Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,029,333	G→T	L12I (CTC→ATC)	yedI ←	conserved inner membrane protein YedI

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,029,333	0	G	T	100.0%	243.5 / NA	68	L12I (CTC→ATC)	yedI	conserved inner membrane protein YedI
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (16/52);  total (16/52)

AGTTCCCGGTTGGCCCGCACGCCTGAAACTTGTTGCGCATTGAGCGATAAGTCATCCCCTAATACACCGGCGGTTTTCTTCGCCGCCAGTTTGCCCATCACGGAGATATCGTCCAGTAGTGTGGCGATATCATCGAGCAACGTCAGTAAACTACTCCCGGCCAAAAGCATTCCCTCTCATTTTTATTGTTGAAGGGTGTAAGTATGAAGCAAAAACGGCAGCTCTGATACTTGCTTCACAGGTCAACATTTTTTTAACAATCAAGTGCA  >  NC_000913/2029197‑2029465                                                                                                                                         |                                                                                                                                     aGTTCCCGGTTGGCCCGCTCGCCTGAAACTTGTTGCGCATTGAGCGATAAGTCATCCCCTAATACACCGGCGGTTTTCTTCGCCGCCAGTTTGCCCATCACGGAGATATCGTCCAGTAGTGTGGCGATATCATCGATCaa                                                                                                                                   <  2:833064/140‑1 (MQ=255) aGTTCCCGGTTGGCCCGCACGCCTGAAACTTGTTGCGCATTGAGCGATAAGTCATCCCCTAATACACCGGCGGTTTTCTTCGCCGCCAGTTTGCCCATCACGGAGATATCGTCCAGTAGTGTGGCGATATCATCGATCaa                                                                                                                                   <  2:1054885/140‑1 (MQ=255)  gTTCCCGGTTGGCCCGCACGCCTGAAACTTGTTGCGCATTGAGCGATAAGTCATCCCCTAATACACCGGCGGTTTTTTTCGCCCCCAGTTTGCCCATCACGGAGATATCGTCCAGTAGTGTGGCGATATCATCGATCAAc                                                                                                                                  >  2:70291/1‑140 (MQ=255)  gTTCCCGGTTGGCCCGCACGCCTGAAACTTGTTGCGCATTGAGCGATAAGTCATCCCCTAATACACCGGCGGTTTTCTTCGCCGCCAGTTTGCCCATCACGGAGATATCGTCCAGTAGTGTGGCGATATCATCGATCAAc                                                                                                                                  <  1:593300/140‑1 (MQ=255)    tCCCGGTTGGCCCGCACGCCTGAAACTTGTTGCGCATTGAGCGATAAGTCATCCCCTAATACACCGGCGGTTTTTTTTGCCGCCAGTTTGCCCACCCCGGAGATATCGTCCCGTAGTGTGGCGATATCATCGATCCACgc                                                                                                                                >  2:613455/1‑139 (MQ=255)    tCCCGGTTGGCCCGCACGCCTGAAACTTGTTGCGCATTGAGCGATAAGTCATCCCCTAATACACCGGCGGTTTTCTTCGCCGCCAGTTTGCCCATCACGGAGATATCGTCCAGTAGTGTGGCGATATCATCGATCAACGt                                                                                                                                <  1:1288423/140‑1 (MQ=255)        ggTTGGCCCGCACGCCTGAAACTTGTTGCGCATTGAGCGATAAGTCATCCCCTAATACACCGGCGGTTTTCTTCGCCGCCAGTTTGCCCATCACGGAGATATCGTCCAGTAGTGTGGCGATATCATCGATCAACGTCAGt                                                                                                                            <  1:588700/140‑1 (MQ=255)         gTTGGCCCGCACGCCTGAAACTTGTTGCGCATTGAGCGATAAGTCATCCCCTAATACACCGGCGGTTTTCTTCGCCGCCAGTTTGCCCATCACGGAGATATCGTCCAGTAGTGTGGCGATATCATCGATCAACGTCAGTa                                                                                                                           >  2:825100/1‑140 (MQ=255)              cccGCACGCCTGAAACTTGTTGCGCATTGAGCGATAAGTCATCCCCTAATACACCGGCGGTTTTCTTCGCCGCCACTTTTCCCCTCCCCGAGATATCGTCCAGTTGGGTGGCGATATCATCGATCAACGTCAGTAAacta                                                                                                                      >  2:723520/1‑140 (MQ=255)                 gCACGCCTGAAACTTGTTGCGCATTGAGCGATAAGTCATCCCCTAATACACCGGCGGTTTTCTTCGCCGCCAGTTTGCCCATCACGGAGATATCGTCCAGTAGTGTGGCGATATCATCGATCAACGTCAGTAAACTACTc                                                                                                                   <  2:1173147/140‑1 (MQ=255)                 gCACGCCTGAAACTTGTTGCGCATTGAGCGATAAGTCATCCCCTAATACACCGGCGGTTTTCTTCGCCGCCAGTTTGCCCATCACGGAGATATCGTCCAGTAGTGTGGCGATATCATCGATCAACGTCAGTAAACTACTc                                                                                                                   <  2:905420/140‑1 (MQ=255)                       cTGAAACTTGTTGCGCATTGAGCGCTAAGTCATCCCCTAATACACCGGCGGTTTTCTTCGCCGCCAGTTTGCCCATCACGGAGATATCGTCCAGTAGTGTGGCGATATCATCGATCAACGTCAGTAAACTACTCCCGGcc                                                                                                             <  2:298243/140‑1 (MQ=255)                       cTGAAACTTGTTGCGCATTGAGCGATAAGTCATCCCCTAATACACCGGCGGTTTTCTTCGCCGCCAGTTTGCCCATCACGGAGATATCGTCCAGTAGTGTGGCGATATCATCGATCAACGTCAGTAAACTACTCCCGGcc                                                                                                             >  2:390061/1‑140 (MQ=255)                          aaaCTTGTTGCGCATTGAGCGATAAGTCATCCCCTAATACACCGGCGGTTTTCTTCGCCGCCAGTTTGCCCATCACGGAGATATCGTCCAGTAGTGTGGCGATATCATCGATCAACGTCAGTAAACTACTCCCGGCCaaa                                                                                                          <  1:557651/140‑1 (MQ=255)                                      cATTGAGCGATAAGTCATCCCCTAATACACCGGCGGTTTTCTTCGCCGCCAGTTTGCCCATCACGGAGATATCGTCCAGTAGTGTGGCGATATCATCGATCAACGTCAGTAAACTACTCCCGGCCAAAAGCATTCCctct                                                                                              <  1:760523/140‑1 (MQ=255)                                       aTTGAGCGATAAGTCATCCCCTAATACACCGGCGGTTTTCTTCGCCGCCAGTTTGTCCATCACGGAGATATCGTCCAGTAGTGTGGCGATATCATCGATCAACGTCAGTAAACTACTCCCGGCCAAAAGCATTCCCtctc                                                                                             <  2:606888/140‑1 (MQ=255)                                            gCGATAAGTCATCCCCTAATACACCGGCGGTTTTCTTCGCCGCCAGTTTGCCCATCACGGAGATATCGTCCAGTAGTGTGGCGATATCATCGATCAACGTCAGTAAACTACTCCCGGCCAAAAGCATTCCCTCTCAtttt                                                                                        <  2:1273956/140‑1 (MQ=255)                                              gATAAGTCATCCCCTAATACACCGGCGGTTTTCTTCGCCGCCAGTTTGCCCATCACGGAGATATCGTCCAGTAGTGTGGCGATATCATCGATCAACGTCAGTAAACTACTCCCGGCCAAAAGCATTCCCTCTCATTTTTa                                                                                      <  2:63142/140‑1 (MQ=255)                                              gATAAGTCATCCCCTAATACACCGGCGGTTTTCTTCGCCGCCAGTTTGCCCATCACGGAGATATCGTCCAGTAGTGTGGCGATATCATCGATCAACGTCAGTAAACTACTCCCGGCCAAAAGCATTCCCTCTCATTTTTa                                                                                      <  1:678914/140‑1 (MQ=255)                                              gATAAGTCATCCCCTAATACACCGGCGGTTTTCTTCGCCGCCAGTTTGCCCATCACGGAGATATCGTCCAGTAGTGTGGCGATATCATCGATCAACGTCAGTAAACTACTCCCGGCCAAAAGCATTCCCTCTCATTTTTa                                                                                      <  2:766390/140‑1 (MQ=255)                                                    tCATCCCCTAATACACCGGCGGTTTTCTTCGCCGCCAGTTTGCCCATCACGGAGATATCGTCCAGTAGTGTGGCGATATCATCGATCAACGTCAGTAAACTACTCCCGGCCAAAAGCATTCCCTCTCATTTTTAttgttg                                                                                <  1:390061/140‑1 (MQ=255)                                                    tCATCCCCTAATACACCGGCGGTTTTCTTCGCCGCCAGTTTGCCCATCACGGAGATATCGTCCAGTAGTGTGGCGATATCATCGATCAACGTCAGTAAACTACTCCCGGCCAAAAGCATTCCCTCTCATTTTTAttgttg                                                                                <  2:1152738/140‑1 (MQ=255)                                                    tCATCCCCTAATACACCGGCGGTTTTCTTCGCCGCCAGTTTGCCCATCACGGAGATATCGTCCAGTAGTGTGGCGATATCATCGATCAACGTCAGTAAACTACTCCCGGCCAAAAGCATTCCCTCTCATTTTTAttgttg                                                                                <  2:913281/140‑1 (MQ=255)                                                      aTCCCCTAATACACCGGCGGTTTTCTTCGCCGCCAGTTTGCCCATCACGGAGATATCGTCCAGTAGTGTGGCGATATCATCGATCAACGTCAGTAAACTACTCCCGGCCAAAAGCATTCCCTCTCATTTTTATTGTTGaa                                                                              <  1:1110524/140‑1 (MQ=255)                                                      aTCCCCTAATACACCGGCGGTTTTCTTCGCCGCCAGTTTGCCCATCACGGAGATATCGTCCAGTAGTGTGGCGATATCATCGATCAACGTCAGTAAACTACTCCCGGCCAAAAGCATTCCCTCTCATTTTTATTGTTGaa                                                                              <  2:1054425/140‑1 (MQ=255)                                                      aTCCCCTAATACACCGGCGGTTTTCTTCGCCGCCAGTTTGCCCATCACGGAGATATCGTCCAGTAGTGTGGCGATATCATCGATCAACGTCAGTAAACTACTCCCGGCCAAAAGCATTCCCTCTCATTTTTATTGTTGaa                                                                              <  2:173865/140‑1 (MQ=255)                                                      aTCCCCTAATACACCGGCGGTTTTCTTCGCCGCCAGTTTGCCCATCACGGAGATATCGTCCAGTAGTGTGGCGATATCATCGATCAACGTCAGTAAACTACTCCCGGCCAAAAGCATTCCCTCTCATTTTTATTGTTGaa                                                                              <  2:260420/140‑1 (MQ=255)                                                      aTCCCCTAATACACCGGCGGTTTTCTTCGCCGCCAGTTTGCCCATCACGGAGATATCGTCCAGTAGTGTGGCGATATCATCGATCAACGTCAGTAAACTACTCCCGGCCAAAAGCATTCCCTCTCATTTTTATTGTTGaa                                                                              <  2:966135/140‑1 (MQ=255)                                                      aTCCCCTAATACACCGGCGGTTTTCTTCGCCGCCAGTTTGCCCATCACGGAGATATCGTCCAGTAGTGTGGCGATATCATCGATCAACGTCAGTAAACTACTCCCGGCCAAAAGCATTCCCTCTCATTTTTATTGTTGaa                                                                              <  2:968996/140‑1 (MQ=255)                                                      aTCCCCTAATACACCGGCGGTTTTCTTCGCCGCCAGTTTGCCCATCACGGAGATATCGTCCAGTAGTGTGGCGATATCATCGATCAACGTCAGTAAACTACTCCCGGCCAAAAGCATTCCCTCTCATTTTTATTGTTGaa                                                                              <  1:310659/140‑1 (MQ=255)                                                      aTCCCCTAATACACCGGCGGTTTTCTTCGCCGCCAGTTTGCCCATCACGGAGATATCGTCCAGTAGTGTGGCGATATCATCGATCAACGTCAGTAAACTACTCCCGGCCAAAAGCATTCCCTCTCATTTTTATTGTTGaa                                                                              <  1:783391/140‑1 (MQ=255)                                                      aTCCCCTAATACACCGGCGGTTTTCTTCGCCGCCAGTTTGCCCATCACGGAGATATCGTCCAGTAGTGTGGCGATATCATCGATCAACGTCAGTAAACTACTCCCGGCCAAAAGCATTCCCTCTCATTTTTATTGTTGaa                                                                              <  1:1261978/140‑1 (MQ=255)                                                      aTCCCCTAATACACCGGCGGTTTTCTTCGCCGCCAGTTTGCCCATCACGGAGATATCGTCCAGTAGTGTGGCGATATCATCGATCAACGTCAGTAAACTACTCCCGGCCAAAAGCATTCCCTCTCATTTTTATTGTTGaa                                                                              <  1:825100/140‑1 (MQ=255)                                                      aTCCCCTAATACACCGGCGGTTTTCTTCGCCGCCAGTTTGCCCATCACGGAGATATCGTCCAGTAGTGTGGCGATATCATCGATCAACGTCAGTAAACTACTCCCGGCCAAAAGCATTCCCTCTCATTTTTATTGTTGaa                                                                              <  1:813931/140‑1 (MQ=255)                                                      aTCCCCTAATACACCGGCGGTTTTCTTCCCCCCCAGTTTGCCCATCACGGAGATATCGTCCAGGAGGGTGGCGATATCATCGATCAACGTCAGTAAACTACTCCCGGCCCAAAGCATTTCCTCTCCTTTTTTTTTTTGaa                                                                              >  2:741940/1‑140 (MQ=255)                                                       tCCCCTAATACACCGGCGGTTTTCTTCGCCGCCAGTTTGCCCATCACGGAGATATCGTCCAGTAGTGTGGCGATATCATCGATCAACGTCAGTAAACTACTCCCGGCCAAAAGCATTCCCTCTCATTTTTATTGTTGAAg                                                                             <  1:1134109/140‑1 (MQ=255)                                                       tCCCCTAATACACCGGCGGTTTTCTTCGCCGCCAGTTTGCCCATCACGGAGATATCGTCCAGTAGTGTGGCGATATCATCGATCAACGTCAGTAAACTACTCCCGGCCAAAAGCATTCCCTCTCATTTTTATTGTTGAAg                                                                             >  2:754260/1‑140 (MQ=255)                                                                  acCGGCGGTTTTCTTCGCCGCCAGTTTGCCCATCCCGGAGATATCGTCCAGTAGTGTGGCGATATCATCGATCAACGTCAGTAAACTACTCCCGGCCAAAAGCATTCCCTCTCATTTTTATTGTTGAAGGGTGTAAGTAt                                                                  <  2:436875/140‑1 (MQ=255)                                                                  acCGGCGGTTTTCTTCGCCGCCAGTTTGCCCATCACGGAGATATCGTCCAGTAGTGGGGCGATATCATCGATCAACGTCAGTAAACTACTCCCGGCCAAAAGCATTCCCTCTCATTTTTATTGTTGAAGGGTGTAAGTAt                                                                  >  2:415851/1‑140 (MQ=255)                                                                   ccGGCGGTTTTCTTCGCCGCCAGTTTGCCCATCACGGAGATATCGTCCAGTAGTGTGGCGATATCATCGATCAACGTCAGTAAACTACTCCCGGCCAAAAGCATTCCCTCTCATTTTTATTGTTGAAGGGTGTAAGTATg                                                                 <  1:766960/140‑1 (MQ=255)                                                                    cggcggTTTTCTTCGCCGCCAGTTTGCCCATCACGGAGATATCGTCCAGTAGTGTGGCGATATCATCGATCAACGTCAGTAAACTACTCCCGGCCAAAAGCATTCCCTCTCATTTTTATTGTTGAAGGGTGTAAGTATGa                                                                >  2:1088196/1‑140 (MQ=255)                                                                          ttttCTTCGCCGCCAGTTTGCCCATCACGGAGATATCGTCCAGTAGTGTGGCGATATCATCGATCAACGTCAGTAAACTACTCCCGGCCAAAAGCATTCCCTCTCATTTTTATTGTTGAAGGGTGTAAGTATGAAGCaaa                                                          <  2:1067627/140‑1 (MQ=255)                                                                                tcGCCGCCAGTTTGCCCATCACGGAGATATCGTCCAGTAGTGTGGCGATATTATCGATCAACGTCAGTAAACTACTCCCGGCCAAAAGCATTCCCTCTCATTTTTATTGTTGAAGGGTGTAAGTATGAAGCAAAAACGGc                                                    <  1:1276715/140‑1 (MQ=255)                                                                                tcGCCGCCAGTTTGCCCATCACGGAGATATCGTCCAGTAGTGTGGCGATATCATCGATCAACGTCAGTAAACTACTCCCGGCCAAAAGCATTCCCTCTCATTTTTATTGTTGAAGGGTGTAAGTATGAAGCAAAAACGGc                                                    <  1:371795/140‑1 (MQ=255)                                                                                tcGCCGCCAGTTTGCCCATCACGGAGATATCGTCCAGTAGTGTGGCGATATCATCGATCAACGTCAGTAAACTACTCCCGGCCAAAAGCATTCCCTCTCATTTTTATTGTTGAAGGGTGTAAGTATGAAGCAAAAACGGc                                                    <  1:769684/140‑1 (MQ=255)                                                                                tcGCCGCCAGTTTGCCCATCACGGAGATATCGTCCAGTAGTGTGGCGATATCATCGATCAACGTCAGTAAACTACTCCCGGCCAAAAGCATTCCCTCTCATTTTTATTGTTGAAGGGTGTAAGTATGAAGCAAAAACGGc                                                    <  2:1289336/140‑1 (MQ=255)                                                                                tcGCCGCCAGTTTGCCCATCACGGAGATATCGTCCAGTAGTGTGGCGATATCATCGATCAACGTCAGTAAACTACTCCCGGCCAAAAGCATACCCTCTCATTTTTATTGTTGAAGGGTGTAAGTATGAAGCAAAAACGGc                                                    <  2:478767/140‑1 (MQ=255)                                                                                      ccAGTTTGCCCATCACGGAGATATCGTCCAGTAGTGTGGCGATATCATCGATCAACGTCAGTAAACTACTCCCGGCCAAAAGCATTCCCTCTCATTTTTATTGTTGAAGGGTGTAAGTATGAAGCAAAAACGGCAGctct                                              <  1:946751/140‑1 (MQ=255)                                                                                         gTTTGCCCATCACGGAGATATCGTCCAGTAGTGTGGCGATATCATCGATCAACGTCAGTAAACTACTCCCGGCCAAAAGCATTCCCTCTCATTTTTATTGTTGAAGGGTGTAAGTATGAAGCAAAAACGGCAGCTCTGAt                                           <  1:70291/140‑1 (MQ=255)                                                                                            tGCCCATCACGGAGATATCGTCCAGTAGTGTGGCGATATCATCGATCAACGTCAGTAAACTACTCCCGGCCAAAAGCATTCCCTCTCATTTTTATTGTTGAAGGGTGTAAGTATGAAGCAAAAACGGCAGCTCTGATACt                                        <  1:1212849/140‑1 (MQ=255)                                                                                                   cACGGAGATATCGTCCAGTAGTGTGGCGATATCATCGATCAACGTCAGTAAACTACTCCCGGCCAAAAGCATTCTCTCTCATTTTTATTGTTGAAGGGTGTAAGTATGAAGCAAAAACGGCAGCTCTGATACTTGCTTca                                 <  2:1182682/140‑1 (MQ=255)                                                                                                   cACGGAGATATCGTCCAGTAGTGTGGCGATATCATCGATCAACGTCAGTAAACTACTCCCGGCCAAAAGCATTCCCTCTCATTTTTATTGTTGAAGGGTGTAAGTATGAAGCAAAAACGGCAGCTCTGATACTTGCTTca                                 >  2:1282614/1‑140 (MQ=255)                                                                                                      ggAGATATCGTCCAGTAGTGTGGCGATATCATCGATCAACGTCAGTAAACTACTCCCGGCCAAAAGCATTCCCTCTCATTTTTATTGTTGAAGGGTGTAAGTATGAAGCAAAAACGGCAGCTCTGATACTTGCTTCACAg                              >  2:519848/1‑140 (MQ=255)                                                                                                            atCGTCCAGTAGTGTGGCGATATCATCGATCAACGTCAGTAAACTACTCCCGGCCAAAAGCATTCCCTCTCATTTTTATTGTTGAAGGGTGTAAGTATGAAGCAAAAACGGCAGCTCTGATACTTGCTTCACAGGTCAAc                        <  2:506628/140‑1 (MQ=255)                                                                                                             tCGTCCAGTAGTGTGGCGATATCATCGATCAACGTCAGTAAACTACTCCCGGCCAAAAGCATTCCCTCTCATTTTTATTGTTGAAGGGTGTAAGTATGAAGCAAAAACGGCAGCTCTGATACTTGCTTCACAGGTCAACa                       >  2:1218067/1‑140 (MQ=255)                                                                                                             tCGTCCAGTAGTGTGGCGATATCATCGATCAACGTCAGTAAACTACTCCCGGCCAAAAGCATTCCCTCTCATTTTTATTGTTGAAGGGTGTAAGTATGAAGCAAAAACGGCAGCTCTGATACTTGCTTCACAGGTCAACa                       >  2:371387/1‑140 (MQ=255)                                                                                                                tCCAGTAGTGTGGCGATATCATCGATCAACG‑CAGTAAACTACTCCCGGCCAAAAGCATTCCCTCTCATTTTTATTGTTGAAGGGTGTAAGTATGAAGCAAAAACGGCAGCTCTGATACTTGCTTCACAGGTCAACAtttt                   >  1:422872/1‑140 (MQ=255)                                                                                                                 ccAGTAGTGTGGCGATATCATCGATCAACGTCAGTAAACTACTCCCGGCCAAAAGCATTCCCTCTCATTTTTATTGTTGAAGGGTGTAAGTATGAAGCAAAAACGGCAGCTCTGATACTTGCTTCACAGGTCAACAtttt                   <  2:279988/140‑1 (MQ=255)                                                                                                                   agtagtGTGGCGATATCATCGATCAACGTCAGTAAACTACTCCCGGCCAAAAGCATTCCCTCTCATTTTTATTGTTGAAGGGTGTAAGTATGAAGCAAAAACGGCAGCTCTGATACTTGCTTCACAGGTCAACAtttttt                 <  2:393970/140‑1 (MQ=255)                                                                                                                   agtagtGTGGCGATATCATCGATCAACGTCAGTAAACTACTCCCGGCCAAAAGCATTCCCTCTCATTTTTATTGTTGAAGGGTGTAAGTATGAAGCAAAAACGGCAGCTCTGATACTTGCTTCACAGGTCAACAtttttt                 <  1:904924/140‑1 (MQ=255)                                                                                                                    gtagtGTGGCGATATCATCGATCAACGTCAGTAAACTACTCCCGGCCAAAAGCATTCCCTCTCATTTTTATTGTTGAAGGGTGTAAGTATGAAGCAAAAACGGCAGCTCTGATACTTGCTTCACAGGTCAACAttttttt                >  1:189072/1‑140 (MQ=255)                                                                                                                     tagtGTGGCGATATCATCGATCAACGTCAGTAAACTACTCCCGGCCAAAAGCATTCCCTCTCATTTTTATTGTTGAAGGGTGTAAGTATGAAGCAAAAACGGCAGCTCTGATACTTGCTTCACAGGTCAACATTTTTTTa               >  2:1189160/1‑140 (MQ=255)                                                                                                                      agtGTGGCGATATCATCGATCAACGTCAGTAAACTACTCCCGGCCAAAAGCATTCCCTCTCATTTTTATTGTTGAAGGGTGTAAGTATGAAGCAAAAACGGCAGCTCTGATACTTGCTTCACAGGTCAACATTTTTTTaa              <  1:253751/140‑1 (MQ=255)                                                                                                                        tgtgGCGATATCATCGATCAACGTCAGTAAACTACTCCCGGCCAAAAGCATTCCCTCTCATTTTTATTGTTGAAGGGTGTAAGTATGAAGCAAAAACGGCAGCTCTGATACTTGCTTCACAGGTCAACATTTTTTTAACa            >  2:1162196/1‑140 (MQ=255)                                                                                                                        tgtgGCGATATCATCGATCAACGTCAGTAAACTACTCCCGGCCAAAAGCATTCCCTCTCATTTTTATTGTTGAAGGGTGTAAGTATGAAGCAAAAACGGCAGCTCTGATACTTGCTTCACAGGTCAACATTTTTTTAACa            >  2:169363/1‑140 (MQ=255)                                                                                                                         gtgGCGATATCATCGATCAACGTCAGTAAACTACTCCCGGCCAAAAGCATTCCCTCTCATTTTTATTGTTGAAGGGTGTAAGTATGAAGCAAAAACGGCAGCTCTGATACTTGCTTCACAGGTCAACATTTTTTTAACaa           <  2:1018297/140‑1 (MQ=255)                                                                                                                         gtgGCGATATCATCGATCAACGTCAGTAAACTACTCCCGGCCAAAAGCATTCCCTCTCATTTTTATTGTTGAAGGGTGTAAGTATGAAGCAAAAACGGCAGCTCTGATACTTGCTTCACAGGTCAACATTTTTTTAACaa           <  2:449035/140‑1 (MQ=255)                                                                                                                          tgGCGATATCATCGATCAACGTCAGTAAACTACTCCCGGCCAAAAGCATTCCCTCTCATTTTTATTGTTGAAGGGTGTAAGTATGAAGCAAAAACGGCAGCTCTGATACTTGCTTCACAGGTCAACATTTTTTTAACAAt          >  2:340640/1‑140 (MQ=255)                                                                                                                           ggCGATATCATCGATCAACGTCAGTAAACTACTCCCGGCCAAAAGCATTCCCTCTCATTTTTATTGTTGAAGGGTGTAAGTATGAAGCAAAAACGGCAGCTCTGATACTTGCTTCACAGGTCAACATTTTTTTAACAATc         <  1:754260/140‑1 (MQ=255)                                                                                                                           ggCGATATCATCGATCAACGTCAGTAAACTACTCCCGGCCAAAAGCATTCCCTCTCATTTTTATTGTTGAAGGGTGTAAGTATGAAGCAAAAACGGCAGCTCTGATACTTGCTTCACAGGTCAACATTTTTTTAACAATc         >  1:185221/1‑140 (MQ=255)                                                                                                                            gCGATATCATCGATCAACGTCAGTAAACTACTCCCGGCCAAAAGCATTCCCTCTCATTTTTATTGTTGAAGGGTGTAAGTATGAAGCAAAAACGGCAGCTCTGATACTTGCTTCACAGGTCAACATTTTTTTAACAATCa        <  1:1162196/140‑1 (MQ=255)                                                                                                                               atatCATCGATCAACGTCAGTAAACTACTCCCGGCCAAAAGCATTCCCTCTCATTTTTATTGTTGAAGGGTGTAAGTATGAAGCAAAAACGGCAGCTCTGATACTTGCTTCACAGGTCAACATTTTTTTAACAATCAAGt     >  2:541828/1‑140 (MQ=255)                                                                                                                                tatCATCGATCAACGTCAGTAAACTACTCCCGGCCAAAAGCATTCCCTCTCATTTTTATTGTTGAAGGGTGTAAGTATGAAGCAAAAACGGCAGCTCTGATACTTGCTTCACAGGTCAACATTTTTTTAACAATCAAGTg    <  1:415851/140‑1 (MQ=255)                                                                                                                                 atcatcGATCAACGTCAGTAAACTACTCCCGGCCAAAAGCATTCCCTCTCATTTTTATTGTTGAAGGGTGTAAGTATGAAGCAAAAACGGCAGCTCTGATACTTGCTTCACAGGTCAACATTTTTTTAACAATCAAGTGc   >  1:939844/1‑140 (MQ=255)                                                                                                                                  tcatcGATCAACGTCAGTAAACTACTCCCGGCCAAAAGCATTCCCTCTCATTTTTATTGTTGAAGGGTGTAAGTATGAAGCAAAAACGGCAGCTCTGATACTTGCTTCACAGGTCAACATTTTTTTAACAATCAAGTGCa  >  1:1042364/1‑140 (MQ=255)                                                                                                                                  tcatcGATCAACGTCAGTAAACTACTCCCGGCCAAAAGCATTCCCTCTCATTTTTATTGTTGAAGGGTGTAAGTATGAAGCAAAAACGGCAGCTCTGATACTTGCTTCACAGGTCAACATTTTTTTAACAATCAAGTGCa  >  2:99878/1‑140 (MQ=255)                                                                                                                                         |                                                                                                                                     AGTTCCCGGTTGGCCCGCACGCCTGAAACTTGTTGCGCATTGAGCGATAAGTCATCCCCTAATACACCGGCGGTTTTCTTCGCCGCCAGTTTGCCCATCACGGAGATATCGTCCAGTAGTGTGGCGATATCATCGAGCAACGTCAGTAAACTACTCCCGGCCAAAAGCATTCCCTCTCATTTTTATTGTTGAAGGGTGTAAGTATGAAGCAAAAACGGCAGCTCTGATACTTGCTTCACAGGTCAACATTTTTTTAACAATCAAGTGCA  >  NC_000913/2029197‑2029465

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑