Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,111,769	G→T	S149* (TCG→TAG)	yiiQ ←	DUF1454 domain‑containing protein YiiQ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,111,769	0	G	T	100.0%	213.0 / NA	60	S149* (TCG→TAG)	yiiQ	DUF1454 domain‑containing protein YiiQ
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (31/29);  total (31/29)

CAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAGCCCCTTTT‑CGCC‑GTTGTCC‑GCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCC‑CCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAGTGTGCGGATCACCGCTGCCATGTATTCCTGAGCTTTCGCTTTCGCGGCTTTTTGCTCTGGCCCCTGGATGGGTAGCCAGGT  >  NC_000913/4111632‑4111896                                                                                                                                              |                                                                                                                                cAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAGCCCCTTTT‑CGCC‑GTTGTCC‑GCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCC‑CCGCCGTTAGTAGCTAc                                                                                                                               >  2:963148/1‑140 (MQ=255) cAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAGCCCCTTTT‑CGCC‑GTTGTCC‑GCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCC‑CCGCCGTTAGTAGCTAc                                                                                                                               >  1:1362325/1‑140 (MQ=255)                 cgcCAGCTTAATCGGTTCAACAGCGAAGGTCAGCCCCTTTT‑CGCC‑GTTGTCC‑GCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCC‑CCGCCGTTAGTAGCTACTGCAGTTTTTTCTGGc                                                                                                               >  1:554099/1‑140 (MQ=255)                  gcCAGCTTAATCGGTTCAACAGCGAAGGTCAGCCCCTTTT‑CGCC‑GTTGTCC‑GCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCC‑CCGCCGTTAGTAGCTACTGCAGTTTTTTCTGGCt                                                                                                              >  2:1294554/1‑140 (MQ=255)                  gcCAGCTTAATCGGTTCAACAGCGAAGGTCAGCCCCTTTT‑CGCC‑GTTGTCC‑GCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCC‑CCGCCGTTAGTAGCTACTGCAGTTTTTTCTGGCt                                                                                                              >  1:1395337/1‑140 (MQ=255)                  cccAGTTTAATGGTTTAAACAGCGAGGGCCCCCCCTTTTC‑CGCC‑GTTGCCC‑GCGACAACATACCGCAGTCCCCCTTCTTTCTGGGTTTAATATGTTTTTTTTTTCC‑CCGCCGTTAGTAGCTACTGCAGTTTTTTCTGGCt                                                                                                              <  2:1166744/139‑1 (MQ=255)                   ccAGCTTAATCGGTTCAACAGCGAAGGTCAGCCCCTTTT‑CGCC‑GTTGTCC‑GCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCC‑CCGCCGTTAGTAGCTACTGCAGTTTTTTCTGGCtt                                                                                                             >  1:590506/1‑140 (MQ=255)                       cTTAATCGGTTCAACAGCGAAGGTCAGCCCCTTTT‑CGCC‑GTTGTCC‑GCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCC‑CCGCCGTTAGTAGCTACTGCAGTTTTTTCTGGCTTtgtg                                                                                                         >  2:279407/1‑140 (MQ=255)                         tAATCGGTACAACAGCGAGGGTCCCCCCTTTTT‑CGCT‑TTTCTCC‑GCGACAACATAACGCAGTGCCCTTTCTGTCTGGGTTTAATATTGTTTTTTTTTCC‑CCGCCGTTAGTAGCTACTGCAGTTTTTTCTGGC‑TTGTGttt                                                                                                      <  2:821116/140‑1 (MQ=255)                           aTCGGTTCAACAGCGAAGGTCAGCCCCTTTT‑CGCC‑GTTGTCC‑GCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCC‑CCGCCGTTAGTAGCTACTGCAGTTTTTTCTGGCTTTGTGtttt                                                                                                     >  1:861589/1‑140 (MQ=255)                                ttCAACAGCGAAGGTCAGCCCCTTTT‑CGCC‑GTTGTCC‑GCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCC‑CCGCCGTTAGTAGCTACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCa                                                                                                >  1:369601/1‑140 (MQ=255)                                 tCAACAGCGAAGGTCAGCCCCTTTT‑CGCC‑GTTGTCC‑GCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCC‑CCGCCGTTAGTAGCTACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCAt                                                                                               >  1:1310404/1‑140 (MQ=255)                                        cGAAGGTCAGCCCCTTTT‑CGCC‑GTTGTCC‑GCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCC‑CCGCCGTTAGTAGCTACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATggg                                                                                        >  2:1180668/1‑140 (MQ=255)                                        cGAAGGTCAGCCCCTTTT‑CGCC‑GTTGTCC‑GCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCC‑CCGCCGTTAGTAGCTACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATggg                                                                                        >  1:870704/1‑140 (MQ=255)                                           aggccccccccTTTT‑TCCC‑GGTGGCCGGCAAAAATATACCGCGGTCCACCTTGTGTCGGGGTGTAATAGCTTTTTTTTTCCC‑CCGCCGTTAGTAGCTACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGt                                                                                      <  2:1357716/133‑1 (MQ=255)                                           aGGTCCACCCCTTTC‑CCCCGGTTGCCC‑GCAACAACATACCGCAGTCTTTTTTCTGTCTGGGTTTAATATCTTTTTTTTTCCC‑CCGCCGTTAGTAGCTACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGt                                                                                      <  2:1405367/140‑1 (MQ=255)                                             gtcccccccTTTTCCGCC‑TTTGTCC‑GCAAAAAAAAAACGCAGTCCCCTTTCTGTCTGGGTTTAATATTTTTTTTTTTCCC‑CCGCCGTTAGTAGCTACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGa                                                                                    <  2:369601/135‑1 (MQ=255)                                                       ttt‑CGCC‑GTTGTCC‑GCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCC‑CCGCCGTTAGTAGCTACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAGTGTGCGGATc                                                                         >  1:233309/1‑140 (MQ=255)                                                       ttt‑CGCC‑GTTGTCC‑GCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCC‑CCGCCGTTAGTAGCTACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAGTGTGCGGATc                                                                         >  1:108845/1‑140 (MQ=255)                                                             gctgTTGTCC‑GCGACAACATAACGCGTTGCTCCTTCTGTCTGGGTGTAATATTTTTTTTTTTCCC‑CCGCCGTTAGTAGCTACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAGTGTGCGGATCACCg                                                                     <  2:554099/137‑1 (MQ=255)                                                             ccctttGCCC‑GCGAAAAAAAAACGCATTCCCCTTTCTGTCTGGGTTTAATAATTTTTTTTTTCCC‑CCGCCGTTAGTAGCTACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAGTGTGCGGATCACCg                                                                     <  2:707849/136‑1 (MQ=255)                                                              c‑GTTTTCC‑GCAAAAACATCACGCGGTCCCCCTTCTGTCGCGGTTTAATATCTTTTTTTTTCCC‑CCGCCGTTAGTAGCTACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAGTGTGCGGATCACCGCt                                                                   <  2:310081/140‑1 (MQ=255)                                                               tgTTGCCC‑GCGACAACATACCGCAGTGCCCCTTCTGTCGGGGTTTAATAACTTTTTTTTTTCC‑CCGCCGTTAGTAGCTACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAGTGTGCGGATCACCGCt                                                                   <  2:870704/139‑1 (MQ=255)                                                                tttGCCC‑GCGACATCATACCGCTGTGCCCTTTCTTTTGGGGTGTAA‑GACTTTTTTTTTTCCACCGCCGTTAGTAGCTACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAGTGTGCGGATCACCGCTg                                                                  <  2:590506/139‑1 (MQ=255)                                                                gTTGCCC‑GCGACAACATAACGCATTCCCCCTTCTTTCTGGGTGTAATACTGTTTTTTTTCCC‑CCGCCGTTAGTAGCTACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAGTGTGCGGATCACCGCTg                                                                  <  2:154083/140‑1 (MQ=255)                                                                  tgccc‑GCGACAAAATACCGCAGTCCCCCTTCTGTCTGGGTGTAATAACTTTTTTTTTTCC‑CCGCCGTTAGTAGCTACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAGTGTGCGGATCACCGCTGcc                                                                <  1:963148/137‑1 (MQ=255)                                                                  tgccc‑GCAACAACATAACGCATTCCCCCTTCTTTCTGGGTGTAATAAGTTTTTTTTTTCC‑CCGCCGTTAGTAGCTACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAGTGTGCGGATCACCGCTGcc                                                                <  2:1323642/137‑1 (MQ=255)                                                                     cc‑GCGACAACATAACGCAGTGCACCTTCTGTCTGGGTTTATTAATTTTTTTTTTCCC‑CCGCCGTTAGTAGCTACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAGTGTGCGGATCACCGCTGCCATg                                                             <  2:168725/140‑1 (MQ=255)                                                                               caTAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCC‑CCGCCGTTAGTAGCTACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAGTGTGCGGATCACCGCTGCCATGTATTCCTGa                                                    >  2:553428/1‑140 (MQ=255)                                                                               caTAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCC‑CCGCCGTTAGTAGCTACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAGTGTGCGGATCACCGCTGCCATGTATTCCTGa                                                    >  1:540179/1‑140 (MQ=255)                                                                                         tGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCC‑CCGCCGTTAGTAGCTACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAGTGTGCGGATCACCGCTGCCATGTATTCCTGAgctttcgctt                                          >  1:1001694/1‑140 (MQ=255)                                                                                         tGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCC‑CCGCCGTTAGTAGCTACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAGTGTGCGGATCACCGCTGCCATGTATTCCTGAgctttcgctt                                          >  2:995977/1‑140 (MQ=255)                                                                                         tGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCC‑CCGCCGTTAGTAGCTACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAGTGTGCGGATCACCGCTGCCATGTATTCCTGAgctttcgctt                                          >  1:1273469/1‑140 (MQ=255)                                                                                         tGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCC‑CCGCCGTTAGTAGCTACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAGTGTGCGGATCACCGCTGCCATGTATTCCTGAgctttcgctt                                          >  1:518359/1‑140 (MQ=255)                                                                                         tGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCC‑CCGCCGTTAGTAGCTACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGGGAGTGTGCGGATCACCGCTGCCATGTATTCCTGAgctttcgctt                                          >  2:1397168/1‑140 (MQ=255)                                                                                              cTTCTGTCTCGGTGTAATACTGTTTTTTTTCCC‑CCGCCGTTAGTAGCTACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAGTGTGCGGATCACCGCTGCCATGTATTCCTGAGCTTTCGCTTTcgcg                                     <  2:233309/140‑1 (MQ=255)                                                                                              cTTCTGTCTCGGTGTAATAACGTTTGTTTTTCC‑CCGCCGTTAGTAGCTACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAGTGTGCGGATCACCGCTGCCATGTATTCCTGAGCTTTCGCTTTcgcg                                     >  1:1218076/1‑140 (MQ=255)                                                                                                   gTCTCGGTGTAATAACGTTTGTTTTTCC‑CCGCCGTTAGTAGCTACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAGTGTGCGGATCACCGCTGCCATGTATTCCTGAGCTTTCGCTTTCGCGGCttt                                >  2:1468575/1‑140 (MQ=255)                                                                                                     ctcGGTGTAATAACGTTTGTTTTTCC‑CCGCCGTTAGTAGCTACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAGTGTGCGGATCACCGCTGCCATGTATTCCTGAGCTTTCGCTTTCGCGGCttttt                              >  2:306265/1‑140 (MQ=255)                                                                                                      ggggTTTAATAATTTTTTTTTTCCC‑CCGCCGTTAGTAGCTACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAGTGTGCGGATCACCGCTGCCATGTATTCCTGAGCTTTCGCTTTCGCGGCTTTTTg                             <  2:881924/138‑1 (MQ=255)                                                                                                        ggttaaatatCTTTTTTTTTCCC‑CCGCCGTTAGTAGCTACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAGTGTGCGGATCACCGCTGCCATGTATTCCTGAGCTTTCGCTTTCGCGGCTTTTTGct                           <  2:1022691/135‑1 (MQ=255)                                                                                                          tgtAATAGTGTTTTTTTTTCC‑CCGCCGTTAGTAGCTACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAGTGTGCGGATCACCGCTGCCATGTATTCCTGAGCTTTCGCTTTCGCGGCTTTTTGctct                         <  2:843921/140‑1 (MQ=255)                                                                                                             aatattgtttttttTCCC‑CCGCCGTTAGTAGCTACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAGTGTGCGGATCACCGCTGCCATGTATTCCTGAGCTTTCGCTTTCGCGGCTTTTTGCTCTGGc                      <  2:1001694/134‑1 (MQ=255)                                                                                                              atatgtttttttttCCC‑CCGCCGTTAGTAGCTACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAGTGTGCGGATCACCGCTGCCATGTATTCCTGAGCTTTCGCTTTCGCGGCTTTTTGCTCTGGcc                     <  1:1294554/134‑1 (MQ=255)                                                                                                              ataaCGTTTGTTTTTCC‑CCGCCGTTAGTAGCTACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAGTGTGCGGATCACCGCTGCCATGTATTCCTGAGCTTTCGCTTTCGCGGCTTTTTGCTCTGGcc                     <  1:995977/140‑1 (MQ=255)                                                                                                              ataaCGTTTGTTTTTCC‑CCGCCGTTAGTAGCTACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAGTGTGCGGATCACCGCTGCCATGTATTCCTGAGCTTTCGCTTTCGCGGCTTTTTGCTCTGGcc                     >  1:1423335/1‑140 (MQ=255)                                                                                                                 tttttttttttTCC‑CCGCCGTTAGTAGCTACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAGTGTGCGGATCACCGCTGCCATGTATTCCTGAGCTTTCGCTTTCGCGGCTTTTTGCTCTGGCCCCt                  <  2:1218076/137‑1 (MQ=255)                                                                                                                  ttttttttttTCC‑CCGCCGTTAGTAGCTACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAGTGTGCGGATCACCGCTGCCATGTATTCCTGAGCTTTCGCTTTCGCGGCTTTTTGCTCTGGCCCCTg                 <  2:33711/138‑1 (MQ=255)                                                                                                                  ttttttttttCCC‑CCGCCGTTAGTAGCTACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAGTGTGCGGATCACCGCTGCCATGTATTCCTGAGCTTTCGCTTTCGCGGCTTTTTGCTCTGGCCCCTg                 <  1:353796/138‑1 (MQ=255)                                                                                                                  ttttttttttCCC‑CCGCCGTTAGTAGCTACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAGTGTGCGGATCACCGCTGCCATGTATTCCTGAGCTTTCGCTTTCGCGGCTTTTTGCTCTGGCCCCTg                 <  2:1423335/138‑1 (MQ=255)                                                                                                                    tttgtttTTCC‑CCGCCGTTAGTAGCTACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAGTGTGCGGATCACCGCTGCCATGTATTCCTGAGCTTTCGCTTTCGCGGCTTTTTGCTCTGGCCCCTGGa               >  1:648751/1‑140 (MQ=255)                                                                                                                    tttgtttTTCC‑CCGCCGTTAGTAGCTACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAGTGTGCGGATCACCGCTGCCATGTATTCCTGAGCTTTCGCTTTCGCGGCTTTTTGCTCTGGCCCCTGGa               >  2:276831/1‑140 (MQ=255)                                                                                                                    tttgtttTTCC‑CAGCCGTTAGTAGCTACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAGTGTGCGGATCACCGCTGCCATGTATTCCTGAGCTTTAGGTTTCTCGGCTTTTTGCTCTGATCCCTgca               >  2:1261214/1‑138 (MQ=255)                                                                                                                     tttttttcCC‑CCGCCGTTAGTAGCTACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAGTGTGCGGATCACCGCTGCCATGTATTCCTGAGCTTTCGCTTTCGCGGCTTTTTGCTCTGGCCCCTGGAt              <  1:553428/137‑1 (MQ=255)                                                                                                                      tgtttTTCC‑CCGCCGTTAGTAGCTACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAGTGTGCGGATCACCGCTGCCATGTATTCCTGGGCTTTCGCTTTCGCGGCTTTTTGCTCTGGCCCCTGGATg             >  1:630022/1‑140 (MQ=255)                                                                                                                      tgtttTTCC‑CCGCCGTTAGTAGCTACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAGTGTGCGGATCACCGCTGCCATGTATTCCTGAGCTTTCGCTTTCGCGGCTTTTTGCTCTGGCCCCTGGATg             >  2:14678/1‑140 (MQ=255)                                                                                                                            tCC‑CCGCCGTTAGTAGCTACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAGTGTGCGGATCACCGCTGCCATGTATTCCTGAGCTTTCGCTTTCGCGGCTTTTTGCTCTGGCCCCTGGATGGGTAGc       <  1:306265/140‑1 (MQ=255)                                                                                                                            tCC‑CCGCCGTTAGTAGCTACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAGTGTGCGGATCACCGCTGCCATGTATTCCTGAGCTTTCGCTTTCGCGGCTTTTTGCTCTGGCCCCTGGATGGGTAGc       <  2:108845/140‑1 (MQ=255)                                                                                                                            tCC‑CCGCCGTTAGTAGCTACTGCAGTTTTTTCTGGCTTTGTG‑TTTGGTCATTAATGGGGTGAGTGTGCGGATCACCGCTGCCATGTATTCCAGAGCTTTCGCTTTCGCGGCTTTTTGCTCTGGCCCCTGGATGGGTAGcc      >  2:447969/1‑140 (MQ=255)                                                                                                                             cc‑ccGCCGTTAGTAGCTACTGCAGTTTTTTCTGGATTTGTGTTTTGGCCATTAATGTGGTGAGAGTGCGGATCGCCGCTGCCATGTATTCCTGAGCTTTCGCTTTCGCGGCTTTTTGCTCTGGCCCCTGGGTGGGTAGcc      >  2:636125/1‑140 (MQ=255)                                                                                                                                  gccgTTAGTAGCTACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAGTGTGCGGATCACCGCTGCCATGTATTCCTGAGCTTTCGCTTTCGCGGCTTTTTGCTCTGGCCCCTGGATGGGTAGCCAGGt  <  2:540179/140‑1 (MQ=255)                                                                                                                                              |                                                                                                                                CAAGCGATTCAGATAGCGCCAGCTTAATCGGTTCAACAGCGAAGGTCAGCCCCTTTT‑CGCC‑GTTGTCC‑GCGACAACATAACGCAGTGCACCTTCTGTCTCGGTGTAATAACGTTTGTTTTTCC‑CCGCCGTTAGTAGCGACTGCAGTTTTTTCTGGCTTTGTGTTTTGGTCATTAATGGGGTGAGTGTGCGGATCACCGCTGCCATGTATTCCTGAGCTTTCGCTTTCGCGGCTTTTTGCTCTGGCCCCTGGATGGGTAGCCAGGT  >  NC_000913/4111632‑4111896

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑