Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,800,810	C→A	L587F (TTG→TTT)	thrS ←	threonine‑‑tRNA ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,800,810	0	C	A	98.4%	221.0 / NA	64	L587F (TTG→TTT)	thrS	threonine‑‑tRNA ligase
Reads supporting (aligned to +/- strand):  ref base C (1/0);  new base A (33/30);  total (34/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01

CGAATCTCTTGTTGCAGCTTCTCGATCACTTCATTTACGTCCATGCTTCCCAGGTCTTTACCACGGCGGGTGCGAACGGCAACTTTGCCTGATTCCACCTCTTTATCACCACAGACCAGCATATATGGGACGCGACGCAAAGTGTGCTCGCGGATTTTAAAGCCAATCTTCTCATTTCTCAAGTCTGCTTTAACACGAATGCCCGCATTTGATAGTTTTTGCGTCAATTCGTTAACGTATTCAGACTGTGAATCGGTAATATTCATGATAACA  >  NC_000913/1800673‑1800945                                                                                                                                          |                                                                                                                                        cGAATCTCTTGTTGCAGCTTCTCGATCACTTCATTTACGTCCATGCTTCCCAGGTCTTTACCACGGCGGGAGCGAACGGCAACTTTGCCTGATTTCACCTCTTTATCACCACAGACCAGCATATATGGGACGCGACGaaa                                                                                                                                       >  2:579925/1‑140 (MQ=255)                 gCTTCTCGATCACTTCATTTACGTCCATGCTTCCCAGGTCTTTACCACGGCGGGTGCGAACGGCAACTTTGCCTGATTCCACCTCTTTATCACCACAGACCAGCATATATGGGACGCGACGAAAAGTGTGCTCGCGGAtt                                                                                                                       >  1:937973/1‑140 (MQ=255)                     ctcGATCACTTCATTTACGTCCATGCTTCCCAGGTCTTTACCACGGCGGGTGCGAACGGCAACTTTGCCTGATTCCACCTCTTTATCACCACAGACCAGCATATATGGGACGCGACGAAAAGTGTGCTCGCGGATTTTaa                                                                                                                   <  1:345662/140‑1 (MQ=255)                         aTCACTTCATTTACGTCCATGCTTCCCAGGTCTTTACCACGGCGGGGGCGAACGGCAAATTTGCCTTATTCCACCTCTTTATCACCACAGACCAGCATATATGGGGCGCGACCAAAAGGTTGCTCGCGGATTTTTAAGcc                                                                                                               >  2:1002565/1‑140 (MQ=255)                            aCTTCATTTACGTCCATGCTTCCCAGGTCTTTACCACGGCGGGTGCGAACGGCAACTTTGCCTGATTCCACCTCTTTATCACCACAGACCAGCATATATTGGACGCGACGAAAAGTGTGCTCGCGGATTTTAAAGCCAAt                                                                                                            >  2:691336/1‑140 (MQ=255)                                cATTTACGTCCATGCTTCCCAGGTCTTTACCACGGCGGGTGCGAACGGCAACTTTGCCTGATTCCACCTCTTTATCACCACAGACCAGCATATATGGGACGCGACGAAAAGTGTGCTCGCGGATTTTAAAGCCAAtcttc                                                                                                        >  1:544093/1‑140 (MQ=255)                                cATTTACGTCCATGCTTCCCAGGTCCTTACCACGGCGGGTGCGAACGGCAACTTTGCCTGATTCCACCTCTTTATCACCACAGACCAGCATATATGGGACGCGACGAAAAGTGTGCTCGCGGATTTTAAAGCCAAtcttc                                                                                                        >  1:1045514/1‑140 (MQ=255)                                 aTTTACGTCCATGCTTCCCAGGTCTTTACCACGGCGGGTGCGAACGGCAACTTTGCCTGATTCCACCTCTTTATCACCACAGACCAGCATATATGGGACGCGACGAAAAGTGTGCTCGCGGATTTTAAAGCCAAtcttct                                                                                                       >  2:1263500/1‑140 (MQ=255)                                 aTTTACGTCCATGCTTCCCAGGTCTTTACCACGGCGGGTGCGAACGGCAACTTTGCCTGATTCCACCTCTTTATCACCACAGACCAGCATATATGGGACGCGACGAAAAGTGTGCTCGCGGATTTTAAAGCCAAtcttct                                                                                                       <  2:100610/140‑1 (MQ=255)                                 aTTTACGTCCATGCTTCCCAGGTCTTTACCACGGCGGGTGCGAACGGCAACTTTGCCTGATTCCACCTCTTTATCACCACAGACCAGCATATATGGGACGCGACGAAAAGTGTGCTCGCGGATTTTAAAGCCAAtcttct                                                                                                       <  2:937973/140‑1 (MQ=255)                                  tttACGTCCATGCTTCCCAGGTCTTTACCACGGCGGGTGCGAACGGCAACTTTGCCTGATTCCACCTCTTTATCACCACAGACCAGCATATATGGGACGCGACGAAAAGGGTGCTCGCGGATTTTAAAGCCAAtcttccc                                                                                                      >  2:1311243/1‑138 (MQ=255)                                      cGTCCATGCTTCCCAGGTCTTTACCACGGCGTGTGCGATCGGCAACTTTGCCTGATTCCTCCTCTTTATCACCTCAGACCAGCATATATGGGACGCGACGAAAAGTGTGCTCGCGGATTTTAAAGCCAATCTTCTCAttt                                                                                                  <  2:209141/140‑1 (MQ=255)                                      cGTCCATGCTTCCCAGGTCTTTACCACGGCGGGTGCGAACGGCAACTTTGCCTGATTCCACCTCTTTATCACCACAGACCAGCATATATGGGACGCGACGAAAAGTGTGCTCGCGGATTTTAAAGCCAATCTTCTCAttt                                                                                                  >  1:1093739/1‑140 (MQ=255)                                            tGCTTCCCAGGTCTTTACCACGGCGGGTGCGAACGGCAACTTTGCCTGATTCCACCTCTTTATCACCACAGACCAGCATATATGGGACGCGACGAAAAGTGTGCTCGCGGATTTTAAAGCCAATCTTCTCATTTCTCAAg                                                                                            >  2:814694/1‑140 (MQ=255)                                            tGCTTCCCAGGTCTTTACCACGGCGGGTGCGAACGGCAACTTTGCCTGATTCCACCTCTTTATCACCACAGACCAGCATATATGGGACGCGACGAAAAGTGTGCTCGCGGATTTTAAAGCCAATCTTCTCATTTCTCAAg                                                                                            >  1:281111/1‑140 (MQ=255)                                            tGCTTCCCAGGTCTTTACCACGGCGGGTGCGAACGGCAACTTTGCCTGATTCCACCTCTTTATCACCACAGACCAGCATATATGGGACGCGACGAAAAGTGTGCTCGCGGATTTTAAAGCCAATCTTCTCATTTCTCAAg                                                                                            <  1:631528/140‑1 (MQ=255)                                                 cccAGGTCTTTACCACGGCGGGTGCGAACGGCAACTTTGCCTGATTCCACCTCTTTATCACCACAGACCAGCATATATGGGACGCGACGAAAAGTGTGCTCGCGGATTTTAAAGCCAATCTTCTCATTTCTCAAGTCTGc                                                                                       <  1:1273984/140‑1 (MQ=255)                                                       tCTTTACCACGGCGGGTGCGAACGGCAACTTTGCCTGATTCCACCTCTTTATCACCACAGACCAGCATATATGGGACGCGACGAAAAGTGTGCTCGCGGATTTTAAAGCCAATCTTCTCATTTCTCAAGTCTGCTTTAac                                                                                 >  1:288935/1‑140 (MQ=255)                                                         tttACCACGGCGGGTGCGAACGGCAACTTTGCCTGATTCCACCTCTTTATCACCACAGACCAGCATATATGGGACGCGACGAAAAGTGTGCTCGCGGATTTTAAAGCCAATCTTCTCATTTCTCAAGTCTGCTTTAacac                                                                               >  2:603789/1‑140 (MQ=255)                                                         tttACCACGGCGGGTGCGAACGGCAACTTTGCCTGATTCCACCTCTTTATCACCACAGACCAGCATATATGGGACGCGACGAAAAGTGTGCTCGCGGATTTTAAAGCCAATCTTCTCATTTCTCAAGTCTGCTTTAacac                                                                               >  1:790737/1‑140 (MQ=255)                                                           tACCACGGCGGGTGCGAACGGCAACTTTGCCTGATTCCACCTCTTTATCACCACAGACCAGCATATATGGGACGCGACGAAAAGTGTGCTCGCGGATTTTAAAGCCAATCTTCTCATTTCTCAAGTCTGCTTTAACACGa                                                                             >  2:903261/1‑140 (MQ=255)                                                           tACCACGGCGGGTGCGAACGGCAACTTTGCCTGATTCCACCTCTTTATCACCACAGACCAGCATATATGGGACGCGACGAAAAGTGTGCTCGCGGATTTTAAAGCCAATCTTCTCATTTCTCAAGTCTGCTTTAACACGa                                                                             >  1:619427/1‑140 (MQ=255)                                                           tACCACGGCGGGTGCGAACGGCAACTTTGCCTGATTCCACCTCTTTATCACCACAGACCAGCATATATGGGACGCGACGAAAAGTGTGCTCGCGGATTTTAAAGCCAATCTTCTCATTTCTCAAGTCTGCTTTAACACGa                                                                             <  1:1073984/140‑1 (MQ=255)                                                           tACCACGGCGGGTGCGAAAGGCCACTTTGCCTGATTCCCCCTCTTTATCACCACAGACCAGCATATATGGGACGCGACGAAAAGAGTGCTCGCGGATTTTAAAGCCCATCTTCTCATTTCTCAAGTCTGCTTTAACACGa                                                                             >  2:346219/1‑140 (MQ=255)                                                               aCGGCGGGTGCGAACGGCAACTTTGCCTGATTCCACCTCTTTATCACCACAGACCAGCATATATGGGACGCGACGAAAAGTGTGCTCGCGGATTTTAAAGCCAATCTTCTCATTTCTCAAGTCTGCTTTAACACGAATGc                                                                         >  2:559146/1‑140 (MQ=255)                                                               aCGGCGGGTGCGAACGGCAACTTTGCCTGATTCCACCTCTTTATCACCACAGACCAGCATATATGGGACGCGACGAAAAGTGTGCTCGCGGATTTTAAAGCCAATCTTCTCATTTCTCAAGTCTGCTTTAACACGAATGc                                                                         <  1:609289/140‑1 (MQ=255)                                                                cggcggGTGCGAACGGCAACTTTGCCTGATTCCACCTCTTTATCACCACAGACCAGCATATATGGGACGCGACGAAAAGTGTGCTCGCGGATTTTAAAGCCAATCTTCTCATTTCTCAAGTCTGCTTTAACACGAATGcc                                                                        <  2:545501/140‑1 (MQ=255)                                                                cggcggGTGCGAACGGCAACTTTGCCTGATTCCACCTCTTTATCACCACAGACCAGCATATATGGGAAGCGACGAAAAGGGTGCTCGCGGATTTTAAAGCCAATCTTCTCACTTTTCAAGTGTGCTTTAAAACGAAAGcc                                                                        >  2:315983/1‑140 (MQ=255)                                                                 ggcggGTGCGAACGGCAACTTTGCCTGATTCCACCTCTTTATCACCACAGACCAGCATATATGGGACGCGACGAAAAGTGTGCTCGCGGATTTTAAAGCCAATCTTCTCATTTCTCAAGTCTGCTTTAACACGAATGccc                                                                       <  1:436324/140‑1 (MQ=255)                                                                  gcggGTGCGAACGGCAACTTTGCCTGATTCCACCTCTTTATCACCACAGACCAGCATATATGGGACGCGACGAAAAGTGTGCTCGCGGATTTTAAAGCCAATCTTCTCATTTCTCAAGTCTGCTTTAACACGAATGCCCg                                                                      <  1:522044/140‑1 (MQ=255)                                                                     ggTGCGAACGGCAACTTTGCCTGATTCCACCTCTTTATCACCACAGACCAGCATATATGGGACGCGACGAAAAGTGTGCTCGCGGATTTTAAAGCCAATCTTCTCATTTCTCAAGTCTGCTTTAACACGAATGCCCGCAt                                                                   <  1:109536/140‑1 (MQ=255)                                                                         cGAACGGCAACTTTGCCTGATTCCACCTCTTTATCACCACAGACCAGCATATATGGGACGCGACGAAAAGTGTGCTCGCGGATTTTAAAGCCAATCTTCTCATTTCTCAAGTCTGCTTTAACACGAATGCCCGCATTTGa                                                               <  2:1045514/140‑1 (MQ=255)                                                                                cAACTTTGCCTGATTCCACCTCTTTATCACCACAGACCAGCATATATGGGACGCGACGAAAAGTGTGCTCGCGGATTTTAAAGCCAATCTTCTCATTTCTCAAGTCTGCTTTAACACGAATGCCCGCATTTGATAGtttt                                                        >  2:906465/1‑140 (MQ=255)                                                                                cAACTTTGCCTGATTCCACCTCTTTATCACCACAGACCAGCATATATGGGACGCGACGAAAAGTGTGCTCGCGGATTTTAAAGCCAATCTTCTCATTTCTCAAGTCTGCTTTAACACGAATGCCCGCATTTGATAGtttt                                                        >  2:1151122/1‑140 (MQ=255)                                                                                       gCCTGATTCCACCTCTTTATCACCACAGACCAGCATATATGGGACGCGACGAAAAGTGTGCTCGCGGATTTTAAAGGCAATCTTCTCATTTCTCAAGTCTGCTTTAACACGAATGCCCGCATTTGATAGTTTTTGCGTCa                                                 <  2:417048/140‑1 (MQ=255)                                                                                            aTTCCACCTCTTTATCACCACAGACCAGCATATATGGGACGCGACGAAAAGTGTGCTCGCGGATTTTAAAGCCAATCTTCTCATTTCTCAAGTCTGCTTTAACACGAATGCCCGCATTTGATAGTTTTTGCGTCAATTCg                                            <  2:563666/140‑1 (MQ=255)                                                                                              tCCACCTGTTTATCACCACAGACCAGCATATATGGGACGCGACGAAAAGTGTGCTCGCGGATTTTAAAGCCAATCTTCTCATTTCTCAAGTCTGCTTTAACACGAATGCCCGCATTTGATAGTTTTTGCGTCAATTCGtt                                          <  1:274022/140‑1 (MQ=255)                                                                                              tCCACCTCTTTATCACCACAGACCAGCATATATGGGACGCGACGAAAAGTGTGCTCGCGGATTTTAAAGCCAATCTTCTCATTTCTCAAGTCTGCTTTAACACGAATGCCCGCATTTGATAGTTTTTGCGTCAATTCGtt                                          <  1:1314183/140‑1 (MQ=255)                                                                                              tCCACCTCTTTATCACCACAGACCAGCATATATGGGACGCGACGAAAAGTGTGCTCGCGGATTTTAAAGCCAATCTTCTCATTTCTCAAGTCTGCTTTAACACGAATGCCCGCATTTGATAGTTTTTGCGTCAATTCGtt                                          >  2:1314183/1‑140 (MQ=255)                                                                                              tCCACCTCTTTATCACCACAGACCAGCATATATGGGACGCGACGAAAAGTGTGCTCGCGGATTTTAAAGCCAATCTTCTCATTTCTCAAGTCTGCTTTAACACGAATGCCCGCATTTGATAGTTTTTGCGTCAATTCGtt                                          <  1:1277008/140‑1 (MQ=255)                                                                                              tCCACCTCTTTATCACCACAGACCAGCATATATGGGACGCGACGAAAAGTGTGCTCGCGGATTTTAAAGCCAATCTTCTCATTTCTCAAGTCTGCTTTAACACGAATGCCCGCATTTGATAGTTTTTGCGTCAATTCGtt                                          <  2:790737/140‑1 (MQ=255)                                                                                                cACCTCTTTATCACCACAGACCAGCATATATGGGACGCGACGAAAAGTGTGCTCGCGGATTTTAAAGCCAATCTTCTCATTTCTCAAGTCTGCTTTAACACGAATGCCCGCATTTGATAGTTTTTGCGTCAATTCGTTaa                                        <  2:528135/140‑1 (MQ=255)                                                                                                      tttATCACCACAGACCAGCATATATGGGACGCGACGAAAAGTGTGCTCGCGGATTTTAAAGCCAATCTTCTCATTTCTCAAGTCTGCTTTAACACGAATGCCCGCATTTGATAGTTTTTGCGTCAATTCGTTAACGTAtt                                  <  1:1263500/140‑1 (MQ=255)                                                                                                      tttATCACCACAGACCAGCATATATGGGACGCGACGAAAAGTGTGCTCGCGGATTTTAAAGCCAATCTTCTCATTTCTCAAGTCTGCTTTAACACGAATGCCCGCATTTGATAGTTTTTGCGTCAATTCGTTAACGTAtt                                  <  1:1151122/140‑1 (MQ=255)                                                                                                       ttATCACCACAGACCAGCATATATGGGACGCGACGAAAAGTGTGCTCGCGGATTTTAAAGCCAATCTTCTCATTTCTCAAGTCTGCTTTAACACGAATGCCCGCATTTGATAGTTTTTGCGTCAATTCGTTAACGTAtt                                  <  1:1222281/139‑1 (MQ=255)                                                                                                       ttATCACCACAGACCAGCATATATGGGACGCGACGAAAAGTGTGCTCGCGGATTTTAAAGCCAATCTTCTCATTTCTCAAGTCTGCTTTAACACGAATGCCCGCATTTGATAGTTTTTGCGTCAATTCGTTAACGTAtt                                  >  2:1222281/1‑139 (MQ=255)                                                                                                       ttATCACCACAGACCAGCATATATGGGACGCGACGAAAAGTGTGCTCGCGGATTTTAAAGCCAATCTTCTCATTTCTCAAGTCTGCTTTAACACGAATGCCCGCATTTGATAGTTTTTGCGTCAATTCGTTAACGTATTc                                 >  2:494092/1‑140 (MQ=255)                                                                                                        tATCACCACAGACCAGCATATATGGGACGCGACGAAAAGTGTGCTCGCGGATTTTAAAGCCAATCTTCTCATTTCTCAAGTCTGCTTTAACACGAATGCCCGCATTTGATAGTTTTTGCGTCAATTCGTTAACGTATTCa                                >  2:231029/1‑140 (MQ=255)                                                                                                        tATCACCACAGACCAGCATATATGGGACGCGACGAAAAGTGTGCTCGCGGATTTTAAAGCCAATCTTCTCATTTCTCAAGTCTGCTTTAACACGAATGCCCGCATTTGATAGTTTTTGCGTCAATTCGTTAACGTATTCa                                >  1:948751/1‑140 (MQ=255)                                                                                                         aTCACCACAGACCAGCATATATGGGACGCGACGCAAAGTATGCTCGCGGATTTTAAAGCCAATCTTCTCATTTCTCAAGTCTGCTTTAACACGAATGCCCGCATTTGATAGTTTTTGCGTCAATTCGTTAACGTATTCAg                               >  2:600960/1‑140 (MQ=255)                                                                                                            accacAGACCAGCATATATGGGACGCGACGAAAAGTGTGCTCGCGGATTTTAAAGCCAATCTTCTCATTTCTCAAGTCTGCTTTAACACGAATGCCCGCATTTGATAGTTTTTGCGTCAATTCGTTAACGTATTCAGACt                            >  1:753612/1‑140 (MQ=255)                                                                                                             ccacAGACCAGCATATATGGGACGCGACGAAAAGTCTGCTCGCGGATTTTAAAGTCAATCTTCTCATTTCTCAAGTCTGCTTTAACACGAATGCCCGCATTTGATAGTTTTTGCGTCAATTCGATAACGTATTCAGACtg                           <  2:831099/140‑1 (MQ=255)                                                                                                              cacaGACCAGCATATATGGGACGCGACGAAAAGTGTGCTCGCGGATTTTAAAGCCAATCTTCTCATTTCTCAAGTCTGCTTTAACACGAATGCCCGCATTTGATAGTTTTTGCGTCAATTCGTTAACGTATTCAGACtgt                          >  1:288953/1‑140 (MQ=255)                                                                                                               acaGACCAGCATATATGGGACGCGACGAAAAGTGTGCTCGCGGATTTTAAAGCCAATCTTCTCATTTCTCAAGTCTGCTTTAACACGAATGCCCGCATTTGATAGTTTTTGCGTCAATTCGTTAACGTATTCAGACtgtg                         <  2:571625/140‑1 (MQ=255)                                                                                                                caGACCAGCATATATGGGACGCGACGAAAAGTGTGCTCGCGGATTTTAAAGCCAATCTTCTCATTTCTCAAGTCTGCTTTAACACGAATGCCCGCATTTGATAGTTTTTGCGTCAATTCGTTAACGTATTCAGACTGTGa                        >  2:1048838/1‑140 (MQ=255)                                                                                                                caGACCAGCATATATGGGACGCGACGAAAAGTGTGCTCGCGGATTTTAAAGCCAATCTTCTCATTTCTCAAGTCTGCTTTAACACGAATGCCCGCATTTGATAGTTTTTGCGTCAATTCGTTAACGTATTCAGACTGTGa                        >  1:396512/1‑140 (MQ=255)                                                                                                                caGACCAGCATATATGGGACGCGACGAAAAGTGTGCTCGCGGATTTTAAAGCCAATCTTCTCATTTCTCAAGTCTGCTTTAACACGAATGCCCGCATTTGATAGTTTTTGCGTCAATTCGTTAACGTATTCAGACTGTGa                        >  1:404997/1‑140 (MQ=255)                                                                                                                  gACCAGCATATATGGGACGCGACGAAAAGTGTGCTCGCGGATTTTAAAGCCAATCTTCTCATTTCTCAAGTCTGCTTTAACACGAATGCCCGCATTTGATAGTTTTTGCGTCAATTCGTTAACGTATTCAGACTGTGAAt                      <  1:1237311/140‑1 (MQ=255)                                                                                                                  gACCAGCATATATGGGACGCGACGAAAAGTGTGCTCGCGGATTTTAAAGCCAATCTTCTCATTTCTCAAGTCTGCTTTAACACGAATGCCCGCATTTGATAGTTTTTGCGTCAATTCGTTAACGTATTCAGACTGTGAAt                      <  2:396512/140‑1 (MQ=255)                                                                                                                  gACCAGCATATATGGGACGCGACGAAAAGTGTGCTCGCGGATTTTAAAGCCAATCTTCTCATTTCTCAAGTCTGCTTTAACACGAATGCCCGCATTTGATAGTTTTTGCGTCAATTCGTTAACGTATTCAGACTGTGAAt                      <  1:174253/140‑1 (MQ=255)                                                                                                                   aCCAGCATATATGGGACGCGACGAAAAGTGTGCTCGCGGATTTTAAAGCCAATCTTCTCATTTCTCAAGTCTGCTTTAACACGAATGCCCGCATTTGATAGTTTTTGCGTCAATTCGTTAACGTATTCAGACTGTGAATc                     >  2:107575/1‑140 (MQ=255)                                                                                                                   aCCAGCATATATGGGACGCGACGAAAAGTGTGCTCGCGGATTTTAAAGCCAATCTTCTCATTTCTCAAGTCTGCTTTAACACGAATGCCCGCATTTGATAGTTTTTGCGTCAATTCGTTAACGTATTCAGACTGTGAATc                     >  2:443597/1‑140 (MQ=255)                                                                                                                   aCCAGCATATATGGGACGCGACGAAAAGTGTGCTCGCGGATTTTAAAGCCAATCTTCTCATTTCTCAAGTCTGCTTTAACACGAATGCCCGCATTTGATAGTTTTTGCGTCAATTCGTTAACGTATTCAGACTGTGAATc                     >  1:227715/1‑140 (MQ=255)                                                                                                                       gCATATATGGGACGCGACGAAAAGTGTGCTCGCGGATTTTAAAGCCAATCTTCTCATTTCTCAAGTCTGCTTTAACACGAATGCCCGCATTTGATAGTTTTTGCGTCAATTCGTTAACGTATTCAGACTGTGAATCGGTa                 <  2:125514/140‑1 (MQ=255)                                                                                                                       gCATATATGGGACGCGACGAAAAGTGTGCTCGCGGATTTTAAAGCCAATCTTCTCATTTCTCAAGTCTGCTTTAACACGAATGCCCGCATTTGATAGTTTTTGCGTCAATTCGTTAACGTATTCAGACTGTGAATCGGTa                 <  1:603789/140‑1 (MQ=255)                                                                                                                       gCATATATGGGACGCGACGAAAAGTGTGCTCGCGGATTTTAAAGCCAATCTTCTCATTTCTCAAGTCTGCTTTAACACGAATGCCCGCATTTGATAGTTTTTGCGTCAATTCGTTAACGTATTCAGACTGTGAATCGGTa                 <  1:579925/140‑1 (MQ=255)                                                                                                                       gCATATATCGGACGCGACGAAAAGTGTGCTCGCGGATTTTAAAGCCAATCTTCTCATTTCTCAAGTCTGCTTTAACACGAATGCCCGCATTTGATAGTTTTTGCGTCAATTCGTTAACGTATTCAGACTGTGAATCGGTa                 <  2:903152/140‑1 (MQ=255)                                                                                                                        cATATATGGGACGCGACGAAAAGTGTGCTCGCGGATTTTAAAGCCAATCTTCTCATTTCTCAAGTCTGCTTTAACACGAATGCCCGCATTTGATAGTTTTTGCGTCAATTCGTTAACGTATTCAGACTGTGAATCGGTaa                >  2:863874/1‑140 (MQ=255)                                                                                                                        cATATATGGGACGCGACGAAAAGTGTGCTCGCGGATTTTAAAGCCAATCTTCTCATTTCTCAAGTCTGCTTTAACACGAATGCCCGCATTTGATAGTTTTTGCGTCAATTCGTTAACGTATTCAGACTGTGAATCGGTaa                >  1:514956/1‑140 (MQ=255)                                                                                                                        cATATATGGGACGCGACGAAAAGTGTGCTCGCGGATTTTAAAGCCAATCTTCTCATTTCTCAAGTCTGCTTTAACACGAATGCCCGCATTTGATAGTTTTTGCGTCAATTCGTTAACGTATTCAGACTGTGAATCGGTaa                >  2:283414/1‑140 (MQ=255)                                                                                                                        cATATATGGGACGCGACGAAAAGTGTGCTCGCGGATTTTAAAGCCAATCTTCTCATTTCTCAAGTCTGCTTTAACACGAATGCCCGCATTTGATAGTTTTTGCGTCAATTCGTTAACGTATTCAGACTGTGAATCGGTaa                >  2:1130098/1‑140 (MQ=255)                                                                                                                          tatatGGGACGCGACGAAAAGTGTGCTCGCGGATTTTAAAGCCAATCTTCTCATTTCTCAAGTCTGCTTTAACACGAATGCCCGCATTTGATAGTTTTTGCGTCAATTCGTTAACGTATTCAGACTGTGAATCGGTAata              <  2:276049/140‑1 (MQ=255)                                                                                                                                  acgcgacgaAAAGTGTGCTCGCGGATTTTAAAGCCAATCTTCTCATTTCTCAAGTCTGCTTTAACACGAATGCCCGCATTTGATAGTTTTTGCGTCAATTCGTTAACGTATTCAGACTGTGAATCGGTAATATTCATGAt      >  2:1062515/1‑140 (MQ=255)                                                                                                                                  acgcgacgaAAAGTGTGCTCGCGGATTTTAAAGCCAATCTTCTCATTTCTCAAGTCTGCTTTAACACGAATGCCCGCATTTGATAGTTTTTGCGTCAATTCGTTAACGTATTCAGACTGTGAATCGGTAATATTCATGAt      >  1:1196056/1‑140 (MQ=255)                                                                                                                                  acgcgacgaAAAGTGTGCTCGCGGATTTTAAAGCCAATCTTCTCATTTCTCAAGTCTGCTTTAACACGAATGCCCGCATTTGATAGTTTTTGCGTCAATTCGTTAACGTATTCAGACTGTGAATCGGTAATATTCATGAt      >  1:659289/1‑140 (MQ=255)                                                                                                                                  acgcgacgaAAAGTGTGCTCGCGGATTTTAAAGCCAATCTTCTCATTTCTCAAGTCTGCTTTAACACGAATGCCCGCATTTGATAGTTTTTGCGTCAATTCGTTAACGTATTCAGACTGTGAATCGGTAATATTCATGAt      >  1:385174/1‑140 (MQ=255)                                                                                                                                   cgcgacgaAAAGTGTGCTCGCGGATTTTAAAGCCAATCTTCTCATTTCTCAAGTCTGCTTTAACACGAATGCCCTCATTTGATAGTTTTTGCGTCAATTCGTTAACGTATTCAGACTGTGAATCGGTAATATTCATGATa     <  2:633060/140‑1 (MQ=255)                                                                                                                                   cgcgacgaAAAGTGTGCTCGCGGATTTTAAAGCCAATCTTCTCATTTCTCAAGTCTGCTTTAACACGAATGCCCGCATTTGATAGTTTTTGCGTCAATTCGTTAACGTATTCAGACTGTGAATCGGTAATATTCATGATa     >  1:131834/1‑140 (MQ=255)                                                                                                                                   cgcgacgaAAAGTGTGCTCGCGGATTTTAAAGCCAATCTTCTCATTTCTCAAGTCTGCTTTAACACGAATGCCCGCATTTGATAGTTTTTGCGTCAATTCGTTAACGTATTCAGACTGTGAATCGGTAATATTCATGATa     <  2:965200/140‑1 (MQ=255)                                                                                                                                    gcgacgaAAAGTGTGCTCGCGGATTTTAAAGCCAATCTTCTCATTTCTCAAGTCTGCTTTAACACGAATGCCCGCATTTGATAGTTTTTGCGTCAATTCGTTAACGTATTCAGACTGTGAATCGGTAATATTCATGATaa    <  1:863874/140‑1 (MQ=255)                                                                                                                                    gcgacgaAAAGTATGCTCGCGGATTTTAAAGCCAATCTTCTCATTTCTCAAGTCTGCTTTAACACGAATGCCCGCATTTGATAGTTTTTGCGTCAATTCGTTAACGTATTCAGACTGTGAATCGGTAATATTCATGATaa    <  1:600960/140‑1 (MQ=255)                                                                                                                                     cgacgaAAAGTGTGCTCGCGGATTTTAAAGCCAATCTTCTCATTTCTCAAGTCTGCTTTAACACGAATGCCCGCATTTGATAGTTTTTGCGTCAATTCGTTAACGTATTCAGACTGTGAATCGGTAATATTCATGATaac   >  1:705219/1‑140 (MQ=255)                                                                                                                                     cgacgaAAAGTGTGCTCGCGGATTTTAAAGCCAATCTTCTCATTTCTCAAGTCTGCTTTAACACGAATGCCCGCATTTGATAGTTTTTGCGTCAATTCGTTAACGTATTCAGACTGTGAATCGGTAATATTCATGATaac   >  1:619495/1‑140 (MQ=255)                                                                                                                                      gacgaAAAGTGTGCTCGCGGATTTTAAAGCCAATCTTCTCATTTCTCAAGTCTGCTTTAACACGAATGCCCGCATTTGATAGTTTTTGCGTCAATTCGTTAACGTATTCAGACTGTGAATCGGTAATATTCATGATaaca  >  2:516529/1‑140 (MQ=255)                                                                                                                                          |                                                                                                                                        CGAATCTCTTGTTGCAGCTTCTCGATCACTTCATTTACGTCCATGCTTCCCAGGTCTTTACCACGGCGGGTGCGAACGGCAACTTTGCCTGATTCCACCTCTTTATCACCACAGACCAGCATATATGGGACGCGACGCAAAGTGTGCTCGCGGATTTTAAAGCCAATCTTCTCATTTCTCAAGTCTGCTTTAACACGAATGCCCGCATTTGATAGTTTTTGCGTCAATTCGTTAACGTATTCAGACTGTGAATCGGTAATATTCATGATAACA  >  NC_000913/1800673‑1800945

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑