Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,453,155	G→T	P39Q (CCG→CAG)	rpsJ ←	30S ribosomal subunit protein S10

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,453,155	0	G	T	100.0%	197.2 / NA	56	P39Q (CCG→CAG)	rpsJ	30S ribosomal subunit protein S10
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (31/25);  total (31/25)

CAACGGTTTTCTCGGTTGGCTCAACGATGTCAACCAGACGCAAGTGAGTACGGATTTCGTACTGATCGCGCGCGTCTTTGTTGACGTGCGGGGAGATCAGAACAGTGAAGCGCTCTTTGCGTGTCGGCAGCGGGATCGGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGTTGCTTGATCGATCAGACGATGATCAAACGCTTTCAGGCGGATACGGATTCTTTGGTTCTGCATGAGACCAGAGCTCCAATTA  >  NC_000913/3453018‑3453289                                                                                                                                          |                                                                                                                                       cAACGGTTTTCTCGGTTGGCTCAACGATGTCAACCAGACGCAAGTGAGTACGGATTTCGTACTGATCGCGCGCGTCTTTGTTGACGTGCGGGGAGATCAGAACAGTGAAGAGCTCTTTGCGTGTCGGCAGCGGGATCTGa                                                                                                                                      >  1:1033276/1‑140 (MQ=255)   aCGGTTTTCTCGGTTGGCTCAACGATGTCAACCAGACGCAAGTGAGTACGGATTTCGTACTGATCGCGCGCGTCTTTGTTGACGTGCGGGGAGATCAGAACAGTGAAGCGCTCTTTGCGTGTCGGCAGCGGGATCTGAcc                                                                                                                                    >  2:348536/1‑140 (MQ=255)       ttttCTCGGTTGGCTCAACGATGTCAACCAGACGCAAGTGAGTACGGATTTCGTACTGATCGCGCGCGTCTTTGTTGACGTGCGGGGAGATCAGAACAGTGAAGCGCTCTTTGCGTGTCGGCAGCGGGATCTGACCACgg                                                                                                                                <  2:488109/140‑1 (MQ=255)                  ggCTCAACGATGTCAACCAGACGCAAGTGAGTACGGATTTCGTACTGATCGCGCGCGTCTTTGTTGACGTGCGGGGAGATCAGAACAGTGAAGCGCTCTTTGCGTGTCGGCAGCGGGATCTGACCACGGACCTGCGCAcc                                                                                                                     >  2:445681/1‑140 (MQ=255)                   gCTCAACGATGTCAACCAGACGCAAGTGAGTACGGATTTCGTACTGATCGCGCGCGTCTTTGTTGACGTGCGGGGAGATCAGAACAGTGAAGCGCTCTTTGCGTGTCGGCAGCGGGATCTGACCACGGACCTgcg                                                                                                                         >  1:418797/1‑135 (MQ=255)                   gCTCAACGATGTCAACCAGACGCAAGTGAGTACGGATTTCGTACTGATCGCGCGCGTCTTTGTTGACGTGCGGGGAGATCAGAACAGTGAAGCGCTCTTTGCGTGTCGGCAGCGGGATCTGACCACGGACCTgcg                                                                                                                         <  2:418797/135‑1 (MQ=255)                     tCAACGATGTCAACCAGACGCAAGTGAGTACGGATTTCGTACTGATCGCGCGCGTCTTTGTTGACGTGCGGGGAGATCAGAACAGTGAAGCGCTCTTTGCGTGTCGGCAGCGGGATCTGACCACGGACCTGCGCACCAGt                                                                                                                  <  2:440859/140‑1 (MQ=255)                        aCGATGTCAACCAGACGCAAGTGAGTACGGATTTCGTACTGATCGCGCGCGTCTTTGTTGACGTGCGGGGAGATCAGAACAGTGAAGCGCTCTTTGCGTGTCGGCAGCGGGATCTGACCACGGACCTGCGCACCAGTgcg                                                                                                               >  1:641332/1‑140 (MQ=255)                               cAACCAGACGCAAGTGAGTACGGATTTCGTACTGATCGCGCGCGTCTTTGTTGACGTGCGGGGAGATCAGAACAGTGAAGCGCTCTTTGCGTGTCGGCAGCGGGATCTGACCACGGACCTGCGCACCAGTGCGCTTGGCa                                                                                                        <  2:1144785/140‑1 (MQ=255)                                 aCCAGACGCAAGTGAGTACGGATTTCGTACTGATCGCGCGCGTCTTTGTTGACGTGCGGGGAGATCAGAACAGTGAAGCGCTCTTTGCGTGTCGGCAGCGGGATCTGACCACGGACCTGCGCACCAGTGCGCTTGGCAGt                                                                                                      >  1:1047350/1‑140 (MQ=255)                                      aCGCAAGTGAGTACGGATTTCGTACTGATCGCGCGCGTCTTTGTTGACGTGCGGGGAGATCAGAACAGTGAAGCGCTCTTTGCGTGTCGGCAGCGGGATCTGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTcga                                                                                                 >  2:269541/1‑140 (MQ=255)                                        gCAAGTGAGTACGGATTTCGTACTGATCGCGCGCGTCTTTGTTGACGTGCGGGGAGATCAGAACAGTGAAGCGCTCTTTGCGTGTCGGCAGCGGGATCTGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTcgacg                                                                                               >  1:71464/1‑140 (MQ=255)                                           aGTGAGTACGGATTTCGTACTGATCGCGCGCGTCTTTGTTGACGTGCGGGGAGATCAGAACAGTGAAGCGCTCTTTGCGTGTCGGCAGCGGGATCTGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGAtt                                                                                            >  1:982960/1‑140 (MQ=255)                                            gTGAGTACGGATTTCGTACTGATCGCGCGCGTCTTTGTTGACGTGCGGGGAGATCAGAACAGTGAAGCGCTCTTTGCGTGTCGGCAGCGGGATCTGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGAttt                                                                                           >  1:1029478/1‑140 (MQ=255)                                                      aTTTCGTACTGATCGCGCGCGTCTTTGTTGACGTGCGGGGAGATCAGAACAGTGAAGCGCTCTTTGCGTGTCGGCAGCGGGATCTGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGTTGc                                                                                 >  1:391892/1‑140 (MQ=255)                                                         tCGTACTGATCGCGCGCGTCTTTGTTGACGTGCGGGGAGATCAGAACAGTGAAGCGCTCTTTGCGTGTCGGCAGCGGGATCTGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGTTGCTTg                                                                              >  2:593381/1‑140 (MQ=255)                                                           gTACTGATCGCGCGCGTCTTTGTTGACGTGCGGGGAGATCAGAACAGTGAAGCGCTCTTTGCGTGTCGGCAGCGGGATCTGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGTTGCTTgat                                                                            <  2:444683/140‑1 (MQ=255)                                                           gTACTGATCGCGCGCGTCTTTGTTGACGTGCGGGGAGATCAGAACAGTGAAGCGCTCTTTGCGTGTCGGCAGCGGGATCTGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGTTGCTTgat                                                                            <  2:982960/140‑1 (MQ=255)                                                            tACTGATCGCGCGCGTCTTTGTTGACGTGCGGGGAGATCAGAACAGTGAAGCGCTCTTTGCGTGTCGGCAGCGGGATCTGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGTTGCTTgatc                                                                           <  2:490828/140‑1 (MQ=255)                                                               tGATCGCGCGCGTCTTTGTTGACGTGCGGGGAGATCAGAACAGTGAAGCGCTCTTTGCGTGTCGGCAGCGGGATTTGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGTTGCTTgatcgat                                                                        <  2:190360/140‑1 (MQ=255)                                                               tGATCGCGCGCGTCTTTGTTGACGTGCGGGGAGATCAGAACAGTGAAGCGCTCTTTGCGTGTCGGCAGCGGGATCTGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGTTGCTTgatcgat                                                                        <  2:1054386/140‑1 (MQ=255)                                                               tGATCGCGCGCGTCTTTGTTGACGTGCGGGGAGATCAGAACAGTGAAGCGCTCTTTGCGTGTCGGCAGCGGGATCTGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGTTGCTTgatcgat                                                                        <  2:1242462/140‑1 (MQ=255)                                                               tGATCGCGCGCGTCTTTGTTGACGTGCGGGGAGATCAGAACAGTGAAGCGCTCTTTGCGTGTCGGCAGCGGGATCTGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGTTGCTTgatcgat                                                                        >  2:501449/1‑140 (MQ=255)                                                                gATCGCGCGCGTCTTTGTTGACGTGCGGGGAGATCAGAACAGTGAAGCGCTCTTTGCGTGTCGGCAGCGGGATCTGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGTTGCTTgatcgatc                                                                       >  1:111136/1‑140 (MQ=255)                                                                         cgTCTTTGTTGACGTGCGGGGAGATCAGAACAGTGAAGCGCTCTTTGCGTGTCGGCAGCGGGATCTGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGTTGCTTGATCGATCAGACgatga                                                              >  2:534011/1‑140 (MQ=255)                                                                           tCTTTGTTGACGTGCGGGGAGATCAGAACAGTGAAGCGCTCTTTGCGTGTCGGCAGCGGGATCTGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGTTGCTTGATCGATCAGACGATGATc                                                            >  1:1126780/1‑140 (MQ=255)                                                                           tCTTTGTTGACGTGCGGGGAGATCAGAACAGTGAAGCGCTCTTTGCGTGTCGGCAGCGGGATCTGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGTTGCTTGATCGATCAGACGATGATc                                                            >  1:866190/1‑140 (MQ=255)                                                                                    aCGTGCGGGGAGATCAGAACAGTGAAGCGCTCTTTGCGTGTCGGCAGCGGGATCTGACCACAGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGTTGCTTGATCGATCAGACGATGATCAAACGCttt                                                   >  2:792728/1‑140 (MQ=255)                                                                                      gTGCGGGGAGATCAGAACAGTGAAGCGCTCTTTGCGTGTCGGCAGCGGGATCTGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGTTGCTTGATCGATCAGACGATGATCAAACGCTTTCa                                                 >  2:521298/1‑140 (MQ=255)                                                                                           gggAGATCAGAACAGTGAAGCGCTCTTTGCGTGTCGGCAGCGGGATCTGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGTTGCTTGATCGATCAGACGATGATCAAACGCTTTCAGGCgg                                            >  2:282708/1‑140 (MQ=255)                                                                                              agaTCAGAACAGTGAAGCGCTCTTTGCGTGTCGGCAGCGGGATCTGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGTTGCTTGATCGATCAGACGATGATCAAACGCTTTCAGGCGGATa                                         >  2:1190532/1‑140 (MQ=255)                                                                                                aTCAGAACAGTGAAGCGCTCTTTGCGTGTCGGCAGCGGGATCTGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGTTGCTTGATCGATCAGACGATGATCAAACGCTTTCAGGCGGATACg                                       <  2:1080878/140‑1 (MQ=255)                                                                                                       cAGTGAAGCGCTCTTTGCGTGTCGGCAGCGGGATCTGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGTTGCTTGATCGATCAGACGATGATCAAACGCTTTCAGGCGGATACGGATTCtt                                <  2:111136/140‑1 (MQ=255)                                                                                                        aGTGAAGCGCTCTTTGCGTGTCGGCAGCGGGATCTGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGTTGCTTGATCGATCAGACGATGATCAAACGCTTTCAGGCGGATACGGATTCttt                               >  1:1136766/1‑140 (MQ=255)                                                                                                          tGAAGCGCTCTTTGCGTGTCGGCAGCGGGATCTGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGTTGCTTGATCGATCAGACGATGATCAAACGCTTTCAGGCGGATACGGATTCTTTgg                             >  2:916776/1‑140 (MQ=255)                                                                                                          tGAAGCGCTCTTTGCGTGTCGGCAGCGGGATCTGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGTTGCTTGATCGATCAGACGATGATCAAACGCTTTCAGGCGGATACGGATTCTTTgg                             >  1:1156846/1‑140 (MQ=255)                                                                                                          tGAAGCGCTCTTTGCGTGTCGGCAGCGGGATCTGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGTTGCTTGATCGATCAGACGATGATCAAACGCTTTCAGGCGGATACGGATTCTTTgg                             >  2:514896/1‑140 (MQ=255)                                                                                                          tGAAGCGCTCTTTGCGTGTCGGCAGCGGGATCTGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGTTGCTTGATCGATCAGACGATGATCAAACGCTTTCAGGCGGATACGGATTCTTTgg                             >  2:5585/1‑140 (MQ=255)                                                                                                          tGAAGCGCTCTTTGCGTGTCGGCAGCGGGATCTGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGTTGCTTGATCGATCAGACGATGATCAAACGCTTTCAGGCGGATACGGATTCTTTgg                             >  2:1223116/1‑140 (MQ=255)                                                                                                          tGAAGAGCTCTTTGCGTGTCGGCAGCGGGATCTGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGTTGCTTGATCGATCAGACGATGATCAAACGCTTTCAGGCGGATACGGATTCTTTgg                             <  2:1033276/140‑1 (MQ=255)                                                                                                            aaGCGCTCTTTGCGTGTCGGCAGCGGGATCTGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGTTGCTTGATCGATCAGACGATGATCAAACGCTTTCAGGCGGATACGGATTCTTTGGtt                           >  1:984651/1‑140 (MQ=255)                                                                                                            aaGCGCTCTTTGCGTGTCGGCAGCGGGATCTGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGTTGCTTGATCGATCAGACGATGATCAAACGCTTTCAGGCGGATACGGATTCTTTGGtt                           >  1:98532/1‑140 (MQ=255)                                                                                                            aaGCGCTCTTTGCGTGTCGGCAGCGGGATCTGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGTTGCTTGATCGATCAGACGATGATCAAACGCTTTCAGGCGGATACGGATTCTTTGGtt                           <  2:664688/140‑1 (MQ=255)                                                                                                             aGCGCTCTTTGCGTGTCGGCAGCGGGATCTGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGTTGCTTGATCGATCAGACGATGATCAAACGCTTTCAGGCGGATACGGATTCTTTGGTTc                          <  2:932858/140‑1 (MQ=255)                                                                                                             aGCGCTCTTTGCGTGTCGGCAGCGGGATCTGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGTTGCTTGATCGATCAGACGATGATCAAACGCTTTCAGGCGGATACGGATTCTTTGGTTc                          <  2:253624/140‑1 (MQ=255)                                                                                                             aGCGCTCTTTGCGTGTCGGCAGCGGGATCTGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGTTGCTTGATCGATCAGACGATGATCAAACGCTTTCAGGCGGATACGGATTCTTTGGTTc                          <  2:1001931/140‑1 (MQ=255)                                                                                                               cgcTCTTTGCGTGTCGGCAGCGGGATCTGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGTTGCTTGATCGATCAGACGATGATCAAACGCTTTCAGGCGGATACGGATTCTTTGGTTCTg                        <  2:641332/140‑1 (MQ=255)                                                                                                                 ctctTTGCGTGTCGGCAGCGGGATCTGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGTTGCTTGATCGATCAGACGATGATCAAACGCTTTCAGGCGGATACGGATTCTTTGGTTCTGCa                      <  2:921731/140‑1 (MQ=255)                                                                                                                 ctctTTGCGTGTCGGCAGCGGGATCTGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGTTGCTTGATCGATCAGACGATGATCAAACGCTTTCAGGCGGATACGGATTCTTTGGTTCTGCa                      >  2:769095/1‑140 (MQ=255)                                                                                                                  tctTTGCGTGTCGGCAGCGGGATCTGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGTTGCTTGATCGATCAGACGATGATCAAACGCTTTCAGGCGGATACGGATTCTTTGGTTCTGCAt                     <  2:598627/140‑1 (MQ=255)                                                                                                                       gCGTGTCGGCAGCGGGATCTGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGTTGCTTGATCGATCAGACGATGATCAAACGCTTTCAGGCGGATACGGATTCTTTGGTTCTGCATGAGAc                <  2:915755/140‑1 (MQ=255)                                                                                                                       gCGTGTCGGCAGCGGGATCTGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGTTGCTTGATCGATCAGACGATGATCAAACGCTTTCAGGCGGATACGGATTCTTTGGTTCTGCATGAGAc                <  1:593381/140‑1 (MQ=255)                                                                                                                         gtgtCGGCAGCGGGATCTGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGTTGCTTGATCGATCAGACGATGATCAAACGCTTTCAGGCGGATACGGATTCTTTGGTTCTGCATGAGACCa              <  2:98532/140‑1 (MQ=255)                                                                                                                            tCGGCAGCGGGATCTGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGTTGCTTGATCGATCAGACGATGATCAAACGCTTTCAGGCGGATACGGATTCTTTGGTTCTGCATGAGACCagag           >  1:1313225/1‑140 (MQ=255)                                                                                                                             cGGCAGCGGGATCTGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGTTGCTTGATCGATCAGACGATGATCAAACGCTTTCAGGCGGATACGGATTCTTTGGTTCTGCATGAGACCAGAGc          <  2:1029478/140‑1 (MQ=255)                                                                                                                                     ggATCTGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGTTGCTTGATCGATCAGACGATGATCAAACGCTTTCAGGCGGATACGGATTCTTTGGTTCTGCATGAGACCAGAGCTCCAatta  <  1:348536/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                       CAACGGTTTTCTCGGTTGGCTCAACGATGTCAACCAGACGCAAGTGAGTACGGATTTCGTACTGATCGCGCGCGTCTTTGTTGACGTGCGGGGAGATCAGAACAGTGAAGCGCTCTTTGCGTGTCGGCAGCGGGATCGGACCACGGACCTGCGCACCAGTGCGCTTGGCAGTCTCGACGATTTCCGCGGTTGCTTGATCGATCAGACGATGATCAAACGCTTTCAGGCGGATACGGATTCTTTGGTTCTGCATGAGACCAGAGCTCCAATTA  >  NC_000913/3453018‑3453289

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑