Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,164,581 C→A Q315K (CAA→AAA)  btuB → cobalamin/cobinamide outer membrane transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,164,5810CA100.0% 343.4 / NA 98Q315K (CAA→AAA) btuBcobalamin/cobinamide outer membrane transporter
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (47/51);  total (47/51)

Only 100 of 105 total aligned reads displayed.

GTTGGGACGCCGGGCTGCGCTATAACGGCGAACTGATTAAATCACAACTCATTACCAGCTATAGCCATAGCAAAGATTACAACTACGATCCCCATTATGGTCGTTATGATTCGTCGGCGACGCTCGATGAGATGAAGCAATACACCGTCCAGTGGGCAAACAATGTCATCGTTGGTCACGGTAGTATTGGTGCGGGTGTCGACTGGCAGAAACAGACTACGACGCCGGGTACAGGTTATGTTGAGGATGGATATGATCAACGTAATACCGGCA  >  NC_000913/4164444‑4164716
                                                                                                                                         |                                                                                                                                       
gTTGGGACGCCGGGCTGCGCTATAACGGCGAACTGATTAAATCACAACTCATTACCAGCTATAGCCATAGCAAAGATTACAACTACGATCCCCATTATGGTCGTTATGATTCGTCGGCGACGCTCGATGAGATGAAGaaa                                                                                                                                       >  1:324321/1‑140 (MQ=255)
gTTGGGACGCCGGGCTGCGCTATAACGGCGAACTGATTAAATCACAACTCATTACCAGCTATAGCCATAGCAAAGATTACAACTACGATCCCCATTATGGTCGTTATGATTCGTCGGCGACGCTCGATGAGATGAAGaaa                                                                                                                                       >  1:709224/1‑140 (MQ=255)
  tGGGACGCCGGGCTGCGCTATAACGGCGAACTGATTAAATCACAACTCATTACCAGCTATAGCCATAGCAAAGATTACAACTACGATCCCCATTATGGTCGTTATGATTCGTCGGCGACGCTCGATGAGATGAAGAAATa                                                                                                                                     <  2:709224/140‑1 (MQ=255)
          cGGGCTGCGCTATAACGGCGAACTGATTAAATCACAACTCATTACCAGCTATAGCCATAGCAAAGATTACAACTACGATCCCCATTATGGTGGTTATGATTCTTCCGCGACGCTCGTTGAGATGAAGAAATACACCGTcc                                                                                                                             >  2:909086/1‑140 (MQ=255)
          cGGGCTGCGCTATAACGGCGAACTGATTAAATCACAACTCATTACCAGCTATAGCCATAGCAAAGATTACAACTACGATCCCCATTATGGTCGTTATGATTCGTCGGCGACGCTCGATGAGATGAAGAAATACCCCGTcc                                                                                                                             <  2:315505/140‑1 (MQ=255)
          cGGGCTGCGCTATAACGGCGAACTGATTAAATCACAACTCATTACCAGCTATAGCCATAGCAAAGATTACAACTACGATCCCCATTATGGTCGTTATGATTCGTCGGCGACGCTCGATGAGATGAAGAAATACACCGTcc                                                                                                                             <  1:568764/140‑1 (MQ=255)
                        aCGGCGAACTGATTAAATCACAACTCATTACCAGCTATAGCCATAGCAAAGATTACAACTACGATCCCCATTATGGTCGTTATGATTCGTCGGCGACGCTCGATGAGATGAAGAAATACACCGTCCAGTGGGCAAACAAt                                                                                                               <  2:1387909/140‑1 (MQ=255)
                           gCGAACTGATTAAATCACAACTCATTACCAGCTATAGCCATAGCAAAGATTACAACTACGATCCCCATTATGGTCGTTATGATTCGTCGGCGACGCTCGATGAGATGAAGAAATACACCGTCCAGTGGGCAAACAATGTc                                                                                                            >  1:1297608/1‑140 (MQ=255)
                           gCGAACTGATTAAATCACAACTCATTACCAGCTATAGCCATAGCAAAGATTACAACTACGATCCCCATTATGGTCGTTATGATTCGTCGGCGACGCTCGATGAGATGAAGAAATACACCGTCCAGTGGGCAAACAATGTc                                                                                                            >  2:1471609/1‑140 (MQ=255)
                           gCGAACTGATTAAATCACAACTCATTACCAGCTATAGCCATAGCAAAGATTACAACTACGATCCCCATTATGGTCGTTATGATTCGTCGGCGACGCTCGATGAGATGAAGAAATACACCGTCCAGTGGGCAAACAATGTc                                                                                                            >  2:1111293/1‑140 (MQ=255)
                              aaCTGATTAAATCACAACTCATTACCAGCTATAGCCATAGCAAAGATTACAACTACGATCCCCATTATGGTCGTTATGATTCGTCGGCGACGCTCGATGAGATGAAGAAATACACCGTCCAGTGGGCAAACAATGTCATc                                                                                                         >  1:930595/1‑140 (MQ=255)
                              aaCTGATTAAATCACAACTCATTACCAGCTATAGCCATAGCAAAGATTACAACTACGATCCCCATTATGGTCGTTATGATTCGTCGGCGACGCTCGATGAGATGAAGAAATACACCGTCCAGTGGGCAAACAATGTCATc                                                                                                         >  2:204196/1‑140 (MQ=255)
                               aCTGATTAAATCACAACTCATTACCAGCTATAGCCATAGCAAAGATTACAACTACGATCCCCATTATGGTCGTTATGATTCGTCGGCGACGCTCGATGAGATGAAGAAATACACCGTCCAGTGGGCAAACAATGTCATCg                                                                                                        <  2:952144/140‑1 (MQ=255)
                                cTGATTAAATCACAACTCATTACCAGCTATAGCCATAGCAAAGATTACAACTACGATCCCCATTATGGTCGTTATGATTCGTCGGCGACGCTCGATGAGATGAAGAAATACACCGTCCAGTGGGCAAACAATGTCATCGt                                                                                                       >  1:84612/1‑140 (MQ=255)
                                cTGATTAAATCACAACTCATTACCAGCTATAGCCATAGCAAAGATTACAACTACGATCCCCATTATGGTCGTTATGATTCGTCGGCGACGCTCGATGAGATGAAGAAATACACCGTCCAGTGGGCAAACAATGTCATCGt                                                                                                       >  1:618212/1‑140 (MQ=255)
                                    ttAAATCACAACTCATTACCAGCTATAGCCATAGCAAAGATTACAACTACGATCCCCATTATGGTCGTTATGATTCGTCGGCGACGCTCGATGAGATGAAGAAATACACCGTCCAGTGGGCAAACAATGTCATCGTTGGt                                                                                                   <  1:1210201/140‑1 (MQ=255)
                                     tAAATCACAACTCATTACCAGCTATAGCCATAGCAAAGATTACAACTACGATCCCCATTATGGTCGTTATGATTCGTCGGCGACGCTCGATGAGATGAAGAAATACACCGTCCAGTGGGCAAACAATGTCATCGTTGGTc                                                                                                  <  2:1245701/140‑1 (MQ=255)
                                     tAAATCACAACTCATTACCAGCTATAGCCATAGCAAAGATTACAACTACGATCCCCATTATGGTCGTTATGATTCGTCGGCGACGCTCGATGAGATGAAGAAATACACCGTCCAGTGGGCAAACAATGTCATCGTTGGTc                                                                                                  <  2:1067652/140‑1 (MQ=255)
                                     tAAATCACAACTCATTACCAGCTATAGCCATAGCAAAGATTACAACTACGATCCCCATTATGGTCGTTATGATTCGTCGGCGACGCTCGATGAGATGAAGAAATACACCGTCCAGTGGGCAAACAATGTCATCGTTGGTc                                                                                                  <  2:817565/140‑1 (MQ=255)
                                         tCACAACTCATTACCAGCTATAGCCATAGCAAAGATTACAACTACGATCCCCATTATGGTCGTTATGATTCGTCGGCGACGCTCGATGAGATGAAGAAATACACCGTCCAGTGGGCAAACAATGTCATCGTTGGTCACgg                                                                                              <  2:930595/140‑1 (MQ=255)
                                         tCACAACTCATTACCAGCTATAGCCATAGCAAAGATTACAACTACGATCCCCATTATGGTCGTTATGATTCGTCGGCGACGCTCGATGAGATGAAGAAATACACCGTCCAGTGGGCAAACAATGTCATCGTTGGTCACgg                                                                                              <  2:671027/140‑1 (MQ=255)
                                             aaCTCATTACCAGCTATAGCCATAGCAAAGATTACAACTACGATCCCCATTATGGTCGTTATGATTCGTCGGCGACGCTCGATGAGATGAAGAAATACACCGTCCAGTGGGCAAACAATGTCATCGTTGGTCACGgtagt                                                                                          >  1:16973/1‑140 (MQ=255)
                                             aaCTCATTACCAGCTATAGCCATAGCAAAGATTACAACTACGATCCCCATTATGGTCGTTATGATTCGTCGGCGACGCTCGATGAGATGAAGAAATACACCGTCCAGTGGGCAAACAATGTCATCGTTGGTCACGgtagt                                                                                          >  1:1221852/1‑140 (MQ=255)
                                                tCATTACCAGCTATAGCCATAGCAAAGATTACAACTACGATCCCCATTATGGTCGTTATGATTCGTCGGCGACGCTCGATGAGATGAAGAAATACACCGTCCAGTGGGCAAACAATGTCATCGTTGGTCACGGTAGTAtt                                                                                       <  2:200981/140‑1 (MQ=255)
                                                 cATTACCAGCTATAGCCATAGCAAAGATTACAACTACGATCCCCATTATGGTCGTTATGATTCGTCGGCGACGCTCGATGAGATGAAGAAATACACCGTCCAGTGGGCAAACAATGTCATCGTTGGTCACGGTAGTATTg                                                                                      >  1:550764/1‑140 (MQ=255)
                                                    tACCAGCTATAGCCATAGCAAAGATTACAACTACGATCCCCATTATGGTCGTTATGATTCGTCGGCGACGCTCGATGAGATGAAGAAATACACCGTCCAGTGGGCAAACAATGTCATCGTTGGTCACGGTAGTATTGGTg                                                                                   <  1:1471609/140‑1 (MQ=255)
                                                       cAGCTATAGCCATAGCAAAGATTACAACTACGATCCCCATTATGGTCGTTATGATTCGTCGGCGACGCTCGATGAGATGAAGAAATACACCGTCCAGTGGGCAAACAATGTCATCGTTGGTCACGGAAGTATTGGTGCgg                                                                                >  1:871873/1‑140 (MQ=255)
                                                          cTATAGCCATAGCAAAGATTACAACTACGATCCCCATTATGGTCGTTATGATTCGTCGGCGACGCTCGATGAGATGAAGAAATACACCGTCCAGTGGGCAAACAATGTCATCGTTGGTCACGGTAGTATTGGTGCGGgtg                                                                             >  2:748670/1‑140 (MQ=255)
                                                              aGCCATAGCAAAGATTACAACTACGATCCCCATTATGGTCGTTATGATTCGTCGGCGACGCTCGATGAGATGAAGAAATACACCGTCCAGTGGGCAAACAATGTCATCGTTGGTCACGGTAGTATTGGTGCGGGTGTCGa                                                                         >  1:157133/1‑140 (MQ=255)
                                                              aGCCATAGCAAAGATTACAACTACGATCCCCATTATGGTCGTTATGATTCGTCGGCGACGCTCGATGAGATGAAGAAATACACCGTCCAGTGGGCAAACAATGTCATCGTTGGTCACGGTAGTATTGGTGCGGGTGTCGa                                                                         <  1:1478363/140‑1 (MQ=255)
                                                              aGCCATAGCAAAGATTACAACTACGATCCCCATTATGGTCGTTATGATTCGTCGGCGACGCTCGATGAGATGAAGAAATACACCGTCCAGTGGGCAAACAATGTCATCGTTGGTCACGGTAGTATTGGTGCGGGTGTCGa                                                                         <  2:365539/140‑1 (MQ=255)
                                                                ccATAGCAAAGATTACAACTACGATCCCCATTATGGTCGTTATGATTCGTCGGCGACGCTCGATGAGATGAAGAAATACACCGTCCAGTGGGCAAACAATGTCATTGTTGGTCACGGTAGTATTGGTGCGGGTGTCGACt                                                                       <  2:651067/140‑1 (MQ=255)
                                                                   tAGCAAAGATTACAACTACGATCCCCATTATGGTCGTTATGATTCGTCGGCGACGCTCGATGAGATGAAGAAATACACCGTCCAGTGGGCAAACAATGTCATCGTTGGTCACGGTAGTATTGGTGCGGGTGTCGACTGGc                                                                    <  1:973664/140‑1 (MQ=255)
                                                                    aGCAAAGATTACAACTACGATCCCCATTATGGTCGTTATGATTCGTCGGCGACGCTCGATGAGATGAAGAAATACACCGTCCAGTGGGCAAACAATGTCATCGTTGGTCACGGTAGTATTGGTGCGGGTGTCGACTGGCa                                                                   >  2:893390/1‑140 (MQ=255)
                                                                    aGCAAAGATTACAACTACGATCCCCATTATGGTCGTTATGATTCGTCGGCGACGCTCGATGAGATGAAGAAATACACCGTCCAGTGGGCAAACAATGTCATCGTTGGTCACGGTAGTATTGGTGCGGGTGTCGACTGGCa                                                                   >  1:706727/1‑140 (MQ=255)
                                                                    aGCAAAGATTACAACTACGATCCCCATTATGGTCGTTATGATTCGTCGGCGACGCTCGATGAGATGAAGAAATACACCGTCCAGTGGGCAAACAATGTCATCGTTGGTCACGGTAGTATTGGTGCGGGTGTCGACTGGCa                                                                   >  1:1033707/1‑140 (MQ=255)
                                                                    aGCAAAGATTACAACTACGATCCCCATTATGGTCGTTATGATTCGTCGGCGACGCTCGATGAGATGAAGAAATACACCGTCCAGTGGGCAAACAATGTCATCGTTGGTCACGGTAGTATTGGTGCGGGTGTCGACTGGCa                                                                   >  1:702153/1‑140 (MQ=255)
                                                                    aGCAAAGATTACAACTACGATCCCCATTATGGTCGTTATGATTCGTCGGCGACGCTCGATGAGATGAAGAAATACACCGTCCAGTGGGCAAACAATGTCATCGTTGGTCACGGTAGTATTGGTGCGGGTGTCGACTGGCa                                                                   >  1:649082/1‑140 (MQ=255)
                                                                    aGCAAAGATTACAACTACGATCCCCATTATGGTCGTTATGATTCGTCGGCGACGCTCGATGAGATGAAGAAATACACCGTCCAGTGGGCAAACAATGTCATCGTTGGTCACGGTAGTATTGGTGCGGGTGTCGACTGGCa                                                                   >  1:252794/1‑140 (MQ=255)
                                                                    aGCAAAGATTACAACTACGATCCCCATTATGGTCGTTATGATTCGTCGGCGACGCTCGATGAGATGAAGAAATACACCGTCCAGTGGGCAAACAATGTCATCGTTGGTCACGGTAGTATTGGTGCGGGTGTCGACTGGCa                                                                   >  2:644725/1‑140 (MQ=255)
                                                                              aCAACTACGATCCCCATTATGGTCGTTATGATTCGTCGGCGACGCTCGATGAGATGAAGAAATACACCGTCCAGTGGGCAAACAATGTCATCGTTGGTCACGGTAGTATTGGTGCGGGTGTCGACTGGCAGAAACAGACt                                                         >  2:194489/1‑140 (MQ=255)
                                                                              aCAACTACGATCCCCATTATGGTCGTTATGATTCGTCGGCGACGCTCGATGAGATGAAGAAATACACCGTCCAGTGGGCAAACAATGTCATCGTTGGTCACGGTAGTATTGGTGCGGGTGTCGACTGGCAGAAACAGACt                                                         >  1:477347/1‑140 (MQ=255)
                                                                               cAACTACGATCCCCATTATGGTCGTTATGATTCGTCGGCGACGCTCGATGAGATGAAGAAATACACCGTCCAGTGGGCAAACAATGTCATCGTTGGTCACGGTAGTATTGGTGCGGGTGTCGACTGGCAGAAACAGACTa                                                        <  1:373623/140‑1 (MQ=255)
                                                                                aaCTACGATCCCCATTATGGTCGTTATGATTCGTCGGCGACGCTCGATGAGATGAAGAAATACACCGTCCAGTGGGCAAACAATGTCATCGTTGGTCACGGTAGTATTGGTGCGGGTGTCGACTGGCAGAAACAGACTac                                                       >  1:940070/1‑140 (MQ=255)
                                                                                aaCTACGATCCCCATTATGGTCGTTATGATTCGTCGGCGACGCTCGATGAGATGAAGAAATACACCGTCCAGTGGGCAAACAATGTCATCGTTGGTCACGGTAGTATTGGTGCGGGGGTCGACTGGCAGAAACAGACTac                                                       >  2:396808/1‑140 (MQ=255)
                                                                                 aCTACGATCCCCATTATGGTCGTTATGATTCGTCGGCGACGCTCGATGAGATGAAGAAATACACCGTCCAGTGGGCAAACAATGTCATCGTTGGTCACGGTAGTATTGGTGCGGGTGTCGACTGGCAGAAACAGACTacg                                                      <  1:624504/140‑1 (MQ=255)
                                                                                    aCGATCCCCATTATGGTCGTTATGATTCGTCGGCGACGCTCGATGAGATGAAGAAATACACCGTCCAGTGGGCAAACAATGTCATCGTTGGTCACGGTAGTATTGGTGCGGGTGTCGACTGGCAGAAACAGACTacgacg                                                   <  1:1357446/140‑1 (MQ=255)
                                                                                    aCGATCCCCATTATGGTCGTTATGATTCGTCGGCGACGCTCGATGAGATGAAGAAATACACCGTCCAGTGGGCAAACAATGTCATCGTTGGTCACGGTAGTATTGGTGCGGGTGTCGACTGGCAGAAACAGACTacgacg                                                   <  1:194489/140‑1 (MQ=255)
                                                                                    aCGATCCCCATTATGGTCGTTATGATTCGTCGGCGACGCTCGATGAGATGAAGAAATACACCGTCCAGTGGGCAAACAATGTCATCGTTGGTCACGGTAGTATTGGTGCGGGTGTCGACTGGCAGAAACAGACTacgacg                                                   <  2:1033707/140‑1 (MQ=255)
                                                                                     cGATCGCCATTATGGTCGTTATGATTCGTCGGCGACGCTCGATGAGATGAAGAAATACACCGTCCAGTGGGCAAACAATGTCATTGTTGGTCACGGTAGTATTGGTGCGGGTGTCGACTGGCAGAAACAGACTACGACGc                                                  >  2:216655/1‑140 (MQ=255)
                                                                                     cGATCCCCATTATGGTCGTTATGATTCGTCGGCGACGCTCGATGAGATGAAGAAATACACCGTCCAGTGGGCAAACAATGTCATCGTTGGTCACGGTAGTATTGGTGCGGGTGTCGACTGGCAGAAACAGACTACGACGc                                                  >  2:567522/1‑140 (MQ=255)
                                                                                     cGATCCCCATTATGGTCGTTATGATTCGTCGGCGACGCTCGATGAGATGAAGAAATACACCGTCCAGTGGGCAAACAATGTCATCGTTGGTCACGGTAGTATTGGTGCGGGTGTCGACTGGCAGAAACAGACTACGACGc                                                  >  2:867762/1‑140 (MQ=255)
                                                                                     cGATCCCCATTATGGTCGTTATGATTCGTCGGCGACGCTCGATGAGATGAAGAAATACACCGTCCAGTGGGCAAACAATGTCATCGTTGGTCACGGTAGTATTGGTGCGGGTGTCGACTGGCAGAAACAGACTACGACGc                                                  >  1:121086/1‑140 (MQ=255)
                                                                                     cGATCCCCATTATGGTCGTTATGATTCGTCGGCGACGCTCGATGAGATGAAGAAATACACCGTCCAGTGGGCAAACAATGTCATCGTTGGTCACGGTAGTATTGGTGCGGGTGTCGACTGGCAGAAACAGACTACGACGc                                                  >  1:1447369/1‑140 (MQ=255)
                                                                                     cGATCCCCATTATGGTCGTTATGATTCGTCGGCGACGCTCGATGAGATGAAGAAATACACCGTCCAGTGGGCAAACAATGTCATCGTTGGTCACGGTAGTATTGGTGCGGGTGTCGACTGGCAGAAACAGACTACGACGc                                                  >  1:1073238/1‑140 (MQ=255)
                                                                                      gATCCCCATTATGGTCGTTATGATTCGTCGGCGACGCTCGATGAGATGAAGAAATTCACCGTCCAGTGGGCAAACAATGTCATCGTTGGTCACGGTAGTATTGGTGCGGGTGTCGACTGGCAGAAACAGACTACGGCGcc                                                 <  2:1023208/140‑1 (MQ=255)
                                                                                      gATCCCCATTATGGTCGTTATGATTCGTCGGCGACGCTCGATGAGATGAAGAAATACACCGTCCAGTGGGCAAACAATGTCATCGTTGGTCACGGTAGTATTGGTGCGGGTGTCGACTGGCAGAAACAGACTACGACGcc                                                 <  2:1283896/140‑1 (MQ=255)
                                                                                      gATCCCCATTATGGTCGTTATGATTCGTCGGCGACGCTCGATGAGATGAAGAAATACACCGTCCAGTGGGCAAACAATGTCATCGTTGGTCACGGTAGTATTGGTGCGGGTGTCGACTGGCAGAAACAGACTACGACGcc                                                 <  2:1002439/140‑1 (MQ=255)
                                                                                      gATCCCCATTATGGTCGTTATGATTCGTCGGCGACGCTCGATGAGATGAAGAAATACACCGTCCAGTGGGCAAACAATGTCATCGTTGGTCACGGTAGTATTGGTGCGGGTGTCGACTGGCAGAAACAGACTACGACGcc                                                 >  1:674651/1‑140 (MQ=255)
                                                                                          cccATTATGGTCGTTATGATTCGTCGGCGACGCTCGATGAGATGAAGAAATACACCGTCCAGTGGGCAAACAATGTCATCGTTGGTCACGGTAGTATTGGTGCGGGTGTCGACTGGCAGAAACAGACTACGACGCCGGGt                                             >  1:63688/1‑140 (MQ=255)
                                                                                          cccATTATGGTCGTTATGATTCGTCGGCGACGCTCGATGAGATGAAGAAATACACCGTCCAGTGGGCAAACAATGTCATCGTTGGTCACGGTAGTATTGGTGCGGGTGTCGACTGGCAGAAACAGACTACGACGCCGGGt                                             >  1:1093235/1‑140 (MQ=255)
                                                                                            cATTATGGTCGTTATGATTCGTCGGCGACGCTCGATGAGATGAAGAAATACACCGTCCAGTGGGCAAACAATGTCATCGTTGGTCACGGTAGTATTGGTGCGGGTGTCGACTGGCAGAAACAGACTacgacg                                                   >  2:95089/1‑132 (MQ=255)
                                                                                            cATTATGGTCGTTATGATTCGTCGGCGACGCTCGATGAGATGAAGAAATACACCGTCCAGTGGGCAAACAATGTCATCGTTGGTCACGGTAGTATTGGTGCGGGTGTCGACTGGCAGAAACAGACTacgacg                                                   <  1:95089/132‑1 (MQ=255)
                                                                                            cATTATGGTCGTTATGATTCGTCGGCGACGCTCGATGAGATGAAGAAATACACCGTCCAGTGGGCAAACAATGTCATCGTTGGTCACGGTAGTATTGGTGCGGGTGTCGACTGGCAGAAACAGACTACGACGCCGGGTAc                                           >  2:864166/1‑140 (MQ=255)
                                                                                             aTTATGGTCGTTATGATTCGTCGGCGACGCTCGATGAGATGAAGAAATACACCGTCCAGTGGGCAAACAATGTCATCGTTGGTCACGGTAGTATTGGTGCGGGTGTCGACTGGCAGAAACAGACTacgacg                                                   <  2:478813/131‑1 (MQ=255)
                                                                                             aTTATGGTCGTTATGATTCGTCGGCGACGCTCGATGAGATGAAGAAATACACCGTCCAGTGGGCAAACAATGTCATCGTTGGTCACGGTAGTATTGGTGCGGGTGTCGACTGGCAGAAACAGACTacgacg                                                   >  1:478813/1‑131 (MQ=255)
                                                                                               tatcctCGTTACGATTCGTCGGCGCCGCTCGATGAGATGAAGAAATACACCGTCCAGTGGGCAAACAATGTCATCGTTGGTCACGGTAGTATTGGTGCGGGTGTCGACTGGCAGAAACAGACTACGACGCCGGGTACAgg                                        <  2:242897/135‑1 (MQ=255)
                                                                                               tATGGTCGTTATGATTCGTCGGCGACGCTCGATGAGATGAAGAAATACACCGTCCAGTGGGCAAACAATGTCATCGTTGGTCACGGTAGTATTGGTGCGGGTGTCGACTGGCAGAAACAGACTACGACGCCGGGTACAgg                                        >  1:277271/1‑140 (MQ=255)
                                                                                                aTGGTCGTTATGATTCGTCGGCGACGCTCGATGAGATGAAGAAATACACCGTCCAGTGGGCAAACAATGTCATCGTTGGTCACGGTAGTATTGGTGCGGGTGTCGACTGGCAGAAACAGACTACGACGCCGGGTACAGGt                                       <  2:1221852/140‑1 (MQ=255)
                                                                                                 tGGTCGTTATGATTCGTCGGCGACGCTCGATGAGATGAAGAAATACACCGTCCAGTGGGCAAACAATGTCATCGTTGGTCACGGTAGTATTGGTGCGGGTGTCGACTGGCAGAAACAGACTACGACGCCGGGTACAGGtt                                      <  1:204196/140‑1 (MQ=255)
                                                                                                    tCGTTATGATTCGTCGGCGACGCTCGATGAGATGAAGAAATACACCGTCCAGTGGGCAAACAATGTCATCGTTGGTCACGGTAGTATTGGTGCGGGTGTCGACTGGCAGAAACAGACTACGACGCCGGGt                                             <  1:774508/130‑1 (MQ=255)
                                                                                                    tCGTTATGATTCGTCGGCGACGCTCGATGAGATGAAGAAATACACCGTCCAGTGGGCAAACAATGTCATCGTTGGTCACGGTAGTATTGGTGCGGGTGTCGACTGGCAGAAACAGACTACGACGCCGGGt                                             >  2:774508/1‑130 (MQ=255)
                                                                                                    tCGTTATGATTCGTCGGCGACGCTCGATGAGATGAAGAAATACACCGTCCAGTGGGCAAACAATGTCATCGTTGGTCACGGTAGTATTGGTGCGGGTGTCGACTGGCAGAAACAGACTACGACGCCGGGTACAGGTTATg                                   >  2:214086/1‑140 (MQ=255)
                                                                                                    tCGTTATGATTCGTCGGCGACGCTCGATGAGATGAAGAAATACACCGTCCAGTGGGCAAACAATGTCATCGTTGGTCACGGTAGTATTGGTGCGGGTGTCGACTGGCAGAAACAGACTACGACGCCGGGTACAGGTTATg                                   >  1:1323433/1‑140 (MQ=255)
                                                                                                      gTTATGATTCGTCGGCGACGCTCGATGAGATGAAGAAATACACCGTCCAGTGGGCAAACAATGTCATCGTTGGTCACGGTAGTATTGGTGCGGGTGTCGACTGGCAGAAACAGACTACGACGCCGGGTACAGGTTATGtt                                 <  2:75350/140‑1 (MQ=255)
                                                                                                       ttATGATTCGTCGGCGACGCTCGATGAGATGAAGAAATACACCGTCCAGTGGGCAAACAATGTCATCGTTGGTCACGGTAGTATTGGTGCGGGTGTCGACTGGCAGAAACAGACTACGACGCCGGGTACAGGTTATGTTg                                <  2:4192/140‑1 (MQ=255)
                                                                                                        tATGATTCGTCGGCGACGCTCGATGAGATGAAGAAATACACCGTCCAGTGGGCAAACAATGTCATCGTTGGTCACGGTAGTATTGGTGCGGGTGTCGACTGGCAGAAACAGACTACGACGCCGGGTACAGGTTATGTTGa                               >  2:747489/1‑140 (MQ=255)
                                                                                                        tATGATTCGTCGGCGACGCTCGATGAGATGAAGAAATACACCGTCCAGTGGGCAAACAATGTCATCGTTGGTCACGGTAGTATTGGTGCGGGTGTCGACTGGCAGAAACAGACTACGACGCCGGGTACAGGTTATGTTGa                               >  2:783250/1‑140 (MQ=255)
                                                                                                        tATGATTCGTCGGCGACGCTCGATGAGATGAAGAAATACACCGTCCAGTGGGCAAACAATGTCATCGTTGGTCACGGTAGTATTGGTGCGGGTGTCGACTGGCAGAAACAGACTACGACGCCGGGTACAGGTTATGTTGa                               >  1:1222127/1‑140 (MQ=255)
                                                                                                         aTGATTCGTCGGCGACGCTCGATGAGATGAAGAAATACACCGTCCAGTGGGCAAACAATGTCATCGTTGGTCACGGTAGTATTGGTGCGGGTGTCGACTGGCAGAAACAGACTACGACGCCGGGTACAGGTTATGTTGAg                              >  1:322033/1‑140 (MQ=255)
                                                                                                          tGATTCGTCGGCGACGCTCGATGAGATGAAGAAATACACCGTCCAGTGGGCAAACAATGTCATCGTTGGTCACGGTAGTATTGGTGCGGGTGTCGACTGGCAGAAACAGACTACGACGCCGGGTACAGGTTATGTTGAgg                             <  2:1323433/140‑1 (MQ=255)
                                                                                                          tGATTCGTCGGCGACGCTCGATGAGATGAAGAAATACACCGTCCAGTGGGCAAACAATGTCATCGTTGGTCACGGTAGTATTGGTGCGGGTGTCGACTGGCAGAAACAGACTACGACGCCGGGTACAGGTTATGTTGAgg                             <  2:706727/140‑1 (MQ=255)
                                                                                                          tGATTCGTCGGCGACGCTCGATGAGATGAAGAAATACACCGTCCAGTGGGCAAACAATGTCATCGTTGGTCACGGTAGTATTGGTGCGGGTGTCGACTGGCAGAAACAGACTACGACGCCGGGTACAGGTTATGTTGAgg                             <  2:121086/140‑1 (MQ=255)
                                                                                                           gATTCGTCGGCGACGCTCGATGAGATGAAGAAATACCCCGTCCAGTGGGCAAACAATGTCATCGTTGGTCACGGTAGTATTGGTGCGGGTGTCGACTGGCAGAAACAGACTACGACGCCGGGTACAGGTTATGTTGAgga                            <  1:909086/140‑1 (MQ=255)
                                                                                                           gATTCGTCGGCGACGCTCGATGAGATGAAGAAATACACCGTCCAGTGGGCAAACAATGTCATCGTTGGTCACGGTAGTATTGGTGCGGGTGTCGACTGGCAGAAACAGACTACGACGCCGGGTACAGGTTATGTTGAgga                            <  2:477347/140‑1 (MQ=255)
                                                                                                                gtcgGCGACGCTCGATGAGATGAAGAAATACACCGTCCAGTGGGCAAACAATGTCATCGTTGGTCACGGTAGTATTGGTGCGGGTGTCGACTGGCAGAAACAGACTACGACGCCGGGTACAGGTTATGTTGAggatggat                       <  2:550764/140‑1 (MQ=255)
                                                                                                                gtcgGCGACGCTCGATGAGATGAAGAAATACACCGTCCAGTGGGCAAACAATGTCATCGTTGGTCACGGTAGTATTGGTGCGGGTGTCGACTGGCAGAAACAGACTACGACGCCGGGTACAGGTTATGTTGAggatggat                       <  1:646556/140‑1 (MQ=255)
                                                                                                                   ggCGACGCTCGATGAGATGAAGAAATACACCGTCCAGTGGGCAAACAATGTCATCGTTGGTCACGGTAGTATTGGTGCGGGTGTCGACTGGCAGAAACAGACTACGACGCCGGGTACAGGTTATGTTGAGGATGGATATg                    <  1:1111293/140‑1 (MQ=255)
                                                                                                                     cGACGCTCGATGAGATGAAGAAATACACCGTCCAGTGGGCAAACAATGTCATCGTTGGTCACGGTAGTATTGGTGCGGGTGTCGACTGGCAGAAACAGACTACGACGCCGGGTACAGGTTATGTTGAGGATGGATATGAt                  <  1:214086/140‑1 (MQ=255)
                                                                                                                     cGACGCTCGATGAGATGAAGAAATACACCGTCCAGTGGGCAAACAATGTCATCGTTGGTCACGGTAGTATTGGTGCGGGTGTCGACTGGCAGAAACAGACTACGACGCCGGGTACAGGTTATGTTGAGGATGGATATGAt                  <  2:324321/140‑1 (MQ=255)
                                                                                                                     cGACGCTCGATGAGATGAAGAAATACACCGTCCAGTGGGCAAACAATGTCATCGTTGGTCACGGTAGTATTGGTGCGGGTGTCGACTGGCAGAAACAGACTACGACGCCGGGTACAGGTTATGTTGAGGATGGATATGAt                  <  2:84612/140‑1 (MQ=255)
                                                                                                                     cGACGCTCGATGAGATGAAGAAATACACCGTCCAGTGGGCAAACAATGTCATCGTTGGTCACGGTAGTATTGGTGCGGGTGTCGACTGGCAGAAACAGACTACGACGCCGGGTACAGGTTATGTTGAGGATGGATATGAt                  <  2:157133/140‑1 (MQ=255)
                                                                                                                     cGACGCTCGATGAGATGAAGAAATACACCGTCCAGTGGGCAAACAATGTCATCGTTGGTCACGGTAGTATTGGTGCGGGTGTCGACTGGCAGAAACAGACTACGACGCCGGGTAAAGGTTATGTTGAAGATGGATATGac                  <  2:16973/140‑2 (MQ=255)
                                                                                                                      gACGCTCGATGAGATGAAGAAATACACCGTCCAGTGGGCAAACAATGTCATCGTTGGTCACGGTAGTATTGATGCGGGTGTCGACTGGCAGAAACAGACTACGACGCCGGGTACAGGTTATGTTGAGGATGGATATGATc                 <  2:848597/140‑1 (MQ=255)
                                                                                                                                gagatgaAGAAATACACCGTCCAGTGGGCAAACAATGTCATCGTTGGTCACGGTAGTATTGGTGCGGGTGTCGACTGGCAGAAACAGACTACGACGCCGGGTACAGGTTATGTTGAGGATGGATATGATCAACGTAATAc       <  2:252794/140‑1 (MQ=255)
                                                                                                                                  gatgaAGAAATACACCGTCCAGTGGGCAAACAATGTCATCGTTGGTCACGGTAGTATTGGTGCGGGTGTCGACTGGCAGAAACAGACTACGACGCCGGGTACAGGTTATGTTGAGGATGGATATGATCAACGTAATACCg     >  1:1041624/1‑140 (MQ=255)
                                                                                                                                  gatgaAGAAATACACCGTCCAGTGGGAAAACAATGTCATCGTTGGTCACGGTAGTATTGGTGCGGGTGTCGACTGGCAGAAACAGACTACGACGCCGGGTACAGGTTATGTTGAGGATGGATATGATCAACGTAATACCg     >  1:403073/1‑140 (MQ=255)
                                                                                                                                    tgaAGAAATACACCGTCCAGTGGGCAAACAATGTCATCGTTGGTCACGGTAGTATTGGTGCGGGTGTCGACTGGCAGAAACAGACTACGACGCCGGGTACAGGTTATGTTGAGGATGGATATGATCAACGTAATACCGGc   <  1:783250/140‑1 (MQ=255)
                                                                                                                                    tgaAGAAATACACCGTCCAGTGGGCAAACAATGTCATCGTTGGTCACGGTAGTATTGGTGCGGGTGTCGACTGGCAGAAACAGACTACGACGCCGGGTACAGGTTATGTTGAGGATGGATATGATCAACGTAATACCGGc   <  2:1073238/140‑1 (MQ=255)
                                                                                                                                     gaagaaATACACCGTCCAGTGGGCAAACAATGTCATCGTTGGTCACGGTAGTATTGGTGCGGGTGTCGACTGGCAGAAACAGACTACGACGCCGGGTACAGGTTATGTTGAGGATGGATATGATCAACGTAATACCGGCa  <  1:396808/140‑1 (MQ=255)
                                                                                                                                         |                                                                                                                                       
GTTGGGACGCCGGGCTGCGCTATAACGGCGAACTGATTAAATCACAACTCATTACCAGCTATAGCCATAGCAAAGATTACAACTACGATCCCCATTATGGTCGTTATGATTCGTCGGCGACGCTCGATGAGATGAAGCAATACACCGTCCAGTGGGCAAACAATGTCATCGTTGGTCACGGTAGTATTGGTGCGGGTGTCGACTGGCAGAAACAGACTACGACGCCGGGTACAGGTTATGTTGAGGATGGATATGATCAACGTAATACCGGCA  >  NC_000913/4164444‑4164716

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑