BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,045,723	C→A	Q27H (CAG→CAT)	gfcE ←	putative exopolysaccharide export lipoprotein GfcE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,045,723	0	C	A	100.0%	786.5 / NA	218	Q27H (CAG→CAT)	gfcE	putative exopolysaccharide export lipoprotein GfcE
Reads supporting (aligned to +/- strand): ref base C (0/0); new base A (101/117); total (101/117)

Only 100 of 221 total aligned reads displayed.

TAGCCTGCGACATAATCGGTTTGGCGCGCAACTGATCAATCAATCCTGGCGTGACGGGATAGATATTGACCATTTTGTCCAGGTCATACTGATTGTCAGGTAGTTCAATGACATCCTTGTTACTGGTCGATAAATTCTGACCAGGAACAAGGGTACATGCTGTCAAAGACAGAACAGCCAATGTCAGTACCGAAAACTTAAAAATGTTCTTTTTCATTGGTTTATATTTATCAGTCAATGTAATGTTATGAATTACGTCAGGTTATTGTCCA  >  NC_000913/1045587‑1045858

tAGCCTGCGACATAATCGGTTTGGCGCGCAACTGATCAATCAATCCTGGCGTGACGGGATAGATATTGACCATTTTGTCCAGGTCATACTGATTGTCAGGTAGTTCAATGACATCCTTGTTACTGGTCGATAAATTATGa                                                                                                                                      <  1:1160846/140‑1 (MQ=255)

  gCCTGCGACATAATCGGTTTGGCGCGCAACTGATCAATCAATCCTGGCGTGACGGGATAGATATTGACCATTTTGTCCAGGTCATACTGATTGTCAGGTAGTTCAATGACATCCTTGTTACTGGTCGATAAATTATGAcc                                                                                                                                    >  1:877557/1‑140 (MQ=255)

  gCCTGCGACATAATCGGTTTGGCGCGCAACTGATCAATCAATCCTGGCGTGACGGGATAGATATTGACCATTTTGTCCAGGTCATACTGATTGTCAGGTAGTTCAATGACATCCTTGTTACTGGTCGATAAATTATGAcc                                                                                                                                    <  1:317273/140‑1 (MQ=255)

  gCCTGCGACATAATCGGTTTGGCGCGCAACTGATCAATCAATCCTGGCGTGACGGGATAGATATTGACCATTTTGTCCAGGTCATACTGATTGTCAGGTAGTTCAATGACATCCTTGTTACTGGTCGATAAATTATGAcc                                                                                                                                    <  1:2792997/140‑1 (MQ=255)

  gCCTGCGACATAATCGGTTTGGCGCGCAACTGATCAATCAATCCTGGCGTGACGGGATAGATATTGACCATTTTGTCCAGGTCATACTGATTGTCAGGTAGTTCAATGACATCCTTGTTACTGGTCGATAAATTATGAcc                                                                                                                                    <  2:237958/140‑1 (MQ=255)

  gCCTGCGACATAATCGGTTTGGCGCGCAACTGATCAATCAATCCTGGCGTGACGGGATAGATATTGACCATTTTGTCCAGGTCATACTGATTGTCAGGTAGTTCAATGACATCCTTGTTACTGGTCGATAAATTATGAcc                                                                                                                                    <  2:2405789/140‑1 (MQ=255)

  gCCTGCGACATAATCGGTTTGGCGCGCAACTGATCAATCAATCCTGGCGTGACGGGATAGATATTGACCATTTTGTCCAGGTCATACTGATTGTCAGGTAGTTCAATGACATCCTTGTTACTGGTCGATAAATTATGAcc                                                                                                                                    >  2:2647353/1‑140 (MQ=255)

  gCCTGCGACATAATCGGTTTGGCGCGCAACTGATCAATCAATCCTGGCGTGACGGGATAGATATTGACCATTTTGTCCAGGTCATACTGATTGTCAGGTAGTTCAATGACATCCTTGTTACTGGTCGATAAATTATGAcc                                                                                                                                    <  1:1701515/140‑1 (MQ=255)

     tGCGACATAATCGGTTTGGCGCGCAACTGATCAATCAATCCTGGCGTGACGGGATAGATATTGACCATTTTGTCCAGGTCATACTGATTGTCAGGTAGTTCAATGACATCCTTGTTACTGGTCGATAAATTATGACCAgg                                                                                                                                 <  1:1858247/140‑1 (MQ=255)

       cGACATAATCGGTTTGGCGCGCAACTGATCAATCAATCCTGGCGTGACGGGATAGATATTGACCATTTTGTCCAGGTCATACTGATTGTCAGGTAGTTCAATGACATCCTTGTTACTGGTCGATAAATTATGACCAGGaa                                                                                                                               >  2:1079659/1‑140 (MQ=255)

        gACATAATCGGTTTGGCGCGCAACTGATCAATCAATCCTGGCGTGACGGGATAGATATTGACCATTTTGTCCAGGTCATACTGATTGTCAGGTAGTTCAATGACATCCTTGTTACTGGTCGATAAATTATGACCAGGAAc                                                                                                                              >  2:1086111/1‑140 (MQ=255)

        gACATAATCGGTTTGGCGCGCAACTGATCAATCAATCCTGGCGTGACGGGATAGATATTGACCATTTTGTCCAGGTCATACTGATTGTCAGGTAGTTCAATGACATCCTTGTTACTGGTCGATAAATTATGACCAGGAAc                                                                                                                              <  2:1911636/140‑1 (MQ=255)

         aCATAATCGGTTTGGCGCGCAACTGATCAATCAATCCTGGCGTGACGGGATAGATATTGACCATTTTGTCCAGGTCATACTGATTGTCAGGTAGTTCAATGACATCCTTGTTACTGGTCGATAAATTATGACCAGGAACa                                                                                                                             <  2:548193/140‑1 (MQ=255)

              aTCGGTTTGGCGCGCAACTGATCAATCAATCCTGGCGTGACGGGATAGATATTGACCATTTTGTCCAGGTCATACTGATTGTCAGGTAGTTCAATGACATCCTTGTTACTGGTCGATAAATTATGACCAGGAACAAGGGt                                                                                                                        <  2:2268859/140‑1 (MQ=255)

                    ttGGCGCGCAACTGATCAATCAATCCTGGCGTGACGGGATAGATATTGACTATTTTGTCCAGGTCATACTGATTGTCAGGTAGTTCAATGACATCCTTGTTACTGGTCGATAAATTATGACCAGGAACAAGGGTACATGc                                                                                                                  >  1:380238/1‑140 (MQ=255)

                    ttGGCGCGCAACTGATCAATCAATCCTGGCGTGACGGGATAGATATTGACCATTTTGTCCAGGTCATACTGATTGTCAGGTAGTTCAATGACATCCTTGTTACTGGTCGATAAATTATGAcc                                                                                                                                    >  1:1774618/1‑122 (MQ=255)

                         gcgcAACTGATCAATCAATCCTGGCGTGACGGGATAGATATTGACCATTTTGTCCAGGTCATACTGATTGTCAGGTAGTTCAATGACATCCTTGTTACTGGTCGATAAATTATGACCAGGAACAAGGGTACATGCTGTCa                                                                                                             >  2:2006889/1‑140 (MQ=255)

                             aaCTGATCAATCAATCCTGGCGTGACGGGATAGATATTGACCATTTTGTCCAGGTCATACTGATTGTCAGGTAGTTCAATGACATCCTTGTTACTGGTCGATAAATTATGACCAGGAACAAGGGTACATGCTGTCAAAGa                                                                                                         >  1:1292406/1‑140 (MQ=255)

                                  attgatcaatcCTGGCGTGACGGGATAGATATTGACCATTTTGTCCAGGTCATACTGATTGTCAGGTAGTTCAATGACATCCTTGTTACTGGTCGATAAATTATGACCAGGAACAAGGGTACATGCTGTCAAAGACAGaa                                                                                                    <  2:2472857/136‑1 (MQ=255)

                                  atcaatcaATCCTGGCGTGACGGGATAGATATTGACCATTTTGTCCAGGTCATACTGATTGTCAGGTAGTTCAATGACATCCTTGTTACTGGTCGATAAATTATGACCAGGAACAAGGGTACATGCTGTCAAAGACAGaa                                                                                                    <  2:1142966/140‑1 (MQ=255)

                                       tcaatcCTGGCGTGACGGGATAGATATTGACCATTTTGTCCAGGTCATACTGATTGTCAGGTAGTTCAATGACATCCTTGTTACTGGTCGATAAATTATGACCAGGAACAAGGGTACATGCTGTCAAAGACAGAACAGcc                                                                                               <  1:728345/140‑1 (MQ=255)

                                       tcaatcCTGGCGTGACGGGATAGATATTGACCATTTTGTCCAGGTCATACTGATTGTCAGGTAGTTCAATGACATCCTTGTTACTGGTCGATAAATTATGACCAGGAACAAGGGTACATGCTGTCAAAGACAGAACAGcc                                                                                               <  1:2529480/140‑1 (MQ=255)

                                       tcaatcCTGGCGTGACGGGATAGATATTGACCATTTTGTCCAGGTCATACTGATTGTCAGGTAGTTCAATGACATCCTTGTTACTGGTCGATAAATTATGACCAGGAACAAGGGTACATGCTGTCAAAGACAGAACAGcc                                                                                               <  2:2189906/140‑1 (MQ=255)

                                       tcaatcCTGGCGTGACGGGATAGATATTGACCATTTTGTCCAGGTCATACTGATTGTCAGGTAGTTCAATGACATCCTTGTTACTGGTCGATAAATTATGACCAGGAACAAGGGTACATGCTGTCAAAGACAGAACAGcc                                                                                               >  2:2830062/1‑140 (MQ=255)

                                            ccTGGCGTGACGGGATAGATATTGACCATTTTGTCCAGGTCATACTGATTGTCAGGTAGTTCAATGACATCCTTGTTACTGGTCGATAAATTATGACCAGGAACAAGGGTACATGCTGTCAAAGACAGAACAGCCAATGt                                                                                          >  2:2376129/1‑140 (MQ=255)

                                              tGGCGTGACGGGATAGATATTGACCATTTTGTCCAGGTCATACTGATTGTCAGGTAGTTCAATGACATCCTTGTTACTGGTCGATAAATTATGACCAGGAACAAGGGTACATGCTGTCAAAGACAGAACAGCCAATGTCa                                                                                        >  2:1528664/1‑140 (MQ=255)

                                                gCGTGACGGGATAGATATTGACCATTTTGTCCAGGTCATACTGATTGTCAGGTAGTTCAATGACATCCTTGTTACTGGTCGATAAATTATGACCAGGAACAAGGGTACATGCTGTCAAAGACAGAACAGCCAATGTCAGt                                                                                      >  1:138898/1‑140 (MQ=255)

                                                 cGTGACGGGATAGATATTGACCATTTTGTCCAGGTCATACTGATTGTCAGGTAGTTCAATGACATCCTTGTTACTGGTCGATAAATTATGACCAGGAACAAGGGTACATGCTGTCAAAGACAGAACAGCCAATGTCAGTa                                                                                     >  2:1754504/1‑140 (MQ=255)

                                                     aCGGGATAGATATTGACCATTTTGTCCAGGTCATACTGATTGTCAGGTAGTTCAATGACATCCTTGTTACTGGTCGATAAATTATGACCAGGAACAAGGGTACATGCTGTCAAAGACAGAACAGCCAATGTCAGTACCGa                                                                                 >  2:2755263/1‑140 (MQ=255)

                                                     aCGGGATAGATATTGACCATTTTGTCCAGGTCATACTGATTGTCAGGTAGTTCAATGACATCCTTGTTACTGGTCGATAAATTATGACCAGGAACAAGGGTACATGCTGTCAAAGACAGAACAGCCAATGTCAGTACCGa                                                                                 >  2:1822310/1‑140 (MQ=255)

                                                     aCGGGATAGATATTGACCATTTTGTCCAGGTCATACTGATTGTCAGGTAGTTCAATGACATCCTTGTTACTGGTCGATAAATTATGACCAGGAACAAGGGTACATGCTGTCAAAGACAGAACAGCCAATGTCAGTACCGa                                                                                 >  1:479601/1‑140 (MQ=255)

                                                     aCGGGATAGATATTGACCATTTTGTCCAGGTCATACTGATTGTCAGGTAGTTCAATGACATCCTTGTTACTGGTCGATAAATTATGACCAGGAACAAGGGTACATGCTGTCAAAGACAGAACAGCCAATGTCAGTACCGa                                                                                 >  2:2038809/1‑140 (MQ=255)

                                                     aCGGGATAGATATTGACCATTTTGTCCAGGTCATACTGATTGTCAGGTAGTTCAATGACATCCTTGTTACTGGTCGATAAATTATGACCAGGAACAAGGGTACATGCTGTCAAAGACAGAACAGCCAATGTCAGTACCGa                                                                                 >  1:1437867/1‑140 (MQ=255)

                                                     aCGGGATAGATATTGACCATTTTGTCCAGGTCATACTGATTGTCAGGTAGTTCAATGACATCCTTGTTACTGGTCGATAAATTATGACCAGGAACAAGGGTACATGCTGTCAAAGACAGAACAGCCAATGTCAGTACCGa                                                                                 >  1:955004/1‑140 (MQ=255)

                                                       gggATAGATATTGACCATTTTGTCCAGGTCATACTGATTGTCAGGTAGTTCAATGACATCCTTGTTACTGGTCGATAAATTATGACCAGGAACAAGGGTACATGCTGTCAAAGACAGAACAGCCAATGTCAGTACCGaaa                                                                               <  1:1541826/140‑1 (MQ=255)

                                                             gataTTGACCATTTTGTCCAGGTCATACTGATTGTCAGGTAGTTCAATGACATCCTTGTTACTGGTCGATAAATTATGACCAGGAACAAGGGTACATGCTGTCAAAGACAGAACAGCCAATGTCAGTACCGa                                                                                 >  1:518638/1‑132 (MQ=255)

                                                                   tacccctttGTCCAGGGCCTTCTGATTGTCAGGAAGTTCAAGCACATCGTTGTGACGGATCGTTAAATTATGATCAGCAACAAGGGGACATGCTGTCAAAGACAGAACAGCCAATATCAGTACCGAAAACTTAAAAATGt                                                                   >  2:2579209/7‑140 (MQ=255)

                                                                   gACCATTTTGTCCAGGTCATACTGATTGTCAGGTAGTTCAATGACATCCTTGTTACTGGTCGATAAATTATGACCAGGAACAAGGGTACATGCTGTCAAAGACAGAACAGCCAATGTCAGTACCGAAAACTTAAAAATGt                                                                   >  1:2909937/1‑140 (MQ=255)

                                                                   gACCATTTTGTCCAGGTCATACTGATTGTCAGGTAGTTCAATGACATCCTTGTTACTGGTCGATAAATTATGACCAGGAACAAGGGTACATGCTGTCAAAGACAGAACAGCCAATGTCAGTACCGAAAACTTAAAAATGt                                                                   >  1:2231783/1‑140 (MQ=255)

                                                                   gACCATTTTGTCCAGGTCATACTGATTGTCAGGTAGTTCAATGACATCCTTGTTACTGGTCGATAAATTATGACCAGGAACAAGGGTACATGCTGTCAAAGACAGAACAGCCAATGTCAGTACCGAAAACTTAAAAATGt                                                                   >  2:680157/1‑140 (MQ=255)

                                                                    aCCATTTTGTCCAGGTCATACTGATTGTCAGGTAGTTCAATGACATCCTTGTTACTGGTCGATAAATTATGACCAGGAACAAGGGTACATGCTGTCAAAGACAGAACAGCCAATGTCAGTACCGAAAACTTAAAAATGtt                                                                  <  2:380238/140‑1 (MQ=255)

                                                                         tttGTCCAGGTCATACTGATTGTCAGGTAGTTCAATGACATCCTTGTTACTGGTCGATAAATTATGACCAGGAACAAGGGTACATGCTGTCAAAGACAGAACAGCCAATGTCAGTACCGAAAACTTAAAAATGTTCtttt                                                             >  2:2692243/1‑140 (MQ=255)

                                                                         tttGTCCAGGTCATACTGATTGTCAGGTAGTTCAATGACATCCTTGTTACTGGTCGATAAATTATGACCAGGAACAAGGGTACATGCTGTCAAAGACAGAACAGCCAATGTCAGTACCGAAAACTTAAAAATGTTCtttt                                                             >  1:106942/1‑140 (MQ=255)

                                                                            gTCCAGGTCATACTGATTGTCAGGTAGTTCAATGACATCCTTGTTACTGGTCGATAAATTATGACCAGGAACAAGGGTACATGCTGTCAAAGACAGAACAGCCAATGTCAGTACCGAAAACTTAAAAATGTTCTTTTTCa                                                          <  2:877557/140‑1 (MQ=255)

                                                                            gTCCAGGTCATACTGATTGTCAGGTAGTTCAATGACATCCTTGTTACTGGTCGATAAATTATGACCAGGAACAAGGGTACATGCTGTCAAAGACAGAACAGCCAATGTCAGTACCGAAAACTTAAAAATGTTCTTTTTCa                                                          <  2:2115034/140‑1 (MQ=255)

                                                                            gTCCAGGTCATACTGATTGTCAGGTAGTTCAATGACATCCTTGTTACTGGTCGATAAATTATGACCAGGAACAAGGGTACATGCTGTCAAAGACAGAACAGCCAATGTCAGTACCGAAAACTTAAAAATGTTCTTTTTCa                                                          <  1:1211196/140‑1 (MQ=255)

                                                                            gTCCAGGTCATACTGATTGTCAGGTAGTTCAATGACATCCTTGTTACTGGTCGATAAATTATGACCAGGAACAAGGGTACATGCTGTCAAAGACAGAACAGCCAATGTCAGTACCGAAAACTTAAAAATGTTCTTTTTCa                                                          <  2:785424/140‑1 (MQ=255)

                                                                                  gTCATACTGATTGTCAGGTAGTTCAATGACATCCTTGTTACTGGTCGATAAATTATGACCAGGAACAAGGGTACATGCTGTCAAAGACAGAACAGCCAATGTCAGTACCGAAAACTTAAAAATGTTCTTTTTCATTGGtt                                                    >  1:2764476/1‑140 (MQ=255)

                                                                                   tCATACTGATTGTCAGGTAGTTCAATGACATCCTTGTTACTGGTCGATAAATTATGACCAGGAACAAGGGTACATGCTGTCAAAGACAGAACAGCCAATGTCAGTACCGAAAACTTAAAAATGTTCTTTTTCATTGGttt                                                   <  1:1084134/140‑1 (MQ=255)

                                                                                       aCTGATTGTCAGGTAGTTCAATGACATCCTTGTTACTGGTCGATAAATTATGACCAGGAACAAGGGTACATGCTGTCAAAGACAGAACAGCCAATGTCAGTACCGAAAACTTAAAAATGTTCTTTTTCATTGGTTTatat                                               >  2:699360/1‑140 (MQ=255)

                                                                                       aCTGATTGTCAGGTAGTTCAATGACATCCTTGTTACTGGTCGATAAATTATGACCAGGAACAAGGGTACATGCTGTCAAAGACAGAACAGCCAATGTCAGTACCGAAAACTTAAAAATGTTCTTTTTCATTGGTTTatat                                               >  2:593739/1‑140 (MQ=255)

                                                                                       aCTGATTGTCAGGTAGTTCAATGACATCCTTGTTACTGGTCGATAAATTATGACCAGGAACAAGGGTACATGCTGTCAAAGACAGAACAGCCAATGTCAGTACCGAAAACTTAAAAATGTTCTTTTTCATTGGTTTatat                                               >  1:2249811/1‑140 (MQ=255)

                                                                                          gATTGTCAGGTAGTTCAATGACATCCTTGTTACTGGTCGATAAATTATGACCAGGAACAAGGGTACATGCTGTCAAAGACAGAACAGTCAATGTCAGTACCGAAAACTTAAAAATGTTCTTTTTCATTGGTTTATATTTa                                            <  2:1292406/140‑1 (MQ=255)

                                                                                          gATTGTCAGGTAGTTCAATGACATCCTTGTTACTGGTCGATAAATTATGACCAGGAACAAGGGTACATGCTGTCAAAGACAGAACAGCCAATGTCAGTACCGAAAACTTAAAAATGTTCTTTTTCATTGGTTTATATTTa                                            <  2:479601/140‑1 (MQ=255)

                                                                                          gATTGTCAGGTAGTTCAATGACATCCTTGTTACTGGTCGATAAATTATGACCAGGAACAAGGGTACATGCTGTCAAAGACAGAACAGCCAATGTCAGTACCGAAAACTTAAAAATGTTCTTTTTCATTGGTTTATATTTa                                            <  1:2466237/140‑1 (MQ=255)

                                                                                          gATTGTCAGGTAGTTCAATGACATCCTTGTTACTGGTCGATAAATTATGACCAGGAACAAGGGTACATGCTGTCAAAGACAGAACAGCCAATGTCAGTACCGAAAACTTAAAAATGTTCTTTTTCATTGGTTTATATTTa                                            <  1:1754504/140‑1 (MQ=255)

                                                                                          gATTGTCAGGTAGTTCAATGACATCCTTGTTACTGGTCGATAAATTATGACCAGGAACAAGGGTACATGCTGTCAAAGACAGAACAGCCAATGTCAGTACCGAAAACTTAAAAATGTTCTTTTTCATTGGTTTATATTTa                                            <  1:573027/140‑1 (MQ=255)

                                                                                           aTTGTCAGGTAGTTCAATGACATCCTTGTTACTGGTCGATAAATTATGACCAGGAACAAGGGTACATGCTGTCAAAGACAGAACAGCCAATGTCAGTACCGAAAACTTAAAAATGTTCTTTTTCATTGGTTTATATTTAt                                           >  1:2377572/1‑140 (MQ=255)

                                                                                           aTTGTCAGGTAGTTCAATGACATCCTTGTTACTGGTCGATAAATTATGACCAGGAACAAGGGTACATGCTGTCAAAGACAGAACAGCCAATGTCAGTACCGAAAACTTAAAAATGTTCTTTTTCATTGGTTTATATTTAt                                           <  2:1950013/140‑1 (MQ=255)

                                                                                            ttGTCAGGTAGTTCAATGACATCCTTGTTACTGGTCGATAAATTATGACCAGGAACAAGGGTACATGCTGTCAAAGACAGAACAGCCAATGTCAGTACCGAAAACTTAAAAATGTTCTTTTTCATTGGTTTATATTTATc                                          <  1:2699868/140‑1 (MQ=255)

                                                                                              gTCAGGTAGTTCAATGACATCCTTGTTACTGGTCGATAAATTATGACCAGGAACAAGGGTACATGCTGTCAAAGACAGAACAGCCAATGTCAGTACCGAAAACTTAAAAATGTTCTTTTTCATTGGTTTATATTTATCAg                                        <  2:1437867/140‑1 (MQ=255)

                                                                                              gTCAGGTAGTTCAATGACATCCTTGTTACTGGTCGATAAATTATGACCAGGAACAAGGGTACATGCTGTCAAAGACAGAACAGCCAATGTCAGTACCGAAAACTTAAAAATGTTCTTTTTCATTGGTTTATATTTATCAg                                        <  1:2869335/140‑1 (MQ=255)

                                                                                              gTCAGGTAGTTCAATGACATCCTTGTTACTGGTCGATAAATTATGACCAGGAACAAGGGTACATGCTGTCAAAGACAGAACAGCCAATGTCAGTACCGAAAACTTAAAAATGTTCTTTTTCATTGGTTTATATTTATCAg                                        <  2:364631/140‑1 (MQ=255)

                                                                                               tCAGGTAGTTCAATGACATCCTTGTTACTGGTCGATAAATTATGACCAGGAACAAGGGTACATGCTGTCAAAGACAGAACAGCCAATGTCAGTACCGAAAACTTAAAAATGTTCTTTTTCATTGGTTTATATTTATCAGt                                       <  1:2038809/140‑1 (MQ=255)

                                                                                               tCAGGTAGTTCAATGACATCCTTGTTACTGGTCGATAAATTATGACCAGGAACAAGGGTACATGCTGTCAAAGACAGAACAGCCAATGTCAGTACCGAAAACTTAAAAATGTTCTTTTTCATTGGTTTATATTTATCAGt                                       <  2:514557/140‑1 (MQ=255)

                                                                                               tCAGGTAGTTCAATGACATCCTTGTTACTGGTCGATAAATTATGACCAGGAACAAGGGTACATGCTGTCAAAGACAGAACAGCCAATGTCAGTACCGAAAACTTAAAAATGTTCTTTTTCATTGGTTTATATTTATCAGt                                       <  2:31481/140‑1 (MQ=255)

                                                                                               tCAGGTAGTTCAATGACATCCTTGTTACTGGTCGATAAATTATGACCAGGAACAAGGGTACATGCTGTCAAAGACAGAACAGCCAATGTCAGTACCGAAAACTTAAAAATGTTCTTTTTCATTGGTTTATATTTATCAGt                                       <  1:2745773/140‑1 (MQ=255)

                                                                                               tCAGGTAGTTCAATGACATCCTTGTTACTGGTCGATAAATTATGACCAGGAACAAGGGTACATGCTGTCAAAGACAGAACAGCCAATGTCAGTACCGAAAACTTAAAAATGTTCTTTTTCATTGGTTTATATTTATCAGt                                       <  1:2830062/140‑1 (MQ=255)

                                                                                               tCAGGTAGTTCAATGACATCCTTGTTACTGGTCGATAAATTATGACCAGGAACAAGGGTACATGCTGTCAAAGACAGAACAGCCAATGTCAGTACCGAAAACTTAAAAATGTTCTTTTTCATTGGTTTATATTTATCAGt                                       <  2:1967392/140‑1 (MQ=255)

                                                                                               tCAGGTAGTTCAATGACATCCTTGTTACTGGTCGATAAATTATGACCAGGAACAAGGGTACATGCTGTCAAAGACAGAACAGCCAATGTCAGTACCGAAAACTTAAAAATGTTCTTTTTCATTGGTTTATATTTATCAGt                                       <  1:593739/140‑1 (MQ=255)

                                                                                               tCAGGTAGTTCAATGACATCCTTGTTACTGGTCGATAAATTATGACCAGGAACAAGGGTACATGCTGTCAAAGACAGAACAGCCAATGTCAGTACCGAAAACTTAAAAATGTTCTTTTTCATTGGTTTATATTTATCAGt                                       <  2:1672106/140‑1 (MQ=255)

                                                                                                cAGGTAGTTCAATGACATCCTTGTTACTGGTCGATAAATTATGACCAGGAACAAGGGTACATGCTGTCAAAGACAGAACAGCCAATGTCAGTACCGAAAACTTAAAAATGTTCTTTTTCATTGGt                                                     >  1:220349/1‑125 (MQ=255)

                                                                                                cAGGTAGTTCAATGACATCCTTGTTACTGGTCGATAAATTATGACCAGGAACAAGGGTACATGCTGTCAAAGACAGAACAGCCAATGTCAGTACCGAAAACTTAAAAATGTTCTTTTTCATTGGTTTATATTTATCAGTc                                      >  2:77133/1‑140 (MQ=255)

                                                                                                    tAGTTCAATGACATCCTTGTTACTGGTCGATAAATTATGACCAGGAACAAGGGTACATGCTGTCAAAGACAGAACAGCCAATGTCAGTACCGAAAACTTAAAAATGTTCTTTTTCATTGGTTTATATTTATCAGTCaatg                                  <  1:184444/140‑1 (MQ=255)

                                                                                                     aGTTCAATGACATCCTTGTTACTGGTCGATAAATTATGACCAGGAACAAGGGTACATGCTGTCAAAGACAGAACAGCCAATGTCAGTACCGAAAACTTAAAAATGTTCTTTTTCATTGGTTTATATTTATCAGt                                       <  1:68832/134‑1 (MQ=255)

                                                                                                      gTTCAATGACATCCTTGTTACTGGTCGATAAATTATGACCGGGAACAAGGGTACATGCTGTCAAAGACAGAACAGCCAATGTCAGTACCGAAAACTTAAAAATGTTCTTTTTCATTGGTTTATATTTATCAGTCaatgta                                <  1:2489567/140‑1 (MQ=255)

                                                                                                      gTTCAATGACATCCTTGTTACTGGTCGATAAATTATGACCAGGAACAAGGGTACATGCTGTCAAAGACAGAACAGCCAATGTCAGTACCGAAAACTTAAAAATGTTCTTTTTCATTGGTTTATATTTATCAGTCaatgta                                <  1:418264/140‑1 (MQ=255)

                                                                                                      gTTCAATGACATCCTTGTTACTGGTCGATAAATTATGACCAGGAACAAGGGTACATGCTGTCAAAGACAGAACAGCCAATGTCAGTACCGAAAACTTAAAAATGTTCTTTTTCATTGGTTTATATTTATCAGTCaatgta                                <  1:1091768/140‑1 (MQ=255)

                                                                                                       ttCAATGACATCCTTGTTACTGGTCGATAAATTATGACCAGGAACAAGGGTACATGCTGTCAAAGACAGAACAGCCAATGTCAGTACCGAAAACTTAAAAATGTTCTTTTTCATTGGTTTATATTTATCAGTCaatgtaa                               >  2:2136929/1‑140 (MQ=255)

                                                                                                       ttCAATGACATCCTTGTTACTGGTCGATAAATTATGACCAGGAACAAGGGTACATGCTGTCAAAGACAGAACAGCCAATGTCAGTACCGAAAACTTAAAAATGTTCTTTTTCATTGGTTTATATTTATCAGTCaatgtaa                               >  2:527020/1‑140 (MQ=255)

                                                                                                         cAATGACATCCTTGTTACTGGTCGATAAATTATGACCAGGAACAAGGGTACATGCTGTCAAAGACAGAACAGCCAATGTCAGTACCGAAAACTTAAAAATGTTCTTTTTCATTGGTTTATATTTATCAGTCaatgtaatg                             >  2:1627067/1‑140 (MQ=255)

                                                                                                                 tCCTTGTTACTGGTCGATAAATTATGACCAGGAACAAGGGTACATGCTGTTAAAGACAGAACAGCCAATGTCAGTACCGAAAACTTAAAAATGTTCTTTTTCATTGGTTTATATTTATCAGt                                       >  1:2320528/1‑122 (MQ=255)

                                                                                                                 tCCTTGTTACTGGTCGATAAATTATGACCAGGAACAAGGGTACATGCTGTCAAAGACAGAACAGCCAATGTCAGTACCGAAAACTTAAAAATGTTCTTTTTCATTGGTTTATATTTATCAGt                                       <  1:930147/122‑1 (MQ=255)

                                                                                                                 tCCTTGTTACTGGTCGATAAATTATGACCAGGAACAAGGGTACATGCTGTCAAAGACAGAACAGCCAATGTCAGTACCGAAAACTTAAAAATGTTCTTTTTCATTGGTTTATATTTATCAGt                                       >  2:930147/1‑122 (MQ=255)

                                                                                                                 tCCTTGTTACTGGTCGATAAATTATGACCAGGAACAAGGGTACATGCTGTCAAAGACAGAACAGCCAATGTCAGTACCGAAAACTTAAAAATGTTCTTTTTCATTGGTTTATATTTATCAGTCAATGTAATGTTATGAAt                     <  2:2231783/140‑1 (MQ=255)

                                                                                                                 tCCTTGTTACTGGTCGATAAATTATGACCAGGAACAAGGGTACATGCTGTCAAAGACAGAACAGCCAATGTCAGTACCGAAAACTTAAAAATGTTCTTTTTCATTGGTTTATATTTATCAGTCAATGTAATGTTATGAAt                     >  2:725045/1‑140 (MQ=255)

                                                                                                                 tCCTTGTTACTGGTCGATAAATTATGACCAGGAACAAGGGTACATGCTGTCAAAGACAGAACAGCCAATGTCAGTACCGAAAACTTAAAAATGTTCTTTTTCATTGGTTTATATTTATCAGTCAATGTAATGTTATGAAt                     <  1:140690/140‑1 (MQ=255)

                                                                                                                      gTTACTGGTCGATAAATTATGACCAGGAACAAGGGTACATGCTGTCAAAGACAGAACAGCCAATGTCAGTACCGAAAACTTAAAAATGTTCTTTTTCATTGGTTTATATTTATCAGTCAATGTAATGtt                           >  2:615913/1‑129 (MQ=255)

                                                                                                                      gTTACTGGTCGATAAATTATGACCAGGAACAAGGGTACATGCTGTCAAAGACAGAACAGCCAATGTCAGTACCGAAAACTTAAAAATGTTCTTTTTCATTGGTTTATATTTATCAGTCAATGTAATGtt                           <  1:615913/129‑1 (MQ=255)

                                                                                                                      gTTACTGGTCGATAAATTATGACCAGGAACAAGGGTACATGCTGTCAAAGACAGAACAGCCAATGTCAGTACCGAAAACTTAAAAATGTTCTTTTTCATTGGTTTATATTTATCAGTCAATGTAATGTTATGAATTACGt                >  2:2100280/1‑140 (MQ=255)

                                                                                                                        tACTGGTCGATAAATTATGACCAGGAACAAGGGTACATGCTGTCAAAGACAGAACAGCCAATGTCAGTACCGAAAACTTAAAAATGTTCTTTTTCATTGGTTTATATTTATCAGTCAATGTAATGTTATGAATTACGTCa              <  2:2892442/140‑1 (MQ=255)

                                                                                                                        tACTGGTCGATAAATTATGACCAGGAACAAGGGTACATGCTGTCAAAGACAGAACAGCCAATGTCAGTACCGAAAACTTAAAAATGTTCTTTTTCATTGGTTTATATTTATCAGTCAATGTAATGTTATGAATTACGTCa              <  1:725045/140‑1 (MQ=255)

                                                                                                                           tGGTCGATAAATTATGACCAGGAACAAGGGTACATGCTGTCAAAGACAGAACAGCCAATGTCAGTACCGAAAACTTAAAAATGTTCTTTTTCATTGGTTTATATTTATCAGTCAATGTAATGTTATGAATTACGTCAGGt           >  1:2647717/1‑140 (MQ=255)

                                                                                                                             gTCGATAAATTATGACCAGGAACAAGGGTACATGCTGTCAAAGACAGAACAGCCAATGTCAGTACCGAAAACTTAAAAATGTTCTTTTTCATTGGTTTATATTTATCAGTCAATGTAATGTTATGAATTACGTCAGGTTa         >  1:1973229/1‑140 (MQ=255)

                                                                                                                             gTCGATAAATTATGACCAGGAACAAGGGTACATGCTGTCAAAGACAGAACAGCCAATGTCAGTACCGAAAACTTAAAAATGTTCTTTTTCATTGGTTTATATTTATCAGTCAATGTAATGTTATGAATTACGTCAGGTTa         >  2:2722550/1‑140 (MQ=255)

                                                                                                                             gTCGATAAATTATGACCAGGAACAAGGGTACATGCTGTCAAAGACAGAACAGCCAATGTCAGTACCGAAAACTTAAAAATGTTCTTTTTCATTGGTTTATATTTATCAGTCAATGTAATGTTATGAATTACGTCAGGTTa         >  1:2422418/1‑140 (MQ=255)

                                                                                                                             gTCGATAAATTATGACCAGGAACAAGGGTACATGCTGTCAAAGACAGAACAGCCAATGTCAGTACCGAAAACTTAAAAATGTTCTTTTTCATTGGTTTATATTTATCAGTCAATGTAATGTTATGAATTACGTCAGGTTa         >  2:1845834/1‑140 (MQ=255)

                                                                                                                             gTCGATAAATTATGACCAGGAACAAGGGTACATGCTGTCAAAGACAGAACAGCCAATGTCAGTACCGAAAACTTAAAAATGTTCTTTTTCATTGGTTTATATTTATCAGTCAATGTAATGTTATGAATTACGTCAGGTTa         >  2:178949/1‑140 (MQ=255)

                                                                                                                                 aTAAATTATGACCAGGAACAAGGGTACATGCTGTCAAAGACAGAACAGCCAATGTCAGTACCGAAAACTTAAAAATGTTCTTTTTCATTGGTTTATATTTATCAGTCAATGTAATGTTATGAATTACGTCAGGTTATTGt     <  2:1192939/140‑1 (MQ=255)

                                                                                                                                    aaTTATGACCAGGAACAAGGGTACATGCTGTCAAAGACAGAACAGCCAATGTCAGTACCGAAAACTTAAAAATGTTCTTTTTCATTGGTTTATATTTATCAGTCAATGTAATGTTATGAATTACGTCAGGTTATTGTCCa  <  2:44550/140‑1 (MQ=255)

TAGCCTGCGACATAATCGGTTTGGCGCGCAACTGATCAATCAATCCTGGCGTGACGGGATAGATATTGACCATTTTGTCCAGGTCATACTGATTGTCAGGTAGTTCAATGACATCCTTGTTACTGGTCGATAAATTCTGACCAGGAACAAGGGTACATGCTGTCAAAGACAGAACAGCCAATGTCAGTACCGAAAACTTAAAAATGTTCTTTTTCATTGGTTTATATTTATCAGTCAATGTAATGTTATGAATTACGTCAGGTTATTGTCCA  >  NC_000913/1045587‑1045858

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 26 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`