BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	561,422	G→T	D576Y (GAT→TAT)	sfmD →	putative fimbrial usher protein SfmD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	561,422	0	G	T	100.0%	702.9 / NA	198	D576Y (GAT→TAT)	sfmD	putative fimbrial usher protein SfmD
Reads supporting (aligned to +/- strand): ref base G (0/0); new base T (115/83); total (115/83)

Only 100 of 199 total aligned reads displayed.

TTTATATTTCTGGTACCCATCAAAAATACTGGAATACTTCGGATTCAGATACGTGGTATCAGGTGGGGTATACCAGCAGCTGGGTTGGCATCAGTTATTCGCTCTCATTTTCGTGGAATGAATCTGTAGGGAT‑‑CCCCGATAACGAACGTATTGTCGGACTTAATGTTTCAGTGCCTTTCAATGTTTTGACCAAACGTCGCTACACCCGGGAAAATGCGCTCGACCGCGCTTATGCCTCCTTTAACGCCAACCGTAACAGCAACGGGCAAAATAG  >  NC_000913/561285‑561558

tttATATTTCTGGTACCCATCAAAAATACTGGAATACTTCGGATTCAGATACGTGGTATCAGGTGGGGTATACCAGCAGCTGGGTTGGCATCAGTTATTCGCTCTCATTTTCGTGGAATGAATCTGTAGGGAT‑‑CCCCTAt                                                                                                                                        >  1:1035689/1‑140 (MQ=255)

  tataTTTCTGGTACCCATCAAAAATACTGGAATACTTCGGATTCAGATACGTGGTATCAGGTGGGGTATACCAGCAGCTGGGTTGGCATCAGTTATTCGCTCTCATTTTCGTGGAATGAATCTGTAGGGAT‑‑CCCCTATaa                                                                                                                                      <  1:2538043/140‑1 (MQ=255)

       ttCTGGTACCCATCAAAAATACTGGAATACTTCGGATTCAGATACGTGGTATCAGGTGGGGTATACCAGCAGCTGGGTTGGCATCAGTTATTCGCTCTCATTTTCGTGGAATGAATCTGTAGGGAT‑‑CCCCTATaacctgt                                                                                                                                 <  1:848983/140‑5 (MQ=255)

         cTGGTACCCATCAAAAATACTGGAATACTTCGGATTCAGATACGTGGTATCAGGTGGGGTATACCAGCAGCTGGGTTGGCATCAGTTATTCGCTCTCATTTTCGTGGAATGAATCTGTAGGGAT‑‑CCCCTATaa                                                                                                                                      >  1:1536946/1‑133 (MQ=255)

          tGGTACCCATCAAAAATACTGGAATACTTCGGATTCAGATACGTGGTATCAGGTGGGGTATACCAGCAGCTGGGTTGGCATCAGTTATTCGCTCTCATTTTCGTGGAATGAATCTGTAGGGAT‑‑CCCCTATAACGAACGTa                                                                                                                              <  2:669956/140‑1 (MQ=255)

            gTACCCATCAAAAATACTGGAATACTTCGGATTCAGATACGTGGTATCAGGTGGGGTATACCAGCAGCTGGGTTGGCATCAGTTATTCGCTCTCATTTTCGTGGAATGAATCTGTAGGGAT‑‑CCCCTATAACGAACGTAtt                                                                                                                            >  1:369318/1‑140 (MQ=255)

              aCCCATCAAAAATACTGGAATACTTCGGATTCAGATACGTGGTATCAGGTGGGGTATACCAGCAGCTGGGTTGGCATCAGTTATTCGCTCTCATTTTCGTGGAATGAATCTGTAGGGAT‑‑CCCCTATAACGAACGTATTGt                                                                                                                          >  2:2290125/1‑140 (MQ=255)

              aCCCATCAAAAATACTGGAATACTTCGGATTCAGATACGTGGTATCAGGTGGGGTATACCAGCAGCTGGGTTGGCATCAGTTATTCGCTCTCATTTTCGTGGAATGAATCTGTAGGGAT‑‑CCCCTATAACGAACGTATTGt                                                                                                                          <  2:1556139/140‑1 (MQ=255)

               cccATCAAAAATACTGGAATACTTCGGATTCAGATACG‑GGTATCAGGTGGGGTATACCAGCAGCTGGGTTGGCATCAGTTATTCGCTCTCATTTTCGTGGAATGAATCTGTAGGGAT‑‑CCCCTATAACGAACGTATTGTCg                                                                                                                        >  1:2918487/1‑140 (MQ=255)

                ccATCAAAAATACTGGAATACTTCGGATTCAGATACGTGGTATCAGGTGGGGTATACCAGCAGCTGGGTTGGCATCAGTTATTCGCTCTCATTTTCGTGGAATGAATCTGTAGGGAT‑‑CCCCTATAACGAACGTATTGTCg                                                                                                                        <  1:1115184/140‑1 (MQ=255)

                ccATCAAAAATACTGGAATACTTCGGATTCAGATACGTGGTATCAGGTGGGGTATACCAGCAGCTGGGTTGGCATCAGTTATTCGCTCTCATTTTCGTGGAATGAATCTGTAGGGAT‑‑CCCCTATAACGAACGTATTGTCg                                                                                                                        >  1:131404/1‑140 (MQ=255)

                  aTCAAAAATACTGGAATACTTCGGATTCAGATACGTGGTATCAGGTGGGGTATACCAGCAGCTGGGTTGGCATCAGTTATTCGCTCTCATTTTCGTGGAATGAATCTGTAGGGAT‑‑CCCCTATAACGAACGTATTGTCGGa                                                                                                                      <  1:2480926/140‑1 (MQ=255)

                  aTCAAAAATACTGGAATACTTCGGATTCAGATACGTGGTATCAGGTGGGGTATACCAGCAGCTGGGTTGGCATCAGTTATTCGCTCTCATTTTCGTGGAATGAATCTGTAGGGAT‑‑CCCCTATAACGAACGTATTGTCGGa                                                                                                                      <  2:876278/140‑1 (MQ=255)

                     aaaaaTACTGGAATACTTCGGATTCAGATACGTGGTATCAGGTGGGGTATACCAGCAGCTGGGTTGGCATCAGTTATTCGCTCTCATTTTCGTGGAATGAATCTGTAGGGAT‑‑CCCCTATAACGAACGTATTGTCGGACtt                                                                                                                   >  1:543562/1‑140 (MQ=255)

                        aaTACTGGAATACTTCGGATTCAGATACGTGGTATCAGGTGGGGTATACCAGCAGCTGGGTTGGCATCAGTTATTCGCTCTCATTTTCGTGGAATGAATCTGTAGGGAT‑‑CCCCTATAACGAACGTATTGTCGGACTTAAt                                                                                                                <  1:2626537/140‑1 (MQ=255)

                               gAATACTTCGGATTCAGATACG‑GGTATCAGGTGGGGTATACCAGCAGCTGGGTTGGCATCAGTTATTCGCTCTCATTTTCGTGGAATGAATCTGTAGGGAT‑‑CCCCTATAACGAACGTATTGTCGGACTTAATGTTTCAGt                                                                                                        <  2:2918487/140‑1 (MQ=255)

                                aaTACTTCGGATTCAGATACGTGGTATCAGGTGGGGTATACCAGCAGCTGGGTTGGCATCAGTTATTCGCTCTCATTTTCGTGGAATGAATCTGTAGGGAT‑‑CCCCTATAACGAACGTATTGTCGGACTTAATGTTTCAGt                                                                                                        <  1:1174461/140‑1 (MQ=255)

                                 aTACTTCGGATTCAGATACGTGGTATCAGGTGGGGTATACCAGCAGCTGGGTTGGCATCAGTTATTCGCTCTCATTTTCGTGGAATGAATCTGTAGGGAT‑‑CCCCTATAACGAACTTATTGTCGGACTTAATGTTTCAGTg                                                                                                       <  1:2674750/140‑1 (MQ=255)

                                  tACTTCGGATTCAGATACGTGGTATCAGGTGGGGTATACCAGCAGCTGGGTTGGCATCAGTTATTCGCTCTCATTTTCGTGGAATGAATCTGTAGGGAT‑‑CCCCTATAACGAACGTATTGTCGGACTTAATGTTTCAGTGc                                                                                                      >  1:199678/1‑140 (MQ=255)

                                   aCTTCGGATTCAGATACGTGGTATCAGGTGGGGTATACCAGCAGCTGGGTTGGCATCAGTTATTCGCTCTCATTTTCGTGGAATGAATCTGTAGGGAT‑‑CCCCTATAACGAACGTATTGTCGGACTTAATGTTTCAGTGcc                                                                                                     <  2:1146999/140‑1 (MQ=255)

                                    cTTCGGATTCAGATACGTGGTATCAGGTGGGGTATACCAGCAGCTGGGTTGGCATCAGTTATTCGCTCTCATTTTCGTGGAATGAATCTGTAGGGAT‑‑CCCCTATAACGAACGTATTGTCGGACTTAATGTTTCAGTGCCt                                                                                                    >  1:1655545/1‑140 (MQ=255)

                                     ttCGGATTCAGATACGTGGTATCAGGTGGGGTATACCAGCAGCTGGGTTGGCATCAGTTATTCGCTCTCATTTTCGTGGAATGAATCTGTAGGGAT‑‑CCCCTATAACGAACGTATTGTCGGACTTAATGTTTCAGTGCCtt                                                                                                   >  1:2530039/1‑140 (MQ=255)

                                     ttCGGATTCAGATACGTGGTATCAGGTGGGGTATACCAGCAGCTGGGTTGGCATCAGTTATTCGCTCTCATTTTCGTGGAATGAATCTGTAGGGAT‑‑CCCCTATAACGAACGTATTGTCGGACTTAATGTTTCAGTGCCtt                                                                                                   <  2:2354089/140‑1 (MQ=255)

                                     ttCGGATTCAGATACGTGGTATCAGGTGGGGTATACCAGCAGCTGGGTTGGCATCAGTTATTCGCTCTCATTTTCGTGGAATGAATCTGTAGGGAT‑‑CCCCTATAACGAACGTATTGTCGGACTTAATGTTTCAGTGCCtt                                                                                                   >  1:2572358/1‑140 (MQ=255)

                                     ttCGGATTCAGATACGTGGTATCAGGTGGGGTATACCAGCAGCTGGGTTGGCATCAGTTATTCGCTCTCATTTTCGTGGAATGAATCTGTAGGGAT‑‑CCCCTATAACGAACGTATTGTCGGACTTAATGTTTCAGTGCCtt                                                                                                   >  1:2793800/1‑140 (MQ=255)

                                       cGGATTCAGATACGTGGTATCAGGTGGGGTATACCAGCAGCTGGGTTGGCATCAGTTATTCGCTCTCATTTTCGTGGAATGAATCTGTAGGGAT‑‑CCCCTATAACGAACGTATTGTCGGACTTAATGTTTCAGTGCCTTTc                                                                                                 >  2:965642/1‑140 (MQ=255)

                                        ggATTCAGATACGTGGTATCAGGTGGGGTATACCAGCAGCTGGGTTGGCATCAGTTATTCGCTCTCATTTTCGTGGAATGAATCTGTAGGGAT‑‑CCCCTATAACGAACGTATTGTCGGACTTAATGTTTCAGTGCCTTTCa                                                                                                <  1:465531/140‑1 (MQ=255)

                                        ggATTCAGATACGTGGTATCAGGTGGGGTATACCAGCAGCTGGGTTGGCAACAGTTATTCGCTCTCATTTTCGTGGAATGAATCTGTAGGGAT‑‑CCCCTATAACGAACGTATTGTCGGACTTAATGTTTCAGTGCCTTTCa                                                                                                <  1:2102436/140‑1 (MQ=255)

                                         gATTCAGATACGTGGTATCAGGTGGGGTATACCAGCAGCTGGGTTGGCATCAGTTATTCGCTCTCATTTTCGTGGAATGAATCTGTAGGGAT‑‑CCCCTATAACGAACGTATTGTCGGACTTAATGTTTCAGTGCCTTTCaa                                                                                               >  1:1740110/1‑140 (MQ=255)

                                         gATTCAGATACGTGGTATCAGGTGGGGTATACCAGCAGCTGGGTTGGCATCAGTTATTCGCTCTCATTTTCGTGGAATGAATCTGTAGGGAT‑‑CCCCTATAACGAACGTATTGTCGGACTTAATGTTTCAGTGCCTTTCaa                                                                                               >  1:493764/1‑140 (MQ=255)

                                              aGATACGTGGTATCAGGTGGGGTATACCAGCAGCTGGGTTGGCATCAGTTATTCGCTCTCATTTTCGTGGAATGAATCTGTAGGGAT‑‑CCCCTATAACGAACGTATTGTCGGACTTAATGTTTCAGTGCCTTTCAATGttt                                                                                          >  2:946361/1‑140 (MQ=255)

                                               gATACGTGGTATCAGGTGGGGTATACCAGCAGCTGGGTTGGCATCAGTTATTCGCTCTCATTTTCGTGGAATGAATCTGTAGGGAT‑‑CCCCTATAACGAACGTATTGTCGGACTTAATGTTTCAGTGCCTTTCAATGtttt                                                                                         >  2:452635/1‑140 (MQ=255)

                                                    gTGGTATCAGGTGGGGTATACCAGCAGCTGGGTTGGCATCAGTTATTCGCTCTCATTTTCGTGGAATGAATCTGTAGGGAT‑‑CCCCTATAACGAACGTATTGTCGGACTTAATGTTTCAGTGCCTTTCAATGTTTTGACCa                                                                                    <  2:826454/140‑1 (MQ=255)

                                                      ggTATCAGGTGGGGTATACCAGCAGCTGGGTTGGCATCAGTTATTCGCTCTCATTTTCGTGGAATGAATCTGTAGGGAT‑‑CCCCTATAACGAACGTATTGTCGGACTTAATGTTTCAGTGCCTTTCAATGTTTTGACCaaa                                                                                  <  1:2420202/140‑1 (MQ=255)

                                                      ggTATCAGGTGGGGTATACCAGCAGCTGGGTTGGCATCAGTTATTCGCTCTCATTTTCGTGGAATGAATCTGTAGGGAT‑‑CCCCTATAACGAACGTATTGTCGGACTTAATGTTTCAGTGCCTTTCAATGTTTTGACCaaa                                                                                  <  2:2422147/140‑1 (MQ=255)

                                                        tATCAGGTGGGGTATACCAGCAGCTGGGTTGGCATCAGTTATTCGCTCTCATTTTCGTGGAATGAATCTGTAGGGAT‑‑CCCCTATAACGAACGTATTGTCGGACTTAATGTTTCAGTGCCTTTCAATGTTTTGACCAAACg                                                                                >  1:200387/1‑140 (MQ=255)

                                                        tATCAGGTGGGGTATACCAGCAGCTGGGTTGGCATCAGTTATTCGCTCTCATTTTCGTGGAATGAATCTGTAGGGAT‑‑CCCCTATAACGAACGTATTGTCGGACTTAATGTTTCAGTGCCTTTCAATGTTTTGACCAAACg                                                                                >  2:2732406/1‑140 (MQ=255)

                                                         aTCAGGTGGGGTATACCAGCAGCTGGGTTGGCATCAGTTATTCGCTCTCATTTTCGTGGAATGAATCTGTAGGGAT‑‑CCCCTATAACGAACGTATTGTCGGACTTAATGTTTCAGTGCCTTTCAATGTTTTGACCAAACGt                                                                               >  2:1500050/1‑140 (MQ=255)

                                                          tCAGGTGGGGTATACCAGCAGCTGGGTTGGCATCAGTTATTCGCTCTCATTTTCGTGGAATGAATCTGTAGGGAT‑‑CCCCTATAACGAACGTATTGTCGGACTTAATGTTTCAGTGCCTTTCAATGTTTTGACCAAACGTc                                                                              >  2:577447/1‑140 (MQ=255)

                                                            aGGTGGGGTATACCAGCAGCTGGGTTGGCATCAGTTATTCGCTCTCATTTTCGTGGAATGAATCTGTAGGGAT‑‑CCCCTATAACGAACGTATTGTCGGACTTAATGTTTCAGTGCCTTTCAATGTTTTGACCAAACGTCGc                                                                            <  2:2572358/140‑1 (MQ=255)

                                                             ggTGGGGTATACCAGCAGCTGGGTTGGCATCAGTTATTCGCTCTCATTTTCGTGGAATGAATCTGTAGGGAT‑‑CCCCTATAACGAACGTATTGTCGGACTTAATGTTTCAGTGCCTTTCAATGTTTTGACCAAACGTCGCt                                                                           <  2:1315079/140‑1 (MQ=255)

                                                             ggTGGGGTATACCAGCAGCTGGGTTGGCATCAGTTATTCGCTCTCATTTTCGTGGAATGAATCTGTAGGGAT‑‑CCCCTATAACGAACGTATTGTCGGACTTAATGTTTCAGTGCCTTTCAATGTTTTGACCAAACGTCGCt                                                                           <  2:2048542/140‑1 (MQ=255)

                                                             ggTGGGGTATACCAGCAGCTGGGTTGGCATCAGTTATTCGCTCTCATTTTCGTGGAATGAATCTGTAGGGAT‑‑CCCCTATAACGAACGTATTGTCGGACTTAATGTTTCAGTGCCTTTCAATGTTTTGACCAAACGTCGCt                                                                           <  1:917801/140‑1 (MQ=255)

                                                             ggTGGGGTATACCAGCAGCTGGGTTGGCATCAGTTATTCGCTCTCATTTTCGTGGAATGAATCTGTAGGGAT‑‑CCCCTATAACGAACGTATTGTCGGACTTAATGTTTCAGTGCCTTTCAATGTTTTGACCAAACGTCGCt                                                                           >  1:290278/1‑140 (MQ=255)

                                                              gTGGGGTATACCAGCAGCTGGGTTGGCATCAGTTATTCGCTCTCATTTTCGTGGAATGAATCTGTAGGGAT‑‑CCCCTATAACGAACGTATTGTCGGACTTAATGTTTCAGTGCCTTTCAATGTTTTGACCaaa                                                                                  <  2:1399437/132‑1 (MQ=255)

                                                              gTGGGGTATACCAGCAGCTGGGTTGGCATCAGTTATTCGCTCTCATTTTCGTGGAATGAATCTGTAGGGAT‑‑CCCCTATAACGAACGTATTGTCGGACTTAATGTTTCAGTGCCTTTCAATGTTTTGACCaaa                                                                                  >  1:1399437/1‑132 (MQ=255)

                                                              gTGGGGTATACCAGCAGCTGGGTTGGCATCAGTTATTCGCTCTCATTTTCGTGGAATGAATCTGTAGGGAT‑‑CCCCTATAACGAACGTATTGTCGGACTTAATGTTTCAGTGCCTTTCAATGTTTTGACCAAACGTCGCTa                                                                          >  1:956450/1‑140 (MQ=255)

                                                               tGGGGTATACCAGCAGCTGGGTTGGCATCAGTTATTCGCTCTCATTTTCGTGGAATGAATCTGTAGGGAT‑‑CCCCTATAACGAACGTATTGTCGGACTTAATGTTTCAGTGCCTTTCAATGTTTTGACCAAACGTCGCTac                                                                         <  1:1887626/140‑1 (MQ=255)

                                                                  ggTATACCAGCAGCTGGGTTGGCATCAGTTATTCGCTCTCATTTTCGTGGAATGAATCTGTAGGGAT‑‑CCCCTATAACGAACGTATTGTCGGACTTAATGTTTCAGTGCCTTTCAATGTTTTGACCAAACGTCGCTACAcc                                                                      >  2:512386/1‑140 (MQ=255)

                                                                  ggTATACCAGCAGCTGGGTTGGCATCAGTTATTCGCTCTCATTTTCGTGGAATGAATCTGTAGGGAT‑‑CCCCTATAACGAACGTATTGTCGGACTTAATGTTTCAGTGCCTTTCAATGTTTTGACCAAACGTCGCTACAcc                                                                      >  1:2221404/1‑140 (MQ=255)

                                                                      taCCAGCAGCTGGGTTGGCATCAGTTATTCGCTCTCATTTTCGTGGAATGAATCTGTAGGGAT‑‑CCCCTATAACGAACGTATTGTCGGACTTAATGTTTCAGTGCCTTTCAATGTTTTGACCAAAc                                                                                 >  2:2004906/1‑125 (MQ=255)

                                                                      taCCAGCAGCTGGGTTGGCATCAGTTATTCGCTCTCATTTTCGTGGAATGAATCTGTAGGGAT‑‑CCCCTATAACGAACGTATTGTCGGACTTAATGTTTCAGTGCCTTTCAATGTTTTGACCAAACGTCGCTACACCCggg                                                                  >  1:737433/1‑140 (MQ=255)

                                                                         cagcagCTGGGTTGGCATCAGTTATTCGCTCTCATTTTCGTGGAATGAATCTGTAGGGAT‑‑CCCCTATAACGAACGTATTGTCGGACTTAATGTTTCAGTGCCTTTCAATGTTTTGACCAAACGTCGCTACACCCGGGaaa                                                               <  2:1101327/140‑1 (MQ=255)

                                                                           gcagcTGGGTTGGCATCAGTTATTCGCTCTCATTTTCGTGGAATGAATCTGTAGGGAT‑‑CCCCTATAACGAACGTATTGTCGGACTTAATGTTTCAGTGCCTTTCAATGTTTTGACCAAACGTCGCTACACCCGGGAAAAt                                                             >  1:1315558/1‑140 (MQ=255)

                                                                            cagcTGGGTTGGCATCAGTTATTCGCTCTCATTTTCGTGGAATGAATCTGTAGGGATCCCCCCTATAACGAACGTATTGTCGGACTTAATGTTTCAGTGCCTTTCAATGTTTTGACCAAACGTCGCTACACCCGGGaaaa                                                              >  1:2487058/1‑140 (MQ=255)

                                                                            cagcTGGGTTGGCATCAGTTATTCGCTCTCATTTTCGTGGAATGAATCTGTAGGGAT‑‑CCCCTATAACGAACGTATTGTCGGACTTAATGTTTCAGTGCCTTTCAATGTTTTGACCAAACGTCGCTACACCCGGGAAAATg                                                            >  1:2472024/1‑140 (MQ=255)

                                                                            cagcTGGGTTGGCATCAGTTATTCGCTCTCATTTTCGTGGAATGAATCTGTAGGGAT‑‑CCCCTATAACGAACGTATTGTCGGACTTAATGTTTCAGTGCCTTTCAATGTTTTGACCAAACGTCGCTACACCCGGGAAAATg                                                            >  2:93682/1‑140 (MQ=255)

                                                                            cagcTGGGTTGGCATCAGTTATTCGCTCTCATTTTCGTGGAATGAATCTGTAGGGAT‑‑CCCCTATAACGAACGTATTGTCGGACTTAATGTTTCAGTGCCTTTCAATGTTTTGACCAAACGTCGCTACACCCGGGAAAATg                                                            >  2:1266206/1‑140 (MQ=255)

                                                                            cagcTGGGTTGGCATCAGTTATTCGCTCTCATTTTCGTGGAATGAATCTGTAGGGAT‑‑CCCCTATAACGAACGTATTGTCGGACTTAATGTTTCAGTGCCTTTCAATGTTTTGACCAAACGTCGCTACACCCGGGAAAATg                                                            >  2:1308876/1‑140 (MQ=255)

                                                                            cagcTGGGTTGGCATCAGTTATTCGCTCTCATTTTCGTGGAATGAATCTGTAGGGAT‑‑CCCCTATAACGAACGTATTGTCGGACTTAATGTTTCAGTGCCTTTCAATGTTTTGACCAAACGTCGCTACACCCGGGAAAATg                                                            >  2:2479473/1‑140 (MQ=255)

                                                                            cagcTGGGTTGGCATCAGTTATTCGCTCTCATTTTCGTGGAATGAATCTGTAGGGAT‑‑CCCCTATAACGAACGTATTGTCGGACTTAATGTTTCAGTGCCTTTCAATGTTTTGACCAAACGTCGCTACACCCGGGAAAATg                                                            >  1:1294768/1‑140 (MQ=255)

                                                                              gcTGGGTTGGCATCAGTTATTCGCTCTCATTTTCGTGGAATGAATCTGTAGGGAT‑‑CCCCTATAACGAACGTATTGTCGGACTTAATGTTTCAGTGCCTTTCAATGTTTTGACCAAACGTCGCTACACCCGGGAAAATgcg                                                          >  2:1848790/1‑140 (MQ=255)

                                                                              gcTGGGTTGGCATCAGTTATTCGCTCTCATTTTCGTGGAATGAATCTGTAGGGAT‑‑CCCCTATAACGAACGTATTGTCGGACTTAATGTTTCAGTGCCTTTCAATGTTTTGACCAAACGTCGCTACACCCGGGAAAATgcg                                                          >  1:2160731/1‑140 (MQ=255)

                                                                                tGGGTTGGCATCAGTTATTCGCTCTCATTTTCGTGGAATGAATCTGTAGGGAT‑‑CCCCTATAACGAACGTATTGTCGGACTTAATGTTTCAGTGCCTTTCAATGTTTTGACCAAACGTCGCTACACCCGGGAAAATGCGCt                                                        <  2:2549369/140‑1 (MQ=255)

                                                                                     tGGCATCAGTTATTCGCTCTCATTTTCGTGGAATGAATCTGTAGGGAT‑‑CCCCTATAACGAACGTATTGTCGGACTTAATGTTTCAGTGCCTTTCAATGTTTTGACCAAACGTCGCTACACCCGGGAAAATGCGCTCGAcc                                                   <  1:1041919/140‑1 (MQ=255)

                                                                                      ggCATCAGTTATTCGCTCTCATTTTCGTGGAATGAATCTGTAGGGAT‑‑CCCCTATAACGAACGTATTGTCGGACTTAATGTTTCAGTGCCTTTCAATGTTTTGACCAAACGTCGCTACACCCGGGAAAATGCGCTCGACcg                                                  >  2:2832052/1‑140 (MQ=255)

                                                                                              ttATTCGCTCTCATTTTCGTGGAATGAATCTGTAGGGAT‑‑CCCCTATAACGAACGTATTGTCGGACTTAATGTTTCAGTGCCTTTCAATGTTTTGACCAAACGTCGCTACACCCGGGAAAATGCGCTCGACCGCGCTTATg                                          <  2:1194143/140‑1 (MQ=255)

                                                                                              ttATTCGCTCTCATTTTCGTGGAATGAATCTGTAGGGAT‑‑CCCCTATAACGAACGTATTGTCGGACTTAATGTTTCAGTGCCTTTCAATGTTTTGACCAAACGTCGCTACACCCGGGAAAATGCGCTCGACCGCGCTTATg                                          <  2:1035689/140‑1 (MQ=255)

                                                                                              ttATTCGCTCTCATTTTCGTGGAATGAATCTGTAGGGAT‑‑CCCCTATAACGAACGTATTGTCGGACTTAATGTTTCAGTGCCTTTCAATGTTTTGACCAAACGTCGCTACACCCGGGAAAATGCGCTCGACCGCGCTTATg                                          >  1:20542/1‑140 (MQ=255)

                                                                                               tATTCGCTCTCATTTTCGTGGAATGAATCTGTAGGGAT‑‑CCCCTATAACGAACGTATTGTCGGACTTAATGTTTCAGTGCCTTTCAATGTTTTGACCAAACGTCGCTACACCCGGGAAAATGCGCTCGACCGCGCTTATGc                                         <  2:938783/140‑1 (MQ=255)

                                                                                                aTTCGCTCTCATTTTCGTGGAATGAATCTGTAGGGAT‑‑CCCCTATAACGAACGTATTGTCGGACTTAATGTTTCAGTGCCTTTCAATGTTTTGACCAAACGTCGCTACACCCGGGAAAATGCGCTCGACCGCGCTTATGcc                                        <  2:737433/140‑1 (MQ=255)

                                                                                                aTTCGCTCTCATTTTCGTGGAATGAATCTGTAGGGAT‑‑CCCCTATAACGAACGTATTGTCGGACTTAATGTTTCAGTGCCTTTCAATGTTTTGACCAAACGTCGCTACACCCGGGAAAATGCGCTCGACCGCGCTTATGcc                                        <  1:2343181/140‑1 (MQ=255)

                                                                                                aTTCGCTCTCATTTTCGTGGAATGAATCTGTAGGGAT‑‑CCCCTATAACGAACGTATTGTCGGACTTAATGTTTCAGTGCCTTTCAATGTTTTGACCAAACGTCGCTACACCCGGGAAAATGCGCTCGACCGCGCTTATGcc                                        <  2:199678/140‑1 (MQ=255)

                                                                                                 ttCGCTCTCATTTTCGTGGAATGAATCTGTAGGGAT‑‑CCCCTATAACGAACGTATTGTCGGACTTAATGTTTCAGTGCCTTTCAATGTTTTGACCAAACGTCGCTACACCCGGGAAAATGCGCTCGACCGCGCTTATGcct                                       <  2:1892907/140‑1 (MQ=255)

                                                                                                  tCGCTCTCATTTTCGTGGAATGAATCTGTAGGGAT‑‑CCCCTATAACGAACGTATTGTCGGACTTAATGTTTCAGTGCCTTTCAATGTTTTGACCAAACGTCGCTACACCCGGGAAAATGCGCTCGACCGCGCTTATGcctc                                      >  1:1063349/1‑140 (MQ=255)

                                                                                                      tctcATTTTCGTGGAATGAATCTGTAGGGAT‑‑CCCCTATAACGAACGTATTGTCGGACTTAATGTTTCAGTGCCTTTCAATGTTTTGACCAAACGTCGCTACACCCGGGAAAATGCGCTCGACCGCGCTTATGCCTCCttt                                  >  2:46971/1‑140 (MQ=255)

                                                                                                      tctcATTTTCGTGGAATGAATCTGTAGGGAT‑‑CCCCTATAACGAACGTATTGTCGGACTTAATGTTTCAGTGCCTTTCAATGTTTTGACCAAACGTCGCTACACCCGGGAAAATGCGCTCGACCGCGCTTATGCCTCCttt                                  >  1:715006/1‑140 (MQ=255)

                                                                                                      tctcATTTTCGTGGAATGAATCTGTAGGGAT‑‑CCCCTATAACGAACGTATTGTCGGACTTAATGTTTCAGTGCCTTTCAATGTTTTGACCAAACGTCGCTACACCCGGGAAAATGCGCTCGACCGCGCTTATGCCTCCttt                                  <  1:452635/140‑1 (MQ=255)

                                                                                                        tcATTTTCGTGGAATGAATCTGTAGGGAT‑‑CCCCTATAACGAACGTATTGTCGGACTTAATGTTTCAGTGCCTTTCAATGTTTTGACCAAACGTCGCTACACCCGGGAAAATGCGCTCGACCGCGCTTATGCCTCCTTTaa                                >  1:2933514/1‑140 (MQ=255)

                                                                                                          aTTTTCGTGGAATGAATCTGTAGGGAT‑‑CCCCTATAACGAACGTATTGTCGGACTTAATGTTTCAGTGCCTTTCAATGTTTTGACCAAACGTCGCTACACCCGGGAAAATGCGCTCGACCGCGCTTATGCCTCCTTTAACg                              <  1:1183118/140‑1 (MQ=255)

                                                                                                          aTTTTCGTGGAATGAATCTGTAGGGAT‑‑CCCCTATAACGAACGTATTGTCGGACTTAATGTTTCAGTGCCTTTCAATGTTTTGACCAAACGTCGCTACACCCGGGAAAATGCGCTCGACCGCGCTTATGCCTCCTTTAACg                              <  2:240700/140‑1 (MQ=255)

                                                                                                            tttCGTGGAATGAATCTGTAGGGAT‑‑CCCCTATAACGAACGTATTGTCGGACTTAATGTTTCAGTGCCTTTCAATGTTTTGACCAAACGTCGCTACACCCGGGAAAATGCGCTCGACCGCGCTTATGCCTCCTTTAACGcc                            <  1:832326/140‑1 (MQ=255)

                                                                                                             ttCGTGGAATGAATCTGTAGGGAT‑‑CCCCTATAACGAACGTATTGTCGGACTTAATGTTTCAGTGCCTTTCAATGTTTTGACCAAACGTCGCTACACCCGGGAAAATGCGCTCGACCGCGCTTATGCCTCCTTTAACGCCa                           <  2:461034/140‑1 (MQ=255)

                                                                                                             ttCGTGGAATGAATCTGTAGGGAT‑‑CCCCTATAACGAACGTATTGTCGGACTTAATGTTTCAGTGCCTTTCAATGTTTTGACCAAACGTCGCTACACCCGGGAAAATGCGCTCGACCGCGCTTATGCCTCCTTTAACGCCa                           <  1:2290125/140‑1 (MQ=255)

                                                                                                               cGTGGAATGAATCTGTAGGGAT‑‑CCCCTATAACGAACGTATTGTCGGACTTAATGTTTCAGTGCCTTTCAATGTTTTGACCAAACGTCGCTACACCCGGGAAAATGCGCTCGACCGCGCTTATGCCTCCTTTAACGCCAAc                         <  2:1131044/140‑1 (MQ=255)

                                                                                                               cGTGGAATGAATCTGTAGGGAT‑‑CCCCTATAACGAACGTATTGTCGGACTTAATGTTTCAGTGCCTTTCAATGTTTTGACCAAACGTCGCTACACCCGGGAAAATGCGCTCGACCGCGCTTATGCCTCCTTTAACGCCAAc                         <  1:1500050/140‑1 (MQ=255)

                                                                                                                 tGGAATGAATCTGTAGGGATCCCCCCTATAACGAACGTATTGTCGGACTTAATGTTTCAGTGCCTTTCAATGTTTTGACCAAACGTCGCTACACCCGGGAAAATGCGCTCGACCGCGCTTATGCCTCCCTTAACGCCAAc                         <  2:2487058/140‑1 (MQ=255)

                                                                                                                    aatgaatCTGTAGGGAT‑‑CCCCTATAACGAACGTATTGTCGGACTTAATGTTTCAGTGCCTTTCAATGTTTTGACCAAACGTCGCTACACCCGGGAAAATGCGCTCGACCGCGCTTATGCCTCCTTTAACGCCAACCGTaa                    <  2:543562/140‑1 (MQ=255)

                                                                                                                    aatgaatCTGTAGGGAT‑‑CCCCTATAACGAACGTATTGTCGGACTTAATGTTTCAGTGCCTTTCAATGTTTTGACCAAACGTCGCTACACCCGGGAAAATGCGCTCGACCGCGCTTATGCCTCCTTTAACGCCAACCGTaa                    >  1:1945342/1‑140 (MQ=255)

                                                                                                                         atCTGTAGGGAT‑‑CCCCTATAACGAACGTATTGTCGGACTTAATGTTTCAGTGCCTTTCAATGTTTTGACCAAACGTCGCTACACCCGGGAAAATGCGCTCGACCGCGCTTATGCCTCCTTTAACGCCAACCGTAACAGCa               <  2:800582/140‑1 (MQ=255)

                                                                                                                         atCTGTAGGGAT‑‑CCCCTATAACGAACGTATTGTCGGACTTAATGTTTCAGTGCCTTTCAATGTTTTGACCAAACGTCGCTACACCCGGGAAAATGCGCTCGACCGCGCTTATGCCTCCTTTAACGCCAACCGTAACAGCa               <  1:1236421/140‑1 (MQ=255)

                                                                                                                            tGTAGGGAT‑‑CCCCTATAACGAACGTATTGTCGGACTTAATGTTTCAGTGCCTTTCAATGTTTTGACCAAACGTCGCTACACCCGGGAAAATGCGCTCGACCGCGCTTATGCCTCCTTTAACGCCAACCGTAACAGCAACg            <  2:1740110/140‑1 (MQ=255)

                                                                                                                              tAGGGAT‑‑CCCCTATAACGAACGTATTGTCGGACTTAATGTTTCAGTGCCTTTCAATGTTTTGACCAAACGTCGCTACACCCGGGAAAATGCGCTCGACCGCGCTTATGCCTCCTTTAACGCCAACCGTAACAGCAACggg          <  2:2221404/140‑1 (MQ=255)

                                                                                                                              tAGGGAT‑‑CCCCTATAACGAACGTATTGTCGAACTTAATGTTTCAGTGCCTTTCAATGTTTTGACCAAACGTCGCTACACCCGGGAAAATGCGCTCGACCGCGCTTATGCCTCCTTTAACGCCAACCGTAACAGCAACggg          >  1:2266995/1‑140 (MQ=255)

                                                                                                                                gggAT‑‑CCCCTATAACGAACGTATTGTCGGACTTAATGTTTCAGTGCCTTTCAATGTTTTGACCAAACGTCGCTACACCCGGGAAAATGCGCTCGACCGCGCTTATGCCTCCTTTAACGCCAACCGTAACAGCAACGGGCa        >  2:1508869/1‑140 (MQ=255)

                                                                                                                                  gAT‑‑CCCCTATAACGAACGTATTGTCGGACTTAATGTTTCAGTGCCTTTCAATGTTTTGATCAAACGTCGCTACACCCGGGATAATGTGCTCGGCCGCGTTTATGCCTCCTTTAACGCCAACCGTAACAGCAACGAGCaaa      >  2:407276/1‑140 (MQ=255)

                                                                                                                                   aT‑‑CCCCTATAACGAACGTATTGTCGGACTTAATGTTTCAGTGCCTTTCAATGTTTTGACCAAACGTCGCTACACCCGGGAAAATGCGCTCGACCGCGCTTATGCCTCCTTTAACGCCAACCGTAACAGCAACGGGCaaaa     >  1:2010897/1‑140 (MQ=255)

                                                                                                                                   aT‑‑CCCCTATAACGAACGTATTGTCGGACTTAATGTTTCAGTGCCTTTCAATGTTTTGACCAAACGTCGCTACACCCGGGAAAATGCGCTCGACCGCGCTTATGCCTCCTTTAACGCCAACCGTAACAGCAACGGGCaaaa     >  1:2559086/1‑140 (MQ=255)

                                                                                                                                   aT‑‑CCCCTATAACGAACGTATTGTCGGACTTAATGTTTCAGTGCCTTTCAATGTTTTGACCAAACGTCGCTACACCCGGGAAAATGCGCTCGACCGCGCTTATGCCTCCTTTAACGCCAACCGTAACAGCAACGGGCaaaa     >  2:2618398/1‑140 (MQ=255)

                                                                                                                                      ttcccTATAATGAATGTATTGTCGGTCTTAATGTTTCAGCGCCTTTCAATGTTTTGACCAAACGTCGCTACACCCGGGAAAATGCGCTCCACCGCGCTTATGCCTCCTTTAACGCCAACCGTAACAGCACCGGGCAAAAt    >  2:1213504/3‑140 (MQ=255)

TTTATATTTCTGGTACCCATCAAAAATACTGGAATACTTCGGATTCAGATACGTGGTATCAGGTGGGGTATACCAGCAGCTGGGTTGGCATCAGTTATTCGCTCTCATTTTCGTGGAATGAATCTGTAGGGAT‑‑CCCCGATAACGAACGTATTGTCGGACTTAATGTTTCAGTGCCTTTCAATGTTTTGACCAAACGTCGCTACACCCGGGAAAATGCGCTCGACCGCGCTTATGCCTCCTTTAACGCCAACCGTAACAGCAACGGGCAAAATAG  >  NC_000913/561285‑561558

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`