Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,678,604 G→T A60S (GCA→TCA)  ydgH → DUF1471 domain‑containing protein YdgH

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,678,6040GT98.6% 490.9 / ‑1.5 138coding (178/945 nt)ydgHDUF1471 domain‑containing protein YdgH
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base T (57/79);  minor base . (1/1);  total (58/80)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.62e-01

Only 100 of 139 total aligned reads displayed.

CTGCTATGGCATTCTCCGTTAACGCAGCAACAGAACTGACACCGGAGCAAGCGGCAGCGGTTAAACCTTTTGACCGTGTAGTGGTTACCGGTCGTTTTAATGCTATTGGCGAAGCGGTGAAAGCCGTTTCTCGTCGCGCAGATAAAGAAGGTGCCGCCTCTTTTTATGTTGTCGACACTTCTGATTTTGGTAACAGCGGTAACTGGCGTGTGGTCGCTGACCTCTATAAAGCCGATGCTGAAAAAGCAGAAGAAACAAGTAATCGCGTAATTAA  >  NC_000913/1678467‑1678740
                                                                                                                                         |                                                                                                                                        
cTGCTATGGCATTCTCCGTTAACGCAGCAACAGAACTGACACCGGAGCAAGCGGCAGCGGTTAAACCTTTTGACCGTGTAGTGGTTACCGGTCGTTTTAATGCTATTGGCGAAGCGGTGAAAGCCGTTTCTCGTCGCTCa                                                                                                                                        >  2:1274662/1‑140 (MQ=255)
 tGCTATGGCATTCTCCGTTAACGCAGCAACAGAACTGACACCGGAGCAAGCGGCAGCGGTTAAACCTTTTGACCGTGTAGTGGTTACCGGTCGTTTTAATGCTATTGGCGAAGCGGTGAAAGCCGTTTCTCGTCGCTCAg                                                                                                                                       <  2:1155937/140‑1 (MQ=255)
    tATGGCATTCTCCGTTAACGCAGCAACAGAACTGACACCGGAGCAAGCGGCAGCGGTTAAACCTTTTGACCGTGTAGTGGTTACCGGTCGTTTTAATGCTATTGGCGAAGCGGTGAAAGCCGTTTCTCGTCGCTCAGATa                                                                                                                                    >  1:559831/1‑140 (MQ=255)
      tGGCATTCTCCGTTAACGCAGCAACAGAACTGACACCGGAGCAAGCGGCAGCGGTTAAACCTTTTGACCGTGTAGTGGTTACCGGTCGTTTTAATGCTATTGGCGAAGCGGTGAAAGCCGTTTCTCGTCGCTCAGATaaa                                                                                                                                  >  2:249072/1‑140 (MQ=255)
      tGGCATTCTCCGTTAACGCAGCAACAGAACTGACACCGGAGCAAGCGGCAGCGGTTAAACCTTTTGACCGTGTAGTGGTTACCGGTCGTTTTAATGCTATTGGCGAAGCGGTGAAAGCCGTTTCTCGTCGCTCAGATaaa                                                                                                                                  >  1:76144/1‑140 (MQ=255)
       ggCATTCTCCGTTAACGCAGCAACAGAACTGACACCTGAGCAAGCGGCAGCGGTTAAACCTTTTGACCGTGTAGTGGTTACCGGTCGTTTTAATGCTATTGGCGAAGCGGTGAAAGCCGTTTCTCGTCGCTCAGATAaag                                                                                                                                 <  2:1063450/140‑1 (MQ=255)
        gCATTCTCCGTTAACGCAGCAACAGAACTGACACCGGAGCAAGCGGCAGCGGTTAAACCTTTTGACCGTGTAGTGGTTACCGGTCGTTTTAATGCTATTGGCGAAGCGGTGAAAGCCGTTTCTCGTCGCTCAGATAaaga                                                                                                                                >  1:1673148/1‑140 (MQ=255)
        gCATTCTCCGTTAACGCAGCAACAGAACTGACACCGGAGCAAGCGGCAGCGGTTAAACCTTTTGACCGTGTAGTGGTTACCGGTCGTTTTAATGCTATTGGCGAAGCGGTGAAAGCCGTTTCTCGTCGCTCAGATAaaga                                                                                                                                <  1:1962889/140‑1 (MQ=255)
        gCATTCTCCGTTAACGCAGCAACAGAACTGACACCGGAGCAAGCGGCAGCGGTTAAACCTTTTGACCGTGTAGTGGTTACCGGTCGTTTTAATGCTATTGGCGAAGCGGTGAAAGCCGTTTCTCGTCGCTCAGATAaaga                                                                                                                                >  2:666720/1‑140 (MQ=255)
        gCATTCTCCGTTAACGCAGCAACAGAACTGACACCGGAGCAAGCGGCAGCGGTTAAACCTTTTGACCGTGTAGTGGTTACCGGTCGTTTTAATGCTATTGGCGAAGCGGTGAAAGCCGTTTCTCGTCGCTCAGATAaaga                                                                                                                                >  2:94116/1‑140 (MQ=255)
        gCATTCTCCGTTAACGCAGCAACAGAACTGACACCGGAGCAAGCGGCAGCGGTTAAACCTTTTGACCGTGTAGTGGTTACCGGTCGTTTTAATGCTATTGGCGAAGCGGTGAAAGCCGTTTCTCGTCGC‑TAGATAaagaa                                                                                                                               >  1:1195504/1‑140 (MQ=255)
           ttCTCCGTTAACGCAGCAACAGAACTGACACCGGAGCAAGCGGCAGCGGTTAAACCTTTTGACCGTGTAGTGGTTACCGGTCGTTTTAATGCTATTGGCGAAGCGGTGAAAGCCGTTTCTCGTCGCTCAGATAAAGAAgg                                                                                                                             >  1:2010158/1‑140 (MQ=255)
           ttCTCCGTTAACGCAGCAACAGAACTGACACCGGAGCAAGCGGCAGCGGTTAAACCTTTTGACCGTGTAGTGGTTACCGGTCGTTTTAATGCTATTGGCGAAGCGGTGAAAGCCGTTTCTCGTCGCTCAGATAAAGAAgg                                                                                                                             >  2:943062/1‑140 (MQ=255)
             ctcCGTTAACGCAGCAACAGAACTGACACCGGAGCAAGCGGCAGCGGTTAAACCTTTTGACCGTGTAGTGGTTACCGGTCGTTTTAATGCTATTGGCGAAGCGGTGAAAGCCGTTTCTCGTCGCTCAGATAAAGAAGGTg                                                                                                                           >  2:718223/1‑140 (MQ=255)
             ctcCGTTAACGCAGCAACAGAACTGACACCGGAGCAAGCGGCAGCGGTTAAACCTTTTGACCGTGTAGTGGTTACCGGTCGTTTTAATGCTATTGGCGAAGCGGTGAAAGCCGTTTCTCGTCGCTCAGATAAAGAAGGTg                                                                                                                           >  1:151826/1‑140 (MQ=255)
               ccGTTAACGCAGCAACAGAACTGACACCGGAGCAAGCGGCAGCGGTTAAACCTTTTGACCGTGTAGTGGTTACCGGTCGTTTTAATGCTATTGGCGAAGCGGTGAAAGCCGTTTCTCGTCGCTCAGATAAAGAAGGTgcc                                                                                                                         <  2:1673148/140‑1 (MQ=255)
                 gTTAACGCAGCAACAGAACTGACACCGGAGCAAGCGGCAGCGGTTAAACCTTTTGACCGTGTAGTGGTTACCGGTCGTTTTAATGCTATTGGCGAAGCGGTGAAAGCCGTTTCTCGTCGCTCAGATAAAGAAGGTgccgc                                                                                                                       <  1:316835/140‑1 (MQ=255)
                 gTTAACGCAGCAACAGAACTGACACCGGAGCAAGCGGCAGCGGTTAAACCTTTTGACCGTGTAGTGGTTACCGGTCGTTTTAATGCTATTGGCGAAGCGGTGAAAGCCGTTTCTCGTCGCTCAGATAAAGAAGGTgccgc                                                                                                                       <  2:2055129/140‑1 (MQ=255)
                  ttAACGCAGCAACAGAACTGACACCGGAGCAAGCGGCAGCGGTTAAACCTTTTGACCGTGTAGTGGTTACCGGTCGTTTTAATGCTATTGGCGAAGCGGTGAAAGCCGTTTCTCGTCGCTCAGATAAAGAAGGTgccgcc                                                                                                                      <  1:1762684/140‑1 (MQ=255)
                  ttAACGCAGCAACAGAACTGACACCGGAGCAAGCGGCAGCGGTTAAACCTTTTGACCGTGTAGTGGTTACCGGTCGTTTTAATGCTATTGGCGAAGCGGTGAAAGCCGTTTCTCGTCGCTCAGATAAAGAAGGTgccgcc                                                                                                                      <  2:339268/140‑1 (MQ=255)
                   tAACGCAGCAACAGAACTGACACCGGAGCAAGCGGCAGCGGTTAAACCTTTTGACCGTGTAGTGGTTACCGGTCGTTTTAATGCTATTGGCGAAGCGGTGAAAGCCGTTTCTCGTCGCTCAGATAAAGAAGGTGCCGCct                                                                                                                     >  1:1114556/1‑140 (MQ=255)
                    aaCGCAGCAACAGAACTGACACCGGAGCAAGCGGCAGCGGTTAAACCTTTTGACCGTGTAGTGGTTACCGGTCGTTTTAATGCTATTGGCGAAGCGGTGAAAGCCGTTTCTCGTCGCTCAGATAAAGAAGGTGCCGCctc                                                                                                                    <  2:482943/140‑1 (MQ=255)
                        cagcaACAGAACTGACACCGGAGCAAGCGGCAGCGGTTAAACCTTTTGACCGTGTAGTGGTTACCGGTCGTTTTAATGCTATTGGTGAAGCGGTGAAAGCCGTTTCTCGTCGCTCAGATAAAGAAGGTGCCGCCTCtttt                                                                                                                >  1:754267/1‑140 (MQ=255)
                        cagcaACAGAACTGACACCGGAGCAAGCGGCAGCGGTTAAACCTTTTGACCGTGTAGTGGTTACCGGTCGTTTTAATGCTATTGGCGAAGCGGTGAAAGCCGTTTCTCGTCGCTCAGATAAAGAAGGTGCCGCCTCtttt                                                                                                                >  1:445356/1‑140 (MQ=255)
                         agcaACAGAACTGACACCGGAGCAAGCGGCAGCGGTTAAACCTTTTGACCGTGTAGTGGTTACCGGTCGTTTTAATGCTATTGGCGAAGCGGTGAAAGCCGTTTCTCGTCGCTCAGATAAAGAAGGTGCCGCCTCttttt                                                                                                               <  1:495796/140‑1 (MQ=255)
                         agcaACAGAACTGACACCGGAGCAAGCGGCAGCGGTTAAACCTTTTGACCGTGTAGTGGTTACCGGTCGTTTTAATGCTATTGGCGAAGCGGTGAAAGCCGTTTCTCGTCGCTCAGATAAAGAAGGTGCCGCCTCttttt                                                                                                               <  2:594544/140‑1 (MQ=255)
                            aaCAGAACTGACACCGGAGCAAGCGGCAGCGGTTAAACCTTTTGACCGTGTAGTGGTTACCGGTCGTTTTAATGCTATTGGCGAAGCGGTGAAAGCCGTTTCTCGTCGCTCAGATAAAGAAGGTGCCGCCTCTTTTTAtg                                                                                                            <  2:1856279/140‑1 (MQ=255)
                              cAGAACTGACACCGGAGCAAGCGGCAGCGGTTAAACCTTTTGACCGTGTAGTGGTTACCGGTCGTTTTAATGCTATTGGCGAAGCGGTGAAAGCCGTTTCTCGTCGCTCAGATAAAGAAGGTGCCGCCTCTTTTTAtgtt                                                                                                          <  2:1155844/140‑1 (MQ=255)
                                   cTGACACCGGAGCAAGCGGCAGCGGTTAAACCTTTTGACCGTGTAGTGGTTACCGGTCGTTTTAATGCTATTGGCGAAGCGGTGAAAGCCGTTTCTCGTCGCTCAGATAAAGAAGGTGCCGCCTCTTTTTATGTTGTCGa                                                                                                     <  1:100686/140‑1 (MQ=255)
                                   cTGACACCGGAGCAAGCGGCAGCGGTTAAACCTTTTGACCGTGTAGTGGTTACCGGTCGTTTTAATGCTATTGGCGAAGCGGTGAAAGCCGTTTCTCGTCGCTCAGATAAAGAAGGTGCCGCCTCTTTTTATGTTGTCGa                                                                                                     >  2:304287/1‑140 (MQ=255)
                                   cTGACACCGGAGCAAGCGGCAGCGGTTAAACCTTTTGACCGTGTAGTGGTTACCGGTCGTTTTAATGCTATTGGCGAAGCGGTGAAAGCCGTTTCTCGTCGCTCAGATAAAGAAGGTGCCGCCTCTTTTTATGTTGTCGa                                                                                                     <  1:553473/140‑1 (MQ=255)
                                   cTGACACCGGAGCAAGCGGCAGCGGTTAAACCTTTTGACCGTGTAGTGGTTACCGGTCGTTTTAATGCTATTGGCGAAGCGGTGAAAGCCGTTTCTCGTCGCTCAGATAAAGAAGGTGCCGCCTCTTTTTATGTTGTCGa                                                                                                     >  2:852223/1‑140 (MQ=255)
                                   cTGACACCGGAGCAAGCGGCAGCGGTTAAACCTTTTGACCGTGTAGTGGTTACCGGTCGTTTTAATGCTATTGGCGAAGCGGTGAAAGCCGTTTCTCGTCGCTCAGATAAAGAAGGTGCCGCCTCTTTTTATGTTGTCGa                                                                                                     <  1:1338197/140‑1 (MQ=255)
                                         ccGGAGCAAGCGGCAGCGGTTAAACCTTTTGACCGTGTAGTGGTTACCGGTCGTTTTAATGCTATTGGCGAAGCGGTGAAAGCCGTTTCTCGTCGCTCAGATAAAGAAGGTGCCGCCTCTTTTTATGTTGTCGACACTTc                                                                                               <  1:1065048/140‑1 (MQ=255)
                                           ggAGCAAGCGGCAGCGGTTAAACCTTTTGACCGTGTAGTGGTTACCGGTCGTTTTAATGCTATTGGCGAAGCGGTGAAAGCCGTTTCTCGTCGCTCAGATAAAGAAGGTGCCGCCTCTTTTTATGTTGTCGACACTTCTg                                                                                             >  2:1304225/1‑140 (MQ=255)
                                           ggAGCAAGCGGCAGCGGTTAAACCTTTTGACCGTGTAGTGGTTACCGGTCGTTTTAATGCTATTGGCGAAGCGGTGAAAGCCGTTTCTCGTCGCTCAGATAAAGAAGGTGCCGCCTCTTTTTATGTTGTCGACACTTCTg                                                                                             <  2:164212/140‑1 (MQ=255)
                                           ggAGCAAGCGGCAGCGGTTAAACCTTTTGACCGTGTAGTGGTTACCGGTCGTTTTAATGCTATTGGCGAAGCGGTGAAAGCCGTTTCTCGTCGCTCAGATAAAGAAGGTGCCGCCTCTTTTTATGTTGTCGACACTTCTg                                                                                             >  1:1767152/1‑140 (MQ=255)
                                           ggAGCAAGCGGCAGCGATTAAACCTTTTGACCGTGTAGTGGTTACCGGTCGTTTTAATGCTATTGGCGAAGCGGTGAAAGCCGTTTCTCGTCGCTCAGATAAAGAAGGTGCCGCCTCTTTTTATGTTGTCGACACTTCTg                                                                                             >  2:1408563/1‑140 (MQ=255)
                                             aGCAAGCGGCAGCGGTTAAACCTTTTGACCGTGTAGTGGTTACCGGTCGTTTTAATGCTATTGGCGAAGCGGTGAAAGCCGTTTCTCGTCGCTCAGATAAAGAAGGTGCCGCCTCTTTTTATGTTGTCGACACTTCTGAt                                                                                           <  2:2010158/140‑1 (MQ=255)
                                                aaGCGGCAGCGGTTAAACCTTTTGACCGTGTAGTGGTTACCGGTCGTTTTAATGCTATTGGCGAAGCGGTGAAAGCCGTTTCTCGTCGCTCAGATAAAGAAGGTGCCGCCTCTTTTTATGTTGTCGACACTTCTGAtttt                                                                                        <  2:1114556/140‑1 (MQ=255)
                                                  gCGGCAGCGGTTAAACCTTTTGACCGTGTAGTGGTTACCGGTCGTTTTAATGCTATTGGCGAAGCGGTGAAAGCCGTTTCTCGTCGCTCAGATAAAGAAGGTGCCGCCTCTTTTTATGTTGTCGACACTTCTGATTTTgg                                                                                      >  1:122040/1‑140 (MQ=255)
                                                  gCGGCAGCGGTT‑AACCTTTTGACCGTGTAGTGGTTACCGGTCGTTTTAATGCTATTGGCGAAGCGGTGAAAGCCGTTTCTCGTCGCTCAGATAAAGAAGGTGCCGCCTCTTTTTATGTTGTCGACACTTCTGATTTTGGt                                                                                     <  1:1020132/140‑1 (MQ=255)
                                                   cGGCAGCGGTTAAACCTTTTGACCGTGTAGTGGTTACCGGTCGTTTTAATGCTATTGGCGAAGCGGTGAAAGCCGTTTCTCGTCGCTCAGATAAAGAAGGTGCCGCCTCTTTTTATGTTGTCGACACTTCTGATTTTGGt                                                                                     <  1:963259/140‑1 (MQ=255)
                                                    ggCAGCGGTTAAACCTTTTGACCGTGTAGTGGTTACCGGTCGTTTTAATGCTATTGGCGAAGCGGTGAAAGCCGTTTCTCGTCGC‑TAGATAAAGAAGGTGCCGCCTCTTTTTATGTTGTCGACACTTCTGATTTTGGTaa                                                                                   <  2:1195504/140‑1 (MQ=255)
                                                     gCAGCGGTTAAACCTTTTGACCGTGTAGTGGTTACCGGTCGTTTTAATGCTATTGGCGAAGCGGTGAAAGCCGTTTCTCGTCGCTCAGATAAAGAAGGTGCCGCCTCTTTTTATGTTGTCGACACTTCTGATTTTGGTaa                                                                                   <  2:1763250/140‑1 (MQ=255)
                                                      cAGCGGTTAAACCTTTTGACCGTGTAGTGGTTACCGGTCGTTTTAATGCTATTGGCGAAGCGGTGAAAGCCGTTTCTCGTCGCTCAGATAAAGAAGGTGCCGCCTCTTTTTATGTTGTCGACACTTCTGATTTTGGTAAc                                                                                  <  1:1096659/140‑1 (MQ=255)
                                                       aGCGGTTAAACCTTTTGACCGTGTAGTGGTTACCGGTCGTTTTAATGCTATTGGCGAAGCGGTGAAAGCCGTTTCTCGTCGCTCAGATAAAGAAGGTGCCGCCTCTTTTTATGTTGTCGACACTTCTGATTTTGGTAACa                                                                                 <  2:1853101/140‑1 (MQ=255)
                                                                  cTTTTGACCGTGTAGTGGTTACCGGTCGTTTTAATGCTATTGGCGAAGCGGTGAAAGCCGTTTCTCGTCGCTCAGATAAAGAAGGTGCCGCCTCTTTTTATGTTGTCGACACTTCTGATTTTGGTAACAGCGGTAACTgg                                                                      <  2:2162594/140‑1 (MQ=255)
                                                                   ttttGACCGTGTAGTGGTTACCGGTCGTTTTAATGCTATTGGCGAAGCGGTGAAAGCCGTTTCTCGTCGCTCAGATAAAGAAGGTGCCGCCTCTTTTTATGTTGTCGACACTTCTGATTTTGGTAACAGCGGTAACTGGc                                                                     <  1:1730160/140‑1 (MQ=255)
                                                                     ttGACCGTGTAGTGGTTACCGGTCGTTTTAATGCTATTGGCGAAGCGGTGAAAGCCGTTTCTCGTCGCTCAGATAAAGAAGGTGCCGCCTCTTTTTATGTTGTCGACACTTCTGATTTTGGTAACAGCGGTAAc                                                                         <  1:628317/134‑1 (MQ=255)
                                                                     ttGACCGTGTAGTGGTTACCGGTCGTTTTAATGCTATTGGCGAAGCGGTGAAAGCCGTTTCTCGTCGCTCAGATAAAGAAGGTGCCGCCTCTTTTTATGTTGTCGACACTTCTGATTTTGGTAACAGCGGTAAc                                                                         >  2:628317/1‑134 (MQ=255)
                                                                     ttGACCGTGTAGTGGTTACCGGTCGTTTTAATGCTATTGGCGAAGCGGTGAAAGCCGTTTCTCGTCGCTCAGATAAAGAAGGTGCCGCCTCTTTTTATGTTGTCGACACTTCTGATTTTGGTAACAGCGGTAACTGGCgt                                                                   >  2:1063422/1‑140 (MQ=255)
                                                                     ttGACCGTGTAGTGGTTACCGGTCGTTTTAATGCTATTGGCGAAGCGGTGAAAGCCGTTTCTCGTCGCTCAGATAAAGAAGGTGCCGCCTCTTTTTATGTTGTCGACACTTCTGATTTTGGTAACAGCGGTAACTGGCgt                                                                   >  2:831856/1‑140 (MQ=255)
                                                                     ttGACCGTGTAGTGGTTACCGGTCGTTTTAATGCTATTGGCGAAGCGGTGAAAGCCGTTTCTCGTCGCTCAGATAAAGAAGGTGCCGCCTCTTTTTATGTTGTCGACACTTCTGATTTTGGTAACAGCGGTAACTGGCgt                                                                   >  1:886300/1‑140 (MQ=255)
                                                                       gACCGTGTAGTGGTTACCGGTCGTTTTAATGCTATTGGCGAAGCGGTGAAAGCCGTTTCTCGTCGCTCAGATAAAGAAGGTGCCGCCTCTTTTTATGTTGTCGACACTTCTGATTTTGGTAACAGCGGTAACTGGCgtgt                                                                 <  1:1719290/140‑1 (MQ=255)
                                                                       gACCGTGTAGTGGTTACCGGTCGTTTTAATGCTATTGGCGAAGCGGTGAAAGCCGTTTCTCGTCGCTCAGATAAAGAAGGTGCCGCCTCTTTTTATGTTGTCGACACTTCTGATTTTGGTAACAGCGGTAACTGGCgtgt                                                                 <  1:1860899/140‑1 (MQ=255)
                                                                            tgtAGTGGTTACCGGTCGTTTTAATGCTATTGGCGAAGCGGTGAAAGCCGTTTCTCGTCGCTCAGATAAAGAAGGTGCCGCCTCTTTTTATGTTGTCGACACTTCTGATTTTGGTAACAGCGGTAACTGGCGTGTGGTCg                                                            >  2:175542/1‑140 (MQ=255)
                                                                              tAGTGGTTACCGGTCGTTTTAATGCTATTGGCGAAGCGGTGAAAGCCGTTTCTCGTCGCTCAGATAAAGAAGGTGCCGCCTCTTTTTATGTTGTCGACACTTCTGATTTTGGTAACAGCGGTAACTGGCGTGTGGTCGCt                                                          <  2:515858/140‑1 (MQ=255)
                                                                                 tGGTTACCGGTCGTTTTAATGCTATTGGCGAAGCGGTGAAAGCCGTTTCTCGTCGCTCAGATAAAGAAGGTGCCGCCTCTTTTTATGTTGTCGACACTTCTGATTTTGGTAACAGCGGTAACTGGCGTGTGGTCGCTGAc                                                       <  1:94116/140‑1 (MQ=255)
                                                                                 tGGTTACCGGTCGTTTTAATGCTATTGGCGAAGCGGTGAAAGCCGTTTCTCGTCGCTCAGATAAAGAAGGTGCCGCCTCTTTTTATGTTGTCGACACTTCTGATTTTGGTAACAGCGGTAACTGGCGTGTGGTCGCTGAc                                                       <  1:1274662/140‑1 (MQ=255)
                                                                                 tGGTTACCGGTCGTTTTAATGCTATTGGCGAAGCGGTGAAAGCCGTTTCTCGTCGCTCAGATAAAGAAGGTGCCGCCTCTTTTTATGTTGTCGACACTTCTGATTTTGGTAACAGCGGTAACTGGCGTGTGGTCGCTGAc                                                       <  1:1925469/140‑1 (MQ=255)
                                                                                 tGGTTACCGGTCGTTTTAATGCTATTGGCGAAGCGGTGAAAGCCGTTTCTCGTCGCTCAGATAAAGAAGGTGCCGCCTCTTTTTATGTTGTCGACACTTCTGATTTTGGTAACAGCGGTAACTGGCGTGTGGTCGCTGAc                                                       <  1:1870003/140‑1 (MQ=255)
                                                                                  ggTTACCGGTCGTTTTAATGCTATTGGCGAAGCGGTGAAAGCCGTTTCTCGTCGCTCAGATAAAGAAGGTGCCGCCTCTTTTTATGTTGTCGACACTTCTGATTTTGGTAACAGCGGTAACTGGCGTGTGGTCGCTGAcc                                                      <  1:1640082/140‑1 (MQ=255)
                                                                                  ggTTACCGGTCGTTTTAATGCTATTGGCGAAGCGGTGAAAGCCGTTTCTCGTCGCTCAGATAAAGAAGGTGCCGCCTCTTTTTATGTTGTCGACACTTCTGATTTTGGTAACAGCGGTAACTGGCGTGTGGTCGCTGAcc                                                      <  2:445356/140‑1 (MQ=255)
                                                                                  ggTTACCGGTCGTTTTAATGCTATTGGCGAAGCGGTGAAAGCCGTTTCTCGTCGCTCAGATAAAGAAGGTGCCGCCTCTTTTTATGTTGTCGACACTTCTGATTTTGGTAACAGCGGTAACTGGCGTGTGGTCGCTGAcc                                                      <  2:1208227/140‑1 (MQ=255)
                                                                                   gTTACCGGTCGTTTTAATGCTATTGGCGAAGCGGTGAAAGCCGTTTCTCGTCGCTCAGATAAAGAAGGTGCCGCCTCTTTTTATGTTGTCGACACTTCTGATTTTGGTAACAGCGGTAACTGGCGTGTGGTCGCTGAc                                                       <  1:1407018/138‑1 (MQ=255)
                                                                                   gTTACCGGTCGTTTTAATGCTATTGGCGAAGCGGTGAAAGCCGTTTCTCGTCGCTCAGATAAAGAAGGTGCCGCCTCTTTTTATGTTGTCGACACTTCTGATTTTGGTAACAGCGGTAACTGGCGTGTGGTCGCTGAc                                                       <  2:342906/138‑1 (MQ=255)
                                                                                   gTTACCGGTCGTTTTAATGCTATTGGCGAAGCGGTGAAAGCCGTTTCTCGTCGCTCAGATAAAGAAGGTGCCGCCTCTTTTTATGTTGTCGACACTTCTGATTTTGGTAACAGCGGTAACTGGCGTGTGGTCGCTGAc                                                       >  1:342906/1‑138 (MQ=255)
                                                                                   gTTACCGGTCGTTTTAATGCTATTGGCGAAGCGGTGAAAGCCGTTTCTCGTCGCTCAGATAAAGAAGGTGCCGCCTCTTTTTATGTTGTCGACACTTCTGATTTTGGTAACAGCGGTAACTGGCGTGTGGTCGCTGACct                                                     >  2:1282737/1‑140 (MQ=255)
                                                                                   gTTACCGGTCGTTTTAATGCTATTGGCGAAGCGGTGAAAGCCGTTTCTCGTCGCTCAGATAAAGAAGGTGCCGCCTCTTTTTATGTTGTCGACACTTCTGATTTTGGTAACAGCGGTAACTGGCGTGTGGTCGCTGACct                                                     >  1:275047/1‑140 (MQ=255)
                                                                                      aCCGGTCGTTTTAATGCTATTGGCGAAGCGGTGAAAGCCGTTTCTCGTCGCTCAGATAAAGAAGGTGCCGCCTCTTTTTATGTTGTCGACACTTCTGATTTTGGTAACAGCGGTAACTGGCGTGTGGTCg                                                            <  1:2043681/130‑1 (MQ=255)
                                                                                      aCCGGTCGTTTTAATGCTATTGGCGAAGCGGTGAAAGCCGTTTCTCGTCGCTCAGATAAAGAAGGTGCCGCCTCTTTTTATGTTGTCGACACTTCTGATTTTGGTAACAGCGGTAACTGGCGTGTGGTCg                                                            >  2:2043681/1‑130 (MQ=255)
                                                                                      aCCGGTCGTTTTAATGCTATTGGCGAAGCGGTGAAAGCCGTTTCTCGTCGCTCAGATAAAGAAGGTGCCGCCTCTTTTTATGTTGTCGACACTTCTGATTTTGGTAACAGCGGTAACTGGCGTGTGGTCGCTGACCTCta                                                  >  2:1420322/1‑140 (MQ=255)
                                                                                      aCCGGTCGTTTTAATGCTATTGGCGAAGCGGTGAAAGCCGTTTCTCGTCGCTCAGATAAAGAAGGTGCCGCCTCTTTTTATGTTGTCGACACTTCTGATTTTGGTAACAGCGGTAACTGGCGTGTGGTCGCTGACCTCta                                                  >  1:1191440/1‑140 (MQ=255)
                                                                                      aCCGGTCGTTTTAATGCTATTGGCGAAGCGGTGAAAGCCGTTTCTCGTCGCTCAGATAAAGAAGGTGCCGCCTCTTTTTATGTTGTCGACACTTCTGATTTTGGTAACAGCGGTAACTGGCGTGTGGTCGCTGACCTCta                                                  >  2:758523/1‑140 (MQ=255)
                                                                                      aCCGGTCGTTTTAATGCTATTGGCGAAGCGGTGAAAGCCGTTTCTCGTCGCTCAGATAAAGAAGGTGCCGCCTCTTTTTATGTTGTCGACACTTCTGATTTTGGTAACAGCGGTAACTGGCGTGTGGTCGCTGACCTCta                                                  >  1:779052/1‑140 (MQ=255)
                                                                                      aCCGGTCGTTTTAATGCTATTGGCGAAGCGGTGAAAGCCGTTTCTCGTCGCTCAGATAAAGAAGGTGCCGCCTCTTTTTATGTTGTCGACACTTCTGATTTTGGTAACAGCGGTAACTGGCGTGTGGTCGCTGACCTCta                                                  >  1:1611551/1‑140 (MQ=255)
                                                                                           tCGTTTTAATGCTATTGGCGAAGCGGTGAAAGCCGTTTCTCGTCGCTCAGATAAAGAAGGTGCCGCCTCTTTTTATGTTGTCGACACTTCTGATTTTGGTAACAGCGGTAACTGGCGTGTGGTCGCTGAc                                                       <  1:875160/130‑1 (MQ=255)
                                                                                           tCGTTTTAATGCTATTGGCGAAGCGGTGAAAGCCGTTTCTCGTCGCTCAGATAAAGAAGGTGCCGCCTCTTTTTATGTTGTCGACACTTCTGATTTTGGTAACAGCGGTAACTGGCGTGTGGTCGCTGAc                                                       >  2:875160/1‑130 (MQ=255)
                                                                                           tCGTTTTAATGCTATTGGCGAAGCGGTGAAAGCCGTTTCTCGTCGCTCAGATAAAGAAGGTGCCGCCTCTTTTTATGTTGTCGACACTTCTGATTTTGGTAACAGCGGTAACTGGCGTGTGGTCGCTGACCTCTATAAAg                                             >  1:1268091/1‑140 (MQ=255)
                                                                                           tCGTTTTAATGCTATTGGCGAAGCGGTGAAAGCCGTTTCTCGTCGCTCAGATAAAGAAGGTGCCGCCTCTTTTTATGTTGTCGACACTTCTGATTTTGGTAACAGCGGTAACTGGCGTGTGGTCGCTGACCTCTATAAAg                                             >  2:1564795/1‑140 (MQ=255)
                                                                                           tCGTTTTAATGCTATTGGCGAAGCGGTGAAAGCCGTTTCTCGTCGCTCAGATAAAGAAGGTGCCGCCTCTTTTTATGTTGTCGACACTTCTGATTTTGGTAACAGCGGTAACTGGCGTGTGGTCGCTGACCTCTATAAAg                                             >  2:535202/1‑140 (MQ=255)
                                                                                           tCGTTTTAATGCTATTGGCGAAGCGGTGAAAGCCGTTTCTCGTCGCTCAGATAAAGAAGGTGCCGCCTCTTTTTATGTTGTCGACACTTCTGA‑TTTGGTAACAGCGGTAACTGGCGTGTGGTCGCTGACCTCTATAAAGc                                            <  1:2205805/140‑1 (MQ=255)
                                                                                             gTTTTAATGCTATTGGCGAAGCGGTGAAAGCCGTTTCTCGTCGCTCAGATAAAGAAGGTGCCGCCTCTTTTTATGTTGTCGACACTTCTGATTTTGGTAACAGCGGTAACTGGCGTGTGGTCGCt                                                          <  1:471843/125‑1 (MQ=255)
                                                                                             gTTTTAATGCTATTGGCGAAGCGGTGAAAGCCGTTTCTCGTCGCTCAGATAAAGAAGGTGCCGCCTCTTTTTATGTTGTCGACACTTCTGATTTTGGTAACAGCGGTAACTGGCGTGTGGTCGCt                                                          >  2:471843/1‑125 (MQ=255)
                                                                                                         ttGGCGAAGCGGTGAAAGCCGTTTCTCGTCGCTCAGATAAAGAAGGTGCCGCCTCTTTTTATGTTGTCGACACTTCTGATTTTGGTAACAGCGGTAACTGGCGTGTGGTCGCTGACCTCtat                                                 >  2:1733907/1‑122 (MQ=255)
                                                                                                         ttGGCGAAGCGGTGAAAGCCGTTTCTCGTCGCTCAGATAAAGAAGGTGCCGCCTCTTTTTATGTTGTCGACACTTCTGATTTTGGTAACAGCGGTAACTGGCGTGTGGTCGCTGACCTCTATAAAGCCGATGCTGaaaaa                               >  1:1843513/1‑140 (MQ=255)
                                                                                                         ttGGCGAAGCGGTGAAAGCCGTTTCTCGTCGCTCAGATAAAGAAGGTGCCGCCTCTTTTTATGTTGTCGACACTTCTGATTTTGGTAACAGCGGTAACTGGCGTGTGGTCGCTGACCTCTATAAAGCCGATGCTGaaaaa                               <  1:1911054/140‑1 (MQ=255)
                                                                                                          tGGCGAAGCGGTGAAAGCCGTTTCTCGTCGCTCAGATAAAGAAGGTGCCGCCTCTTTTTATGTTGTCGACACTTCTGATTTTGGTAACAGCGGTAACTGGCGTGTGGTCGCTGACCTCTATAAAGCCGATGCTGAAAAAg                              <  1:637997/140‑1 (MQ=255)
                                                                                                          tGGCGAAGCGGTGAAAGCCGTTTCTCGTCGCTCAGATAAAGAAGGTGCCGCCTCTTTTTATGTTGTCGACACTTCTGATTTTGGTAACAGCGGTAACTGGCGTGTGGTCGCTGACCTCTATAAAGCCGATGCTGAAAAAg                              <  1:535202/140‑1 (MQ=255)
                                                                                                           ggCGAAGCGGTGAAAGCCGTTTCTCGTCGCTCAGATAAAGAAGGTGCCGCCTCTTTTTATGTTGTCGACACTTCTGATTTTGGTAACAGCGGTAACTGGCGTGTGGTCGCTGACCTCTATAAAGCCGATGCTGAAAAAGc                             >  2:1941241/1‑140 (MQ=255)
                                                                                                              gAAGCGGTGAAAGCCGTTTCTCGTCGCTCAGATAAAGAAGGTGCCGCCTCTTTTTATGTTGTCGACACTTCTGATTTTGGTAACAGCGGTAACTGGCGTGTGGTCGCTGACCTCTATAAAGCCGATGCTGAAAAAGCaga                          <  1:1452655/140‑1 (MQ=255)
                                                                                                                aGCGGTGAAAGCCGTTTCTCGTCGCTCAGATAAAGAAGGTGCCGCCTCTTTTTATGTTGTCGACACTTCTGATTTTGGTAACAGCGGTAACTGGCGTGTGGTCGCTGACCTCTATAAAGCCGATGCTGAAAAAGCagaag                        <  2:76144/140‑1 (MQ=255)
                                                                                                                aGCGGTGAAAGCCGTTTCTCGTCGCTCAGATAAAGAAGGTGCCGCCTCTTTTTATGTTGTCGACACTTCTGATTTTGGTAACAGCGGTAACTGGCGTGTGGTCGCTGACCTCTATAAAGCCGATGCTGAAAAAGCagaag                        <  1:249072/140‑1 (MQ=255)
                                                                                                                 gCGGTGAAAGCCGTTTCTCGTCGCTCAGATAAAGAAGGTGCCGCCTCTTTTTATGTTGTCGACACTTCTGATTTTGGTAACAGCGGTAACTGGCGTGTGGTCGCTGACCTCTATAAAGCCGATGCTGAAAAAGCagaaga                       >  2:359944/1‑140 (MQ=255)
                                                                                                                   ggTGAAAGCCGTTTCTCGTCGCTCAGATAAAGAAGGTGCCGCCTCTTTTTATGTTGTCGACACTTCTGATTTTGGTAACAGCGGTAACTGGCGTGTGGTCGCTGACCTCTATAAAGCCGATGCTGAAAAAGCagaag                        <  2:307102/137‑1 (MQ=255)
                                                                                                                         aGCCGTTTCTCGTCGCTCAGATAAAGAAGGTGCCGCCTCTTTTTATGTTGTCGACACTTCTGATTTTGGTAACAGCGGTAACTGGCGTGTGGTCGCTGACCTCTATAAAGCCGATGCTGAAAAAGCAGAAGAAACAAGTa               <  1:943062/140‑1 (MQ=255)
                                                                                                                              tttCTCGTCGCTCAGATAAAGAAGGTGCCGCCTCTTTTTATGTTGTCGACACTTCTGATTTTGGTAACAGCGGTAACTGGCGTGTGGTCGCTGACCTCTATAAAGCCGATGCTGAAAAAGCAGAAGAAACAAGTAATcgc          <  1:1282737/140‑1 (MQ=255)
                                                                                                                              tttCTCGTCGCTCAGATAAAGAAGGTGCCGCCTCTTTTTATGTTGTCGACACTTCTGATTTTGGTAACAGCGGTAACTGGCGTGTGGTCGCTGACCTCTATAAAGCCGATGCTGAAAAAGCAGAAGAAACAAGTAATcgc          <  2:559831/140‑1 (MQ=255)
                                                                                                                              tttCTCGTCGCTCAGATAAAGAAGGTGCCGCCTCTTTTTATGTTGTCGACACTTCTGATTTTGGTAACAGCGGTAACTGGCGTGTGGTCGCTGACCTCTATAAAGCCGATGCTGAAAAAGCAGAAGAAACAAGTAATcgc          <  1:1061737/140‑1 (MQ=255)
                                                                                                                                         |                                                                                                                                        
CTGCTATGGCATTCTCCGTTAACGCAGCAACAGAACTGACACCGGAGCAAGCGGCAGCGGTTAAACCTTTTGACCGTGTAGTGGTTACCGGTCGTTTTAATGCTATTGGCGAAGCGGTGAAAGCCGTTTCTCGTCGCGCAGATAAAGAAGGTGCCGCCTCTTTTTATGTTGTCGACACTTCTGATTTTGGTAACAGCGGTAACTGGCGTGTGGTCGCTGACCTCTATAAAGCCGATGCTGAAAAAGCAGAAGAAACAAGTAATCGCGTAATTAA  >  NC_000913/1678467‑1678740

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑