Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,196,475	C→A	E112* (GAA→TAA)	rsd ←	regulator of sigma D

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,196,475	0	C	A	100.0%	284.4 / NA	79	E112* (GAA→TAA)	rsd	regulator of sigma D
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (34/45);  total (34/45)

AGGATGTTTGACGCGGGCGGCGTCAAGCACCAGCAGAATCAGCTTATCTTCCAGCACAAAGCGCGCTTCCAGCGCCTCGCCGATGTCAGATAAAACCTGTTGAAACTCAAGGTAATTATCATGATCGATGGCGGTTTCCAGACTGGAATCGTAGTAATCCATAATCTGTTGAGTATTGGCTTCGAGTTGCGGCCAAATCTTCGCGGCGCGCGCGAGTTGCCCGTTGCCTTCCAGCTTATGAAGAATGCGCTCATAAATACTGAAATGTCCGG  >  NC_000913/4196338‑4196609                                                                                                                                          |                                                                                                                                       aGGATGTTTGACGCGGGCGGCGTCAAGCACCAGCAGAATCAGCTTATCTTCCAGCACAAAGCGCGCTTCCAGCGCCTCGCCGATGTCAGATAAAACCTGTTGAAACTCAAGGTAATTATCATGATCGATGGCGGTTTACa                                                                                                                                      <  1:1273435/140‑1 (MQ=255)  ggATGTTTGACGCGGGCGGCGTCAAGCACCAGCAGAATCAGCTTATCTTCCAGCACAAAGCGCGCTTCCAGCGCCTCGCCGATGTCAGATAAAACCTGTTGAAACTCAAGGTAATTATCATGATCGATGGCGGTTTACAg                                                                                                                                     >  1:933790/1‑140 (MQ=255)    aTGTTTGACGCGGGCGGCGTCAAGCACCAGCAGAATCAGCTTATCTTCCAGCACAAAGCGCGCTTCCAGCGCCTCGCCGATGTCAGATAAAACCTGTTGAAACTCAAGGTAATTATCATGATCGATGGCGGTTTACAGAc                                                                                                                                   >  1:1059620/1‑140 (MQ=255)    aTGTTTGACGCGGGCGGCGTCAAGCACCAGCAGAATCAGCTTATCTTCCAGCACAAAGCGCGCTTCCAGCGCCTCGCCGATGTCAGATAAAACCTGTTGAAACTCAAGGTAATTATCATGATCGATGGCGGTTTACAGAc                                                                                                                                   >  1:1091006/1‑140 (MQ=255)    aTGTTTGACGCGGGCGGCGTCAAGCACCAGCAGAATCAGCTTATCTTCCAGCACAAAGCGCGCTTCCAGCGCCTCGCCGATGTCAGATAAAACCTGTTGAAACTCAAGGTAATTATCATGATCGATGGCGGTTTACAGAc                                                                                                                                   <  2:113057/140‑1 (MQ=255)    aTGTTTGACGCGGGCGGCGTCAAGCACCAGCAGAATCAGCTTATCTTCCAGCACAAAGCGCGCTTCCAGCGCCTCGCCGATGTCAGATAAAACCTGTTGAAACTCAAGGTAATTATCATGATCGATGGCGGTTTACAGAc                                                                                                                                   <  1:1048912/140‑1 (MQ=255)    aTGTTTGACGCGGGCGGCGTCAAGCACCAGCAGAATCAGCTTATCTTCCAGCACAAAGCGCGCTTCCAGCGCCTCGCCGATGTCAGATAAAACCTGTTGAAACTCAAGGTAATTATCATGATCGATGGCGGTTTACAGAc                                                                                                                                   <  2:1059620/140‑1 (MQ=255)    aTGTTTGACGCGGGCGGCGTCAAGCACCAGCAGAATCAGCTTATCTTCCAGCACAAAGCGCGCTTCCAGCGCCTCGCCGATGTCAGATAAAACCTGTTGAAACTCAAGGTAATTATCATGATCGATGGCGGTTTACAGAc                                                                                                                                   <  1:987184/140‑1 (MQ=255)    aTGTTTGACGCGGGCGGCGTCAAGCACCAGCAGAATCAGCTTATCTTCCAGCACAAAGCGCGCTTCCAGCGCCTCGCCGATGTCAGATAAAACCTGTTGAAACTCAAGGTAATTATCATGATCGATGGCGGTTTACAGAc                                                                                                                                   <  2:756649/140‑1 (MQ=255)     tGTTTGACGCGGGCGGCGTCAAGCACCAGCAGAATCAGCTTATCTTCCAGCACAAAGCGCGCTTCCAGCGCCTCGCCGATGTCAGATAAAACCTGTTGAAACTCAAGGTAATTATCATGATCGATGGCGGTTTACAGACt                                                                                                                                  >  2:487787/1‑140 (MQ=255)         tGACGCGGGCGGCGTCAAGCACCAGCAGAATCAGCTTATCTTCCAGCACAAAGCGCGCTTCCAGCGCCTCGCCGATGTCAGATAAAACCTGTTGAAACTCAAGGTAATTATCATGATCGATGGCGGTTTACAGACTGGaa                                                                                                                              <  1:542999/140‑1 (MQ=255)         tGACGCGGGCGGCGTCAAGCACCAGCAGAATCAGCTTATCTTCCAGCACAAAGCGCGCTTCCAGCGCCTCGCCGATGTCAGATAAAACCTGTTGAAACTCAAGGTAATTATCATGATCGATGGCGGTTTACAGACTGGaa                                                                                                                              >  2:496434/1‑140 (MQ=255)          gACGCGGGCGGCGTCAAGCACCAGCAGAATCAGCTTATCTTCCAGCACAAAGCGCGCTTCCAGCGCCTCGCCGATGTCAGATAAAACCTGTTGAAACTCAAGGTAATTATCATGATCGATGGCGGTTTACAGACTGGAAt                                                                                                                             <  2:874142/140‑1 (MQ=255)              cgggcggCGTCAAGCACCAGCAGAATCAGCTTATCTTCCAGCACAAAGCACGCTTCCAGCGCCTCGCCGATGTCAGATAAAACCTGTTGAAACTCAAGGTAATTATCATGATCGATGGCGGTTTACAGACTGGAATCgta                                                                                                                         <  2:1235827/140‑1 (MQ=255)                ggcggcGTCAAGCACCAGCAGAATCAGCTTATCTTCCAGCACAAAGCGCGCTTCCAGCGCCTCGCCGATGTCAGATAAAACCTGTTGAAACTCAAGGTAATTATCATGATCGATGGCGGTTTACAGACTGGAATCgtagt                                                                                                                       >  2:1252646/1‑140 (MQ=255)                       tCAAGCACCAGCAGAATCAGCTTATCTTCCAGCACAAAGCGCGCTTCCAGCGCCTCGCCGATGTCAGATAAAACCTGTTGAAACTCAAGGTAATTATCATGATCGATGGCGGTTTACAGACTGGAATCGTAGTAATCCAt                                                                                                                <  1:1252646/140‑1 (MQ=255)                        cAAGCACCAGCAGAATCAGCTTATCTTCCAGCACAAAGCGCGCTTCCAGCGCGTCGCCGATGTCAGATAAAACCTGTTGAAACTCAAGGTAATTATCATGATCGATGGCGGTTTACAGACTGGAATCGTAGTAATCCATa                                                                                                               <  2:632826/140‑1 (MQ=255)                        cAAGCACCAGCAGAATCAGCTTATCTTCCAGCACAAAGCGCGCTTCCAGCGCCTCGCCGATGTCAGATAAAACCTGTTGAAACTCAAGGTAATTATCATGATCGATGGCGGTTTACAGACTGGAATCGTAGTAATCCATa                                                                                                               <  1:1140052/140‑1 (MQ=255)                        cAAGCACCAGCAGAATCAGCTTATCTTCCAGCACAAAGCGCGCTTCCAGCGCCTCGCCGATGTCAGATAAAACCTGTTGAAACTCAAGGTAATTATCATGATCGATGGCGGTTTACAGACTGGAATCGTAGTAATCCATa                                                                                                               <  2:1132826/140‑1 (MQ=255)                        cAAGCACCAGCAGAATCAGCTTATCTTCCAGCACAAAGCGCGCTTCCAGCGCCTCGCCGATGTCAGATAAAACCTGTTGAAACTCAAGGTAATTATCATGATCGATGGCGGTTTACAGACTGGAATCGTAGTAATCCATa                                                                                                               <  2:359345/140‑1 (MQ=255)                          aGCACCAGCAGAATCAGCTTATCTTCCAGCACAAAGCGCGCTTCCAGCGCCTCGCCGATGTCAGATAAAACCTGTTGAAACTCAAGGTAATTATCATGATCGATGGCGGTTTACAGACTGGAATCGTAGTAATCCATAAt                                                                                                             <  2:874501/140‑1 (MQ=255)                          aGCACCAGCAGAATCAGCTTATCTTCCAGCACAAAGCGCGCTTCCAGCGCCTCGCCGATGTCAGATAAAACCTGTTGAAACTCAAGGTAATTATCATGATCGATGGCGGTTTACAGACTGGAATCGTAGTAATCCATAAt                                                                                                             <  1:479731/140‑1 (MQ=255)                          aGCACCAGCAGAATCAGCTTATCTTCCAGCACAAAGCGCGCTTCCAGCGCCTCGCCGATGTCAGATAAAACCTGTTGAAACTCAAGGTAATTATCATGATCGATGGCGGTTTACAGACTGGAATCGTAGTAATCCATAAt                                                                                                             <  1:496434/140‑1 (MQ=255)                             aCCAGCAGAATCAGCTTATCTTCCAGCACAAAGCGCGCTTCCAGCGCCTCGCCGATGTCAGATAAAACCTGTTGAAACTCAAGGTAATTATCATGATCGATGGCGGTTTACAGACTGGAATCGTAGTAATCCATAATCTg                                                                                                          <  2:223390/140‑1 (MQ=255)                              ccAGCAGAATCAGCTTATCTTCCAGCACAAAGCGCGCTTCCAGCGCCTCGCCGATGTCAGATAAAACCTGTTGAAACTCAAGGTAATTATCATGATCGATGGCGGTTTACAGACTGGAATCGTAGTAATCCATAATCTGt                                                                                                         <  2:699874/140‑1 (MQ=255)                               cagcagAATCAGCTTATCTTCCAGCACAAAGCGCGCTTCCAGCGCCTCGCCGATGTCAGATAAAACCTGTTGAAACTCAAGGTAATTATCATGATCGATGGCGGTTTACAGACTGGAATCGTAGTAATCCATAATCTGtt                                                                                                        <  2:1033925/140‑1 (MQ=255)                                     aaTCAGCTTATCTTCCAGCACAAAGCGCGCTTCCAGCGCCTCGCCGATGTCAGATAAAACCTGTTGAAACTCAAGGTAATTATCATGATCGATGGCGGTTTACAGACTGGAATCGTAGTAATCCATAATCTGTTGAGTAt                                                                                                  >  2:427454/1‑140 (MQ=255)                                           cTTATCTTCCAGCACAAAGCGCGCTTCCAGCGCCTCGCCGATGTCAGATAAAACCTGTTGAAACTCAAGGTAATTATCATGATCGATGGCGGTTTACAGACTGGAATCGTAGTAATCCATAATCTGTTGAGTATTGGCtt                                                                                            >  1:798815/1‑140 (MQ=255)                                           cTTATCTTCCAGCACAAAGCGCGCTTCCAGCGCCTCGCCGATGTCAGATAAAACCTGTTGAAACTCAAGGTAATTATCATGATCGATGGCGGTTTACAGACTGGAATCGTAGTAATCCATAATCTGTTGAGTATTGGCtt                                                                                            <  2:254313/140‑1 (MQ=255)                                           cTTATCTTCCAGCACAAAGCGCGCTTCCAGCGCCTCGCCGATGTCAGATAAAACCTGTTGAAACTCAAGGTAATTATCATGATCGATGGCGGTTTACAGACTGGAATCGTAGTAATCCATAATCTGTTGAGTATTGGCtt                                                                                            >  1:882134/1‑140 (MQ=255)                                               tCTTCCAGCACAAAGCGCGCTTCCAGCGCCTCGCCGATGTCAGATAAAACCTGTTGAAACTCAAGGTAATTATCATGATCGATGGCGGTTTACAGACTGGAATCGTAGTAATCCATAATCTGTTGAGTATTGGCTTCGAg                                                                                        >  1:739366/1‑140 (MQ=255)                                                 ttCCAGCACAAAGCGCGCTTCCAGCGCCTCGCCGATGTCAGATAAAACCTGTTGAAACTCAAGGTAATTATCATGATCGATGGCGGTTTACAGACTGGAATCGTAGTAATCCATAATCTGTTGAGTATTGGCTTCGAGtt                                                                                      <  1:774489/140‑1 (MQ=255)                                                     aGCACAAAGCGCGCTTCCAGCGCCTCGCCGATGTCAGATAAAACCTGTTGAAACTCAAGGTAATTATCATGATCGATGGCGGTTTACAGACTGGAATCGTAGTAATCCATAATCTGTTGAGTATTGGCTTCGAGTTGCgg                                                                                  >  2:431441/1‑140 (MQ=255)                                                            aGCGCGCTTCCAGCGCCTCGCCGATGTCAGATAAAACCTGTTGAAACTCAAGGTAATTATCATGATCGATGGTGGTTTACAGACTGGAATCGTAGTAATCCATAATCTGTTGAGTATTGGCTTCGAGTTGCGGCCAAATc                                                                           >  1:269825/1‑140 (MQ=255)                                                            aGCGCGCTTCCAGCGCCTCGCCGATGTCAGATAAAACCTGTTGAAACTCAAGGTAATTATCATGATCGATGGCGGTTTACAGACTGGAATCGTAGTAATCCATAATCTGTTGAGTATTGGCTTCGAGTTGCGGCCAAATc                                                                           >  2:237756/1‑140 (MQ=255)                                                                 gcTTCCAGCGCCTCGCCGATGTCAGATAAAACCTGTTGAAACTCAAGGTAATTATCATGATCGATGGCGGTTTACAGACTGGAATCGTAGTAATCCATAATCTGTTGAGTATTGGCTTCGAGTTGCGGCCAAATCTTcgc                                                                      >  2:1226563/1‑140 (MQ=255)                                                                               gCCGATGTCAGATAAAACCTGTTGAAACTCAAGGTAATTATCATGATCGATGGCGGTTTACAGACTGGAATCGTAGTAATCCATAATCTGTTGAGTATTGGCTTCGAGTTGCGGCCAAATCTTCGCGGCGCGCGCGAGtt                                                        >  2:1102886/1‑140 (MQ=255)                                                                               gCCGATGTCAGATAAAACCTGTTGAAACTCAAGGTAATTATCATGATCGATGGCGG‑TTACAGACTGGAATCGTAGTAATCCATAATCTGTTGAGTATTGGCTTCGAGTTGCGGCCAAATCTTCGCGGCGCGCGCGAGTTg                                                       >  1:1253741/1‑140 (MQ=255)                                                                                     gTCAGATAAAACCTGTTGAAACTCAAGGTAATTATCATGATCGATGGCGGTTTACAGACTGGAATCGTAGTAATCCATAATCTGTTGAGTATTGGCTTCGAGTTGCGGCCAAATCTTCGCGGCGCGCGCGAGTTGCCCGt                                                  <  2:1196234/140‑1 (MQ=255)                                                                                     gTCAGATAAAACCTGTTGAAACTCAAGGTAATTATCATGATCGATGGCGGTTTACAGACTGGAATCGTAGTAATCCATAATCTGTTGAGTATTGGCTTCGAGTTGCGGCCAAATCTTCGCGGCGCGCGCGAGTTGCCCGt                                                  <  1:39050/140‑1 (MQ=255)                                                                                      tCAGATAAAACCTGTTGAAACTCAAGGTAATTATCATGATCGATGGCGGTTTACAGACTGGAATCGTAGTAATCCATAATCTGTTGAGTATTGGCTTCGAGTTGCGGCCAAATCTTCGCGGCGCGCGCGAGTTGCCCGtt                                                 <  1:856432/140‑1 (MQ=255)                                                                                           tAAAACCTGTTGAAACTCAAGGTAATTATCATGATCGATGGCGGTTTACAGACTGGAATCGTAGTAATCCATAATCTGTTGAGTATTGGCTTCGAGTTGCGGCCAAATCTTCGCGGCGCGCGCGAGTTGCCCGTTGCCtt                                            <  2:22249/140‑1 (MQ=255)                                                                                             aaaCCTGTTGAAACTCAAGGTAATTATCATGATCGATGGCGGTTTACAGACTGGAATCGTAGTAATCCATAATCTGTTGAGTATTGGCTTCGAGTTGCGGCCAAATCTTCGCGGCGCGCGCGAGTTGCCCGTTGCCTTcc                                          <  2:94698/140‑1 (MQ=255)                                                                                             aaaCCTGTTGAAACTCAAGGTAATTATCATGATCGATGGCGGTTTACAGACTGGAATCGTAGTAATCCATAATCTGTTGAGTATTGGCTTCGAGTTGCGGCCAAATCTTCGCGGCGCGCGCGAGTTGCCCGTTGCCTTcc                                          <  2:757149/140‑1 (MQ=255)                                                                                               aCCTGTTGAAACTCAAGGTAATTATCATGATCGATGGCGGTTTACAGACTGGAATCGTAGTAATCCATAATCTGTTGAGTATTGGCTTCGAGTTGCGGCCAAATCTTCGCGGCGCGCGCGAGTTGCCCGTTGCCTTCCAg                                        <  2:293753/140‑1 (MQ=255)                                                                                                ccTGTTGAAACTCAAGGTAATTATCATGATCGATGGCGGTTTACAGACTGGAATCGTAGTAATCCATAATCTGTTGAGTATTGGCTTCGAGTTGCGGCCAAATCTTCGCGGCGCGCGCGAGTTGCCCGTTGCCTTCCAGc                                       >  1:849249/1‑140 (MQ=255)                                                                                                 cTGTTGAAACTCAAGGTAATTATCATGATCGATGGCGGTTTACAGACTGGAATCGTAGTAATCCATAATCTGTTGAGTATTGGCTTCGAGTTGCGGCCAAATCTTCGCGGCGCGCGCGAGTTGCCCGTTGCCTTCCAGCt                                      <  1:431441/140‑1 (MQ=255)                                                                                                 cTGTTGAAACTCAAGGTAATTATCATGATCGATGGCGGTTTACAGACTGGAATCGTAGTAATCCATAATCTGTTGAGTATTGGCTTCGAGTTGCGGCCAAATCTTCGCGGCGCGCGCGAGTTGCCCGTTGCCTTCCAGCt                                      <  2:1091006/140‑1 (MQ=255)                                                                                                  tGTTGAAACTCAAGGTAATTATCATGATCGATGGCGGTTTACAGACTGGAATCGTAGTAATCCATAATCTGTTGAGTATTGGCTTCGAGTTGCGGCCAAATCTTCGCGGCGCGCGCGAGTTGCCCGTTGCCTTcc                                          <  2:495719/135‑1 (MQ=255)                                                                                                  tGTTGAAACTCAAGGTAATTATCATGATCGATGGCGGTTTACAGACTGGAATCGTAGTAATCCATAATCTGTTGAGTATTGGCTTCGAGTTGCGGCCAAATCTTCGCGGCGCGCGCGAGTTGCCCGTTGCCTTcc                                          >  1:495719/1‑135 (MQ=255)                                                                                                    ttGAAACTCAAGGTAATTATCATGATCGATGGCGGTTTACAGACTGGAATCGTAGTAATCCATAATCTGTTGAGTATTGGCTTCGAGTTGCGGCCAAATCTTCGCGGCGCGCGCGAGTTGCCCGTTGCCTTcc                                          >  1:41185/1‑133 (MQ=255)                                                                                                    ttGAAACTCAAGGTAATTATCATGATCGATGGCGGTTTACAGACTGGAATCGTAGTAATCCATAATCTGTTGAGTATTGGCTTCGAGTTGCGGCCAAATCTTCGCGGCGCGCGCGAGTTGCCCGTTGCCTTcc                                          <  2:41185/133‑1 (MQ=255)                                                                                                    ttGAAACTCAAGGTAATTATCATGATCGATGGCGGTTTACAGACTGGAATCGTAGTAATCCATAATCTGTTGAGTATTGGCTTCGAGTTGCGGCCAAATCTTCGCGGCGCGCGCGAGTTGCCCGTTGCCTTCCAGCTTAt                                   >  2:277890/1‑140 (MQ=255)                                                                                                    ttGAAACTCAAGGTAATTATCATGATCGATGGCGGTTTACAGACTGGAATCGTAGTAATCCATAATCTGTTGAGTATTGGCTTCGAGTTACGGCCAAATCTTCGCGGCGCGCGCGAGTTGCCCGTTGCCTTCCAGCTTAt                                   <  2:170792/140‑1 (MQ=255)                                                                                                            cAAGGTAATTATCATGATCGATGGCGGTTTACAGACTGGAATCGTAGTAATCCATAATCTGTTGAGTATTGGCTTCGAGTTGCGGCCAAATCTTCGCGGCGCGCGCGAGTTGCCCGTTGCCTTCCAGCTTATGAAGAATg                           <  1:487787/140‑1 (MQ=255)                                                                                                            cAAGGTAATTATCATGATCGATGGCGGTTTACAGACTGGAATCGTAGTAATCCATAATCTGTTGAGTATTGGCTTCGAGTTGCGGCCAAATCTTCGCGGCGCGCGCGAGTTGCCCGTTGCCTTCCAGCTTATGAAGAATg                           <  1:52486/140‑1 (MQ=255)                                                                                                            cAAGGTAATTATCATGATCGATGGCGGTTTACAGACTGGAATCGTAGTAATCCATAATCTGTTGAGTATTGGCTTCGAGTTGCGGCCAAATCTTCGCGGCGCGCGCGAGTTGCCCGTTGCCTTCCAGCTTATGAAGAATg                           <  2:1074569/140‑1 (MQ=255)                                                                                                              aGGTAATTATCATGATCGATGGCGGTTTACAGACTGGAATCGTAGTAATCCATAATCTGTTGAGTATTGGCTTCGAGTTGCGGCCAAATCTTCGCGGCGCGCGCGAGTTGCCCGTTGCCTTCCAGCTTATGAAGAATgcg                         >  1:1149601/1‑140 (MQ=255)                                                                                                              aGGTAATTATCATGATCGATGGCGGTTTACAGACTGGAATCGTAGTAATCCATAATCTGTTGAGTATTGGCTTCGAGTTGCGGCCAAATCTTCGCGGCGCGCGCGAGTTGCCCGTTGCCTTCCAGCTTATGAAGAATgcg                         <  2:595977/140‑1 (MQ=255)                                                                                                              aGGTAATTATCATGATCGATGGCGGTTTACAGACTGGAATCGTAGTAATCCATAATCTGTTGAGTATTGGCTTCGAGTTGCGGCCAAATCTTCGCGGCGCGCGCGAGTTGCCCGTTGCCTTCCAGCTTATGAAGAATgcg                         <  1:427454/140‑1 (MQ=255)                                                                                                              aGGTAATTATCATGATCGATGGCGGTTTACAGACTGGAATCGTAGTAATCCATAATCTGTTGAGTATTGGCTTCGAGTTGCGGCCAAATCTTCGCGGCGCGCGCGAGTTGCCCGTTGCCTTCCAGCTTATGAAGAATgcg                         <  1:533706/140‑1 (MQ=255)                                                                                                              aGGTAATTATCATGATCGATGGCGGTTTACAGACTGGAATCGTAGTAATCCATAATCTGTTGAGTATTGGCTTCGAGTTGCGGCCAAATCTTCGCGGCGCGCGCGAGTTGCCCGTTGCCTTCCAGCTTATGAAGAATgcg                         <  2:1234182/140‑1 (MQ=255)                                                                                                                   aTTATCATGATCGATGGTGGTTTACAGACTGGAATCGTAGTAATCCATAATCTGTTGAGTATTGGCTTCGAGTTGCGGCCAAATCTTCGCGGCGCGCGCGAGTTGCCCGTTGCCTTCCAGCTTATGAAGAATGCGCTCAt                    <  2:269825/140‑1 (MQ=255)                                                                                                                        cATGATCGATGGCGGTTTACAGACTGGAATCGTAGTAATCCATAATCTGTTGAGTATTGGCTTCGAGTTGCGGCCAAATCTTCGCGGCGCGCGCGAGTTGCCCGTTGCCTTCCAGCTTATGAAGAATGCGCTCATAAATa               <  2:1156795/140‑1 (MQ=255)                                                                                                                         aTGATCGATGGCGGTTTACAGACTGGAATCGTAGTAATCCATAATCTGTTGAGTATTGGCTTCGAGTTGCGGCCAAATCTTCGCGGCGCGCGCGAGTTGCCCGTTGCCTTCCAGCTTATGAAGAATGCGCTCATAAATAc              >  2:536634/1‑140 (MQ=255)                                                                                                                          tGATCGATGGCGGTTTACAGACTGGAATCGTAGTAATCCATAATCTGTTGAGTATTGGCTTCGAGTTGCGGCCAAATCTTCGCGGCGCGCGCGAGTTGCCCGTTGCCTTCCAGCTTATGAAGAATGCGCTCATAAATACt             >  2:588145/1‑140 (MQ=255)                                                                                                                          tGATCGATGGCGGTTTACAGACTGGAATCGTAGTAATCCATAATCTGTTGAGTATTGGCTTCGAGTTGCGGCCAAATCTTCGCGGCGCGCGCGAGTTGCCCGTTGCCTTCCAGCTTATGAAGAATGCGCTCATAAATACt             >  2:1154531/1‑140 (MQ=255)                                                                                                                           gATCGATGGCGGTTTACAGACTGGAATCGTAGTAATCCATAATCTGTTGAGTATTGGCTTCGAGTTGCGGCCAAATCTTCGCGGCGCGCGCGAGTTGCCCGTTGCCTTCCAGCTTATGAAGAATGCGCTCATAAATACTg            >  2:232750/1‑140 (MQ=255)                                                                                                                           gATCGATGGCGGTTTACAGACTGGAATCGTAGTAATCCATAATCTGTTGAGTATTGGCTTCGAGTTGCGGCCAAATCTTCGCGGCGCGCGCGAGTTGCCCGTTGCCTTCCAGCTTATGAAGAATGCGCTCATAAATACTg            >  2:671408/1‑140 (MQ=255)                                                                                                                           gATCGATGGCGGTTTACAGACTGGAATCGTAGTAATCCATAATCTGTTGAGTATTGGCTTCGAGTTGCGGCCAAATCTTCGCGGCGCGCGCGAGTTGCCCGTTGCCTTCCAGCTTATGAAGAATGCGCTCATAAATACTg            >  1:312614/1‑140 (MQ=255)                                                                                                                            aTCGATGGCGGTTTACAGACTGGAATCGTAGTAATCCATAATCTGTTGAGTATTGGCTTCGAGTTGCGGCCAAATCTTCGCGGCGCGCGCGAGTTGCCCGTTGCCTTCCAGCTTATGAAGAATGCGCTCATAAATACTGa           >  2:458380/1‑140 (MQ=255)                                                                                                                             tCGATGGCGGTTTACAGACTGGAATCGTAGTAATCCATAATCTGTTGAGTATTGGCTTCGAGTTGCGGCCAAATCTTCGCGGCGCGCGCGAGTTGCCCGTTGCCTTCCAGCTTATGAAGAATGCGCTCATAAATACTGaa          >  2:418356/1‑140 (MQ=255)                                                                                                                             tCGATGGCGGTTTACAGACTGGAATCGTAGTAATCCATAATCTGTTGAGTATTGGCTTCGAGTTGCGGCCAAATCTTCGCGGCGCGCGCGAGTTGCCCGTTGCCTTCCAGCTTATGAAGAATGCGCTCATAAATACTGaa          >  2:1265173/1‑140 (MQ=255)                                                                                                                             tCGATGGCGGTTTACAGACTGGAATCGTAGTAATCCATAATCTGTTGAGTATTGGCTTCGAGTTGCGGCCAAATCTTCGCGGCGCGCGCGAGTTGCCCGTTGCCTTCCAGCTTATGAAGAATGCGCTCATAAATACTGaa          >  2:695988/1‑140 (MQ=255)                                                                                                                               gATGGCGGTTTACAGACTGGAATCGTAGTAATCCATAATCTGTTGAGTATTGGCTTCGAGTTGCGGCCAAATCTTCGCGGCGCGCGCGAGTTGCCCGTTGCCTTCCAGCTTATGAAGAATGCGCTCATAAATACTGAAAt        <  1:232750/140‑1 (MQ=255)                                                                                                                                aTGGCGGTTTACAGACTGGAATCGTAGTAATCCATAATCTGTTGAGTATTGGCTTCGAGTTGCGGCCAAATCTTCGCGGCGCGCGCGAGTTGCCCGTTGCCTTCCAGCTTATGAAGAATGCGCTCATAAATACTGAAATg       >  1:807676/1‑140 (MQ=255)                                                                                                                                 tGGCGGTTTACAGACTGGAATCGTAGTAATCCATAATCTGTTGAGTATTGGCTTCGAGTTGCGGCCAAATCTTCGCGGCGCGCGCGAGTTGCCCGTTGCCTTCCAGCTTATGAAGAATGCGCTCATAAATACTGAAATGt      >  1:852363/1‑140 (MQ=255)                                                                                                                                 tGGCGGTTTACAGACTGGAATCGTAGTAATCCATAATCTGTTGAGTATTGGCTTCGAGTTGCGGCCAAATCTTCGCGGCGCGCGCGAGTTGCCCGTTGCCTTCCAGCTTATGAAGAATGCGCTCATAAATACTGAAATGt      >  2:893123/1‑140 (MQ=255)                                                                                                                                  ggCGGTTTACAGACTGGAATCGTAGTAATCCATAATCTGTTGAGTATTGGCTTCGAGTTGCGGCCAAATCTTCGCGGCGCGCGCGAGTTGCCCGTTGCCTTCCAGCTTATGAAGAATGCGCTCATAAATACTGAAATGTc     >  2:1223696/1‑140 (MQ=255)                                                                                                                                     ggTTTACAGACTGGAATCGTAGTAATCCATAATCTGTTGAGTATTGGCTTCGAGTTGCGGCCAAATCTTCGCGGCGCGCGCGAGTTGCCCGTTGCCTTCCAGCTTATGAAGAATGCGCTCATAAATACTGAAATGTCCgg  <  1:1226563/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                       AGGATGTTTGACGCGGGCGGCGTCAAGCACCAGCAGAATCAGCTTATCTTCCAGCACAAAGCGCGCTTCCAGCGCCTCGCCGATGTCAGATAAAACCTGTTGAAACTCAAGGTAATTATCATGATCGATGGCGGTTTCCAGACTGGAATCGTAGTAATCCATAATCTGTTGAGTATTGGCTTCGAGTTGCGGCCAAATCTTCGCGGCGCGCGCGAGTTGCCCGTTGCCTTCCAGCTTATGAAGAATGCGCTCATAAATACTGAAATGTCCGG  >  NC_000913/4196338‑4196609

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑