Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,931,855	G→T	L180L (CTG→CTT)	kup →	K(+):H(+) symporter Kup

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,931,855	0	G	T	100.0%	317.8 / NA	92	L180L (CTG→CTT)	kup	K(+):H(+) symporter Kup
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (45/47);  total (45/47)

GTCGCCCCGCAGCTGGATACCTGGATAGTTCCCCTCTCAATTATCGTTCTCACTTTATTATTTATGATTCAAAAACATGGCACGGCTATGGTCGGTAAGCTGTTTGCGCCGATCATGCTGACCTGGTTTTTGATTCTGGCAGGACTGGGGTTACGTAGCATTATTGCTAACCCGGAAGTGCTGCATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGTGTTGTCG  >  NC_000913/3931718‑3931987                                                                                                                                          |                                                                                                                                     gtcgCCCCGCAGCTGGATACCTGGATAGTTCCCCTCTCAATTATCGTTCTCACTTTATTATTTATGATTCAAAAACATGGCACGGCTATGGTCGGTAAGCTGTTTGCGCCGATCATGCTGACCTGGTTTTTGATTCTTGc                                                                                                                                    >  2:1048020/1‑140 (MQ=255)   cgCCCCGCAGCTGGATACCTGGATAGTTCCCCTCTCAATTATCGTTCTCACTTTATTATTTATGATTCAAAAACATGGCACGGCTATGGTCGGTAAGCTGTTTGCGCCGATCATGCTGACCTGGTTTTTGATTCTTGCAg                                                                                                                                  >  1:1290184/1‑140 (MQ=255)    gCCCCGCAGCTGGATACCTGGATAGTTCCCCTCTCAATTATCGTTCTCACTTTATTATTTATGATTCAAAAACATGGCACGGCTATGGTCGGTAAGCTGTTTGCGCCGATCATGCTGACCTGGTTTTTGATTCTTGCAgg                                                                                                                                 <  2:651750/140‑1 (MQ=255)      cccGCAGCTGGATACCTGGATAGTTCCCCTCTCAATTATCGTTCTCACTTTATTATTTATGATTCAAAAACATGGCACGGCTATGGTCGGTAAGCTGTTTGCGCCGATCATGCTGACCTGGTTTTTGATTCTTGCAGGAc                                                                                                                               <  2:356227/140‑1 (MQ=255)      cccGCAGCTGGATACCTGGATAGTTCCCCTCTCAATTATCGTTCTCACTTTATTATTTATGATTCAAAAACATGGCACGGCTATGGTCGGTAAGCTGTTTGCGCCGATCATGCTGACCTGGTTTTTGATTCTTGCAGGAc                                                                                                                               <  1:134089/140‑1 (MQ=255)         gCAGCTGGATACCTGGATAGTTCCCCTCTCAATTATCGTTCTCACTTTATTATTTATGATTCAAAAACATGGCACGGCTATGGTCGGTAAGCTGTTTGCGCCGATCATGCTGACCTGGTTTTTGATTCTTGCAGGACTgg                                                                                                                            <  2:850668/140‑1 (MQ=255)           aGCTGGATACCTGGATAGTTCCCCTCTCAATTATCGTTCTCACTTTATTATTTATGATTCAAAAACATGGCACGGCTATGGTCGGTAAGCTGTTTGCGCCGATCATGCTGACCTGGTTTTTGATTCTTGCAGGACTgggg                                                                                                                          <  2:1204720/140‑1 (MQ=255)              tGGATACCTGGATAGTTCCCCTCTCAATTATCGTTCTCACTTTATTATTTATGATTCAAAAACATGGCACGGCTATGGTCGGTAAGCTGTTTGCGCCGATCATGCTGACCTGGTTTTTGATTCTTGCAGGACTGGGGTTa                                                                                                                       <  1:136870/140‑1 (MQ=255)              tGGATACCTAGATAGTTCCCCTCTCAATTATCGTTCTCACTTTAGTAATTAGGATTCAAAAACATGGGACTGCTATGTTCGGTTAGCTGTTTGCGCCGATCATGCTGACCTGGTTTTTGATTCTTGCAGGACTGGGGTTa                                                                                                                       <  2:1180098/140‑1 (MQ=255)               ggATACCTGGATAGTTCCCCTCTCAATTATCGTTCTCACTTTATTATTTATGATTCAAAAACATGGCACGGCTATGGTCGGTAAGCTGTTTGCGCCGATCATGCTGACCTGGTTTTTGATTCTTGCAGGACTGGGGTTAc                                                                                                                      >  1:210616/1‑140 (MQ=255)                gATACCTGGATAGTTCCCCTCTCAATTATCGTTCTCACTTTATTATTTATGATTCAAAAACATGGCACGGCTATGGTCGGTAAGCTGTTTGCACCGATCATGCTGACCTGGTTTTTGATTCTTGCAGGACTGGGGTTACg                                                                                                                     >  2:1092027/1‑140 (MQ=255)                     cTGGATAGTTCCCCTCTCAATTATCGTTCTCACTTTATTATTTATGATTCAAAAACATGGCACGGCTATGGTCGGTAAGCTGTTTGCGCCGATCATGCTGACCTGGTTTTTGATTCTTGCAGGACTGGGGTTACGTAGCa                                                                                                                <  2:1199365/140‑1 (MQ=255)                      tGGATAGTTCCCCTCTCAATTATCGTTCTCACTTTATTATTTATGATTCAAAAACATGGCACGGCTATGGTCGGTAAGCTGTTTGCGCCGATCATGCTGACCTGGTTTTTGATTCTTGCAGGACTGGGGTTACGTAGCat                                                                                                               <  1:178384/140‑1 (MQ=255)                       ggATAGTTCCCCTCTCAATTATCGTTCTCACTTTATTATTTATGATTCAAAAACATGGCACGGCTATGGTCGGTAAGCTGTTTGCGCCGATCATGCTGACCTGGTTTTTGATTCTTGCAGGACTGGGGTTACGTAGCatt                                                                                                              >  2:390117/1‑140 (MQ=255)                          tAGTTCCCCTCTCAATTATCGTTCTCTCTTTATTATTTATGATTCAAAAACATGGCACGGCTATGGTCGGTAAGCTGTTTGCGCCGATCATGCTGACCTGGTTTTTGATTCTTGCAGGACTGGGGTTACGTAGCattatt                                                                                                           <  2:349946/140‑1 (MQ=255)                          tAGTTCCCCTCTCAATTATCGTTCTCACTTTATTATTTATGATTCAAAAACATGGCACGGCTATGGTCGGTAAGCTGTTTGCGCCGATCATGCTGACCTGGTTTTTGATTCTTGCAGGACTGGGGTTACGTAGCattatt                                                                                                           >  1:1238520/1‑140 (MQ=255)                            gTTCCCCTCTCAATTATCGTTCTCACTTTATTATTTATGATTCAAAAACATGGCACGGCTATGGTCGGTAAGCTGTTTGCGCCGATCATGCTGACCTGGTTTTTGATTCTTGTAGGACTGGGGTTACGTAGCATTATTGc                                                                                                         <  2:1488031/140‑1 (MQ=255)                            gTTCCCCTCTCAATTATCGTTCTCACTTTATTATTTATGATTCAAAAACATGGCACGGCTATGGTCGGTAAGCTGTTTGCGCCGATCATGCTGACCTGGTTTTTGATTCTTGCAGGACTGGGGTTACGTAGCATTATTGc                                                                                                         <  2:1290184/140‑1 (MQ=255)                            gTTCCCCTCTCAATTATCGTTCTCACTTTATTATTTATGATTCAAAAACATGGCACGGCTATGGTCGGTAAGCTGTTTGCCCCGATCATGCTGACCTGGTTTTTGATTCTTGCAGGACTGGGGTTACGTAGCATTATTGc                                                                                                         >  2:417007/1‑140 (MQ=255)                             ttCCCCTCTCAATTATCGTTCTCACTTTATTATTTATGATTCAAAAACATGGCACGGCTATGGTCGGTAAGCTGTTTGCGCCGATCATGCTGACCTGGTTTTTGATTCTTGCAGGACTGGGGTTACGTAGCATTATTGCt                                                                                                        >  2:1489512/1‑140 (MQ=255)                             ttCCCCTCTCAATTATCGTTCTCACTTTATTATTTATGATTCAAAAACATGGCACGGCTATGGTCGGTAAGCTGTTTGCGCCGATCATGCTGACCTGGTTTTTGATTCTTGCAGGACTGGGGTTACGTAGCATTATTGCt                                                                                                        >  2:408679/1‑140 (MQ=255)                             ttCCCCTCTCAATTATCGTTCTCACTTTATTATTTATGATTCAAAAACATGGCACGGCTATGGTCGGTAAGCTGTTTGCGCCGATCATGCTGACCTGGTTTTTGATTCTTGCAGGACTGGGGTTACGTAGCATTATTGCt                                                                                                        >  1:557986/1‑140 (MQ=255)                              tCCCCTCTCAATTATCGTTCTCGCTTTATTATTTATGATTCAAAAACATGGCACGGCTATGGTCGGTAAGCTGTTTGCGCCGATCATGCTGACCTGGTTTTTGATTCTTGCAGGACTGGGGTTACGTAGCATTATTGCTa                                                                                                       <  1:222965/140‑1 (MQ=255)                                  ctctCAATTATCGTTCTCACTTTATTATTTATGATTCAAAAACATGGCACGGCTATGGTCGGTAAGCTGTTTGCGCCGATCATGCTGACCTGGTTTTTGATTCTTGCAGGACTGGGGTTACGTAGCATTATTGCTAAccc                                                                                                   >  2:584566/1‑140 (MQ=255)                                    ctcAATTATCGTTCTCACTTTATTATTTATGATTCAAAAACATGGCACGGCTATGGTCGGTAAGCTGTTTGCGCCGATCATGCTGACCTGGTTTTTGATTCTTGCAGGACTGGGGTTACGTAGCATTATTGCTAACCCgg                                                                                                 <  2:99831/140‑1 (MQ=255)                                      cAATTATCGTTCTCACTTTATTATTTATGATTCAAAAACATGGCACGGCTATGGTCGGTAAGCTGTTTGCGCCGATCATGCTGACCTGGTTTTTGATTCTTGCAGGACTGGGGTTACGTAGCATTATTGCTAACCCGGaa                                                                                               <  1:1434922/140‑1 (MQ=255)                                       aaTTATCGTTCTCACTTTATTATTTATGATTCAAAAACATGGCACGGCTATGGTCGGTAAGCTGTTTGCGCCGATCATGCTGACCTGGTTTTTGATTCTTGCAGGACTGGGGTTACGTAGCATTATTGCTAACCCGGAAg                                                                                              >  1:534684/1‑140 (MQ=255)                                        aTTATCGTTCTCACTTTATTATTTATGATTCAAAAACATGGCACGGCTATGGTCGGTAAGCTGTTTGCGCCGATCATGCTGACCTGGTTTTTGATTCTTGCAGGACTGGGGTTACGTAGCATTATTGCTAACCCGGAAGt                                                                                             <  1:1489512/140‑1 (MQ=255)                                                 ctcACTTTATTATTTATGATTCAAAAACATGGCACGGCTATGGTCGGTAAGCTGTTTGCGCCGATCATGCTGACCTGGTTTTTGATTCTTGCAGGACTGGGGTTACGTAGCATTATTGCTAACCCGGAAGTGCTGCATgc                                                                                    >  1:200618/1‑140 (MQ=255)                                                 ctcACTTTATTATTTATGATTCAAAAACATGGCACGGCTATGGTCGGTAAGCTGTTTGCGCCGATCATGCTGACCTGGTTTTTGATTCTTGCAGGACTGGGGTTACGTAGCATTATTGCTAACCCGGAAGTGCTGCATgc                                                                                    >  2:260851/1‑140 (MQ=255)                                                   cACTTTATTATTTATGATTCAAAAACATGGCACGGCTATGGTCGGTAAGCTGTTTGCGCCGATCATGCTGACCTGGTTTTTGATTCTTGCAGGACTGGGGTTACGTAGCATTATTGCTAACCCGGAAGTGTTGCATgcgc                                                                                  >  2:1365605/1‑140 (MQ=255)                                                   cACTTTATTATTTATGATTCAAAAACATGGCACGGCTATGGTCGGTAAGCTGTTTGCGCCGATCATGCTGACCTGGTTTTTGATTCTTGCAGGACTGGGGTTACGTAGCATTATTGCTAACCCGGAAGTGCTGCATgcgc                                                                                  <  1:51602/140‑1 (MQ=255)                                                   cACTTTATTATTTATGATTCAAAAACATGGCACGGCTATGGTCGGTAAGCTGTTTGCGCCGATCATGCTGACCTGGTTTTTGATTCTTGCAGGACTGGGGTTACGTAGCATTATTGCTAACCCGGAAGTGCTGCATgcgc                                                                                  <  2:534684/140‑1 (MQ=255)                                                   cACTTTATTATTTATGATTCAAAAACATGGCACGGCTATGGTCGGTAAGCTGTTTGCGCCGATCATGCTGACCTGGTTTTTGATTCTTGCAGGACTGGGGTTACGTAGCATTATTGCTAACCCGGAAGTGCTGCATgcgc                                                                                  <  1:584566/140‑1 (MQ=255)                                                   cACTTTATTATTTATGATTCAAAAACATGGCACGGCTATGGTCGGTAAGCTGTTTGCGCCGATCATGCTGACCTGGTTTTTGATTCTTGCAGGACTGGGGTTACGTAGCATTATTGCTAACCCGGAAGTGCTGCATgcgc                                                                                  <  1:584953/140‑1 (MQ=255)                                                   cACTTTATTATTTATGATTCAAAAACATGGCACGGCTATGGTCGGTAAGCTGTTTGCGCCGATCATGCTGACCTGGTTTTTGATTCTTGCAGGACTGGGGTTACGTAGCATTATTGCTAACCCGGAAGTGCTGCATgcgc                                                                                  >  1:615002/1‑140 (MQ=255)                                                    aCTTTATTATTTATGATTCAAAAACATGGCACGGCTATGGTCGGTAAGCTGTTTGCGCCGATCATGCTGACCTGGTTTTTGATTCTTGCAGGACTGGGGTTACGTAGCATTATTGCTAACCCGGAAGTGCTGCATGCGCt                                                                                 <  2:210616/140‑1 (MQ=255)                                                    aCTTTATTATTTATGATTCAAAAACATGGCACGGCTATGGTCGGTAAGCTGTTTGCGCCGATCATGCTGACCTGGTTTTTGATTCTTGCAGGACTGGGGTTACGTAGCATTATTGCTAACCCGGAAGTGCTGCATGCGCt                                                                                 <  1:1248560/140‑1 (MQ=255)                                                       ttattaTTTATGATTCAAAAACATGGCACGGCTATGGTCGGTAAGCTGTTTGCGCCGATCATGCTGACCTGGTTTTTGATTCTTGCAGGACTGGGGTTACGTAGCATTATTGCTAACCCGGAAGTGCTGCATGCGCTGaa                                                                              <  1:656999/140‑1 (MQ=255)                                                        tattatTTATGATTCAAAAACATGGCACGGCTATGGTCGGTAAGCTGTTTGCGCCGATCATGCTGACCTGGTTTTTGATTCTTGCAGGACTGGGGTTACGTAGCATTATTGCTAACCCGGAAGTGCTGCATGCGCTGAAt                                                                             <  2:557986/140‑1 (MQ=255)                                                         attattTATGATTCAAAAACATGGCACGGCTATGGTCGGTAAGCTGTTTGCGCCGATCATGCTGACCTGGTTTTTGATTCTTGCAGGACTGGGGTTACGTAGCATTATTGCTAACCCGGAAGTGCTGCATGCGCTGAATc                                                                            >  2:49018/1‑140 (MQ=255)                                                         attattTATGATTCAAAAACATGGCACGGCTATGGTCGGTAAGCTGTTTGCGCCGATCATGCTGACCTGGTTTTTGATTCTTGCAGGACTGGGGTTACGTAGCATTATTGCTAACCCGGAAGTGCTGCATGCGCTGAATc                                                                            >  1:448224/1‑140 (MQ=255)                                                          ttatttatGATTCAAAAACATGGCACGGCTATGGTCGGTAAGCTGTTTGCGCCGATCATGCTGACCTGGTTTTTGATTCTTGCAGGACTGGGGTTACGTAGCATTATTGCTAACCCGGAAGTGCTGCATGCGCTGAATcc                                                                           >  2:1301296/1‑140 (MQ=255)                                                              ttgtGATTCAAAAACATGGCACGGCTATGGTCGGTAAGCTGTTTGCGCCGATCATGCTGACCTGGTTTTTGATTCTTGCAGGACTGGGGTTACGTAGCATTATTGCTAACCCGGAAGTGCTGCATGCGCTGAATCCAAtg                                                                       >  2:1419695/4‑140 (MQ=255)                                                              ttatGATTCAAAAACATGGCACGGCTATGGTCGGTAAGCTGTTTGCGCCGATCATGCTGACCTGGTTTTTGATTCTTGCAGGACTGGGGTTACGTAGCATTATTGCTAACCCGGAAGTGCTGCATGCGCTGAATCCAAtg                                                                       >  2:418602/1‑140 (MQ=255)                                                                  gATTCAAAAACATGGCACGGCTATGGTCGGTAAGCTGTTTGCGCCGATCATGCTGACCTGGTTTTTGATTCTTGCAGGACTGGGGTTACGTAGCATTATTGCTAACCCGGAAGTGCTGCATGCGCTGAATCCAATGTggg                                                                   <  1:49018/140‑1 (MQ=255)                                                                  gATTCAAAAACATGGCACGGCTATGGTCGGTAAGCTGTTTGCGCCGATCATGCTGACCTGGTTTTTGATTCTTGCAGGACTGGGGTTACGTAGCATTATTGCTAACCCGGAAGTGCTGCATGCGCTGAATCCAATGTggg                                                                   <  2:1238520/140‑1 (MQ=255)                                                                   aTTCAAAAACATGGCACGGCTATGGTCGGTAAGCTGTTTGCGCCGATCATGCTGACCTGGTTTTTGATTCTTGCAGGACTGGGGTTACGTAGCATTATTGCTAACCCGGAAGTGCTGCATGCGCTGAATCCAATGTGGGc                                                                  >  1:1248994/1‑140 (MQ=255)                                                                    ttCAAAAACATGGCACGGCTATGGTCGGTAAGCTGTTTGCGCCGATCATGCTGACCTGGTTTTTGATTCTTGCAGGACTGGGGTTACGTAGCATTATTGCTAACCCGGAAGTGCTGCATGCGCTGAATCCAATGTGGGCg                                                                 <  2:1318539/140‑1 (MQ=255)                                                                    ttCAAAAACATGGCACGGCTATGGTCGGTAAGCTGTTTGCGCCGATCATGCTGACCTGGTTTTTGATTCTTGCAGGACTGGGGTTACGTAGCATTATTGCTAACCCGGAAGTGCTGCATGCGCTGAATCCAATGTGGGCg                                                                 >  2:1149623/1‑140 (MQ=255)                                                                    ttCAAAAACATGGCACGGCTATGGTCGGTAAGCTGTTTGCGCCGATCATGCTGACCTGGTTTTTGATTCTTGCAGGACTGGGGTTACGTAGCATTATTGCTAACCCGGAAGTGCTGCATGCGCTGAATCCAATGTGGGCg                                                                 >  1:1140388/1‑140 (MQ=255)                                                                    ttCAAAAACATGGCACGGCTATGGTCGGTAAGCTGTTTGCGCCGATCATGCTGACCTGGTTTTTGATTCTTGCAGGACTGGGGTTACGTAGCATTATTGCTAACCCGGAAGTGCTGCATGCGCTGAATCCAATGTGGGCg                                                                 >  2:1111202/1‑140 (MQ=255)                                                                    ttCAAAAACATGGCACGGCTATGGTCGGTAAGCTGTTTGCGCCGATCATGCTGACCTGGTTTTTGATTCTTGCAGGACTGGGGTTACGTAGCATTATTGCTAACCCGGAAGTGCTGCATGCGCTGAATCCAATGTGGGCg                                                                 >  1:121049/1‑140 (MQ=255)                                                                    ttCAAAAACATGGCACGGCTATGGTCGGTAAGCTGTTTGCGCCGATCATGCTGACCTGGTTTTTGATTCTTGCAGGACTGGGGTTACGTAGCATTATTGCTAACCCGGAAGTGCTGCATGCGCTGAATCCAATGTGGGCg                                                                 >  1:1344189/1‑140 (MQ=255)                                                                    ttCAAAAACATGGCACGGCTATGGTCGGTAAGCTGTTTGCGCCGATCATGCTGACCTGGTTTTTGATTCTTGCAGGACTGGGGTTACGTAGCATTATTGCTAACCCGGAAGTGCTGCATGCGCTGAATCCAATGTGGGCg                                                                 >  1:777792/1‑140 (MQ=255)                                                                    ttCAAAAACATGGCACGGCTATGGTCGGTAAGCTGTTTATGCAGATCATACTGACCTGGTTTTTGATTCTTGCAGGACTGGGGTTACGTAGCATTATTGCTAACCCGGAAGAGCTGCATGCGATGAATCCAATGTGGACg                                                                 >  2:1146073/1‑140 (MQ=255)                                                                     tCAAAAACATGGCACGGCTATGGTCGGTAAGCTGTTTGCGCCGATCATGCTGACCTGGTTTTTGATTCTTGCAGGACTGGGGTTACGTAGCATTATTGCTAACCCGGAAGTGCTGCATGCGCTGAATCCAATGTGGGCgg                                                                <  1:891944/140‑1 (MQ=255)                                                                       aaaaaCATGGCACGGCTATGGTCGGTAAGCTGTTTGCGCCGATCATGCTGACCTGGTTTTTGATTCTTGCAGGACTGGGGTTACGTAGCATTATTGCTAACCCGGAAGTGCTGCATGCGCTGAATCCAATGTGGGCGGTg                                                              <  2:200618/140‑1 (MQ=255)                                                                       aaaaaCATGGCAAGGGTCAGTCGGGTAAGCTGTTTGCGCCGATCATGCGGCAATAAAGTTTGCTTCCTGCAGGACTGGGGTTACGAAGCATTATTTATAACCCGGAAGTGTTGCATGCGCTGAATCCAATGTGGGCGGTg                                                              >  2:1064323/1‑140 (MQ=255)                                                                              tGGCACGGCTATGGTCGGTAAGCTGTTTGCGCCGATCATGCTGACCTGGTTTTTGATTCTTGCAGGACTGGGGTTACGTAGCATTATTGCTAACCCGGAAGTGCTGCATGCGCTGAATCCAATGTGGGCGGTGCATttct                                                       <  1:408679/140‑1 (MQ=255)                                                                              tGGCACGGCTATGGTCGGTAAGCTGTTTGCGCCGATCATGCTGACCTGGTTTTTGATTCTTGCAGGACTGGGGTTACGTAGCATTATTGCTAACCCGGAAGTGCTGCATGCGCTGAATCCAATGTGGGCGGTGCATttct                                                       <  1:1048020/140‑1 (MQ=255)                                                                              tGGCACGGCTATGGTCGGTAAGCTGTTTGCGCCGATCATGCTGACCTGGTTTTTGATTCTTGCAGGACTGGGGTTACGTAGCATTATTGCTAACCCGGAAGTGCTGCATGCGCTGAATCCAATGTGGGCGGTGCATttct                                                       >  2:759095/1‑140 (MQ=255)                                                                              tGGCACGGCTATGGTCGGTAAGCTGTTTGCGCCGATCATGCTGACCTGGTTTTTGATTCTTGCAGGACTGGGGTTACGTAGCATTATTGCTAACCCGGAAGTGCTGCATGCGCTGAATCCAATGTGGGCGGTGCATttct                                                       >  1:149102/1‑140 (MQ=255)                                                                               ggCACGGCTATGGTCGGTAAGCTGTTTGCGCCGATCATGCTGACCTGGTTTTTGATTCTTGCAGGACTGGGGTTACGTAGCATTATTGCTAACCCGGAAGTGCTGCATGCGCTGAATCCAATGTGGGCGGTGCATttctt                                                      >  1:75011/1‑140 (MQ=255)                                                                                 cACGGCTATGGTCGGTAAGCTGTTTGCGCCGATCATGCTGACCTGGTTTTTGATTCTTGCAGGACTGGGGTTACGTAGCATTATTGCTAACCCGGAAGTGCTGCATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTcc                                                    <  2:615002/140‑1 (MQ=255)                                                                                 cACGGCTATGGTCGGTAAGCTGTTTGCGCCGATCATGCTGACCTGGTTTTTGATTCTTGCAGGACTGGGGTTACGTAGCATTATTGCTAACCCGGAAGTGCTGCATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTcc                                                    <  1:842661/140‑1 (MQ=255)                                                                                 cACGGCTATGGTCGGTAAGCTGTTTGCGCCGATCATGCTGACCTGGTATTTGATTCTTGCAGGACTTGGGTTACGTAGCATTATTGCTAACCCGGAAGTGCCGCATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTcc                                                    >  2:722150/1‑140 (MQ=255)                                                                                    ggCTATGGTCGGTAAGCTGTTTGCGCCGATCATGCTGACCTGGTTTTTGATTCTTGCAGGACTGGGGTTACGTAGCATTATTGCTAACCCGGAAGTGCTGCATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTg                                                 <  2:872784/140‑1 (MQ=255)                                                                                     gCTATGGTCGGTAAGCTGTTTGCGCCGATCATGCTGACCTGGTTTTTGATTCTTGCAGGACTGGGGTTACGTAGCATTATTGCTAACCCGGAAGTGCTGCATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGa                                                >  2:1159560/1‑140 (MQ=255)                                                                                      cTATGGTCGGTAAGCTGTTTGCGCCGATCATGCTGACCTGGTTTTTGATTCTTGCAGGACTGGGGTTACGTAGCATTATTGCTAACCCGGAAGTGCTGCATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGaa                                               >  1:777986/1‑140 (MQ=255)                                                                                         tGGTCGGTAAGCTGTTTGCGCCGATCATGCTGACCTGGTTTTTGATTCTTGCAGGACTGGGGTTACGTAGCATTATTGCTAACCCGGAAGTGCTGCATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATAc                                            >  2:507429/1‑140 (MQ=255)                                                                                          ggTCGGTAAGCTGTTTGCGCCGATCATGCTGACCTGGTTTTTGATTCTTGCAGGACTGGGGTTACGTAGCATTATTGCTAACCCGGAAGTGCTGCATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACa                                           >  2:1161285/1‑140 (MQ=255)                                                                                           gTCGGTAAGCTGTTTGCGCCGATCATGCTGACCTGGTTTTTGATTCTTGCAGGACTGGGGTTACGTAGCATTATTGCTAACCCGGAAGTGCTGCATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACaa                                          <  1:1024478/140‑1 (MQ=255)                                                                                           gTCGGTAAGCTGTTTGCGCCGATCATGCTGACCTGGTTTTTGATTCTTGCAGGACTGGGGTTACGTAGCATTATTGCTAACCCGGAAGTGCTGCATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACaa                                          >  1:643258/1‑140 (MQ=255)                                                                                              ggTAAGCTGTTTGCGCCGATCATGCTGACCTGGTTTTTGATTCTTGCAGGACTGGGGTTACGTAGCATTATTGCTAACCCGGAAGTGCTGCATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAAc                                       >  1:221196/1‑140 (MQ=255)                                                                                               gTAAGCTGTTTGCGCCGATCATGCTGACCTGGTTTTTGATTCTTGCAGGACTGGGGTTACGTAGCATTATTGCTAACCCGGAAGTGCTGCATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGaa                                               <  2:615921/131‑1 (MQ=255)                                                                                               gTAAGCTGTTTGCGCCGATCATGCTGACCTGGTTTTTGATTCTTGCAGGACTGGGGTTACGTAGCATTATTGCTAACCCGGAAGTGCTGCATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGaa                                               >  1:615921/1‑131 (MQ=255)                                                                                                tAAGCTGTTTGCGCCGATCATGCTGACCTGGTTTTTGATTCTTGCAGGACTGGGGTTACGTAGCATTATTGCTAACCCGGAAGTGCTGCATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACgg                                     >  1:239724/1‑140 (MQ=255)                                                                                                 aaGCTGTTTGCACCGATCATGCTGACCTGGTTTTTGATTCTTGCAGGACTGGGGTTACGTAGCATTATTGCTAACCCGGAAGTGCTGCATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGt                                    <  1:1092027/140‑1 (MQ=255)                                                                                                        ttGCGCCGATCATGCTGACCTGGTTTTTGATTCTTGCAGGACTGGGGTTACGTAGCATTATTGCTAACCCGGAAGTGCTGCATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCtttt                             <  1:1365605/140‑1 (MQ=255)                                                                                                        ttGCGCCGATCATGCTGACCTGGTTTTTGATTCTTGCAGGACTGGGGTTACGTAGCATTATTGCTAACCCGGAAGTGCTGCATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCtttt                             <  2:121049/140‑1 (MQ=255)                                                                                                         tGCGCCGATCATGCTGACCTGGTTTTTGATTCTTGCAGGACTGGGGTTACGTAGCATTATTGCTAACCCGGAAGTGCTGCATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTa                            <  2:149102/140‑1 (MQ=255)                                                                                                          gcgcCGATCATGCTGACCTGGTTTTTGATTCTTGCAGGACTGGGGTTACGTAGCATTATTGCTAACCCGGAAGTGCTGCATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTAt                           <  2:239724/140‑1 (MQ=255)                                                                                                          gcgcCGATCATGCTGACCTGGTTTTTGATTCTTGCAGGACTGGGGTTACGTAGCATTATTGCTAACCCGGAAGTGCTGCATGCGCTGAATCCAATGTGGGAGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTAt                           >  2:1485001/1‑140 (MQ=255)                                                                                                           cgcCGATCATGCTGACCTGGTTTTTGATTCTTGCAGGACTGGGGTTACGTAGCATTATTGCTAACCCGGAAGTGCTGCATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTAtt                          <  2:777986/140‑1 (MQ=255)                                                                                                           cgcCGATCATGCTGACCTGGTTTTTGATTCTTGCAGGACTGGGGTTACGTAGCATTATTGCTAACCCGGAAGTGCTGCATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTAtt                          <  2:75011/140‑1 (MQ=255)                                                                                                                aTCATGCTGACCTGGTTTTTGATTCTTGCAGGACTGGGGTTACGTAGCATTATTGCTAACCCGGAAGTGCTGCATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCAtt                     <  1:390117/140‑1 (MQ=255)                                                                                                                 tCATGCTGACCTGGTTTTTGATTCTTGCAGGACTGGGGTTACGTAGCATTATTGCTAACCCGGAAGTGCTGCATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTa                    >  2:771979/1‑140 (MQ=255)                                                                                                                 tCATGCTGACCTGGTTTTTGATTCTTGCAGGACTGGGGTTACGTAGCATAATTGTTAACCCGGTAGGGCTGCATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGATTACAAAACGGTTTCTTTTATTGCATTa                    >  2:67002/1‑140 (MQ=255)                                                                                                                     gCTGACCTGGTTTTTGATTCTTGCAGGACTGGGGTTACGTAGCATTATTGCTAACCCGGAAGTGCTGCATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAgggg                <  2:1261144/140‑1 (MQ=255)                                                                                                                          ccTGGTTTTTGATTCTTGCAGGACTGGGGTTACGTAACATTATTGCTAACCCGGAAGTGCTGCATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAgtg           >  2:277435/1‑140 (MQ=255)                                                                                                                           cTGGTTTTTGATTCTTGCAGGACTGGGGTTACGTAGCATTATTGCTAACCCGGAAGTGCTGCATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAgtgg          >  1:506577/1‑140 (MQ=255)                                                                                                                              gTTTTTGATTCTTGCAGGACTGGGGTTACGTAGCATTATTGCTAACCCGGAAGTGCTGCATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCAtt                     >  2:575449/1‑126 (MQ=255)                                                                                                                              gTTTTTGATTCTTGCAGGACTGGGGTTACGTAGCATTATTGCTAACCCGGAAGTGCTGCATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCAtt                     <  1:575449/126‑1 (MQ=255)                                                                                                                              gTTTTTGATTCTTGCAGGACTGGGGTTACGTAGCATTATTGCTAACCCGGAAGTGCTGCATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGgtgt       >  2:1340751/1‑140 (MQ=255)                                                                                                                               ttttnNNNTCTTGCAGGACTGGGGTTACGTAGCATTATTGCTAACCCGGAAGTGCTGCATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGtgtt      <  2:448224/140‑1 (MQ=255)                                                                                                                                   tGATTCTTGCAGGACTGGGGTTACGTAGCATTATTGCTAACCCAGAAGTGCTGCATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGTGTTGTCg  >  1:1052050/1‑140 (MQ=255)                                                                                                                                          |                                                                                                                                     GTCGCCCCGCAGCTGGATACCTGGATAGTTCCCCTCTCAATTATCGTTCTCACTTTATTATTTATGATTCAAAAACATGGCACGGCTATGGTCGGTAAGCTGTTTGCGCCGATCATGCTGACCTGGTTTTTGATTCTGGCAGGACTGGGGTTACGTAGCATTATTGCTAACCCGGAAGTGCTGCATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCTTGAATACAAAACGGTTTCTTTTATTGCATTAGGGGCAGTGGTGTTGTCG  >  NC_000913/3931718‑3931987

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑