Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,057,387	G→T	H342N (CAC→AAC)	serA ←	phosphoglycerate dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,057,387	0	G	T	100.0%	257.8 / NA	74	H342N (CAC→AAC)	serA	phosphoglycerate dehydrogenase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (31/43);  total (31/43)

TCGTCGGCTTCAATATCAATAACCACATAACCCATCTGGGCGGAAGTTTGCAGATATTGCGCGGCGATGTTGACGCCCTGCTCGGCGAAGATTTTGTTCAGCGCAGTTAGCACGCCCGGACGGTTTTCGTGGATGTGCATCAGACGACGCCCACCGTGCAGTGGCAGCGAGACTTCCGGGAAGTTCACCGCAGAGAGCGTTGAGCCATTGTCAGAATACTTGATCAATTTACCCGCAACTTCCAGGCCGATATTCTCCTGCGCTTCC  >  NC_000913/3057251‑3057517                                                                                                                                         |                                                                                                                                   tcgtcgGCTTCAATATCAATAACCACATAACCCATCTGGGCGGAAGTTTGCAGATATTGCGCGGCGATGTTGACGCCCTGCTCGGCGAAGATTTTGTTCAGCGCAGTTAGCACGCCCGGACGGTTTTCGTGGATGTTCAt                                                                                                                                 <  2:1455148/140‑1 (MQ=255)  cgtcgGCTTCAATATCAATAACCACATAACCCATCTGGGCGGAAGTTTGCAGATATTGCGCGGCGATGTTGACGCCCTGCTCGGCGAAGATTTTGTTCAGCGCAGTTAGCACGCCCGGACGGTTTTCGTGGATGTTCATc                                                                                                                                <  1:239196/140‑1 (MQ=255)        cTTCAATATCAATAACCACATAACCCATCTGGGCGGAAGTTTGCAGATATTGCGCGGCGATGTTGACGCCCTGCTCGGCGAAGATTTTGTTCAGCGCAGTTAGCACGCCCGGACGGTTTTCGTGGATGTTCATCAgacga                                                                                                                          >  2:113616/1‑140 (MQ=255)           caatatcaataACCACATAACCCATCTGGGCGGAAGTTTGCAGATATTGCGCGGCGATGTTGACGCCCTGCTCGGCGAAGATTTTGTTCAGCGCAGTTAGCACGCCCGGACGGTTTTCGTGGATGTTCATCAGACGACGc                                                                                                                       <  1:652593/140‑1 (MQ=255)           caatatcaataACCACATAACCCATCTGGGCGGAAGTTTGCAGATATTGCGCGGCGATGTTGACGCCCTGCTCGGCGAAGATTTTGTTCAGCGCAGTTAGCACGCCCGGACGGTTTTCGTGGATGTTCATCAGACGACGc                                                                                                                       <  2:1419445/140‑1 (MQ=255)               atcaataACCACATAACCCATCTGGGCGGAAGTTTGCAGATATTGCGCGGCGATGTTGACGCCCTGCTCGGCGAAGATTTTGTTCAGCGCAGTTAGCACGCCCGGACGGTTTTCGTGGATGTTCATCAGACGACGCCCAc                                                                                                                   <  2:1013353/140‑1 (MQ=255)                   ataACCACATAACCCATCTGGGCGGAAGTTTGCAGATATTGCGCGGCGATGTTGACGCCCTGCTCGGCGAAGATTTTGTTCAGCGCAGTTAGCACGCCCGGACGGTTTTCGTGGATGTTCATCAGACGACGCCCACCGTg                                                                                                               >  2:904484/1‑140 (MQ=255)                    taACCACATAACCCATCTGGGCGGAAGTTTGCAGATATTGCGCGGCGATGTTGACGCCCTGCTCGGCGAAGATTTTGTTCAGCGCAGTTAGCACGCCCGGACGGTTTTCGTGGATGTTCATCAGACGACGCCCACCGTGc                                                                                                              <  2:1183488/140‑1 (MQ=255)                      aCCACATAACCCATCTGGGCGGAAGTTTGCAGATATTGCGCGGCGATGTTGACGCCCTGCTCGGCGAAGATTTTGTTCAGCGCAGTTAGCACGCCCGGACGGTTTTCGTGGATGTTCATCAGACGACGCCCACCg                                                                                                                 >  1:1165025/1‑135 (MQ=255)                      aCCACATAACCCATCTGGGCGGAAGTTTGCAGATATTGCGCGGCGATGTTGACGCCCTGCTCGGCGAAGATTTTGTTCAGCGCAGTTAGCACGCCCGGACGGTTTTCGTGGATGTTCATCAGACGACGCCCACCg                                                                                                                 <  2:1165025/135‑1 (MQ=255)                      aCCACATAACCCATCTGGGCGGAAGTTTGCAGATATTGCGCGGCGATGTTGACGCCCTGCTCGGCGAAGATTTTGTTCAGCGCAGTTAGCACGCCCGGACGGTTTTCGTGGATGTTCATCAGACGACGCCCACCGTGCAg                                                                                                            >  1:1186323/1‑140 (MQ=255)                          caTAACCCATCTGGGCGGAAGTTTGCAGATATTGCGCGGCGATGTTGACGCCCTGCTCGGCGAAGATTTTGTTCAGCGCAGTTAGCACGCCCGGACGGTTTTCGTGGATGTTCATCAGACGACGCCCACCGTGCAGTGGc                                                                                                        >  1:551350/1‑140 (MQ=255)                            tAACCCATCTGGGCGGAAGTTTGCAGATATTGCGCGGCGATGTTGACGCCCTGCTCGGCGAAGATTTTGTTCAGCGCAGTTAGCACGCCCGGACGGTTTTCGTGGATGTTCATCAGACGACGCCCACCGTGCAGTGGCAg                                                                                                      >  1:11801/1‑140 (MQ=255)                            tAACCCATCTGGGCGGAAGTTTGCAGATATTGCGCGGCGATGTTGACGCCCTGCTCGGCGAAGATTTTGTTCAGCGCAGTTAGCACGCCCGGACGGTTTTCGTGGATGTTCATCAGACGACGCCCACCGTGCAGTGGCAg                                                                                                      >  1:854206/1‑140 (MQ=255)                            tAACCCATCTGGGCGGAAGTTTGCAGATATTGCGCGGCGATGTTGACGCCCTGCTCGGCGAAGATTTTGTTCAGCGCAGTTAGCACGCCCGGACGGTTTTCGTGGATGTTCATCAGACGACGCCCACCGTGCAGTGGCAg                                                                                                      >  1:68348/1‑140 (MQ=255)                               cccATCTGGGCGGAAGTTTGCAGATATTGCGCGGCGATGTTGACGCCCTGCTCGGCGAAGATTTTGTTCAGCGCAGTTAGCACGCCCGGACGGTTTTCGTGGATGTTCATCAGACGACGCCCACCGTGCAGTGGCAGCga                                                                                                   >  2:857349/1‑140 (MQ=255)                               cccATCTGGGCGGAAGTTTGCAGATATTGCGCGGCGATGTTGACGCCCTGCTCGGCGAAGATTTTGTTCAGCGCAGTTAGCACGCCCGGACGGTTTTCGTGGATGTTCATCAGACGACGCCCACCGTGCAGTGGCAGCga                                                                                                   >  1:762053/1‑140 (MQ=255)                                  aTCTGGGCGGAAGTTTGCAGATATTGCGCGGCGATGTTGACGCCCTGCTCGGCGAAGATTTTGTTCAGCGCAGTTAGCACGCCCGGACGGTTTTCGTGGATGTTCATCAGACGACGCCCACCGTGCAGTGGCAGCGAGAc                                                                                                >  2:407141/1‑140 (MQ=255)                                  aTCTGGGCGGAAGTTTGCAGATATTGCGCGGCGATGTTGACGCCCTGCTCGGCGAAGATTTTGTTCAGCGCAGTTAGCACGCCCGGACGGTTTTCGTGGATGTTCATCAGACGACGCCCACCGTGCAGTGGCAGCGAGAc                                                                                                >  2:1141062/1‑140 (MQ=255)                                  aTCTGGGCGGAAGTTTGCAGATATTGCGCGGCGATGTTGACGCCCTGCTCGGCGAAGATTTTGTTCAGCGCAGTTAGCACGCCCGGACGGTTTTCGTGGATGTTCATCAGACGACGCCCACCGTGCAGTGGCAGCGAGAc                                                                                                >  1:591131/1‑140 (MQ=255)                                    cTGGGCGGAAGTTTGCAGATATTGCGCGGCGATGTTGACGCCCTGCTCGGCGAAGATTTTGTTCAGCGCAGTTAGCACGCCCGGACGGTTTTCGTGGATGTTCATCAGACGACGCCCACCGTGCAGTGGCAGCGAGACtt                                                                                              <  2:1186323/140‑1 (MQ=255)                                    cTGGGCGGAAGTTTGCAGATATTGCGCGGCGATGTTGACGCCCTGCTCGGCGAAGATTTTGTTCAGCGCAGTTAGCACGCCCGGACGGTTTTCGTGGATGTTCATCAGACGACGCCCACCGTGCAGTGGCAGCGAGACtt                                                                                              <  2:518051/140‑1 (MQ=255)                                       ggCGGAAGTTTGCAGATATTGCGCGGCGATGTTGACGCCCTGCTCGGCGAAGATTTTGTTCAGCGCAGTTAGCACGCCCGGACGGTTTTCGTGGATGTTCATCAGACGACGCCCACCGTGCAGTGGCAGCGAGACTTCCg                                                                                           >  1:285729/1‑140 (MQ=255)                                         cGGAAGTTTGCAGATATTGCGCGGCGATGTTGACGCCCTGCTCGGCGAAGATTTTGTTCAGCGCAGTTAGCACGCCCGGACGGTTTTCGTGGATGTTCATCAGACGACGCCCACCGTGCAGTGGCAGCGAGACTTCCggg                                                                                         >  2:362551/1‑140 (MQ=255)                                         cGGAAGTTTGCAGATATTGCGCGGCGATGTTGACGCCCTGCTCGGCGAAGATTTTGTTCAGCGCAGTTAGCACGCCCGGACGGTTTTCGTGGATGTTCATCAGACGACGCCCACCGTGCAGTGGCAGCGAGACTTCCggg                                                                                         <  2:569048/140‑1 (MQ=255)                                         cGGAAGTTTGCAGATATTGCGCGGCGATGTTGACGCCCTGCTCGGCGAAGATTTTGTTCAGCGCAGTTAGCACGCCCGGACGGTTTTCGTGGATGTTCATCAGACGACGCCCACCGTGCAGTGGCAGCGAGACTTCCggg                                                                                         <  2:860057/140‑1 (MQ=255)                                         cGGAAGTTTGCAGATATTGCGCGGCGATGTTGACGCCCTGCTCGGCGAAGATTTTGTTCAGCGCAGTTAGCACGCCCGGACGGTTTTCGTGGATGTTCATCAGACGACGCCCACCGTGCAGTGGCAGCGAGACTTCCggg                                                                                         <  1:113616/140‑1 (MQ=255)                                         cGGAAGTTTGCAGATATTGCGCGGCGATGTTGACGCCCTGCTCGGCGAAGATTTTGTTCAGCGCAGTTAGCACGCCCGGACGGTTTTCGTGGATGTTCATCAGACGACGCCCACCGTGCAGTGGCAGCGAGACTTCCggg                                                                                         >  1:533225/1‑140 (MQ=255)                                         cGGAAGTTTGCAGATATTGCGCGGCGATGTTGACGCCCTGCTCGGCGAAGATTTTGTTCAGCGCAGTTAGCACGCCCGGACGGTTTTCGTGGATGTTCATCAGACGACGCCCACCGTGCAGTGGCAGCGAGACTTCCggg                                                                                         <  1:190918/140‑1 (MQ=255)                                           gAAGTTTGCAGATATTGCGCGGCGATGTTGACGCCCTGCTCGGCGAAGATTTTGTTCAGCGCAGTTAGCACGCCCGGACGGTTTTCGTGGATGTTCATCAGACGACGCCCACCGTGCAGTGGCAGCGAGACTTCCGGGaa                                                                                       <  2:496327/140‑1 (MQ=255)                                             aGTTTGCAGATATTGCGCGGCGATGTTGACGCCCTGCTCGGCGAAGATTTTGTTCAGCGCAGTTAGCACGCCCGGACGGTTTTCGTGGATGTTCATCAGACGACGCCCACCGTGCAGTGGCAGCGAGACTTCCGGGAAGt                                                                                     <  2:841165/140‑1 (MQ=255)                                             aGTTTGCAGATATTGCGCGGCGATGTTGACGCCCTGCTCGGCGAAGATTTTGTTCAGCGCAGTTAGCACGCCCGGACGGTTTTCGTGGATGTTCATCAGACGACGCCCACCGTGCAGTGGCAGCGAGACTTCCGGGAAGt                                                                                     <  2:1129498/140‑1 (MQ=255)                                               tttGCAGATATTGCGCGGCGATGTTGACGCCCTGCTCGGCGAAGATTTTGTTCAGCGCAGTTAGCACGCCCGGACGGTTTTCGTGGATGTTCATCA‑‑‑GACGCCCACCGTGCAGTGGCAGCGAGACTTCCGGGAAGTTCAcc                                                                                <  2:930570/140‑1 (MQ=255)                                                ttGCAGATATTGCGCGGCGATGTTGACGCCCTGCTCGGCGAAGATTTTGTTCAGCGCAGTTAGCACGCCCGGACGGTTTTCGTGGATGTTCATCAGACGACGCCCACCGTGCAGTGGCAGCGAGACTTCCGGGAAGTTCa                                                                                  <  2:854206/140‑1 (MQ=255)                                                      atatTGCGCGGCGATGTTGACGCCCTGCTCGGCGAAGATTTTGTTCAGCGCAGTTAGCACGCCCGGACGGTTTTCGTGGATGTTCATCAGACGACGCCCACCGTGCAGTGGCAGCGAGACTTCCGGGAAGTTCACCGCag                                                                            >  1:122331/1‑140 (MQ=255)                                                        atTGCGCGGCGATGTTGGCGCCCTGCTCGGCGAAGATTTTGTTCAGCGCAGTTAGCACGCCCGGACGGTTTTCGTGGATGTTCATCAGACGACGCCCACCGTGCAGTGGCAGCGAGACTTCCGGGAAGTTCACCGCagag                                                                          <  2:1034206/140‑1 (MQ=255)                                                           gcgcGGCGATGTTGACGCCCTGCTCGGCGAAGATTTTGTTCAGCGCAGTTAGCACGCCCGGACGGTTTTCGTGGATGTTCATCAGACGACGCCCACCGTGCAGTGGCAGCGAGACTTCCGGGaa                                                                                       >  1:367101/1‑124 (MQ=255)                                                           gcgcGGCGATGTTGACGCCCTGCTCGGCGAAGATTTTGTTCAGCGCAGTTAGCACGCCCGGACGGTTTTCGTGGATGTTCATCAGACGACGCCCACCGTGCAGTGGCAGCGAGACTTCCGGGaa                                                                                       <  2:367101/124‑1 (MQ=255)                                                              cggcgATGTTGACGCCCTGCTCGGCGAAGATTTTGTTCAGCGCAGTTAGCACGCCCGGACGGTTTTCGTGGATGTTCATCAGACGACGCCCACCGTGCAGTGGCAGCGAGACTTCCGGGAAGTTCACCGCAGAGAGCGtt                                                                    <  2:621962/140‑1 (MQ=255)                                                              cggcgATGTTGACGCCCTGCTCGGCGAAGATTTTGTTCAGCGCAGTTAGCACGCCCGGACGGTTTTCGTGGATGTTCATCAGACGACGCCCACCGTGCAGTGGCAGCGAGACTTCCGGGAAGTTCACCGCAGAGAGCGtt                                                                    >  1:1269599/1‑140 (MQ=255)                                                                 cgATGTTGACGCCCTGCTCGGCGAAGATTTTGTTCAGCGCAGTTAGCACGCCCGGACGGTTTTCGTGGATGTTCATCAGACGACGCCCACCGTGCAGTGGCAGCGAGACTTCCGGGAAGTTCACCGCAGAGAGCGTTGAg                                                                 >  2:1244340/1‑140 (MQ=255)                                                                 cgATGTTGACGCCCTGCTCGGCGAAGATTTTGTTCAGCGCAGTTAGCACGCCCGGACGGTTTTCGTGGATGTTCATCAGACGACGCCCACCGTGCAGTGGCAGCGAGACTTCCGGGAAGTTCACCGCAGAGAGCGTTGAg                                                                 <  2:1411384/140‑1 (MQ=255)                                                                 cgATGTTGACGCCCTGCTCGGCGAAGATTTTGTTCAGCGCAGTTAGCACGCCCGGACGGTTTTCGTGGATGTTCATCAGACGACGCCCACCGTGCAGTGGCAGCGAGACTTCCGGGAAGTTCACCGCAGAGAGCGTTGAg                                                                 >  2:1188128/1‑140 (MQ=255)                                                                 cgATGTTGACGCCCTGCTCGGCGAAGATTTTGTTCAGCGCAGTTAGCACGCCCGGACGGTTTTCGTGGATGTTCATCAGACGACGCCCACCGTGCAGTGGCAGCGAGACTTCCGGGAAGTTCACCGCAGAGAGCGTTGAg                                                                 >  1:1411384/1‑140 (MQ=255)                                                                 cgATGTTGACGCCCTGCTCGGCGAAGATTTTGTTCAGCGCAGTTAGCACGCCCGGACGGTTTTCGTGGATGTTCATCAGACGACGCCCACCGTGCAGTGGCAGCGAGACTTCCGGGAAGTTCACCGCAGAGAGCGTTGAg                                                                 >  2:108648/1‑140 (MQ=255)                                                                   aTGTTGACGCCCTGCTCGGCGAAGATTTTGTTCAGCGCAGTTAGCACGCCCGGACGGTTTTCGTGGATGTTCATCAGACGACGCCCACCGTGCAGTGGCAGCGAGACTTCCGGGAAGTTCACCGCAGAGAGCGTTGAGcc                                                               <  1:227145/140‑1 (MQ=255)                                                                   aTGTTGACGCCCTGCTCGGCGAAGATTTTGTTCAGCGCAGTTAGCACGCCCGGACGGTTTTCGTGGATGTTCATCAGACGACGCCCACCGTGCAGTGGCAGCGAGACTTCCGGGAAGTTCACCGCAGAGAGCGTTGAGcc                                                               <  1:812873/140‑1 (MQ=255)                                                                   aTGTTGACGCCCTGCTCGGCGAAGATTTTGTTCAGCGCAGTTAGCACGCCCGGACGGTTTTCGTGGATGTTCATCAGACGACGCCCACCGTGCAGTGGCAGCGAGACTTCCGGGAAGTTCACCGCAGAGAGCGTTGAGcc                                                               <  1:312082/140‑1 (MQ=255)                                                                   aTGTTGACGCCCTGCTCGGCGAAGATTTTGTTCAGCGCAGTTAGCACGCCCGGACGGTTTTCGTGGATGTTCATCAGACGACGCCCACCGTGCAGTGGCAGCGAGACTTCCGGGAAGTTCACCGCAGAGAGCGTTGAGcc                                                               <  1:1188128/140‑1 (MQ=255)                                                                     gTTGACGCCCTGCTCGGCGAAGATTTTGTTCAGCGCAGTTAGCACGCCCGGACGGTTTTCGTGGATGTTCATCAGACGACGCCCACCGTGCAGTGGCAGCGAGACTTCCGGGAAGTTCACCGCAGAGAGCGTTGAGCCAt                                                             <  2:571628/140‑1 (MQ=255)                                                                     gTTGACGCCCTGCTCGGCGAAGATTTTGTTCAGCGCAGTTAGCACGCCCGGACGGTTTTCGTGGATGTTCATCAGACGACGCCCACCGTGCAGTGGCAGCGAGACTTCCGGGAAGTTCACCGCAGAGAGCGTTGAGCCAt                                                             <  1:362551/140‑1 (MQ=255)                                                                          cGCCCTGCTCGGCGAAGATTTTGTTCAGCGCAGTTAGCACGCCCGGACGGTTTTCGTGGATGTTCATCAGACGACGCCCACCGTGCAGTGGCAGCGAGACTTCCGGGAAGTTCACCGCAGAGAGCGTTGAGCCATTGTCa                                                        <  2:68348/140‑1 (MQ=255)                                                                                gCTCGGCGAAGATTTTGTTCAGCGCAGTTAGCACGCCCGGACGGTTTTCGTGGATGTTCATCAGACGACGCCCACCGTGCAGTGGCAGCGAGACTTCCGGGAAGTTCACCGCAGAGAGCGTTGAGCCAt                                                             >  1:877578/1‑129 (MQ=255)                                                                                gCTCGGCGAAGATTTTGTTCAGCGCAGTTAGCACGCCCGGACGGTTTTCGTGGATGTTCATCAGACGACGCCCACCGTGCAGTGGCAGCGAGACTTCCGGGAAGTTCACCGCAGAGAGCGTTGAGCCAt                                                             <  2:877578/129‑1 (MQ=255)                                                                                gCTCGGCGAAGATTTTGTTCAGCGCAGTTAGCACGCCCGGACGGTTTTCGTGGATGTTCATCAGACGACGCCCACCGTGCAGTGGCAGCGAGACTTCCGGGAAGTTCACCGCAGAGAGCGTTGAGCCATTGTCAGAATAc                                                  >  1:148872/1‑140 (MQ=255)                                                                                gCTCGGCGAAGATTTTGTTCAGCGCAGTTAGCACGCCCGGACGGTTTTCGTGGATGTTCATCAGACGACGCCCACCGTGCAGTGGCAGCGAGACTTCCGGGAAGTTCACCGCAGAGAGCGTTGAGCCATTGTCAGAATAc                                                  <  1:1244340/140‑1 (MQ=255)                                                                                     gCGAAGATTTTGTTCAGCGCAGTTAGCACGCCCGGACGGTTTTCGTGGATGTTCATCAGACGACGCCCACCGTGCAGTGGCAGCGAGACTTCCGGGAAGTTCACCGCAGAGAGCGTTGAGCCATTGTCAGAATACTTGAt                                             <  2:122309/140‑1 (MQ=255)                                                                                         aGATTTTGTTCAGCGCAGTTAGCACGCCCGGACGGTTTTCGTGGATGTTCATCAGACGACGCCCACCGTGCAGTGGCAGCGAGACTTCCGGGAAGTTCACCGCAGAGAGCGTTGAGCCATTGTCAGAATACTTGATCAAt                                         <  2:288408/140‑1 (MQ=255)                                                                                         aGATTTTGTTCAGCGCAGTTAGCACGCCCGGACGGTTTTCGTGGATGTTCATCAGACGACGCCCACCGTGCAGTGGCAGCGAGACTTCCGGGAAGTTCACCGCAGAGAGCGTTGAGCCATTGTCAGAATACTTGATCAAt                                         >  1:1186849/1‑140 (MQ=255)                                                                                             tttGTTCAGCGCAGTTAGCACGCCCGGACGGTTTTCGTGGATGTTCATCAGACGACGCCCACCGTGCAGTGGCAGCGAGACTTCCGGGAAGTTCACCGCAGAGAGCGTTGAGCCATTGTCAGAATACTTGATCAATTTAc                                     <  2:1094439/140‑1 (MQ=255)                                                                                             tttGTTCAGCGCAGTTAGCACGCCCGGACGGTTTTCGTGGATGTTCATCAGACGACGCCCACCGTGCAGTGGCAGCGAGACTTCCGGGAAGTTCACCGCAGAGAGCGTTGAGCCATTGTCAGAATACTTGATCAATTTAc                                     <  2:551350/140‑1 (MQ=255)                                                                                                 ttCAGCGCAGTTAGCACGCCCGGACGGTTTTCGTGGATGTTCATCAGACGACGCCCACCGTGCAGTGGCAGCGAGACTTCCGGGAAGTTCACCGCAGAGAGCGTTGAGCCATTGTCAGAATACTTGATCAATTTACCCGc                                 <  2:1269599/140‑1 (MQ=255)                                                                                                 ttCAGCGCAGTTAGCACGCCCGGACGGTTTTCGTGGATGTTCATCAGACGACGCCCACCGTGCAGTGGCAGCGAGACTTCCGGGAAGTTCACCGCAGAGAGCGTTGAGCCATTGTCAGAATACTTGATCAATTTACCCGc                                 <  1:116372/140‑1 (MQ=255)                                                                                                  tCAGCGCAGTTAGCACGCCCGGACGGTTTTCGTGGATGTTCATCAGACGACGCCCACCGTGCAGTGGCAGCGAGACTTCCGGGAAGTTCACCGCAGAGAGCGTTGAGCCATTGTCAGAATACTTGATCAATTTACCCGCa                                <  1:1370088/140‑1 (MQ=255)                                                                                                         aGTTAGCACGCCCGGACGGTTTTCGTGGATGTTCATCAGACGACGCCCACCGTGCAGTGGCAGCGAGACTTCCGGGAAGTTCACCGCAGAGAGCGTTGAGCCATTGTCAGAATACTTGATCAATTTACCCGCAACTTCCa                         <  1:108648/140‑1 (MQ=255)                                                                                                         aGTTAGCACGCCCGGACGGTTTTCGTGGATGTTCATCAGACGACGCCCACCGTGCAGTGGCAGCGAGACTTCCGGGAAGTTCACCGCAGAGAGCGTTGAGCCATTGTCAGAATACTTGATCAATTTACCCGCAACTTCCa                         <  2:11801/140‑1 (MQ=255)                                                                                                            tAGCACGCCCGGACGGTTTTCGTGGATGTTCATCAGACGACGCCCACCGTGCAGTGGCAGCGAGACTTCCGGGAAGTTCACCGCAGAGAGCGTTGAGCCATTGTCAGAATACTTGATCAATTTACCCGCAACTTCCAGGc                      <  1:904484/140‑1 (MQ=255)                                                                                                            tAGCACGCCCGGACGGTTTTCGTGGATGTTCATCAGACGACGCCCACCGTGCAGTGGCAGCGAGACTTCCGGGAAGTTCACCGCAGAGAGCGTTGAGCCATTGTCAGAATACTTGATCAATTTACCCGCAACTTCCAGGc                      <  1:1141062/140‑1 (MQ=255)                                                                                                              gCACGCCCGGACGGTTTTCGTGGATGTTCATCAGACGACGCCCACCGTGCAGTGGCAGCGAGACTTCCGGGAAGTTCACCGCAGAGAGCGTTGAGCCATTGTCAGAATACTTGATCAATTTACCCGCAACTTCCAGGCCg                    <  1:951767/140‑1 (MQ=255)                                                                                                                aCGCCCGGACGGTTTTCGTGGATGTTCATCAGACGACGCCCACCGTGCAGTGGCAGCGAGACTTCCGGGAAGTTCACCGCAGAGAGCGTTGAGCCATTGTCAGAATACTTGATCAATTTACCCGCAACTTCCAGGCCGat                  >  2:141602/1‑140 (MQ=255)                                                                                                                  gCCCGGACGGTTTTCGTGGATGTTCATCAGACGACGCCCACCGTGCAGTGGCAGCGAGACTTCCGGGAAGTTCACCGCAGAGAGCGTTGAGCCATTGTCAGAATACTTGATCAATTTACCCGCAACTTCCAGGCCGatat                <  2:1186849/140‑1 (MQ=255)                                                                                                                     cGGACGGTTTTCGTGGATGTTCATCAGACGACGCCCACCGTGCAGTGGCAGCGAGACTTCCGGGAAGTTCACCGCAGAGAGCGTTGAGCCATTGTCAGAATACTTGATCAATTTACCCGCAACTTCCAGGCCGATATtct             >  1:534829/1‑140 (MQ=255)                                                                                                                      ggACGGTTTTCGTGGATGTTCATCAGACGACGCCCACCGTGCAGTGGCAGCGAGACTTCCGGGAAGTTCACCGCAGAGAGCGTTGAGCCATTGTCAGAATACTTGATCAATTTACCCGCAACTTCCAGGCCGATATtctc            >  1:240798/1‑140 (MQ=255)                                                                                                                       gACGGTTTTCGTGGATGTTCATCAGACGACGCCCACCGTGCAGTGGCAGCGAGACTTCCGGGAAGTTCACCGCAGAGAGCGTTGAGCCATTGTCAGAATACTTGATCAATTTACCCGCAACTTCCAGGCCGATATTCTcc           >  1:201585/1‑140 (MQ=255)                                                                                                                                cGTGGATGTTCATCAGACGACGCCCACCGTGCAGTGGCAGCGAGACTTCCGGGAAGTTCACCGCAGAGAGCGTTGAGCCATTGTCAGAATACTTGATCAATTTACCCGCAACTTCCAGGCCGATATTCTCCTGCGCTTcc  >  1:450703/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                   TCGTCGGCTTCAATATCAATAACCACATAACCCATCTGGGCGGAAGTTTGCAGATATTGCGCGGCGATGTTGACGCCCTGCTCGGCGAAGATTTTGTTCAGCGCAGTTAGCACGCCCGGACGGTTTTCGTGGATGTGCATCAGACGACGCCCACCGTGCAGTGGCAGCGAGACTTCCGGGAAGTTCACCGCAGAGAGCGTTGAGCCATTGTCAGAATACTTGATCAATTTACCCGCAACTTCCAGGCCGATATTCTCCTGCGCTTCC  >  NC_000913/3057251‑3057517

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑