Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,332,531	G→T	P69T (CCA→ACA)	yhbE ←	inner membrane protein YhbE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,332,531	0	G	T	100.0%	241.4 / NA	69	P69T (CCA→ACA)	yhbE	inner membrane protein YhbE
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (29/40);  total (29/40)

AACCATCATGCCAACTGGCGAGAGTTGCCCAATCACCTGCGAAGCGGTCGGACTCAGGTATTGCAAGGATGAGCTGAACAGGATGAAGTTCCCAAACAGCCCGGCGGTCGCCACTGCCAACAAAATCAACCAGCGTGGCTTACGAAACACGCGTAATGGCGGCAACCTCTTCTTCACCGCAAGAATGGCACCCAGGCCAATACTCGCCATCAAGAAACGGTAAAACACGATTGTCGGAGGTTCCATCACCTCCAGCACCTGCTTCATTGCGAT  >  NC_000913/3332394‑3332666                                                                                                                                          |                                                                                                                                        aaCCATCATGCCAACTGGCGAGAGTTGCCCAATCACCTGCGAAGCGGTCGGACTCAGGTATTGCAAGGATGAGCTGAACAGGATGAAGTTCCCAAACAGCCCGGCGGTCGCCACTGCCAACAAAATCAACCAGCGTGTCt                                                                                                                                       >  1:1353697/1‑140 (MQ=255)     atcatGCCAACTGGCGAGAGTTGCCCAATCACCTGCGAAGCGGTCGGACTCAGGTATTGCAAGGATGAGCTGAACAGGATGAAGTTCCCAAACAGCCCGGCGGTCGCCACTGCCAACAAAATCAACCAGCGTGTCTTACg                                                                                                                                   >  1:909319/1‑140 (MQ=255)         tGCCAACTGGCGAGAGTTGCCCAATCACCTGCGAAGCGGTCGGACTCAGGTATTGCAAGGATGAGCTGAACAGGATGAAGTTCCCAAACAGCCCGGCGGTCGCCACTGCCAACAAAATCAACCAGCGTGTCTTACGAAac                                                                                                                               >  2:1042957/1‑140 (MQ=255)                   cGAGAGTTGCCCAATCACCTGCGAAGCGGTCGGACTCAGGTATTGCAAGGATGAGCTGAACAGGATGAAGTTCCCAAACAGCCCGGCGGTCGCCACTGCCAACAAAATCAACCAGCGTGTCTTACGAAACACGCGTAATg                                                                                                                     >  1:1371896/1‑140 (MQ=255)                   cGAGAGTTGCCCAATCACCTGCGAAGCGGTCGGACTCAGGTATTGCAAGGATGAGCTGAACAGGATGAAGTTCCCAAACAGCCCGGCGGTCGCCACTGCCAACAAAATCAACCAGCGTGTCTTACGAAACACGCGTAATg                                                                                                                     >  1:1459816/1‑140 (MQ=255)                    gagaGTTGCCCAATCACCTGCGAAGCGGTCGGACTCAGGTATTGCAAGGATGAGCTGAACAGGATGAAGTTCCCAAACAGCCCGGCGGTCGCCACTGCCAACAAAATCAACCAGCGTGTCTTACGAAACACGCGTAATgg                                                                                                                    >  1:1035222/1‑140 (MQ=255)                     agagTTGCCCAATCACCTGCGAAGCGGTCGGACTCAGGTATTGCAAGGATGAGC‑GAACAGGATGAAGTTCCCAAACAGCCCGGCGGTCGCCACTGCCAACAAAATCAACCAGCGTGTCTTACGAAACACGCGTAATggcg                                                                                                                  <  1:743221/140‑1 (MQ=255)                      gagTTGCCCAATCACCTGCGAAGCGGTCGGACTCAGGTATTGCAAGGATGAGCTGAACAGGATGAAGTTCCCAAACAGCCCGGCGGTCGCCACTGCCAACAAAATCAACCAGCGTGTCTTACGAAACACGCGTAATggcg                                                                                                                  <  2:1156002/140‑1 (MQ=255)                      gagTTGCCCAATCACCTGCGAAGCGGTCGGACTCAGGTATTGCAAGGATGAGCTGAACAGGATGAAGTTCCCAAACAGCCCGGCGGTCGCCACTGCCAACAAAATCAACCAGCGTGTCTTACGAAACACGCGTAATggcg                                                                                                                  <  2:670984/140‑1 (MQ=255)                        gTTGCCCAATCACCTGCGAAGCGGTCGGACTCAGGTATTGCAAGGATGAGCTGAACAGGATGAAGTTCCCAAACAGCCCGGCGGTCGCCACTGCCAACAAAATCAACCAGCGTGTCTTACGAAACACGCGTAATggcggc                                                                                                                <  1:306212/140‑1 (MQ=255)                         ttGCCCAATCACCTGCGAAGCGGTCGGACTCAGGTATTGCAAGGATGAGCTGAACAGGATGAAGTTCCCAAACAGCCCGGCGGTCGCCACTGCCAACAAAATCAACCAGCGTGTCTTACGAAACACGCGTAATGGCGGCa                                                                                                               <  2:918929/140‑1 (MQ=255)                         ttGCCCAATCACCTGCGAAGCGGTCGGACTCAGGTATTGCAAGGATGAGCTGAACAGGATGAAGTTCCCAAACAGCCCGGCGGTCGCCACTGCCAACAAAATCAACCAGCGTGTCTTACGAAACACGCGTAATGGCGGCa                                                                                                               <  2:501032/140‑1 (MQ=255)                          tGCCCAATCACCTGCGAAGCGGTCGGACTCAGGTATTGCAAGGATGAGCTGAACAGGATGAAGTTCCCAAACAGCCCGGCGGTCGCCACTGCCAACAAAATCAACCAGCGTGTCTTACGAAACACGCGTAATGGCGGCaa                                                                                                              >  1:1203040/1‑140 (MQ=255)                            cccAATCACCTGCGAAGCGGTCGGACTCAGGTATTGCAAGGATGAGCTGAACAGGATGAAGTTCCCAAACAGCCCGGCGGTCGCCACTGCCAACAAAATCAACCAGCGTGTCTTACGAAACACGCGTAATGGCGGCAAcc                                                                                                            >  2:45267/1‑140 (MQ=255)                                   aCCTGCGAAGCGGTCGGACTCAGGTATTGCAAGGATGAGCTGAACAGGATGAAGTTCCCAAACAGCCCGGCGGTCGCCACTGCCAACAAAATCAACCAGCGTGTCTTACGAAACACGCGTAATGGCGGCAACCTcttctt                                                                                                     <  1:1017688/140‑1 (MQ=255)                                         gAAGCGGTCGGACTCAGGTATTGCAAGGATGAGCTGAACAGGATGAAGTTCCCAAACAGCCCGGCGGTCGCCACTGCCAACAAAATCAACCAGCGTGTCTTACGAAACACGCGTAATGGCGGCAACCTCTTCTTCACCGc                                                                                               <  1:1042957/140‑1 (MQ=255)                                           aGCGGTCGGACTCAGGTATTGCAAGGATGAGCTGAACAGGATGAAGTTCCCAAACAGCCCGGCGGTCGCCACTGCCAACAAAATCAACCAGCGTGTCTTACGAAACACGCGTAATGGCGGCAACCTCTTCTTCACCGCaa                                                                                             <  2:450100/140‑1 (MQ=255)                                               gTCGGACTCAGGTATTGCAAGGATGAGCTGAACAGGATGAAGTTCCCAAACAGCCCGGCGGTCGCCACTGCCAACAAAATCAACCAGCGTGTCTTACGAAACACGCGTAATGGCGGCAACCTCTTCTTCACCGCAAGAAt                                                                                         >  2:289437/1‑140 (MQ=255)                                               gTCGGACTCAGGTATTGCAAGGATGAGCTGAACAGGATGAAGTTCCCAAACAGCCCGGCGGTCGCCACTGCCAACAAAATCAACCAGCGTGTCTTACGAAACACGCGTAATGGCGGCAACCTCTTCTTCACCGCAAGAAt                                                                                         <  1:731325/140‑1 (MQ=255)                                               gTCGGACTCAGGTATTGCAAGGATGAGCTGAACAGGATGAAGTTCCCAAACAGCCCGGCGGTCGCCACTGCCAACAAAATCAACCAGCGTGTCTTACGAAACACGCGTAATGGCGGCAACCTCTTCTTCACCGCAAGAAt                                                                                         >  1:1286282/1‑140 (MQ=255)                                                tCGGACTCAGGTATTGCAAGGATGAGCTGAACAGGATGAAGTTCCCAAACAGCCCGGCGGTCGCCACTGCCAACAAAATCAACCAGCGTGTCTTACGAAACACGCGTAATGGCGGCAACCTCTTCTTCACCGCAAGAATg                                                                                        <  1:289437/140‑1 (MQ=255)                                                tCGGACTCAGGTATTGCAAGGATGAGCTGAACAGGATGAAGTTCCCAAACAGCCCGGCGGTCGCCACTGCCAACAAAATCAACCAGCGTGTCTTACGAAACACGCGTAATGGCGGCAACCTCTTCTTCACCGCAAGAATg                                                                                        <  1:353279/140‑1 (MQ=255)                                                tCGGACTCAGGTATTGCAAGGATGAGCTGAACAGGATGAAGTTCCCAAACAGCCCGGCGGTCGCCACTGCCAACAAAATCAACCAGCGTGTCTTACGAAACACGCGTAATGGCGGCAACCTCTTCTTCACCGCAAGAATg                                                                                        <  2:393904/140‑1 (MQ=255)                                                tCGGACTCAGGTATTGCAAGGATGAGCTGAACAGGATGAAGTTCCCAAACAGCCCGGCGGTCGCCACTGCCAACAAAATCAACCAGCGTGTCTTACGAAACACGCGTAATGGCGGCAACCTCTTCTTCACCGCAAGAATg                                                                                        <  1:682749/140‑1 (MQ=255)                                                     cTCAGGTATTGCAAGGATGAGCTGAACAGGATGAAGTTCCCAAACAGCCCGGCGGTCGCCACTGCCAACAAAATCAACCAGCGTGTCTTACGAAACACGCGTAATGGCGGCAACCTCTTCTTCACCGCAAGAATGGCAcc                                                                                   <  2:795546/140‑1 (MQ=255)                                                     cTCAGGTATTGCAAGGATGAGCTGAACAGGATGAAGTTCCCAAACAGCCCGGCGGTCGCCACTGCCAACAAAATCAACCAGCGTGTCTTACGAAACACGCGTAATGGCGGCAACCTCTTCTTCACCGCAAGAATGGCAcc                                                                                   <  2:122329/140‑1 (MQ=255)                                                     cTCAGGTATTGCAAGGATGAGCTGAACAGGATGAAGTTCCCAAACAGCCCGGCGGTCGCCACTGCCAACAAAATCAACCAGCGTGTCTTACGAAACACGCGTAATGGCGGCAACCTCTTCTTCACCGCAAGAATGGCAcc                                                                                   <  2:1227785/140‑1 (MQ=255)                                                     cTCAGGTATTGCAAGGATGAGCTGAACAGGATGAAGTTCCCAAACAGCCCGGCGGTCGCCACTGCCAACAAAATCAACCAGCGTGTCTTACGAAACACGCGTAATGGCGGCAACCTCTTCTTCACCGCAAGAATGGCAcc                                                                                   <  1:997471/140‑1 (MQ=255)                                                     cTCAGGTATTGCAAGGATGAGCTGAACAGGATGAAGTTCCCAAACAGCCCGGCGGTCGCCACTGCCAACAAAATCAACCAGCGTGTCTTACGAAACACGCGTAATGGCGGCAACCTCTTCTTCACCGCAAGAATGGCAcc                                                                                   <  1:909879/140‑1 (MQ=255)                                                     cTCAGGTATTGCAAGGATGAGCTGAACAGGATGAAGTTCCCAAACAGCCCGGCGGTCGCCACTGCCAACAAAATCAACCAGCGTGTCTTACGAAACACGCGTAATGGCGGCAACCTCTTCTTCACCGCAAGAATGGCAcc                                                                                   <  2:938546/140‑1 (MQ=255)                                                     cTCAGGTATTGCAAGGATGAGCTGAACAGGATGAAGTTCCCAAACAGCCCGGCGGTCGCCACTGCCAACAAAATCAACCAGCGTGTCTTACGAAACACGCGTAATGGCGGCAACCTCTTCTTCACCGCAAGAATGGCAcc                                                                                   <  1:865504/140‑1 (MQ=255)                                                     cTCAGGTATTGCAAGGATGAGCTGAACAGGATGAAGTTCCCAAACAGCCCGGCGGTCGCCACTGCCAACAAAATCAACCAGCGTGTCTTACGAAACACGCGTAATGGCGGCAACCTCTTCTTCACCGCAAGAATGGCAcc                                                                                   <  2:1470558/140‑1 (MQ=255)                                                     cTCAGGTATTGCAAGGATGAGCTGAACAGGATGAAGTTCCCAAACAGCCCGGCGGTCGCCACTGCCAACAAAATCAACCAGCGTGTCTTACGAAACACGCGTAATGGCGGCAACCTCTTCTTCACCGCAAGAATGGCAcc                                                                                   <  1:45267/140‑1 (MQ=255)                                                     cTCAGGTATTGCAAGGATGAGCTGAACAGGATGAAGTTCCCAAACAGCCCGGCGGTCGCCACTGCCAACAAAATCAACCAGCGTGTCTTACGAAACACGCGTAATGGCGGCAACCTCTTCTTCACCGCAAGAATGGCAcc                                                                                   <  2:909319/140‑1 (MQ=255)                                                             ttGCAAGGATGAGCTGAACAGGATGAAGTTCCCAAACAGCCCGGCGGTCGCCACTGCCAACAAAATCAACCAGCGTGTCTTACGAAACACGCGTAATGGCGGCAACCTCTTCTTCACCGCAAGAATGGCACCCAGGCCaa                                                                           >  1:1290932/1‑140 (MQ=255)                                                              tGCAAGGATGAGCTGAACAGGATGAAGTTCCCAAACAGCCCGGCGGTCGCCACTGCCAACAAAATCAACCAGCGTGTCTTACGAAACACGCGTAATGGCGGCAACCTCTTCTTCACCGCAAGAATGGCAcc                                                                                   <  1:580508/131‑1 (MQ=255)                                                              tGCAAGGATGAGCTGAACAGGATGAAGTTCCCAAACAGCCCGGCGGTCGCCACTGCCAACAAAATCAACCAGCGTGTCTTACGAAACACGCGTAATGGCGGCAACCTCTTCTTCACCGCAAGAATGGCAcc                                                                                   >  2:580508/1‑131 (MQ=255)                                                                cAAGGATGAGCTGAACAGGATGAAGTTCCCAAACAGCCCGGCGGTCGCCACTGCCAACAAAATCAAACGGCGTGTCTTACGAAACACGCGTAATGGCGGCAACCTCTTCTTCACCCCAAAAAAGGCACCCAGGCCAATAc                                                                        >  2:1065165/1‑140 (MQ=255)                                                                  aGGATGAGCTGAACAGGATGAAGTTCCCAAACAGCCCGGCGGTCGCCACTGCCAACAAAATCAACCAGCGTGTCTTACGAAACACGCGTAATGGCGGCAACCTCTTCTTTCCCGCAAAAATGGCACCCAGGCCCATACTc                                                                      >  2:344809/1‑140 (MQ=255)                                                                  aGGATGAGCTGAACAGGATGAAGTTCCCAAACAGCCCGGCGGTCGCCACTGCCAACAAAATCAACCAGCGTGTCTTACGAAACACGCGTAATGGCGGCAACCTCTTCTTCACCGCAAGAATGGCACCCAGGCCAATACTc                                                                      >  2:1484161/1‑140 (MQ=255)                                                                    gATGAGCTGAACAGGATGAAGTTCCCAAACAGCCCGGCGGTCGCCACTGCCAACAAAATCAACCAGCGTGTCTTACGAAACACGCGTAATGGCGGCAACCTCTTCTTCACCGCAAGAATGGCACCCAGGCCAATACTCGc                                                                    >  1:1021892/1‑140 (MQ=255)                                                                     aTGAGCTGAACAGGATGAAGTTCCCAAACAGCCCGGCGGACGCCACTGCCAACAAAATCAACCAGCGTGTCTTACGAAACACGCGTAATGGCGGCAACCTCTTTTTCACCGCAAAAATGGCACCCAGGCCAATAATCGcc                                                                   >  2:217518/1‑140 (MQ=255)                                                                        aGCTGAACAGGATGAAGTTCCCAAACAGCCCGGCGGTCGCCACTGCCAACAAAATCAACCAGCGTGTCTTACGAAACACGCGTAATGGCGGCAACCTCTTCTTCACCGCAAGAATGGCACCCAGGCCAATACTCGCCATc                                                                >  2:1360880/1‑140 (MQ=255)                                                                               cAGGATGAAGTTCCCAAACAGCCCGGCGGTCGCCACTGCCAACAAAATCAACCAGCGTGTCTTACGAAACACGCGTAATGGCGGCAACCTCTTCTTCACCGCAAGAATGGCACCCCGGCCAATACTCGCCATCAAGAAAc                                                         >  2:584264/1‑140 (MQ=255)                                                                                  gATGAAGTTCCCAAACAGCCCGGCGGTCGCCACTGCCAACAAAATCAACCAGCGTGTCTTACGAAACACGCGTAATGGCGGCAACCTCTTCTTCACCGCAAGAATGGCACCCAGGCCAATACTCGCCATCAAGaaa                                                          <  1:1194917/136‑1 (MQ=255)                                                                                  gATGAAGTTCCCAAACAGCCCGGCGGTCGCCACTGCCAACAAAATCAACCAGCGTGTCTTACGAAACACGCGTAATGGCGGCAACCTCTTCTTCACCGCAAGAATGGCACCCAGGCCAATACTCGCCATCAAGaaa                                                          >  2:1194917/1‑136 (MQ=255)                                                                                  gATGAAGTTCCCAAACAGCCCGGCGGTCGCCACTGCCAACAAAATCAACCAGCGTGTCTTACGAAACACGCGTAATGGCGGCAACCTCTTCTTCACCGCAAGAATGGCACCCAGGCCAATACTCGCCATCAAGAAACGGt                                                      >  1:261893/1‑140 (MQ=255)                                                                                   aTGAAGTTCCCAAACAGCCCGGCGGTCGCCACTGCCAACAAAATCAACCAGCGTGTCTTACGAAACACGCGTAATGGCGGCAACCTCTTCTTCACCGCAAGAATGGCACCCAGGCCAATACTCGCCATCAAGAAACGGTa                                                     <  2:281460/140‑1 (MQ=255)                                                                                    tGAAGTTCCCAAACAGCCCGGCGGTCGCCACTGCCAACAAAATCAACCAGCGTGTCTTACGAAACACGCGTAATGGCGGCAACCTCTTCTTCACCGCAAGAATGGCACCCAGGCCAATACTCGCCATCAAGAAACGGTaa                                                    >  1:197805/1‑140 (MQ=255)                                                                                        gTTCCCAAACAGCCCGGCGGTCGCCACTGCCAACAAAATCAACCAGCGTGTCTTACGAAACACGCGTAATGGCGGCAACCTCTTCTTCACCGCAAGAATGGCACCCAGGCCAATACTCGCCATCAAGAAACGGTAAAaca                                                <  2:1203040/140‑1 (MQ=255)                                                                                        gTTCCCAAACAGCCCGGCGGTCGCCACTGCCAACAAAATCAACCAGCGTGTCTTACGAAACACGCGTAATGGCGGCAACCTCTTCTTCACCGCAAGAATGGCACCCAGGCCAATACTCGCCATCAAGAAACGGTAAAaca                                                <  1:344809/140‑1 (MQ=255)                                                                                          tCCCAAACCGCCCGGCGGTCGCCACTGCCAACAAAATCAACCAGGCTGTCTTACGAAACACGCGGAATGGCGTCCACCGCTTATTCACCGCAAGAATGGCTCTCAGGGCAGTCCTCGCCATCAATAACCGCTAAAacacc                                              <  2:1353697/140‑2 (MQ=255)                                                                                            ccAAACAGCCCGGCGGTCGCCACTGCCAACAAAATCAACCAGCGTGTCTTACGAAACACGCGTAATGGCGGCAACCTCTTCTTCACCGCAAGAATGGCACCCAGGCCAATACTCGCCATCAAGAAACGGTAAAACACGAt                                            >  1:1411844/1‑140 (MQ=255)                                                                                            ccAAACAGCCCGGCGGTCGCCACTGCCAACAAAATCAACCAGCGTGTCTTACGAAACACGCGTAATGGCGGCAACCTCTTCTTCACCGCAAGAATGGCACCCAGGCCAATACTCGCCATCAAGAAACGGTAAAACACGAt                                            <  1:1484161/140‑1 (MQ=255)                                                                                            ccAAACAGCCCGGCGGTCGCCACTGCCAACAAAATCAACCAGCGTGTCTTACGAAACACGCGTAATGGCGGCAACCTCTTCTTCACCGCAAGAATGGCACCCAGGCCAATACTCGCCATCAAGAAACGGTAAAACACGAt                                            >  2:341345/1‑140 (MQ=255)                                                                                                aCAGCCCGGCGGTCGCCACTGCCAACAAAATCAACCAGCGTGTCTTACGAAACACGCGTAATGGCGGCAAACTCTTCTTCAACGCAACAATAGCACCCCGGCCAATCCTCGCCATCAAGACAAGGGAAAAAACGAATggc                                        >  2:404078/1‑138 (MQ=255)                                                                                                   gCCCGGCGGTCGCCACTGCCAACAAAATCAACCAGCGTGTCTTACGAAACACGCGTAATGGCGGCATCCTCTTCTTCACCGCAAGAATGGCACCCAGGCCAATCCTCGCCATCAAGAAACGGTAAAACACGATTGTCGGa                                     <  1:582259/140‑1 (MQ=255)                                                                                                   gCCCGGCGGTCGCCACTGCCAACAAAATCAACCAGCGTGTCTTACGAAACACGCGTAATGGCGGCAACCTCTTCTTCACCGCAAGAATGGCACCCAGGCCAATACTCGCCATCAAGAAACGGTAAAACACGATTGTCGGa                                     >  1:23604/1‑140 (MQ=255)                                                                                                     ccGGCGGTCGCCACTGCCAACAAAATCAACCAGCGTGTCTTACGAAACACGCGTAATGGCGGCAACCTCTTCTTCACCGCAAGAATGGCACCCAGGCCAATACTCGCCATCAAGAAACGGTAAAACACGATTGTCGGAgg                                   <  2:261893/140‑1 (MQ=255)                                                                                                      cggcggTCGCCACTGCCAACAAAATCAACCAGCGTGTCTTACGAAACACGCGTAATGGCGGCAACCTCTTCTTCACCGCAAGAATGGCACCCAGGCCAATACTCGCCATCAAGAAACGGTAAAACTCGATTGTCGGAGGt                                  <  2:1371896/140‑1 (MQ=255)                                                                                                      cggcggTCGCCACTGCCAACAAAATCAACCAGCGTGTCTTACGAAACACGCGTAATGGCGGCAACCTCTTCTTCACCGCAAGAATGGCACCCAGGCCAATACTCGCCATCAAGAAACGGTAAAACACGATTGTCGGAGGt                                  >  1:432678/1‑140 (MQ=255)                                                                                                                cACTGCCAACAAAATCAACCAGCGTGTCTTACGAAACACGCGTAATGGCGGCAACCTCTTCTTCACCGCAAGAATGGCACCCAGGCCAATACTCGCCATCAAGAAACGGTAAAACACGATTGTCGGAGGTTCCATCACCt                        <  2:1388758/140‑1 (MQ=255)                                                                                                                   tGCCAACAAAATCAACCAGCGTGTCTTACGAAACACGCGTAATGGCGGCAACCTCTTCTTCCCCGCAAGAATGGCACCCAGGCCAATACTCGCCATCAAGAAACGGTAAAACACGATTGTCGGAGGTTCCATCACCTCCa                     <  2:1062724/140‑1 (MQ=255)                                                                                                                     ccAACAAAATCAACCAGCGTGTCTTACGAAACACGCGTAATGGCGGCAACCTCTTCTTCACCGCAAGAATGGCACCCAGGCCAATACTCGCCATCAAGAAACGGTAAAACACGATTGTCGGAGGTTCCATCACCTCCAGc                   <  1:86212/140‑1 (MQ=255)                                                                                                                      caacaaAATCAACCAGCGTGTCTTACGAAACACGCGTAATGGCGGCAACCTCTTCTTCACCGCAAGAATGGCACCCAGGCCAATACTCGCCATCAAGAAACGGTAAAACACGATTGTCGGAGGTTCCATCACCTCCAGCa                  >  2:213062/1‑140 (MQ=255)                                                                                                                       aacaaAATCAACCAGCGTGTCTTACGAAACACGCGTAATGGCGGCAACCTCTTCTTCACCGCAAGAATGGCACCCAGGCCAATACTCGCCATCAAGAAACGGTAAAACACGATTGTCGGAGGTTCCATCACCTCCAGCAc                 <  2:1411844/140‑1 (MQ=255)                                                                                                                            aaTCAACCAGCGTGTCTTACGAAACACGCGTAATGGCGGCAACCTCTTCTTCACCGCAAGAATGGCACCCAGGCCAATACTCGCCATCAAGAAACGGTAAAACACGATTGTCGGAGGTTCCATCACCTCCAGCACCTGCt            >  1:804507/1‑140 (MQ=255)                                                                                                                                   cAGCGTGTCTTACGAAACACGCGTAATGGCGGCAACCTCTTCTTCACCGCAAGAATGGCACCCAGGCCAATACTCGCCATCAAGAAACGGTAAAACACGATTGTCGGAGGTTCCATCACCTCCAGCACCTGCTTCATTGc     >  1:162078/1‑140 (MQ=255)                                                                                                                                    aGCGTGTCTTACGAAACACGCGTAATGGCGGCAACCTCTTCTTCACCGCAAGAATGGCACCCAGGCCAATACTCGCCATCAAGAAACGGTAAAACACGATTGTCGGAGGTTCCATCACCTCCAGCACCTGCTTCATTGCg    <  2:1290932/140‑1 (MQ=255)                                                                                                                                     gCGTGTCTTACGAAACACGCGTAATGGCGGCACCCTCTTCTTCACCGCAAGAATGGCACCCAGGCCAATACTCGCCATCAAGAAACGGTAAAACACGATTGTCGGAGGTTCCATCACCTCCAGCACCTGCTTCATTGCGa   <  1:509308/140‑1 (MQ=255)                                                                                                                                      cGTGTCTTACGAAACACGCGTAATGGCGGCAACCTCTTCTTCACCGCAAGAATGGCACCCAGGCCAATACTCGCCATCAAGAAACGGTAAAACACGATTGTCGGAGGTTCCATCACCTCCAGCACCTGCTTCATTGCGAt  >  1:667187/1‑140 (MQ=255)                                                                                                                                      cGTGTCTTACGAAACACGCGTAATGGCGGCAACCTCTTCTTCACCGCAAGAATGGCACCCAGGCCAATACTCGCCATCAAGAAACGGTAAAACACGATTGTCGGAGGTTCCATCACCTCCAGCACCTGCTTCATTGCGAt  <  2:1021892/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                        AACCATCATGCCAACTGGCGAGAGTTGCCCAATCACCTGCGAAGCGGTCGGACTCAGGTATTGCAAGGATGAGCTGAACAGGATGAAGTTCCCAAACAGCCCGGCGGTCGCCACTGCCAACAAAATCAACCAGCGTGGCTTACGAAACACGCGTAATGGCGGCAACCTCTTCTTCACCGCAAGAATGGCACCCAGGCCAATACTCGCCATCAAGAAACGGTAAAACACGATTGTCGGAGGTTCCATCACCTCCAGCACCTGCTTCATTGCGAT  >  NC_000913/3332394‑3332666

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑