Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,594,825	G→T	P219Q (CCG→CAG)	livM ←	branched chain amino acid/phenylalanine ABC transporter membrane subunit LivM

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,594,825	0	G	T	100.0%	245.3 / NA	69	P219Q (CCG→CAG)	livM	branched chain amino acid/phenylalanine ABC transporter membrane subunit LivM
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (39/30);  total (39/30)

AGGAAGATGACACGATCGGAGGGATCGTATTTCAGGCCAAAGAAATTACTGAACGTGTCCCAGCCGCCTTCACGAGCGGTACGGCTGAACTCGAGTCCGAAGAGTGTCGGTTTCGGGATCTGACTGATTCCGTTCGGGCCGCCGGTAATTTCGGTGTTATTGAGCAGCAATATGCGCACAATTTCGCCGAAACCGAGGGTAACGATCGCCAGATAGTCACCGCGCAAACGCAGCACCGGAAAACCGAGCAGGAAGCCCGCCGCCGCTGCC  >  NC_000913/3594690‑3594959                                                                                                                                        |                                                                                                                                       agGAAGATGACACGATCGGAGGGATCGTATTTCAGGCCAAAGAAATTACTGAACGTGTCCCAGCCGCCTTCACGAGCGGTACGGCTGAACTCGAGTCCGAAGAGTGTCGGTTTCGGGATCTGACTGATTCCGTTCTGgcc                                                                                                                                    >  2:1165361/1‑140 (MQ=255) agGAAGATGACACGATCGGAGGGATCGTATTTCAGGCCAAAGAAATTACTGAACGTGTCCCAGCCGCCTTCACGAGCGGTACGGCTGAACTCGAGTCCGAAGAGTGTCGGTTTCGGGATCTGACTGATTCCGTTCTGgcc                                                                                                                                    <  2:954031/140‑1 (MQ=255) agGAAGATGACACGATCGGAGGGATCGTATTTCAGGCCAAAGAAATTACTGAACGTGTCCCAGCCGCCTTCACGAGCGGTACGGCTGAACTCGAGTCCGAAGAGTGTCGGTTTCGGGATCTGACTGATTCCGTTCTGgcc                                                                                                                                    >  1:724473/1‑140 (MQ=255)      gATGACACGATCGGAGGGATCGTATTTCAGGCCAAAGAAATTACTGAACGTGTCCCAGCCGCCTTCACGAGCGGTACGGCTGAACTCGAGTCCGAAGAGTGTCGGTTTCGGGATCTGACTGATTCCGTTCTGGCCGCCgg                                                                                                                               >  2:330551/1‑140 (MQ=255)       aTGACACGATCGGAGGGATCGTATTTCAGGCCAAAGAAATTACTGAACGTGTCCCAGCCGCCTTCACGAGCGGTACGGCTGAACTCGAGTCCGAAGAGTGTCGGTTTCGGGATCTGACTGATTCCGTTCTGGCCGCCGGt                                                                                                                              >  2:1257900/1‑140 (MQ=255)       aTGACACGATCGGAGGGATCGTATTTCAGGCCAAAGAAATTACTGAACGTGTCCCAGCCGCCTTCACGAGCGGTACGGCTGAACTCGAGTCCGAAGAGTGTCGGTTTCGGGATCTGACTGATTCCGTTCTGGCCGCCGGt                                                                                                                              >  1:1280616/1‑140 (MQ=255)           cacGATCGGAGGGATCGTATTTCAGGCCAAAGAAATTACTGAACGTGTCCCAGCCGCCTTCACGAGCGGTACGGCTGAACTCGAGTCCGAAGAGTGTCGGTTTCGGGATCTGACTGATTCCGTTCTGGCCGCCGGTAAtt                                                                                                                          <  2:1280616/140‑1 (MQ=255)           cacGATCGGAGGGATCGTATTTCAGGCCAAAGAAATTACTGAACGTGTCCCAGCCGCCTTCACGAGCGGTACGGCTGAACTCGAGTCCGAAGAGTGTCGGTTTCGGGATCTGACTGATTCCGTTCTGGCCGCCGGTAAtt                                                                                                                          >  1:382250/1‑140 (MQ=255)            acGATCGGAGGGATCGTATTTCAGGCCAAAGAAATTACTGAACGTGTCCCAGCCGCCTTCACGAGCGGTACGGCTGAACTCGAGTCCGAAGAGTGTCGGTTTCGGGATCTGACTGATTCCGTTCTGGCCGCCGGTAAttt                                                                                                                         <  2:756009/140‑1 (MQ=255)              gATCGGAGGGATCGTATTTCAGGCCAAAGAAATTACTGAACGTGTCCCAGCCGCCTTCACGAGCGGTACGGCTGAACTCGAGTCCGAAGAGTGTCGGTTTCGGGATCTGACTGATTCCGTTCTGGCCGCCGGTAATTTCg                                                                                                                       >  1:1074325/1‑140 (MQ=255)                  ggAGGGATCGTATTTCAGGCCAAAGAAATTACTGAACGTGTCCCAGCCGCCTTCACGAGCGGTACGGCTGAACTCGAGTCCGAAGAGTGTCGGTTTCGGGATCTGACTGATTCCGTTCTGGCCGCCGGTAATTTCGgtgt                                                                                                                   <  1:280992/140‑1 (MQ=255)                  ggAGGGATCGTATTTCAGGCCAAAGAAATTACTGAACGTGTCCCAGCCGCCTTCACGAGCGGTACGGCTGAACTCGAGTCCGAAGAGTGTCGGTTTCGGGATCTGACTGATTCCGTTCTGGCCGCCGGTAATTTCGgtgt                                                                                                                   >  2:334070/1‑140 (MQ=255)                    aGGGATCGTATTTCAGGCCAAAGAAATTACTGAACGTGTCCCAGCCGCCTTCACGAGCGGTACGGCTGAACTCGAGTCCGAAGAGTGTCGGTTTCGGGATCTGACTGATTCCGTTCTGGCCGCCGGTAATTTCGGTGTTa                                                                                                                 <  2:776754/140‑1 (MQ=255)                    aGGGATCGTATTTCAGGCCAAAGAAATTACTGAACGTGTCCCAGCCGCCTTCACGAGCGGTACGGCTGAACTCGAGTCCGAAGAGTGTCGGTTTCGGGATCTGACTGATTCCGTTCTGGCCGCCGGTAATTTCGGTGTTa                                                                                                                 <  1:1257900/140‑1 (MQ=255)                    aGGGATCGTATTTCAGGCCAAAGAAATTACTGAACGTGTCCCAGCCGCCTTCACGAGCGGTACGGCTGAACTCGAGTCCGAAGAGTGTCGGTTTCGGGATCTGACTGATTCCGTTCTGGCCGCCGGTAATTTCGGTGTTa                                                                                                                 <  1:348748/140‑1 (MQ=255)                      ggATCGTATTTCAGGCCAAAGAAATTACTGAACGTGTCCCAGCCGCCTTCACGAGCGGTACGGCTGAACTCGAGTCCGAAGAGTGTCGGTTTCGGGATCTGACTGATTCCGTTCTGGCCGCCGGTAATTTCGGTGTTAtt                                                                                                               <  1:330551/140‑1 (MQ=255)                        aTCGTATTTCAGGCCAAAGAAATTACTGAACGTGTCCCAGCCGCCTTCACGAGCGGTACGGCTGAACTCGAGTCCGAAGAGTGTCGGTTTCGGGATCTGACTGATTCCGTTCTGGCCGCCGGTAATTTCGGTGTTATTGa                                                                                                             >  1:5749/1‑140 (MQ=255)                        aTCGTATTTCAGGCCAAAGAAATTACTGAACGTGTCCCAGCCGCCTTCACGAGCGGTACGGCTGAACTCGAGTCCGAAGAGTGTCGGTTTCGGGATCTGACTGATTCCGTTCTGGCCGCCGGTAATTTCGGTGTTATTGa                                                                                                             <  2:1183716/140‑1 (MQ=255)                        aTCGTATTTCAGGCCAAAGAAATTACTGAACGTGTCCCAGCCGCCTTCACGAGCGGTACGGCTGAACTCGAGTCCGAAGAGTGTCGGTTTCGGGATCTGACTGATTCCGTTCTGGCCGCCGGTAATTTCGGTGTTATTGa                                                                                                             <  2:1109866/140‑1 (MQ=255)                         tCGTATTTCAGGCCAAAGAAATTACTGAACGTGTCCCAGCCGCCTTCACGAGCGGTACGGCTGAACTCGAGTCCGAAGAGTGTCGGTTTCGGGATCTGACTGATTCCGTTCTGGCCGCCGGTAATTTCGGTGTTAtt                                                                                                               >  2:1257151/1‑137 (MQ=255)                         tCGTATTTCAGGCCAAAGAAATTACTGAACGTGTCCCAGCCGCCTTCACGAGCGGTACGGCTGAACTCGAGTCCGAAGAGTGTCGGTTTCGGGATCTGACTGATTCCGTTCTGGCCGCCGGTAATTTCGGTGTTAtt                                                                                                               <  1:1257151/137‑1 (MQ=255)                         tCGTATTTCAGGCCAAAGAAATTACTGAACGTGTCCCAGCCGCCTTCACGAGCGGTACGGCTGAACTCGAGTCCGAAGAGTGTCGGTTTCGGGATCTGACTGATTCCGTTCTGGCCGCCGGTAATTTCGGTGTTATTGag                                                                                                            <  2:5749/140‑1 (MQ=255)                          cGTATTTCAGGCCAAAGAAATTACTGAACGTGTCCCAGTCGCCTTCACGAGCGGTACGGCTGAACTCGAGTCCGAAGAGTGTCGGTTTCGGGATCTGACTGATTCCGTTCTGGCCGCCGGTAATTTCGGTGTTATTGagc                                                                                                           >  2:856747/1‑140 (MQ=255)                          cGTATTTCAGGCCAAAGAAATTACTGAACGTGTCCCAGCCGCCTTCACGAGCGGTACGGCTGAACTCGAGTCCGAAGAGTGTCGGTTTCGGGATCTGACTGATTCCGTTCTGGCCGCCGGTAATTTCGGTGTTATTGagc                                                                                                           >  2:938626/1‑140 (MQ=255)                          cGTATTTCAGGCCAAAGAAATTACTGAACGTGTCCCAGCCGCCTTCACGAGCGGTACGGCTGAACTCGAGTCCGAAGAGTGTCGGTTTCGGGATCTGACTGATTCCGTTCTGGCCGCCGGTAATTTCGGTGTTATTGagc                                                                                                           >  2:945141/1‑140 (MQ=255)                          cGTATTTCAGGCCAAAGAAATTACTGAACGTGTCCCAGCCGCCTTCACGAGCGGTACGGCTGAACTCGAGTCCGAAGAGTGTCGGTTTCGGGATCTGACTGATTCCGTTCTGGCCGCCGGTAATTTCGGTGTTATTGagc                                                                                                           >  1:1189607/1‑140 (MQ=255)                          cGTATTTCAGGCCAAAGAAATTACTGAACGTGTCCCAGCCGCCTTCACGAGCGGTACGGCTGAACTCGAGTCCGAAGAGTGTCGGTTTCGGGATCTGACTGATTCCGTTCTGGCCGCCGGTAATTTCGGTGTTATTGagc                                                                                                           >  1:657416/1‑140 (MQ=255)                          cGTATTTCAGGCCAAAGAAATTACTGAACGTGTCCCAGCCGCCTTCACGAGCGGTACGGCTGAACTCGAGTCCGAAGAGTGTCGGGTTCGGGATCTGACTGATTCCGTTCTGGCCGCCGGTAATTTCGGTGTTATTGagc                                                                                                           >  1:1401406/1‑140 (MQ=255)                            tATTTCAGGCCAAAGAAATTACTGAACGTGTCCCAGCCGCCTTCACGAGCGGTACGGCTGAACTCGAGTCCGAAGAGTGTCGGTTTCGGGATCTGACTGATTCCGTTCTGGCCGCCGGTAATTTCGGTGTTATTGagcag                                                                                                         >  1:1228116/1‑140 (MQ=255)                             aTTTCAGGCCAAAGAAATTACTGAACGTGTCCCAGCCGCCTTCACGAGCGGTACGGCTGAACTCGAGTCCGAAGAGTGTCGGTTTCGGGATCTGACTGATTCCGTTCTGGCCGCCGGTAATTTCGGTGTTATTGagcagc                                                                                                        <  1:135755/140‑1 (MQ=255)                             aTTTCAGGCCAAAGAAATTACTGAACGTGTCCCAGCCGCCTTCACGAGCGGTACGGCTGAACTCGAGTCCGAAGAGTGTCGGTTTCGGGATCTGACTGATTCCGTTCTGGCCGCCGGTAATTTCGGTGTTATTGagcagc                                                                                                        <  1:516334/140‑1 (MQ=255)                                  aGGCCAAAGAAATTACTGAACGTGTCCCAGCCGCCTTCACGAGCGGTACGGCTGAACTCGAGTCCGAAGAGTGTCGGTTTCGGGATCTGACTGATTCCGTTCTGGCCGCCGGTAATTTCGGTGTTATTGAGCAGCAatat                                                                                                   >  2:1075239/1‑140 (MQ=255)                                  aGGCCAAAGAAATTACTGAACGTGTCCCAGCCGCCTTCACGAGCGGTACGGCTGAACTCGAGTCCGAAGAGTGTCGGTTTCGGGATCTGACTGATTCCGTTCTGGCCGCCGGTAATTTCGGTGTTATTGAGCAGCAat                                                                                                     >  2:408193/1‑138 (MQ=255)                                  aGGCCAAAGAAATTACTGAACGTGTCCCAGCCGCCTTCACGAGCGGTACGGCTGAACTCGAGTCCGAAGAGTGTCGGTTTCGGGATCTGACTGATTCCGTTCTGGCCGCCGGTAATTTCGGTGTTATTGAGCAGCAat                                                                                                     <  1:408193/138‑1 (MQ=255)                                   ggCCAAAGAAATTACTGAACGTGTCCCAGCCGCCTTCACGAGCGGTACGGCTGAACTCGAGTCCGAAGAGTGTCGGTTTCGGGATCTGACTGATTCCGTTCTGGCCGCCGGTAATTTCGGTGTTATTGa                                                                                                             <  2:981795/129‑1 (MQ=255)                                   ggCCAAAGAAATTACTGAACGTGTCCCAGCCGCCTTCACGAGCGGTACGGCTGAACTCGAGTCCGAAGAGTGTCGGTTTCGGGATCTGACTGATTCCGTTCTGGCCGCCGGTAATTTCGGTGTTATTGa                                                                                                             >  1:981795/1‑129 (MQ=255)                                    gCCAAAGAAATTACTGAACGTGTCCCAGCCGCCTTCACGAGCGGTACGGCTGAACTCGAGTCCGAAGAGTGTCGGTTTCGGGATCTGACTGATTCCGTTCTGGCCGCCGGTAATTTCGGTGTTATTGAGCAGCAATATgc                                                                                                 >  2:551841/1‑140 (MQ=255)                                         aGAAATTACTGAACGTGTCCCAGCCGCCTTCACGAGCGGTACGGCTGAACTCGAGTCCGAAGAGTGTCGGTTTCGGGATCTGACTGATTCCGTTCTGGCCGCCGGTAATTTCGGTGTTATTGAGCAGCAATATGCGcaca                                                                                            >  2:867884/1‑140 (MQ=255)                                             aTTACTGAACGTGTCCCAGCCGCCTTCACGAGCGGTACGGCTGAACTCGAGTCCGAAGAGTGTCGGTTTCGGGATCTGACTGATTCCGTTCTGGCCGCCGGTAATTTCGGTGTTATTGAGCAGCAATATGCGCACAAttt                                                                                        <  2:162730/140‑1 (MQ=255)                                                 cTGAACGTGTCCCAGCCGCCTTCACGAGCGGTACGGCTGAACTCGAGTCCGAAGAGTGTCGGTTTCGGGATCTGACTGATTCCGTTCTGGCCGCCGGTAATTTCGGTGTTATTGAGCAGCAATATGCGCACAATTTCGcc                                                                                    <  1:945141/140‑1 (MQ=255)                                                      cGTGTCCCAGCCGCCTTCACGAGCGGTACGGCTGAACTCGAGTCCGAAGAGTGTCGGTTTCGGGATCTGACTGATTCCGTTCTGGCCGCCGGTAATTTCGGTGTTATTGAGCAGCAATATGCGCACAATTTCGCCGAAAc                                                                               >  1:537393/1‑140 (MQ=255)                                                       gtgtCCCAGTCGCCTTCACGAGCGGTACGGCTGAACTCGAGTCCGAAGAGTGTCGGTTTCGGGATCTGACTGATTCCGTTCTGGCCGCCGGTAATTTCGGTGTTATTGAGCAGCAATATGCGCACAATTTCGCCGAAAcc                                                                              <  1:856747/140‑1 (MQ=255)                                                       gtgtCCCAGCCGCCTTCACGAGCGGTACGGCTGAACTCGAGTCCGAAGAGTGTCGGTTTCGGGATCTGACTGATTCCGTTCTGGCCGCCGGTAATTTCGGTGTTATTGAGCAGCAATATGCGCACAATTTCGCCGAAAcc                                                                              <  1:1242378/140‑1 (MQ=255)                                                        tgtCCCAGCCGCCTTCACGAGCGGTACGGCTGAACTCGAGTCCGAAGAGTGTCGGTTTCGGGATCTGACTGATTCCGTTCTGGCCGCCGGTAATTTCGGTGTTATTGAGCAGCAATATGCGCACAATTTCGCCGAAAcc                                                                              <  2:412849/139‑1 (MQ=255)                                                        tgtCCCAGCCGCCTTCACGAGCGGTACGGCTGAACTCGAGTCCGAAGAGTGTCGGTTTCGGGATCTGACTGATTCCGTTCTGGCCGCCGGTAATTTCGGTGTTATTGAGCAGCAATATGCGCACAATTTCGCCGAAAcc                                                                              >  1:412849/1‑139 (MQ=255)                                                        tgtCCCAGCCGCCTTCACGAGCGGTACGGCTGAACTCGAGTCCGAAGAGTGTCGGTTTCGGGATCTGACTGATTCCGTTCTGGCCGCCGGTAATTTCGGTGTTATTGAGCAGCAATATGCGCACAATTTCGCCGAAACCg                                                                             >  2:519689/1‑140 (MQ=255)                                                                    cTTCACGAGCGGTACGGCTGAACTCGAGTCCGAAGAGTGTCGGTTTCGGGATCTGACTGATTCCGTTCTGGCCGCCGGTAATTTCGGTGTTATTGAGCAGCAATATGCGCACAATTTCGCCGAAACCGAGGGTAACGATc                                                                 >  1:105539/1‑140 (MQ=255)                                                                    cTTCACGAGCGGTACGGCTGAACTCGAGTCCGAAGAGTGTCGGTTTCGGGATCTGACTGATTCCGTTCTGGCCGCCGGTAATTTCGGTGTTATTGAGCAGCAATATGCGCACAATTTCGCCGAAACCGAGGGTAACGATc                                                                 >  1:196670/1‑140 (MQ=255)                                                                      tCACGAGCGGTACGGCTGAACTCGAGTCCGAAGAGTGTCGGTTTCGGGATCTGACTGATTCCGTTCTGGCCGCCGGTAATTTCGGTGTTATTGAGCAGCAATATGCGCACAATTTCGCCGAAACCGAGGGTAACGATCGc                                                               <  1:731133/140‑1 (MQ=255)                                                                      tCACGAGCCGTACGGCTGAACTCGAGTCCGAAGAGTGGCGGTTTCAGATGTTGACTGATTCCGTTTTGGCCGCCGGTAATTTCGGTGTGATTGAGCAGCAATATGCGCACAATTTCGCCGAAACCGAGGGTAACGATCGc                                                               <  2:1074325/140‑1 (MQ=255)                                                                        aCGAGCGGTACGGCTGAACTCGAGTCCGAAGAGTGTCGGTTTCGGGATCTGACTGATTCCGTTCTGGCCGCCGGTAATTTCGGTGTTATTGAGCAGCAATATGCGCACAATTTCGCCGAAACCGAGGGTAACGATCGCCa                                                             <  1:1075239/140‑1 (MQ=255)                                                                          gAGCGGTACGGCTGAACTCGAGTCCGAAGAGTGTCGGTTTCGGGATCTGACTGATTCCGTTCTGGCCGCCGGTAATTTCGGTGTTATTGAGCAGCAATATGCGCACAATTTCGCCGAAACCGAGGGTAACGATCGCCAGa                                                           >  1:91850/1‑140 (MQ=255)                                                                               gTACGGCTGAACTCGAGTCCGAAGAGTGTCGGTTTCGGGATCTGACTGATTCCGTTCTGGCCGCCGGTAATTTCGGTGTTATTGAGCAGCAATATGCGCACAATTTCGCCGAAACCGAGGGTAACGATCGCCAGATAGTc                                                      >  1:645856/1‑140 (MQ=255)                                                                                tACGGCTGAACTCGAGTCCGAAGAGTGTCGGTTTCGGGATCTGACTGATTCCGTTCTGGCCGCCGGTAATTTCGGTGTTATTGAGCAGCAATATGCGCACAATTTCGCCGAAACCGAGGGTAACGATCGCCAGATAGTCa                                                     >  1:818307/1‑140 (MQ=255)                                                                                           tCGAGTCCGAAGAGTGTCGGTTTCGGGATCTGACTGATTCCGTTCTGGCCGCCGGTAATTTCGGTGTTATTGAGCAGCAATATGCGCACAATTTCGCCGAAACCGAGGGTAACGATCGCCAGATAGTCACCGCGCAAACg                                          <  1:867884/140‑1 (MQ=255)                                                                                              aGTCCGAAGAGTGTCGGTTTCGGGATCTGACTGATTCCGTTCTGGCCGCCGGTAATTTCGGTGTTATTGAGCAGCAATATGCGCACAATTTCGCCGAAACCGAGGGTAACGATCGCCAGATAGTCACCGCGCAAACgcag                                       <  1:938626/140‑1 (MQ=255)                                                                                              aGTCCGAAGAGTGTCGGTTTCGGGATCTGACTGATTCCGTTCTGGCCGCCGGTAATTTCGGTGTTATTGAGCAGCAATATGCGCACAATTTCGCCGAAACCGAGGGTAACGATCGCCAGATAGTCACCGCGCAAACgcag                                       <  2:1165788/140‑1 (MQ=255)                                                                                                         tgtCGGTTTCGGGATCTGACTGATTCCGTTCTGGCCGCCGGTAATTTCGGTGTTATTGAGCAGCAATATGCGCACAATTTCGCCGAAACCGAGGGTAACGATCGCCAGATAGTCACCGCGCAAACGCAGCACCGGAAAAc                            >  2:48452/1‑140 (MQ=255)                                                                                                         tgtCGGTTTCGGGATCTGACTGATTCCGTTCTGGCCGCCGGTAATTTCGGTGTTATTGAGCAGCAATATGCGCACAATTTCGCCGAAACCGAGGGTAACGATCGCCAGATAGTCACCGCGCAAACGCAGCACCGGAAAAc                            >  2:241840/1‑140 (MQ=255)                                                                                                         tgtCGGTTTCGGGATCTGACTGATTCCGTTCTGGCCGCCGGTAATTTCGGTGTTATTGAGCAGCAATATGCGCACAATTTCGCCGAAACCGAGGGTAACGATCGCCAGATAGTCACCGCGCAAACGCAGCACCGGAAAAc                            >  2:1015329/1‑140 (MQ=255)                                                                                                         tgtCGGTTTCGGGATCTGACTGATTCCGTTCTGGCCGCCGGTAATTTCGGTGTTATTGAGCAGCAATATGCGCACAATTTCGCCGAAACCGAGGGTAACGATCGCCAGATAGTCACCGCGCAAACGCAGCACCGGAAAAc                            >  1:1346753/1‑140 (MQ=255)                                                                                                         tgtCGGTTTCGGGATCTGACTGATTCCGTTCTGGCCGCCGGTAATTTCGGTGTTATTGAGCAGCAATATGCGCACAATTTCGCCGAAACCGAGGGTAACGATCGCCAGATAGTCACCGCGCAAACGCAGCACCGGAAAAc                            >  1:725934/1‑140 (MQ=255)                                                                                                           tCGGTTTCGGGATCTGACTGATTCCGTTCTGGCCGCCGGTAATTTCGGTGTTATTGAGCAGCAATATGCGCACAATTTCGCCGAAACCGAGGGTAACGATCGCCAGATAGTCACCGCGCAAACGCAGCACCGGAAAACCg                          <  2:724473/140‑1 (MQ=255)                                                                                                           tCGGTTTCGGGATCTGACTGATTCCGTTCTGGCCGCCGGCAATTTCGGTGTTATTGAGCAGCAATATGCGCACAATTTCGCCGAAACCGAGGGTAACGATCGCCAGATAGTCACCGCGCAAACGCAGCACCGGAAAACCg                          >  1:367344/1‑140 (MQ=255)                                                                                                                    ggATCTGACTGATTCCGTTCTGGCCGCCGGTAATTTCGGTGTTATTGAGCAGCAATATGCGCACAATTTCGCCGAAACCGAGGGTAACGATCGCCAGATAGTCACCGCGCAAACGCAGCACCGGAAAACCGAGCAGGAAg                 <  1:334070/140‑1 (MQ=255)                                                                                                                           actgaTTCCGTTCTGGCCGCCGGTAATTTCGGTGTTATTGAGCAGCAATATGCGCACAATTTCGCCGAAACCGAGGGTAACGATCGCCAGATAGTCACCGCGCAAACGCAGCACCGGAAAACCGAGCAGGAAGCccgccg          >  1:771538/1‑140 (MQ=255)                                                                                                                           actgaTTCCGTTCTGGCCGCCGGTAATTTCGGTGTTATTGAGCAGCAATATGCGCACAATTTCGCCGAAACCGAGGGTAACGATCGCCAGATAGTCACCGCGCAAACGCAGCACCGGAAAACCGAGCAGGAAGCccgccg          >  1:30282/1‑140 (MQ=255)                                                                                                                              gaTTCCGTTCTGGCCGCCGGTAATTTCGGTGTTATTGAGCAGCAATATGCGCACAATTTCGCCGAAACCGAGGGTAACGATCGCCAGATAGTCACCGCGCAAACGCAGCACCGGAAAACCGAGCAGGAAGCccgccgccg       <  1:519689/140‑1 (MQ=255)                                                                                                                                ttCCGTTCTGGCCGCCGGTAATTTCGGTGTTATTGAGCAGCAATATGCGCACAATTTCGCCGAAACCGAGGGTAACGATCGCCAGATAGTCACCGCGCAAACGCAGCACCGGAAAACCGAGCAGGAAGCCCGCCGCCGCt     <  1:1076924/140‑1 (MQ=255)                                                                                                                                  ccGTTCTGGCCGCCGGTAATTTCGGTGTTATTGAGCAGCAATATGCGCACAATTTCGCCGAAACCGAGGGTAACGATCGCCAGATAGTCACCGCGCAAACGCAGCACCGGAAAACCGAGCAGGAAGCCCGCCGCCGCTGc   >  1:320999/1‑140 (MQ=255)                                                                                                                                   cGTTCTGGCCGCCGGTAATTTCGGTGTTATTGAGCAGCAATATGCGCACAATTTCGCCGAAACCGAGGGTAACGATCGCCAGATAGTCACCGCGCAAACGCAGCACCGGAAAACCGAGCAGGAAGCCCGCCGCCGCTGcc  <  2:91850/140‑1 (MQ=255)                                                                                                                                        |                                                                                                                                       AGGAAGATGACACGATCGGAGGGATCGTATTTCAGGCCAAAGAAATTACTGAACGTGTCCCAGCCGCCTTCACGAGCGGTACGGCTGAACTCGAGTCCGAAGAGTGTCGGTTTCGGGATCTGACTGATTCCGTTCGGGCCGCCGGTAATTTCGGTGTTATTGAGCAGCAATATGCGCACAATTTCGCCGAAACCGAGGGTAACGATCGCCAGATAGTCACCGCGCAAACGCAGCACCGGAAAACCGAGCAGGAAGCCCGCCGCCGCTGCC  >  NC_000913/3594690‑3594959

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑