Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,075,476	T→A	Q236L (CAG→CTG)	yggF ←	fructose 1,6‑bisphosphatase YggF

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,075,476	0	T	A	100.0%	283.7 / NA	81	Q236L (CAG→CTG)	yggF	fructose 1,6‑bisphosphatase YggF
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (40/41);  total (40/41)

AGCGCTAAAGAGGATATCGTTCCCCCTCACCAGTTCATCGAGCGAGTACACACGGTTAACGTCAACGCCCATTGCCTTGCAACGCTTGCGCTCCTGCTCTGCAATCTGCCGATTTTCCGTGTAATCCCCTTTAGCCTGGCAAAAGTCAATCAGCTCGGCCTGCATATCCCCGCCTAATGCTTTAACGGCACAGGCAGAAATCACGCCTTCTGGCGCACCGCCGATGGTGTACATCACATCGTAAGGATTATCCTGCCAGCAGGTCAGCACG  >  NC_000913/3075340‑3075610                                                                                                                                         |                                                                                                                                       aGCGCTAAAGAGGATATCGTTCCCCCTCACCAGTTCATCGAGCGAGTACACACGGTTAACGTCAACGCCCATTGCCTTGCAACGCTTGCGCTCCTGCTCTGCAATCTGCCGATTTTCCGTGTAATCCCCTTTAGCCAGGc                                                                                                                                     <  1:1008179/140‑1 (MQ=255)     cTAAAGAGGATATCGTTCCCCCTCACCAGTTCATCGAGCGAGTACACACGGTTAACGTCAACGCCCATTGCCTTGCAACGCTTGCGCTCCTGCTCTGCAATCTGCCGATTTTCCGTGTAATCCCCTTTAGCCAGGCaaaa                                                                                                                                 <  1:1013451/140‑1 (MQ=255)     cTAAAGAGGATATCGTTCCCCCTCACCAGTTCATCGAGCGAGTACACACGGTTAACGTCAACGCCCATTGCCTTGCAACGCTTGCGCTCCTGCTCTGCAATCTGCCGATTTTCCGTGTAATCCCCTTTAGCCAGGCaaaa                                                                                                                                 >  1:1356656/1‑140 (MQ=255)        aaGAGGATATCGTTCCCCCTCACCAGTTCATCGAGCGAGTACACACGGTTAACGTCAACGCCCATTGCCTTGCAACGCTTGCGCTCCTGCTCTGCAATCTGCCGATTTTCCGTGTAATCCCCTTTAGCCAGGCAAAAGTc                                                                                                                              >  2:80943/1‑140 (MQ=255)          gagGATATCGTTCCCCCTCACCAGTTCATCGAGCGAGTACACACGGTTAACGTCAACGCCCATTGCCTTGCAACGCTTGCGCTCCTGCTCTGCAATCTGCCGATTTTCCGTGTAATCCCCTTTAGCCAGGCAAAAGTCaa                                                                                                                            <  1:513543/140‑1 (MQ=255)             gATATCGTTCCCCCTCACCAGTTCATCGAGCGAGTACACACGGTTAACGTCAACGCCCATTGCCTTGCAACGCTTGCGCTCCTGCTCTGCAATCTGCCGATTTTCCGTGTAATCCCCTTTAGCCAGGCAAAAGTCAATCa                                                                                                                         >  1:843859/1‑140 (MQ=255)             gATATCGTTCCCCCTCACCAGTTCATCGAGCGAGTACACACGGTTAACGTCAACGCCCATTGCCTTGCAACGCTTGCGCTCCTGCTCTGCAATCTGCCGATTTTCCGTGTAATCCCCTTTAGCCAGGCAAAAGTCAATCa                                                                                                                         >  2:834603/1‑140 (MQ=255)               tatCGTTCCCCCTCACCAGTTCATCGAGCGAGTACACACGGTTAACGTCAACGCCCATTGCCTTGCAACGCTTGCGCTCCTGCTCTGCAATCTGCCGATTTTCCGTGTAATCCCCTTTAGCCAGGCAAAAGTCAATCAGc                                                                                                                       <  1:857318/140‑1 (MQ=255)               tatCGTTCCCCCTCACCAGTTCATCGAGCGAGTACACACGGTTAACGTCAACGCCCATTGCCTTGCAACGCTTGCGCTCCTGCTCTGCAATCTGCCGATTTTCCGTGTAATCCCCTTTAGCCAGGCAAAAGTCAATCAGc                                                                                                                       <  1:802681/140‑1 (MQ=255)               tatCGTTCCCCCTCACCAGTTCATCGAGCGAGTACACACGGTTAACGTCAACGCCCATTGCCTTGCAACGCTTGCGCTCCTGCTCTGCAATCTGCCGATTTTCCGTGTAATCCCCTTTAGCCAGGCAAAAGTCAATCAGc                                                                                                                       <  1:414915/140‑1 (MQ=255)               tatCGTTCCCCCTCACCAGTTCATCGAGCGAGTACACACGGTTAACGTCAACGCCCATTGCCTTGCAACGCTTGCGCTCCTGCTCTGCAATCTGCCGATTTTCCGTGTAATCCCCTTTAGCCAGGCAAAAGTCAATCAGc                                                                                                                       <  1:834603/140‑1 (MQ=255)               tatCGTTCCCCCTCACCAGTTCATCGAGCGAGTACACACGGTTAACGTCAACGCCCATTGCCTTGCAACGCTTGCGCTCCTGCTCTGCAATCTGCCGATTTTCCGTGTAATCCCCTTTAGCCAGGCAAAAGTCAATCAGc                                                                                                                       <  2:1191681/140‑1 (MQ=255)                atCGTTCCCCCTCACCAGTTCATCGAGCGAGTACACACGGTTAACGTCAACGCCCATTGCCTTGCAACGCTTGCGCTCCTGCTCTGCAATCTGCCGATTTTCCGTGTAATCCCCTTTAGCCAGGCAAAAGTCAATCAGCt                                                                                                                      <  2:1102062/140‑1 (MQ=255)                atCGTTCCCCCTCACCAGTTCATCGAGCGAGTACACACGGTTAACGTCAACGCCCATTGCCTTGCAACGCTTGCGCTCCTGCTCTGCAATCTGCCGATTTTCCGTGTAATCCCCTTTAGCCAGGCAAAAGTCAATCAGCt                                                                                                                      <  1:299648/140‑1 (MQ=255)                atCGTTCCCCCTCACCAGTTCATCGAGCGAGTACACACGGTTAACGTCAACGCCCATTGCCTTGCAACGCTTGCGCTCCTGCTCTGCAATCTGCCGATTTTCCGTGTAATCCCCTTTAGCCAGGCAAAAGTCAATCAGCt                                                                                                                      <  1:6710/140‑1 (MQ=255)                atCGTTCCCCCTCACCAGTTCATCGAGCGAGTACACACGGTTAACGTCAACGCCCATTGCCTTGCAACGCTTGCGCTCCTGCTCTGCAATCTGCCGATTTTCCGTGTAATCCCCTTTAGCCAGGCAAAAGTCAATCAGCt                                                                                                                      <  1:531279/140‑1 (MQ=255)                  cGTTCCCCCTCACCAGTTCATCGAGCGAGTACACACGGTTAACGTCAACGCCCATTGCCTTGCAACGCTTGCGCTCCTGCTCTGCAATCTGCCGATTTTCCGTGTAATCCCCTTTAGCCAGGCAAAAGTCAATCAGCTCg                                                                                                                    >  1:945265/1‑140 (MQ=255)                  cGTTCCCCCTCACCAGTTCATCGAGCGAGTACACACGGTTAACGTCAACGCCCATTGCCTTGCAACGCTTGCGCTCCTGCTCTGCAATCTGCCGATTTTCCGTGTAATCCCCTTTAGCCAGGCAAAAGTCAATCAGCTCg                                                                                                                    >  2:1185041/1‑140 (MQ=255)                  cGTTCCCCCTCACCAGTTCATCGAGCGAGTACACACGGTTAACGTCAACGCCCATTGCCTTGCAACGCTTGCGCTCCTGCTCTGCAATCTGCCGATTTTCCGTGTAATCCCCTTTAGCCAGGCAAAAGTCAATCAGCTCg                                                                                                                    >  2:516727/1‑140 (MQ=255)                    ttCCCCCTCACCAGTTCATCGAGCGAGTACACACGGTTAACGTCAACGCCCATTGCCTTGCAACGCTTGCGCTCCTGCTCTGCAATCTGCCGATTTTCCGTGTAATCCCCTTTAGCCAGGCAAAAGTCAATCAGCTCGGc                                                                                                                  <  1:1010411/140‑1 (MQ=255)                    ttCCCCCTCACCAGTTCATCGAGCGAGTACACACGGTTAACGTCAACGCCCATTGCCTTGCAACGCTTGCGCTCCTGCTCTGCAATCTGCCGATTTTCCGTGTAATCCCCTTTAGCCAGGCAAAAGTCAATCAGCTCGGc                                                                                                                  <  1:979210/140‑1 (MQ=255)                    ttCCCCCTCACCAGTTCATCGAGCGAGTACACACGGTTAACGTCAACGCCCATTGCCTTGCAACGCTTGCGCTCCTGCTCTGCAATCTGCCGATTTTCCGTGTAATCCCCTTTAGCCAGGCAAAAGTCAATCAGCTCGGc                                                                                                                  <  1:819247/140‑1 (MQ=255)                    ttCCCCCTCACCAGTTCATCGAGCGAGTACACACGGTTAACGTCAACGCCCATTGCCTTGCAACGCTTGCGCTCCTGCTCTGCAATCTGCCGATTTTCCGTGTAATCCCCTTTAGCCAGGCAAAAGTCAATCAGCTCGGc                                                                                                                  <  2:1356656/140‑1 (MQ=255)                    ttCCCCCTCACCAGTTCATCGAGCGAGTACACACGGTTAACGTCAACGCCCATTGCCTTGCAACGCTTGCGCTCCTGCTCTGCAATCTGCCGATTTTCCGTGTAATCCCCTTTAGCCAGGCAAAAGTCAATCAGCTCGGc                                                                                                                  >  2:447441/1‑140 (MQ=255)                    ttCCCCCTCACCAGTTCATCGAGCGAGTACACACGGTTAACGTCAACGCCCATTGCCTTGCAACGCTTGCGCTCCTGCTCTGCAATCTGCCGATTTTCCGTGTAATCCCCTTTAGCCAGGCAAAAGTCAATCAGCTCGGc                                                                                                                  <  1:516727/140‑1 (MQ=255)                    ttCCCCCTCACCAGTTCATCGAGCGAGTACACACGGTTAACGTCAACGCCCATTGCCTTGCAACGCTTGCGCTCCTGCTCTGCAATCTGCCGATTTTCCGTGTAATCCCCTTTAGCCAGGCAAAAGTCAATCAGCTCGGc                                                                                                                  <  1:269347/140‑1 (MQ=255)                     tCCCCCTCACCAGTTCATCGAGCGAGTACACACGGTTAACGTCAACGCCCATTGCCTTGCAACGCTTGCGCTCCTGCTCTGCAATCTGCCGATTTTCCGTGTAATCCCCTTTAGCCAGGCAAAAGTCAATCAGCTCGGcc                                                                                                                 >  2:138169/1‑140 (MQ=255)                       ccccTCACCAGTTCATCGAGCGAGTACACACGGTTAACGTCAACGCCCATTGCCTTGCAACGCTTGCGCTCCTGCTCTGCAATCTGCCGATTTTCCGTGTAATCCCCTTTAGCCAGGCAAAAGTCAATCAGCTCGGCCTg                                                                                                               >  1:946255/1‑140 (MQ=255)                         ccTCCCCAGTTCATCGAGCGAGTACACGCGTTTAACGTCACCCCCCTTTGCCTTGCAACGCTTGCGCTCCTGCTCTGCAATCTGCCGTTTTTCCGTGTATCCCCCTTTAGCCAGGCAAAAGTCAATCAGCTCGGCCTGCa                                                                                                             <  2:448700/140‑1 (MQ=255)                         ccTCACCAGTTCATCGAGCGAGTACACACGGTTAACGTCAACGCCCATTGCCTTGCAACGCTTGCGCTCCTGCTCTGCAATCTGCCGATTTTCCGTGTAATCCCCTTTAGCCAGGCAAAAGTCAATCAGCTCGGCCTGCa                                                                                                             >  1:448700/1‑140 (MQ=255)                         ccTCACCAGTTCATCGAGCGAGTACACACGGTTAACGTCAACGCCCATTGCCTTGCAACGCTTGCGCTCCTGCTCTGCAATCTGCCGATTTTCCGTGTAATCCCCTTTAGCCAGGCAAAAGTCAATCAGCTCGGCCTGCa                                                                                                             >  2:473553/1‑140 (MQ=255)                             aCCAGTTCATCGAGCGAGTACACACGGTTAACGTCAACGCCCATTGCCTTGCAACGCTTGCGCTCCTGCTCTGCAATCTGCCGATTTTCCGTGTAATCCCCTTTAGCCAGGCAAAAGTCAATCAGCTCGGCCTGCATATc                                                                                                         <  1:473553/140‑1 (MQ=255)                               cAGTTCATCGAGCGAGTACACACGGTTAACGTCAACGCCCATTGCCTTGCAACGCTTGCGCTCCTGCTCTGCAATCTGCCGATTTTCCGTGTAATCCCCTTTAGCCAGGCAAAAGTCAATCAGCTCGGCCTGCATATccc                                                                                                       <  2:843859/140‑1 (MQ=255)                                 gTTCATCGAGCGAGTACACACGGTTAACGTCAACGCCCATTGCCTTGCAACGCTTGCGCTCCTGCTCTGCAATCTGCCGATTTTCCGTGTAATCCCCTTTAGCCAGGCAAAAGTCAATCAGCTCGGCCTGCATATCCCCg                                                                                                     >  2:768665/1‑140 (MQ=255)                                  ttCATCGAGCGAGTACACACGGTTAACGTCAACGCCCATTGCCTTGCAACGCTTGCGCTCCTGCTCTGCAATCTGCCGATTTTCCGTGTAATCCCCTTTAGCCAGGCAAAAGTCAATCAGCTCGGCCTGCATATCCCCGc                                                                                                    >  2:313009/1‑140 (MQ=255)                                  ttCATCGAGCGAGTACACACGGTTAACGTCAACGCCCATTGCCTTGCAACGCTTGCGCTCCTGCTCTGCAATCTGCCGATTTTCCGTGTAATCCCCTTTAGCCAGGCAAAAGTCAATCAGCTCGGCCTGCATATCCCCGc                                                                                                    >  1:500899/1‑140 (MQ=255)                                     aTCGAGCGAGTACACACGGTTAACGTCAACGCCCATTGCCTTGCAACGCTTGCGCTCCTGCTCTGCAATCTGCCGATTTTCCGTGTAATCCCCTTTAGCCAGGCAAAAGTCAATCAGCTCGGCCTGCATATCCCCGCCTa                                                                                                 >  2:498303/1‑140 (MQ=255)                                     aTCGAGCGAGTACACACGGTTAACGTCAACGCCCATTGCCTTGCAACGCTTGCGCTCCTGCTCTGCAATCTGCCGATTTTCCGTGTAATCCCCTTTAGCCAGGCAAAAGTCAATCAGCTCGGCCTGCATATCCCCGCCTa                                                                                                 <  1:723398/140‑1 (MQ=255)                                       cgagcgagTACACACGGTTAACGTCAACGCCCATTGCCTTGCAACGCTTGCGCTCCTGCTCTGCAATCTGCCGATTTTCCGTGTAATCCCCTTTAGCCAGGCAAAAGTCAATCAGCTCGGCCTGCATATCCCCGCCTAAt                                                                                               >  2:259119/1‑140 (MQ=255)                                       cgagcgagTACACACGGTTAACGTCAACGCCCATTGCCTTGCAACGCTTGCGCTCCTGCTCTGCAATCTGCCGATTTTCCGTGTAATCCCCTTTAGCCAGGCAAAAGTCAATCAGCTCGGCCTGCATATCCCCGCCTAAt                                                                                               >  2:709010/1‑140 (MQ=255)                                       cgagcgagTACACACGGTTAACGTCAACGCCCATTGCCTTGCAACGCTTGCGCTCCTGCTCTGCAATCTGCCGATTTTCCGTGTAATCCCCTTTAGCCAGGCAAAAGTCAATCAGCTCGGCCTGCATATCCCCGCCTAAt                                                                                               >  2:1146811/1‑140 (MQ=255)                                           cgagTACACACGGTTAACGTCAACGCCCATTGCCTTGCAACGCTTGCGCTCCTGCTCTGCAATCTGCCGATTTTCCGTGTAATCCCCTTTAGCCAGGCAAAAGTCAATCAGCTCGGCCTGCATATCCCCGCCTAATGCtt                                                                                           >  2:1473027/1‑140 (MQ=255)                                             agTACACACGGTTAACGTCAACGCCCATTGCCTTGCAACGCTTGCGCTCCTGCTCTGCAATCTGCCGATTTTCCGTGTAATCCCCTTTAGCCAGGCAAAAGTCAATCAGCTCGGCCTGCATATCCCCGCCTAATGCTTTa                                                                                         <  2:760174/140‑1 (MQ=255)                                                   cacGGTTAACGTCAACGCCCATTGCCTTGCAACGCTTGCGCTCCTGCTCTGCAATCTGCCGATTTTCCGTGTAATCCCCTTTAGCCAGGCAAAAGTCAATCAGCTCGGCCTGCATATCCCCGCCTAATGCTTTAACGGca                                                                                   <  2:34998/140‑1 (MQ=255)                                                        ttAACGTCAACGCCCATTGCCTTGCAACGCTTGCGCTCCTGCTCTGCAATCTGCCGATTTTCCGTGTAATCCCCTTTAGCCAGGCAAAAGTCAATCAGCTCGGCCTGCATATCCCCGCCTAATGCTTTAACGGCACAgac                                                                              >  1:1357396/1‑138 (MQ=255)                                                        ttAACGTCAACGCCCATTGCCTTGCAACGCTTGCGCTCCTGCTCTGCAATCTGCCGATTTTCCGTGTAATCCCCTTTAGCCAGGCAAAAGTCAATCAGCTCGGCCTGCATATCCCCGCCTAATGCTTTAACGGCACAGGc                                                                              >  2:1188056/1‑140 (MQ=255)                                                         tAACGTCAACGCCCATTGCCTTGCAACGCTTGCGCTCCTGCTCTGCAATCTGCCGATTTTCCGTGTAATCCCCTTTAGCCAGGCAAAAGTCAATCAGCTCGGCCTGCATATCCCCGCCTAATGCTTTAACGGca                                                                                   <  1:171372/134‑1 (MQ=255)                                                         tAACGTCAACGCCCATTGCCTTGCAACGCTTGCGCTCCTGCTCTGCAATCTGCCGATTTTCCGTGTAATCCCCTTTAGCCAGGCAAAAGTCAATCAGCTCGGCCTGCATATCCCCGCCTAATGCTTTAACGGca                                                                                   >  2:171372/1‑134 (MQ=255)                                                                aaCGCCCATTGCCTTGCAACGCTTGCGCTCCTGCTCTGCAATCTGCCGATTTTCCGTGTAATCCCCTTTAGCCAGGCAAAAGTCAATCAGCTCGGCCTGCATATCCCCGCCTAATGCTTTAACGGCACAGGCAGAAATCa                                                                      >  1:594173/1‑140 (MQ=255)                                                                aaCGCCCATTGCCTTGCAACGCTTGCGCTCCTGCTCTGCAATCTGCCGATTTTCCGTGTAATCCCCTTTAGCCAGGCAAAAGTCAATCAGCTCGGCCTGCATATCCCCGCCTAATGCTTTAACGGCACAGGCAGAAATCa                                                                      >  2:341361/1‑140 (MQ=255)                                                                       aTTGCCTTGCAACGCTTGCGCTCCTGCTCTGCAATCTGCCGATTTTCCGTGTAATCCCCTTTAGCCAGGCAAAAGTCAATCAGCTCGGCCTGCATATCCCCGCCTAATGCTTTAACGGCACAGGCAGAAATCACGCCTTc                                                               >  2:1334494/1‑140 (MQ=255)                                                                             ttGCACCGCTGGCGCTCCTGCGCGGAAATCTGCCGATTTTCCGTGAAACCCCCTTTACCAAGGAAAAATTCAATCAGCTCGCCCTGCATATCCCCGCCTAATGCTTTAACGGCAGGGGCAGAATTCACGCCTTCTGgcgc                                                         <  2:792216/140‑1 (MQ=255)                                                                                cAACGCTTGCGCTCCTGCTCTGCAATCTGCCGATTTTCCGTGTAATCCCCTTTAGCCAGGCAAAAGTCAATCAGCTCGGCCTGCATATCCCCGCCTAATGCTTTAACGGCACAGGCAGAAATCACGCCTTCTGGCGCAcc                                                      >  2:731254/1‑140 (MQ=255)                                                                                  aCGCTTGCGCCCCTGCTCTGCAATCTGCCGATTTTCCGTGTAATCCCCTTTAGCCAGGCAAAAGTCAATCAGCTCGGCCTGCATATCCCCGCCTAATGCTTTAACGGCACAGGCAGAAATCACGCCTTCTGGCGCAccgc                                                    <  1:1174012/140‑1 (MQ=255)                                                                                    gCTTGCGCTCCTGCTCTGCAATCTGCCGATTTTCCGTGTAATCCCCTTTAGCCAGCCAAAAGTCAATCAGCTCGGCCTGCATATCCCCGCCTAATGCTTTAACGGCACAGGCAGAAATCACGCCTTCTGGCGCAccgccg                                                  <  2:1122811/140‑1 (MQ=255)                                                                                     cTTGCGCTCCTGCTCTGCAATCTGCCGATTTTCCGTGTAATCCCCTTTAGCCAGGCAAAAGTCAATCAGCTCGGCCTGCATATCCCCGCCTAATGCTTTAACGGCACAGGCAGAAATCACGCCTTCTGGCGCACCGCCGa                                                 <  1:138169/140‑1 (MQ=255)                                                                                     cTTGCGCTCCTGCTCTGCAATCTGCCGATTTTCCGTGTAATCCCCTTTAGCCAGGCAAAAGTCAATCAGCTCGGCCTGCATATCCCCGCCTAATGCTTTAACGGCACAGGCAGAAATCACGCCTTCTGGCGCACCGCCGa                                                 <  1:1188056/140‑1 (MQ=255)                                                                                     cTTGCGCTCCTGCTCTGCAATCTGCCGATTTTCCGTGTAATCCCCTTTAGCCAGGCAAAAGTCAATCAGCTCGGCCTGCATATCCCCGCCTAATGCTTTAACGGCACAGGCAGAAATCACGCCTTCTGGCGCACCGCCGa                                                 <  1:731254/140‑1 (MQ=255)                                                                                     cTTGCGCTCCTGCTCTGCAATCTGCCGATTTTCCGTGTAATCCCCTTTAGCCAGGCAAAAGTCAATCAGCTCGGCCTGCATATCCCCGCCTAATGCTTTAACGGCACAGGCAGAAATCACGCCTTCTGGCGCACCGCCGa                                                 >  1:1121958/1‑140 (MQ=255)                                                                                     cTTGCGCTCCTGCTCTGCAATCTGCCGATTTTCCGTGTAATCCCCTTTAGCCAGGCAAAAGTCAATCAGCTCGGCCTGCATATCCCCGCCTAATGCTTTAACGGCACAGGCAGAAATCACGCCTTCTGGCGCACCGCCGa                                                 >  1:995539/1‑140 (MQ=255)                                                                                       tGCGCTCCTGCTCTGCAATCTGCCGATTTTCCGTGTAATCCCCTTTAGCCAGGCAAAAGTCAATCAGCTCGGCCTGCATATCCCCGCCTAATGCTTTAACGGCACAGGCAGAAATCACGCCTTCTGGCGCACCGCCGATg                                               <  1:80943/140‑1 (MQ=255)                                                                                        gcgcTCCTGCTCTGCAATCTGCCGATTTTCCGTGTAACCCCCTTTAGCCAGGCAAAAGTCAATCAGCTCGGCCTGCATATCCCCGCCTAATGCTTTAACGGCACGGGAAGAAATCACGCCTTCTGGCGCACCGCCGATgg                                              <  2:677173/140‑1 (MQ=255)                                                                                         cgttCCTGCTCTGCATTCTGCCGATTTTCCGTGTAACCCCCTTTAGCGAGGCAAAAGTCAATCAGCTCGGCCTGCATATCCCCGCCTAATGCTTTAACGGCACATGCAGAAATCACGCCTTCTGGCGCTCCGCCGATGgt                                             <  2:629662/137‑1 (MQ=255)                                                                                         cgcTCCTGCTCTGCAATCTGCCGATTTTCCGTGTAATCCCCTTTAGCCAGGCAAAAGTCAATCAGCTCGGCCTGCATATCCCCGCCTAATGCTTTAACGGCACAGGCAGAAATCACGCCTTCTGGCGCACCGCCGATGgt                                             <  2:1176447/140‑1 (MQ=255)                                                                                         cgcTCCTGCTCTGCAATCTGCCGATTTTCCGTGTAATCCCCTTTAGCCAGGCAAAAGTCAATCAGCTCGGCCTGCATATCCCCGCCTAATGCTTTAACGGCACAGGCAGAAATCACGCCTTCTGGCGCACCGCCGATGgt                                             <  1:67356/140‑1 (MQ=255)                                                                                         cgcTCCTGCTCTGCAATCTGCCGATTTTCCGTGTAATCCCCTTTAGCCAGGCAAAAGTAAATCAGCTCGGCCTGCATATCCCCGCCTAATGCTTTAACGGCACAGGCAGAAATCACGCCTTCTGGCGCACCGCCGATGgt                                             <  1:205110/140‑1 (MQ=255)                                                                                           cTCCTGCTCTGCAATCTGCCGATTTTCCGTGTAATCCCCTTTAGCCAGGCAAAAGTCAATCAGCTCGGCCTGCATATCCCCGCCTAATGCTTTAACGGCCCAGGCAGAAATCACGCCTTCTGGCGCACCGCCGATGgtgt                                           <  1:219976/140‑1 (MQ=255)                                                                                                gCTCTGCAATCTGCCGATTTTCCGTGTAATCCCCTTTAGCCAGGCAAAAGTCAATCAGCTCGGCCTGCATATCCCCGCCTAATGCTTTAACGGCACAGGCAGAAATCACGCCTTCTGGCGCACCGCCGATGGTGTacatc                                      <  1:259119/140‑1 (MQ=255)                                                                                                  tctGCAATCTGCCGTTTTTCCGTGTAATCCCCTTAAGCGAGGAAAAAGTCAATCACCTCGGCCTGCATATCCCCGCCAAATGCTTTAACGGCCCAGGCAGAAATCAGGCTTTCTGGCGCTCCGCCGATGGTGTacatcac                                    <  2:399782/140‑1 (MQ=255)                                                                                                  tctGCAATCTGCCGATTTTCCGTGTAATCCCCTTTAGCCAGGCAAAAGTCAATCAGCTCGGCCTGCATATCCCCGCCTAATGCTTTAACGGCACAGGCAGAAATCACGCCTTCTGGCGCACCGCCGATGGTGTacatcac                                    <  1:768665/140‑1 (MQ=255)                                                                                                  tctGCAATCTGCCGATTTTCCGTGTAATCCCCTTTAGCCAGGCAAAAGTCAATCAGCTCGGCCTGCATATCCCCGCCTAATGCTTTAACGGCACAGGCAGAAATCACGCCTTCTGGCGCACCGCCGATGGTGTacatcac                                    <  1:447441/140‑1 (MQ=255)                                                                                                   ctGCAATCTGCCGATTTTCCGTGTAATCCCCTTTAGCCAGGCAAAAGTCAATCAGCTCGGCCTGCATATCCCCGCCTAATGCTTTAACGGCACAGGCAGAAATCACGCCTTCTGGCGCACCGCCGATGGTGTacatcaca                                   <  1:1146811/140‑1 (MQ=255)                                                                                                   ctGCAATCTCCCGATTTTCCGTGTAATCCCCTTTATCCAGGCAAAAGTCAAGCAGCTCGGCCTGCAGCTCCCCTCCTAATGCTTTAACGGCACGGGCAAAAAACACGCTTGCTGGCGCGCCGCCGATGGTGTacatcgca                                   <  2:801851/140‑1 (MQ=255)                                                                                                    tGCAATCTGCCGATTTTCCGTGTAATCCCCTTTAGCCAGGCAAAAGTCAATCAGCTCGGCCTGCATATCCCCGCCTAATGCTTTAACGGCACAGGCAGAAATCACGCCTTCTGGCGCACCGCCGATGgt                                             <  1:7844/129‑1 (MQ=255)                                                                                                    tGCAATCTGCCGATTTTCCGTGTAATCCCCTTTAGCCAGGCAAAAGTCAATCAGCTCGGCCTGCATATCCCCGCCTAATGCTTTAACGGCACAGGCAGAAATCACGCCTTCTGGCGCACCGCCGATGgt                                             >  2:7844/1‑129 (MQ=255)                                                                                                    tGCAATCTGCCGATTTTCCGTGTAATCCCCTTTAGCCAGGCAAAAGTCAATCAGCTCGGCCTGCATATCCCCGCCTAATGCTTTAACGGCACAGGCAGAAATCACGCCTTCTGGCGCACCGCCGATGGTGTacatcacat                                  >  2:1264500/1‑140 (MQ=255)                                                                                                      cAATCTGCCGATTTTCCGTGTAATCCCCTTTAGCCAGGCAAAAGTCAATCAGCTCGGCCTGCATATCCCCGCCTAATGCTTTAACGGCACAGGCAGAAATCACGCCTTCTGGCGCACCGCCGATGGCGTACATCACATCg                                >  2:1076597/1‑140 (MQ=255)                                                                                                           tGCCGATTTTCCGTGTAATCCCCTTTAGCCAGGCAAAAGTCAATCAGCTCGGCCTGCATATCCCCGCCTAATGCTTTAACGGCACAGGCAGAAATCACGCCTTCTGGCGCACCGCCGATGGTGTACATCACATCGTAAgg                           >  1:971135/1‑140 (MQ=255)                                                                                                           tGCCGATTTTCCGTGTAATCCCCTTTAGCCAGGCAAAAGTCAATCAGCTCGGCCTGCATATCCCCGCCTAATGCTTTAACGGCACAGGCAGAAATCACGCCTTCTGGCGCACCGCCGATGGTGTACATCACATCGTAAgg                           >  2:764251/1‑140 (MQ=255)                                                                                                           tGCCGATTTTCCGTGTAATCCCCTTTAGCCAGGCAAAAGTCAATCAGCTCGGCCTGCATATCCCCGCCTAATGCTTTAACGGCACAGGCAGAAATCACGCCTTCTGGCGCACCGCCGATGGTGTACATCACATCGTAAgg                           >  1:424966/1‑140 (MQ=255)                                                                                                             ccGATTTTCCGTGTAATCCCCTTTAGCCAGGCAAAAGTCAATCAGCTCGGCCTGCATATCCCCGCCTAATGCTTTAACGGCACAGGCAGAAATCACGCCTTCTGGCGCACCGCCGATGGTGTACATCACATCGTAAGGAt                         >  2:509457/1‑140 (MQ=255)                                                                                                              cGATTTTCCGTGTAATCCCCTTTAGCCAGGCAAAAGTCAATCAGCTCGGCCTGCATATCCCCGCCTAATGCTTTAACGGCACAGGCAGAAATCACCCCTTCGGGCCCGCCGCCGATGGTGTACATCACATCGTAAGGAtt                        <  2:946255/140‑1 (MQ=255)                                                                                                                aTTTTCCGTGTAATCCCCTTTAGCCAGGCAAAAGTCAATCAGCTCGGCCTGCATATCCCCGCCTAATGCTTTAACGGCACAGGCAGAAATCACGCCTTCTGGCGCACCGCCGATGGTGTACATCACATCGTAAGGATTAt                      >  1:707892/1‑140 (MQ=255)                                                                                                                           aaTCCCCTTTAGCCAGGCAAAAGTCAATCAGCTCGGCCTGCATATCCCCGCCTAATGCTTTAACGGCACAGGCAGAAATCACGCCTTCTGGCGCACCGCCGATGGTGTACATCACATCGTAAGGATTATCCTGCcagcag           >  1:62035/1‑140 (MQ=255)                                                                                                                             tCCCCTTTAGCCAGGCAAAAGTCAATCAGCTCGGCCTGCATATCCCCGCCTAATGCTTTAACGGCACAGGCAGAAATCACGCCTTCTGGCGCACCGCCGATGGTGTACATCACATCGTAAGGATTATCCTGCCAGCAGGt         >  1:217160/1‑140 (MQ=255)                                                                                                                                  tttAGCCAGGCAAAAGTCAATCAGCTCGGCCTGCATATCCCCGCCTAATGCTTTAACGGCACAGGCAGAAATCACGCCTTCTGGCGCACCGCCGATGGTGTACATCACATCGTAAGGATTATCCTGCCAGCAGGTCAGCa    >  2:14092/1‑140 (MQ=255)                                                                                                                                    tAGCCAGGCAAAAGTCAATCAGCTCGGCCTGCATATCCCCGCCTAATGCTTTAACGGCACAGGCAGAAATCACGCCTTCTGGCGCACCGCCGATGGTGTACATCACATCGTAAGGATTATCCTGCCAGCAGGTCAGCACg  <  2:62035/140‑1 (MQ=255)                                                                                                                                    tAGCCAGGCAAAAGTCAATCAGCTCGGCCTGCATATCCCCGCCTAATGCTTTAACGGCACAGGCAGAAATCACGCCTTCTGGCGCACCGCCGATGGTGTACATCACATCGTAAGGATTATCCTGCCAGCAGGTCAGCACg  <  1:1334494/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                                       AGCGCTAAAGAGGATATCGTTCCCCCTCACCAGTTCATCGAGCGAGTACACACGGTTAACGTCAACGCCCATTGCCTTGCAACGCTTGCGCTCCTGCTCTGCAATCTGCCGATTTTCCGTGTAATCCCCTTTAGCCTGGCAAAAGTCAATCAGCTCGGCCTGCATATCCCCGCCTAATGCTTTAACGGCACAGGCAGAAATCACGCCTTCTGGCGCACCGCCGATGGTGTACATCACATCGTAAGGATTATCCTGCCAGCAGGTCAGCACG  >  NC_000913/3075340‑3075610

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑