Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913445,5580AC36.4% 50.4 / 10.3 33V370G (GTA→GGA) yajRputative major facilitator superfamily transport protein YajR
Reads supporting (aligned to +/- strand):  ref base A (16/5);  new base C (0/12);  total (16/17)
Fisher's exact test for biased strand distribution p-value = 2.26e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

AGCAAACGCACTTTTAACGCCTCGTTTGCGGCAATGTTCGCCGGGATTTCAATGCGCAAACTGCTGACATACGGCGGTTCTTTCATGGTACTGGCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGACCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTGGCGACTCTTTACTGATAA  >  NC_000913/445420‑445690
                                                                                                                                          |                                                                                                                                    
aGCAAACGCACTTTTAACGCCTCGTTTGCGGCAATGTTCGCCGGGATTTCAATGCGCAAACTGCTGACATACGGCGGTTCTTTCATGGTACTGGCGACTGTCAGCCACACTGCGGCCCGCATTGCGCCAGCGAGGAATAc                                                                                                                                     >  2:17481/1‑140 (MQ=255)
     aCGCACTTTTAACGCCTCGTTTGCGGCAATGTTCGCCGGGATTTCAATGCGCAAACTGCTGACATACGGCGGTTCTTTCATGGTACTGGCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCt                                                                                                                                <  2:1206289/140‑1 (MQ=255)
               aaCGCCTCGTTTGCGGCAATGTTCGCCGGGATTTCAATGCGCAAACTGCTGACATACGGCGGTTCTTTCATGGTACTGGCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGACCGTCaaa                                                                                                                      <  1:1139320/140‑1 (MQ=255)
                  gCCTCGTTTGCGGCAATGTTCGCCGGGATTTCAATGCGCAAACTGCTGACATACGGCGGTTCTTTCATGGTACTGGCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGACCGTCAAACAt                                                                                                                   <  1:312746/140‑1 (MQ=255)
                     tCGTTTGCGGCAATGTTCGCCGGGATTTCAATGCGCAAACTGCTGACATACGGCGGCTCTTTCCTGGTAATGGCGACCGTCCACCCCACCGAGGACGGCATTAGGCCAACCAGAAATAACCCCCGACCGTCAAACATGcc                                                                                                                >  2:149175/1‑140 (MQ=255)
                               cAATGTTCGCCGGGATTTCAATGCGCAAACTGCTGACATACGGCGGTTCTTTCATGGTACTGGCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGACCGTCAAACATGCCGTTAATCCAg                                                                                                      <  1:370131/140‑1 (MQ=255)
                                  tGTTCGCCGGGATTTCAATGCGCAAACTGCTGACATACGGCGGGTTTTTTATGGTATTTGCGACTGTCAACCCCACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGACCGTCAAACATGCCGTTAATCCCgccc                                                                                                   >  2:694475/1‑139 (MQ=255)
                                                      cgcAAACTGCTGACATACGGCGGTTCTTTCATGGTACTGGCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGACCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATcgc                                                                               >  1:774026/1‑140 (MQ=255)
                                                      cgcAAACTGCTGACATACGGCGGTTCTTTCATGGTACTGGCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCACCGAGGAAAACCCCCCGCCCGTCAAACATACCGTTAAACCATCCGCCCAGCGAACCCCCAATcgc                                                                               >  2:422524/1‑140 (MQ=255)
                                                       gcAAACTGCTGACATACGGCGGTTCTTTCATGGTACTGGCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGACCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATcgcc                                                                              >  1:102221/1‑140 (MQ=255)
                                                        cAAACTGCTGACATACGGCGGTTCTTTCATGGTACTGGCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATCCCCCCTGACCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATcgcca                                                                             <  1:1145246/140‑1 (MQ=255)
                                                        aaaacacctgACATGCGGCGGTCCTTTCATGGTACTGGCGACTGTCAGCCACACTGGGGCCAGCATTGCGCCAGGGAGAAATCCCCCCTGCCCTTCAAACATGCCGTTAACCCAGCCGCCCAGCGAACCGCCAATcgcca                                                                             <  2:561101/133‑1 (MQ=255)
                                                        aaaaCTGCGGACATAGGGCGGTTCTTTGGGGGTACTGGCGATTGCCACCCACCCTGCGGCCACCATCGCGCCAGCGAGAAATCCCCCTTGCCCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATcgcca                                                                             <  2:1113836/139‑1 (MQ=255)
                                                         aaaCTGCTGACATACGGCGGTTCTTTCATGGTACTGGCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCCGACCGTCAAACATTCCGTTAATCCAGCCGCCCAGCGAACCCCCAAAcgccac                                                                            >  2:106425/1‑140 (MQ=255)
                                                                tgACATACGGCGGTTCTTTTATGGTACTGGCGACTGTCAGCCCCACTGCGGCCAGCATTTCGCCCGCGAGAAAAACCCCCTGACCGTCAAACATGCCGTTAATCCAGCCCCCCAGCGAACCCCCAATCGCCCCGCCAAGa                                                                     >  2:436846/1‑140 (MQ=255)
                                                                  aCATCCGGCGTTTTTTTCATGGTCCGGGCGCCTGCCAGCCACACTGCGGCAAGAATGGCGCCAGCGAAAAATCCCCCCTGCCCGTAAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGaaa                                                                   <  2:984804/140‑1 (MQ=255)
                                                                    aTACGGCGGTTCTTTCATGGTACTGGCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATCCCCCCTGCCCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAAct                                                                 <  1:521756/140‑1 (MQ=255)
                                                                       cggggtttctttctTGGTCCGGCCGATTGCCAGCCACACTGGGGCCAGATTTGCGCCAGCGAGAAATCCCCCCTGCCCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAActggc                                                              <  2:1284628/134‑1 (MQ=255)
                                                                       cggcggTTCTTTCATGGTACTGGCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATCCCCCCTGACCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAActggc                                                              <  1:419837/140‑1 (MQ=255)
                                                                           ggTTTTTTCATGTTTCTGGCGACGTTCGGCCACAGTGGGGGAAGCATTGCCCTATTGAGAAATCCCCCGGAACCGCCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCtggt                                                          <  2:528910/140‑1 (MQ=255)
                                                                           ggTTTTTTCATGGTACTGGCGCCTGTCAGCCACCCTGCGGCCAGCATTGCGCCAGCGAAAAATCCCCCCTGCCCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCtggt                                                          <  2:173324/140‑1 (MQ=255)
                                                                            gTTCTTTTATGGTACTGGCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATCCCCCCTGACCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCtggtg                                                         <  1:43180/140‑1 (MQ=255)
                                                                              tCTTTTATGGTACTGGCGACTGTCAGCCACACCGCGGCCAGCATTTCGCCAGCGAGAAAAAACCCCCGACCGTCAAACATGCCGTTAATCCAGCCGCCCCGCGAACCCCCAAACGCCCCCCCCAAAAAATGGCtgggggg                                                       >  2:650337/1‑139 (MQ=255)
                                                                                   cATGGTACTGGCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATCCCCCCTGACCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTaaa                                                  <  1:62696/140‑1 (MQ=255)
                                                                                       gTACTGGCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATCCCCCCTGACCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACAcc                                              <  1:547162/140‑1 (MQ=255)
                                                                                       gTACTGGCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGACCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACAcc                                              >  1:510019/1‑140 (MQ=255)
                                                                                        tccTGGCGCCTGTCACCCACCCGGCGGCCAGCTTTGCGCCAGCGAAAAATACCCCCGGACCGCAAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACAccc                                             <  2:16734/138‑1 (MQ=255)
                                                                                        tACTGGCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATCCCCCCTGACCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACAccc                                             <  1:673452/140‑1 (MQ=255)
                                                                                           tGGCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGCAATACCCCCTGACCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAAACGCCACGCCAAGAAACTGGCTGATGGAGTAAACACCCaat                                          >  2:339729/1‑138 (MQ=255)
                                                                                            ggCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGACCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCg                                         >  2:1136118/1‑140 (MQ=255)
                                                                                              cGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATCCCCCCTGACCGTCAAACATGCCGTAAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGcc                                       <  1:1214464/140‑1 (MQ=255)
                                                                                               gACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATCCCCCCTGACCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCg                                      <  1:1008905/140‑1 (MQ=255)
                                                                                                 cTGTCAGCCACACTGCGGCCAGCATTGCGCCGGCGAAAAATCCCCCCTGACCGTAAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTa                                    <  2:893793/140‑1 (MQ=255)
                                                                                                 cTGTCACCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATCCCCCCTGACCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTa                                    <  1:1141959/140‑1 (MQ=255)
                                                                                                  tGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGACCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTAc                                   >  1:1271371/1‑140 (MQ=255)
                                                                                                       gCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGACCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTg                              >  2:342963/1‑140 (MQ=255)
                                                                                                       gCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGACCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTg                              >  1:977421/1‑140 (MQ=255)
                                                                                                            acTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGACCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAAcc                         >  1:640755/1‑140 (MQ=255)
                                                                                                              tGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGACCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACctg                       >  2:961613/1‑140 (MQ=255)
                                                                                                              tGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGACCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACctg                       >  1:112667/1‑140 (MQ=255)
                                                                                                                cGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGACCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACctgct                     >  2:120873/1‑140 (MQ=255)
                                                                                                                  gCCAGCATTGCGCCAGCGAGAAATACCCCCTGACCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTgg                   >  1:513732/1‑140 (MQ=255)
                                                                                                                  gCCAGCATTGCGCCAGCGAGAAATACCCCCTGACCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTgg                   >  2:434196/1‑140 (MQ=255)
                                                                                                                   ccAGCATTGCGCCAGCGAAAAATCCCCCCTGCCCGTAAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTGGc                  <  2:816962/140‑1 (MQ=255)
                                                                                                                     aGCTTTGCGCCAGCGAAAAATCCCCCCTGACCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTGGCGa                <  2:316471/140‑1 (MQ=255)
                                                                                                                     aGCATGGCGCCAGCGAAAAATCCCCCCTGCCCGAAAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTGGCGa                <  2:640755/140‑1 (MQ=255)
                                                                                                                       cATTGCGCCAGCGAGAAATACCCCCTGACCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTGGCGAct              >  2:371506/1‑140 (MQ=255)
                                                                                                                         ttGCGCCGGCGAAAAATACCCCCTGACCGACAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTGGCGActct            <  2:119387/140‑1 (MQ=255)
                                                                                                                          tGCGCCAGCGAGAAATACCCCCTGACCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTGGCGACTCtt           >  1:1092520/1‑140 (MQ=255)
                                                                                                                           gcgcCGGCGAGAAATCCCCCCTGACCGACAAACATGCGGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTGGCGACTCttt          <  2:786123/140‑1 (MQ=255)
                                                                                                                           gcgcAAGGGAGAAATCCCCCCGGACCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTGGCGACTCttt          <  1:681785/140‑1 (MQ=255)
                                                                                                                             gcCAGCGAGAAATCCCCCTTGACCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTGGCGACTCTTTAc        <  1:149175/140‑1 (MQ=255)
                                                                                                                             cccAGCGAGAAATCCCCCCGGCCCGTAAACCATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTGGCGACTCTTTAc        <  2:11657/139‑1 (MQ=255)
                                                                                                                                gcggagaAATCCCCCCTACCCGCAAAAAATGCCGTCAATCCAGCCGCCCAGCGACCCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACCCCCATCGCCGTACCTTTGTAACCTGCTGGCGACTCTTTACTGa     <  2:1092520/137‑1 (MQ=255)
                                                                                                                                aGCGAGAAATCCCCCCTGACCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTGGCGACTCTTTACTGa     <  1:1191138/140‑1 (MQ=255)
                                                                                                                                 gCGAGAAATACCCCCTGACCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTGGCGACTCTTTACTGAt    >  1:111725/1‑140 (MQ=255)
                                                                                                                                 gCGAGAAATACCCCCTGACCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTGGCGACTCTTTACTGAt    <  1:434196/140‑1 (MQ=255)
                                                                                                                                  cGAAAAATCCCCCCTGACCGCCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTGGCGACTCTTTACTGATa   <  1:339729/140‑1 (MQ=255)
                                                                                                                                   gagaAATCCCCCCTGACCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTGGCGACTCTTTACTGATaa  <  1:1067313/140‑1 (MQ=255)
                                                                                                                                          |                                                                                                                                    
AGCAAACGCACTTTTAACGCCTCGTTTGCGGCAATGTTCGCCGGGATTTCAATGCGCAAACTGCTGACATACGGCGGTTCTTTCATGGTACTGGCGACTGTCAGCCACACTGCGGCCAGCATTGCGCCAGCGAGAAATACCCCCTGACCGTCAAACATGCCGTTAATCCAGCCGCCCAGCGAACCGCCAATCGCCACGCCAAGAAACTGGCTGGTGGAGTAAACACCCATCGCCGTACCTTTGTAACCTGCTGGCGACTCTTTACTGATAA  >  NC_000913/445420‑445690

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑