Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,573,227	C→A	P93P (CCG→CCT)	eutD ←	phosphate acetyltransferase EutD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,573,227	0	C	A	100.0%	171.9 / NA	49	P93P (CCG→CCT)	eutD	phosphate acetyltransferase EutD
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (24/25);  total (24/25)

CGTAATCCGGCACGCAGCACATTTGCCGTGGAAGAGAGGTTGCCCGCGATACAGACATCCGCTTTACCGGCGCTGACCATTGCTGCGGCGAACATCAGAGGGTCGGTAAGTTTTTCCAGCGCATCCGGCGGCGTTTTTTCGCCCGCGCGGGCCAGCCAGCGATGAGCAAATTCTTCCCGCATTGCGAGGTTGCCATGCGGATCAATCACCTGTAGCCCGTCCATCGCCACGCCGTGACTGAGCGCAAACTGA  >  NC_000913/2573099‑2573350                                                                                                                                 |                                                                                                                            cGTAATCCGGCACGCAGCACATTTGCCGTGGAAGAGAGGTTGCCCGCGATACAGACATCCGCTTTACCGGCGCTGACCATTGCTGCGGCGAACATCAGAGGGTCGGTAAGTTTTTCCAGCGCATCCGGAGGCGTTTTTTc                                                                                                                  >  2:775720/1‑140 (MQ=255)   tAATCCGGCACGCAGCACATTTGCCGTGGAAGAGAGGTTGCCCGCGATACAGACATCCGCTTTACCGGTGCTGACCATTGCTGCGGCGAACATCAGAGGGTCGGTAAGTTTTTCCAGCGCATCCGGAGGCGTTTTTTCGc                                                                                                                >  1:1265146/1‑140 (MQ=255)         ggCACGCAGCACATTTGCCGTGGAAGAGAGGTTGCCCGCGATACAGACATCCGCTTTACCGGCGCTGACCATTGCTGCGGCGAACATCAGAGGGTCGGTAAGTTTTTCCAGCGCATCCGGAGGCG‑TTTTTCGCCcgcgcg                                                                                                         >  2:769449/1‑140 (MQ=255)          gCACGCAGCACATTTGCCGTGGAAGAGAGGTTGCCCGCGATACAGACATCCGCTTTACCGGCGCTGACCATTGCTGCGGCGAACATCAGAGGGTCGGTAAGTTTTTCCAGCGCATCCGGAGGCGTTTTTTCGCCcgcgcg                                                                                                         >  1:381865/1‑140 (MQ=255)            gcGCAGCACATTTGCCGTGGAAGAGAGGTTGCCCGCGATACAGACATCCGCTTTACCGGCGCTGACCATTGCTGCGGCGAACATCAGAGGGTCGGTAAGTTTTTCCAGCGCATCCGGAGGCGTTTTTTCGCCCGCGCggg                                                                                                       <  2:771083/139‑1 (MQ=255)            aCGCAGCACATTTGCCGTGGAAGAGAGGTTGCCCGCGATACAGACATCCGCTTTACCGGCGCTGACCATTGCTTCGGCGAACATCAGAGGGTCGGTAAGTTTTTCCAGCGCATCCGGAGGCGTTTTTTCGCCCGCGCggg                                                                                                       <  1:103613/140‑1 (MQ=255)                agcaCATTTGCCGTGGAAGAGAGGTTGCCCGCGATACAGACATCCGCTTTACCGGCGCTGACCATTGCTGCGGCGAACATCAGAGGGTCGGTAAGTTTTTCCAGCGCATCCGGAGGCGTTTTTTCGCCCGCGCGGgccag                                                                                                   >  2:713639/1‑140 (MQ=255)                agcaCATTTGCCGTGGAAGAGAGGTTGCCCGCGATACAGACATCCGCTTTACCGGCGCTGACCATTGCTGCGGCGAACATCAGAGGGTCGGTAAGTTTTTCCAGCGCATCCGGAGGCGTTTTTTCGCCCGCGCGGgccag                                                                                                   >  1:1267295/1‑140 (MQ=255)                       ttGCCGTGGAAGAGAGGTTGCCCGCGATACAGACATCCGCTTTACCGGCGCTGACAATTGCTGCGGCGAACATCAGAGGGTCGGTAAGTTTTTCCAGCGCATCCGGAGGCGTTTTTTCGCCCGCGCGGGCCAGCCAGCGa                                                                                            >  1:1151008/1‑140 (MQ=255)                               gAAGAGAGGTTGCCCGCGATACAGACATCCGCTTTACCGGCGCTGACCATTGCTGCGGCGAACATCAGAGGGTCGGTAAGTTTTTCCAGCGCATCCGGAGGCGTTTTTTCGCCCGCGCGGGCCAGCCAGCGATGAGCaaa                                                                                    >  2:428370/1‑140 (MQ=255)                                aaGAGAGGTTGCCCGCGATACAGACATCCGCTTTACCGGCGCTGACCATTGCTGCGGCGAACATCAGAGGGTCGGTAAGTTTTTCCAGCGCATCCGGAGGCGTTTTTTCGCCCGCGCGGGCCAGCCAGCGATGAGCAAAt                                                                                   <  1:699152/140‑1 (MQ=255)                                    gagGTTGCCCGCGATACAGACATCCGCTTTACCGGCGCTGACCATTGCTGCGGCGAACATCAGAGGGTCGGTAAGTTTTTCCAGCGCATCCGGAGGCGTTTTTTCGCCCGCGCGGGCCAGCCAGCGATGAGCAAAttctt                                                                               >  2:263161/1‑140 (MQ=255)                                            ccGCGATACAGACATCCGCTTTACCGGCGCTGACCATTGCTGCGGCGAACATCAGAGGGTCGGTAAGTTTTTCCAGCGCATCCGGAGGCGTTTTTTCGCCCGCGCGGGCCAGCCAGCGATGAGCAAATTCTTCCCGCAtt                                                                       <  1:667819/140‑1 (MQ=255)                                            ccGCGATACAGACATCCGCTTTACCGGCGCTGACCATTGCTGCGGCGAACATCAGAGGGTCGGTAAGTTTTTCCAGCGCATCCGGAGGCG‑TTTTTCGCCCGCGCGGGCCAGCCAGCGATGAGCAAATTCTTCCCGCATTg                                                                      <  1:769449/140‑1 (MQ=255)                                               cgATACAGACATCCGCTTTACCGGCGCTGACCATTGCTGCGGCGAACATCAGAGTGTCGGTAAGTTTTTCCAGCGCATCCGGAGGCGTTTTTTCGCCCGCGCGGGCCAGCCAGCGATGAGCAAATTCTTCCCGCATTGCg                                                                    <  1:1174753/140‑1 (MQ=255)                                               cgATACAGACATCCGCTTTACCGGCGCTGACCATTGCTGCGGCGAACATCAGAGGGTCGGTAAGTTTTTCCAGCGCATCCGGAGGCGTTTTTTCGCCCGCGCGGGCCAGCCAGCGATGAGCAAATTCTTCCCGCATTGCg                                                                    <  1:1447173/140‑1 (MQ=255)                                               cgATACAGACATCCGCTTTACCGGCGCTGACCATTGCTGCGGCGAACATCAGAGGGTCGGTAAGTTTTTCCAGCGCATCCGGAGGCGTTTTTTCGCCCGCGCGGGCCAGCCAGCGATGAGCAAATTCTTCCCGCATTGCg                                                                    <  1:775720/140‑1 (MQ=255)                                                        cATCCGCTTTACCGGCGCTGACCATTGCTGCGGCGAACATCAGAGGGTCGGTAAGTTTTTCCAGCGCATCCGGAGGCGTTTTTTCGCCCGCGCGGGCCAGCCAGCGATGAGCAAATTCTTCCCGCATTGCGAGGTTGCCa                                                           <  1:9290/140‑1 (MQ=255)                                                         ttCCGCTTTACCGGCGCTGACCATTGCTGTGCCGATCATCAGAGGGTCGGTAAGTTTTTCCAGCGCATCCGGAGGCGTTTTTTCGCCCGCGCGGGCCAGCCAGCGATGAGCAAATTCTTCCCGCATTGCGAGGTTGCCAt                                                          <  2:1460542/139‑1 (MQ=255)                                                         aTCCGCTTTACCGGCGCTGACCATTGCTGCGGCGAACATCAGAGGGTCGGTAAGTTTTTCCAGCGCATCCGGAGGCGTTTTTTCGCCCGCGCGGGCCAGCCAGCGATGAGCAAATTCTTCCCGCATTGCGAGGTTGcc                                                            >  2:382245/1‑138 (MQ=255)                                                         aTCCGCTTTACCGGCGCTGACCATTGCTGCGGCGAACATCAGAGGGTCGGTAAGTTTTTCCAGCGCATCCGGAGGCGTTTTTTCGCCCGCGCGGGCCAGCCAGCGATGAGCAAATTCTTCCCGCATTGCGAGGTTGcc                                                            <  1:382245/138‑1 (MQ=255)                                                           ccGCTTTACCGGCGCTGACCATTGCTGCGGCGAACATCAGAGGGTCGGTAAGTTTTCCCAGCGCATCCGGAGGCGTTTTTTCGCCCGCGCGGGCCAGCCAGCGATGAGCAAATTCTTCCCGCATTGCGAGGTTGCCATGc                                                        >  2:961791/1‑140 (MQ=255)                                                            cGCTTTACCGGCGCTGACCATTGCTGCGGCGAACATCAGAGGGTCGGTAAGTTTTTCCAGCGCATCCGGAGGCGTTTTTTCGCCCGCGCGGGCCAGCCAGCGATGAGCAAATTCTTCCCGCATTGCGAGGTTGCCATGCg                                                       >  1:1465676/1‑140 (MQ=255)                                                            cGCTTTACCGGCGCTGACCATTGCTGCGGCGAACATCAGAGGGTCGGTAAGTTTTTCCAGCGCATCCGGAGGCGTTTTTTCGCCCGCGCGGGCCAGCCAGCGATGAGCAAATTCTTCCCGCATTGCGAGGTTGCCATGCg                                                       <  1:166591/140‑1 (MQ=255)                                                            cGCTTTACCGGCGCTGACCATTGCTGCGGCGAACATCAGAGGGTCGGTAAGTTTTTCCAGCGCATCCGGAGGCGTTTTTTCGCCCGCGCGGCCCAGCCAGCGATGAGCAAATTCTTCCCGCATTGCGAGGTTGCCATGCg                                                       >  2:340203/1‑140 (MQ=255)                                                               tttACCGGCGCTGACCATTGCTGCGGCGAACATCAGAGGGTCGGTAAGTTTTTCCAGCGCATCCGGAGGCGTTTTTTCGCCCGCGCGGGCCAGCCAGCGATGAGCAAATTCTTCCCGCATTGCGAGGTTGCCATGCGGat                                                    <  2:1465676/140‑1 (MQ=255)                                                               tttACCGGCGCTGACCATTGCTGCGGCGAACATCAGAGGGTCGGTAAGTTTTTCCAGCGCATCCGGAGGCGTTTTTTCGCCCGCGCGGGCCAGCCAGCGATGAGCAAATTCTTCCCGCATTGCGAGGTTGCCATGCGGat                                                    <  1:306505/140‑1 (MQ=255)                                                                 tACCGGCGCTGACCATTGCTGCGGCGAACATCAGAGGGTCGGTAAGTTTTTCCAGCGCATCCGGAGGCGTTTTTTCGCCCGCGCGGGCCAGCCAGCGATGAGCAAATTCTTCCCGCATTGCGAGGTTGCCATGCGGatca                                                  >  2:658166/1‑140 (MQ=255)                                                                   ccGGCGCTGACCATTGCTGCGGCGAACATCAGAGGGTCGGTAAGTTTTTCCAGCGCATCCGGAGGCGTTTTTTCGCCCGCGCGGGCCAGCCAGCGATGAGCAAATTCTTCCCGCATTGCGAGGTTGCCATGCGGatcaat                                                <  1:824379/140‑1 (MQ=255)                                                                   ccGGCGCTGACCATTGCTGCGGCGAACATCAGAGGGTCGGTAAGTTTTTCCAGCGCATCCGGAGGCGTTTTTTCGCCCGCGCGGGCCAGCCAGCGATGAGCAAATTCTTCCCGCATTGCGAGGTTGCCATGCGGatcaat                                                <  1:1183694/140‑1 (MQ=255)                                                                   ccGGCGCTGACCATTGCTGCGGCGAACATCAGAGGGTCGGTAAGTTTTTCCAGCGCATCCGGAGGCGTTTTTTCGCCCGCGCGGGCCAGCCAGCGATGAGCAAATTCTTCCCGCATTGCGAGGTTGCCATGCGGatcaat                                                <  1:610332/140‑1 (MQ=255)                                                                   ccGGCGCTGACCATTGCTGCGGCGAACATCAGAGGGTCGGTAAGTTTTTCCAGCGCATCCGGAGGCGTTTTTTCGCCCGCGCGGGCCAGCCAGCGATGAGCAAATTCTTCCCGCATTGCGAGGTTGCCATGCGGatcaat                                                <  1:1259257/140‑1 (MQ=255)                                                                   ccGGCGCTGACCATTGCTGCGGCGAACATCAGAGGGTCGGTAAGTTTTTCCAGCGCATCCGGAGGCGTTTTTTCGCCCGCGCGGGCCAGCCAGCGATGAGCAAATTCTTCCCGCATTGCGAGGTTGCCATGCGGatcaat                                                <  2:381865/140‑1 (MQ=255)                                                                   ccGGCGCTGACCATTGCTGCGGCGAACATCAGAGGGTCGGTAAGTTTTTCCAGCGCATCCGGAGGCGTTTTTTCGCCCGCGCGGGCCAGCCAGCGATGAGCAAATTCTTCCCGCATTGCGAGGTTGCCATGCGGatcaat                                                <  1:1458246/140‑1 (MQ=255)                                                                      gcgcTGACCATTGCTGCGGCGAACATCAGAGGGTCGGTAAGTTTTTCCAGCGCATCCGGAGGCGTTTTTTCGCCCGCGCGGGCCAGCCAGCGATGAGCAAATTCTTCCCGCATTGCGAGGTTGCCATGCGGATCAATCAc                                             >  1:997837/1‑140 (MQ=255)                                                                                 tgctgcGGCGAACATCAGAGGGTCGGTAAGTTTTTCCAGCGCATCCGGAGGCGTTTTTTCGCCCGCGCGGGCCAGCCAGCGATGAGCAAATTCTTCCCGCATTGCGAGGTTGCCATGCGGATCAATCACCTGTAGCCCGt                                  >  2:746646/1‑140 (MQ=255)                                                                                 tgctgcGGCGAACATCAGAGGGTCGGTAAGTTTTTCCAGCGCATCCGGAGGCGTTTTTTCGCCCGCGCGGGCCAGCCAGCGATGAGCAAATTCTTCCCGCATTGCGAGGTTGCCATGCGGATCAATCACCTGTAGCCCGt                                  >  1:975422/1‑140 (MQ=255)                                                                                 tgctgcGGCGAACATCAGAGGGTCGGTAAGTTTTTACAGCGCATCCGGAGGCGTTTTTTCGCCCGCGCGGGCCAGCCAGCGATGAGCAAATTCTTCCCGCATTGCGAGGTTGCCATGCGGATCAATCACCTGTAGCCCGt                                  >  2:49734/1‑140 (MQ=255)                                                                                    tgcGGCGAACATCAGAGGGTCGGTAAGTTTTTCCAGCGCATCCGGAGGCGTTTTTTCGCCCGCGCGGGCCAGCCAGCGATGAGCAAATTCTTCCCGCATTGCGAGGTTGCCATGCGGATCAATCACCTGTAGCCCGTCCa                               <  2:1267295/140‑1 (MQ=255)                                                                                      cggcgAACATCAGAGGGTCGGTAAGTTTTTCCAGCGCATCCGGAGGCGTTTTTTCGCCCGCGCGGGCCAGCCAGCGATGAGCAAATTCTTCCCGCATTGCGAGGTTGCCATGCGGATCAATCACCTGTAGCCCGTCCCTc                             >  2:594070/1‑140 (MQ=255)                                                                                      cggcgAACATCAGAGGGTCGGTAAGTTTTTCCAGCGCATCCGGAGGCGTTTTTTCGCCCGCGCGGGCCAGCCAGCGATGAGCAAATTCTTCCCGCATTGCGAGGTTGCCATGCGGATCAATCACCTGTAGCCCGTCCATc                             >  2:1283692/1‑140 (MQ=255)                                                                                        gcgAACATCAGAGGGTCGGTAAGTTTTTCCAGCGCATCCGGAGGCGTTTTTTCGCCCGCGCGGGCCAGCCAGCGATGAGCAAATTCTTCCCGCATTGCGAGGTTGCCATGCGGATCAATCACCTGTAGCCCGTCCATCGc                           <  1:746646/140‑1 (MQ=255)                                                                                        gcgAACATCAGAGGGTCGGTAAGTTTTTCCAGCGCATCCGGAGGCGTTTTTTCGCCCGCGCGGGCCAGCCAGCGATGAGCAAATTCTTCCCGCATTGCGAGGTTGCCATGCGGATCAATCACCTGTAGCCCGTCCATCGc                           <  1:177342/140‑1 (MQ=255)                                                                                          gAACATCAGAGGGTCGGTAAGTTTTTCCAGCGCATCCGGAGGCGTTTTTTCGCCCGCGCGGGCCAGCCAGCGATGAGCAAATTCTTCCCGCATTGCGAGGTTGCCATGCGGATCAATCACCTGTAGCCCGTCCATCGCCa                         <  2:434894/140‑1 (MQ=255)                                                                                               tCAGAGGGTCGGTAAGTTTTTCCAGCGCATCCGGAGGCGTTTTTTCGCCCGCGCGGGCCAGCCAGCGATGAGCAAATTCTTCCCGCATTGCGAGGTTGCCATGCGGATCAATCACCTGTAGCCCGTCCATCGCCACGCCg                    <  2:997837/140‑1 (MQ=255)                                                                                                     ggTCGGTAAGTTTTTCCAGCGCATCCGGAGGCGTTTTTTCGCCCGCGCGGGCCAGCCAGCGATGAGCAAATTCTTCCCGCATTGCGAGGTTGCCATGCGGATCAATCACCTGTAGCCCGTCCATCGCCACGCCGTGACTg              >  2:458197/1‑140 (MQ=255)                                                                                                     ggTCGGTAAGTTTTTCCAGCGCATCCGGAGGCGTTTTTTCGCCCGCGCGGGCCAGCCAGCGATGAGCAAATTCTTCCCGCATTGCGAGGTTGCCATGCGGATCAATCACCTGTAGCCCGTCCATCGCCACGCCGTGACTg              >  1:676613/1‑140 (MQ=255)                                                                                                        cGGTAAGTTTTTCCAGCGCATCCGGAGGCGTTTTTTCGCCCGCGCGGGCCAGCCAGCGATGAGCAAATTCTTCCCGCATTGCGAGGTTGCCATGCGGATCAATCACCTGTAGCCCGTCCATCGCCACGCCGTGACTGAgc           >  1:1240153/1‑140 (MQ=255)                                                                                                         ggTAAGTTTTTCCAGCGCATCCGGAGGCGTTTTTTCGCCCGCGCGGGCCAGCCAGCGATGAGCAAATTCTTCCCGCATTGCGAGGTTGCCATGCGGATCAATCACCTGTAGCCCGTCCATCGCCACGCCGTGACTGAgcg          <  2:1093941/140‑1 (MQ=255)                                                                                                                 tttCCAGCGCATCCGGAGGCGTTTTTTCGCCCGCGCGGGCCAGCCAGCGATGAGCAAATTCTTCCCGCATTGCGAGGTTGCCATGCGGATCAATCACCTGTAGCCCGTCCATCGCCACGCCGTGACTGAGCGCAAACTGa  >  2:795992/1‑140 (MQ=255)                                                                                                                                 |                                                                                                                            CGTAATCCGGCACGCAGCACATTTGCCGTGGAAGAGAGGTTGCCCGCGATACAGACATCCGCTTTACCGGCGCTGACCATTGCTGCGGCGAACATCAGAGGGTCGGTAAGTTTTTCCAGCGCATCCGGCGGCGTTTTTTCGCCCGCGCGGGCCAGCCAGCGATGAGCAAATTCTTCCCGCATTGCGAGGTTGCCATGCGGATCAATCACCTGTAGCCCGTCCATCGCCACGCCGTGACTGAGCGCAAACTGA  >  NC_000913/2573099‑2573350

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑