Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,688,254	C→A	G307V (GGG→GTG)	bcsC ←	cellulose biosynthesis protein BcsC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,688,254	0	C	A	98.4%	209.3 / NA	61	G307V (GGG→GTG)	bcsC	cellulose biosynthesis protein BcsC
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base A (46/14);  minor base G (1/0);  total (47/14)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CAGTAGCGGTTTACTTTCAGCAGACTGTTCCATTTGTCGTTGTTGCTGCTGTGCGGGTCCAGTGCGAGGGCTTTTTCCAGATTCGCCACTGCATTGGCGCGATCGCCTTTCTGAGAATACGCCTGGCCCAGCGCCCCCAGAGCTTCACTGTCTTTCGGGTTCGCCCGCACCGCCTGTTGTAGTTCGGG‑AATGGCTTTACCCGCCATACCAGAGTCCACCGCCGCTAAACCTTGCGCACGAGCGCGGAAAGCAGGATCGGCCAGCTGTTTT  >  NC_000913/3688119‑3688388                                                                                                                                        |                                                                                                                                        cagTAGCGGTTTACTTTCAGCAGACTGTTCCATTTGTCGTTGTTGCTGCTGTGCGGGTCCAGTGCGAGGGCTTTTTCCAGATTCGCCACTGCATTGGCGCGATCGCCTTTCTGAGAATACGCCTGGCCCAGCGCCACCag                                                                                                                                     >  1:1836168/1‑140 (MQ=255) cagTAGCGGTTTACTTTCAGCAGACTGTTCCA‑TTGTCGTTGTTGCTGCTGTGCGGGTCCAGTGCGAGGGCTTTTTCCAGATTCGCCACTGCATTGGCGCGATCGCCTTTCTGAGAATACGCCTGGCCCAGCGCCACCaga                                                                                                                                    >  2:796617/1‑140 (MQ=255)     aGCGGTTTACTTTCAGCAGACTGTTCCATTTGTCGTTGTTGCTGCTGTGCGGGTCCAGTGCGAGGGCTTTTTCCAGATTCGCCACTGCATTGGCGCGATCGCCTTTCTGAGAATACGCCTGGCCCAGCGCCACCAGAGCt                                                                                                                                 <  2:1416580/140‑1 (MQ=255)        ggTTTACTTTCAGCAGACTGTTCCATTTGTCGTTGTTGCTGCTGTGCGGGTCCAGTGCGAGGGCTTTTTCCAGATTCGCCACTGCATTGGCGCGATCGCCTTTCTGAGAATACGCCTGGCCCAGCGCCACCAGAGCTTCa                                                                                                                              <  2:1718256/140‑1 (MQ=255)          tttACTTTCAGCAGACTGTTCCATTTGTCGTTGTTGCTGCTGTGCGGGTCCAGTGCGAGGGCTTTTTCCAGATTCGCCACTGCATTGGCGCGATCGCCTTTCTGAGAATACGCCTGGCCCAGCGCCACCAGAGCTTCACt                                                                                                                            <  1:392642/140‑1 (MQ=255)           ttACTTTCAGCAGACTGTTCCATTTGTCGTTGTTGCTGCTGTGCGGGTCCAGTGCGAGGGCTTTTTCCAGATTCGCCTCTGCATTGGCGCGATCGCCTTTCTGAGAATACGCCTGGCCCAGCGCCACCAGAGCTTCACTg                                                                                                                           <  1:1964110/140‑1 (MQ=255)                 tCAGCAGACTGTTCCATTTGTCGTTGTTGCTGCTGTGCGGGTCCAGTGCGAGGGCTTTTTCCAGATTCGCCACTGCATTGGCGCGATCGCCTTTCTGAGAATACGCCTGGCCCAGCGCCACCAGAGCTTCACTGTCTTTc                                                                                                                     >  1:18178/1‑140 (MQ=255)                   agcagACTGTTCCATTTGTCGTTGTTGCTGCTGTGCGGGTCCAGTGCGAGGGCTTTTTCCAGATTCGCCACTGCATTGGCGCGATCGCCTTTCTGAGAATACGCCTGGCCCAGCGCCACCAGAGCTTCACTGTCTTTCgg                                                                                                                   >  1:1805621/1‑140 (MQ=255)                    gcagACTGTTCCATTTGTCGTTGTTGCTGCTGTGCGGGTCCAGTGCGAGGGCTTTTTCCAGATTCGCCACTGCATTGGCGCGATCGCCTTTCTGAGAATACGCCTGGCCCAGCGCCACCAGAGCTTCACTGTCTTTCggg                                                                                                                  >  1:1111574/1‑140 (MQ=255)                    gcagACTGTTCCATTTGTCGTTGTTGCTGCTGTGCGGGTCCAGTGCGAGGGCTTTTTCCAGATTCGCCACTGCATTGGCGCGATCGCCTTTCTGAGAATACGCCTGGCCCAGCGCCACCAGAGCTTCACTGTCTTTCggg                                                                                                                  <  2:2127571/140‑1 (MQ=255)                    gcagACTGTTCCATTTGTCGTTGTTGCTGCTGTGCGGGTCCAGTGCGAGGGCTTTTTCCAGATTCGCCACTGCATTGGCGCGATCGCCTTTCTGAGAATACGCCTGGCCCAGCGCCACCAGAGCTTCACTGTCTTTCggg                                                                                                                  <  1:2122857/140‑1 (MQ=255)                        aCTGTTCCATTTGTCGTTGTTGCTGCTGTGCGGGTCCAGTGCGAGGGCTTTTTCCAGATTCGCCACTGCATTGGCGCGATCGCCTTTCTGAGAATACGCCTGGCCCAGCGCCACCAGAGCTTCACTGTCTTTCGGGTTCg                                                                                                              >  2:835475/1‑140 (MQ=255)                        aCTGTTCCATTTGTCGTTGTTGCTGCTGTGCGGGTCCAGTGCGAGGGCTTTTTCCAGATTCGCCACTGCATTGGCGCGATCGCCTTTCTGAGAATACGCCTGGCCCAGCGCCACCAGAGCTTCACTGTCTTTCGGGTTCg                                                                                                              >  1:2002639/1‑140 (MQ=255)                         ttGTTCCATTTGTCGTTGTTGCTGCTGTGCGGGTCCAGTGCGAGGGCTTTTCCCAGATTCGCCCCTGCTTTGGCGCGATCGCCTTTGGGAGAATACGCCTGGCCGAGCCCCCCCAGAGCTTCATTGGCTTTCGGTTTCGc                                                                                                             <  2:1976137/139‑1 (MQ=255)                           gTTCCATTTGTCGTTGTTGCTGCTGTGCGGGTCCAGTGCGAGGGCTTTTTCCAGATTCGCCACTGCATTGGCGCGATCGCCTTTCTGAGAATACGCCTGGCCCAGCGCCACCAGAGCTTCACTGTCTTTCGGGTTCGccc                                                                                                           >  1:1311960/1‑140 (MQ=255)                            ttCCATTTGTCGTTGTTGCTGCTGTGCGGGTCCAGTGCGAGGGCTTTTTCCAGATTCGCCACTGCATTGGCGCGATCGCCTTTCTGAGAATACGCCTGGCCCAGCGCCACCAGAGCTTCACTGTCTTTCGGGTTCGCCCg                                                                                                          >  1:1700834/1‑140 (MQ=255)                             tCCATTTGTCGTTGTTGCTGCTGTGCGGGTCCAGTGCGAGGGCTTTTTCCAGATTCGCCACTGCATTGGCGCGATCGCCTTTCTGAGAATACGCCTGGCCCAGCGCCACCAGAGCTTCACTGTCTTTCGGGTTCGCCCGc                                                                                                         <  1:835475/140‑1 (MQ=255)                             tCCATTTGTCGTTGTTGCTGCTGTGCGGGTCCAGTGCGAGGGCTTTTTCCAGATTCGCCACTGCATTGGCGCGATCGCCTTTCTGAGAATACGCCTGGCCCAGCGCCACCAGAGCTTCACTGTCTTTCGGGTTCGCCCGc                                                                                                         >  2:2096204/1‑140 (MQ=255)                              ccATTTGTCGTTGTTGCTGCTGTGCGGGTCCAGTGCGAGGGCTTTTTCCAGATTCGCCACTGCATTGGCGCGATCGCCTTTCTGAGAATACGCCTGGCCCAGCGCCACCAGAGCTTCACTGTCTTTCGGGTTCGCCCGCa                                                                                                        <  1:2187712/140‑1 (MQ=255)                              ccATTTGTCGTTGTTGCTGCTGTGCGGGTCCAGTGCGAGGGCTTTTTCCAGATTCGCCACTGCATTGGCGCGATCGCCTTTCTGAGAATACGCCTGGCCCAGCGCCACCAGAGCTTCACTGTCTTTCGGGTTCGCCCGCa                                                                                                        <  2:1805621/140‑1 (MQ=255)                                aTTTGTCGTTGTTGCTGCTGTGCGGGTCCAGTGCGAGGGCTTTTTCCAGATTCGCCACTGCATTGGCGCGATCGCCTTTCTGAGAATACGCCTGGCCCAGCGCCACCAGAGCTTCACTGTCTTTCGGGTTCGCCCGCAcc                                                                                                      <  2:1710256/140‑1 (MQ=255)                                aTTTGTCGTTGTTGCTGCTGTGCGGGTCCAGTGCGAGGGCTTTTTCCAGATTCGCCACTGCATTGGCGCGATCGCCTTTCTGAGAATACGCCTGGCCCAGCGCCACCAGAGCTTCACTGTCTTTCGGGTTCGCCCGCAcc                                                                                                      <  1:924372/140‑1 (MQ=255)                                  ttGTCGTTGTTGCTGCTGTGCGGGTCCAGTGCGAGGGCTTTTTCCAGATTCGCCACTGCATTGGCGCGATCGCCTTTCTGAGAATACGCCTGGCCCAGCGCCACCAGAGCTTCACTGTCTTTCGGGTTCGCCCGCACCGc                                                                                                    <  1:193352/140‑1 (MQ=255)                                    gTCGTTGTTGCTGCTGTGCGGGTCCAGTGCGAGGGCTTTTTCCAGATTCGCCACTGCATTGGCGCGATCGCCTTTCTGAGAATACGCCTGGCCCAGCGCCACCAGAGCTTCACTGTCTTTCGGGTTCGCCCGCACCGc                                                                                                    <  2:405882/138‑1 (MQ=255)                                    gTCGTTGTTGCTGCTGTGCGGGTCCAGTGCGAGGGCTTTTTCCAGATTCGCCACTGCATTGGCGCGATCGCCTTTCTGAGAATACGCCTGGCCCAGCGCCACCAGAGCTTCACTGTCTTTCGGGTTCGCCCGCACCGc                                                                                                    >  1:405882/1‑138 (MQ=255)                                    gTCGTTGTTGCTGCTGTGCGGGTCCAGTGCGAGGGCTTTTTCCAGATTCGCCACTGCATTGGCGCGATCGCCTTTCTGAGAATACGCCTGGCCCAGCGCCACCAGAGCTTCACTGTCTTTCGGGTTCGCCCGCACCGCCt                                                                                                  >  1:781766/1‑140 (MQ=255)                                    gTCGTTGTTGCTGCTGTGCGGGTCCAGTGCGAGGGCTTTTTCCAGATTCGCCACTGCATTGGCGCGATCGCCTTTCTGAGAATACGCCTGGCCCAGCGCCACCAGAGCTTCACTGTCTTTCGGGTTCGCCCGCACCGCCt                                                                                                  >  1:695854/1‑140 (MQ=255)                                      cGTTGTTGCTGCTGTGCGGGTCCAGTGCGAGGGCTTTTTCCAGATTCGCCACTGCATTGGCGCGATCGCCTTTCTGAGAATACGCCTGGCCCAGCGCCACCAGAGCTTCACTGTCTTTCGGGTTCGCCCGCACCGCCtgt                                                                                                >  1:1305961/1‑140 (MQ=255)                                      cGTTGTTGCTGCTGTGCGGGTCCAGTGCGAGGGCTTTTTCCAGATTCGCCACTGCATTGGCGCGATCGCCTTTCTGAGAATACGCCTGGCCCAGCGCCACCAGAGCTTCACTGTCTTTCGGGTTCGCCCGCACCGCCtgt                                                                                                >  2:1656104/1‑140 (MQ=255)                                        ttgttgCTGCTGTGCGGGTCCAGTGCGAGGGCTTTTTCCAGATTCGCCACTGCATTGGCGCGATCGCCTTTCTGAGAATACGCCTGGCCCAGCGCCACCAGAGCTTCACTGTCTTTCGGGTTCGCCCGCACCGCCtgttg                                                                                              <  2:511469/140‑1 (MQ=255)                                               tgctgTGCGGGTCCAGTGCGAGGGCTTTTTCCAGATTCGCCACTGCATTGGCGCGATCGCCTTTCTGAGAATACGCCTGGTCCAGCGCCACCAGAGCTTCACTGTCTTTCGGGTTCGCCCGCACCGCCTGTTGTAGTTCg                                                                                       >  1:1448321/1‑140 (MQ=255)                                                    tgCGGGTCCAGTGCGAGGGCTTTTTCCAGATTCGCCACTGCATTGGCGCGATCGCCTTTCTGAGAATACGCCTGGCCCAGCGCCACCAGAGCTTCACTGTCTTTCGGGTTCGCCCGCACCGCCTGTTGTAGTTCGGG‑AAt                                                                                 >  1:363249/1‑140 (MQ=255)                                                      cGGGTCCAGTGCGAGGTCTTTTTCCAGATTCGCCACTGCATTGGCGCGATCGCCTTTCTGAGAATACGCCTGGCCCAGCGCCACCAGAGCTTCACTGTCTTTCGGGTTCGCCCGCACCGCCTGTTGTAGTTCGGG‑AATgg                                                                               >  2:78891/1‑140 (MQ=255)                                                         gTCCAGTGCGAGGGCTTTTTCCAGATTCGCCACTGCATTGGCGCGATCGCCTTTCTGAGAATACGCCTGGCCCAGCGCCACCAGAGCTTCACTGTCTTTCGGGTTCGCCCGCACCGCCTGTTGTAGTTCGGG‑AATGGCtt                                                                            >  1:597943/1‑140 (MQ=255)                                                           ccAGTGCGAGGGCTTTTTCCAGATTCGCCACTGCATTGGCGCGATCGCCTTTCTGAGAATACGCCTGGCCCAGCGCCACCAGAGCTTCACTGTCTTTCGGGTTCGCCCGCACCGCCTGTTGTAGTTCGGG‑AATGGCTTTa                                                                          >  2:1867143/1‑140 (MQ=255)                                                              gTGCGAGGGCTTTTTCCAGATTCGCCACTGCATTGGCGCGATCGCCTTTCTGAGAATACGCCTGGCCCAGCGCCACCAGAGCTTCACTGTCTTTCGGGTTCGCCCGCACCGCCTGTTGTAGTTCGGG‑AATGGCTTTAccc                                                                       >  2:2008881/1‑140 (MQ=255)                                                              gTGCGAGGGCTTTTTCCAGATTCGCCACTGCATTGGCGCGATCGCCTTTCTGAGAATACGCCTGGCCCAGCGCCACCAGAGCTTCACTGTCTTTCGGGTTCGCCCGCACCGCCTGTTGTAGTTCGGG‑AATGGCTTTAccc                                                                       >  1:62541/1‑140 (MQ=255)                                                              gTGCGAGGGCTTTTTCCAGATTCGCCACTGCATTGGCGCGATCGCCTTTCTGAGAATACGCCTGGCCCAGCGCCACCAGAGCTTCACTGTCTTTCGGGTTCGCCCGCACCGCCTGTTGTAGTTCGGG‑AATGGCTTTAccc                                                                       >  1:212291/1‑140 (MQ=255)                                                              gTGCGAGGGCTTTTTCCAGATTCGCCACTGCATTGGCGCGATCGCCTTTCTGAGAATACGCCTGGCCCAGCGCCACCAGAGCTTCACTGTCTTTCGGGTTCGCCCGCACCGCCTGTTGTAGTTCGGG‑AATGGCTTTAccc                                                                       >  1:82024/1‑140 (MQ=255)                                                                   aGGGCTTTTTCCAGATTCGCCACTGCATTGGCGCGATCGCCTTTCTGAGAATACGCCTGGCCCAGCGCCACCAGAGCTTCACTGTCTTTCGGGTTCGCCCGCACCGCCTGTTGTAGTTCGGGAAATGCCTTTACCCCCCa                                                                   >  2:1741659/1‑140 (MQ=255)                                                                   aGGGCTTTTTCCAGATTCGCCACTGCATTGGCGCGATCGCCTTTCTGAGAATACGCCTGGCCCAGCGCCACCAGAGCTTCACTGTCTTTCGGGTTCGCCCGCACCGCCTGTTGTAGTTCGGG‑AATGGCTTTACCCGCCAt                                                                  >  1:652956/1‑140 (MQ=255)                                                                   aGGGCTTTTTCCAGATTCGCCACTGCATTGGCGCGATCGCCTTTCTGAGAATACGCCTGGCCCAGCGCCACCAGAGCTTCACTGTCTTTCGGGTTCGCCCGCACCGCCTGTTGTAGTTCGGG‑AATGGCTTTACCCGCCAt                                                                  >  1:1458412/1‑140 (MQ=255)                                                                   aGGGCTTTTTCCAGATTCGCCACTGCATTGGCGCGATCGCCTTTCTGAGAATACGCCTGGCCCAGCGCCACCAGAGCTTCACTGTCTTTCGGGTTCGCCCGCACCGCCTGTTGTAGTTCGGG‑AATGGCTTTACCCGCCAt                                                                  >  1:26972/1‑140 (MQ=255)                                                                   aGGGCTTTTTCCAGATTCGCCACTGCATTGGCGCGATCGCCTTTCTGAGAATACGCCTGGCCCAGCGCCACCAGAGCTTCACTGTCTTTCGGGTTCGCCCGCACCGCCTGTTGTAGTTCGGG‑AATGGCTTTACCCGCCAt                                                                  >  1:2059529/1‑140 (MQ=255)                                                                     ggCTTTTTCCAGATTCGCCACTGCATTGGCGCGATCGCCTTTCTGAGAATACGCCTAGCCCAGCGCCACCAGAGCTTCACTGTCTTTCGGGTTCGCCCGCACCGCCTGTTGTAGTTCGGG‑AATGGCTTTACCCGCCATAc                                                                >  2:1631096/1‑140 (MQ=255)                                                                         ttttCCAGATTCGCCACTGCATTGGCGCGATCGCCTTTCTGAGAATACGCCTGGCCCAGCGCCACCAGAGCTTCACTGTCTTTCGGGTTCGCCCGCACCGCCTGTTGTAGTTCGGG‑AATGGCTTTACCCGCCATACCaga                                                            >  1:1267878/1‑140 (MQ=255)                                                                               aGATTCGCCACTGCATTGGCGCGATCGCCTTTCTGAGAATACGCCTGGCCCAGCGCCACCAGAGCTTCACTGTCTTTCGGGTTCGCCCGCACCGCCTGTTGTAGTTCGGG‑AATGGCTTTACCCGCCATACCAGAGTCCAc                                                      >  2:112152/1‑140 (MQ=255)                                                                                 aTTCGCCACTGCATTGGCGCGATCGCCTTTCTGAGAATACGCCTGGCCCAGCGCCACCAGAGCTTCACTGTCTTTCGGGTTCGCCCGCACCGCCTGTTGTAGTTCGGG‑AATGGCTTTACCCGCCATACCAGAGTCCAccg                                                    >  1:501300/1‑140 (MQ=255)                                                                                      ccACTGCATTGGCGCGATCGCCTTTCTGAGAATACGCCTGGCCCAGCGCCACCAGAGCTTCACTGTCTTTCGGGTTCGCCCGCACCGCCTGTTGTAGTTCGGG‑AATGGCTTTACCCGCCATACCAGAGTCCACCGCCGCt                                               >  1:1066277/1‑140 (MQ=255)                                                                                      ccACTGCATTGGCGCGATCGCCTTTCTGAGAATACCCCTGGCCCAGCGCCACCAGAGCTTCACTGTCTTTCGGGGTCGCCCGCACCGCCTGTTGTAGTTCGGC‑AATGGCTTTACCCGCCATACCAGAGTCCACcgccgcc                                               >  2:657816/1‑139 (MQ=255)                                                                                           gCATTGGCGCGATCGCCTTTCTGAGAATACGCCTGGCCCAGCGCCACCAGAGCTTCACTGTCTTTCGGGTTCGCCCGCACCGCCTGTTGTAGTTCGGG‑AATGGCTTTACCCGCCATACCAGAGTCCACCGCCGCTAAAcc                                          >  1:504524/1‑140 (MQ=255)                                                                                                 gcgcGATCGCCTTTCTGAGAATACGCCTGGCCCAGCGCCACCAGAGCTTCACTGTCTTTCGGGTTCGCCCGCACCGCCTGTTGTAGTTCGGG‑AATGGCTTTACCCGCCATACCAGAGTCCACCGCCGCTAAACCTTgcgc                                    >  2:520259/1‑140 (MQ=255)                                                                                                    cgATCGCCTTTCTGAGAATACGCCTGGCCCAGCGCCGCCAGAGCTTCACTGTCTTTCGGGTTCGCCCGCACCGCCTGTTGTAGTTCGGG‑AATGGCTTTACCCGCCATACCAGAGTCCACCGCCGCTAAACCTTGTGCACg                                 >  1:2083242/1‑140 (MQ=255)                                                                                                    cgATCGCCTTTCTGAGAATACGCCTGGCCCAGCGCCACCAGAGCTTCACTGTCTTTCGGGTTCGCCCGCACCGCCTGTTGTAGTTCGGG‑AATGGCTTTACCCGCCATACCAGAGTCCACCGCCGCTAAACCTTGCGCACg                                 >  1:2195837/1‑140 (MQ=255)                                                                                                      aTCGCCTTTCTGAGAATACGCCTGGCCCAGCGCCACCAGAGCTTCACTGTCTTTCGGGTTCGCCCGCACCGCCTGTTGTAGTTCGGG‑AATGGCTTTACCCGCCATACCAGAGTCCACCGCCGCTAAACCTTGCGCACGAg                               >  1:745027/1‑140 (MQ=255)                                                                                                      aTCGCCTTTCTGAGAATACGCCTGGCCCAGCGCCACCAGAGCTTCACTGTCTTTCGGGTTCGCCCGCACCGCCTGTTGTAGTTCGGG‑AATGGCTTTACCCGCCATACCAGAGTCCACCGCCGCTAAACCTTGCGCACGAg                               >  1:185284/1‑140 (MQ=255)                                                                                                        cGCCTTTCTGAGAATACGCCTGGCCCAGCGCCACCAGAGCTTCACTGTCTTTCGGGTTCGCCCGCACCGCCTGTTGTAGTTCGGG‑AATGGCTTTACCCGCCATACCAGAGTCCACCGCCGCTAAACCTTGCGCACGAgcg                             >  1:992716/1‑140 (MQ=255)                                                                                                                     aTACGCCTGGCCCAGCGCCACCAGAGCTTCACTGTCTTTCGGGTTCGCCCGCACCGCCTGTTGTAGTTCGGG‑AATGGCTTTACCCGCCATACCAGAGTCCACCGCCGCTAAACCTTGCGCACGAGCGCGGAAAGCAGGAt                >  2:489946/1‑140 (MQ=255)                                                                                                                     aTACGCCTGGCCCAGCGCCACCAGAGCTTCACTGTCTTTCGGGTTCGCCCGCACCGCCTGTTGTAGTTCGGG‑AATGGCTTTACCCGCCATACCAGAGTCCACCGCCGCTAAACCTTGCGCACGAGCGCGGAAAGCAGGAt                >  1:1761837/1‑140 (MQ=255)                                                                                                                         gCCTGGCCCAGCGCCACCAGAGCTTCACTGTCTTTCGGGTTCGCCCGCACCGCCTGTTGTAGTTCGGG‑AATGGCTTTACCCGCCATACCAGAGTCCACCGCCGCTAAACCTTGCGCACGAGCGCGGAAAGCAGGATCGGc            >  1:512669/1‑140 (MQ=255)                                                                                                                              gCCCAGCGCCACCAGAGCTTCACTGTCTTTCGGGTTCGCCCGCACCGCCTGTTGTAGTTCGGG‑AATGGCTTTACCCGCCATACCAGAGTCCACCGCCGCTAAACCTTGCGCACGAGCGCGGAAAGCAGGATCGGCCAGCt       >  1:496830/1‑140 (MQ=255)                                                                                                                              gCCCAGCGCCACCAGAGCTTCACTGTCTTTCGGGTTCGCCCGCACCGCCTGTTGTAGTTCGGG‑AATGGCTTTACCCGCCATACCAGAGTCCACCGCCGATAAACCTTGCGCACGAGCGCGGAAAGCAGGATCGGCCAGCt       >  1:1931752/1‑140 (MQ=255)                                                                                                                                   gcgcCACCAGAGCTTCACTGTCTTTCGGGTTCGCCCGCACCGCCTGTTGTAGTTCGGG‑AATGGCTTTACCCGCCATACCAGAGTCCACCGCCGCTAAACCTTGCGCACGAGCGCGGAAAGCAGGATCGGCCAGCTGtttt  >  1:1541089/1‑140 (MQ=255)                                                                                                                                        |                                                                                                                                        CAGTAGCGGTTTACTTTCAGCAGACTGTTCCATTTGTCGTTGTTGCTGCTGTGCGGGTCCAGTGCGAGGGCTTTTTCCAGATTCGCCACTGCATTGGCGCGATCGCCTTTCTGAGAATACGCCTGGCCCAGCGCCCCCAGAGCTTCACTGTCTTTCGGGTTCGCCCGCACCGCCTGTTGTAGTTCGGG‑AATGGCTTTACCCGCCATACCAGAGTCCACCGCCGCTAAACCTTGCGCACGAGCGCGGAAAGCAGGATCGGCCAGCTGTTTT  >  NC_000913/3688119‑3688388

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑