BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,407,208	C→A	intergenic (‑567/‑353)	lrhA ← / → alaA	DNA‑binding transcriptional dual regulator LrhA/glutamate‑‑pyruvate aminotransferase AlaA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,407,208	0	C	A	99.4%	597.1 / ‑3.2	169	intergenic (‑567/‑353)	lrhA/alaA	DNA‑binding transcriptional dual regulator LrhA/glutamate‑‑pyruvate aminotransferase AlaA
Reads supporting (aligned to +/- strand): ref base C (1/0); new base A (115/53); total (116/53)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.01e-01

Only 100 of 173 total aligned reads displayed.

CAAAGTTATGTTATGTTAGGCAAAGTAATCTTCTATTATTAATAAGCACATCAAAACCTTTTTGAATATTAAATAATAATTAATTAGCATCATCCTCATTCATTAATTCCGCTTAACAATAGTTTCACACCTTGCCCCCATTGCGACACCTCGGAAATCATCACGCAGTTAAACTCTCCGTAATGAGGAAATATCATCATCGCGTTTCATTAGTGAATTCTTAAATGAGGCATTTTCACACAATTATCTTACAGATAAAAAAACCAGACTTAC  >  NC_000913/2407071‑2407343

cAAAGTTATGTTATGTTAGGCAAAGTAATCTTCTATTATTAATAAGCACATCAAAACCTTTTTGAATATTAAATAATAATTAATTAGCATCATCCTCATTCATTAATTCCGCTTAACAATAGTTTCACACCTTGCCcaca                                                                                                                                       <  2:1297458/140‑1 (MQ=255)

       atgttatgttAGGCAAAGTAATCTTCTATTATTAATAAGCACATCAAAACCTTTTTGAATATTAAATAATAATTAATTAGCATCATCCTCATTCATTAATTCCGCTTAACAATAGTTTCACACCTTGCCCACATTGCGac                                                                                                                                <  1:178755/140‑1 (MQ=255)

        tgttatgttaGGCAAAGTAATCTTCTATTATTAATAAGCACATCAAAACCTTTTTGAATATTAAATAATAATTAATTAGCATCATCCTCATTCATTAATTCCGCTTAACAATAGTTTCACACCTTGCCCACATTGCGaca                                                                                                                               >  1:1954999/1‑140 (MQ=255)

             tgttaGGCAAAGTAATCTTCTATTATTAATAAGCACATCAAAACCTTTTTGAATATTAAATAATAATTAATTAGCATCATCCTCATTCATTAATTCCGCTTAACAATAGTTTCACACCTTGCCCACATTGCGACACCTCg                                                                                                                          >  2:30920/1‑140 (MQ=255)

               ttaGGCAAAGTAATCTTCTATTATTAATAAGCACATCAAAACCTTTTTGAATATTAAATAATAATTAATTAGCATCATCCTCATTCATTAATTCCGCTTAACAATAGTTTCACACCTTGCCCACATTGCGACACCTCGGa                                                                                                                        >  1:832690/1‑140 (MQ=255)

               ttaGGCAAAGTAATCTTCTATTATTAATAAGCACATCAAAACCTTTTTGAATATTAAATAATAATTAATTAGCATCATCCTCATTCATTAATTCCGCTTAACAATAGTTTCACACCTTGCCCACATTGCGACACCTCGGa                                                                                                                        >  2:1422705/1‑140 (MQ=255)

                  ggCAAAGTAATCTTCTATTATTAATAAGCACATCAAAACCTTTTTGAATATTAAATAATAATTAATTAGCATCATCCTCATTCATTAATTCCGCTTAACAATAGTTTCACACCTTGCCCACATTGCGACACCTCGGAAat                                                                                                                     >  2:2004644/1‑140 (MQ=255)

                       aGTAATCTTCTATTATTAATAAGCACATCAAAACCTTTTTGAATATTAAATAATAATTAATTAGCATCATCCTCATTCATTAATTCCGCTTAACAATAGTTTCATACCTTGCCCACATTGCGACACCTCGGAAAtcatca                                                                                                                >  2:1143763/1‑140 (MQ=255)

                       aGTAATCTTCTATTATTAATAAGCACATCAAAACCTTTTTGAATATTAAATAATAATTAATTAGCATCATCCTCATTCATTAATTCCGCTTAACAATAGTTTCACACCTTGCCCACATTGCGACACCTCGGAAAtcatca                                                                                                                <  1:1276064/140‑1 (MQ=255)

                       aGTAATCTTCTATTATTAATAAGCACATCAAAACCTTTTTGAATATTAAATAATAATTAATTAGCATCATCCTCATTCATTAATTCCGCTTAACAATAGTTTCACACCTTGCCCACATTGCGACACCTCGGAAAtcatca                                                                                                                >  2:1495461/1‑140 (MQ=255)

                       aGTAATCTTCTATTATTAATAAGCACATCAAAACCTTTTTGAATATTAAATAATAATTAATTAGCATCATCCTCATTCATTAATTCCGCTTAACAATAGTTTCACACCTTGCCCACATTGCGACACCTCGGAAAtcatca                                                                                                                >  1:21271/1‑140 (MQ=255)

                       aGTAATCTTCTATTATTAATAAGCACATCAAAACCTTTTTGAATATTAAATAATAATTAATTAGCATCATCCTCATTCATTAATTCCGCTTAACAATAGTTTCACACCTTGCCCACATTGCGACACCTCGGAAAtcatca                                                                                                                >  2:1698638/1‑140 (MQ=255)

                         tAATCTTCTATTATTAATAAGCACATCAAAACCTTTTTGAATATTAAATAATAATTAATTAGCATCATCCTCATTCATTAATTCCGCTTAACAATAGTTTCACACCTTGCCCACATTGCGACACCTCGGAAATCATCACg                                                                                                              <  2:2127468/140‑1 (MQ=255)

                         tAATCTTCTATTATTAATAAGCACATCAAAACCTTTTTGAATATTAAATAATAATTAATTAGCATCATCCTCATTCATTAATTCCGCTTAACAATAGTTTCACACCTTGCCCACATTGCGACACCTCGGAAATCATCACg                                                                                                              <  1:236496/140‑1 (MQ=255)

                         tAATCTTCTATTATTAATAAGCACATCAAAACCTTTTTGAATATTAAATAATAATTAATTAGCATCATCCTCATTCATTAATTCCGCTTAACAATAGTTTCACACCTTGCCCACATTGCGACACCTCGGAAATCATCACg                                                                                                              >  2:2224293/1‑140 (MQ=255)

                             cttctATTATTAATAAGCACATCAAAACCTTTTTGAATATTAAATAATAATTAATTAGCATCATCCTCATTCATTAATTCCGCTTAACAATAGTTTCACACCTTGCCCACATTGCGACACCTCGGAAATCATCACGCAGt                                                                                                          >  2:2035440/1‑140 (MQ=255)

                                 tattatTAATAAGCACATCAAAACCTTTTTGAATATTAAATAATAATTAATTAGCATCATCCTCATTCATTAATTCCGCTTAACAATAGTTTCACACCTTGCCCACATTGCGACACCTCGGAAATCATCACGCAGTTaaa                                                                                                      >  1:1415051/1‑140 (MQ=255)

                                 tattatTAATAAGCACATCAAAACCTTTTTGAATATTAAATAATAATTAATTAGCATCATCCTCATTCATTAATTCCGCTTAACAATAGTTTCACACCTTGCCCACATTGCGACACCTCGGAAATCATCACGCAGTTaaa                                                                                                      >  2:317304/1‑140 (MQ=255)

                                  attattAATAAGCACATCAAAACCTTTTTGAATATTAAATAATAATTAATTAGCATCATCCTCATTCATTAATTCCGCTTAACAATAGTTTCACACCTTGCCCACATTGCGACACCTCGGAAATCATCACGCAGTTAAAc                                                                                                     <  1:1924517/140‑1 (MQ=255)

                                   ttattaATAAGCACATCAAAACCTTTTTGAATATTAAATAATAATTAATTAGCATCATCCTCATTCATTAATTCCGCTTAACAATAGTTTCACACCTTGCCCACATTGCGACACCTCGGAAATCATCACGCAGTTAAAct                                                                                                    >  2:1848136/1‑140 (MQ=255)

                                   ttattaATAAGCACATCAAAACCTTTTTGAATATTAAATAATAATTAATTAGCATCATCCTCATTCATTAATTCCGCTTAACAATAGTTTCACACCTTGCCCACATTGCGACACCTCGGAAATCATCACGCAGTTAAAct                                                                                                    <  1:1495461/140‑1 (MQ=255)

                                   ttattaATAAGCACATCAAAACCTTTTTGAATATTAAATAATAATTAATTAGCATCATCCTCATTCATTAATTCCGCTTAACAATAGTTTCACACCTTGCCCACATTGCGACACCTCGGAAATCATCACGCAGTTAAAct                                                                                                    >  1:1082172/1‑140 (MQ=255)

                                   ttattaATAAGCACATCAAAACCTTTTTGAATATTAAATAATAATTAATTAGCATCATCCTCATTCATTAATTCCGCTTAACAATAGTTTCACACCTTGCCCACATTGCGACACCTCGGAAATCATCACGCAGTTAAAct                                                                                                    >  1:1070791/1‑140 (MQ=255)

                                   ttattaATAAGCACATCAAAACCTTTTTGAATATTAAATAATAATTAATTAGCATCATCCTCATTCATTAATTACGCTTACCAATAGTTTCACACCTTGCCCACATTGCGACACCTCGGAAATCATCACGCAGTTAAAct                                                                                                    >  1:1920335/1‑140 (MQ=255)

                                      ttaATAAGCACATCAAAACCTTTTTGAATATTAAATAATAATTAATTAGCATCATCCTCATTCATTAATTCCGCTTAACAATAGTTTCACACCTTGCCCACATTGCGACACCTCGGAAATCATCACGCAGTTAAACtctc                                                                                                 >  1:738609/1‑140 (MQ=255)

                                          taaGCACATCAAAACCTTTTTGAATATTAAATAATAATTAATTAGCATCATCCTCATTCATTAATTCCGCTTAACAATAGTTTCACACCTTGCCCACATTGCGACACCTCGGAAATCATCACGCAGTTAAACTCTCCGTa                                                                                             >  2:2104128/1‑140 (MQ=255)

                                            aGCACATCAAAACCTTTTTGAATATTAAATAATAATTAATTAGCATCATCCTCATTCATTAATTCCGCTTAACAATAGTTTCACACCTTGCCCACATTGCGACACCTCGGAAATCATCACGCAGTTAAACTCTCCGTAAt                                                                                           <  2:1082172/140‑1 (MQ=255)

                                               acaTCAAAACCTTTTTGAATATTAAATAATAATTAATTAGCATCATCCTCATTCATTAATTCCGCTTAACAATAGTTTCACACCTTGCCCACATTGCGACACCTCGGAAATCATCACGCAGTTAAACTCTCCGTAATGAg                                                                                        <  1:325794/140‑1 (MQ=255)

                                               acaTCAAAACCTTTTTGAATATTAAATAATAATTAATTAGCATCATCCTCATTCATTAATTCCGCTTAACAATAGTTTCACACCTTGCCCACATTGCGACACCTCGGAAATCATCACGCAGTTAAACTCTCCGTAATGAg                                                                                        <  1:667041/140‑1 (MQ=255)

                                                caTCAAAACCTTTTTGAATATTAAATAATAATTAATTAGCATCATCCTCATTCATTAATTCCGCTTAACAATAGTTTCACACCTTGCCCACATTGCGACACCTCGGAAATCATCACGCAGTTAAACTCTCCGTAATGAgg                                                                                       >  1:179835/1‑140 (MQ=255)

                                                caTCAAAACCTTTTTGAATATTAAATAATAATTAATTAGCATCATCCTCATTCATTAATTCCGCTTAACAATAGTTTCACACCTTGCCCACATTGCGACACCTCGGAAATCATCACGCAGTTAAACTCTCCGTAATGAgg                                                                                       >  1:2023131/1‑140 (MQ=255)

                                                     aaaCCTTTTTGAATATTAAATAATAATTAATTAGCATCATCCTCATTCATTAATTCCGCTTAACAATAGTTTCACACCTTGCCCACATTGCGACACCTCGGAAATCATCACGCAGTTAAACTCTCCGTAATGAGGAAata                                                                                  >  1:1861515/1‑140 (MQ=255)

                                                        ccTTTTTGAATATTAAATAATAATTAATTAGCATCATCCTCATTCATTAATTCCGCTTAACAATAGTTTCACACCTTGCCCACATTGCGACCCCTCGGAAATCATCACGCAGTTAAACTCTCCGTAATGAGGAAATatca                                                                               <  1:363256/140‑1 (MQ=255)

                                                           ttttGAATATTAAATAATAATTAATTAGCATCATCCTCATTCATTAATTCCGCTTAACAATAGTTTCACACCTTGCCCACATTGCGACACCTCGGAAATCATCACGCAGTTAAACTCTCCGTAATGAGGAAATatcatca                                                                            >  1:1839724/1‑140 (MQ=255)

                                                           ttttGAATATTAAATAATAATTAATTAGCATCATCCTCATTCATTAATTCCGCTTAACAATAGTTTCACACCTTGCCCACATTGCGACACCTCGGAAATCATCACGCAGTTAAACTCTCCGTAATGAGGAAATatcatca                                                                            >  2:277081/1‑140 (MQ=255)

                                                           ttttGAATATTAAATAATAATTAATTAGCATCATCCTCATTCATTAATTCCGCTTAACAATAGTTTCACACCTTGCCCACATTGCGACACCTCGGAAATCATCACGCAGTTAAACTCTCCGTAATGAGGAAATatcatca                                                                            >  1:1615450/1‑140 (MQ=255)

                                                           ttttGAATATTAAATAATAATTAATTAGCATCATCCTCATTCATTAATTCCGCTTAACAATAGTTTCACACCTTGCCCACATTGCGACACCTCGGAAATCATCACGCAGTTAAACTCTCCGTAATGAGGAAATatcatca                                                                            >  2:2088495/1‑140 (MQ=255)

                                                           ttttGAATATTAAATAATAATTAATTAGCATCATCCTCATTCATTAATTCCGCTTAACAATAGTTTCACACCTTGCCCACATTGCGACACCTCGGAAATCATCACGCAGTTAAACTCTCCGTAATGAGGAAATatcatca                                                                            >  2:1373519/1‑140 (MQ=255)

                                                            tttGAATATTAAATAATAATTAATTAGCATCATCCTCATTCATTAATTCCGCTTAACAATAGTTTCACACCTTGCCCACATTGCGACACCTCGGAAATCATCACGCAGTTAAACTCTCCGTAATGAGGAAATatcatcat                                                                           <  2:228433/140‑1 (MQ=255)

                                                                aaTATTAAATAATAATTAATTAGCATCATCCTCATTCATTAATTCCGCTTAACAATAGTTTCACACCTTGCCCACATTGCGACACCTCGGAAATCATCACGCAGTTAAACTCTCCGTAATGAGGAAATATCATCATcgcg                                                                       <  1:1934241/140‑1 (MQ=255)

                                                                   atTAAATAATAATTAATTAACATCATCCTCATTCATTAATTCCGCTTAACAATAGTTTCACACCTTGCCCACATTGCGACACCTCGGAAATCATCACGCAGTTAAACTCTCCGTAATGAGGAAAGATCATCATCGCGttt                                                                    >  2:2104614/1‑140 (MQ=255)

                                                                        ataataatTAATTAGCATCATCCTCATTCATTAATTCCGCTTAACAATAGTTTCACACCTTGCCCACATTGCGACACCTCGGAAATCATCACGCAGTTAAACTCTCCGTAATGAGGAAATATCATCATCGCGTTTCATTa                                                               <  2:1415051/140‑1 (MQ=255)

                                                                          aataatTAATTAGCATCATCCTCATTCATTAATTCCGCTTAACAATAGTTTCACACCTTGCCCACATTGCGACACCTCGGAAATCATCACGCAGTTAAACTCTCCGTAATGAGGAAATATCATCATCGCGTTTCATTAGt                                                             <  1:1574735/140‑1 (MQ=255)

                                                                          aataatTAATTAGCATCATCCTCATTCATTAATTCCGCTTAACAATAGTTTCACACCTTGCCCACATTGCGACACCTCGGAAATCATCACGCAGTTAAACTCTCCGTAATGAGGAAATATCATCATCGCGTTTCATTAGt                                                             >  2:1355956/1‑140 (MQ=255)

                                                                            taattaatTAGCATCATCCTCATTCATTAATTCCGCTTAACAATAGTTTCACACCTTGCCCACATTGCGACACCTCGGAAATCATCACGCAGTTAAACTCTCCGTAATGAGGAAATATCATCATCGCGTTTCATTAGTGa                                                           >  1:786987/1‑140 (MQ=255)

                                                                               ttaattaGCATCATCCTCATTCATTAATTCCGCTTAACAATAGTTTCACACCTTGCCCACATTGTGACACCTCGGAAATCATCACGCAGTTAAACTCTCCGTAATGAGGAAATATCATCATCGCGTTTCATTAGTGAAtt                                                        >  1:953307/1‑140 (MQ=255)

                                                                               ttaattaGCATCATCCTCATTCATTAATTCCGCTTAACAATAGTTTCACACCTTGCCCACATTGCGACACCTCGGAAATCATCACGCAGTTAAACTCTCCGTAATGAGGAAATATCATCATCGCGTTTCATTAGTGAAtt                                                        >  1:2208369/1‑140 (MQ=255)

                                                                               ttaattaGCATCATCCTCATTCATTAATTCCGCTTAACAATAGTTTCACACCTTGCCCACATTGCGACACCTCGGAAATCATCACGCAGTTAAACTCTCCGTAATGAGGAAATATCATCATCGCGTTTCATTAGTGAAtt                                                        <  2:1812103/140‑1 (MQ=255)

                                                                                    taGCATCATCCTCATTCATTAATTCCGCTTAACAATAGTTTCACACCTTGCCCACATTGCGACACCTCGGAAATCATCACGCAGTTAAACTCTCCTTAATGAGGAAATATCATCATCGCGTTTCATTAGTGAATTCTTaa                                                   >  2:702465/1‑140 (MQ=255)

                                                                                      gCATCATCCTCATTCATTAATTCCGCTTAACAATAGTTTCACACCTTGCCCACATTGCGACACCTCGGAAATCATCACGCAGTTAAACTCTCCGTAATGAGGAAATATCATCATCGCGTTTCATTAGTGAATTCTTAAAt                                                 >  1:1772800/1‑140 (MQ=255)

                                                                                      gCATCATCCTCATTCATTAATTCCGCTTAACAATAGTTTCACACCTTGCCCACATTGCGACACCTCGGAAATCATCACGCAGTTAAACTCTCCGTAATGAGGAAATATCATCATCGCGTTTCATTAGTGAATTCTTAAAt                                                 >  2:491069/1‑140 (MQ=255)

                                                                                       catcatCCTCATTCATTAATTCCGCTTAACAATAGTTTCACACCTTGCCCACATTGCGACACCTCGGAAATCATCACGCAGTTAAACTCTCCGTAATGAGGAAATATCATCATCGCGTTTCATTAGTGAATTCTTAAATg                                                >  2:1525692/1‑140 (MQ=255)

                                                                                       catcatCCTCATTCATTAATTCCGCTTAACAATAGTTTCACACCTTGCCCACATTGCGACACCTCGGAAATCATCACGCAGTTAAACTCTCCGTAATGAGGAAATATCATCATCGCGTTTCATTAGTGAATTCTTAAATg                                                >  1:2100083/1‑140 (MQ=255)

                                                                                       catcatCCTCATTCATTAATTCCGCTTAACAATAGTTTCACACCTTGCCCACATTGCGACACCTCGGAAATCATCACGCAGTTAAACTCTCCGTAATGAGGAAATATCATCATCGCGTTTCATTAGTGAATTCTTAAATg                                                >  2:526641/1‑140 (MQ=255)

                                                                                       catcatCCTCATTCATTAATTCCGCTTAACAATAGTTTCACACCTTGCCCACATTGCGACACCTCGGAAATCATCACGCAGTTAAACTCTCCGTAATGAGGAAATATCATCATCGCGTTTCATTAGTGAATTCTTAAATg                                                >  2:1756126/1‑140 (MQ=255)

                                                                                          catcCTCATTCATTAATTCCGCTTAACAATAGTTTCACACCTTGCCCACATTGCGACACCTCGGAAATCATCACGCAGTTAAACTCTCCGTAATGAGGAAATATGATCATCGCGTTTCATTAGTGAATTCTTAAATGAgg                                             <  2:1610308/140‑1 (MQ=255)

                                                                                          catcCTCATTCATTAATTCCGCTTAACAATAGTTTCACACCTTGCCCACATTGCGACACCTCGGAAATCATCACGCAGTTAAACTCTCCGTAATGAGGAAATATCATCATCGCGTTTCATTAGTGAATTCTTAAATGAgg                                             <  2:1858646/140‑1 (MQ=255)

                                                                                          catcCTCATTCATTAATTCCGCTTAACAATAGTTTCACACCTTGCCCACATTGCGACACCTCGGAAATCATCACGCAGTTAAACTCTCCGTAATGAGGAAATATCATCATCGCGTTTCATTAGTGAATTCTTAAATGAgg                                             <  2:1565549/140‑1 (MQ=255)

                                                                                            tcCTCATTCATTAATTCCGCTTAACAATAGTTTCACACCTTGCCCACATTGCGACACCTCGGAAATCATCACGCAGTTAAACTCTCCGTAATGAGGAAATATCATCATCGCGTTTCATTAGTGAATTCTTAAATGAGGCa                                           >  1:696634/1‑140 (MQ=255)

                                                                                            tcCTCATTCATTAATTCCGCTTAACAATAGTTTCACACCTTGCCCACATTGCGACACCTCGGAAATCATCACGCAGTTAAACTCTCCGTAATGAGGAAATATCATCATCGCGTTTCATTAGTGAATTCTTAAATGAGGCa                                           >  1:1230201/1‑140 (MQ=255)

                                                                                                cattcattAATTCCGCTTAACAATAGTTTCACACCTTGCCCACATTGCGACACCTCGGAAATCATCACGCAGTTAAACTCTCCGTAATGAGGAAATATCATCATCGCGTTTCATTAGTGAATTCTTAAATGAGGCAtttt                                       >  2:1084573/1‑140 (MQ=255)

                                                                                                 attcattAATTCCGCTTAACAATAGTTTCACACCTTGCCCACATTGCGACACCTCGGAAATCATCACGCAGTTAAACTCTCCGTAATGAGGAAATATCATCATCGCGTTTCATTAGTGAATTCTTAAATGAGGCATTTTc                                      <  1:1698638/140‑1 (MQ=255)

                                                                                                 attcattAATTCCGCTTAACAATAGTTTCACACCTTGCCCACATTGCGACACCTCGGAAATCATCACGCAGTTAAACTCTCCGTAATGAGGAAATATCATCATCGCGTTTCATTAGTGAATTCTTAAATGAGGCATTTTc                                      >  1:1295414/1‑140 (MQ=255)

                                                                                                 attcattAATTCCGCTTAACAATAGTTTCACACCTTGCCCACATTGCGACACCTCGGAAATCATCACGCAGTTAAACTCTCCGTAATGAGGAAATATCATCATCGCGTTTCATTAGTGAATTCTTAAATGAGGCATTTTc                                      >  1:1880953/1‑140 (MQ=255)

                                                                                                 attcattAATTCCGCTTAACAATAGTTTCACACCTTGCCCACATTGCGACACCTCGGAAATCATCACGCAGTTAAACTCTCCGTAATGAGGAAATATCATCATCGCGTTTCATTAGTGAATTCTTAAATGAGGCATTTTc                                      <  1:620259/140‑1 (MQ=255)

                                                                                                   tcattAATTCCGCTTAACAATAGTTTCACACCTTGCCCACATTGCGACACCTCGGAAATCATCACGCAGTTAAACTCTCCGTAATGAGGAAATATCATCATCGCGTTTCATTAGTGAATTCTTAAATGAGGCATTTTcac                                    <  1:465329/140‑1 (MQ=255)

                                                                                                     attAATTCCGCTTAACAATAGTTTCATACCTTGCCCACATTGCGACACCTCGGAAATCATCACGCAGTTAAACTCTCCGTAATGAGGAAATATCATCATCGCGTTTCATTAGTGAATTCTTAAATGAGGCATTTTcacac                                  <  1:1143763/140‑1 (MQ=255)

                                                                                                     attAATTCCGCTTAACAATAGTTTCACACCTTGCCCACATTGCGACACCTCGGAAATCATCACGCAGTTAAACTCTCCGTAATGAGGAAATATCATCATCGCGTTTCATTAGTGAATTCTTAAATGAGGCATTTTcacac                                  <  1:1035619/140‑1 (MQ=255)

                                                                                                     attAATTCCGCTTAACAATAGTTTCACACCTTGCCCACATTGCGACACCTCGGAAATCATCACGCAGTTAAACTCTCCGTAATGAGGAAATATCATCATCGCGTTTCATTAGTGAATTCTTAAATGAGGCATTTTcacac                                  <  2:826639/140‑1 (MQ=255)

                                                                                                     attAATTCCGCTTAACAATAGTTTCACACCTTGCCCACATTGCGACACCTCGGAAATCATCACGCAGTTAAACTCTCCGTAATGAGGAAATATCATCATCGCGTTTCATTAGTGAATTCTTAAATGAGGCATTTTcacac                                  <  1:2004644/140‑1 (MQ=255)

                                                                                                     attAATTCCGCTTAACAATAGTTTCACACCTTGCCCACATTGCGACACCTCGGAAATCATCACGCAGTTAAACTCTCCGTAATGAGGAAATATCATCATCGCGTTTCATTAGTGAATTCTTAAATGAGGCATTTTcacac                                  >  1:2192560/1‑140 (MQ=255)

                                                                                                     attAATTCCGCTTAACAATAGTTTCACACCTTGCCCACATTGCGACACCTCGGAAATCATCACGCAGTTAAACTCTCCGTAATGAGGAAATATCATCATCGCGTTTCATTAGTGAATTCTTAAATGAGGCATTTTcacac                                  <  1:277081/140‑1 (MQ=255)

                                                                                                      ttAATTCCGCTTAACAATAGTTTCACACCTTGCCCACATTGCGACACCTCGGAAATCATCACGCAGTTAAACTCTCCGTAATGAGGAAATATCATCATCGCGTTTCATTAGTGAATTCTTAAATGAGGCATTTTcacaca                                 >  1:41323/1‑140 (MQ=255)

                                                                                                       tAATTCCGCTTAACAATAGTTTCACACCTTGCCCACATTGCGACACCTCGGAAATCATCACGCAGTTAAACTCTCCGTAATGAGGAAATATCATCATCGCGTTTCATTAGTGAATTCTTAAATTAGGCATTTTCACACaa                                <  2:1968239/140‑1 (MQ=255)

                                                                                                       tAATTCCGCTTAACAATAGTTTCACACCTTGCCCACATTGCGACACCTCGGAAATCATCACGCAGTTAAACTCTCCGTAATGAGGAAATATCATCATCGCGTTTCATTAGTGAATTCTTAAATGAGGCATTTTCACACaa                                >  2:164657/1‑140 (MQ=255)

                                                                                                       tAATTCCGCTTAACAATAGTTTCACACCTTGCCCACATTGCGACACCTCGGAAATCATCACGCAGTTAAACTCTCCGTAATGAGGAAATATCATCATCGCGTTTCATTAGTGAATTCTTAAATGAGGCATTTTCACACaa                                <  2:1351217/140‑1 (MQ=255)

                                                                                                       tAATTCCGCTTAACAATAGTTTCACACCTTGCCCACATTGCGACACCTCGGAAATCATCACGCAGCTAAACTCTCCGTAATGAGGAAATATCATCATCGCGTTTCATTAGTGAATTCTTAAATGAGGCATTTTCACACaa                                <  2:597562/140‑1 (MQ=255)

                                                                                                             cGCTTAACAATAGTTTCACACCTTGCCCACATTGCGACACCTCGGAAATCATCACGCAGTTAAACTCTCCGTAATGAGGAAATATCATCATCGCGTTTCATTAGTGAATTCTTAAATGAGGCATTTTCACACAATTATCt                          >  1:1648445/1‑140 (MQ=255)

                                                                                                             cGCTTAACAATAGTTTCACACCTTGCCCACATTGCGACACCTCGGAAATCATCACGCAGTTAAACTCTCCGTAATGAGGAAATATCATCATCGCGTTTCATTAGTGAATTCTTAAATGAGGCATTTTCACACAATTATCt                          >  2:1669807/1‑140 (MQ=255)

                                                                                                             cGCTTAACAATAGTTTCACACCTTGCCCACATTGCGACACCTCGGAAATCATCACGCAGTTAAACTCTCCGTAATGAGGAAATATCATCATCGCGTTTCATTAGTGAATTCTTAAATGAGGCATTTTCACACAATTATCt                          >  2:23200/1‑140 (MQ=255)

                                                                                                             cGCTTAACAATAGTTTCACACCTTGCCCACATTGCGACACCTCGGAAATCATCACGCAGTTAAACTCTCCGTAATGAGGAAATATCATCATCGCGTTTAATTAGTGAATTCTTAAATGAGGCATTTTCACACAATTATCt                          >  1:719748/1‑140 (MQ=255)

                                                                                                               cTTAACAATAGTTTCACACCTTGCCCACATTGCGACACCTCGGAAATCATCACGCAGTTAAACTCTCCGTAATGAGGAAATATCATCATCGCGTTTCATTAGTGAATTCTTAAATGAGGCATTTTCACACAATTATCTTa                        >  1:41148/1‑140 (MQ=255)

                                                                                                               cTTAACAATAGTTTCACACCTTGCCCACATTGCGACACCTCGGAAATCATCACGCAGTTAAACTCTCCGTAATGAGGAAATATCATCATCGCGTTTCATTAGTGAATTCTTAAATGAGGCATTTTCACACAATTATCTTa                        >  1:999046/1‑140 (MQ=255)

                                                                                                               cTTAACAATAGTTTCACACCTTGCCCACATTGCGACACCTCGGAAATCATCACGCAGTTAAACTCTCCGTAATGAGGAAATATCATCATCGCGTTTCATTAGTGAATTCTTAAATGAGGCATTTTCACACAATTATCTTa                        >  2:1841157/1‑140 (MQ=255)

                                                                                                               cTTAACAATAGTTTCACACCTTGCCCACATTGCGACACCTCGGAAATCATCACGCAGTTAAACTCTCCGTAATGAGGAAATATCATCATCGCGTTTCATTAGTGAATTCTTAAATGAGGCATTTTCACACAATTATCTTa                        >  2:835049/1‑140 (MQ=255)

                                                                                                               cTTAACAATAGTTTCACACCTTGCCCACATTGCGACACCTCGGAAATCATCACGCAGTTAAACTCTCCGTAATGAGGAAATATCATCATCGCGTTTCATTAGTGAATTCTTAAATGAGGCATTTTCACACAATTATCTTa                        <  2:786987/140‑1 (MQ=255)

                                                                                                                 tAACAATAGTTTCACACCTTGCCCACATTGCGACACCTCGGAAATCATCACGCAGTTAAACTCTCCGTAATGAGGAAATATCATCATCGCGTTTCATTAGTGAATTCTTAAATGAGGCATTTTCACACAATTATCTTACa                      >  1:1489095/1‑140 (MQ=255)

                                                                                                                    cAATAGTTTCACACCTTGCCCACATTGCGACACCTCGGAAATCATCACGCAGTTAAACTCTCCGTAATGAGGAAATATCATCATCGCGTTTCATTAGTGAATTCTTAAATGAGGCATTTTCACACAATTATCTTACAGAt                   >  2:368960/1‑140 (MQ=255)

                                                                                                                          tttCACACCTTGCCCACATTGCGACACCTCGGAAATCATCACGCAGTTAAACTCTCCGTAATGAGGAAATATCATCATCGCGTTTCATTAGTGAATTCTTAAATGAGGCATTTTCACACAATTATCTTACAGATaaaaaa             >  1:63083/1‑140 (MQ=255)

                                                                                                                            tCACACCTTGCCCACATTGCGACACCTCGGAAATCATCACGCAGTTAAACTCTCCGTAATGAGGAAATATCATCATCGCGTTTCATTAGTGAATTCTTAAATGAGGCATTTTCACACAATTATCTTACAGATAAAAAAAc           <  1:526641/140‑1 (MQ=255)

                                                                                                                            tCACACCTTGCCCACATTGCGACACCTCGGAAATCATCACGCAGTTAAACTCTCCGTAATGAGGAAATATCATCATCGCGTTTCATTAGTGAATTCTTAAATGAGGCATTTTCACACAATTATCTTACAGATAAAAAAAc           <  1:835049/140‑1 (MQ=255)

                                                                                                                             cacaCCTTGCCCACATTGCGACACCTCGGAAATCATCACGCAGTTAAACTCTCCGTAATGAGGAAATATCATCATCGCGTTTCATTAGTGAATTCTTAAATGAGGCATTTTCACACAATTATCTTACAGATAAAAAAAcc          <  1:1366327/140‑1 (MQ=255)

                                                                                                                              acacCTTGCCCACATTGCGACACCTCGGAAATCATCACGCAGTTAAACTCTCCGTAATGAGGAAATATCATCATCGCGTTTCATTAGTGAATTCTTAAATGAGGCATTTTCACACAATTATCTTACAGATAAAAAAACCa         >  1:1022815/1‑140 (MQ=255)

                                                                                                                               cacCTTGCCCACATTGCGACACCTCGGAAATCATCACGCAGTTAAACTCTCCGTAATGAGGAAATATCATCATCGCGTTTCATTAGTGAATTCTTAAATGAGGCATTTTCACACAATTATCTTACAGATAAAAAAACCAg        >  1:2029928/1‑140 (MQ=255)

                                                                                                                                 ccTTGCCCACATTGCGACACCTCGGAAATCATCACGCAGTTAAACTCTCCGTAATGAGGAAATATCATCATCGCGTCTCATTAGTGAATTCTTAAATGAGGCATTTTCACACAATTATCTTACAGATAAAAAAACCAGAc      <  1:1096662/140‑1 (MQ=255)

                                                                                                                                  cTTGCCCCCATTGCGACACCTCGGAAATCATCACGCAGTTAAACTCTCCGTAATGAGGAAATATCATCATCGCGTTTCATTAGTGAATTCTTAAATGAGGCATTTTCACACAATTATCTTACAGATAAAAAAACCAGACt     >  1:329188/1‑140 (MQ=255)

                                                                                                                                    tGCCCACATTGCGACACCTCGGAAATCATCACGCAGTTAAACTCTCCGTAATGAGGAAATATCATCATCGCGTTTCATTAGTGAATTCTTAAATGAGGCATTTTCACACAATTATCTTACAGATAAAAAAACCAGACTTa   >  2:931598/1‑140 (MQ=255)

                                                                                                                                     gCCCACATTGCGACACCTCGGAAATCATCACGCAGTTAAACTCTCCGTAATGAGGAAATATCATCATCGCGTTTCATTAGTGAATTCTTAAATGAGGCGTTTTCACACAATTATCTTACAGATAAAAAAAc           <  1:1880645/131‑1 (MQ=255)

                                                                                                                                     gCCCACATTGCGACACCTCGGAAATCATCACGCAGTTAAACTCTCCGTAATGAGGAAATATCATCATCGCGTTTCATTAGTGAATTCTTAAATGAGGCGTTTTCACACAATTATCTTACAGATAAAAAAAc           >  2:1880645/1‑131 (MQ=255)

                                                                                                                                     gCCCACATTGCGACACCTCGGAAATCATCACGCAGTTAAACTCTCCGTAATGAGGAAATATCATCATCGCGTTTCATTAGTGAATTCTTAAATGAGGCATTTTCACACAATTATCTTACAGATAAAAAAACCAGACTTAc  >  2:437973/1‑140 (MQ=255)

CAAAGTTATGTTATGTTAGGCAAAGTAATCTTCTATTATTAATAAGCACATCAAAACCTTTTTGAATATTAAATAATAATTAATTAGCATCATCCTCATTCATTAATTCCGCTTAACAATAGTTTCACACCTTGCCCCCATTGCGACACCTCGGAAATCATCACGCAGTTAAACTCTCCGTAATGAGGAAATATCATCATCGCGTTTCATTAGTGAATTCTTAAATGAGGCATTTTCACACAATTATCTTACAGATAAAAAAACCAGACTTAC  >  NC_000913/2407071‑2407343

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`