Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,215,728	C→A	P84H (CCT→CAT)	aceB →	malate synthase A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,215,728	0	C	A	100.0%	303.3 / NA	84	P84H (CCT→CAT)	aceB	malate synthase A
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (34/50);  total (34/50)

GCATTTTACGCCACAACGCAATAAACTTCTGGCAGCGCGCATTCAGCAGCAGCAAGATATTGATAACGGAACGTTGCCTGATTTTATTTCGGAAACAGCTTCCATTCGCGATGCTGATTGGAAAATTCGCGGGATTCCTGCGGACTTAGAAGACCGCCGCGTAGAGATAACTGGCCCGGTAGAGCGCAAGATGGTGATCAACGCGCTCAACGCCAATGTGAAAGTCTTTATGGCCGATTTCGAAGATTCACTGGCACCAGACTGGA  >  NC_000913/4215591‑4215856                                                                                                                                          |                                                                                                                                 gCATTTTACGCCACAACGCAATAAACTTCTGGCAGCGCGCATTCAGCAGTAGCAAGATATTGATAACGGAACGTTGCCTGATTTTATTTCGGAAACAGCTTCCATTCGCGATGCTGATTGGAAAATTCGCGGGATTCATg                                                                                                                                <  2:677196/140‑1 (MQ=255) gCATTTTACGCCACAACGCAATAAACTTCTGGCAGCGCGCATTCAGCAGCAGCAAGATATTGATAACGGAACGTTGCCTGATTTTATTTCGGAAACAGCTTCCATTCGCGATGCTGATTGGAAAATTCGCGGGATTCATg                                                                                                                                >  1:1105286/1‑140 (MQ=255) gCATTTTACGCCACAACGCAATAAACTTCTGGCAGCGCGCATTCAGCAGCAGCAAGATATTGATAACGGAACGTTGCCTGATTTTATTTCGGAAACAGCTTCCATTCGCGATGCTGATTGGAAAATTCGCGGGATTCATg                                                                                                                                >  1:1190820/1‑140 (MQ=255) gCATTTTACGCCACAACGCAATAAACTTCTGGCAGCGCGCATTCAGCAGCAGCAAGATATTGATAACGGAACGTTGCCTGATTTTATTTCGGAAACAGCTTCCATTCGCGATGCTGATTGGAAAATTCGCGGGATTCATg                                                                                                                                >  1:374217/1‑140 (MQ=255) gCATTTTACGCCACAACGCAATAAACTTCTGGCAGCGCGCATTCAGCAGCAGCAAGATATTGATAACGGAACGTTGCCTGATTTTATTTCGGAAACAGCTTCCATTCGCGATGCTGATTGGAAAATTCGCGGGATTCATg                                                                                                                                <  2:520888/140‑1 (MQ=255)  cATTTTACGCCACAACGCAATAAACTTCTGGCAGCGCGCATTCAGCAGCAGCAAGATATTGATAACGGAACGTTGCCTGATTTTATTTCGGAAACAGCTTCCATTCGCGATGCTGATTGGAAAATTCGCGGGATTCATGc                                                                                                                               <  1:997863/140‑1 (MQ=255)  cATTTTACGCCACAACGCAATAAACTTCTGGCAGCGCGCATTCAGCAGCAGCAAGATATTGATAACGGAACGTTGCCTGATTTTATTTCGGAAACAGCTTCCATTCGCGATGCTGATTGGAAAATTCGCGGGATTCATGc                                                                                                                               <  2:803390/140‑1 (MQ=255)    ttttACGCCACAACGCAATAAACTTCTGGCAGCGCGCATTCAGCAGCAGCAAGATATTGATAACGGAACGTTGCCTGATTTTATTTCGGAAACAGCTTCCATTCGCGATGCTGATTGGAAAATTCGCGGGATTCATGCgg                                                                                                                             <  2:1016487/140‑1 (MQ=255)    ttttACGCCACAACGCAATAAACTTCTGGCAGCGCGCATTCAGCAGCAGCAAGATATTGATAACGGAACGTTGCCTGATTTTATTTCGGAAACAGCTTCCATTCGCGATGCTGATTGGAAAATTCGCGGGATTCATGCgg                                                                                                                             <  1:966722/140‑1 (MQ=255)    ttttACGCCACAACGCAATAAACTTCTGGCAGCGCGCATTCAGCAGCAGCAAGATATTGATAACGGAACGTTGCCTGATTTTATTTCGGAAACAGCTTCCATTCGCGATGCTGATTGGAAAATTCGCGGGATTCATGCgg                                                                                                                             <  1:1386092/140‑1 (MQ=255)    ttttACGCCACAACGCAATAAACTTCTGGCAGCGCGCATTCAGCAGCAGCAAGATATTGATAACGGAACGTTGCCTGATTTTATTTCGGAAACAGCTTCCATTCGCGATGCTGATTGGAAAATTCGCGGGATTCATGCgg                                                                                                                             <  2:1190820/140‑1 (MQ=255)       tACGCCACAACGCAATAAACTTCTGGCAGCGCGCATTCAGCAGCAGCAAGATATTGATAACGGAACGTTGCCTGATTTTATTTCGGAAACAGCTTCCATTCGCGATGCTGATTGGAAAATTCGCGGGATTCATGCGGACt                                                                                                                          <  1:692165/140‑1 (MQ=255)              caACGCAATAAACTTCTGGCAGCGCGCATTCAGCAGCAGCAAGATATTGATAACGGAACGTTGCCTGATTTTATTTCGGAAACAGCTTCCATTCGCGATGCTGATTGGAAAATTCGCGGGGTTCATGCGGACTTagaaga                                                                                                                   >  2:858801/1‑140 (MQ=255)               aaCGCAATAAACTTCTGGCAGCGCGCATTCAGCAGCAGCAAGATATTGATAACGGAACGTTGCCTGATTTTATTTCGGAAACAGCTTCCATTCGCGATGCTGATTGGAAAATTCGCGGGATTCATGCGGACTTAGAAGAc                                                                                                                  >  2:255605/1‑140 (MQ=255)               aaCGCAATAAACTTCTGGCAGCGCGCATTCAGCAGCAGCAAGATATTGATAACGGAACGTTGCCTGATTTTATTTCGGAAACAGCTTCCATTCGCGATGCTGATTGGAAAATTCGCGGGATTCATGCGGACTTAGAAGAc                                                                                                                  >  2:1336418/1‑140 (MQ=255)                 cGCAATAAACTTCTGGCAGCGCGCATTCAGCAGCAGCAAGATATTGATAACGGAACGTTGCCTGATTTTATTTCGGAAACAGCTTCCATTCGCGATGCTGATTGGAAAATTCGCGGGATTCATGCGGACTTAGAAGAccg                                                                                                                <  2:1022116/140‑1 (MQ=255)                 cGCAATAAACTTCTGGCAGCGCGCATTCAGCAGCAGCAAGATATTGATAACGGAACGTTGCCTGATTTTATTTCGGAAACAGCTTCCATTCGCGATGCTGATTGGAAAATTCGCGGGATTCATGCGGACTTAGAAGAccg                                                                                                                >  1:1279810/1‑140 (MQ=255)                 cGCAATAAACTTCTGGCAGCGCGCATTCAGCAGCAGCAAGATATTGATAACGGAACGTTGCCTGATTTTATTTCGGAAACAGCTTCCATTCGCGATGCTGATTGGAAAATTCGCGGGATTCATGCGGACTTAGAAGAccg                                                                                                                <  2:745844/140‑1 (MQ=255)                  gCAATAAACTTCTGGCAGCGCGCATTCAGCAGCAGCAAGATATTGATAACGGAACGTTGCCTGATTTTATTTCGGAAACAGCTTCCATTCGCGATGCTGATTGGAAAATTCGCGGGATTCATGCGGACTTAGAAGAccgc                                                                                                               <  1:581962/140‑1 (MQ=255)                     aTAAACTTCTGGCAGCGCGCATTCAGCAGCAGCAAGATATTGATAACGGAACGTTGCCTGATTTTATTTCGGAAACAGCTTCCATTCGCGATGCTGATTGGAAAATTCGCGGGATTCATGCGGACTTAGAAGACcgccgc                                                                                                            <  1:147768/140‑1 (MQ=255)                     aTAAACTTCTGGCAGCGCGCATTCAGCAGCAGCAAGATATTGATAACGGAACGTTGCCTGATTTTATTTCGGAAACAGCTTCCATTCGCGATGCTGATTGGAAAATTCGCGGGATTCATGCGGACTTAGAAGACcgccgc                                                                                                            <  2:1437036/140‑1 (MQ=255)                     aTAAACTTCTGGCAGCGCGCATTCAGCAGCAGCAAGATATTGATAACGGAACGTTGCCTGATTTTATTTCGGAAACAGCTTCCATTCGCGATGCTGATTGGAAAATTCGCGGGATTCATGCGGACTTAGAAGACcgccgc                                                                                                            <  1:872463/140‑1 (MQ=255)                      tAAACTTCTGGCAGCGCGCATTCAGCAGCAGCAAGATATTGATAACGGAACGTTGCCTGATTTTATTTCGGAAACAGCTTCCATTCGCGATGCTGATTGGAAAATTCGCGGGATTCATGCGGACTTAGAAGACCGCcgcg                                                                                                           <  2:1279810/140‑1 (MQ=255)                      tAAACTTCTGGCAGCGCGCATTCAGCAGCAGCAAGATATTGATAACGGAACGTTGCCTGATTTTATTTCGGAAACAGCTTCCATTCGCGATGCTGATTGGAAAATTCGCGGGATTCATGC‑GACTTAGAAGACCGCCGCGt                                                                                                          >  1:144317/1‑140 (MQ=255)                         aCTTCTGGCAGCGCGCATTCAGCAGCAGCAAGATATTGATAACGGAACGTTGCCTGATTTTATTTCGGAAACAGCTTCCATTCGCGATGCTGATTGGAAAATTCGCGGGATTCATGCGGACTTAGAAGACCGCCGCGTag                                                                                                        >  2:930213/1‑140 (MQ=255)                              tGGCAGCGCGCATTCAGCAGCAGCAAGATATTGATAACGGAACGTTGCCTGATTTTATTTCGGAAACAGCTTCCATTCGCGATGCTGATTGGAAAATTCGCGGGATTCATGCGGACTTAGAAGACCGCCGCGTAGAGATa                                                                                                   <  2:320021/140‑1 (MQ=255)                                gCAGCGCGCATTCAGCAGCAGCAAGATATTGATAACGGAACGTTGCCTGATTTTATTTCGGAAACAGCTTCCATTCGCGATGCTGATTGGAAAATTCGCGGGATTCATGCGGACTTAGAAGACCGCCGCGTAGAGATAAc                                                                                                 <  2:1105286/140‑1 (MQ=255)                                gCAGCGCGCATTCAGCAGCAGCAAGATATTGATAACGGAACGTTGCCTGATTTTATTTCGGAAACAGCTTCCATTCGCGATGCTGATTGGAAAATTCGCGGGATTCATGCGGACTTAGAAGACCGCCGCGTAGAGATAAc                                                                                                 <  2:290759/140‑1 (MQ=255)                                gCAGCGCGCATTCAGCAGCAGCAAGATATTGATAACGGAACGTTGCCTGATTTTATTTCGGAAACAGCTTCCATTCGCGATGCTGATTGGAAAATTCGCGGGATTCATGCGGACTTAGAAGACCGCCGCGTAGAGATAAc                                                                                                 <  1:710158/140‑1 (MQ=255)                                    cgcgcATTCAGCAGCAGCAAGATATTGATAACGGAACGTTGCCTGATTTTATTTCGGAAACAGCTTCCATTCGCGATGCTGATTGGAAAATTCGCGGGATTCATGCGGACTTAGAAGACCGCCGCGTAGAGATAAc                                                                                                 >  2:357418/1‑136 (MQ=255)                                    cgcgcATTCAGCAGCAGCAAGATATTGATAACGGAACGTTGCCTGATTTTATTTCGGAAACAGCTTCCATTCGCGATGCTGATTGGAAAATTCGCGGGATTCATGCGGACTTAGAAGACCGCCGCGTAGAGATAAc                                                                                                 <  1:357418/136‑1 (MQ=255)                                    cgcgcATTCAGCAGCAGCAAGATATTGATAACGGAACGTTGCCTGATTTTATTTCGGAAACAGCTTCCATTCGCGATGCTGATTGGAAAATTCGCGGGATTCATGCGGACTTAGAAGACCGCCGCGTAGAGATAACTGGc                                                                                             <  2:374217/140‑1 (MQ=255)                                       gcATTCAGCAGCAGCAAGATATTGATAACGGAACGTTGCCTGATTTTATTTCGGAAACAGCTTCCATTCGCGATGCTGATTGGAAAATTCGCGGGATTCATGCGGACTTAGAAGACCGCCGCGTAGAGATAACTGGCCCg                                                                                          <  1:391184/140‑1 (MQ=255)                                          ttCAGCAGCAGCAAGATATTGATAACGGAACGTTGCCTGATTTTATTTCGGAAACAGCTTCCATTCGCGATGCTGATTGGAAAATTCGCGGGATTCATGCGGACTTAGAAGACCGCCGCGTAGAGATAACTGGCCCGGTa                                                                                       <  1:619011/140‑1 (MQ=255)                                          ttCAGCAGCAGCAAGATATTGATAACGGAACGTTGCCTGATTTTATTTCGGAAACAGCTTCCATTCGCGATGCTGATTGGAAAATTCGCGGGATTCATGCGGACTTAGAAGACCGCCGCGTAGAGATAACTGGCCCGGTa                                                                                       <  1:51172/140‑1 (MQ=255)                                          ttCAGCAGCAGCAAGATATTGATAACGGAACGTTGCCTGATTTTATTTCGGAAACAGCTTCCATTCGCGATGCTGATTGGAAAATTCGCGGGATTCATGCGGACTTAGAAGACCGCCGCGTAGAGATAACTGGCCCGGTa                                                                                       <  2:21362/140‑1 (MQ=255)                                          ttCAGCAGCAGCAAGATATTGATAACGGAACGTTGCCTGATTTTATTTCGGAAACAGCTTCCATTCGCGATGCTGATTGGAAAATTCGCGGGATTCATGCGGACTTAGAAGACCGCCGCGTAGAGATAACTGGCCCGGTa                                                                                       <  1:499371/140‑1 (MQ=255)                                               catcagcaAGATATTGATAACGGAACGTTGGCTGATTTTATTTCGGAAACAGCTTCCATTCGCGATGCTGATTGGAAAAATCGCGGGATTCATGCGGACTTAGAAGACCGCCGCGTAGAGATAACTGGCCCGGTAGAgcg                                                                                  <  2:77309/137‑1 (MQ=255)                                               cagcagcaAGATATTGATAACGGAACGTTGCCTGATTTTATTTCGGAAACAGCTTCCATTCGCGATGCTGATTGGAAAATTCGCGGGATTCATGCGGACTTAGAAGACCGCCGCGTAGAGATAACTGGCCCGGTAGAgcg                                                                                  <  1:321953/140‑1 (MQ=255)                                               cagcagcaAGATATTGATAACGGAACGTTGCCTGATTTTATTTCGGAAACAGCTTCCATTCGCGATGCTGATTGGAAAATTCGCGGGATTCATGCGGACTTAGAAGACCGCCGCGTAGAGATAACTGGCCCGGTAGAgcg                                                                                  <  1:385196/140‑1 (MQ=255)                                                 gcagcaAGATATTGATAACGGAACGTTGCCTGATTTTATTTCGGAAACAGCTTCCATTCGCGATGCTGATTGGAAAATTCGCGGGATTCATGCGGACTTAGAAGACCGCCGCGTAGAGATAACTGGCCCGGTAGAGCGCa                                                                                <  1:641058/140‑1 (MQ=255)                                                  cagcaAGATATTGATAACGGAACGTTGCCTGATTTTATTTCGGAAACAGCTTCCATTCGCGATGCTGATTGGAAAATTCGCGGGATTCATGCGGACTTAGAAGACCGCCGCGTAGAGATAACTGGCCCGGTAGAGCGCaa                                                                               >  1:1322907/1‑140 (MQ=255)                                                   agcaAGATATTGATAACGGAACGTTGCCTGATTTTATTTCGGAAACAGCTTCCATTCGCGATGCTGATTGGAAAATTCGCGGGATTCATGCGGACTTAGAAGACCGCCGCGTAGAGATAACTGGCCCGGTAGAGCGCAAg                                                                              >  1:1210433/1‑140 (MQ=255)                                                   agcaAGATATTGATAACGGAACGTTGCCTGATTTTATTTCGGAAACAGCTTCCATTCGCGATGCTGATTGGAAAATTCGCGGGATTCATGCGGACTTAGAAGACCGCCGCGTAGAGATAACTGGCCCGGTAGAGCGCAAg                                                                              <  1:547869/140‑1 (MQ=255)                                                   agcaAGATATTGATAACGGAACGTTGCCTGATTTTATTTCGGAAACAGCTTCCATTCGCGATGCTGATTGGAAAATTCGCGGGATTCATGCGGACTTAGAAGACCGCCGCGTAGAGATAACTGGCCCGGTAGAGCGCAAg                                                                              <  1:1420683/140‑1 (MQ=255)                                                     caAGATATTGATAACGGAACGTTGCCTGATTTTATTTCGGAAACAGCTTCCATTCGCGATGCTGATTGGAAAATTCGCGGGATTCATGCGGACTTAGAAGACCGCCGCGTAGAGATAACTGGCCCGGTAGAGCGCAAGAt                                                                            <  2:60614/140‑1 (MQ=255)                                                     caAGATATTGATAACGGAACGTTGCCTGATTTTATTTCGGAAACAGCTTCCATTCGCGATGCTGATTGGAAAATTCGCGGGATTCATGCGGACTTAGAAGACCGCCGCGTAGAGATAACTGGCCCGGTAGAGCGCAAGAt                                                                            >  2:662450/1‑140 (MQ=255)                                                     caAGATATTGATAACGGAACGTTGCCTGATTTTATTTCGGAAACAGCTTCCATTCGCGATGCTGATTGGAAAATTCGCGGGATTCATGCGGACTTAGAAGACCGCCGCGTAGAGATAACTGGCCCGGTAGAGCGCAAGAt                                                                            >  1:1240134/1‑140 (MQ=255)                                                       aGATATTGATAACGGAACGTTGCCTGATTTTATTTCGGAAACAGCTTCCATTCGCGATGCTGATTGGAAAATTCGCGGGATTCATGCGGACTTAGAAGACCGCCGCGTAGAGATAACTGGCCCGGTAGAGCGCAAGATgg                                                                          >  2:607158/1‑140 (MQ=255)                                                        gATATTGATAACGGAACGTTGCCTGATTTTATTTCGGAAACAGCTTCCATTCGCGATGCTGATTGGAAAATTCGCGGGATTCATGCGGACTTAGAAGACCGCCGCGTAGAGATAACTGGCCCGGTAGAGCGCAAGATGGt                                                                         >  2:753242/1‑140 (MQ=255)                                                               aTAACGGAACGTTGCCTGATTTTATTTCGGAAACAGCTTCCATTCGCGATGCTGATTGGAAAATTCGCGGGATTCATGCGGACTTAGAAGACCGCCGCGTAGAGATAACTGGCCCGGTAGAGCGCAAGATGGTGATCAAc                                                                  >  2:580943/1‑140 (MQ=255)                                                               aTAACGGAACGTTGCCTGATTTTATTTCGGAAACAGCTTCCATTCGCGATGCTGATTGGAAAATTCGCGGGATTCATGCGGACTTAGAAGACCGCCGCGTAGAGATAACTGGCCCGGTAGAGCGCAAGATGGTGATCAAc                                                                  >  1:1185367/1‑140 (MQ=255)                                                               aTAACGGAACGTTGCCTGATTTTATTTCGGAAACAGCTTCCATTCGCGATGCTGATTGGAAAATTCGCGGGATTCATGCGGACTTAGAAGACCGCCGCGTAGAGATAACTGGCCCGGTAGAGCGCAAGATGGTGATCAAc                                                                  >  1:572028/1‑140 (MQ=255)                                                                tAACGGAACGTTGCCTGATTTTATTTCGGAAACAGCTTCCATTCGCGATGCTGATTGGAAAATTCGCGGGATTCATGCGGACTTAGAAGACCGCCGCGTAGAGATAACTGGCCCGGTAGAGCGCAAGATGGTGATCAAcg                                                                 <  1:1336418/140‑1 (MQ=255)                                                                  aCGGAACGTTGCCTGATTTTATTTCGGAAACAGCTTCCATTCGCGATGCTGATTGGAAAATTCGCGGGATTCATGCGGACTTAGAAGACCGCCGCGTAGAGATAACTGGCCCGGTAGAGCGCAAGATGGTGATCAAcgcg                                                               >  2:1293062/1‑140 (MQ=255)                                                                  aCGGAACGTTGCCTGATTTTATTTCGGAAACAGCTTCCATTCGCGATGCTGATTGGAAAATTCGCGGGATTCATGCGGACTTAGAAGACCGCCGCGTAGAGATAACTGGCCCGGTAGAGCGCAAGATGGTGATCAAcgcg                                                               >  2:1426677/1‑140 (MQ=255)                                                                  aCGGAACGTTGCCTGATTTTATTTCGGAAACAGCTTCCATTCGCGATGCTGATTGGAAAATTCGCGGGATTCATGCGGACTTAGAAGACCGCCGCGTAGAGATAACTGGCCCGGTAGAGCGCAAGATGGTGATCAAcg                                                                 <  2:947486/138‑1 (MQ=255)                                                                  aCGGAACGTTGCCTGATTTTATTTCGGAAACAGCTTCCATTCGCGATGCTGATTGGAAAATTCGCGGGATTCATGCGGACTTAGAAGACCGCCGCGTAGAGATAACTGGCCCGGTAGAGCGCAAGATGGTGATCAAcg                                                                 >  1:947486/1‑138 (MQ=255)                                                                   cGGAACGTTGCCTGATTTTATTTCGGAAACAGCTTCCATTCGCGATGCTGATTGGAAAATTCGCGGGATTCATGCGGACTTAGAAGACCGCCGCGTAGAGATAACTGGCCCGGTAGAGCGCAAGATGGTGATCAACgcgc                                                              >  1:1216480/1‑140 (MQ=255)                                                                   cGGAACGTTGCCTGATTTTATTTCGGAAACAGCTTCCATTCGCGATGCTGATTGGAAAATTCGCGGGATTCATGCGGACTTAGAAGACCGCCGCGTAGAGATAACTGGCCCGGTAGAGCGCAAGATGGTGATCAACgcgc                                                              >  2:75761/1‑140 (MQ=255)                                                                   cGGAACGTTGCCTGATTTTATTTCGGAAACAGCTTCCATTCGCGATGCTGATTGGAAAATTCGCGGGATTCATGCGGACTTAGAAGACCGCCGCGTAGAGATAACTGGCCCGGTAGAGCGCAAGATGGTGATCAACgcgc                                                              >  1:153638/1‑140 (MQ=255)                                                                   cGGAACGTTGCCTGATTTTATTTCGGAAACAGCTTCCATTCGCGATGCTGATTGGAAAATTCGCGGGATTCATGCGGACTTAGAAGACCGCCGCGTAGAGATAACTGGCCCGGTAGAGCGCAAGATGGTGATCAACgcgc                                                              >  1:429465/1‑140 (MQ=255)                                                                      aaCGTTGCCTGATTTTATTTCGGAAACAGCTTCCATTCGCGATGCTGATTGGAAAATTCGCGGGATTCATGCGGACTTAGAAGACCGCCGCGTAGAGATAACTGGCCCGGTAGAGCGCAAGATGGTGATCAACGCGCTCa                                                           >  1:167841/1‑140 (MQ=255)                                                                          ttGCCTGATTTTATTTCGGAAACAGCTTCCATTCGCGATGCTGATTGGAAAATTCGCGGGATTCATGCGGACTTAGAAGACCGCCGCGTAGAGATAACTGGCCCGGTAGAGCGCAAGATGGTGATCAACGCGCTCAACGc                                                       <  2:1111070/140‑1 (MQ=255)                                                                           tGCCTGATTTTATTTCGGAAACAGCTTCCATTCGCGATGCTGATTGGAAAATTCGCGGGATTCATGCGGACTTAGAAGACCGCCGCGTAGAGATAACTGGCCCGGTAGAGCGCAAGATGGTGATCAACGCGCTCAACGcc                                                      >  2:92828/1‑140 (MQ=255)                                                                            gCCTGATTTTATTTCGGAAACAGCTTCCATTCGCGATGCTGATTGGAAAATTCGCGGGATTCATGCGGACTTAGAAGACCGCCGCGTAGAGATAACTGGCCCGGTAGAGCGCAAGATGGTGATCAACGCGCTCAACGCCa                                                     <  1:607158/140‑1 (MQ=255)                                                                               tGATTTTATTTCGGAAACAGCTTCCATTCGCGATGCTGATTGGAAAATTCGCGGGATTCATGCGGACTTAGAAGACCGCCGCGTAGAGATAACTGGCCCGGTAGAGCGCAAGATGGTGATCAACGCGCTCAACGCCAAtg                                                  <  2:824399/140‑1 (MQ=255)                                                                                  ttttATTTCGGAAACAGCTTCCATTCGCGATGCTGATTGGAAAATTCGCGGGATTCATGCGGACTTAGAAGACCGCCGCGTAGAGATAACTGGCCCGGTAGAGCGCAAGATGGTGATCAACGCGCTCAACGCCAATGTGa                                               <  2:153638/140‑1 (MQ=255)                                                                                  ttttATTTCGGAAACAGCTTCCATTCGCGATGCTGATTGGAAAATTCGCGGGATTCATGCGGACTTAGAAGACCGCCGCGTAGAGATAACTGGCCCGGTAGAGCGCAAGATGGTGATCAACGCGCTCAACGCCAATGTGa                                               <  1:1409430/140‑1 (MQ=255)                                                                                       tttCGGAAACAGCTTCCATTCGCGATGCTGATTGGAAAATTCGCGGGATTCATGCGGACTTAGAAGACCGCCGCGTAGAGATAACTGGCCCGGTAGAGCGCAAGATGGTGATCAACGCGCTCAACGCCAATGTGaaa                                             >  2:1401609/1‑137 (MQ=255)                                                                                       tttCGGAAACAGCTTCCATTCGCGATGCTGATTGGAAAATTCGCGGGATTCATGCGGACTTAGAAGACCGCCGCGTAGAGATAACTGGCCCGGTAGAGCGCAAGATGGTGATCAACGCGCTCAACGCCAATGTGaaa                                             <  1:1401609/137‑1 (MQ=255)                                                                                       tttCGGAAACAGCTTCCATTCGCGATGCTGATTGGAAAATTCGCGGGATTCATGCGGACTTAGAAGACCGCCGCGTAGAGATAACTGGCCCGGTAGAGCGCAAGATGGTGATCAACGCGCTCAACGCCAATGTGAAAGTc                                          >  2:55624/1‑140 (MQ=255)                                                                                               aCAGCTTCCATTCGCGATGCTGATTGGAAAATTCGCGGGGTTCATGCGGACTTAGAAGACCGCCGCGTAGAGATAACTGGCCCGGTAGAGCGCAAGATGGTGATCAACGCGCTCAACGCCAATGTGAAAGTCTTTATGGc                                  <  1:858801/140‑1 (MQ=255)                                                                                                           cgcgATGCTGATTGGAAAATTCGCGGGATTCATGCGGACTTAGAAGACCGCCGCGTAGAGATAACTGGCCCGGTAGAGCGCAAGATGGTGATCAACGCGCTCAACGCCAATGTGAAAGTCTTTATGGCCGATTTCGAAGa                      >  1:64434/1‑140 (MQ=255)                                                                                                           cgcgATGCTGATTGGAAAATTCGCGGGATTCATGCGGACTTAGAAGACCGCCGCGTAGAGATAACTGGCCCGGTAGAGCGCAAGATGGTGATCAACGCGCTCAACGCCAATGTGAAAGTCTTTATGGCCGATTTCGAAGa                      >  2:729301/1‑140 (MQ=255)                                                                                                           cgcgATGCTGATTGGAAAATTCGCGGGATTCATGCGGACTTAGAAGACCGCCGCGTAGAGATAACTGGCCCGGTAGAGCGCAAGATGGTGATCAACGCGCTCAACGCCAATGTGAAAGTCTTTATGGCCGATTTCGAAGa                      >  1:696470/1‑140 (MQ=255)                                                                                                            gcgATGCTGATTGGAAAATTCGCGGGATTCATGCGGACTTAGAAGACCGCCGCGTAGAGATAACTGGCCCGGTAGAGCGCAAGATGGTGATCAACGCGCTCAACGCCAATGTGAAAGTCTTTATGGCCGATTTCGAAGAt                     <  2:1216480/140‑1 (MQ=255)                                                                                                               aTGCTGATTGGAAAATTCGCGGGATTCATGCGGACTTAGAAGACCGCCGCGTAGAGATAACTGGCCCGGTAGAGCGCAAGATGGTGATCAACGCGCTCAACGCCAATGTGAAAGTCTTTATGGCCGATTTCGAAGATTCa                  <  1:753242/140‑1 (MQ=255)                                                                                                                      ttGGAAAATTCGCGGGATTCATGCGGACTTAGAAGTCCGCGGTGTAGAGATAACTGGGCCGGTAGCGAGCCAGATGGTGATCAACGCGCTCAACGCCAATGTGAAAGTCTTTATGGCCGATTTCGAAGATTCACTGGCAc           <  2:167841/140‑1 (MQ=255)                                                                                                                      ttGGAAAATTCGCGGGATTCATGCGGACTTAGAAGACCGCCGCGTAGAGATAACTGGCCCGGTAGAGCGCAAGATGGTGATCAACGCGCTCAACGCCAATGTGAAAGTCTTTATGGCCGATTTCGAAGATTCACTGGCAc           <  2:1366231/140‑1 (MQ=255)                                                                                                                        ggAAAATTCGCGGGATTCATGCGGACTTAGAAGACCGCCGCGTAGAGATAACTGGCCCGGTAGAGCGCAAGATGGTGATCAACGCGCTCAACGCCAATGTGAAAGTCTTTATGGCCGATTTCGAAGATTCACTGGCACCa         <  2:415814/140‑1 (MQ=255)                                                                                                                          aaaaTTCGCGGGATTCATGCGGACTTAGAAGACCGCCGCGTAGAGATAACTGGCCCGGTAGAGCGCAAGATGGTGATCAACGCGCTCAACGCCAATGTGAAAGTCTTTATGGCCGATTTCGAAGATTCACTGGCACCAGa       <  1:255605/140‑1 (MQ=255)                                                                                                                          aaaaTTCGCGGGATTCATGCGGACTTAGAAGACCGCCGCGTAGAGATAACTGGCCCGGTAGAGCGCAAGATGGTGATCAACGCGCTCAACGCCAATGTGAAAGTCTTTATGGCCGATTTCGAAGATTCACTGGCACCAGa       <  1:930213/140‑1 (MQ=255)                                                                                                                               tCGCGGGATTCATGCGGACTTAGAAGACCGCCGCGTAGAGATAACTGGCCCGGTAGAGCGCAAGATGGTGATCAACGCGCTCAACGCCAATGTGAAAGTCTTTATGGCCGATTTCGAAGATTCACTGGCACCAGACTGGa  <  1:92828/140‑1 (MQ=255)                                                                                                                               tCGCGGGATTCATGCGGACTTAGAAGACCGCCGCGTAGAGATAACTGGCCCGGTAGAGCGCAAGATGGTGATCAACGCGCTCAACGCCAATGTGAAAGTCTTTATGGCCGATTTCGAAGATTCACTGGCACCAGACTGGa  >  1:1254550/1‑140 (MQ=255)                                                                                                                               tCGCGGGATTCATGCGGACTTAGAAGACCGCCGCGTAGAGATAACTGGCCCGGTAGAGCGCAAGATGGTGATCAACGCGCTCAACGCCAATGTGAAAGTCTTTATGGCCGATTTCGAAGATTCACTGGCACCAGACTGGa  <  2:1240134/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                 GCATTTTACGCCACAACGCAATAAACTTCTGGCAGCGCGCATTCAGCAGCAGCAAGATATTGATAACGGAACGTTGCCTGATTTTATTTCGGAAACAGCTTCCATTCGCGATGCTGATTGGAAAATTCGCGGGATTCCTGCGGACTTAGAAGACCGCCGCGTAGAGATAACTGGCCCGGTAGAGCGCAAGATGGTGATCAACGCGCTCAACGCCAATGTGAAAGTCTTTATGGCCGATTTCGAAGATTCACTGGCACCAGACTGGA  >  NC_000913/4215591‑4215856

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑