Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,394,055	G→T	L5I (CTC→ATC)	queG ←	epoxyqueuosine reductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,394,055	0	G	T	97.2%	248.2 / 0.6	72	L5I (CTC→ATC)	queG	epoxyqueuosine reductase
Reads supporting (aligned to +/- strand):  ref base G (0/2);  new base T (33/37);  total (33/39)
Fisher's exact test for biased strand distribution p-value = 4.96e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.38e-01

CCGTGGTATTGTTTGTCCAGCCATGCTTGCAGTTTGGGCTCGGACTCGCTGAGATCGGTATCGGTAATACCTACCTGCTGAAAGCCCAGTTCCAGCCCCCACTGTTTAATTTTTTGCGCTAACTGATTGAGATCGAGGGGCTCTGACATGACGGACCATACAATGAAGAAAAACCCCGTAAGTATACCACACACCGTCTGGTACGCCGACGATATCCGCCGCGGAGAACGCGAGGCGGCAGATGTGCTGGGGCTCACACTCTATGAGCTGA  >  NC_000913/4393919‑4394189                                                                                                                                         |                                                                                                                                       ccGTGGTATTGTTTGTCCAGCCATGCTTGCAGTTTGGGCTCGGACTCGCTGAGATCGGTATCGGTAATACCTACCTGCTGAAAGCCCAGTTCCAGCCCCCACTGTTTAATTTTTTGCGCTAACTGATTGAGATCGAtggg                                                                                                                                     <  2:23818/140‑1 (MQ=255) ccGTGGTATTGTTTGTCCAGCCATGCTTGCAGTTTGGGCTCGGACTCGCTGAGATCGGTATCGGTAATACCTACCTGCTGAAAGCCCAGTTCCAGCCCCCACTGTTTAATTTTTTGCGCTAACTGATTGAGATCGAtggg                                                                                                                                     <  1:96561/140‑1 (MQ=255) ccGTGGTATTGTTTGTCCAGCCATGCTTGCAGTTTGGGCTCGGACTCGCTGAGATCGGTATCGGTAATACCTACCTGCTGAAAGCCCAGTTCCAGCCCCCACTGTTTAATTTTTTGCGCTAACTGATTGAGATCGAtggg                                                                                                                                     <  1:101125/140‑1 (MQ=255)    tGGTATTGTTTGTCCAGCCATGCTTGCAGTTTGGGCTCGGACTCGCTGAGATCGGTATCGGTAATACCTACCTGCTGAAAGCCCAGTTCCAGCCCCCACTGTTTAATTTTTTGCGCTAACTGATTGAGATCGATGGGctc                                                                                                                                  <  2:123064/140‑1 (MQ=255)       tATTGTTTGTCCAGCCATGCTTGCAGTTTGGGCTCGGACTCGCTGAGATCGGTATCGGTAATACCTACCTGCTGAAAGCCCAGTTCCAGCCCCCACTGTTTAATTTTTTGCGCTAACTGATTGAGATCGATGGGCTCTGa                                                                                                                               <  2:733060/140‑1 (MQ=255)       tATTGTTTGTCCAGCCATGCTTGCAGTTTGGGCTCGGACTCGCTGAGATCGGTATCGGTAATACCTACCTGCTGAAAGCCCAGTTCCAGCCCCCACTGTTTAATTTTTTGCGCTAACTGATTGAGATCGATGGGCTCTGa                                                                                                                               <  2:251507/140‑1 (MQ=255)           gtttgtCCAGCCATGCTTGCAGTTTGGGCTCGGACTCGCTGAGATCGGTATCGGTAATACCTACCTGCTGAAAGCCCAGTTCCAGCCCCCACTGTTTAATTTTTTGCGCTAACTGATTGAGATCGATGGGCTCTGACATg                                                                                                                           <  1:210127/140‑1 (MQ=255)           gtttgtCCAGCCATGCTTGCAGTTTGGGCTCGGACTCGCTGAGATCGGTATCGGTAATACCTACCTGCTGAAAGCCCAGTTCCAGCCCCCACTGTTTAATTTTTTGCGCTAACTGATTGAGATCGATGGGCTCTGACATg                                                                                                                           <  1:153411/140‑1 (MQ=255)             ttgtCCAGCCATGCTTGCAGTTTGGGCTCGGACTCGCTGAGATCGGTATCGGTAATACCTACCTGCTGAAAGCCCAGTTCCAGCCCCCACTGTTTAATTTTTTGCGCTAACTGATTGAGATCGATGGGCTCTGACATGAc                                                                                                                         <  1:1650678/140‑1 (MQ=255)             ttgtCCAGCCATGCTTGCAGTTTGGGCTCGGACTCGCTGAGATCGGTATCGGTAATACCTACCTGCTGAAAGCCCAGTTCCAGCCCCCACTGTTTAATTTTTTGCGCTAACTGATTGAGATCGATGGGCTCTGACATGAc                                                                                                                         <  2:408772/140‑1 (MQ=255)             ttgtCCAGCCATGCTTGCAGTTTGGGCTCGGACTCGCAGAGATCGGTATCGGTAATACCTACCTGCTGAAAGCCCAGTTCCAGCCCCCACTGTTTAATTTTTTGCGCTAACTGATTGAGATCGATGGGCTCTGACATGAc                                                                                                                         <  2:1314069/140‑1 (MQ=255)              tgtCCAGCCATGCTTGCAGTTTGGGCTCGGACTCGCTGAGATCGGTATCGGTAATACCTACCTGCTGAAAGCCCAGTTCCAGCCCCCACTGTTTAATTTTTTGCGCTAACTGATTGAGATCGATGGGCTCTGACATGACg                                                                                                                        >  2:128001/1‑140 (MQ=255)              tgtCCAGCCATGCTTGCAGTTTGGGCTCGGACTCGCTGAGATCGGTATCGGTAATACCTACCTGCTGAAAGCCCAGTTCCAGCCCCCACTGTTTAATTTTTTGCGCTAACTGATTGAGATCGATGGGCTCTGACATGACg                                                                                                                        <  1:1077064/140‑1 (MQ=255)              tgtCCAGCCATGCTTGCAGTTTGGGCTCGGACTCGCTGAGATCGGTATCGGTAATACCTACCTGCTGAAAGCCCAGTTCCAGCCCCCACTGTTTAATTTTTTGCGCTAACTGATTGAGATCGATGGGCTCTGACATGACg                                                                                                                        <  2:1915640/140‑1 (MQ=255)                tCCAGCCATGCTTGCAGTTTGGGCTCGGACTCGCTGAGATCGGTATCGGTAATACCTACCTGCTGAAAGCCCAGTTCCAGCCCCCACTGTTTAATTTTTTGCGCTAACTGATTGAGATCGATGGGCTCTGACATGACGGa                                                                                                                      >  1:915828/1‑140 (MQ=255)                tCCAGCCATGCTTGCAGTTTGGGCTCGGACTCGCTGAGATCGGTATCGGTAATACCTACCTGCTGAAAGCCCAGTTCCAGCCCCCACTGTTTAATTTTTTGCGCTAACTGATTGAGATCGATGGGCTCTGACATGACGGa                                                                                                                      <  1:688928/140‑1 (MQ=255)                tCCAGCCATGCTTGCAGTTTGGGCTCGGACTCGCTGAGATCGGTATCGGTAATACCTACCTGCTGAAAGCCCAGTTCCAGCCCCCACTGTTTAATTTTTTGCGCTAACTGATTGAGATCGATGGGCTCTGACATGACGGa                                                                                                                      >  2:305533/1‑140 (MQ=255)                tCCAGCCATGCTTGCAGTTTGGGCTCGGACTCGCTGAGATCGGTATCGGTAATACCTACCTGCTGAAAGCCCAGTTCCAGCCCCCACTGTTTAATTTTTTGCGCTAACTGATTGAGATCGATGGGCTCTGACATGACGGa                                                                                                                      >  1:1842039/1‑140 (MQ=255)                tCCAGCCATGCTTGCAGTTTGGGCTCGGACTCGCTGAGATCGGTATCGGTAATACCTACCTGCTGAAAGCCCAGTTCCAGCCCCCACTGTTTAATTTTTTGCGCTAACTGATTGAGATCGATGGGCTCTGACATGACGGa                                                                                                                      <  2:1842039/140‑1 (MQ=255)                     ccATGCTTGCAGTTTGGGCTCGGACTCGCTGAGATCGGTATCGGTAATACCTACCTGCTGAAAGCCCAGTTCCAGCCCCCACTGTTTAATTTTTTGCGCTAACTGATTGAGATCGAGGGGCTCTGACATGACGGACCATa                                                                                                                 <  1:205663/140‑1 (MQ=255)                      cATGCTTGCAGTTTGGGCTCGGACTCGCTGAGATCGGTATCGGTAATACCTACCTGCTGAAAGCCCAGTTCCAGCCCCCACTGTTTAATTTTTTGCGCTAACTGATTGAGATCGATGGGCTCTGACATGACGGACCATAc                                                                                                                >  2:1213950/1‑140 (MQ=255)                      cATGCTTGCAGTTTGGGCTCGGACTCGCTGAGATCGGTATCGGTAATACCTACCTGCTGAAAGCCCAGTTCCAGCCCCCACTGTTTAATTTTTTGCGCTAACTGATTGAGATCGATGGGCTCTGACATGACGGACCATAc                                                                                                                >  2:904206/1‑140 (MQ=255)                        tGCTTGCAGTTTGGGCTCGGACTCGCTGAGATCGGTATCGGTAATACCTACCTGCTGAAAGCCCAGTTCCAGCCCCCACTGTTTAATTTTTTGCGCTAACTGATTGAGATCGATGGGCTCTGACATGACGGACCATACaa                                                                                                              <  2:1437775/140‑1 (MQ=255)                          cTTGCAGTTTGGGCTCGGACTCGCTGAGATCGGTATCGGTAATACCTACCTGCTGAAAGCCCAGTTCCAGCCCCCACTGTTTAATTTTTTGCGCTAACTGATTGAGATCGATGGGCTCTGACATGACGGACCATACAATg                                                                                                            >  2:2182803/1‑140 (MQ=255)                          cTTGCAGTTTGGGCTCGGACTCGCTGAGATCGGTATCGGTAATACCTACCTGCTGAAAGCCCAGTTCCAGCCCCCACTGTTTAATTTTTTGCGCTAACTGATTGAGATCGATGGGCTCTGACATGACGGACCATACAATg                                                                                                            <  2:1136882/140‑1 (MQ=255)                               aGTTTGGGCTCGGACTCGCTGAGATCGGTATCGGTAATACCTACCTGCTGAAAGCCCAGTTCCAACCCCCACTGTTTAATTTTTTGCGCTAACTGATTGAGATCGATGGGCTCTGACATGACGGACCATACAATgaagaa                                                                                                       >  2:1790160/1‑140 (MQ=255)                                gTTTGGGCTCGGACTCGCTGAGATCGGTATCGGTAATACCTACCTGCTGAAAGCCCAGTTCCAGCCCCCACTGTTTAATTTTTTGCGCTAACTGATTGAGATCGATGGGCTCTGACATGACGGACCATACAATGAAGaaa                                                                                                      <  1:662051/140‑1 (MQ=255)                                   tGGGCTCGGACTCGCTGAGATCGGTATCGGTAATACCTACCTGCTGAAAGCCCAGTTCCAGCCCCCACTGTTTAATTTTTTGCGCTAACTGATTGAGATCGATGGGCTCTGACATGACGGACCATACAATGAAGAAAAAc                                                                                                   >  2:1619002/1‑140 (MQ=255)                                     ggCTCGGACTCGCTGAGATCGGTATCGGTAATACCTACCTGCTGAAAGCCCAGTTCCAGCCCCCACTGTTTAATTTTTTGCGCTAACTGATTGAGATCGATGGGCTCTGACATGACGGACCATACAATGAAGAAAAAccc                                                                                                 <  1:939344/140‑1 (MQ=255)                                     ggCTCGGACTCGCTGAGATCGGTATCGGTAATACCTACCTGCTGAAAGCCCAGTTCCAGCCCCCACTGTTTAATTTTTTGCGCTAACTGATTGAGATCGATGGGCTCTGACATGACGGACCATACAATGAAGAAAAAccc                                                                                                 <  2:220431/140‑1 (MQ=255)                                     ggCTCGGACTCGCTGAGATCGGTATCGGTAATACCTACCTGCTGAAAGCCCAGTTCCAGCCCCCACTGTTTAATTTTTTGCGCTAACTGATTGAGATCGATGGGCTCTGACATGACGGACCATACAATGAAGAAAAAccc                                                                                                 <  2:3936/140‑1 (MQ=255)                                     ggCTCGGACTCGCTGAGATCGGTATCGGTAATACCTACCTGCTGAAAGCCCAGTTCCAGCCCCCACTGTTTAATTTTTTGCGCTAACTGATTGAGATCGATGGGCTCTGACATGACGGACCATACAATGAAGAAAAAccc                                                                                                 <  1:904206/140‑1 (MQ=255)                                     ggCTCGGACTCGCTGAGATCGGTATCGGTAATACCTACCTGCTGAAAGCCCAGTTCCAGCCCCCACTGTTTAATTTTTTGCGCTAACTGATTGAGATCGATGGGCTCTGACATGACGGACCATACAATGAAGAAAAAccc                                                                                                 <  1:181593/140‑1 (MQ=255)                                      gCTCGGACTCGCTGAGATCGGTATCGGTAATACCTACCTGCTGAAAGCCCAGTTCCAGCCCCCACTGTTTAATTTTTTGCGCTAACTGATTGAGATCGATGGGCTCTGACATGACGGACCATACAATGAAGAAAAAcccc                                                                                                <  1:1646920/140‑1 (MQ=255)                                      gCTCGGAATCGCTGAGATCGGTTTCGGTAATTCCTACCTGTTGAAAGCCAAGTTCCAAGTCTAACTGTTTAATTTTTTGCGCTAACTGATTGAGAACGGTGGGCTCTGACATGTCGGACCATACAATGAAGAAACAcccc                                                                                                <  2:1548387/140‑1 (MQ=255)                                        tCGGACTCGCTGAGATCGGTATCGGTAATACCTACCTGCTGAAAGCCCAGTTCCAGCCCCCACTGTTTAATTTTTTGCGCTAACTGATTGAGATCGATGGGCTCTGACATGACGGACCATACAATGAAGAAAAACCCCGt                                                                                              <  2:114384/140‑1 (MQ=255)                                        tCGGACTCGCTGAGATCGGTATCGGTAATACCTACCTGCTGAAAGCCCAGTTCCAGCCCCCACTGTTTAATTTTTTGCGCTAACTGATTGAGATCGATGGGCTCTGACATGACGGACCATACAATGAAGAAAAACCCCGt                                                                                              <  2:1506559/140‑1 (MQ=255)                                          ggACTCGCTGAGATCGGTATCGGTAATACCTACCTGCTGAAAGCCCAGTTCCAGCCCCCACTGTTTAATTTTTTGCGCTAACTGATTGAGATCGATGGGCTCTGACATGACGGACCATACAATGAAGAAAAAcccc                                                                                                <  1:1614237/136‑1 (MQ=255)                                          ggACTCGCTGAGATCGGTATCGGTAATACCTACCTGCTGAAAGCCCAGTTCCAGCCCCCACTGTTTAATTTTTTGCGCTAACTGATTGAGATCGATGGGCTCTGACATGACGGACCATACAATGAAGAAAAAcccc                                                                                                >  2:1614237/1‑136 (MQ=255)                                            aCTCGCTGAGATCGGTATCGGTAATACCTACCTGCTGAAAGCCCAGTTCCAGCCCCCACTGTTTAATTTTTTGCGCTAACTGATTGAGATCGATGGGCTCTGACATGACGGACCATACAATGAAGAAAAACCCCGTAAGt                                                                                          <  1:2167407/140‑1 (MQ=255)                                             cTCGCTGAGATCGGTATCGGTAATACCTACCTGCTGAAAGCCCAGTTCCAGCCCCCACTGTTTAATTTTTTGCGCTAACTGATTGAGATCGATGGGCTCTGACATGACGGACCATACAATGAAGAAAAACCCCGTAAGta                                                                                         <  2:1270016/140‑1 (MQ=255)                                                gCTGAGATCGGTATCGGTAATACCTACCTGCTGAAAGCCCAGTTCCAGCCCCCACTGTTTAATTTTTTGCGCTAACTGATTGAGATCGATGGGCTCTGACATGACGGACCATACAATGAAGAAAAACCCCGTAAGTATAc                                                                                      >  1:1606366/1‑140 (MQ=255)                                                     gaTCGGTATCGGTAATACCTACCTGCTGAAAGCCCAGTTCCAGCCCCCACTGTTTAATTTTTTGCGCTAACTGATTGAGATCGATGGGCTCTGACATGACGGACCATACAATGAAGAAAAACCCCGTAAGTATACcacac                                                                                 >  1:1961939/1‑140 (MQ=255)                                                      atcggtatcggtAATACCTACCTGCTGAAAGCCCAGTTCCAGCCCCCACTGTTTAATTTTTTGCGCTAACTGATTGAGATCGATGGGCTCTGACATGACGGACCATACAATGAAGAAAAACCCCGTAAGTATACcacaca                                                                                <  2:670721/140‑1 (MQ=255)                                                          gtatcggtaATACCTACCTGCTGAAAGCCCAGTTCCAGCCCCCACTGTTTAATTTTTTGCGCTAACTGATTGAGATCGATGGGCTCTGACATGACGGACCATACAATGAAGAAAAACCCCGTAAGTATACCACACACCGt                                                                            >  1:867037/1‑140 (MQ=255)                                                           tatcggtaATACCTACCTGCTGAAAGCCCAGTTCCAGCCCCCACTGTTTAATTTTTTGCGCTAACTGATTGAGATCGATGGGCTCTGACATGACGGACCATACAACGAAGAAAAACCCCGTAAGTATACCACACACCGTc                                                                           <  2:796497/140‑1 (MQ=255)                                                              cggtaATACCTACCTGCTGAAAGCCCAGTTCCAGCCCCCACTGTTTAATTTTTTGCGCTAACTGATTGAGATCGATGGGCTCTGACATGACGGACCATACAATGAAGAAAAACCCCGTAAGTATACCACACACCGTCTgg                                                                        >  2:1957202/1‑140 (MQ=255)                                                              cggtaATACCTACCTGCTGAAAGCCCAGTTCCAGCCCCCACTGTTTAATTTTTTGCGCTAACTGATTGAGATCGATGGGCTCTGACATGACGGACCATACAATGAAGAAAAACCCCGTAAGTATACCACACACCGTCTgg                                                                        >  1:15854/1‑140 (MQ=255)                                                               ggtaATACCTACCTGCTGAAAGCCCAGTTCCAGCCCCCACTGTTTAATTTTTTGCGCTAACTGATTGAGATCGATGGGCTCTGACATGACGGACCATACAATGAAGAAAAACCCCGTAAGTATACCACACACCGTCTggg                                                                       >  2:1035585/1‑139 (MQ=255)                                                                         acctGCTGAAAGCCCAGTTCCAGCCCCCACTGTTTAATTTTTTGCGCTAACTGATTGAGATCGATGGGCTCTGACATGACGGACCATACAATGAAGAAAAACCCCGTAAGTATACCACACACCGTCTGGTACGCcgacga                                                             >  2:1347999/1‑140 (MQ=255)                                                                         acctGCTGAAAGCCCAGTTCCAGCCCCCACTGTTTAATTTTTTGCGCTAACTGATTGAGATCGATGGGCTCTGACATGACGGACCATACAATGAAGAAAAACCCCGTAAGTATACCACACACCGTCTGGTACGCcgacga                                                             >  2:591339/1‑140 (MQ=255)                                                                         acctGCTGAAAGCCCAGTTCCAGCCCCCACTGTTTAATTTTTTGCGCTAACTGATTGAGATCGATGGGCTCTGACATGACGGACCATACAATGAAGAAAAACCCCGTAAGTATACCACACACCGTCTGGTACGCcgacga                                                             >  1:1069966/1‑140 (MQ=255)                                                                         acctGCTGAAAGCCCAGTTCCAGCCCCCACTGTTTAATTTTTTGCGCTAACTGATTGAGATCGATGGGCTCTGACATGACGGACCATACAATGAAGAAAAACCCCGTAAGTATACCACACACCGTCTGGTACGCcgacga                                                             >  2:1358091/1‑140 (MQ=255)                                                                         acctGCTGAAAGCCCAGTTCCAGCCCCCACTGTTTAATTTTTTGCGCTAACTGATTGAGATCGATGGGCTCTGACATGACGGACCATACAATGAAGAAAAACCCCGTAAGTATACCACACACCGTCTGGTACGCcgacga                                                             >  1:740228/1‑140 (MQ=255)                                                                           ctgctgAAAGCCCAGTTCCAGCCCCCACTGTTTAATTTTTTGCGCTAACTGATTGAGATCGATGGGCTCTGACATGACGGACCATACAATGAAGAAAAACCCCGTAAGTATACCACACACCGTCTGGTACGCCGACGata                                                           >  2:432996/1‑140 (MQ=255)                                                                           ctgctgAAAGCCCAGTTCCAGCCCCCACTGTTTAATTTTTTGCGCTAACTGATTGAGATCGATGGGCTCTGACATGACGGACCATACAATGAAGAAAAACCCCGTAAGTATACCACACACCGTCTGGTACGCCGACGata                                                           >  1:760244/1‑140 (MQ=255)                                                                            tgctgAAAGCCCAGTTCCAGCCCCCACTGTTTAATTTTTTGCGCTAACTGATTGAGATCGATGGGCTCTGACATGACGGACCATACAATGAAGAAAAACCCCGTAAGTATACCACACACCGTCTGGTACGCCGACGatat                                                          <  1:1056921/140‑1 (MQ=255)                                                                             gctgAAAGCCCAGTTCCAGCCCCCACTGTTTAATTTTTTGCGCTAACTGATTGAGATCGATGGGCTCTGACATGACGGACCATACAATGAAGAAAAACCCCGTAAGTATACCACACACCGTCTGGTACGCCGACGATATc                                                         >  1:1824459/1‑140 (MQ=255)                                                                                 aaaGCCCAGTTCCAGCCCCCACTGTTTAATTTTTTGCGCTAACTGATTGAGATCGATGGGCTCTGACATGACGGACCATACAATGAAGAAAAACCCCGTAAGTATACCACACACCGTCTGGTACGCCGACGATATccgcc                                                     >  1:427667/1‑140 (MQ=255)                                                                                 aaaGCCCAGTTCCAGCCCCCACTGTTTAATTTTTTGCGCTAACTGATTGAGATCGATGGGCTCTGACATGACGGACCATACAATGAAGAAAAACCCCGTAAGTATACCACACACCGTCTGGTACGCCGACGATATccgcc                                                     >  2:2057954/1‑140 (MQ=255)                                                                                 aaaGCCCAGTTCCAGCCCCCACTGTTTAATTTTTTGCGCTAACTGATTGAGATCGATGGGCTCTGACATGACGGACCATACAATGAAGAAAAACCCCGTAAGTATACCACACACCGTCTGGTACGCCGACGATATccgcc                                                     >  1:2000168/1‑140 (MQ=255)                                                                                 aaaGCCCAGTTCCAGCCCCCACTGTTTAATTTTTTGCGCTAACTGATTGAGATCGATGGGCTCTGACATGACGGACCATACAATGAAGAAAAACCCCGTAAGTATACCACAAACCGTCTGGTACGCCGACGATATccgcc                                                     >  2:1251458/1‑140 (MQ=255)                                                                                   aGCCCAGTTCCAGCCCCCACTGTTTAATTTTTTGCGCTAACTGATTGAGATCGATGGGCTCTGACATGACGGACCATACAATGAAGAAAAACCCCGTAAGTATACCACACACCGTCTGGTACGCCGACGATATCcgccgc                                                   >  1:327482/1‑140 (MQ=255)                                                                                                    cACTGTTTAATTTTTTGCGCTAACTGATTGAGATCGATGGGCTCTGACATGACGGACCATACAATGAAGAAAAACCCCGTAAGTATACCACACACCGTCTGGTACGCCGACGATATCCGCCGCGGAGAACGCGAggcggc                                  >  1:469324/1‑140 (MQ=255)                                                                                                      cTGTTTAATTTTTTGCGCTAACTGATTGAGATCGATGGGCTCTGACATGACGGACCATACAATGAAGAAAAACCCCGTAAGTATACCACACACCGTCTGGTACGCCGACGATATCCGCCGCGGAGAACGCGAGGCGGCAg                                <  2:760244/140‑1 (MQ=255)                                                                                                       tGTTTAATTTTTTGCGCTAACTGATTGAGATCGATGGGCTCTGACATGACGGACCATACAATGAAGAAAAACCCCGTAAGTATACCACACACCGTCTGGTACGCCGACGATATCCGCCGCGGAGAACGCGAGGCGGCAGa                               <  1:591339/140‑1 (MQ=255)                                                                                                         tttAATTTTTTGCGCTAACTGATTGAGATCGATGGGCTCTGACATGACGGACCATACAATGAAGAAAAACCCCGTAAGTATACCACACACCGTCTGGTACGCCGACGATATCCGCCGCGGAGAACGCGAGGCGGCAGAtg                             >  2:378561/1‑140 (MQ=255)                                                                                                               tttttGCGCTAACTGATTGAGATCGATGGGCTCTGACATGACGGACCATACAATGAAGAAAAACCCCGTAAGTATACCACACACCGTCTGGTACGCCGACGATATCCGCCGCGGAGAACGCGAGGCGGCAGATGTGCTgg                       <  2:740228/140‑1 (MQ=255)                                                                                                                   tGCGCTAACTGATTGAGATCGATGGGCTCTGACATGACGGACCATACAATGAAGAAAAACCCCGTAAGTATACCACACACCGTCTGGTACGCCGACGATATCCGCCGCGGAGAACGCGAGGCGGCAGATGTGCTGGGGCt                   <  1:983392/140‑1 (MQ=255)                                                                                                                     cgcTAACTGATTGAGATCGATGGGCTCTGACATGACGGACCATACAATGAAGAAAAACCCCGTAAGTATACCACACACCGTCTGGTACGCCGACGATATCCGCCGCGGAGAACGCGAGGCGGCAGATGTGCTGGGGCTca                 <  1:378561/140‑1 (MQ=255)                                                                                                                     cgcTAACTGATTGAGATCGATGGGCTCTGACATGACGGACCATACAATGAAGAAAAACCCCGTAAGTATACCACACACCGTCTGGTACGCCGACGATATCCGCCGCGGAGAACGCGAGGCGGCAGATGTGCTGGGGCTca                 <  1:1672147/140‑1 (MQ=255)                                                                                                                     cgcTAACTGATTGAGATCGATGGGCTCTGACATGACGGACCATACAATGAAGAAAAACCCCGTAAGTATACCACACACCGTCTGGTACGCCGACGATATCCGCCGCGGAGAACGCGAGGCGGCAGATGTGCTGGGGCTca                 <  2:1656529/140‑1 (MQ=255)                                                                                                                      gcTAACTGATTGAGATCGATGGGCTCTGACATGACGGACCATACAATGAAGAAAAACCCCGTAAGTATACCACACACCGTCTGGTACGCCGACGATATCCGCCGCGGAGAACGCGAGGCGGCAGATGTGCTGGGGCTcac                <  1:1347999/140‑1 (MQ=255)                                                                                                                        tAACTGATTGAGATCGAGGGGCTCTGACATGACGGACCATACAATGAAGAAAAACCCCGTAAGTATACCACACACCGTCTGGTACGCCGACGATATCCGCCGCGGAGAACGCGAGGCGGCAGATGTGCTGGGGCTCacac              <  1:703518/140‑1 (MQ=255)                                                                                                                            tGATTGAGATCGATGGGCTCTGACATGACGGACCATACAATGAAGAAAAACCCCGTAAGTATACCACACACCGTCTGGTACGCCGACGATATCCGCCGCGGAGAACGCGAGGCGGCAGATGTGCTGGGGCTCACACTCTa          <  1:1015724/140‑1 (MQ=255)                                                                                                                                  agaTCGATGGGCTCTGACATGACGGACCATACAATGAAGAAAAACCCCGTAAGTATACCACACACCGTCTGGTACGCCGACGATATCCGCCGCGGAGAACGCGAGGCGGCAGATGTGCTGGGGCTCACACTCTATGAGCt    >  1:1777427/1‑140 (MQ=255)                                                                                                                                    aTCGATGGGCTCTGACATGACGGACCATACAATGAAGAAAAACCCCGTAAGTATACCACACACCGTCTGGTACGCCGACGATATCCGCCGCGGAGAACGCGAGGCGGCAGATGTGCTGGGGCTCACACTCTATGAGCTGa  >  1:33751/1‑140 (MQ=255)                                                                                                                                    aTCGATGGGCTCTGACATGACGGACCATAAAATGAAGAAAAACCCCGTAAGTATACCACACACCGTCTGGTACGCCGACGATATCCGCCGCGGAGAACGCGAGGCGGCAGATGTGCTGGGGCTCACACGCTGTGAGCTGa  >  2:1635739/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                       CCGTGGTATTGTTTGTCCAGCCATGCTTGCAGTTTGGGCTCGGACTCGCTGAGATCGGTATCGGTAATACCTACCTGCTGAAAGCCCAGTTCCAGCCCCCACTGTTTAATTTTTTGCGCTAACTGATTGAGATCGAGGGGCTCTGACATGACGGACCATACAATGAAGAAAAACCCCGTAAGTATACCACACACCGTCTGGTACGCCGACGATATCCGCCGCGGAGAACGCGAGGCGGCAGATGTGCTGGGGCTCACACTCTATGAGCTGA  >  NC_000913/4393919‑4394189

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑