Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 415,660 G→T L32M (CTG→ATG)  sbcC ← ATP dependent, structure specific DNA nuclease ‑ SbcC subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913415,6600GT97.8% 487.3 / 0.5 138L32M (CTG→ATG) sbcCATP dependent, structure specific DNA nuclease ‑ SbcC subunit
Reads supporting (aligned to +/- strand):  ref base G (1/2);  new base T (71/64);  total (72/66)
Fisher's exact test for biased strand distribution p-value = 6.06e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 139 total aligned reads displayed.

GGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGAGAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAACAGCCCGTTGCTGGCGAACGGCTCGCGGGTGAAATCAATCTTCCATTCGCCTTTTAATGAGTTCAGGTTTTTCAGGCGCAGGCTGAGAATTTTCATGCTTCGTGTTCTCCGGCGAGGGTATGCAACGTCGTGGTGAAAA  >  NC_000913/415526‑415796
                                                                                                                                      |                                                                                                                                        
ggTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGAGAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAACATCCCGt                                                                                                                                     >  2:2167273/1‑140 (MQ=255)
  tATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGAGAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAACATCCCGTTg                                                                                                                                   >  1:839504/1‑140 (MQ=255)
         ggTCATGAGATCATTTTGCGATTGTGAAACGTTAGAGAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAACATCCCGTTGCTGGCGa                                                                                                                            >  1:841737/1‑140 (MQ=255)
         ggTCATGAGATCATTTTGCGATTGTGAAACGTTAGAGAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAACATCCCGTTGCTGGCGa                                                                                                                            >  1:354464/1‑140 (MQ=255)
            cATGAGATCATTTTGCGATTGTGAAACGTTAGAGAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAACATCCCGTTGCTGGCGAACg                                                                                                                         >  1:1079307/1‑140 (MQ=255)
            cATGAGATCATTTTGCGATTGTGAAACGTTAGAGAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAACATCCCGTTGCTGGCGAACg                                                                                                                         >  1:1772280/1‑140 (MQ=255)
             aTGAGATCATTTTGCGATTGTGAAACGTTAGAGAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAACATCCCGTTGCTGGCGAACgg                                                                                                                        <  1:179374/140‑1 (MQ=255)
              tGAGATCATTTTGCGATTGTGAAACGTTAGAGAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAACATCCCGTTGCTGGCGAACGGc                                                                                                                       >  1:1958620/1‑140 (MQ=255)
              tGAGATCATTTTGCGATTGTGAAACGTTAGAGAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAACATCCCGTTGCTGGCGAACGGc                                                                                                                       >  1:1918318/1‑140 (MQ=255)
               gagaTCATTTTGCGATTGTGAAACGTTAGAGAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAACATCCCGTTGCTGGCGAACGGCt                                                                                                                      <  1:60531/140‑1 (MQ=255)
                agaTCATTTTGCGATTGTGAAACGTTAGAGAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAACATCCCGTTGCTGGCGAACGGCTc                                                                                                                     <  1:2128606/140‑1 (MQ=255)
                 gaTCATTTTGCGATTGTGAAACGTTAGAGAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAACATCCCGTTGCTGGCGAACGGCTcg                                                                                                                    <  2:1630846/140‑1 (MQ=255)
                 gaTCATTTTGCGATTGTGAAACGTTAGAGAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAACATCCCGTTGCTGGCGAACGGCTcg                                                                                                                    <  2:354464/140‑1 (MQ=255)
                 gaTCATTTTGCGATTGTGAAACGTTAGAGAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAACATCCCGTTGCTGGCGAACGGCTcg                                                                                                                    <  2:939634/140‑1 (MQ=255)
                 gaTCATTTTGCGATTGTGAAACGTTAGAGAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAACATCCCGTTGCTGGCGAACGGCTcg                                                                                                                    <  2:2539222/140‑1 (MQ=255)
                  aTCATTTTGCGATTGTGAAACGTTAGAGAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAACATCCCGTTGCTGGCGAACGGCTcgc                                                                                                                   >  1:742127/1‑140 (MQ=255)
                    cATTTTGCGATTGTGAAACGTTAGAGAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAACATCCCGTTGCTGGCGAACGGCTCGCgg                                                                                                                 >  2:1729689/1‑140 (MQ=255)
                      ttttGCGATTGTGAAACGTTAGAGAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAACATCCCGTTGCTGGCGAACGGCTCGCGGGt                                                                                                               <  2:964181/140‑1 (MQ=255)
                      ttttGCGATTGTGAAACGTTAGAGAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAACATCCCGTTGCTGGCGAACGGCTCGCGGGt                                                                                                               <  1:1889156/140‑1 (MQ=255)
                        ttGCGATTGTGAAACGTTAGAGAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAACATCCCGTTGCTGGCGAACGGCTCGCGGGTGa                                                                                                             <  2:968278/140‑1 (MQ=255)
                         tGCGATTGTGAAACGTTAGAGAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAACATCCCGTTGCTGGCGAACGGCTCGCGGGTGaa                                                                                                            >  2:2813484/1‑140 (MQ=255)
                          gCGATTGTGAAACGTTAGAGAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAACATCCCGTTGCTGGCGAACGGCTCGCGGGTGaaa                                                                                                           >  1:1000450/1‑140 (MQ=255)
                           cGATTGTGAAACGTTAGAGAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAACATCCCGTTGCTGGCGAACGGCTcgc                                                                                                                   >  2:572632/1‑131 (MQ=255)
                           cGATTGTGAAACGTTAGAGAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAACATCCCGTTGCTGGCGAACGGCTcgc                                                                                                                   <  1:572632/131‑1 (MQ=255)
                           cGATTGTGAAACGTTAGAGAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAACATCCCGTTGCTGGCGAACGGCTCGCGGGTGAaat                                                                                                          >  1:880328/1‑140 (MQ=255)
                                gtgAAACGTTAGAGAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAACATCCCGTTGCTGGCGAACGGCTCGCGGGTGAaatcaatc                                                                                                     >  2:1328325/1‑140 (MQ=255)
                                      cGTTAGAGAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAACATCCCGTTGCTGGCGAACGGCTCGCGGGTGAAATCAATCTTCCAt                                                                                               <  1:1729689/140‑1 (MQ=255)
                                        ttAGAGAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAACATCCCGTTGCTGGCGAACGGCTCGCGGGTGAAATCAATCTTCCATTc                                                                                             <  2:1017466/140‑1 (MQ=255)
                                        ttAGAGAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAACAGCCCGTTGCTGGCGAACGGCTCGCGGGTGAAATCAATCTTCCATTc                                                                                             >  1:1172241/1‑140 (MQ=255)
                                        ttAGAGAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAACAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAACATCCCGTTGCTGGCGAACGGCTCGCGGGTGAAATCAATCTTCCATTc                                                                                             >  1:2613689/1‑140 (MQ=255)
                                        ttAGAGAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAACAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAACATCCCGTTGCTGGCGAACGGCTCGCGGGTGAAATCAATCTTCCATTc                                                                                             <  2:2613689/140‑1 (MQ=255)
                                                aCGCGGAGTTTCGTGATACATCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAACATCCCGTTGCTGGCGAACGGCTCGCGGGTGAAATCAATCTTCCATTCGCCTTTTa                                                                                     >  2:1053117/1‑140 (MQ=255)
                                                 cgcgGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAACATCCCGTTGCTGGCGAACGGCTCGCGGGTGAAATCAATCTTCCATTCGCCTTTTaa                                                                                    >  2:1240051/1‑140 (MQ=255)
                                                 cgcgGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAACATCCCGTTGCTGGCGAACGGCTCGCGGGTGAAATCAATCTTCCATTCGCCTTTTaa                                                                                    <  2:2147990/140‑1 (MQ=255)
                                                  gcgGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAACATCCCGTTGCTGGCGAACGGCTCGCGGGTGAAATCAATCTTCCATTCGCCTTTTAAt                                                                                   <  1:567076/140‑1 (MQ=255)
                                                  gcgGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAACATCCCGTTGCTGGCGAACGGCTCGCGGGTGAAATCAATCTTCCATTCGCCTTTTAAt                                                                                   >  1:1576459/1‑140 (MQ=255)
                                                  gcgGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAACATCCCGTTGCTGGCGAACGGCTCGCGGGTGAAATCAATCTTCCATTCGCCTTTTAAt                                                                                   >  1:1531233/1‑140 (MQ=255)
                                                   cgGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAACATCCCGTTGCTGGCGAACGGCTCGCGGGTGAAATCAATCTTCCAtt                                                                                              >  2:1194574/1‑128 (MQ=255)
                                                   cgGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAACATCCCGTTGCTGGCGAACGGCTCGCGGGTGAAATCAATCTTCCAtt                                                                                              <  1:1194574/128‑1 (MQ=255)
                                                     gAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAACATCCCGTTGCTGGCGAACGGCTCGCGGGTGAAATCAATCTTCCATTCGCCTTTTAATGAg                                                                                >  2:34994/1‑140 (MQ=255)
                                                      aGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAACATCCCGTTGCTGGCGAACGGCTCGCGGGTGAAATCAATCTTCCATTCGCCTTTTAATGAGt                                                                               <  1:282175/140‑1 (MQ=255)
                                                       gTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAACATCCCGTTGCTGGCGAACGGCTCGCGGGTGAAATCAATCTTCCATTCGCCTTTTAATGAGtt                                                                              <  1:2199417/140‑1 (MQ=255)
                                                            gTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAACATCCCGTTGCTGGCGAACGGCTCGCGGGTGAAATCAATCTTCCATTCGCCTTTTAATGAGTTCAGGt                                                                         <  2:2319078/140‑1 (MQ=255)
                                                             tGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAACATCCCGTTGCTGGCGAACGGCTCGCGGGTGAAATCAATCTTCCATTCGCCTTTTAATGAGTTCAGGtt                                                                        >  2:751220/1‑140 (MQ=255)
                                                                tACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCGCCTGTTGGGCCGGTAATAGCAAACATCCCGTTGCTGGCGAACGGCTCGCGGGTGAAATCAATCTTCCATTCGCCTTTTAATGAGTTCAGGttttt                                                                     <  2:965969/140‑1 (MQ=255)
                                                                  cAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAACATCCCGTTGCTGGCGAACGGCTCGCGGGTGAAATCAATCTTCCATTCGCCTTTTAATGAGTTCAGGTTTTTCa                                                                   >  2:873864/1‑140 (MQ=255)
                                                                  cAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAACATCCCGTTGCTGGCGAACGGCTCGCGGGTGAAATCAATCTTCCATTCGCCTTTTAATGAGTTCAGGTTTTTCa                                                                   >  1:2929447/1‑140 (MQ=255)
                                                                  cAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAACATCCCGTTGCTGGCGAACGGCTCGCGGGTGAAATCAATCTTCCATTCGCCTTTTAATGAGTTCAGGTTTTTCa                                                                   >  2:411520/1‑140 (MQ=255)
                                                                     cgcCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAACATCCCGTTGCTGGCGAACGGCTCGCGGGTGAAATCAATCTTCCATTCGCCTTTTAATGAGTTCAGGTTTTTCAGgc                                                                >  1:141184/1‑140 (MQ=255)
                                                                     cgcCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAACATCCCGTTGCTGGCGAACGGCTCGCGGGTGAAATCAATCTTCCATTCGCCTTTTAATGAGTTCAGGTTTTTCAGgc                                                                >  1:1445786/1‑140 (MQ=255)
                                                                     cgcCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAACATCCCGTTGCTGGCGAACGGCTCGCGGGTGAAATCAATCTTCCATTCGCCTTTTAATGAGTTCAGGTTTTTCAGgc                                                                >  2:2417404/1‑140 (MQ=255)
                                                                     cgcCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAACATCCCGTTGCTGGCGAACGGCTCGCGGGTGAAATCAATCTTCCATTCGCCTTTTAATGAGTTCAGGTTTTTCAGgc                                                                >  1:975939/1‑140 (MQ=255)
                                                                     cgcCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAACATCCCGTTGCTGGCGAACGGCTCGCGGGTGAAATCAATCTTCCATTCGCCTTTTAATGAGTTCAGGTTTTTCAGgc                                                                >  1:2480821/1‑140 (MQ=255)
                                                                     cgcCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAACATCCCGTTGCTGGCGAACGGCTCGCGGGTGAAATCAATCTTCCATTCGCCTTTTAATGAGTTCAGGTTTTTCAGgc                                                                >  2:57855/1‑140 (MQ=255)
                                                                      gcCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAACATCCCGTTGCTGGCGAACGGCTCGCGGGTGAAATCAATCTTCCATTCGCCTTTTAATGAGTTCAGGTTTTTCAGgcg                                                               <  2:1958620/140‑1 (MQ=255)
                                                                      gcCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAACATCCCGTTGCTGGCGAACGGCTCGCGGGTGAAATCAATCTTCCATTCGCCTTTTAATGAGTTCAGGTTTTTCAGgcg                                                               <  1:1053117/140‑1 (MQ=255)
                                                                      gcCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAACATCCCGTTGCTGGCGAACGGCTCGCGGGTGAAATCAATCTTCCATTCGCCTTTTAATGAGTTCAGGTTTTTCAGgcg                                                               <  1:2658743/140‑1 (MQ=255)
                                                                        cAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAACATCCCGTTGCTGGCGAACGGCTCGCGGGTGAAATCAATCTTCCATTCGCCTTTTAATGAGTTCAGGTTTTTCAGGCGCa                                                             >  2:2066114/1‑140 (MQ=255)
                                                                          gACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAACATCCCGTTGCTGGCGAACGGCTCGCGGGTGAAATCAATCTTCCATTCGCCTTTTAATGAGTTCAGGTTTTTCAGGCGCAgg                                                           >  2:1083206/1‑140 (MQ=255)
                                                                          gACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAACATCCCGTTGCTGGCGAACGGCTCGCGGGTGAAATCAATCTTCCATTCGCCTTTTAATGAGTTCAGGTTTTTCAGGCGCAgg                                                           >  2:2187311/1‑140 (MQ=255)
                                                                          gACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAACATCCCGTTGCTGGCGAACGGCTCGCGGGTGAAATCAATCTTCCATTCGCCTTTTAATGAGTTCAGGTTTTTCAGGCGCAg                                                            <  1:2845984/139‑1 (MQ=255)
                                                                          gACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAACATCCCGTTGCTGGCGAACGGCTCGCGGGTGAAATCAATCTTCCATTCGCCTTTTAATGAGTTCAGGTTTTTCAGGCGCAg                                                            >  2:2845984/1‑139 (MQ=255)
                                                                               aTGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAACATCCCGTTGCTGGCGAACGGCTCGCGGGTGAAATCAATCTTCCATTCGCCTTTTAATGAGTTCAGGTTTTTCAGGCGCAGGCTgag                                                      <  2:1772280/140‑1 (MQ=255)
                                                                               aTGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAACATCCCGTTGCTGGCGAACGGCTCGCGGGTGAAATCAATCTTCCATTCGCCTTTTAATGAGTTCAGGTTTTTCAGGCGCAGGCTgag                                                      <  1:873864/140‑1 (MQ=255)
                                                                               aTGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAACATCCCGTTGCTGGCGAACGGCTCGCGGGTGAAATCAATCTTCCATTCGCCTTTTAATGAGTTCAGGTTTTTCAGGCGCAGGCTgag                                                      >  1:790074/1‑140 (MQ=255)
                                                                               aTGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAACATCCCGTTGCTGGCGAACGGCTCGCGGGTGAAATCAATCTTCCATTCGCCTTTTAATGAGTTCACGTTTTTCAGGCGCAGGCTgag                                                      <  1:638663/140‑1 (MQ=255)
                                                                                 ggCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAACATCCCGTTGCTGGCGAACGGCTCGCGGGTGAAATCAATCTTCCATTCGCCTTTTAATGAGTTCAGGTTTTTCAGGCGCAGGCTGAGaa                                                    <  1:34994/140‑1 (MQ=255)
                                                                                    gTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAACATCCCGTTGCTGGCGAACGGCTCGCGGGTGAAATCAATCTTCCATTCGCCTTTTAATGAGTTCAGGTTTTTCAGGCGCAGGCTGAGAAttt                                                 >  1:2718604/1‑140 (MQ=255)
                                                                                    gTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAACATCCCGTTGCTGGCGAACGGCTCGCGGGTGAAATCAATCTTCCATTCGCCTTTTAATGAGTTCAGGTTTTTCAGGCGCAGGCTGAGAAttt                                                 >  1:71878/1‑140 (MQ=255)
                                                                                     tCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAACATCCCGTTGCTGGCGAACGGCTCGCGGGTGAAATCAATCTTCCATTCGCCTTTTAATGAGTTCAGGTTTTTCAGGCGCAGGCTGAGAAtttt                                                >  1:930774/1‑140 (MQ=255)
                                                                                        agcagGGTGGTTTTCTCCGCACCTGTTGGGCTGGTAATAGCAAACATCCCGTTGCTGGCGAACGGCTCGCGGGTGAAATCAATCTTCCATTCGCCTTTTAATGAGTTCAGCTTTTTCAGGCGCAGGCTGAGAATTTTCAt                                             <  2:2808562/140‑1 (MQ=255)
                                                                                        agcagGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAACATCCCGTTGCTGGCGAACGGCTCGCGGGTGAAATCAATCTTCCATTCGCCTTTTAATGAGTTCAGGTTTTTCAGGCGCAGGCTGAGAATTTTCAt                                             <  2:233768/140‑1 (MQ=255)
                                                                                        agcagGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAACATCCCGTTGCTGGCGAACGGCTCGCGGGTGAAATCAATCTTCCATTCGCCTTTTAATGAGTTCAGGTTTTTCAGGCGCAGGCTGAGAATTTTCAt                                             <  1:2167273/140‑1 (MQ=255)
                                                                                          cagGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAACATCCCGTTGCTGGCGAACGGCTCGCGGGTGAAATCAATCTTCCATTCGCCTTTTAATGAGTTCAGGTTTTTCAGGCGCAGGCTGAGAATTTTCATGc                                           <  1:2417404/140‑1 (MQ=255)
                                                                                          cagGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAACAGCCCGTTGCTGGCGAACGGCTCGCGGGTGAAATCAATCTTCCATTCGCCTTTTAATGAGTTCAGGTTTTTCAGGCGCAGGCTGAGAATTTTCATGc                                           <  1:1265177/140‑1 (MQ=255)
                                                                                               tggtTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAACATCCCGTTGCTGGCGAACGGCTCGCGGGTGAAATCAATCTTCCATTCGCCTTTTAATGAGTTCAGGTTTTTCAGGCGCAGGCTGAGAATTTTCATGCTTCgt                                      <  1:1785897/140‑1 (MQ=255)
                                                                                               tggcTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAACATCCCGTTGCTGGCGAACGGCTCGCGGGTGAAATCAATCTTCCATTCGCCTTTTAATGAGGTCAGGTTTTTCAGGCGCAGGCTGAGAATTTTCATGCTTCgt                                      >  2:2882119/1‑140 (MQ=255)
                                                                                                ggtTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAACATCCCGTTGCTGGCGAACGGCTCGCGGGTGAAATCAATCTTCCATTCGCCTTTTAATGAGTTCAGGTTTTTCAGGCGCAGGCTGAGAATTTTCATGCTTCgtg                                     >  2:1928532/1‑140 (MQ=255)
                                                                                                   tttCCCCGCACCTGTTGGGCCGGTAATAGCAAACATCCCGTTGCTGGCGAACGGCTCGCGGGTGAAATCAATCTTCCATTCGCCTTTTAATGAGTTCAGGTTTTTCAGGCGCAGGCTGAGAATTTTCATGCTTCGTGTtc                                  <  1:63466/140‑1 (MQ=255)
                                                                                                    ttCCCCGCACCTGTTGGGCCGGTAATAGCAAACATCCCGTTGCTGGCGAACGGCTCGCGGGTGAAATCAATCTTCCATTCGCCTTTTAATGAGTTCAGGTTTTTCAGGCGCAGGCTGAGAATTTTCATGCTTCGTGTtct                                 >  1:749011/1‑140 (MQ=255)
                                                                                                    ttCCCCGCACCTGTTGGGCCGGTAATAGCAAACATCCCGTTGCTGGCGAACGGCTCGCGGGTGAAATCAATCTTCCATTCGCCTTTTAATGAGTTCAGGTTTTTCAGGCGCAGGCTGAGAATTTTCATGCTTCGTGTtct                                 <  2:2480821/140‑1 (MQ=255)
                                                                                                       cccGCACCTGTTGGGCCGGTAATAGCAAACATCCCGTTGCTGGCGAACGGCTCGCGGGTGAAATCAATCTTCCATTCGCCTTTTAATGAGTTCAGGTTTTTCAGGCGCAGGCTGGGAATTTTCATGCTTCGTGTTCTCCg                              >  1:945591/1‑140 (MQ=255)
                                                                                                       cccGCACCTGTTGGGCCGGTAATAGCAAACATCCCGTTGCTGGCGAACGGCTCGCGGGTGAAATCAATCTTCCATTCGCCTTTTAATGAGTTCAGGTTTTTCAGGCGCAGGCTGAGAATTTTCATGCTTCGTGTTCTCCg                              >  2:731424/1‑140 (MQ=255)
                                                                                                        ccGCACCTGTTGGGCCGGTAATAGCAAACATCCCGTTGCTGGCGAACGGCTCGCGGGTGAAATCAATCTTCCATTCGCCTTTTAATGAGTTCAGGTTTTTCAGGCGCAGGCTGAGAATTTTCATGCTTCGTGTTCTCCgg                             <  2:2718604/140‑1 (MQ=255)
                                                                                                        ccGCACCTGTTGGGCCGGTAATAGCAAACATCCCGTTGCTGGCGAACGGCTCGCGGGTGAAATCAATCTTCCATTCGCCTTTTAATGAGTTCAGGTTTTTCAGGCGCAGGCTGAGAATTTTCATGCTTCGTGTTCTCCgg                             >  1:2114643/1‑140 (MQ=255)
                                                                                                        ccGCACCTGTTGGGCCGGTAATAGCAAACATCCCGTTGCTGGCGAACGGCTCGCGGGTGAAATCAATCTTCCATTCGCCTTTTAATGAGTTCAGGTTTTTCAGGCGCAGGCTGAGAATTTTCATGCTTCGTGTTCTCCgg                             <  2:71878/140‑1 (MQ=255)
                                                                                                        cagCACCTGTTGGGCCGGTAATAGCAAACATCCCGTTGCTGGCGAACGGCTCGCGGGTGAAATCAATCTTCCATTCGCCTTTTAATGAGTTCAGGTTTTTCAGGCGCAGGCTGAGAATTTTCATGCTTCGTGTTCTCCgg                             <  2:974687/138‑1 (MQ=255)
                                                                                                         cGCACCTGTTGGGCCGGTAATAGCAAACATCCCGTTGCTGGCGAACGGCTCGCGGGTGAAATCAATCTTCCATTCGCCTTTTAATGAGTTCAGGTTTTTCAGGCGCAGGCTGAGAATTTTCATGCTTCGTGTTCTCCGGc                            <  2:763475/140‑1 (MQ=255)
                                                                                                           cACCTGTTGGGCCGGTAATAGCAAACATCCCGTTGCTGGCGAACGGCTCGCGGGTGAAATCAATCTTCCATTCGCCTTTTAATGAGTTCAGGTTTTTCAGGCGCAGGCTGAGAATTTTCATGCt                                          <  2:1144152/124‑1 (MQ=255)
                                                                                                           cACCTGTTGGGCCGGTAATAGCAAACATCCCGTTGCTGGCGAACGGCTCGCGGGTGAAATCAATCTTCCATTCGCCTTTTAATGAGTTCAGGTTTTTCAGGCGCAGGCTGAGAATTTTCATGCTTCGTGTTCTCCGGCGa                          <  2:2929447/140‑1 (MQ=255)
                                                                                                               tGTTGGGCCGGTAATAGCAAACATCCCGTTGCTGGCGAACGGCTCGCGGGTGAAATCAATCTTCCATTCGCCTTTTAATGAGTTCAGGTTTTTCAGGCGCAGGCTGAGAATTTTCATGCTTCGTGTTCTCCGGCGAGGGt                      <  1:1693770/140‑1 (MQ=255)
                                                                                                                        ggTAATAGCAAACATCCCGTTGCTGGCGAACGGCTCGCGGGTGAAATCAATCTTCCATTCGCCTTTTAATGAGTTCAGGTTTTTCAGGCGCAGGCTGAGAATTTTCATGCTTCGTGTTCTCCGGCGAGGGTATGCAAcgt             >  2:77935/1‑140 (MQ=255)
                                                                                                                         gTAATAGCAAACATCCCGTTGCTGGCGAACGGCTCGCGGGTGAAATCAATCTTCCATTCGCCTTTTAATGAGTTCAGGTTTTTCAGGCGCAGGCTGAGAATTTTCATGCTTCGTGTTCTCCGGCGAGGGTATGCAAcgtc            >  2:1749402/1‑140 (MQ=255)
                                                                                                                          tAATAGCAAACATCCCGTTGCTGGCGAACGGCTCGCGGGTGAAATCAATCTTCCATTCGCCTTTTAATGAGTTCAGGTTTTTCAGGCGCAGGCTGAGAATTTTCATGCTTCGTGTTCTCCGGCGa                          >  1:2198402/1‑125 (MQ=255)
                                                                                                                          tAATAGCAAACATCCCGTTGCTGGCGAACGGCTCGCGGGTGAAATCAATCTTCCATTCGCCTTTTAATGAGTTCAGGTTTTTCAGGCGCAGGCTGAGAATTTTCATGCTTCGTGTTCTCCGGCGa                          <  2:2198402/125‑1 (MQ=255)
                                                                                                                          tAATAGCAAACATCCCGTTGCTGGCGAACGGCTCGCGGGTGAAATCAATCTTCCATTCGCCTTTTAATGAGTTCAGGTTTTTCAGGCGCAGGCTGAGAATTTTCATGCTTCGTGTTCTCCGGCGAGGGTATGCAAcgtcg           <  1:1240051/140‑1 (MQ=255)
                                                                                                                           aaTAGCAAACATCCCGTTGCTGGCGAACGGCTCGCGGGTGAAATCAATCTTCCATTCGCCTTTTAATGAGTTCAGGTTTTTCAGGCGCAGGCTGAGAATTTTCATGCTTCGTGTTCTCCGGCGAGGGTATGCAAcgtcgt          >  2:2481986/1‑140 (MQ=255)
                                                                                                                            aTAGCAAACATCCCGTTGCTGGCGAACGGCTCGCGGGTGAAATCAATCTTCCATTCGCCTTTTAATGAGTTCAGGTTTTTCAGGCGCAGGCTGAGAATTTTCATGCTTCGTGTTCTCCGGCGAGGGTATGCAACGTCgtg         <  2:880468/140‑1 (MQ=255)
                                                                                                                              aGCAAACATCCCGTTGCTGGCGAACGGCTCGCGGGTGAAATCAATCTTCCATTCGCCTTTTAATGAGTTCAGGTTTTTCAGGCGCAGGCTGAGAATTTTCATGCTTCGTGTTCTCCGGCGAGGGTATGCAACGTCgtggt       >  2:2154000/1‑140 (MQ=255)
                                                                                                                                   aCATCCCGTTGCTGGCGAACGGCTCGCGGGTGAAATCAATCTTCCATTCGCCTTTTGATGAGTTCAGGATTTTCAGGCACAGGCTGCGAATTTTCCTGCTTCGTGTTCTCCGGCGAGGGCAAGGAACGTCGTGGTGaaaa  >  2:1650781/1‑140 (MQ=255)
                                                                                                                                      |                                                                                                                                        
GGTATCGCGGGTCATGAGATCATTTTGCGATTGTGAAACGTTAGAGAGACGCGGAGTTTCGTGATACAGCGCCAGACAAATGGCGTCCAGCAGGGTGGTTTTCCCCGCACCTGTTGGGCCGGTAATAGCAAACAGCCCGTTGCTGGCGAACGGCTCGCGGGTGAAATCAATCTTCCATTCGCCTTTTAATGAGTTCAGGTTTTTCAGGCGCAGGCTGAGAATTTTCATGCTTCGTGTTCTCCGGCGAGGGTATGCAACGTCGTGGTGAAAA  >  NC_000913/415526‑415796

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑