Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,568,5040TG37.4% 337.9 / 38.5 181T6P (ACC→CCC) mdtMmultidrug efflux pump/bile salt:H(+) antiporter/Na(+):H(+) antiporter/K(+):H(+) antiporter
Reads supporting (aligned to +/- strand):  ref base T (25/87);  new base G (67/0);  total (94/87)
Fisher's exact test for biased strand distribution p-value = 1.87e-28
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 189 total aligned reads displayed.

GGGCCAGACTGACATCGGCATTAAAATCACGTACCACATTAATGATCCCAGGCTGGATCAGATCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGG‑TAAAAAAACGTGG‑CATAACAAACAACTCCTTGTCCGGA‑G‑AA‑CCCCAACGGGGAAAACGAAATTCAATACCGTCGGTAATGACGAAAGGCAGGTGAGAAGGGCAACGAGACTGCAAATGTACTGAATTTCATGTAATCAAC  >  NC_000913/4568369‑4568643
                                                                                                                                        |                                                                                                                                               
  gCCAGACTGACATCGGCATTAAAATCACGTACCACATTAATGATCCCAGGCTGGATCAGATCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGG‑Taaaaaa                                                                                                                                           >  2:2877923/1‑140 (MQ=255)
   ccAGACTGACATCGGCATTAAAATCACGTACCACATTAATGATCCCAGGCTGGATCAGATCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGG‑Taaaaaaa                                                                                                                                          <  2:938788/140‑1 (MQ=255)
     aGACTGACATCGGCATTAAAATCACGTACCACATTAATGATCCCAGGCTGGATCAGATCCGTCGACAGATACGCAGCAAAGTTATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGG‑TAAAAAAACg                                                                                                                                        <  2:1018674/140‑1 (MQ=255)
     aGACTGACATCGGCATTAAAATCACGTACCACATTAATGATCCCAGGCTGGATCAGATCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGG‑TAAAAAAACg                                                                                                                                        <  2:2445820/140‑1 (MQ=255)
       aCTGACATCGGCATTAAAATCACGTACCACATTAATGATCCCAGGCTGGATCAGATCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGG‑Taaaaaaa                                                                                                                                          <  2:703634/136‑1 (MQ=255)
       aCTGACATCGGCATTAAAATCACGTACCACATTAATGATCCCAGGCTGGATCAGATCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGG‑TAAAAAAACGTg                                                                                                                                      <  1:2665969/140‑1 (MQ=255)
       aCTGACATCGGCATTAAAATCACGTACCACATTAATGATCCCAGGCTGGATCAGATCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGG‑Gaaaaaaa                                                                                                                                          >  1:703634/1‑136 (MQ=255)
          gACATCGGCATTAAAATCACGTACCACATTAATGATCCCAGGCTGGATCAGATCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGG‑TAAAAAAACGTGG‑Ca                                                                                                                                  >  2:629334/1‑140 (MQ=255)
          gACATCGGCATTAAAATCACGTACCACATTAATGATCCCAGGCTGGATCAGATCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCAACGGGAAAAACAGCGTGGCGGCATGGCGGG‑TAAAAAAACGTGG‑Ca                                                                                                                                  <  2:943053/140‑1 (MQ=255)
            cATCGGCATTAAAATCACGTACCACATTAATGATCCCAGGCTGGATCAGATCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGG‑TAAAAAAACGTGG‑CATa                                                                                                                                >  1:858385/1‑140 (MQ=255)
             aTCGGCATTAAAATCACGTACCACATTAATGATCCCAGGCTGGATCAGATCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGG‑TAAAAAAACGTGG‑CATaa                                                                                                                               <  1:1009780/140‑1 (MQ=255)
             aTCGGCATTAAAATCACGTACCACATTAATGATCCCAGGCTGGATCAGATCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGG‑TAAAAAAACGTGG‑CATaa                                                                                                                               <  1:2877923/140‑1 (MQ=255)
                 gCATTAAAATCACGTACCACATTAATGATCCCAGGCTGGATCAGATCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGG‑TAAAAAAACGTGG‑CATaacaaa                                                                                                                           <  2:1832841/140‑1 (MQ=255)
                 gCATTAAAATCACGTACCACATTAATGATCCCAGGCTGGATCAGATCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGG‑GAAAAAAAAGCGG‑CCTaacaaa                                                                                                                           >  2:7585/1‑140 (MQ=255)
                   aTTAAAATCACGTACCACATTAATGATCCCAGGCTGGATCAGATCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGG‑TAAAAAAACGTGG‑CATaacaaaca                                                                                                                         <  1:2347364/140‑1 (MQ=255)
                   aTTAAAATCACGTACCACATTAATGATCCCAGGCTGGATCAGATCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGG‑GAAAAAAACGTGG‑CATaacaaaca                                                                                                                         >  1:1082807/1‑140 (MQ=255)
                        aaTCACGTACCACATTAATGATCCCAGGCTGGATCAGATCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGG‑TAAAAAAACGTGG‑CATAACAAACAACTcc                                                                                                                    <  1:2556443/140‑1 (MQ=255)
                             cGTACCACATTAATGATCCCAGGCTGGATCAGATCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGG‑TAAAAAAACGTGG‑CATAACAAACAACTCCTTGTc                                                                                                               >  2:1535313/1‑140 (MQ=255)
                               tACCACATTAATGATCCCAGGCTGGATCAGATCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGG‑GAAAAAAAAAAGG‑GATAACAAAAAACTCCCTTTccc                                                                                                             >  1:2862719/1‑139 (MQ=255)
                                   acaTTAATGATCCCAGGCTGGATCAGATCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGG‑CAAAAAAAAAAGG‑CAATAAAAACAACAACTTTTcctgcct                                                                                                         >  2:2683206/1‑135 (MQ=255)
                                    caTTAATGATCCCAGGCTGGATCAGATCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGG‑TAAAAAAAAGTGG‑CATAACAAAAAACTCCTTTTCCCGG‑G‑aa                                                                                                      >  2:2426299/1‑140 (MQ=255)
                                    caTTAATGATCCCAGGCTGGATCAGATCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGG‑GAAAAAAAAGGGG‑CATAAAAAACAACCCCTTTTCCGga‑gga                                                                                                       >  2:521426/1‑138 (MQ=255)
                                    caTTAATGATCCCAGGCTGGATCAGATCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGG‑GAAAAAAAAGAGG‑GATAAAAAAAAACACCCTTTCCGga‑gga                                                                                                       >  2:562131/1‑138 (MQ=255)
                                    caTTAATGATCCCAGGCTGGATCAGATCCGTCGACAGATACGCAGCAAAGTCATACAAAATAAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGG‑GAAAAAAAAGGGG‑GCTAAAAAAAAAATCCCTTTCCGGA‑G‑aa                                                                                                      >  2:1985183/1‑140 (MQ=255)
                                    caTTAATGACCCCAGGCTGGATCAGATCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGG‑TAAAAAAACGTGG‑CATAACAAACAACTCCTTGTCCGGA‑G‑aa                                                                                                      >  2:1082330/1‑140 (MQ=255)
                                         aTGATCCCAGGCTGGATCAGATCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGG‑TAAAAAAACGTGG‑CATAACAAACAACTCCTTGTCCGGA‑G‑AA‑CCCCa                                                                                                <  1:1340214/140‑1 (MQ=255)
                                            aTCCCAGGCTGGATCAGATCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGG‑GAAAAAAAAGTGG‑GATAAAAAACAACTCCTTGTCCGGA‑G‑AA‑ACCCAACg                                                                                             >  1:1089663/1‑140 (MQ=255)
                                            aTCCCAGGCTGGATCAGATCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGG‑GAAAAAAAAGTGG‑CATAACAAACAACTCCTTGTCCGGA‑G‑AA‑CCCCAACg                                                                                             >  1:830215/1‑140 (MQ=255)
                                            aTCCCAGGCTGGATCAGATCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGG‑GAAAAAAAAATGG‑CCTAAAAAACAACCCCTTTTCCGGG‑G‑GA‑CCCCAACg                                                                                             >  2:728724/1‑140 (MQ=255)
                                              cccAGGCTGGATCAGATCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGG‑GAAAAAAAAATGG‑CATAAAAAAAAACTCCCTGGCCGGA‑G‑AA‑ACCCaaaagg                                                                                           >  2:2262222/1‑136 (MQ=255)
                                               ccAGGCTGGATCAGATCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGG‑TAAAAAAACGTGG‑CATAACAAACAACTCCTTGTCCGGA‑G‑AA‑CCCCAACgggg                                                                                          >  1:2263544/1‑140 (MQ=255)
                                               ccAGGCTGGATCAGATCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGG‑TAAAAAAAAGAGG‑CATAAAAAAAAACACCTTGTCCGGA‑G‑AA‑TCCCCACcggg                                                                                          >  2:2881751/1‑140 (MQ=255)
                                               ccAGGCTGGATCAGATCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGG‑GAAAAAAACGAGG‑GACAACAAACAAATCCTTGTCCCGA‑G‑AA‑ACCCAACgggg                                                                                          >  1:2548322/1‑140 (MQ=255)
                                               ccAGGCTGGATCAGATCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGG‑GAAAAAAAAGTGG‑CATAAAAAACAACTCCTTTTCCGGA‑G‑AA‑CCCCAACgggg                                                                                          >  1:555830/1‑140 (MQ=255)
                                               ccAGGCTGGATCAGATCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGG‑GAAAAAAAAGTGG‑CATAAAAAAAAACCCCTTGTCCGGG‑G‑AA‑CCCCAACgggg                                                                                          >  2:283894/1‑140 (MQ=255)
                                               ccAGGCTGGATCAGATCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGG‑GAAAAAAAAGTGG‑CATAAAAAAAAACCCCTTGTCCGGA‑G‑AA‑CCCCAACgggg                                                                                          >  2:2085206/1‑140 (MQ=255)
                                               ccAGGCTGGATCAGATCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGG‑GAAAAAAAAGTGG‑CATAAAAAAAAAATCCCTGTCCGGA‑G‑AA‑CCCCCACaggg                                                                                          >  2:2611853/1‑140 (MQ=255)
                                               ccAGGCTGGATCAGATCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGG‑GAAAAAAAAGTGG‑CATAAAAAAAAAACCCCTTTCCGGA‑G‑AA‑ACCCCACgggg                                                                                          >  2:295637/1‑140 (MQ=255)
                                               ccAGGCTGGATCAGATCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGG‑GAAAAAAAAATGG‑CATAACAAAAAACCCCCTGTCCGGA‑G‑AA‑CCCCCACgggg                                                                                          >  2:140875/1‑140 (MQ=255)
                                               ccAGGCTGGATCAGATCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGG‑GAAAAAAAAAAGG‑CATAAAAAAAAACTCCTTGTCCGGG‑G‑AA‑ACCCAACgggg                                                                                          >  2:2226357/1‑140 (MQ=255)
                                                 aGGCTGGATCAGATCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGCAAAAAAAAAGTGG‑‑ATAAAAAAAAAATCCTTGTGCGGG‑G‑AAGCCCAAACGGGGa                                                                                         >  2:1498342/1‑140 (MQ=255)
                                                 aGGCTGGATCAGATCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGG‑TAAAAAAACGTGG‑CATAACAAACAACTCCTTGTCCGGA‑G‑AA‑CCCCAACGGGGaa                                                                                        >  1:1123327/1‑140 (MQ=255)
                                                   gCTGGATCAGATCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGG‑TAAAAAAACGTGG‑CATAACAAACAACTCCTTGTCCGGA‑G‑AA‑CCCCAACGGGGaaaa                                                                                      <  1:117671/140‑1 (MQ=255)
                                                   gCTGGATCAGATCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGG‑GAAAAAAAAGTGG‑CATAACAAAAAACTCCTTGTCCGGG‑G‑AA‑CCCCCACGGGGgaaa                                                                                      >  2:1680506/1‑140 (MQ=255)
                                                   gCTGGATCAGATCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGG‑GAAAAAAAAGTGG‑CATAAAAAAAAACACCTTGTCCGGA‑G‑AA‑ACCCAACAGGGgaaa                                                                                      >  2:1753906/1‑140 (MQ=255)
                                                     tGGATCAGATCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGG‑TAAAAAAACGTGG‑CATAACAAACAACTCCTTGTCCGGA‑G‑AA‑CCCCAACGGGGAAAACg                                                                                    <  1:1309562/140‑1 (MQ=255)
                                                     tGGATCAGATCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGG‑TAAAAAAACGTGG‑CATAACAAACAACTCCTTGTCCGGA‑G‑AA‑CCCCAACGGGGAAAACg                                                                                    <  2:1123327/140‑1 (MQ=255)
                                                     tGGATCAGATCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGG‑TAAAAAAACGTGG‑CATAACAAACAACTCCTTGTCCGGA‑G‑AA‑CCCCAACGGGGAAAACg                                                                                    <  1:980568/140‑1 (MQ=255)
                                                     tGGATCAGATCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGG‑TAAAAAAACGTGG‑CATAACAAACAACTCCTTGTCCGGA‑G‑AA‑CCCCAACGGGGAAAACg                                                                                    <  1:624735/140‑1 (MQ=255)
                                                     tGGATCAGATCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGG‑TAAAAAAACGTGG‑CATAACAAACAACTCCTTGTCCGGA‑G‑AA‑CCCCAACGGGGAAAACg                                                                                    <  1:2419609/140‑1 (MQ=255)
                                                      ggATCAGATCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGG‑GAAAAAAACGTGG‑CATAACAAACAACTCCTTGTCCGGA‑G‑AA‑CCCCAACGGGGAAAACGa                                                                                   >  1:2218326/1‑140 (MQ=255)
                                                       gATCAGATCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGG‑TAAAAAAACGTGG‑CATAACAAACAACTCCTTGTCCGGA‑G‑AA‑CCCCAACGGGGAAAACGaa                                                                                  <  1:2426299/140‑1 (MQ=255)
                                                       gATCAGATCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGG‑GAAAAAAAAGTGG‑GATAAAAAAAAACTCCTTGTCCGGA‑G‑AA‑ACCCAACGGGGGAAACGaa                                                                                  >  1:2466172/1‑140 (MQ=255)
                                                       gATCAGATCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGG‑GAAAAAAAAGTGG‑CATAACAAAAAACTCCTTTTCCGGA‑G‑AA‑CCCCCACGGGGGAAAAGaa                                                                                  >  1:1514245/1‑140 (MQ=255)
                                                       gATCAGATCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGG‑GAAAAAAAAGGGG‑CATAAAAAACAACTCCTTGTCCGGG‑G‑AA‑CCCCCACCGGGAAAACGaa                                                                                  >  2:117671/1‑140 (MQ=255)
                                                       gATCAGATCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGG‑GAAAAAAAAGCGG‑GCAAACAAAAAACTCCTTGTTCGGAGG‑AA‑CCCCAAGGGGGaaaaaaa                                                                                   >  2:1796320/1‑137 (MQ=255)
                                                        aTCAGATCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGG‑TGAAAAAAAGCGG‑CACAAAAAAAAAACCGCTTTACGGG‑G‑AA‑CCCCAACGGGGGgaaagaaa                                                                                 >  2:2575401/1‑140 (MQ=255)
                                                        aTCAGATCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGG‑TAAAAAAAAGAGG‑CATAAAAAAAAACTCCTTGTCCGGA‑G‑AA‑CCCCAACGGGGGAAACGaaa                                                                                 >  2:634567/1‑140 (MQ=255)
                                                        aTCAGATCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGG‑GAAAAAAAAGTGG‑CATAACAAACAACACCTTTTCCGGA‑G‑AA‑ACCCCACGGGGGAAAAGaaa                                                                                 >  2:2547785/1‑140 (MQ=255)
                                                        aTCAGATCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGG‑GAAAAAAAAAAGGCCAAAAAAAAAAAACCCTTTCCCGGG‑G‑AA‑CCCCAACGGGGaaaagaaa                                                                                  >  2:2511942/1‑136 (MQ=255)
                                                             aTCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGG‑TAAAAAAACGTGG‑GAAAACAAACAACTCCCTTTCCCGA‑G‑AA‑CCCCCACGGGGGAAAAGAAATTTaa                                                                            >  1:354746/1‑140 (MQ=255)
                                                             aTCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGG‑GAAAAAAAAGTGG‑CATAACAAAAAACCCCTTGTCCGGA‑G‑AA‑CCCCAACGGGGAAAAAGAAATTCaa                                                                            >  1:1898512/1‑140 (MQ=255)
                                                             aTCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGG‑GAAAAAAAAGTGG‑CATAAAAAAAAAACCCTTCTCCGGG‑G‑AA‑CCCCAACGGGGGAAAAGaaaattaa                                                                            >  2:1038737/1‑135 (MQ=255)
                                                             aTCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGG‑GAAAAAAAACGGG‑GATAAAAAACAAAACCTTTTCCGGA‑GAAA‑ACCCAAGGGGGAAAA‑GAAAATCaa                                                                            >  2:1435249/1‑140 (MQ=255)
                                                              tCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGG‑TAAAAAAACGTGG‑CATAACAAACAACTCCTTGTCCGGA‑G‑AA‑CCCCAACGGGGAAAACGAAATTCAAt                                                                           <  1:295637/140‑1 (MQ=255)
                                                              tCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGG‑TAAAAAAACGTGG‑CATAACAAACAACTCCTTGTCCGGA‑G‑AA‑CCCCAACGGGGAAAACGAAATTCAAt                                                                           <  2:2910269/140‑1 (MQ=255)
                                                               ccGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGG‑TAAAAAAACGTGG‑CATAACAAACAACTCCTTGTCCGGA‑G‑AA‑CCCCAACGGGGAAAACGAAATTCAATa                                                                          <  1:1498342/140‑1 (MQ=255)
                                                                 gTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGG‑TAAAAAAACGTGG‑CATAACAAACAACTCCTTGTCCGGA‑G‑AA‑CCCCAACGGGGAAAACGAAATTCAATAcc                                                                        <  1:1012463/140‑1 (MQ=255)
                                                                 gTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGG‑TAAAAAAACGTGG‑CATAACAAACAACTCCTTGTCCGGA‑G‑AA‑CCCCAACGGGGAAAACGAAATTCAATAcc                                                                        <  1:157189/140‑1 (MQ=255)
                                                                 gTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGG‑TAAAAAAACGTGG‑CATAACAAACAACTCCTTGTCCGGA‑G‑AA‑CCCCAACGGGGAAAACGAAATTCAATAcc                                                                        <  1:2511942/140‑1 (MQ=255)
                                                                 gTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGG‑GAAAAAAAAGTGG‑CATAACAAAAAACTCCTTGTCCGGA‑G‑AA‑CCCCAACGGGGGAAAAGAAATTCAATAcc                                                                        >  1:790485/1‑140 (MQ=255)
                                                                  tCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGG‑GAAAAAAAACCGG‑CATAAGAAACAACTCCTTGTCCCGG‑G‑AA‑ACCCAACGGGGGGAAGGAAAATCAAAAccc                                                                       >  2:584630/1‑139 (MQ=255)
                                                                     aCAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGG‑TAAAAAAACGTGG‑CATAACAAACAACTCCTTGTCCGGA‑G‑AA‑CCCCAACGGGGAAAACGAAATTCAATACCGTCg                                                                    <  2:586839/140‑1 (MQ=255)
                                                                       aGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGG‑TAAAAAAACGTGG‑CATAACAAACAACTCCTTGTCCGGA‑G‑AA‑CCCCAACGGGGAAAACGAAATTCAATACCGTCGGt                                                                  <  1:1954441/140‑1 (MQ=255)
                                                                       aGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGG‑GAAAAAAACACGG‑CATAAAAAAAAACTCCCTTTCCGGA‑G‑GA‑CCCCAACGGGGGAAACGAAAATCAATAcctgtctc                                                                  >  2:1359118/1‑134 (MQ=255)
                                                                        gATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGG‑TAAAAAAACGTGG‑CATAACAAACAACTCCTTGTCCGGA‑G‑AA‑CCCCAACGGGGAAAACGAAATTCAATACCGTCGGTa                                                                 <  1:2501642/140‑1 (MQ=255)
                                                                         aTACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGG‑TAAAAAAACGTGG‑CATAACAAACAACTCCTTGTCCGGA‑G‑AA‑CCCCAACGGGGAAAACGAAATTCAATACCGTCGGTaa                                                                <  1:1733186/140‑1 (MQ=255)
                                                                         aTACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGG‑TAAAAAAAAATGG‑GATAACAAACAACTCCTTGTCCGGA‑G‑GA‑CCCCAACGGGGGAAAAGAAAATCAATAACGCCGGGaa                                                                >  2:1092691/1‑140 (MQ=255)
                                                                         aTACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGG‑GAAAAAAAAGCGG‑CATAAAAAAAAAAACCTTGTCCGGA‑G‑AA‑CCCCAAAGGGGGAAAAGAAAATCAATACCCTCGGGaa                                                                >  2:157189/1‑140 (MQ=255)
                                                                         aTACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGG‑GAAAAAAAAGAGG‑CATAAAAAAAAACTCCTTGTCCGGA‑G‑AA‑CCCCAACGGGGGAAACGAAAATCAATACCGTCGGGaa                                                                >  2:42869/1‑140 (MQ=255)
                                                                              cagcaAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGG‑TAAAAAAACGTGG‑CATAACAAACAACTCCTTGTCCGGA‑G‑AA‑CCCCAACGGGGAAAACGAAATTCAATACCGTCGGTAATGACg                                                           <  1:966280/140‑1 (MQ=255)
                                                                               agcaAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGG‑TAAAAAAACGTGG‑CATAACAAACAACTCCTTGTCCGGA‑G‑AA‑CCCCAACGGGGAAAACGAAATTCAATACCGTCGGTAATGACGa                                                          <  1:1030473/140‑1 (MQ=255)
                                                                                 caAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGG‑TAAAAAAACGTGG‑CATAACAAACAACTCCTTGTCCGGA‑G‑AA‑CCCCAACGGGGAAAACGAAATTCAATACCGTCGGTAATGACGaaa                                                        <  2:80236/140‑1 (MQ=255)
                                                                                 caAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGG‑TAAAAAAACGTGG‑CATAACAAACAACTCCTTGTCCGGA‑G‑AA‑CCCCAACGGGGAAAACGAAATTCAATACCGTCGGTAATGACGaaa                                                        <  1:1541829/140‑1 (MQ=255)
                                                                                 caAAGTCATACAAAATAAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGG‑TAAAAAAACGTGG‑CATAACAAACAACTCCTTGTCCGGA‑G‑AA‑CCCCAACGGGGAAAACGAAATTCAATACCGTCGGTAATGACGaaa                                                        <  1:1985183/140‑1 (MQ=255)
                                                                                   aaGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGG‑TAAAAAAACGTGG‑CATAACAAACAACTCCTTGTCCGGA‑G‑AA‑CCCCAACGGGGAAAACGAAATTCAATACCGTCGGTAATGACGAAAgg                                                      <  1:630946/140‑1 (MQ=255)
                                                                                      tCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGG‑GAAAAAAAAGTGG‑CATAAAAAACAACTCCTTGTCCGGA‑G‑AA‑CCCCAACGGGGAAAACGAAAATCAATACCCTCGGGAATGACGAAAAGCAg                                                   >  2:1926035/1‑140 (MQ=255)
                                                                                           cAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGG‑TAAAAAAACGTGG‑CATAACAAACAACTCCTTGTCCGGA‑G‑AA‑CCCCAACGGGGAAAACGAAATTCAATACCGTCGGTAATGACGAAAGGCAGGTgag                                              <  1:1043357/140‑1 (MQ=255)
                                                                                              aaTCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGG‑TAAAAAAACGTGG‑CATAACAAACAACTCCTTGTCCGGA‑G‑AA‑CCCCAACGGGGAAAACGAAATTCAATACCGTCGGTAATGACGAAAGGCAGGTGAGAAg                                           <  2:342085/140‑1 (MQ=255)
                                                                                                   aCGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGG‑GAAAAAAAAGAGG‑CATAAAAAAAAACTCCTTGTCCGGA‑G‑AA‑CCCCAACGGGGGAAAAGAAAATCAAAACCCTCGGGAATGACGAAAAGGAGGGGAGAAGGGCaa                                      >  2:2498077/1‑140 (MQ=255)
                                                                                                    cGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGG‑GAAAAAAAAGGGG‑CATAAAAAACAAATCCTTGTCCGGG‑G‑AA‑CCCCCACGGGGGAAAAGAAAATCAATACCGTCGGGAATGACGAAAAGCAGGGGGGGAGGGGaaa                                     >  2:1217463/1‑139 (MQ=255)
                                                                                                         tCGGGAAAAACAGCGTGGCGGCATGGCGGG‑GAAAAAAACGTGG‑CATAACAAACAACTCCTTGTCCGGA‑G‑AA‑CCCCAACGGGGAAAAAGAAATTCAATACCGTCGGGAATGACGAAAGGCAGGGGAGAAGGGCAACGAGAc                                >  1:1618036/1‑140 (MQ=255)
                                                                                                              aaaaaCAGCGTGGCGGCATGGCGGG‑TAAAAAAACGTGG‑CATAACAAACAACTCCTTGTCCGGA‑G‑AA‑CCCCAACGGGGAAAACGAAATTCAATACCGTCGGTAATGACGAAAGGCAGGTGAGAAGGGCAACGAGACTGCaa                           <  2:411/140‑1 (MQ=255)
                                                                                                              aaaaaCAGCGTGGCGGCATGGCGGG‑TAAAAAAACGTGG‑CATAACAAACAACTCCTTGTCCGGA‑G‑AA‑CCCCAACGGGGAAAACGAAATTCAATACCGTCGGTAATGACGAAAGGCAGGTGAGAAGGGCAACGAGACTGCaa                           <  2:1898512/140‑1 (MQ=255)
                                                                                                                           cggcATGGCGGG‑GTAAAAAACACGG‑CGCAAAAAACAACTCCCTGTCCGGG‑G‑AA‑CCCCAACGGGGAAAACGAAATTCAATACCGTCGGGAAAGACGAAAAGGAAGGGAGAAAGGGAAAGAGACTGCAAATGTACTGAAatt              >  2:2473654/1‑140 (MQ=255)
                                                                                                                               aTGGCGGG‑TAAAAAAACGTGG‑CATAACAAACAACTCCTTGTCCGGA‑G‑AA‑CCCCAACGGGGAAAACGAAATTCAATACCGTCGGTAATGACGAAAGGCAGGTGAGAAGGGCAACGAGACTGCAAATGTACTGAATTTCATg          <  1:1217463/140‑1 (MQ=255)
                                                                                                                                 ggCGGG‑TAAAAAAACGTGG‑CATAACAAACAACTCCTTGTCCGGA‑G‑AA‑CCCCAACGGGGAAAACGAAATTCAATACCGTCGGTAATGACGAAAGGCAGGTGAGAAGGGCAACGAGACTGCAAATGTACTGAATTTCATGTa        <  2:1485115/140‑1 (MQ=255)
                                                                                                                                   cGGG‑TAAAAAAACGTGG‑CATAACAAACAACTCCTTGTCCGGA‑G‑AA‑CCCCAACGGGGAAAACGAAATTCAATACCGTCGGTAATGACGAAAGGCAGGTGAGAAGGGCAACGAGACTGCAAATGTACTGAATTTCATGTAAt      <  1:2756475/140‑1 (MQ=255)
                                                                                                                                        tAAAAAAACGTGG‑CATAACAAACAACTCCTTGTCCGGA‑G‑AA‑CCCCAACGGGGAAAACGAAATTCAATTCCGTCGGTAATGACGAAAGGCAGGTGAGAAGGGCAACGAGACTGCAAATGTACTGAATTTCATGTAATCAAc  <  1:584630/140‑1 (MQ=255)
                                                                                                                                        tAAAAAAACGTGG‑CATAACAAACAACTCCTTGTCCGGA‑G‑AA‑CCCCAACGGGGAAAACGAAATTCAATACCGTCGGTAATGACGAAAGGCAGGTGAGAAGGGCAACGAGACTGCAAATGTACTGAATTTCATGTAATCAAc  <  1:42869/140‑1 (MQ=255)
                                                                                                                                        |                                                                                                                                               
GGGCCAGACTGACATCGGCATTAAAATCACGTACCACATTAATGATCCCAGGCTGGATCAGATCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGG‑TAAAAAAACGTGG‑CATAACAAACAACTCCTTGTCCGGA‑G‑AA‑CCCCAACGGGGAAAACGAAATTCAATACCGTCGGTAATGACGAAAGGCAGGTGAGAAGGGCAACGAGACTGCAAATGTACTGAATTTCATGTAATCAAC  >  NC_000913/4568369‑4568643

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑