                                              cAGCGCCGCAGCCAACGTACCCGGCTCTTTAAACGCTTCGATACCAAAAGCAAAAGAAAGCGCGATACACAGACCACCTGCAACGACCATAGGCAGCATGTAAGAAACGCCCGTCAGCAAGTGACGGTATGCGCCTGCAc                                                                                         >  1:26079/1‑140 (MQ=255)
                                               aGCGCCGCAGCCAACGTACCCGGCTCTTTAAACGCTTCGATACCAAAAGCAAAAGAAAGCGCGATACACAGACCACCTGCAACGACCATAGGCAGCATGTAAGAAACGCCCGTCAGCAAGTGACGGTATGCGCCTGCAct                                                                                        <  1:1040602/140‑1 (MQ=255)
                                                gcgcCGCAGCCAACGTACCCGGCTCTTTAAACGCTTCGATACCAAAAGCAAAAGAAAGCGCGATACACAGACCACCTGCAACGACCATAGGCAGCATGTAAGAAACGCCCGTCAGCAAGTGACGGTATGCGCCTGCActc                                                                                       <  2:1154332/140‑1 (MQ=255)
                                                gcgcCGCAGCCAACGTACCCGGCTCTTTAAACGCTTCGATACCAAAAGCAAAAGAAAGCGCGATACACAGACCACCTGCAACGACCATAGGCAGCATGTAAGAAACGCCCGTCAGCAAGTGACGGTATGCGCCTGCActc                                                                                       >  1:2057302/1‑140 (MQ=255)
                                                gcgcCGCAGCCAACGTACCCGGCTCTTTAAACGCTTCGATACCAAAAGCAAAAGAAAGCGCGATACACAGACCACCTGCAACGACCATAGGCAGCATGTAAGAAACGCCCGTCAGCAAGTGACGGTATGCGCCTGCActc                                                                                       >  2:64852/1‑140 (MQ=255)
                                                 cgccgcAGCCAACGTACCCGGCTCTTTAAACGCTTCGATACCAAAAGCAAAAGAAAGCGCGATACACAGACCACCTGCAACGACCATAGGCAGCATGTAAGAAACGCCCGTCAGCAAGTGACGGTATGCGCCTGCActct                                                                                      <  2:2489695/140‑1 (MQ=255)
                                                 cgccgcAGCCAACGTACCCGGCTCTTTAAACGCTTCGATACCAAAAGCAAAAGAAAGCGCGATACACAGACCACCTGCAACGACCATAGGCAGCATGTAAGAAACGCCCGTCAGCAAGTGACGGTATGCGCCTGCActct                                                                                      >  2:2309828/1‑140 (MQ=255)
                                                 cgccgcAGCCAACGTACCCGGATCTTTAAACGCTTCGATACCAAAAGCAAAAGAAAGCGCGATACACAGACCACCTGCAACGACCATAGGCAGCATGTAAGAAACGCCCGTCAGCAAGTGACGGTATGCGCCTGCActct                                                                                      >  2:1782869/1‑140 (MQ=255)
                                                     gcAGCCAACGTACCCGGCTCTTTAAACGCTTCGATACCAAAAGCAAAAGAAAGCGCGATACACAGACCACCTGCAACGACCATAGGCAGCATGTAAGAAACGCCTGTCAGCAAGTGACGGTATGCGCCTGCACTCtcttt                                                                                  <  1:155887/140‑1 (MQ=255)
                                                     gcAGCCAACGTACCCGGCTCTTTAAACGCTTCGATACCAAAAGCAAAAGAAAGCGCGATACACAGACCACCTGCAACGACCATAGGCAGCATGTAAGAAACGCCCGTCAGCAAGTGACGGTATGCGCCTGCACTCtcttt                                                                                  <  2:2579635/140‑1 (MQ=255)
                                                        gCCAACGTACCCGGCTCTTTAAACGCTTCGATACCAAAAGCAAAAGAAAGCGCGATACACAGACCACCTGCAACGACCATAGGCAGCATGTAAGAAACGCCCGTCAGCAAGTGACGGTATGCGCCTGCACTCtctttctt                                                                               <  1:2341211/140‑1 (MQ=255)
                                                           aaCGTACCCGGCTCTTTAAACGCTTCGATACCAAAAGCAAAAGAAAGCGCGATACACAGACCACCTGCAACGACCATAGGCAGCATGTAAGAAACGCCCGTCAGCAAGTGACGGTATGCGCCTGCACTCTCTTTCTTACt                                                                            >  1:1922509/1‑140 (MQ=255)
                                                                aCCCGGCTCTTTAAACGCTTCGATACCAAAAGCAAAAGAAAGCGCGATACACAGACCACCTGCAACGACCATAGGCAGCATGTAAGAAACGCCCGTCAGCAAGTGACGGTATGCGCCTGCACTCTCTTTCTTACTTTCAg                                                                       <  1:1349854/140‑1 (MQ=255)
                                                                 cccGGCTCTTTAAACGCTTCGATACCAAAAGCAAAAGAAAGCGCGATACACAGACCACCTGCAACGACCATAGGCAGCATGTAAGAAACGCCCGTCAGCAAGTGACGGTATGCGCCTGCACTCTCTTTCTTACTTTCAGt                                                                      >  1:1910262/1‑140 (MQ=255)
                                                                    ggCTCTTTAAACGCTTCGATACCAAAAGCAAAAGAAAGCGCGATACACAGACCACCTGCAACGACCATAGGCAGCATGTAAGAAACGCCCGTCAGCAAGTGACGGTATGCGCCTGCACTCTCTTTCTTACTTTCAGTGGt                                                                   <  2:1853107/140‑1 (MQ=255)
                                                                           tAAACGCTTCGATACCAAAAGCAAAAGAAAGCGCGATACACAGACCACCTGCAACGACCATAGGCAGCATGTAAGAAACGCCCGTCAGCAAGTGACGGTATGCGCCTGCACTCTCTTTCTTACTTTCAGTGGTCGCCGtt                                                            <  2:1028619/140‑1 (MQ=255)
                                                                           tAAACGCTTCGATACCAAAAGCAAAAGAAAGCGCGATACACAGACCACCTGCAACGACCATAGGCAGCATGTAAGAAACGCCCGTCAGCAAGTGACGGTATGCGCCTGCACTCTCTTTCTTACTTTCAGTGGTCGCCGtt                                                            <  1:1782869/140‑1 (MQ=255)
                                                                                gCTTCGATACCAAAAGCAAAAGAAAGTGCGATACACAGACCACCTGCAACGACCATAGGCAGCATGTAAGAAACGCCCGTCAGCAAGTGACGGTATGCGCCTGCACTCTCTTTCTTACTTTCAGTGGTCGCCGTTTGAGc                                                       >  1:318521/1‑140 (MQ=255)
                                                                                  ttCGATACCAAAAGCAAAAGAAAGCGCGATACACAGACCACCTGCAACGACCATAGGCAGCATGTAAGAAACGCCCGTCAGCAAGTGACGGTATGCGCCTGCACTCTCTTTCTTACTTTCAGTGGTCGCCGTTTGAGCtt                                                     >  1:2747869/1‑140 (MQ=255)
                                                                                   tCGATACCAAAAGCAAAAGAAAGCGCGATACACAGACCACCTGCAACGACCATAGGCAGCATGTAAGAAACGCCCGTCAGCAAGTGACGGTATGCGCCTGCACTCTCTTTCTTACTTTCAGTGGTCGCCGTTTGAGCttt                                                    >  1:213126/1‑140 (MQ=255)
                                                                                    cGATACCAAAAGCAAAAGAAAGCGCGATACACAGCCCACCTGCAACGACCATAGGCAGCATGTAAGAAACGCCCGTCAGCAAGTGACGGTATGCGCCTGCCCTCTCTTTCTTACTTTCAGTGGTCGCCGTTTGAGCTTTg                                                   <  2:68198/140‑1 (MQ=255)
                                                                                        aCCAAAAGCAAAAGAAAGCGCGATACACAGACCACCTGCAACGACCATAGGCAGCATGTAAGAAACGCCCGTCAGCAAGTGACGGTATGCGCCTGCACTCTCTTTCTTACTTTCAGTGGTCGCCGTTTGAGCTTTGCccg                                               <  1:2292991/140‑1 (MQ=255)
                                                                                        aCCAAAAGCAAAAGAAAGCGCGATACACAGACCACCTGCAACGACCATAGGCAGCATGTAAGAAACGCCCGTCAGCAAGTGACGGTATGCGCCTGCACTCTCTTTCTTACTTTCAGTGGTCGCCGTTTGAGCTTTGCccg                                               <  2:2787139/140‑1 (MQ=255)
                                                                                            aaagcaaaagAAAGCGCGATACACAGACCACCTGCAACGACCATAGGCAGCATGTAAGAAACGCCCGTCAGCAAGTGACGGTATGCGCCTGCACTCTCTTTCTTACTTTCAGTGGTCGCCGTTTGAGCTTTGCCCGCCgg                                           <  1:64852/140‑1 (MQ=255)
                                                                                            aaagcaaaagAAAGCGCGATACACAGACCACCTGCAACGACCATAGGCAGCATGTAAGAAACGCCCGTCAGCAAGTGACGGTATGCGCCTGCACTCTCTTTCTTACTTTCAGTGGTCGCCGTTTGAGCTTTGCCCGCCgg                                           >  2:2099815/1‑140 (MQ=255)
                                                                                              agcaaaagAAAGCGCGATACACAGACCACCTGCAACGACCATAGGCAGCATGTAAGAAACGCCCGTCAGCAAGTGACGGTATGCGCCTGCACTCTCTTTCTTACTTTCAGTGGTCGCCGTTTGAGCTTTGCCCGCCGGtt                                         >  2:2219677/1‑140 (MQ=255)
                                                                                                 aaaagAAAGCGCGATACACAGACCACCTGCAACGACCATAGGCAGCATGAAAGAAACGCCCGTAAGCAAGTGACGGTATGCGCCTGCACCCTTTTTCTTACATTCAGTGGTCGCCGTTTGAGCTTTGCCCGCCGGTTCAt                                      >  2:288209/1‑140 (MQ=255)
                                                                                                   aagaaagCGCGATACACAGACCACCTGCAACGACCATAGGCAGCATGTAAGAAACGCCCGTCAGCAAGTGACGGTATGCGCCTGCACTCTCTTTCTTACTTTCAGTGGTCGCCGTTTGAGCTTTGCCCGCCGGTTCATAc                                    >  1:11402/1‑140 (MQ=255)
                                                                                                         gcgcGATACACAGACCCCCTGCAACGCCCATAGGCAGCATGTAAGAACCGCCCGTCAGCAAGTGACGGTACGCGCCTGCACTCTCTTTCTTCCTTTCAGTGGCCGCCGTTTGAGCTTTCCCCGCCGGTTCATACGGCGtt                              <  2:2747869/140‑1 (MQ=255)
                                                                                                         gcgcGATACACAGACCACCTGCAACGACCATAGGCAGCATGTAAGAAACGCCCGTCAGCAAGTGACGGTATGCGCCTGCACTCTCTTTCTTACTTTCAGTGGTCGCCGTTTGAGCTTTGCCCGCCGGTTCATACGGCGtt                              <  1:2642036/140‑1 (MQ=255)
                                                                                                         gcgcGATACACAGACCACCTGCAACGACCATAGGCAGCATGTAAGAAACGCCCGTCAGCAAGTGACGGTATGCGCCTGCACTCTCTTTCTTACTTTCAGTGGTCGCCGTTTGAGCTTTGCCCGCCGGTTCATACGGCGtt                              >  2:145871/1‑140 (MQ=255)
                                                                                                                   caGACCACCTGCAACGACCATAGGCAGCATGTAAGAAACGCCCGTCAGCAAGTGACGGTATGCGCCTGCACTCTCTTTCTTACTTTCAGTGGTCGCCGTTTGAGCTTTGCCCGCCGGTTCATACGGCGTTGCTTCAGCaa                    <  1:2725033/140‑1 (MQ=255)
                                                                                                                    aGACCACCTGCAACGACCATAGGCAGCATGTAAGAAACGCCCGTCAGCAAGTGACGGTATGCGCCTGCACTCTCTTTCTTACTTTCAGTGGTCGCCGTTTGAGCTTTGCCCGCCGGTTCATACGGCGTTGCTTCAGCAAc                   >  2:2933282/1‑140 (MQ=255)
                                                                                                                      accaccTGCAACGACCATAGGCAGCATGTAAGAAACGCCCGTCAGCAAGTGACGGTATGCGCCTGCACTCTCTTTCTTACTTTCAGTGGTCGCCGTTTGAGCTTTGCCCGCCGGTTCATACGGCGTTGCTTCAGCAACCg                 >  1:2166226/1‑140 (MQ=255)
                                                                                                                       ccaccTGCAACGACCATAGGCAGCATGTAAGAAACGCCCGTCAGCAAGTGACGGTATGCGCCTGCACTCTCTTTCTTACTTTCAGTGGTCGCCGTTTGAGCTTTGCCCGCCGGTTCATACGGCGTTGCTTCAGCAACCGc                <  1:1646096/140‑1 (MQ=255)
                                                                                                                        caccTGCAACGACCATAGGCAGCATGTAAGAAACGCCCGTCAGCAAGTGACGGTATGCGCCTGCACTCTCTTTCTTACTTTCAGTGGTCGCCGTTTGAGCTTTGCCCGCCGGTTCATACGGCGTTGCTTCAGCAACCGCt               >  1:2835903/1‑140 (MQ=255)
                                                                                                                         accTGCAACGACCATAGGCAGCATGTAAGAAACGCCCGTCAGCAAGTGACGGTATGCGCCTGCACTCTCTTTCTTACTTTCAGTGGTCGCCGTTTGAGCTTTGCCCGCCGGTTCATACGGCGTTGCTTCAGCAACCGCtt              >  1:1290846/1‑140 (MQ=255)
                                                                                                                         accTGCAACGACCATAGGCAGCATGTAAGAAACGCCCGTCAGCAAGTGACGGTATGCGCCTGCACTCTCTTTCTTACTTTCAGTGGTCGCCGTTTGAGCTTTGCCCGCCGGTTCATACGGCGTTGCTTCAGCAACCGCtt              >  2:505146/1‑140 (MQ=255)
                                                                                                                         accTGCAACGACCATAGGCAGCATGTAAGAAACGCCCGTCAGCAAGTGACGGTATGCGCCTGCACTCTCTTTCTTACTTTCAGTGGTCGCCGTTTGAGCTTTGCCCGCCGGTTCATACGGCGTTGCTTCAGCAACCGCtt              >  2:308894/1‑140 (MQ=255)
                                                                                                                         accTGCAACGACCATAGGCAGCATGTAAGAAACGCCCGTCAGCAAGTGACGGTATGCGCCTGCACTCTCTTTCTTACTTTCAGTGGTCGCCGTTTGAGCTTTGCCCGCCGGTTCATACGGCGTTGCTTCAGCAACCGCtt              >  2:1998863/1‑140 (MQ=255)
                                                                                                                                  gACCATAGGCAGCATGTAAGAAACGCCCGTCAGCAAGTGACGGTATGCGCCTGCACTCTCTTTCTTACTTTCAGTGGTCGCCGTTTGAGCTTTGCCCGCCGGTTCATACGGCGTTGCTTCAGCAACCGCTTTATCCAGtt     >  2:1084234/1‑140 (MQ=255)
                                                                                                                                  gACCATAGGCAGCATGTAAGAAACGCCCGTCAGCAAGTGACGGTATGCGCCTGCACTCTCTTTCTTACTTTCAGTGGTCGCCGTTTGAGCTTTGCCCGCCGGTTCATACGGCGTTGCTTCAGCAACCGCTTTATCCAGtt     >  2:1768481/1‑140 (MQ=255)
                                                                                                                                    ccATAGGCAGCATGTAAGAAACGCCCGTCAGCAAGTGACGGTATGCGCCTGCACTCTCTTTCTTACTTTCAGTGGTCGCCGTTTGAGCTTTGCCCGCCGGTTCATACGGCGTTGCTTCAGCAACCGCTTTATCCAGTTcc   >  2:1264598/1‑140 (MQ=255)
                                                                                                                                        |                                                                                                                                        
GTACCGGCACCATCAGCGCAAAGGCTGAACCACCACCAATCTGCATCAGCGCCGCAGCCAACGTACCCGGCTCTTTAAACGCTTCGATACCAAAAGCAAAAGAAAGCGCGATACACAGACCACCTGCAACGACCATCGGCAGCATGTAAGAAACGCCCGTCAGCAAGTGACGGTATGCGCCTGCACTCTCTTTCTTACTTTCAGTGGTCGCCGTTTGAGCTTTGCCCGCCGGTTCATACGGCGTTGCTTCAGCAACCGCTTTATCCAGTTCCT  >  NC_000913/2260558‑2260830

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑