BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	781,471	C→G	intergenic (+27/‑106)	lysZ → / → lysQ	tRNA‑Lys/tRNA‑Lys

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	781,471	0	C	G	99.2%	457.5 / ‑3.8	123	intergenic (+27/‑106)	lysZ/lysQ	tRNA‑Lys/tRNA‑Lys
Reads supporting (aligned to +/- strand): ref base C (0/1); new base G (58/64); total (58/65)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.70e-01

Only 100 of 123 total aligned reads displayed.

GCCCCGAGTAAAAATCTTTCAGGTAACACCCGTATGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATTTAAAGGTGGTTACTGGTAGAGAACGTGAAGGATAACGTTGCGTTAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCCACCATCCTGAATGATTAAGGCAGCATAATCCCGCAAGGGGTCGTTAGCTCAGTTGGTAGAGCAGTTG  >  NC_000913/781334‑781606

gCCCCGAGTAAAAATCTTTCAGGTAACACCCGTATGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATTTAAAGGTGGTTACTGGTAGAGAAGGt                                                                                                                                       >  1:1772202/1‑140 (MQ=255)

gCCCCGAGTAAAAATCTTTCAGGTAACACCCGTATGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATTTAAAGGTGGTTACTGGTAGAGAAGGt                                                                                                                                       >  2:1342142/1‑140 (MQ=255)

 ccccGAGTAAAAATCTTTCAGGTAACACCCGTATGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATTTAAAGGTGGTTACTGGTAGAGAAGGTg                                                                                                                                      <  1:969624/140‑1 (MQ=255)

      aGTAAAAATCTTTCAGGTAACACCCGTATTGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATTTAAAGGTGGTTACTGGTAGAGAAGGTGAAGGa                                                                                                                                 >  1:787545/1‑140 (MQ=255)

           aaaTCTTTCAGGTAACACCCGTATGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACTCACCAATTTAAAGGTGGTTACTGGTAGAGAAGGTGAAGGATAACg                                                                                                                            <  1:2192171/140‑1 (MQ=255)

                 ttCAGGTAACACCCGTATGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATTTAAAGGTGGTTACTGGTAGAGAAGGTGAAGGATAACGTTGCGt                                                                                                                      <  2:1527577/140‑1 (MQ=255)

                  tCAGGTAACACCCGTATGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATTTAAAGGTGGTTACTGGTAGAGAAGGTGAAGGATAACGTTGCGtt                                                                                                                     >  1:1926506/1‑140 (MQ=255)

                  tCAGGTAACACCCGTATGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATTTAAAGGTGGTTACTGGTAGAGAAGGTGAAGGATAACGTTGCGtt                                                                                                                     <  1:1598498/140‑1 (MQ=255)

                       tAACACCCGTATGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATTTAAAGGTGGTTACTGGTAGAGAAGGTGAAGGATAACGTTGCGTTAGCaa                                                                                                                <  2:1263214/140‑1 (MQ=255)

                        aaCACCCGTATGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATTTAAAGGTGGTTACTGGTAGAGAAGGTGAAGGATAACGTTGCGTTAGCAAc                                                                                                               <  1:2100409/140‑1 (MQ=255)

                               gTATGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATTTAAAGGTGGTTACTGGTAGAGAAGGTGAAGGATAACGTTGCGTTAGCAACGGCCCGa                                                                                                        <  1:364438/140‑1 (MQ=255)

                                tATGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATTTAAAGGTGGTTACTGGTAGAGAAGGTGAAGGATAACGTTGCGTTAGCAACGGCCCGaa                                                                                                       >  2:1341204/1‑140 (MQ=255)

                                tATGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATTTAAAGGTGGTTACTGGTAGAGAAGGTGAAGGATAACGTTGCGTTAGCAACGGCCCGaa                                                                                                       >  2:1322035/1‑140 (MQ=255)

                                tATGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATTTAAAGGTGGTTACTGGTAGAGAAGGTGAAGGATAACGTTGCGTTAGCAACGGCCCGaa                                                                                                       >  1:1157731/1‑140 (MQ=255)

                                     gTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATTTAAAGGTGGTTACTGGTAGAGAAGGTGAAGGATAACGTTGCGTTAGCAACGGCCCGAAGGGCg                                                                                                  >  2:2035345/1‑140 (MQ=255)

                                     gTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATTTAAAGGTGGTTACTGGTAGAGAAGGTGAAGGATAACGTTGCGTTAGCAACGGCCCGAAGGGCg                                                                                                  >  2:884738/1‑140 (MQ=255)

                                       cGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATTTAAAGGTGGTTACTGGTAGAGAAGGTGAAGGATAACGTTGCGTTAGCAACGGCCCGAAGGGCgag                                                                                                >  1:574421/1‑140 (MQ=255)

                                        gTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATTTAAAGGTGGTTACTGGTAGAGAAGGTGAAGGATAACGTTGCGTTAGCAACGGCCCGAAggg                                                                                                    <  2:1227835/135‑1 (MQ=255)

                                        gTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATTTAAAGGTGGTTACTGGTAGAGAAGGTGAAGGATAACGTTGCGTTAGCAACGGCCCGAAggg                                                                                                    >  1:1227835/1‑135 (MQ=255)

                                        gTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATTTAAAGGTGGTTACTGGTAGAGAAGGTGAAGGATAACGTTGCGTTAGCAACGGCCCGAAGGGCgaga                                                                                               >  1:1369124/1‑140 (MQ=255)

                                           aGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATTTAAAGGTGGTTACTGGTAGAGAAGGTGAAGGATAACGTTGCGTTAGCAACGGCCCGAAGGGCGAGACGa                                                                                            <  1:1753929/140‑1 (MQ=255)

                                           aGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATTTAAAGGTGGTTACTGGTAGAGAAGGTGAAGGATAACGTTGCGTTAGCAACGGCCCGAAGGGCGAGACGa                                                                                            <  2:2074143/140‑1 (MQ=255)

                                             cTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATTTAAAGGTGGTTACTGGTAGAGAAGGTGAAGGATAACGTTGCGTTAGCAACGGCCCGAAGGGCGAGACGAAg                                                                                          <  2:399742/140‑1 (MQ=255)

                                             cTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATTTAAAGGTGGTTACTGGTAGAGAAGGTGAAGGATAACGTTGCGTTAGCAACGGCCCGAAGGGCGAGACGAAg                                                                                          <  1:1915800/140‑1 (MQ=255)

                                               cAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATTTAAAGGTGGTTACTGGTAGAGAAGGTGAAGGATAACGTTGCGTTAGCAACGGCCCGAAGGGCGAGACGAAGTc                                                                                        >  2:1758900/1‑140 (MQ=255)

                                                  ttGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATTTAAAGGTGGTTACTGGTAGAGAAGGTGAAGGATAACGTTGCGTTAGCAACGGCCCGAAGGGCGAGACGAAg                                                                                          >  1:945342/1‑135 (MQ=255)

                                                  ttGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATTTAAAGGTGGTTACTGGTAGAGAAGGTGAAGGATAACGTTGCGTTAGCAACGGCCCGAAGGGCGAGACGAAg                                                                                          <  2:945342/135‑1 (MQ=255)

                                                  ttGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATTTAAAGGTGGTTACTGGTAGAGAAGGTGAAGGATAACGTTGCGTTAGCAACGGCCCGAAGGGCGAGACGAAGTCGAg                                                                                     >  2:2016542/1‑140 (MQ=255)

                                                  ttGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATTTAAAGGTGGTTACTGGTAGAGAAGGTGAAGGATAACGTTGCGTTAGCAACGGCCCGAAGGGCGAGACGAAGTCGAg                                                                                     <  2:574421/140‑1 (MQ=255)

                                                  ttGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATTTAAAGGTGGTTACTGGTAGAGAAGGTGAAGGATAACGTTGCGTTAGCAACGGCCCGAAGGGCGAGACGAAGTCGAg                                                                                     <  1:1341204/140‑1 (MQ=255)

                                                          gCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCACTTTAAAGGTGGTTACTGGTAGAGAAGGTGAAGGATAACGTTGCGTTAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTg                                                                             >  1:1797916/1‑140 (MQ=255)

                                                          gCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATTTAAAGGTGGTTACTGGTAGAGAAGGTGAAGGATAACGTTGCGTTAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTg                                                                             >  2:929767/1‑140 (MQ=255)

                                                          gCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATTTAAAGGTGGTTACTGGTAGAGAAGGTGAAGGATAACGTTGCGTTAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTg                                                                             >  1:1985483/1‑140 (MQ=255)

                                                          gCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATTTAAAGGTGGTTACTGGTAGAGAAGGTGAAGGATAACGTTGCGTTAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTg                                                                             <  2:362073/140‑1 (MQ=255)

                                                            aGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATTTAAAGGTGGTTACTGGTAGAGAAGGTGAAGGATAACGTTGCGTTAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCa                                                                           <  2:1297645/140‑1 (MQ=255)

                                                            aGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATTTAAAGGTGGTTACTGGTAGAGAAGGTGAAGGATAACGTTGCGTTAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCa                                                                           <  2:1722414/140‑1 (MQ=255)

                                                            aGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATTTAAAGGTGGTTACTGGTAGAGAAGGTGAAGGATAACGTTGCGTTAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCa                                                                           >  2:172298/1‑140 (MQ=255)

                                                            aGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATTTAAAGGTGGTTACTGGTAGAGAAGGTGAAGGATAACGTTGCGTTAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCa                                                                           <  2:2033434/140‑1 (MQ=255)

                                                             gTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATTTAAAGGTGGTTACTGGTAGAGAAGGTGAAGGATAACGTTGCGTTAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCAc                                                                          >  2:1856232/1‑140 (MQ=255)

                                                              ttGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATTTAAAGGTGGTTACTGGTAGAGAAGGTGAAGGATAACGTTGCGTTAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACg                                                                         <  1:461090/140‑1 (MQ=255)

                                                              ttGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATTTAAAGGTGGTTACTGGTAGAGAAGGTGAAGGATAACGTTGCGTTAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACg                                                                         >  2:1845553/1‑140 (MQ=255)

                                                               tGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATTTAAAGGTGGTTACTGGTAGAGAAGGTGAAGGATAACGTTGCGTTAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGa                                                                        >  2:2169075/1‑140 (MQ=255)

                                                                   ttttAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATTTAAAGGTGGTTACTGGTAGAGAAGGTGAAGGATAACGTTGCGTTAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACcca                                                                    <  2:1649354/140‑1 (MQ=255)

                                                                   ttttAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATTTAAAGGTGGTTACTGGTAGAGAAGGTGAAGGATAACGTTGCGTTAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACcca                                                                    <  1:2096001/140‑1 (MQ=255)

                                                                   ttttAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATTTAAAGGTGGTTACTGGTAGAGAAGGTGAAGGATAACGTTGCGTTAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACcca                                                                    <  2:1119518/140‑1 (MQ=255)

                                                                   ttttAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATTTAAAGGTGGTTACTGGTAGAGAAGGTGAAGGATAACGTTGCGTTAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACcca                                                                    <  1:1342142/140‑1 (MQ=255)

                                                                        aTCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATTTAAAGGTGGTTACTGGTAGAGAAGGTGAAGGATAACGTTGCGTTAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCCACCATc                                                               >  1:2218120/1‑140 (MQ=255)

                                                                        aTCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATTTAAAGGAGGTTACTGGTAGAGAAGGTGAAGGATAACGTTGCGTTAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCCACCATc                                                               >  2:1515068/1‑140 (MQ=255)

                                                                           aaTTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATTTAAAGGTGGTTACTGGTAGAGAAGGTGAAGGATAACGTTGCGTTAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCCACCATCCTg                                                            <  1:929767/140‑1 (MQ=255)

                                                                            aTTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATTTAAAGGTGGTTACTGGTAGAGAAGGTGAAGGATAACGTTGCGTTAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCCACCATCCTGa                                                           <  1:1722901/140‑1 (MQ=255)

                                                                             ttGGTCGCAGGTTCGAATCCTGCACGACCCACCAATTTAAAGGTGGTTACTGGTAGAGAAGGTGAAGGATAACGTTGCGTTAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCCACCATCCTGaa                                                          >  2:225297/1‑140 (MQ=255)

                                                                             ttGGTCGCAGGTTCGAATCCTGCACGACCCACCAATTTAAAGGTGGTTACTGGTAGAGAAGGTGAAGGATAACGTTGCGTTAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCCACCATCCTGaa                                                          >  2:2041022/1‑140 (MQ=255)

                                                                               ggTCGCAGGTTCGAATCCTGCACGACCCACCAATTTAAAGGTGGTTACTGGTAGAGAAGGTGAAGGATAACGTTGCGTTAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCCACCATCCTGAATg                                                        >  2:1715578/1‑140 (MQ=255)

                                                                                gTCGCAGGTTCGAATCCTGCACGACCCACCAATTTAAAGGTGGTTACTGGTAGAGAAGGTGAAGGATAACGTTGCGTTAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCCACCATCCTGAATGa                                                       >  1:1553859/1‑140 (MQ=255)

                                                                                 tCGCAGGTTCGAATCCTGCACGACCCACCAATTTAAAGGTGGTTACTGGTAGAGAAGGTGAAGGATAACGTTGCGTTAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCCACCATCCTGAATGAt                                                      <  2:1709682/140‑1 (MQ=255)

                                                                                 tCGCAGGTTCGAATCCTGCACGACCCACCAATTTAAAGGTGGTTACTGGTAGAGAAGGTGAAGGATAACGTTGCGTTAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCCACCATCCTGAATGAt                                                      <  1:186197/140‑1 (MQ=255)

                                                                                      ggTTCGAATCCTGCACGACCCACCAATTTAAAGGTGGTTACTGGTAGAGAAGGTGAAGGATAACGTTGCGTTAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCCACCATCCTGAATGATTAAgg                                                 <  1:2016542/140‑1 (MQ=255)

                                                                                       gTTCGAATCCTGCACGACCCACCAATTTAAAGGTGGTTACTGGTAGAGAAGGTGAAGGATAACGTTGCGTTAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCCACCATCCTGAATGATTAAGgc                                                >  2:1943387/1‑140 (MQ=255)

                                                                                         tCGAATCCTGCACGACCCACCAATTTAAAGGTGGTTACTGGTAGAGAAGGTGAAGGATAACGTTGCGTTAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCCACCATCCTGAATGATTAAGgcag                                              <  1:1611964/140‑1 (MQ=255)

                                                                                         tCGAATCCTGCACGACCCACCAATTTAAAGGTGGTTACTGGTAGAGAAGGTGAAGGATAACGTTGCGTTAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCCACCATCCTGAATGATTAAGgcag                                              <  1:884738/140‑1 (MQ=255)

                                                                                             aTCCTGCACGACCCACCAATTTAAAGGTGGTTACTGGTAGAGAAGGTGAAGGATAACGTTGCGTTAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCCACCATCCTGAATGATTAAGGCAGCATa                                          >  2:439240/1‑140 (MQ=255)

                                                                                              tCCTGCACGACCCACCAATTTAAAGGTGGTTACTGGTAGAGAAGGTGAAGGATAACGTTGCGTTAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCCACCATCCTGAATGATTAAGGCAGCATaa                                         >  1:669271/1‑140 (MQ=255)

                                                                                                 tGCACGACCCACCAATTTAAAGGTGGTTACTGGTAGAGAAGGTGAAGGATAACGTTGCGTTAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCCACCATCCTGAATGATTAAGGCAGCATAATcc                                      >  1:1150819/1‑140 (MQ=255)

                                                                                                 tGCACGACCCACCAATTTAAAGGTGGTTACTGGTAGAGAAGGTGAAGGATAACGTTGCGTTAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCCACCATCCTGAATGATTAAGGCAGCATAATcc                                      <  2:385770/140‑1 (MQ=255)

                                                                                                   cACGACCCACCAATTTAAAGGTGGTTACTGGTAGAGAAGGTGAAGGATAACGTTGCGTTAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCCACCATCCTGAATGAt                                                      <  2:1909476/122‑1 (MQ=255)

                                                                                                   cACGACCCACCAATTTAAAGGTGGTTACTGGTAGAGAAGGTGAAGGATAACGTTGCGTTAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCCACCATCCTGAATGAt                                                      >  1:1909476/1‑122 (MQ=255)

                                                                                                   cACGACCCACCAATTTAAAGGTGGTTACTGGTAGAGAAGGTGAAGGATAACGTTGCGTTAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCCACCATCCTGAATGATTAAGGCAGCATAATCCCg                                    >  2:447993/1‑140 (MQ=255)

                                                                                                    aCGACCCACCAATTTAAAGGTGGTTACTGGTAGAGAAGGTGAAGGATAACGTTGCGTTAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCCACCATCCTGAATGATTAAGGCAGCATAATCCCGc                                   <  2:1008879/140‑1 (MQ=255)

                                                                                                    aCGACCCACCAATTTAAAGGTGGTTACTGGTAGAGAAGGTGAAGGATAACGTTGCGTTAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCCACCATCCTGAATGATTAAGGCAGCATAATCCCGc                                   <  2:1553859/140‑1 (MQ=255)

                                                                                                        cccACCAATTTAAAGGTGGTTACTGGTAGAGAAGGTGAAGGATAACGTTGCGTTAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCCACCATCCTGAATGATTAAGGCAGCATAATCCCGCAAgg                               >  2:1493499/1‑140 (MQ=255)

                                                                                                        cccACCAATTTAAAGGTGGTTACTGGTAGAGAAGGTGAAGGATAACGTTGCGTTAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCCACCATCCTGAATGATTAAGGCAGCATAATCCCGCAAgg                               <  1:1322035/140‑1 (MQ=255)

                                                                                                         ccaccaATTTAAAGGTGGTTACTGGTAGAGAAGGTGAAGGATAACGTTGCGTTAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCCACCATCCTGAATGATTAAGGCAGCATAATCCCTCAAggg                              <  1:518415/140‑1 (MQ=255)

                                                                                                           accaATTTAAAGGTGGTTACTGGTAGAGAAGGTGAAGGATAACGTTGCGTTAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCCACCATCCTGAATGATTAAGGCAGCATAATcc                                      <  1:246313/130‑1 (MQ=255)

                                                                                                           accaATTTAAAGGTGGTTACTGGTAGAGAAGGTGAAGGATAACGTTGCGTTAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCCACCATCCTGAATGATTAAGGCAGCATAATcc                                      >  2:246313/1‑130 (MQ=255)

                                                                                                           accaATTTAAAGGTGGTTACTGGTAGAGAAGGTGAAGGATAACGTTGCGTTAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCCACCATCCTGAATGATTAAGGCAGCATAATCCCGCAAGGGGt                            <  2:1157731/140‑1 (MQ=255)

                                                                                                           accaATTTAAAGGTGGTTACTGGTAGAGAAGGTGAAGGATAACGTTGCGTTAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCCACCATCCTGAATGATTAAGGCAGCATAATCCCGCAAGGGGt                            >  2:1965603/1‑140 (MQ=255)

                                                                                                               aTTTAAAGGTGGTTACTGGTAGAGAAGGTGAAGGATAACGTTGCGTTAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCCACCATCCTGAATGATTAAGGCAGCATAATCCCGCAAGGGGTCGtt                        <  1:161042/140‑1 (MQ=255)

                                                                                                                  tAAAGGTGGTTACTGGTAGAGAAGGTGAAGGATAACGTTGCGTTAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCCACCATCCTGAATGATTAAGGCAGCATAATCCCGCAAGGGGTCGTTAGc                     <  1:1602388/140‑1 (MQ=255)

                                                                                                                  tAAAGGTGGTTACTGGTAGAGAAGGTGAAGGATAACGTTGCGTTAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCCACCATCCTGAATGATTAAGGCAGCATAATCCCGCAAGGGGTCGTTAGc                     >  2:1866386/1‑140 (MQ=255)

                                                                                                                  tAAAGGTGGTTACTGGTAGAGAAGGTGAAGGATAACGTTGCGTTAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCCACCATCCTGAATGATTAAGGCAGCATAATCCCGCAAGGGGTCGTTAGc                     >  2:1768339/1‑140 (MQ=255)

                                                                                                                  tAAAGGTGGTTACTGGTAGAGAAGGTGAAGGATAACGTTGCGTTAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCCACCATCCTGAATGATTAAGGCAGCATAATCCCGCAAGGGGTCGTTAGc                     >  1:2150354/1‑140 (MQ=255)

                                                                                                                  tAAAGGTGGTTACTGGTAGAGAAGGTGAAGGATAACGTTGCGTTAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCCACCATCCTGAATGATTAAGGCAGCATAATCCCGCAAGGGGTCGTTAGc                     >  1:581047/1‑140 (MQ=255)

                                                                                                                   aaaGGTGGTTACTGGTAGAGAAGGTGAAGGATAACGTTGCGTTAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCCACCATCCTGAATGATTAAGGCAGCATAATCCCGCAAGGGGTCGTTAGCt                    <  1:1866386/140‑1 (MQ=255)

                                                                                                                   aaaGGTGGTTACTGGTAGAGAAGGTGAAGGATAACGTTGCGTTAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCCACCATCCTGAATGATTAAGGCAGCATAATCCCGCAAGGGGTCGTTAGCt                    <  2:411622/140‑1 (MQ=255)

                                                                                                                    aaGGTGGTTACTGGTAGAGAAGGTGAAGGATAACGTTGCGTTAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCCACCATCCTGAATGATTAAGGCAGCATAATCCCGCAAGGGGTCGTTAGCTc                   <  1:1756442/140‑1 (MQ=255)

                                                                                                                    aaGGTGGTTACTGGTAGAGAAGGTGAAGGATAACGTTGCGTTAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCCACCATCCTGAATGATTAAGGCAGCATAATCCCGCAAGGGGTCGTTAGCTc                   <  1:659943/140‑1 (MQ=255)

                                                                                                                    aaGGTGGTTACTGGTAGAGAAGGTGAAGGATAACGTTGCGTTAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCCACCATCCTGAATGATTAAGGCAGCATAATCCCGCAAGGGGTCGTTAGCTc                   <  1:358555/140‑1 (MQ=255)

                                                                                                                     aGGTGGTTACTGGTAGAGAAGGTGAAGGATAACGTTGCGTTAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCCACCATCCTGAATGATTAAGGCAGCATAATCCCGCAAGGGGTCGTTAGCTCa                  <  1:1758900/140‑1 (MQ=255)

                                                                                                                          gtTACTGGTAGAGAAGGTGAAGGATAACGTTGCGTTAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCCACCATCCTGAATGATTAAGGCAGCATAATCCCGCAAGGGGTCGTTAGCTCAGTTgg             >  1:1066777/1‑140 (MQ=255)

                                                                                                                          gtTACTGGTAGAGAAGGTGAAGGATAACGTTGCGTTAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCCACCATCCTGAATGATTAAGGCAGCATAATCCCGCAAGGGGTCGTTAGCTCAGTTgg             >  2:1929944/1‑140 (MQ=255)

                                                                                                                               tGGTAGAGAACGTGAAGGATAACGTTGCGTTAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCCACCATCCTGAATGATTAAGGCAGCATAATCCCGCAAGGGGTCGTTAGCTCAGTTGGTagag        <  1:2152176/140‑1 (MQ=255)

                                                                                                                                  tAGAGAAGGTGAAGGATAACGTTGCGTTAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCCACCATCCTGAATGATTAAGGCAGCATAATCCCGCAAGGGGTCGTTAGCTCAGTTGGTAGAGCAg     <  1:2196679/140‑1 (MQ=255)

                                                                                                                                    gagaAGGTGAAGGATAACGTTGCGTTAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCCACCATCCTGAATGATTAAGGCAGCATAATCCCGCAAGGGGTCGTTAGCTCAGTTGGTAGAGCAGtt   >  1:534706/1‑140 (MQ=255)

                                                                                                                                    gagaAGGTGAAGGATAACGTTGCGTTAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCCACCATCCTGAATGATTAAGGCAGCATAATCCCGCAAGGGGTCGTTAGCTCAGTTGGTAGAGCAGtt   >  1:369291/1‑140 (MQ=255)

                                                                                                                                    gagaAGGTGAAGGATAACGTTGCGTTAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCCACCATCCTGAATGATTAAGGCAGCATAATCCCGCAAGGGGTCGTTAGCTCAGTTGGTAGAGCAGtt   >  2:365585/1‑140 (MQ=255)

                                                                                                                                    gagaAGGTGAAGGATAACGTTGCGTTAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCCACCATCCTGAATGATTAAGGCAGCATAATCCCGCAAGGGGTCGTTAGCTCAGTTGGTAGAGCAGtt   <  2:817771/140‑1 (MQ=255)

                                                                                                                                    gagaAGGTGAAGGATAACGTTGCGTTAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCCACCATCCTGAATGATTAAGGCAGCATAATCCCGCAAGGGGTCGTTAGCTCAGTTGGTAGAGCAGtt   >  1:80807/1‑140 (MQ=255)

                                                                                                                                    gagaAGGTGAAGGATAACGTTGCGTTAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCCACCATCCTGAATGATTAAGGCAGCATAATCCCGCAAGGGGTCGTTAGCTCAGTTGGTAGAGCAGtt   >  1:1035509/1‑140 (MQ=255)

                                                                                                                                     agaAGGTGAAGGATAACGTTGCGTTAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCCACCATCCTGAATGATTAAGGCAGCATAATCCCGCAAGGGGTCGTTAGCTCAGTTGGTAGAGCAGTTg  >  1:441147/1‑140 (MQ=255)

                                                                                                                                     agaAGGTGAAGGATAACGTTGCGTTAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCCACCATCCTGAATGATTAAGGCAGCATAATCCCGCAAGGGATCGTTAGCTCAGTTGGTAGAGCAGTTg  >  2:486647/1‑140 (MQ=255)

GCCCCGAGTAAAAATCTTTCAGGTAACACCCGTATGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATTTAAAGGTGGTTACTGGTAGAGAACGTGAAGGATAACGTTGCGTTAGCAACGGCCCGAAGGGCGAGACGAAGTCGAGTCATCCTGCACGACCCACCATCCTGAATGATTAAGGCAGCATAATCCCGCAAGGGGTCGTTAGCTCAGTTGGTAGAGCAGTTG  >  NC_000913/781334‑781606

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`