BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,345,861	G→T	A489E (GCG→GAG)	fumB ←	fumarase B

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,345,861	0	G	T	99.2%	441.7 / NA	128	A489E (GCG→GAG)	fumB	fumarase B
Reads supporting (aligned to +/- strand): ref base G (1/0); major base T (75/51); minor base C (0/1); total (76/52)
Fisher's exact test for biased strand distribution p-value = 4.09e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 143 total aligned reads displayed.

AGAAGTCGTTACCTTTGTCATCGACCAGGATAAACGCCGGGAAATCTTCTACTTCGATTTTCCAGATAGCTTCCATACCCAGCTCCGGATAAGCGACGCACTCCAGATGCTTGATGCTCTGCTGCGCCAGTACCGCCGCCGGACCGCCGATGCTACCGAGGTAGAAGCCGCCGTGTTTATGACACGCGTCGGTAACCTGCTGACTGCGGTTACCTTTCGCCAGCATGATCATGCTGCCGCCGTGGGATTGCAGCAGATCCACGTAGGAG  >  NC_000913/4345724‑4345992

agaagTCGTTACCTTTGTCATCGACCAGGATAAACGCCGGGAAATCTTCTACTTCGATTTTCCAGATAGCTTCCATACCCAGCTCCGGATAAGCGACGCACTCCAGATGCTTGATGCTCTGCTGCGCCAGTAccgcctcc                                                                                                                                   >  1:164775/1‑140 (MQ=255)

       gTTACCTTTGTCATCGACCAGGATAAACGCCGGGAAATCTTCTACTTCGATTTTCCAGATAGCTTCCATACCCAGCTCCGGATAAGCGACGCACTCCAGATGCTTGATGCTCTGCTGCGCCAGTACCGCCTCCGGAccgc                                                                                                                            <  2:1306555/140‑1 (MQ=255)

       gTTACCTTTGTCATCGACCAGGATAAACGCCGGGAAATCTTCTACTTCGATTTTCCAGATAGCTTCCATACCCAGCTCCGGATAAGCGACGCACTCCAGATGCTTGATGCTCTGCTGCGCCAGTACCGCCTCCGGAccgc                                                                                                                            <  2:311283/140‑1 (MQ=255)

        ttACCTTTGTCATCGACCAGGATAAACGCCGGGAAATCTTCTACTTCGATTTTCCAGATAGCTTCCATACCCAGCTCCGGATAAGCGACGCACTCCAGATGCTTGATGCTCTGCTGCGCCAGTACCGCCTCCGGAccgcc                                                                                                                           <  1:925828/140‑1 (MQ=255)

          cccTTTGTCATCGACCAGGATAAACGCCGGGAAATCTTCTACTTCGATTTTCCAGATAGCTTCCATACCCAGCTCCGGATAAGCGACGCACTCCAGATGCTTGATGCTCTGCTGCGCCAGTACCGCCTCCGGACCGCCGa                                                                                                                         <  2:1647939/139‑1 (MQ=255)

                   aTCGACCAGGATAAACGCCGGGAAATCTTCTACTTCGATTTTCCAGATAGCTTCCATACCCAGCTCCGGATAAGCGACGCACTCCAGATGCTTGATGCTCTGCTGCGCCAGTACCGCCTCCGGACCGCCGATGCTACCGa                                                                                                                >  1:2139977/1‑140 (MQ=255)

                   aTCGACCAGGATAAACGCCGGGAAATCTTCTACTTCGATTTTCCAGATAGCTTCCATACCCAGCTCCGGATAAGCGACGCACTCCAGATGCTTGATGCTCTGCTGCGCCAGTACCGCCTCCGGACCGCCGATGCTACCGa                                                                                                                >  2:1529601/1‑140 (MQ=255)

                   aTCGACCAGGATAAACGCCGGGAAATCTTCTACTTCGATTTTCCAGATAGCTTCCATACCCAGCTCCGGATAAGCGACGCACTCCAGATGCTTGATGCTCTGCTGCGCCAGTACCGCCTCCGGACCGCCGATGCTACCGa                                                                                                                >  2:1659689/1‑140 (MQ=255)

                   aTCGACCAGGATAAACGCCGGGAAATCTTCTACTTCGATTTTCCAGATAGCTTCCATACCCAGCTCCGGATAAGCGACGCACTCCAGATGCTTGATGCTCTGCTGCGCCAGTACCGCCTCCGGACCGCCGATGCTACCGa                                                                                                                >  1:1449476/1‑140 (MQ=255)

                      gACCAGGATAAACGCCGGGAAATCTTCTACTTCGATTTTCCAGATAGCTTCCATACCCAGCTCCGGATAAGCGACGCACTCCAGATGCTTGATGCTCTGCTGCGCCAGTACCGCCTCCGGACCGCCGATGCTACCGAGGt                                                                                                             <  2:927383/140‑1 (MQ=255)

                                aaCGCCGGGAAATCTTCTACTTCGATTTTCCAGATAGCTTCCATACCCAGCTCCGGATAAGCGACGCACTCCAGATGCTTGATGCTCTGCTGCGCCAGTACCGCCTCCGGACCGCCGATGCTACCGAGGTAGAAgccgcc                                                                                                   <  2:176574/140‑1 (MQ=255)

                                 aCGCCGGGAAATCTTCTACTTCGATTTTCCAGATAGCTTCCATACCCAGCTCCGGATAAGCGACGCACTCCAGATGCTTGATGCTCTGCTGCGCCAGTACCGCCTCCGGACCGCCGATGCTACCGAGGTAGAAGccgccg                                                                                                  >  1:1890672/1‑140 (MQ=255)

                                 aCGCCGGGAAATCTTCTACTTCGATTTTCCAGATAGCTTCCATACCCAGCTCCGGATAAGCGACGCACTCCAGATGCTTGATGCTCTGCTGCGCCAGTACCGCCTCCGGACCGCCGATGCTACCGAGGTAGAAGccgccg                                                                                                  >  2:1114611/1‑140 (MQ=255)

                                   gCCGGGAAATCTTCTACTTCGATTTTCCAGATAGCTTCCATACCCAGCTCCGGATAAACGACGCACTCCAGATGCTTGATGCTCTGCTGCGCCAGTACCGCCTCCGGACCGCCGATGCTACCGAGGTAGAAGCCGCCgtg                                                                                                >  1:552410/1‑140 (MQ=255)

                                     cGGGAAATCTTCTACTTCGATTTTCCAGATAGCTTCCATACCCAGCTCCGGATAAGCGACGCACTCCAGATGCTTGATGCTCTGCTGCGCCAGTACCGCCTCCGGACCGCCGATGCTACCGAGGTAGAAGCCGCCGTGtt                                                                                              >  1:1238563/1‑140 (MQ=255)

                                      gggAAATCTTCTACTTCGATTTTCCAGATAGCTTCCATACCCAGCTCCGGATAAGCGACGCACTCCAGATGCTTGATGCTCTGCTGCGCCAGTACCGCCTCCGGACCGCCGATGCTACCGAGGTAGAAGCCGCCGTGttt                                                                                             >  1:1602880/1‑140 (MQ=255)

                                      gggAAATCTTCTACTTCGATTTTCCAGATAGCTTCCATACCCAGCTCCGGATAAGCGACGCACTCCAGATGCTTGATGCTCTGCTGCGCCAGTACCGCCTCCGGACCGCCGATGCTACCGAGGTAGAAGCCGCCGTGttt                                                                                             >  2:1928304/1‑140 (MQ=255)

                                       ggAAATCTTCTACTTCGATTTTCCAGATAGCTTCCATACCCAGCTCCGGATAAGCGACGCACTCCAGATGCTTGATGCTCTGCTGCGCCAGTACCGCCTCCGGACCGCCGATGCTACCGAGGTAGAAGCCGCCGTGTTTa                                                                                            >  2:1503380/1‑140 (MQ=255)

                                       ggAAATCTTCTACTTCGATTTTCCAGATAGCTTCCATACCCAGCTCCGGATAAGCGACGCACTCCAGATGCTTGATGCTCTGCTGCGCCAGTACCGCCTCCGGACCGCCGATGCTACCGAGGCAGAAGCCGCCGTGTTTa                                                                                            >  1:723640/1‑140 (MQ=255)

                                       ggAAATCTTCTACTTCGATTTTCCAGATAGCTTCCATACCCAGCTCCGGATAAGCGACGCACTCCAGATGCTTGATGCTCCGCTGCGCCAGTACCGCCTCCGGACCGCCGATGCTACCGAGGTAGAAGCCGCCGTGTTTa                                                                                            >  1:1390678/1‑140 (MQ=255)

                                            tcttctACTTCGATTTTCCAGATAGCTTCCATACCCAGCTCCGGATAAGCGACGCACTCCAGATGCTTGATGCTCTGCTGCGCCAGTACCGCCTCCGGACCGCCGATGCTACCGAGGTAGAAGCCGCCGTGTTTATGaca                                                                                       <  2:1098424/140‑1 (MQ=255)

                                              ttctACTTCGATTTTCCAGATAGCTTCCATACCCAGCTCCGGATAAGCGACGCACTCCAGATGCTTGATGCTCTGCTGCGCCAGTACCGCCTCCGGACCGCCGATGCTACCGAGGTAGAAGCCGCCGTGTTTATGACAcg                                                                                     <  1:2030869/140‑1 (MQ=255)

                                              ttctACTTCGATTTTCCAGATAGCTTCCATACCCAGCTCCGGATAAGCGACGCACTCCAGATGCTTGATGCTCTGCTGCGCCAGTACCGCCTCCGGACCGCCGATGCTACCGAGGTAGAAGCCGCCGTGTTTATGACAcg                                                                                     <  2:1180941/140‑1 (MQ=255)

                                                   cTTCGATTTTCCAGATAGCTTCCATACCCAACTCCGGATAAGCGACGCACTCCAGATGCTTGATGCTCTGCTGCGCCAGTACCGCCTCCGGACCGCCGATGCTACCGAGGTAGAAGCCGCCGTGTTTATGACACGCGTCg                                                                                >  2:437365/1‑140 (MQ=255)

                                                     tCGATTTTCCAGATAGCTTCCATACCCAGCTCCGGATAAGCGACGCACTCCAGATGCTTGATGCTCTGCTGCGCCAGTACCGCCTCCGGACCGCCGATGCTACCGAGGTAGAAGCCGCCGTGTTTATGACACGCGTCGGt                                                                              <  1:432569/140‑1 (MQ=255)

                                                      cGATTTTCCAGATAGCTTCCATACCCAGCTCCGGATAAGCGACGCACTCCAGATGCTTGATGCTCTGCTGCGCCAGTACCGCCTCCGGACCGCCGATGCTACCGAGGTAGAAGCCGCCGTGTTTATGACACGCGTCGGTa                                                                             >  1:1549602/1‑140 (MQ=255)

                                                       gATTTTCCAGATAGCTTCCATACCCAGCTCCGGATAAGCGACGCACTCCAGATGCTTGATGCTCTGCTGCGCCAGTACCGCCTCCGGACCGCCGATGCTACCGAGGTAGAAGCCGCCGTGTTTATGACACGCGTCGGTaa                                                                            >  1:1046218/1‑140 (MQ=255)

                                                              cAGATAGCTTCCATACCCAGCTCCGGATAAGCGACGCACTCCAGATGCTTGATGCTCTGCTGCGCCAGTACCGCCTCCGGACCGCCGATGCTACCGAGGTAGAAGCCGCCGTGTTTATGACACGCGTCGGTAACctgctg                                                                     <  1:869156/140‑1 (MQ=255)

                                                                  tAGCTTCCATACCCAGCTCCGGATAAGCGACGCACTCCAGATGCTTGATGCTCTGCTGCGCCAGTACCGCCTCCGGACCGCCGATGCTACCGAGGTAGAAGCCGCCGTGTTTATGACACGCGTCGGTAACCTGCTGACTg                                                                 <  1:191088/140‑1 (MQ=255)

                                                                     cTTCCATACCCAGCTCCGGATAAGCGACGCACTCCAGATGCTTGATGCTCTGCTGCGCCAGTACCGCCTCCGGACCGCCGATGCTACCGAGGTAGAAGCCGCCGTGTTTATGACACGCGTCGGTAACCTGCTGACTGCgg                                                              >  1:1690005/1‑140 (MQ=255)

                                                                      ttCCATACCCAGCTCCGGATAAGCGACGCACTCCAGATGCTTGATGCTCTGCTGCGCCAGTACCGCCTCCGGACCGCCGATGCTACCGAGGTAGAAGCCGCCGTGTTTATGACACGCGTCGGTAACCTGCTGACTGCGGt                                                             <  2:1931637/140‑1 (MQ=255)

                                                                      ttCCATACCCAGCTCCGGATAAGCGACGCACTCCAGATGCTTGATGCTCTGCTGCGCCAGTACCGCCTCCGGACCGCCGATGCTACCGAGGTAGAAGCCGCCGTGTTTATGACACGCGTCGGTAACCTGCTGACTGCGGt                                                             >  1:1663527/1‑140 (MQ=255)

                                                                          aTACCCAGCTCCGGATAAGCGACGCACTCCAGATGCTTGATGCTCTGCTGCGCCAGTACCGCCTCCGGACCGCCGATGCTACCGAGGTAGAAGCCGCCGTGTTTATGACACGCGTCGGTAACCTGCTGACTGCGGTTAcc                                                         >  1:1575663/1‑140 (MQ=255)

                                                                           tACCCAGCTCCGGATAAGCGACGCACTCCAGATGCTTGATGCTCTGCTGCGCCAGTACCGCCTCCGGACCGCCGATGCTACCGAGGTAGAAGCCGCCGTGTTTATGACACGCGTCGGTAACCTGCTGACTGCGGTTACCt                                                        >  2:655571/1‑140 (MQ=255)

                                                                           tACCCAGCTCCGGATAAGCGACGCACTCCAGATGCTTGATGCTCTGCTGCGCCAGTACCGCCTCCGGACCGCCGATGCTACCGAGGTAGAAGCCGCCGTGTTTATGACACGCGTCGGTAACCTGCTGACTGCGGTTACCt                                                        >  1:2193690/1‑140 (MQ=255)

                                                                              ccAGCTCCGGATAAGCGACGCACTCCAGATGCTTGATGCTCTGCTGCGCCAGTACCGCCTCCGGACCGCCGATGCTACCGAGGTAGAAGCCGCCGTGTTTATGACACGCGTCGGTAACCTGCTGACTGCGGTTACCTTTc                                                     <  1:1886820/140‑1 (MQ=255)

                                                                               cAGCTCCGGATAAGCGACGCACTCCAGATGCTTGATGCTCTGCTGCGCCAGTACCGCCTCCGGACCGCCGATGCTACCGAGGTAGAAGCCGCCGTGTTTATGACACGCGTCGGTAACCTGCTGACTGCGGTTACCTTTCg                                                    <  1:1309729/140‑1 (MQ=255)

                                                                               cAGCTCCGGATAAGCGACGCACTCCAGATGCTTGATGCTCTGCTGCGCCAGTACCGCCTCCGGACCGCCGATGCTACCGAGGTAGAAGCCGCCGTGTTTATGACACGCGTCGGTAACCTGCTGACTGCGGTTACCTTTCg                                                    <  1:693199/140‑1 (MQ=255)

                                                                               cAGCTCCGGATAAGCGACGCACTCCAGATGCTTGATGCTCTGCTGCGCCAGTACCGCCTCCGGACCGCCGATGCTACCGAGGTAGAAGCCGCCGTGTTTATGACACGCGTCGGTAACCTGCTGACTGCGGTTACCTTTCg                                                    <  2:1690005/140‑1 (MQ=255)

                                                                               cAGCTCCGGATAAGCGACGCACTCCAGATGCTTGATGCTCTGCTGCGCCAGTACCGCCTCCGGACCGCCGATGCTACCGAGGTAGAAGCCGCCGTGTTTATGACACGCGTCGGTAACCTGCTGACTGCGGTTACCTTTCg                                                    >  2:1704390/1‑140 (MQ=255)

                                                                               cAGCTCCGGATAAGCGACGCACTCCAGATGCTTGATGCTCTGCTGCGCCAGTACCGCCTCCGGACCGCCGATGCTACCGAGGTAGAAGCCGCCGTGTTTATGACACGCGTCGGTAACCTGCTGACTGCGGTTACCTTTCg                                                    >  2:2069669/1‑140 (MQ=255)

                                                                               cAGCTCCGGATAAGCGACGCACTCAAGATCCTTGATGCTCTGCTGCGCCAGTACCGCCCCCGGACCGCCGATGCTACCGAGGTAGAAGCCGCCGTGTTTATGACACGCGTCGGTAACCTGCTGACTGCGGTTACCTTTCg                                                    <  1:1149811/140‑1 (MQ=255)

                                                                                aGCTCCGGATAAGCGACGCACTCCAGATGCTTGATGCTCTGCTGCGCCAGTACCGCCTCCGGACCGCCGATGCTACCGAGGTAGAAGCCGCCGTGTTTATGACACGCGTCGGTAACCTGCTGACTGCGGTTACCTTTCGc                                                   >  2:986455/1‑140 (MQ=255)

                                                                                  cTCCGGATAAGCGACGCACTCCAGATGCTTGATGCTCTGCTGCGCCAGTACCGCCTCCGGACCGCCGATGCTACCGAGGTAGAAGCCGCCGTGTTTATGACACGTGTCGGTAACCTGCTGAc                                                                   <  2:1742165/122‑1 (MQ=255)

                                                                                  cTCCGGATAAGCGACGCACTCCAGATGCTTGATGCTCTGCTGCGCCAGTACCGCCTCCGGACCGCCGATGCTACCGAGGTAGAAGCCGCCGTGTTTATGACACGTGTCGGTAACCTGCTGAc                                                                   >  1:1742165/1‑122 (MQ=255)

                                                                                  cTCCGGATAAGCGACGCACTCCAGATGCTTGATGCTCTGCTGCGCCAGTACCGCCTCCGGACCGCCGATGCTACCGAGGTAGAAGCCGCCGTGTTTATGACACGCGTCGGTAACCTGCTGACTGCGGTTACCTTTCGCCa                                                 >  2:596561/1‑140 (MQ=255)

                                                                                  cTCCGGATAAGCGACGCACTCCAGATGCTTGATGCTCTGCTGCGCCAGTACCGCCTCCGGACCGCCGATGCTACCGAGGTAGAAGCCGCCGTGTTTATGACACGCGTCGGTAACCTGCTGACTGCGGTTACCTTTCGCCa                                                 >  2:1835101/1‑140 (MQ=255)

                                                                                  cTCCGGATAAGCGACGCACTCCAGATGCTTGATGCTCTGCTGCGCCAGTACCGCCTCCGGACCGCCGATGCTACCGAGGTAGAAGCCGCCGTGTTTATGACACGCGTCGGTAACCTGCTGACTGCGGTTACCTTTCGCCa                                                 >  1:1358673/1‑140 (MQ=255)

                                                                                  cTCCGGATAAGCGACGCACTCCAGATGCTTGATGCTCTGCTGCGCCAGTACCGCCTCCGGACCGCCGATGCTACCGAGGTAGAAGCCGCCGTGTTTATGACACGCGTCGGTAACCTGCTGACTGCGGTTACCTTTCGCCa                                                 >  2:513898/1‑140 (MQ=255)

                                                                                  cTCCGGATAAGCGACGCACTCCAGATGCTTGATGCTCTGCTGCGCCAGTACCGCCTCCGGACCGCCGATGCTACCGAGGTAGAAGCCGCCGTGTTTATGACACGCGTCGGTAACCTGCTGACTGCGGTTACCTTTCGCCa                                                 >  2:29060/1‑140 (MQ=255)

                                                                                  cTCCGGATAAGCGACGCACTCCAGATGCTTGATGCTCTGCTGCGCCAGTACCGCCTCCGGACCGCCGATGCTACCGAGGTAGAAGCCGCCGTGTTTATGACACGCGTCGGTAACCTGCTGACTGCGGTTACCTTTCGCCa                                                 >  2:1686160/1‑140 (MQ=255)

                                                                                  cTCCGGATAAGCGACGCACTCCAGATGCTTGATGCTCTGCTGCGCCAGTACCGCCTCCGGACCGCCGATGCTACCGAGGTAGAAGCCGCCGTGTTTATGACACGCGTCGGTAACCTGCTGACTGCGGTTACCTTTCGCCa                                                 >  1:1678724/1‑140 (MQ=255)

                                                                                  cTCCGGATAAGCGACGCACTCCAGATGCTTGATGCTCTGCTGCGCCAGTACCGCCTCCGGACCGCCGATGCTACCGAGGTAGAAACCGCCGTGTTTATGACACGCGTCGGTAACCTGCTGACTGCGGTTACCTTTCGCCa                                                 >  2:1461084/1‑140 (MQ=255)

                                                                                  cTCCGGATAAGCGACGCACTCCAGATGCTTGATGCTCTGCTGCGCCAGTACCGCCTCCGAACCGCCGATGCTACCGAGGTAGAAGCCGCCGTGTTTATGACACGCGTCGGTAACCTGCTGACTGCGGTTACCTTTCGCCa                                                 >  1:1982720/1‑140 (MQ=255)

                                                                                  cTCCGGATAAGCGACCCACTCCAGATGCTTGATGCTCTGCTGCGCCAGTACCGCCTCCGAATCGTCGATGCTACCGAGGTAGAAGCCGCCGTGTTTATGACTCGCGTCGGTAACCTGCTGACTGCGGTTACCTTTCACCa                                                 >  2:842782/1‑140 (MQ=38)

                                                                                   tCCGGATAAGCGACGCACTCCAGATGCTTGATGCTCTGCTGCGCCAGTACCGCCTCCGGACCGCCGATGCTACCGAGGTAGAAGCCGCCGTGTTTATGACACGCGTCGGTAACCTGCTGACTGCGGTTACCTTTCGCCAg                                                >  1:831216/1‑140 (MQ=255)

                                                                                   cccGGATAAGCGCCGCCCTCCAGATGCTTGAGGCTCCGCTGCGCCAGTCCCGCCTCCGGACCGCCGATGCTACCGAGGTAGAAGCCGCCGTGTTTATGACACGCGTCGGTAACCTGCTGACTGCGGTTACCTTTCGCCAg                                                <  2:1390678/139‑1 (MQ=37)

                                                                                     cGGATAAGCGACGCACTCCAGATGCTTGATGCTCTGCTGCGCCAGTACCGCCTCCGGACCGCCGATGCTACCGAGGTAGAAGCCGCCGTGTTTATGACACGCGTCGGTAACCTGCTGACTGCGGTTACCTTTCGcc                                                  >  1:1095202/1‑136 (MQ=255)

                                                                                     cGGATAAGCGACGCACTCCAGATGCTTGATGCTCTGCTGCGCCAGTACCGCCTCCGGACCGCCGATGCTACCGAGGTAGAAGCCGCCGTGTTTATGACACGCGTCGGTAACCTGCTGACTGCGGTTACCTTTCGcc                                                  <  2:1095202/136‑1 (MQ=255)

                                                                                     cGGATAAGCGACGCACTCCAGATGCTTGATGCTCTGCTGCGCCAGTACCGCCTCCGGACCGCCGATGCTACCGAGGTAGAAGCCGCCGTGTTTATGACACGCGTCGGTAACCTGCTGACTGCGGTTACCTTTCGCCAGCa                                              >  1:109643/1‑140 (MQ=255)

                                                                                         tAAGCGACGCACTCCAGATGCTTGATGCTCTGCTGCGCCAGTCCCGCCTCCGGACCGCCGATGCTACCGAGGTAGAAGCCGCCGTGTTTATGACACGCGTCGGTAACCTGCTGACTGCGGTTACCTTTCGCCAGCATGAt                                          <  2:1133730/140‑1 (MQ=255)

                                                                                         tAAGCGACGCACTCCAGATGCTTGATGCTCTGCTGCGCCAGTACCGCCTCCGGACCGCCGATGCTACCGAGGTAGAAGCCGCCGTGTTTATGACACGCGTCGGTAACCTGCTGACTGCGGTTACCTTTCGCCAGCATGAt                                          <  1:1676227/140‑1 (MQ=255)

                                                                                          aaGCGACGCACTCCAGATGCTTGATGCTCTGCTGCGCCAGTACCGCCTCCGGACCGCCGATGCTACCGAGGTAGAAGCCGCCGTGTTTATGACACGCGTCGGTAACCTGCTGACTGCGGTTACCTTTCGCCAGCATGATc                                         <  1:1503380/140‑1 (MQ=255)

                                                                                            gCGACGCACTCCAGATGCTTGATGCTCTGCTGCGCCAGTACCGCCTCCGGACCGCCGATGCTACCGAGGTAGAAGCCGCCGTGTTTATGACACGCGTCGGTAACCTGCTGACTGCGGTTACCTTTCGCCAGCATGATCAt                                       >  2:1432464/1‑140 (MQ=255)

                                                                                             cGACGCACTCCAGATGCTTGATGCTCTGCTGCGCCAGTACCGCCTCCGGACCGCCGATGCTACCGAGGTAGAAGCCGCCGTGTTTATGACACGCGTCGGTAACCTGCTGACTGCGGTTACCTTTCGCCAGCATGATCAtg                                      >  2:2060345/1‑140 (MQ=255)

                                                                                               aCGCACTCCAGATGCTTGATGCTCTGCTGCGCCAGTACCGCCTCCGGACCGCCGATGCTACCGAGGTAGAAGCCGCCGTGTTTATGACACGCGTCGGTAACCTGCTGACTGCGGTTACCTTTCGCCAGCATGATCAtgct                                    >  1:1568121/1‑140 (MQ=255)

                                                                                                cccccTCAAGATGTTTGATGCTTTGCTCCGCAAGTCCCGCCCCCGGACCGCGGATGCTACCGAGGTAGAAGCCGCCGTGTTTATGACACGCGTCGGTAACCTGCTGACTGCGGTTACCTTTCGCCAGCATGATCAtgctg                                   <  2:98535/136‑1 (MQ=25)

                                                                                                cGCACTCCAGATGCTTGATGCTCTGCTGCGCCAGTACCGCCTCCGGACCGCCGATGCTACCGAGGTAGAAGCCGCCGTGTTTATGACACGCGTCGGTAACCTGCTGACTGCGGTTACCTTTCGCCAGCATGATCAtgctg                                   >  2:1605345/1‑140 (MQ=255)

                                                                                                 gCACTCCAGATGCTTGATGCTCTGCTGCGCCAGTACCGCCTCCGGACCGCCGATGCTACCGAGGTAGAAGCCGCCGTGTTTATGACACGCGTCGGTAACCTGCTGACTGCGGTTACCTTTCGCCAGCATGATCAtgctgc                                  >  1:2214095/1‑140 (MQ=255)

                                                                                                    cTCCAGATGCTTGATGCTCTGCTGCGCCAGTACCGCCTCCGGACCGCCGATGCTACCGAGGTAGAAGCCGCCGTGTTTATGACACGCGTCGGTAACCTGCTGACTGCGGTTACCTTTCGCCAGCATGATCATGCTgccgc                               <  1:1605345/140‑1 (MQ=255)

                                                                                                      ccAGATGCTTGATGCTCTGCTGCGCCAGTACCGCCTCCGGACCGCCGATGCTACCGAGGTAGAAGCCGCCGTGTTTATGACACGCGTCGGTAACCTGCTGACTGCGGTTACCTTTCGCCAGCATGATCATGCTGccgccg                             <  1:879365/140‑1 (MQ=255)

                                                                                                       cAGATGCTTGATGCTCTGCTGCGCCAGTACCGCCTCCGGACCGCCGATGCTACCGAGGTAGAAGCCGCCGTGTTTATGACACGCGTCGGTAACCTGCTGACTGCGGTTACCTTTCGCCAGCATGATCATGCTGCCGCCGt                            <  2:831216/140‑1 (MQ=255)

                                                                                                       cAGATGCTTGATGCTCTGCTGCGCCAGTACCGCCTCCGGACCGCCGATGCTACCGAGGTAGAAGCCGCCGTGTTTATGACACGCGTCGGTAACCTGCTGACTGCGGTTACCTTTCGCCAGCATGATCATGCTGCCGCCGt                            >  1:365749/1‑140 (MQ=255)

                                                                                                          aTGCTTGATGCTCTGCTGCGCCAGTACCGCCTCCGGACCGCCGATGCTACCGAGGTAGAAGCCGCCGTGTTTATGACACGCGTCGGTAACCTGCTGACTGCGGTTACCTTTCGCCAGCATGATCATGCTGCCGCCGTggg                         >  2:161489/1‑140 (MQ=255)

                                                                                                            gCTTGTTCCTCTGCTGCGCCAGTCCCGCCCCCGGACCGCCGATGCTACCGAGGTAGAAGCCGCCGTGTTTATGACACGCGTCGGTAACCTGCTGACTGCGGTTACCTTTCGCCAGCATGATCATGCTGCCGCCGTGGGAt                       <  2:1046218/140‑1 (MQ=255)

                                                                                                            gCTTGATGCTCTGCTGCGCCAGTCCCGCCTCCGGACCGCCGATGCTACCGAGGTAGAAGCCGCCGTGTTTATGACACGCGTCGGTAACCTGCTGACTGCGGTTACCTTTCGCCAGCATGATCATGCTGCCGCCGTGGGAt                       <  1:1928304/140‑1 (MQ=255)

                                                                                                             cTTGATGCTCTGCTGCGCCAGTACCGCCTCCGGACCGCCGATGCTACCGAGGTAGAAGCCGCCGTGTTTATGACACGCGTCGGTAACCTGCTGACTGCGGTTACCTTTCGCCAGCATGATCATGCTGCCGCCGTGGGAtt                      >  1:1136177/1‑140 (MQ=255)

                                                                                                              ttGATCCTCTGCTGCCCCAGTACCGCCTCCGGACCGCCGATGCTACCGAGGTAGAAGCCGCCGTGTTTATGACACGCGTCGGTAACCTGCTGACTGCGGTTACCTTTCGCCAGCATGATCATGCTGCCGCCGTGGGATTg                     <  1:1639876/140‑1 (MQ=255)

                                                                                                               tGATGCTCTGCTGCGCCAGTACCGCCTCCGGACCGCCGATGCTACCGAGGTAGAAGCCGCCGTGTTTATGACACGCGTCGGTAACCTGCTGACTGCGGTTACCTTTCGCCAGCATGATCATGCTGCCGCCGTGGGATTgc                    >  2:595853/1‑140 (MQ=255)

                                                                                                               tGATGCTCTGCTGCGCCAGTACCGCCTCCGGACCGCCGATGCTACCGAGGTAGAAGCCGCCGTGTTTATGACACGCGTCGGTAACCTGCTGACTGCGGTTACCTTTCGCCAGCATGATCATGCTGCCGCCGTGGGATTgc                    >  1:904738/1‑140 (MQ=255)

                                                                                                               tGATGCTCTGCTGCGCCAGTACCGCCTCCGGACCGCCGATGCTACCGAGGTAGAAGCCGCCGTGTTTATGACACGCGTCGGTAACCTGCTGACTGCGGTTACCTTTCGCCAGCATGATCATGCTGCCGCCGTGGGATTgc                    >  1:1778784/1‑140 (MQ=255)

                                                                                                                 aTGCTCTGCTGCGCCAGTACCGCCTCCGGACCGCCGATGCTACCGAGGTAGAAGCCGCCGTGTTTATGACACGCGTCGGTAACCTGCTGACTGCGGTTACCTTTCGCCAGCATGATCATGCTGCCGCCGTGGGATTgcag                  <  1:437365/140‑1 (MQ=255)

                                                                                                                 aTGCTCTGCTGCGCCAGTACCGCCTCCGGACCGCCGATGCTACCGAGGTAGAAGCCGCCGTGTTTATGACACGCGTCGGTAACCTGCTGACTGCGGTTACCTTTCGCCAGCATGATCATGCTGCCGCCGTGGGATTgcag                  >  1:1857463/1‑140 (MQ=255)

                                                                                                                  tGCTCTGCTGCGCCAGTACCGCCTCCGGACCGCCGATGCTACCGAGGTAGAAGCCGCCGTGTTTATGACACGCGTCGGTAACCTGCTGACTGCGGTTACCTTTCGCCAGCATGATCATGCTGCCGCCGTGGGATTgcagc                 <  1:305458/140‑1 (MQ=255)

                                                                                                                    ctctGCTGCGCCAGTACCGCCTCCGGACCGCCGATGCTACCGAGGTAGAAGCCGCCGTGTTTATGACACGCGTCGGTAACCTGCTGACTGCGGTTACCTTTCGCCAGCATGATCATGCTGCCGCCGTGGGATTGcagcag               >  1:1774923/1‑140 (MQ=255)

                                                                                                                       tgctgcGCCAGTACCGCCTCCGGACCGCCGATGCTACCGAGGTAGAAGCCCCCGTGTTTATGACACGCGTCGGTAACCTGCTGACTGCGGTTACCTTTCGCCAGCATGATCATGCTGCCGCCGTGGGATTGCAGCAGATc            >  2:1594567/1‑140 (MQ=255)

                                                                                                                       tgctgcGCCAGTACCGCCGCCGGACCGCCGATGCTACCGAGGTAGAAGCCGCCGTGTTTATGACACGCGTCGGTAACCTGCTGACTGCGGTTACCTTTCGCCAGCATGATCATGCTGCCGCCGTGGGATTGCAGCAGATc            >  1:884724/1‑140 (MQ=255)

                                                                                                                        gctgcGCCAGTACCGCCTCCGGACCGCCGATGCTACCGAGGTAGAAGCCGCCGTGTTTATGACACGCGTCGGTAACCTGCTGACTGCGGTTACCTTTCGCCAGCATGATCATGCTGCCGCCGTGGGATTGCAGCAGATcc           >  2:2055144/1‑140 (MQ=255)

                                                                                                                        gctgcGCCAGTACCGCCTCCGGACCGCCGATGCTACCGAGGTAGAAGCCGCCGTGTTTATGACACGCGTCGGTAACCTGCTGACTGCGGTTACCTTTCGCCAGCATGATCATGCTGCCGCCGTGGGATTGCAGCAGATcc           >  1:1157402/1‑140 (MQ=255)

                                                                                                                           gcgcCAGTACCGCCTCCGGACCGCCGATGCTACCGAGGTAGAAGCCGCCGTGTTTATGACACGCGTCGGTAACCTGCTGACTGCGGTTACCTTTCGCCAGCATGATCATGCTGCCGCCGTGGGATTGCAGCAGATCCACg        <  1:793911/140‑1 (MQ=255)

                                                                                                                            cgcCAGTACCGCCTCCGGACCGCCGATGCTACCGAGGTAGAAGCCGCCGTGTTTATGACACGCGTCGGTAACCTGCTGACTGCGGTTACCTTTCGCCAGCATGATCATGCTGCCGCCGTGGGATTGCAGCAGATCCACGt       <  1:1224224/140‑1 (MQ=255)

                                                                                                                            cgcCAGTACCGCCTCCGGACCGCCGATGCTACCGAGGTAGAAGCCGCCGTGTTTATGACACGCGTCGGTAACCTGCTGACTGCGGTTACCTTTCGCCAGCATGATCATGCTGCCGCCGTGGGATTGCAGCAGATCCACGt       >  1:981294/1‑140 (MQ=255)

                                                                                                                             gcCATTACCGCCTCCGGACCGCCGATGCTACCGAGGTAGAAGCCGCCGTGTTTATGACACGCGTCGGTAACCTGCTGACTGCGGTTACCTTTCGCCAGCATGATCATGCTGCCGCCGTGGGATTGCAGCAGATCCACGTa      <  1:513898/140‑1 (MQ=255)

                                                                                                                             gcCAGTCCCGCCTCCGGACCGCCGATGCTACCGAGGTAGAAGCCGCCGTGTTTATGACACGCGTCGGTAACCTGCTGACTGCGGTTACCTTTCGCCAGCATGATCATGCTGCCGCCGTGGGATTGCAGCAGATCCACGTa      <  2:1347371/140‑1 (MQ=255)

                                                                                                                             cccAGTCCCGCCTCCGGACCGCCGATGCTACCGAGGTAGAAGCCGCCGTGTTTATGACACGCGTCGGTAACCTGCTGACTGCGGTTACCTTTCGCCAGCATGATCATGCTGCCGCCGTGGGATTGCAGCAGATCCACGTa      <  2:1190542/139‑1 (MQ=255)

                                                                                                                              ccAGTACCGCCTCCGGACCGCCGATGCTACCGAGGTAGAAGCCGCCGTGTTTATGACACGCGTCGGTAACCTGCTGACTGCGGTTACCTTTCGCCAGCATGATCATGCTGCCGCCGTGGGATTGCAGCAGATCCACGTAg     >  2:1833543/1‑140 (MQ=255)

                                                                                                                              ccAGTACCGCCTCCGGACCGCCGATGCTACCGAGGTAGAAGCCGCCGTGTTTATGACACGCGTCGGTAACCTGCTGACTGCGGTTACCTTTCGCCAGCATGATCATGCTGCCGCCGTGGGATTGCAGCAGATCCACGTAg     >  1:57371/1‑140 (MQ=255)

                                                                                                                                ggTACCGCCTCCGGACCGCCGATGCTACCGAGGTAGAAGCCGCCGTGTTTATGACACGCGTCGGTAACCTGCTGACTGCGGTTACCTTTCGCCAGCATGATCATGCTGCCGCCGTGGGATTGCAGCAGATCCACGTAGGa   <  1:963457/139‑1 (MQ=255)

                                                                                                                                aGTACCGCCTCCGGACCGCCGATGCTACCGAGGTAGAAGCCGCCGTGTTTATGACACGCGTCGGTAACCTGCTGACTGCGGTTACCTTTCGCCAGCATGATCATGCTGCCGCCGTGGGATTGCAGCAGATCCACGTAGGa   <  1:1114611/140‑1 (MQ=255)

                                                                                                                                 gTACCGCCGCCGGACCGCCGATGCTACCGAGGTAGAAGCCGCCGTGTTTATGACACGCGTCGGTAACCTGCTGACTGCGGTTACCTTTCGCCAGCATGATCATGCTGCCGCCGTGGGATTGCAGCAGATCCACGTAGGAg  <  2:884724/140‑1 (MQ=255)

AGAAGTCGTTACCTTTGTCATCGACCAGGATAAACGCCGGGAAATCTTCTACTTCGATTTTCCAGATAGCTTCCATACCCAGCTCCGGATAAGCGACGCACTCCAGATGCTTGATGCTCTGCTGCGCCAGTACCGCCGCCGGACCGCCGATGCTACCGAGGTAGAAGCCGCCGTGTTTATGACACGCGTCGGTAACCTGCTGACTGCGGTTACCTTTCGCCAGCATGATCATGCTGCCGCCGTGGGATTGCAGCAGATCCACGTAGGAG  >  NC_000913/4345724‑4345992

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 19 ≤` `ATCG/ATCG` `< 26 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`