Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,324,606	G→T	M166I (ATG→ATT)	atoA →	acetyl‑CoA:acetoacetyl‑CoA transferase subunit beta

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,324,606	0	G	T	100.0%	211.5 / NA	61	M166I (ATG→ATT)	atoA	acetyl‑CoA:acetoacetyl‑CoA transferase subunit beta
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (35/26);  total (35/26)

GGCGCGATGGATCTGGTGACCGGGTCGCGCAAAGTGATCATCGCCATGGAACATTGCGCCAAAGATGGTTCAGCAAAAATTTTGCGCCGCTGCACCATGCCACTCACTGCGCAACATGCGGTGCATATGCTGGTTACTGAACTGGCTGTCTTTCGTTTTATTGACGGCAAAATGTGGCTCACCGAAATTGCCGACGGGTGTGATTTAGCCACCGTGCGTGCCAAAACAGAAGCTCGGTTTGAAGTCGCCGCCGATCTGAATACG  >  NC_000913/2324478‑2324741                                                                                                                                 |                                                                                                                                        ggCGCGATGGATCTGGTGACCGGGTCGCGCAAAGTGATCATCGCCATGGAACATTGCGCCAAAGATGGTTCAGCAAAAATTTTGCGCCGCTGCACCATGCCACTCACTGCGCAACATGCGGTGCATATTCTGGTTACTGa                                                                                                                              >  1:712575/1‑140 (MQ=255) ggCGCGATGGATCTGGTGACCGGGTCGCGCAAAGTGATCATCGCCATGGAACATTGCGCCAAAGATGGTTCAGCAAAAATTTTGCGCCGCTGCACCATGCCACTCACTGCGCAACATGCGGTGCATATTCTGGTTACTGa                                                                                                                              <  2:687027/140‑1 (MQ=255)   cgcgATGGATCTGGTGACCGGGTCGCGCAAAGTGATCATCGCCATGGAACATTGCGCCAAAGATGGTTCAGCAAAAATTTTGCGCCGCTGCACCATGCCACTCACTGCGCAACATGCGGTGCATATTCTGGTTACTGAAc                                                                                                                            <  1:630122/140‑1 (MQ=255)   cgcgATGGATCTGGTGACCGGGTCGCGCAAAGTGATCATCGCCATGGAACATTGCGCCAAAGATGGTTCAGCAAAAATTTTGCGCCGCTGCACCATGCCACTCACTGCGCAACATGCGGTGCATATTCTGGTTACTGAAc                                                                                                                            >  2:573500/1‑140 (MQ=255)   cgcgATGGATCTGGTGACCGGGTCGCGCAAAGTGATCATCGCCATGGAACATTGCGCCAAAGATGGTTCAGCAAAAATTTTGCGCCGCTGCACCATGCCACTCACTGCGCAACATGCGGTGCATATTCTGGTTACTGAAc                                                                                                                            >  1:464050/1‑140 (MQ=255)   cgcgATGGATCTGGTGACCGGGTCGCGCAAAGTGATCATCGCCATGGAACATTGCGCCAAAGATGGCTCAGCAAAAATTTTGCGCCGCTGCACCATGCCACTCACTGCGCAACATGCGGTGCATATTCTGGTTACTGAAc                                                                                                                            >  1:216469/1‑140 (MQ=255)             cTGGTGACCGGGTCGCGCAAAGTGATCATCGCCATGGAACATTGCGCCAAAGATGGTTCAGCAAAAATTTTGCGCCGCTGCACCATGCCACTCACTGCGCAACATGCGGTGCATATTCTGGTTACTGAACTGGCTGTCtt                                                                                                                  <  2:464050/140‑1 (MQ=255)             cTGGTGACCGGGTCGCGCAAAGTGATCATCGCCATGGAACATTGCGCCAAAGATGGTTCAGCAAAAATTTTGCGCCGCTGCACCATGCCACTCACTGCGCAACATGCGGTGCATATTCTGGTTACTGAACTGGCTGTCtt                                                                                                                  <  1:1145874/140‑1 (MQ=255)                  gACCGGGTCGCGCAAAGTGATCATCGCCATGGAACATTGCGCCAAAGATGGTTCAGCAAAAATTTTGCGCCGCTGCACCATGCCACTCACTGCGCAACATGCGGTGCATATTCTGGTTACTGAACTGGCTGTCTTTCGtt                                                                                                             >  2:884814/1‑140 (MQ=255)                    ccGGGTCGCGCAAAGTGATCATCGCCATGGAACATTGCGCCAAAGATGGTTCAGCAAAAATTTTGCGCCGCTGCACCATGCCACTCACTGCGCAACATGCGGTGCATATTCTGGTTACTGAACTGGCTGTCTTTCGtttt                                                                                                           >  2:1307207/1‑140 (MQ=255)                     cGGGTCGCGCAAAGTGATCATCGCCATGGAACATTGCGCCAAAGATGGTTCAGCAAAAATTTTGCGCCGCTGCACCATGCCACTCACTGCGCAACATGCGGTGCATATTCTGGTTACTGAACTGGCTGTCTTTCGTTTTa                                                                                                          >  1:1066902/1‑140 (MQ=255)                       ggTCGCGCAAAGTGATCATCGCCATGGAACATTGCGCCAAAGATGGTTCAGCAAAAATTTTGCGCCGCTGCACCATGCCACTCACTGCGCAACATGCGGTGCATATTCTGGTTACTGAACTGGCTGTCTTTCGTTTTAtt                                                                                                        >  2:801364/1‑140 (MQ=255)                       ggTCGCGCAAAGTGATCATCGCCATGGAACATTGCGCCAAAGATGGTTCAGCAAAAATTTTGCGCCGCTGCACCATGCCACTCACTGCGCAACATGCGGTGCATATTCTGGTTACTGAACTGGCTGTCTTTCGTTTTAtt                                                                                                        >  2:10648/1‑140 (MQ=255)                        gTCGCGCAAAGTGATCATCGCCATGGAACATTGCGCCAAAGATGGTTCAGCAAAAATTTTGCGCCGCTGCACCATGCCACTCACTGCGCAACATGCGGTGCATATTCTGGTTACTGAACTGGCTGTCTTTCGTTTTATTg                                                                                                       >  2:123088/1‑140 (MQ=255)                         tCGCGCAAAGTGATCATCGCCATGGAACATTGCGCCAAAGATGGTTCAGCAAAAATTTTGCGCCGCTGCACCATGCCACTCACTGCGCAACATGCGGTGCATATTCTGGTTACTGAACTGGCTGTCTTTCGTTTTATTGa                                                                                                      <  1:842012/140‑1 (MQ=255)                            cgcAAAGTGATCATCGCCATGGAACATTGCGCCAAAGATGGTTCAGCAAAAATTTTGCGCCGCTGCACCATGCCACTCACTGCGCAACATGCGGTGCATATTCTGGTTACTGAACTGGCTGTCTTTCGTTTTATTGACgg                                                                                                   <  1:1304519/140‑1 (MQ=255)                            cgcAAAGTGATCATCGCCATGGAACATTGCGCCAAAGATGGTTCAGCAAAAATTTTGCGCCGCTGCACCATGCCACTCACTGCGCAACATGCGGTGCATATTCTGGTTACTGAACTGGCTGTCTTTCGTTTTATTGACgg                                                                                                   <  2:1211621/140‑1 (MQ=255)                               aaaGTGATCATCGCCATGGAACATTGCGCCAAAGATGGTTCAGCAAAAATTTTGCGCCGCTGCACCATGCCACTCACTGCGCAACATGCGGTGCATATTCTGGTTACTGAACTGGCTGTCTTTCGTTTTATTGACGGCaa                                                                                                >  1:360685/1‑140 (MQ=255)                                   tGATCATCGCCATGGAACATTGCGCCAAAGATGGTTCAGCAAAAATTTTGCGCCGCTGCACCATGCCACTCACTGCGCAACATGCGGTGCATATTCTGGTTACTGAACTGGCTGTCTTTCGTTTTATTGACGGCAAAAtg                                                                                            >  1:497820/1‑140 (MQ=255)                                           gCCATGGAACATTGCGCCAAAGATGGTTCAGCAAAAATTTTGCGCCGCTGCACCATGCCACTCACTGCGCAACATGCGGTGCATATTCTGGTTACTGAACTGGCTGTCTTTCGTTTTATTGACGGCAAAATGTGGCTCAc                                                                                    >  2:769222/1‑140 (MQ=255)                                             cATGGAACATTGCGCCAAAGATGGTTCAGCAAAAATTTTGCGCCGCTGCACCATGCCACTCACTGCGCAACATGCGGTGCATATTCTGGTTACTGAACTGGCTGTCTTTCGTTTTATTGACGGCAAAATGTGGCTCACCg                                                                                  >  1:382875/1‑140 (MQ=255)                                             cATGGAACATTGCGCCAAAGATGGTTCAGCAAAAATTTTGCGCCGCTGCACCATGCCACTCACTGCGCAACATGCGGCGCATATTCTGGTTACTGAACTGGCTGTCTTTCGTTTTATTGACGGCAAAATGTGGCTCACCg                                                                                  >  2:912228/1‑140 (MQ=255)                                              aTGGAACATTGCGCCAAAGATGGTTCAGCAAAAATTTTGCGCCGCTGCACCATGCCACTCACTGCGCAACATGCGGTGCATATTCTGGTTACTGAACTGGCTGTCTTTCGTTTTATTGACGGCAAAATGTGGCTCACCGa                                                                                 <  2:1258339/140‑1 (MQ=255)                                               tGGAACATTGCGCCAAAGATGGTTCAGCAAAAATTTTGCGCCGCTGCACCATGCCACTCACTGCGCAACATGCGGTGCATATTCTGGTTACTGAACTGGCTGTCTTTCGTTTTATTGACGGCAAAATGTGGCTCACCGaa                                                                                >  2:538896/1‑140 (MQ=255)                                               tGGAACATTGCGCCAAAGATGGTTCAGCAAAAATTTTGCGCCGCTGCACCATGCCACTCACTGCGCAACATGCGGTGCATATTCTGGTTACTGAACTGGCTGTCTTTCGTTTTATTGACGGCAAAATGTGGCTCACCGaa                                                                                >  1:1301323/1‑140 (MQ=255)                                                ggAACATTGCGCCAAAGATGGTTCAGCAAAAATTTTGCGCCGCTGCACCATGCCACTCACTGCGCAACATGCGGTGCATATTCTGGTTACTGAACTGGCTGTCTTTCGTTTTATTGACGGCAAAATGTGGCTCACCGaaa                                                                               >  1:685151/1‑140 (MQ=255)                                                  aaCATTGCGCCAAAGATGGTTCAGCAAAAATTTTGCGCCGCTGCACCATGCCACTCACTGCGCAACATGCGGTGCATATTCTGGTTACTGAACTGGCTGTCTTTCGTTTTATTGACGGCAAAATGTGGCTCACCGAAAtt                                                                             <  2:382875/140‑1 (MQ=255)                                                    cATTGCGCCAAAGATGGTTCAGCAAAAATTTTGCGCCGCTGCACCATGCCACTCACTGCGCAACATGCGGTGCATATTCTGGTTACTGAACTGGCTGTCTTTCGTTTTATTGACGGCAAAATGTGGCTCACCGAAATTGc                                                                           >  2:320044/1‑140 (MQ=255)                                                     aTTGCGCCAAAGATGGTTCAGCAAAAATTTTGCGCCGCTGCACCATGCCACTCACTGCGCAACATGCGGTGCATATTCTGGTTACTGAACTGGCTGTCTTTCGTTTTATTGACGGCAAAATGTGGCTCACCGAAATTGcc                                                                          <  2:372373/140‑1 (MQ=255)                                                     aTTGCGCCAAAGATGGTTCAGCAAAAATTTTGCGCCGCTGCACCATGCCACTCACTGCGCAACATGCGGTGCATATTCTGGTTACTGAACTGGCTGTCTTTCGTTTTATTGACGGCAAAATGTGGCTCACCGAAATTGcc                                                                          <  2:82702/140‑1 (MQ=255)                                                      ttGCGCCAAAGATGGTTCAGCAAAAATTTTGCGCCGCTGCACCATGCCACTCACTGCGCAACATGCGGTGCATATTCTGGTTACTGAACTGGCTGTCTTTCGTTTTATTGACGGCAAAATGTGGCTCACCGAAATTGCCg                                                                         <  2:962454/140‑1 (MQ=255)                                                      ttGCGCCAAAGATGGCTCAGCAAAAATTTTGCGCCGCTGCACCATGCCACTCACTGCGCAACATGCGGTGCATATTCTGGTTACTGAACTGGCTGTCTTTCGTTTTATTGACGGCAAAATGTGGCTCACCGAAATTGCCg                                                                         <  2:216469/140‑1 (MQ=255)                                                       tGCGCCAAAGATGGTTCAGCAAAAATTTTGCGCCGCTGCACCATGCCACTCACTGCGCAACATGCGGTGCATATTCTGGTTACTGAACTGGCTGTCTTTCGTTTTATTGACGGCAAAATGTGGCTCACCGAAATTGCCGa                                                                        <  1:573500/140‑1 (MQ=255)                                                       tGCGCCAAAGATGGTTCAGCAAAAATTTTGCGCCGCTGCACCATGCCACTCACTGCGCAACATGCGGTGCATATTCTGGTTACTGAACTGGCTGTCTTTCGTTTTATTGACGGCAAAATGTGGCTCACCGAAATTGCCGa                                                                        >  2:532078/1‑140 (MQ=255)                                                       tGCGCCAAAGATGGTTCAGCAAAAATTTTGCGCCGCTGCACCATGCCACTCACTGCGCAACATGCGGTACATATTCTGGTTACTGAACTGGCTGTCTTTCGTTTTATTGACGGCAAAATGTGGCTCACCGAAATTGCCGa                                                                        <  2:339800/140‑1 (MQ=255)                                                         cgcCAAAGATGGTTCAGCAAAAATTTTGCGCCGCTGCACCATGCCACTCACTGCGCAACATGCGGTGCATATTCTGGTTACTGAACTGGCTGTCTTTCGTTTTATTGACGGCAAAATGTGGCTCACCGAAATTGCCGACg                                                                      >  2:1305735/1‑140 (MQ=255)                                                           ccAAAGATGGTTCAGCAAAAATTTTGCGCCGCTGCACCATGCCACTCACTGCGCAACATGCGGTGCATATTCTGGTTACTGAACTGGCTGTCTTTCGTTTTATTGACGGCAAAATGTGGCTCACCGAAATTGCCGACggg                                                                    >  1:1165263/1‑140 (MQ=255)                                                            cAAAGATGGTTCAGCAAAAATTTTGCGCCGCTGCACCATGCCACTCACTGCGCAACATGCGGTGCATATTCTGGTTACTGAACTGGCTGTCTTTCGTTTTATTGACGGCAAAATGTGGCTCACCGAAATTGCCGACGGgt                                                                   <  2:360685/140‑1 (MQ=255)                                                            cAAAGATGGTTCAGCAAAAATTTTGCGCCGCTGCACCATGCCACTCACTGCGCAACATGCGGTGCATATTCTGGTTACTGAACTGGCTGTCTTTCGTTTTATTGACGGCAAAATGTGGCTCACCGAAATTGCCGACGGgt                                                                   <  1:538896/140‑1 (MQ=255)                                                              aaGATGGTTCAGCAAAAATTTTGCGCCGCTGCACCATGCCACTCACTGCGCAACATGCGGTGCATATTCTGGTTACTGAACTGGCTGTCTTTCGTTTTATTGACGGCAAAATGTGGCTCACCGAAATTGCCGACGGgtgt                                                                 <  2:748341/140‑1 (MQ=255)                                                                      tCAGCAAAAATTTTGCGCCGCTGCACCATGCCACTCACTGCGCAACATGCGGTGCATATTCTGGTTACTGAACTGGCTGTCTTTCGTTTTATTGACGGCAAAATGTGGCTCACCGAAATTGCCGACGGGTGTGATTTAGc                                                         <  2:359813/140‑1 (MQ=255)                                                                      tCAGCAAAAATTTTGCGCCGCTGCACCATGCCACTCACTGCGCAACATGCGGTGCATATTCTGGTTACTGAACTGGCTGTCTTTCGTTTTATTGACGGCAAAATGTGGCTCACCGAAATTGCCGACGGGTGTGATTTAGc                                                         >  2:383386/1‑140 (MQ=255)                                                                        aGCAAAAATTTTGCGCCGCTGCACCATGCCACTCACTGCGCAACATGCGGTGCATATTCTGGTTACTGAACTGGCTGTCTTTCGTTTTATTGACGGCAAAATGTGGCTCACCGAAATTGCCGACGGGTGTGATTTAGCCa                                                       <  1:999867/140‑1 (MQ=255)                                                                        aGCAAAAATTTTGCGCCGCTGCACCATGCCACTCACTGCGCAACATGCGGTGCATATTCTGGTTACTGAACTGGCTGTCTTTCGTTTTATTGACGGCAAAATGTGGCTCACCGAAATTGCCGACGGGTGTGATTTAGCCa                                                       >  1:274191/1‑140 (MQ=255)                                                                           aaaaaTTTTGCGCCGCTGCACCATGCCACTCACTGCGCAACATGCGGTGCATATTCTGGTTACTGAACTGGCTGTCTTTCGTTTTATCGACGGCAAAATGTGGCTCACCGAAATTGCCGACGGGTGTGATTTAGCCACcg                                                    >  2:730560/1‑140 (MQ=255)                                                                             aaaTTTTGCGCCGCTGCACCATGCCACTCACTGCGCAACACGCGGTGCATATTCTGGTTACTGAACTGGCTGTCTTTCGTTTTATTGACGGCAAAATGTGGCTCACCGAAATTGCCGACGGGTGTGATTTAGCCACcgtg                                                  >  1:832639/1‑140 (MQ=255)                                                                                    gcgcCGCTGCACCATGCCACTCACTGCGCAACATGCGGTGCATATTCTGGTTACTGAACTGGCTGTCTTTCGTTTTATTGACGGCAAAATGTGGCTCACCGAAATTGCCGACGGGTGTGATTTAGCCACCGTGCGTGCCa                                           >  2:1020188/1‑140 (MQ=255)                                                                                          cTGCACCATGCCACTCACTGCGCAACATGCGGTGCATATTCTGGTTACTGAACTGGCTGTCTTTCGTTTTATTGACGGCAAAATGTGGCTCACCGAAATTGCCGACGGGTGTGATTTAGCCACCGTGCGTGCCAAAACAg                                     <  2:1301323/140‑1 (MQ=255)                                                                                                  tGCCACTCACTGCGCAACATGCGGTGCATATTCTGGTTACTGAACTGGCTGTCTTTCGTTTTATTGACGGCAAAATGTGGCTCACCGAAATTGCCGACGGGTGTGATTTAGCCACCGTGCGTGCCAAAACAGAAGCTCgg                             >  1:805939/1‑140 (MQ=255)                                                                                                  tGCCACTCACTGCGCAACATGCGGTGCATATTCTGGTTACTGAACTGGCTGTCTTTCGTTTTATTGACGGCAAAATGTGGCTCACCGAAATTGCCGACGGGTGTGATTTAGCCACCGTGCGTGCCAAAACAGAAGCTCgg                             >  1:732737/1‑140 (MQ=255)                                                                                                    ccACTCACTGCGCAACATGCGGTGCATATTCTGGTTACTGAACTGGCTGTCTTTCGTTTTATTGACGGCAAAATGTGGCTCACCGAAATTGCCGACGGGTGTGATTTAGCCACCGTGCGTGCCAAAACAGAAGCTCGGtt                           >  1:893767/1‑140 (MQ=255)                                                                                                          actGCGCAACATGCGGTGCATATTCTGGTTACTGAACTGGCTGTCTTTCGTTTTATTGACGGCAAAATGTGGCTCACCGAAATTGCCGACGGGTGTGATTTAGCCACCGTGCGTGCCAAAACAGAAGCTCGGTTTGAAGt                     >  1:1127324/1‑140 (MQ=255)                                                                                                              cgcAACATGCGGTGCATATTCTGGTTACTGAACTGGCTGTCTTTCGTTTTATTGACGGCAAAATGTGGCTCACCGAAATTGCCGACGGGTGTGATTTAGCCACCGTGCGTGCCAAAACAGAAGCTCGGTTTGAAGTcgcc                 >  2:280574/1‑140 (MQ=255)                                                                                                              cgcAACATGCGGTGCATATTCT‑GTTACTGAACTGGCTGTCTTTCGTTTTATTGACGGCAAAATGTGGCTCACCGAAATTGCCGACGGGTGTGATTTAGCCACCGTGCGTGCCAAAACAGAAGCTCGGTTTGAAGTcgccg                >  2:302226/1‑140 (MQ=255)                                                                                                               gcAACATGCGGTGCATATTCTGGTTACTGAACTGGCTGTCTTTCGTTTTATTGACGGCAAAATGTGGCTCACCGAAATTGCCGACGGGTGTGATTTAGCCACCGTGCGTGCCAAAACAGAAGCTCGGTTTGAAGTcgccg                <  1:117931/140‑1 (MQ=255)                                                                                                               gcAACATGCGGTGCATATTCTGGTTACTGAACTGGCTGTCTTTCGTTTTATTGACGGCAAAATGTGGCTCACCGAAATTGCCGACGGGTGTGATTTAGCCACCGTGCGTGCCAAAACAGAAGCTCGGTTTGAAGTcgccg                <  1:10648/140‑1 (MQ=255)                                                                                                                   cATGCGGTGCATATTCTGGTTACTGAACTGGCTGTCTTTCGTTTTATTGACGGCAAAATGTGGCTCACCGAAATTGCCGACGGGTGTGATTTAGCCACCGTGCGTGCCAAAACAGAAGCTCGGTTTGAAGTCGCCGCCGa            <  2:87157/140‑1 (MQ=255)                                                                                                                     tGCGGTGCATATTCTGGTTACTGAACTGGCTGTCTTTCGTTTTATTGACGGCAAAATGTGGCTCACCGAAATTGCCGACGGGTGTGATTTAGCCACCGTGCGTGCCAAAACAGAAGCTCGGTTTGAAGTCGCCGCCGATc          <  1:123088/140‑1 (MQ=255)                                                                                                                      gCGGTGCATATTCTGGTTACTGAACTGGCTGTCTTTCGTTTTATTGACGGCAAAATGTGGCTCACCGAAATTGCCGACGGGTGTGATTTAGCCACCGTGCGTGCCAAAACAGAAGCTCGGTTTGAAGTCGCCGCCGATCt         >  2:626111/1‑140 (MQ=255)                                                                                                                      gCGGTGCATATTCTGGTTACTGAACTGGCTGTCTTTCGTTTTATTGACGGCAAAATGTGGCTCACCGAAATTGCCGACGGGTGTGATTTAGCCACCGTGCGTGCCAAAACAGAAGCTCGGTTTGAAGTCGCCGCCGATCt         >  2:1246592/1‑140 (MQ=255)                                                                                                                      gCGGTGCATATTCTGGTTACTGAACTGGCTGTCTTTCGTTTTATTGACGGCAAAATGTGGCTCACCGAAATTGCCGACGGGTGTGATTTAGCCACCGTGCGTGCCAAAACAGAAGCTCGGTTTGAAGTCGCCGCCGATCt         >  2:687293/1‑140 (MQ=255)                                                                                                                             atatTCTGGTTACTGAACTGGCTGTCTTTCGTTTTATTGACGGCAAAATGTGGCTCACCGAAATTGCCGACGGGTGTGATTTAGCCACCGTGCGTGCCAAAACAGAAGCTCGGTTTGAAGTCGCCGCCGATCTGAATACg  <  1:944556/140‑1 (MQ=255)                                                                                                                                 |                                                                                                                                        GGCGCGATGGATCTGGTGACCGGGTCGCGCAAAGTGATCATCGCCATGGAACATTGCGCCAAAGATGGTTCAGCAAAAATTTTGCGCCGCTGCACCATGCCACTCACTGCGCAACATGCGGTGCATATGCTGGTTACTGAACTGGCTGTCTTTCGTTTTATTGACGGCAAAATGTGGCTCACCGAAATTGCCGACGGGTGTGATTTAGCCACCGTGCGTGCCAAAACAGAAGCTCGGTTTGAAGTCGCCGCCGATCTGAATACG  >  NC_000913/2324478‑2324741

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑