Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,841,042	A→G	Q18R (CAG→CGG)	ascB →	6‑phospho‑beta‑glucosidase AscB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,841,042	0	A	G	100.0%	260.1 / NA	67	Q18R (CAG→CGG)	ascB	6‑phospho‑beta‑glucosidase AscB
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (31/36);  total (31/36)

TTGAGGAAGCGGCTGCCCAGGCGCGAAAGTATCAGAGCGTACAACCGACCGTCGCCAAAGAAGTAAGTCTTAATTGAGGATGAAAATGTCAGTATTTCCAGAAAGTTTTTTATGGGGCGGCGCGCTTGCCGCCAACCAGTCTGAAGGTGCGTTCCGTGAAGGTGACAAAGGTCTGACCACTGTCGATATGATCCCACACGGCGAGCATCGAATGGCGGTGAAACTGGGGCTGGAAAAACGTTTTCAGTTGCGAGATGACGAGTTTTATCC  >  NC_000913/2840905‑2841174                                                                                                                                          |                                                                                                                                     ttGAGGAAGCGGCTGCCCAGGCGCGAAAGTATCAGAGCGTACAACCGACCGTCGCCAAAGAAGTAAGTCTTAATTGAGGATGAAAATGTCAGTATTTCCAGAAAGTTTTTTATGGGGCGGCGCGCTTGCCGCCAACCGGt                                                                                                                                    >  1:548539/1‑140 (MQ=255)    aGGAAGCGGCTGCCCAGGCGCGAAAGTATCAGAGCGTACAACCGACCGTCGCCAAAGAAGTAAGTCTTAATTGAGGATGAAAATGTCAGTATTTCCAGAAAGTTTTTTATGGGGCGGCGCGCTTGCCGCCAACCGGTCTg                                                                                                                                 >  1:589126/1‑140 (MQ=255)      gAAGCGGCTGCCCAGGCGCGAAAGTATCAGAGCGTACAACCGACCGTCGCCAAAGAAGTAAGTCTTAATTGAGGATGAAAATGTCAGTATTTCCAGAAAGTTTTTTATGGGGCGGCGCGCTTGCCGCCAACCGGTCTGaa                                                                                                                               >  2:920006/1‑140 (MQ=255)      gAAGCGGCTGCCCAGGCGCGAAAGTATCAGAGCGTACAACCGACCGTCGCCAAAGAAGTAAGTCTTAATTGAGGATGAAAATGTCAGTATTTCCAGAAAGTTTTTTATGGGGCGGCGCGCTTGCCGCCAACCGGTCTGaa                                                                                                                               >  1:1233241/1‑140 (MQ=255)        aGCGGCTGCCCAGGCGCGAAAGTATCAGAGCGTACAACCGACCGTCGCCAAAGAAGTAAGTCTTAATTGAGGATGAAAATGTCAGTATTTCCAGAAAGTTTTTTATGGGGCGGCGCGCTTGCCGCCAACCGGTCTGAAgg                                                                                                                             >  1:1135260/1‑140 (MQ=255)        aGCGGCTGCCCAGGCGCGAAAGTATCAGAGCGTACAACCGACCGTCGCCAAAGAAGTAAGTCTTAATTGAGGATGAAAATGTCAGTATTTCCAGAAAGTTTTTTATGGGGCGGCGCGCTTGCCGCCAACCGGTCTGAAgg                                                                                                                             >  1:176172/1‑140 (MQ=255)         gCGGCTGCCCAGGCGCGAAAGTATCAGAGCGTACAACCGACCGTCGCCAAAGAAGTAAGTCTTAATTGAGGATGAAAATGTCAGTATTTCCAGAAAGTTTTTTATGGGGCGGCGCGCTTGCCGCCAACCGGTCTGAAGGt                                                                                                                            <  2:1233241/140‑1 (MQ=255)         gCGGCTGCCCAGGCGCGAAAGTATCAGAGCGTACAACCGACCGTCGCCAAAGAAGTAAGTCTTAATTGAGGATGAAAATGTCAGTATTTCCAGAAAGTTTTTTATGGGGCGGCGCGCTTGCCGCCAACCGGTCTGAAGGt                                                                                                                            <  2:878602/140‑1 (MQ=255)         gCGGCTGCCCAGGCGCGAAAGTATCAGAGCGTACAACCGACCGTCGCCAAAGAAGTAAGTCTTAATTGAGGATGAAAATGTCAGTATTTCCAGAAAGTTTTTTATGGGGCGGCGCGCTTGCCGCCAACCGGTCTGAAGGt                                                                                                                            >  2:792531/1‑140 (MQ=255)           ggCTGCCCAGGCGCGAAAGTATCAGAGCGTACAACCGACCGTCGCCAAAGAAGTAAGTCTTAATTGAGGATGAAAATGTCAGTATTTCCAGAAAGTTTTTTATGGGGCGGCGCGCTTGCCGCCAACCGGTCTGAAGGTGc                                                                                                                          <  1:920006/140‑1 (MQ=255)           ggCTGCCCAGGCGCGAAAGTATCAGAGCGTACAACCGACCGTCGCCAAAGAAGTAAGTCTTAATTGAGGATGAAAATGTCAGTATTTCCAGAAAGTTTTTTATGGGGCGGCGCGCTTGCCGCCAACCGGTCTGAAGGTGc                                                                                                                          >  2:83916/1‑140 (MQ=255)                  cAGGCGCGAAAGTATCAGAGCGTACAACCGACCGTCGCCAAAGAAGTAAGTCTTAATTGAGGATGAAAATGTCAGTATTTCCAGAAAGTTTTTTATGGGGCGGCGCGCTTGCCGCCAACCGGTCTGAAGGTGCGTTCCGt                                                                                                                   >  2:289177/1‑140 (MQ=255)                    ggCGCGAAAGTATCAGAGCGTACAACCGACCGTCGCCAAAGAAGTAAGTCTTAATTGAGGATGAAAATGTCAGTATTTCCAGAAAGTTTTTTATGGGGCGGCGCGCTTGCCGCCAACCGGTCTGAAGGTGCGTTCCGTGa                                                                                                                 <  2:633876/140‑1 (MQ=255)                     gcgcGAAAGTATCAGAGCGTACAACCGACCGTCGCCAAAGAAGTAAGTCTTAATTGAGGATGAAAATGTCAGTATTTCCAGAAAGTTTTTTATGGGGCGGCGCGCTTGCCGCCAACCGGTCTGAAGGTGCGTTCCGTGaa                                                                                                                >  1:899191/1‑140 (MQ=255)                       gcgAAAGTATCAGAGCGTACAACCGACCGTCGCCAAAGAAGTAAGTCTTAATTGAGGATGAAAATGTCAGTATTTCCAGAAAGTTTTTTATGGGGCGGCGCGCTTGCCGCCAACCGGTCTGAAGGTGCGTTCCGTGAAgg                                                                                                              <  1:480638/140‑1 (MQ=255)                          aaaGTATCAGAGCGTACAACCGACCGTCGCCAAAGAAGTAAGTCTTAATTGAGGATGAAAATGTCAGTATTTCCAGAAAGTTTTTTATGGGGCGGCGCGCTTGCCGCCAACCGGTCTGAAGGTGCGTTCCGTGAAGGTGa                                                                                                           >  1:99274/1‑140 (MQ=255)                            aGTATCAGAGCGTACAACCGACCGTCGCCAAAGAAGTAAGTCTTAATTGAGGATGAAAATGTCAGTATTTCCAGAAAGTTTTTTATGGGGCGGCGCGCTTGCCGCCAACCGGTCTGAAGGTGCGTTCCGTGAAGGTGACa                                                                                                         <  2:1153001/140‑1 (MQ=255)                            aGTATCAGAGCGTACAACCGACCGTCGCCAAAGAAGTAAGTCTTAATTGAGGATGAAAATGTCAGTATTTCCAGAAAGTTTTTTATGGGGCGGCGCGCTTGCCGCCAACCGGTCTGAAGGTGCGTTCCGTGAAGGTGACa                                                                                                         <  2:1235996/140‑1 (MQ=255)                            aGTATCAGAGCGTACAACCGACCGTCGCCAAAGAAGTAAGTCTTAATTGAGGATGAAAATGTCAGTATTTCCAGAAAGTTTTTTATGGGGCGGCGCGCTTGCCGCCAACCGGTCTGAAGGTGCGTTCCGTGAAGGTGACa                                                                                                         <  1:289177/140‑1 (MQ=255)                               aTCAGAGCGTACAACCGACCGTCGCCAAAGAAGTAAGTCTTAATTGAGGATGAAAATGTCAGTATTTCCAGAAAGTTTTTTATGGGGCGGCGCGCTTGCCGCCAACCGGTCTGAAGGTGCGTTCCGTGAAGGTGACa                                                                                                         <  2:1148813/137‑1 (MQ=255)                               aTCAGAGCGTACAACCGACCGTCGCCAAAGAAGTAAGTCTTAATTGAGGATGAAAATGTCAGTATTTCCAGAAAGTTTTTTATGGGGCGGCGCGCTTGCCGCCAACCGGTCTGAAGGTGCGTTCCGTGAAGGTGACa                                                                                                         >  1:1148813/1‑137 (MQ=255)                                 cAGAGCGTACAACCGACCGTCGCCAAAGAAGTAAGTCTTAATTGAGGATGAAAATGTCAGTATTTCCAGAAAGTTTTTTATGGGGCGGCGCGCTTGCCGCCAACCGGTCTGAAGGTGCGTTCCGTGAAGGTGACAAAGGt                                                                                                    <  1:270735/140‑1 (MQ=255)                                 cAGAGCGTACAACCGACCGTCGCCAAAGAAGTAAGTCTTAATTGAGGATGAAAATGTCAGTATTTCCAGAAAGTTTTTTATGGGGCGGCGCGCTTGCCGCCAACCGGTCTGAAGGTGCGTTCCGTGAAGGTGACAAAGGt                                                                                                    <  1:792531/140‑1 (MQ=255)                                     gCGTACAACCGACCGTCGCCAAAGAAGTAAGTCTTAATTGAGGATGAAAATGTCAGTATTTCCAGAAAGTTTTTTATGGGGCGGCGCGCTTGCCGCCAACCGGTCTGAAGGTGCGTTCCGTGAAGGTGACAAAGGTCTGa                                                                                                <  2:548539/140‑1 (MQ=255)                                      cGTACAACCGACCGTCGCCAAAGAAGTAAGTCTTAATTGAGGATGAAAATGTCAGTATTTCCAGAAAGTTTTTTATGGGGCGGCGCGCTTGCCGCCAACCGGTCTGAAGGTGCGTTCCGTGAAGGTGACAAAGGTCTGAc                                                                                               <  2:962694/140‑1 (MQ=255)                                      cGTACAACCGACCGTCGCCAAAGAAGTAAGTCTTAATTGAGGATGAAAATGTCAGTATTTCCAGAAAGTTTTTTATGGGGCGGCGCGCTTGCCGCCAACCGGTCTGAAGGTGCGTTCCGTGAAGGTGACAAAGGTCTGAc                                                                                               <  2:1135260/140‑1 (MQ=255)                                       gTACAACCGACCGTCGCCAAAGAAGTAAGTCTTAATTGAGGATGAAAATGTCAGTATTTCCAGAAAGTTTTTTATGGGGCGGCGCGCTTGCCGCCAACCGGTCTGAAGGTGCGTTCCGTGAAGGTGACAAAGGTCTGAcc                                                                                              >  2:516472/1‑140 (MQ=255)                                       gTACAACCGACCGTCGCCAAAGAAGTAAGTCTTAATTGAGGATGAAAATGTCAGTATTTCCAGAAAGTTTTTTATGGGGCGGCGCGCTTGCCGCCAACCGGTCTGAAGGTGCGTTCCGTGAAGGTGACAAAGGTCTGAcc                                                                                              >  1:1200161/1‑140 (MQ=255)                                        tACAACCGACCGTCGCCAAAGAAGTAAGTCTTAATTGAGGATGAAAATGTCAGTATTTCCAGAAAGTTTTTTATGGGGCGGCGCGCTTGCCGCCAACCGGTCTGAAGGTGCGTTCCGTGAAGGTGACAAAGGTCTGACCa                                                                                             >  1:1236410/1‑140 (MQ=255)                                        tACAACCGACCGTCGCCAAAGAAGTAAGTCTTAATTGAGGATGAAAATGTCAGTATTTCCAGAAAGTTTTTTATGGGGCGGCGCGCTTGCCGCCAACCGGTCTGAAGGTGCGTTCCGTGAAGGTGACAAAGGTCTGACCa                                                                                             >  1:1199790/1‑140 (MQ=255)                                          cAACCGACCGTCGCCAAAGAAGTAAGTCTTAATTGAGGATGAAAATGTCAGTATTTCCAGAAAGTTTTTTATGGGGCGGCGCGCTTGCCGCCAACCGGTCTGAAGGTGCGTTCCGTGACGGTGACAAAGGTCTGACCACt                                                                                           <  1:362348/140‑1 (MQ=255)                                            accgaccgTCGCCAAAGAAGTAAGTCTTAATTGAGGATGAAAATGTCAGTATTTCCAGAAAGTTTTTTATGGGGCGGCGCGCTTGCCGCCAACCGGTCTGAAGGTGCGTTCCGTGAAGGTGACAAAGGTCTGACCACTGt                                                                                         <  1:881978/140‑1 (MQ=255)                                            accgaccgTCGCCAAAGAAGTAAGTCTTAATTGAGGATGAAAATGTCAGTATTTCCAGAAAGTTTTTTATGGGGCGGCGCGCTTGCCGCCAACCGGTCTGAAGGTGCGTTCCGTGAAGGTGACAAAGGTCTGACCACTGt                                                                                         <  2:945583/140‑1 (MQ=255)                                             ccgaccgTCGCCAAAGAAGTAAGTCTTAATTGAGGATGAAAATGTCAGTATTTCCAGAAAGTTTTTTATGGGGCGGCGCGCTTGCCGCCAACCGGTCTGAAGGTGCGTTCCGTGAAGGTGACAAAGGTCTGACCACTGTc                                                                                        <  1:621939/140‑1 (MQ=255)                                             ccgaccgTCGCCAAAGAAGTAAGTCTTAATTGAGGATGAAAATGTCAGTATTTCCAGAAAGTTTTTTATGGGGCGGCGCGCTTGCCGCCAACCGGTCTGAAGGTGCGTTCCGTGAAGGTGACAAAGGTCTGACCACTGTc                                                                                        <  2:1008663/140‑1 (MQ=255)                                                accgTCGCCAAAGAAGTAAGTCTTAATTGAGGATGAAAATGTCAGTATTTCCAGAAAGTTTTTTATGGGGCGGCGCGCTTGCCGCCAACCGGTCTGAAGGTGCGTTCCGTGAAGGTGACAAAGGTCTGACCACTGTCGat                                                                                     >  2:1471430/1‑140 (MQ=255)                                                accgTCGCCAAAGAAGTAAGTCTTAATTGAGGATGAAAATGTCAGTATTTCCAGAAAGTTTTTTATGGGGCGGCGCGCTTGCCGCCAACCGGTCTGAAGGTGCGTTCCGTGAAGGTGACAAAGGTCTGACCACTGTCGa                                                                                      <  2:122629/139‑1 (MQ=255)                                                accgTCGCCAAAGAAGTAAGTCTTAATTGAGGATGAAAATGTCAGTATTTCCAGAAAGTTTTTTATGGGGCGGCGCGCTTGCCGCCAACCGGTCTGAAGGTGCGTTCCGTGAAGGTGACAAAGGTCTGACCACTGTCGa                                                                                      >  1:122629/1‑139 (MQ=255)                                                 ccgTCGCCAAAGAAGTAAGTCTTAATTGAGGATGAAAATGTCAGTATTTCCAGAAAGTTTTTTATGGGGCGGCGCGCTTGCCGCCAACCGGTCTGAAGGTGCGTTCCGTGAAGGTGACAAAGGTCTGACCACTGTCGata                                                                                    <  2:471126/140‑1 (MQ=255)                                                   gTCGCCAAAGAAGTAAGTCTTAATTGAGGATGAAAATGTCAGTATTTCCAGAAAGTTTTTTATGGGGCGGCGCGCTTGCCGCCAACCGGTCTGAAGGTGCGTTCCGTGAAGGTGACAAAGGTCTGACCACTGTCGATATg                                                                                  <  2:1387003/140‑1 (MQ=255)                                                   gTCGCCAAAGAAGTAAGTCTTAATTGAGGATGAAAATGTCAGTATTTCCAGAAAGTTTTTTATGGGGCGGCGCGCTTGCCGCCAACCGGTCTGAAGGTGCGTTCCGTGAAGGTGACAAAGGTCTGACCACTGTCGATATg                                                                                  <  2:99274/140‑1 (MQ=255)                                                     cGCCAAAGAAGTAAGTCTTAATTGAGGATGAAAATGTCAGTATTTCCAGAAAGTTTTTTATGGGGCGGCGCGCTTGCCGCCAACCGGTCTGAAGGTGCGTTCCGTGAAGGTGACAAAGGTCTGAc                                                                                               >  1:662985/1‑125 (MQ=255)                                                     cGCCAAAGAAGTAAGTCTTAATTGAGGATGAAAATGTCAGTATTTCCAGAAAGTTTTTTATGGGGCGGCGCGCTTGCCGCCAACCGGTCTGAAGGTGCGTTCCGTGAAGGTGACAAAGGTCTGAc                                                                                               <  2:662985/125‑1 (MQ=255)                                                     cGCCAAAGAAGTAAGTCTTAATTGAGGATGAAAATGTCAGTATTTCCAGAAAGTTTTTTATGGGGCGGCGCGCTTGCCGCCAACCGGTCTGAAGGTGCGTTCCGTGAAGGTGACAAAGGTCTGACCACTGTCGATATGAt                                                                                >  1:236860/1‑140 (MQ=255)                                                       ccAAAGAAGTAAGTCTTAATTGAGGATGAAAATGTCAGTATTTCCAGAAAGTTTTTTATGGGGCGGCGCGCTTGCCGCCAACCGGTCTGAAGGTGCGTTCCGTGAAGGTGACAAAGGTCTGACCACTGTCGATATGATcc                                                                              >  2:821395/1‑140 (MQ=255)                                                          aagaagTAAGTCTTAATTGAGGATGAAAATGTCAGTATTTCCAGAAAGTTTTTTATGGGGCGGCGCGCTTGCCGCCAACCGGTCTGAAGGTGCGTTCCGTGAAGGTGACAAAGGTCTGACCACTGTCGATATGATCCcac                                                                           <  1:194471/140‑1 (MQ=255)                                                               gtaagtCTTAATTGAGGATGAAAATGTCAGTATTTCCAGAAAGTTTTTTATGGGGCGGCGCGCTTGCCGCCAACCGGTCTGAAGGTGCGTTCCGTGAAGGTGACAAAGGTCTGACCACTGTCGATATGATCCCACACGGc                                                                      <  2:935705/140‑1 (MQ=255)                                                               gtaagtCTTAATTGAGGATGAAAATGTCAGTATTTCCAGAAAGTTTTTTATGGGGCGGCGCGCTTGCCGCCAACCGGTCTGAAGGTGCGTTCCGTGAAGGTGACAAAGGTCTGACCACTGTCGATATGATCCCACACGGc                                                                      >  2:1274647/1‑140 (MQ=255)                                                                              ggATGAAAATGTCAGTATTTCCAGAAAGTTTTTTATGGGGCGGCGCGCTTGCCGCCAACCGGTCTGAAGGTGCGTTCCGTGAAGGTGACAAAGGTCTGACCACTGTCGATATGATCCCACACGGCGAGCATCGAATGGCg                                                       >  2:1124896/1‑140 (MQ=255)                                                                              ggATGAAAATGTCAGTATTTCCAGAAAGTTTTTTATGGGGCGGCGCGCTTGCCGCCAACCGGTCTGAAGGTGCGTTCCGTGAAGGTGACAAAGGTCTGACCACTGTCGATATGATCCCACACGGCGAGCATCGAATGGCg                                                       >  2:926187/1‑140 (MQ=255)                                                                               gATGAAAATGTCAGTATTTCCAGAAAGTTTTTTATGGGGCGGCGCGCTTGCCGCCAACCGGTCTGAAGGTGCGTTCCGTGAAGGTGACAAAGGTCTGACCACTGTCGATATGATCCCACACGGCGAGCATCGAATGGCgg                                                      <  2:1161768/140‑1 (MQ=255)                                                                               gATGAAAATGTCAGTATTTCCAGAAAGTTTTTTATGGGGCGGCGCGCTTGCCGCCAACCGGTCTGAAGGTGCGTTCCGTGAAGGTGACAAAGGTCTGACCACTGTCGATATGATCCCACACGGCGAGCATCGAATGGCgg                                                      >  1:1467749/1‑140 (MQ=255)                                                                                aTGAAAATGTCAGTATTTCCAGAAAGTTTTTTATGGGGCGGCGCGCTTGCCGCCAACCGGTCTGAAGGTGCGTTCCGTGAAGGTGACAAAGGTCTGACCACTGTCGATATGATCCCACACGGCGAGCATCGAAt                                                           <  2:212538/134‑1 (MQ=255)                                                                                aTGAAAATGTCAGTATTTCCAGAAAGTTTTTTATGGGGCGGCGCGCTTGCCGCCAACCGGTCTGAAGGTGCGTTCCGTGAAGGTGACAAAGGTCTGACCACTGTCGATATGATCCCACACGGCGAGCATCGAAt                                                           >  1:212538/1‑134 (MQ=255)                                                                                 tGAAAATGTCAGTATTTCCAGAAAGTTTTTTATGGGGCGGCGCGCTTGCCGCCAACCGGTCTGAAGGTGCGTTCCGTGAAGGTGACAAAGGTCTGACCACTGTCGATATGATCCCACACGGCGAGCATCGAATGGCGGTg                                                    >  1:518199/1‑140 (MQ=255)                                                                                  gAAAATGTCAGTATTTCCAGAAAGTTTTTTATGGGGCGGCGCGCTTGCCGCCAACCGGTCTGAAGGTGCGTTCCGTGAAGGTGACAAAGGTCTGACCACTGTCGATATGATCCCACACGGCGAGCATCGAATGGCGGTGa                                                   >  1:1181844/1‑140 (MQ=255)                                                                                     aaTGTCAGTATTTCCAGAAAGTTTTTTATGGGGCGGCGCGCTTGCCGCCAACCGGTCTGAAGGTGCGTTCCGTGAAGGTGACAAAGGTCTGACCACTGTCGATATGATCCCACACGGCGAGCATCGAATGGCGGTGAAAc                                                <  1:821395/140‑1 (MQ=255)                                                                                      aTGTCAGTATTTCCAGAAAGTTTTTTATGGGGCGGCGCGCTTGCCGCCAACCGGTCTGAAGGTGCGTTCCGTGAAGGTGACAAAGGTCTGACCACTGTCGATATGATCCCACACGGCGAGCATCGAATGGCGGTGAAACt                                               <  2:176172/140‑1 (MQ=255)                                                                                        gTCAGTATTTCCAGAAAGTTTTTTATGGGGCGGCGCGCTTGCCGCCAACCGGTCTGAAGGTGCGTTCCGTGAAGGTGACAAAGGTCTGACCACTGTCGATATGATCCCACACGGCGAGCATCGAATGGCGGTGAAACTgg                                             >  1:303693/1‑140 (MQ=255)                                                                                               tttCCAGAAAGTTTTTTATGGGGCGGCGCGCTTGCCGCCAACCGGTCTGAAGGTGCGTTCCGTGAAGGTGACAAAGGTCTGACCACTGTCGATATGATCCCACACGGCGAGCATCGAATGGCGGTGAAACTGGGGc                                          <  1:1181498/136‑1 (MQ=255)                                                                                               tttCCAGAAAGTTTTTTATGGGGCGGCGCGCTTGCCGCCAACCGGTCTGAAGGTGCGTTCCGTGAAGGTGACAAAGGTCTGACCACTGTCGATATGATCCCACACGGCGAGCATCGAATGGCGGTGAAACTGGGGc                                          >  2:1181498/1‑136 (MQ=255)                                                                                                       aaGTTTTTTATGGGGCGGCTCGCTTGCCGCCAACCGGTCTGAAGGGGCGTTCCGTGAAGGCGACAAAGTTCTGACCACTGTCGATATGATCCCACACGGCGAGCATCGAATGGCGGGGAAACTTGGGCCGGAAAAACgct                              >  2:348346/1‑138 (MQ=255)                                                                                                       aaGTTTTTTATGGGGCGGCGCGCTTGCCGCCAACCGGTCTGAAGGTGCGTTCCGTGAAGGTGACAAAGGTCTGACCACTGTCGATATGATCCCACACGGCGAGCATCGAATGGCGGTGAAACTGGGGCTGGAAAAACGtt                              <  2:1181844/140‑1 (MQ=255)                                                                                                            ttttATGGGGCGGCGCGCTTGCCGCCAACCGGTCTGAAGGTGCGTTCCGTGAAGGTGACAAAGGTCTGACCACTGTCGATATGATCCCACACGGCGAGCATCGAATGGCGGTGAAACTGGGGCTGGAAAAACGTTTTCAg                         <  2:236860/140‑1 (MQ=255)                                                                                                                        gcgcgcTTGCCGCCAACCGGTCTGAAGGTGCGTTCCGTGAAGGTGACAAAGGTCTGACCACTGTCGATATGATCCCACACGGCGAGCATCGAATGGCGGTGAAACTGGGGCTGGAAAAACGTTTTCAGTTGCGAGATGAc             <  2:888961/140‑1 (MQ=255)                                                                                                                             cTTGCCGCCAACCGGTCTGAAGGTGCGTTCCGTGAAGGTGACAAAGGTCTGACCACTGTCGATATGATCCCACACGGCGAGCATCGAATGGCGGTGAAACTGGGGCTGGAAAAACGTTTTCAGTTGCgag                  <  2:614607/130‑1 (MQ=255)                                                                                                                             cTTGCCGCCAACCGGTCTGAAGGTGCGTTCCGTGAAGGTGACAAAGGTCTGACCACTGTCGATATGATCCCACACGGCGAGCATCGAATGGCGGTGAAACTGGGGCTGGAAAAACGTTTTCAGTTGCgag                  >  1:614607/1‑130 (MQ=255)                                                                                                                               tGCCGCCAACCGGTCTGAAGGTGCGTTCCGTGAAGGTGACAAAGGTCTGACCACTGTCGATATGATCCCACACGGCGAGCATCGAATGGCGGTGAAACTGGGGCTGGAAAAACGTTTTCAGTTGCGAGATGACGAGtttt      >  1:1118485/1‑140 (MQ=255)                                                                                                                                   gccAACCGGTCTGAAGGTGCGTTCCGTGAAGGTGACAAAGGTCTGACCACTGTCGATATGATCCCACACGGCGAGCATCGAATGGCGGTGAAACTGGGGCTGGAAAAACGTTTTCAGTTGCGAGATGACGAGTTTTATcc  <  1:516472/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                     TTGAGGAAGCGGCTGCCCAGGCGCGAAAGTATCAGAGCGTACAACCGACCGTCGCCAAAGAAGTAAGTCTTAATTGAGGATGAAAATGTCAGTATTTCCAGAAAGTTTTTTATGGGGCGGCGCGCTTGCCGCCAACCAGTCTGAAGGTGCGTTCCGTGAAGGTGACAAAGGTCTGACCACTGTCGATATGATCCCACACGGCGAGCATCGAATGGCGGTGAAACTGGGGCTGGAAAAACGTTTTCAGTTGCGAGATGACGAGTTTTATCC  >  NC_000913/2840905‑2841174

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑