Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,354,821	G→T	A217E (GCG→GAG)	sapB ←	putrescine ABC exporter membrane subunit SapB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,354,821	0	G	T	100.0%	343.9 / NA	95	A217E (GCG→GAG)	sapB	putrescine ABC exporter membrane subunit SapB
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (38/57);  total (38/57)

AACCATTTCGGTGATCATCGCCAGCGTCAGCATGGTGGAAAACTGTAAGCCCAGGCGAGGAATAACCGGCGGTAACGCGTTATGCAGAACGTGGCGACGCAAAATAGTAAAGCGCGACAAACCGCGGGTCGCCGCCGCTTTTACATAGTTCTGGTCATACACTTCGATAGTGCTGATACGCATCAGGCGGATCACTTCAGTTGTTGGCGCAACCGACAGGGTGATCACGGGCAATATCATATGGCGGATTGCGCTCATGAT  >  NC_000913/1354685‑1354945                                                                                                                                         |                                                                                                                             aaCCATTTCGGTGATCATCGCCAGCGTCAGCATGGTGGAAAACTGTAAGCCCAGGCGAGGAATAACCGGCGGTAACGCGTTATGCAGCACGTGGCGACGCAAAATAGTAAAGCGCGACAAACCGCGGGTCGCCGCCTCtt                                                                                                                           <  2:1175784/140‑1 (MQ=255) aaCCATTTCGGTGATCATCGCCAGCGTCAGCATGGTGGAAAACTGTAAGCCCAGGCGAGGAATAACCGGCGGTAACGCGTTATGCAGAACGTGGCGACGCAAAATAGTAAAGCGCGACAAACCGCGGGTCGCCGCCTCtt                                                                                                                           <  2:53673/140‑1 (MQ=255) aaCCATTTCGGTGATCATCGCCAGCGTCAGCATGGTGGAAAACTGTAAGCCCAGGCGAGGAATAACCGGCGGTAACGCGTTATGCAGAACGTGGCGACGCAAAATAGTAAAGCGCGACAAACCGCGGGTCGCCGCCTCtt                                                                                                                           <  2:350074/140‑1 (MQ=255)      tttCGGTGATCATCGCCAGCGTCAGCATGGTGGAAAACTGTAAGCCCAGGCGAGGAATAACCGGCGGTAACGCGTTATGCAGAACGTGGCGACGCAAAATAGTAAAGCGCGACAAACCGCGGGTCGCCGCCTCTTTTACa                                                                                                                      >  1:1105930/1‑140 (MQ=255)      tttCGGTGATCATCGCCAGCGTCAGCATGGTGGAAAACTGTAAGCCCAGGCGAGGAATAACCGGCGGTAACGCGTTATGCAGAACGTGGCGACGCAAAATAGTAAAGCGCGACAAACCGCGGGTCGCCGCCTCTTTTACa                                                                                                                      <  2:993209/140‑1 (MQ=255)      tttCGGTGATCATCGCCAGCGTCAGCATGGTGGAAAACTGTAAGCCCAGGCGAGGAATAACCGGCGGTAACGCGTTATGCAGAACGTGGCGACGCAAAATAGTAAAGCGCGACAAACCGCGGGTCGCCGCCTCTTTTACa                                                                                                                      >  2:142290/1‑140 (MQ=255)      tttCGGTGATCATCGCCAGCGTCAGCATGGTGGAAAACTGTAAGCCCAGGCGAGGAATAACCGGCGGTAACGCGTTATGCAGAACGTGGCGACGCAAAATAGTAAAGCGCGACAAACCGCGGGTCGCCGCCTCTTTTACa                                                                                                                      <  2:1182133/140‑1 (MQ=255)      tttCGGTGATCATCGCCAGCGTCAGCATGGTGGAAAACTGTAAGCCCAGGCGAGGAATAACCGGCGGTAACGCGTTATGCAGAACGTGGCGACGCAAAATAGTAAAGCGCGACAAACCGCGGGTCGCCGCCTCTTTTACa                                                                                                                      <  2:1084163/140‑1 (MQ=255)      tttCGGTGATCATCGCCAGCGTCAGCATGGTGGAAAACTGTAAGCCCAGGCGAGGAATAACCGGCGGTAACGCGTTATGCAGAACGTGGCGACGCAAAATAGTAAAGCGCGACAAACCGCGGGTCGCCGCCTCTTTTACa                                                                                                                      >  1:972969/1‑140 (MQ=255)      tttCGGTGATCATCGCCAGCGTCAGCATGGTGGAAAACTGTAAGCCCAGGCGAGGAATAACCGGCGGTAACGCGTTATGCAGAACGTGGCGACGCAAAATAGTAAAGCGCGACAAACCGCGGGTCGCCGCCTCTTTTACa                                                                                                                      <  1:548211/140‑1 (MQ=255)      tttCGGTGATCATCGCCAGCGTCAGCATGGTGGAAAACTGTAAGCCCAGGCGAGGAATAACCGGCGGTAACGCGTTATGCAGAACGTGGCGACGCAAAATAGTAAAGCGCGACAAACCGCGGGTCGCCGCCTCTTTTACa                                                                                                                      >  1:117444/1‑140 (MQ=255)      tttCGGTGATCATCGCCAGCGTCAGCATGGTGGAAAACTGTAAGCCCAGGCGAGGAATAACCGGCGGTAACGCGTTATGCAGAACGTGGCGACGCAAAATAGTAAAGCGCGACAAACCGCGGGTCGCCGCCTCTTTTACa                                                                                                                      <  1:111233/140‑1 (MQ=255)         cGGTGATCATCGCCAGCGTCAGCATGGTGGAAAACTGTAAGCCCAGGCGAGGAATAACCGGCGGTAACGCGTTATGCAGAACGTGGCGACGCAAAATAGTAAAGCGCGACAAACCGCGGGTCGCCGCCTCTTTTACATAg                                                                                                                   >  1:604001/1‑140 (MQ=255)          ggTGATCATCGCCAGCGTCAGCATGGTGGAAAACTGTAAGCCCAGGCGAGGAATAACCGGCGGTAACGCGTTATGCAGAACGTGGCGACGCAAAATAGTAAAGCGCGACAAACCGCGGGTCGCCGCCTCTTTTACATAGt                                                                                                                  <  2:836755/140‑1 (MQ=255)           gTGATCATCGCCAGCGTCAGCATGGTGGAAAACTGTAAGCCCAGGCGAGGAATAACCGGCGGTAACGCGTTATGCAGAACGTGGCGACGCAAAATAGTAAAGCGCGACAAACCGCGGGTCGCCGCCTCTTTTACATAGtt                                                                                                                 >  2:1147709/1‑140 (MQ=255)            tGATCATCGCCAGCGTCAGCATGGTGGAAAACTGTAAGCCCAGGCGAGGAATAACCGGCGGTAACGCGTTATGCAGAACGTGGCGACGCAAAATAGTAAAGCGCGACAAACCGCGGGTCGCCGCCTCTTTTACATAGTTc                                                                                                                <  1:878486/140‑1 (MQ=255)            tGATCATCGCCAGCGTCAGCATGGTGGAAAACTGTAAGCCCAGGCGAGGAATAACCGGCGGTAACGCGTTATGCAGAACGTGGCGACGCAAAATAGTAAAGCGCGACAAACCGCGGGTCGCCGCCTCTTTTACATAGTTc                                                                                                                <  2:255560/140‑1 (MQ=255)              atcatcGCCAGCGTCAGCATGGTGGAAAACTGTAAGCCCAGGCGAGGAATAACCGGCGGTAACGCGTTATGCAGAACGTGGCGACGCAAAATAGTAAAGCGCGACAAACCGCGGGTCGCCGCCTCTTTTACATAGTTCTg                                                                                                              >  2:195511/1‑140 (MQ=255)              atcatcGCCAGCGTCAGCATGGTGGAAAACTGTAAGCCCAGGCGAGGAATAACCGGCGGTAACGCGTTATGCAGAACGTGGCGACGCAAAATAGTAAAGCGCGACAAACCGCGGGTCGCCGCCTCTTTTACATAGTTCTg                                                                                                              >  1:86222/1‑140 (MQ=255)              atcatcGCCAGCGTCAGCATGGTGGAAAACTGTAAGCCCAGGCGAGGAATAACCGGCGGTAACGCGTTATGCAGAACGTGGCGACGCAAAATAGTAAAGCGCGACAAACCGCGGGTCGCCGCCTCTTTTACATAGTTCTg                                                                                                              >  1:494863/1‑140 (MQ=255)                  tcGCCAGCGTCAGCATGGTGGAAAACTGTAAGCCCAGGCGAGGAATAACCGGCGGTAACGCGTTATGCAGAACGTGGCGACGCAAAATAGTAAAGCGCGACAAACCGCGGGTCGCCGCCTCTTTTACATAGTTCTGGTCa                                                                                                          <  2:542003/140‑1 (MQ=255)                    gCCAGCGTCGGCATGGTGGAAACCTGTAAGCCCAGGCGAGGAATAACCGGCGGTAACGCGTTATGCAGAACGTGGCGACGCAAAATAGTAAATCGCGACAAACCGCGGGTCGCCGCCTCTTTTACATAGTTCTGGTCATa                                                                                                        <  2:1230954/140‑1 (MQ=255)                    gCCAGCGTCAGCATGGTGGAAAACTGTAAGCCCAGGCGAGGAATAACCGGCGGTAACGCGTTATGCAGAACGTGGCGACGCAAAATAGTAAAGCGCGACAAACCGCGGGTCGCCGCCTCTTTTACATAGTTCTGGTCATa                                                                                                        <  2:939276/140‑1 (MQ=255)                    gCCAGCGTCAGCATGGTGGAAAACTGTAAGCCCAGGCGAGGAATAACCGGCGGTAACGCGTTATGCAGAACGTGGCGACGCAAAATAGTAAAGCGCGACAAACCGCGGGTCGCCGCCTCTTTTACATAGTTCTGGTCATa                                                                                                        <  2:53262/140‑1 (MQ=255)                    gCCAGCGTCAGCATGGTGGAAAACTGTAAGCCCAGGCGAGGAATAACCGGCGGTAACGCGTTATGCAGAACGTGGCGACGCAAAATAGTAAAGCGCGACAAACCGCGGGTCGCCGCCTCTTTTACATAGTTCTGGTCATa                                                                                                        <  1:1155196/140‑1 (MQ=255)                       tgCGTCAGCATGGTGGAAAACTGTATGACCAGGCGAGGAATACCCGGCGGTAAGGCGTTTTGCAGATCGTGGCGACGCAAAATAGTAAGGCGCGACAACCCGCGGGTCGACGCCTCTTTTACATAGTTCTGGTCATactc                                                                                                     <  2:791770/139‑3 (MQ=255)                        gCGTCAGCATGGTGGAAAACTGTAAGCCCAGGCGAGGAATAACCGGCGGTAACGCGTTATGCAGAACGTGGCGACGCAAAATAGTAAAGCGCGACAAACCGCGGGTCGCCGCCTCTTTTACATAGtt                                                                                                                 >  2:900710/1‑127 (MQ=255)                        gCGTCAGCATGGTGGAAAACTGTAAGCCCAGGCGAGGAATAACCGGCGGTAACGCGTTATGCAGAACGTGGCGACGCAAAATAGTAAAGCGCGACAAACCGCGGGTCGCCGCCTCTTTTACATAGtt                                                                                                                 <  1:900710/127‑1 (MQ=255)                         cGTCAGCATGGTGGAAAACTGTAAGCCCAGGCGAGGAATAACCGGCGGTAACGCGTTATGCAGAACGTGGCGACGCAAAATAGTAAAGCGCGACAAACCGCGGGTCGCCGCCTCTTTTACATAGTTCTGGTCATACACtt                                                                                                   >  2:407720/1‑140 (MQ=255)                         cGTCAGCATGGTGGAAAACTGTAAGCCCAGGCGAGGAATAACCGGCGGTAACGCGTTATGCAGAACGTGGCGACGCAAAATAGTAAAGCGCGACAAACCGCGGGTCGCCGCCTCTTTTACATAGTTCTGGTCATACACtt                                                                                                   >  2:45477/1‑140 (MQ=255)                            cAGCATGGTGGAAAACTGTAAGCCCAGGCGAGGAATAACCGGCGGTAACGCGTTATGCAGAACGTGGCGACGCAAAATAGTAAAGCGCGACAAACCGCGGGTCGCCGCCTCTTTTACATAGTTCTGGTCATACACTTCGa                                                                                                <  2:1232047/140‑1 (MQ=255)                            cAGCATGGTGGAAAACTGTAAGCCCAGGCGAGGAATAACCGGCGGTAACGCGTTATGCAGAACGTGGCGACGCAAAATAGTAAAGCGCGACAAACCGCGGGTCGCCGCCTCTTTTACATAGTTCTGGTCATACACTTCGa                                                                                                <  1:719987/140‑1 (MQ=255)                            cAGCATGGTGGAAAACTGTAAGCCCAGGCGAGGAATAACCGGCGGTAACGCGTTATGCAGAACGTGGCGACGCAAAATAGTAAAGCGCGACAAACCGCGGGTCGCCGCCTCTTTTACATAGTTCTGGTCATACACTTCGa                                                                                                <  2:1682/140‑1 (MQ=255)                            cAGCATGGTGGAAAACTGTAAGCCCAGGCGAGGAATAACCGGCGGTAACGCGTTATGCAGAACGTGGCGACGCAAAATAGTAAAGCGCGACAAACCGCGGGTCGCCGCCTCTTTTACATAGTTCTGGTCATACACTTCGa                                                                                                <  1:45477/140‑1 (MQ=255)                             aGCATGGTGGAAAACTGTAAGCCCAGGCGAGGAATAACCGGCGGTAACGCGTTATGCAGAACGTGGCGACGCAAAATAGTAAAGCGCGACAAACCGCGGGTCGCCGCCTCTTTTACATAGTTCTGGTCATACACTTCGAt                                                                                               >  1:604532/1‑140 (MQ=255)                                 tggtggAAAACTGTAAGCCCAGGCGAGGAATAACCGGCGGTAACGCGTTATGCAGAACGTGGCGACGCAAAATAGTAAAGCGCGACAAACCGCGGGTCGCCGCCTCTTTTACATAGTTCTGGTCATACACTTCGATAGTg                                                                                           <  1:312795/140‑1 (MQ=255)                                   gtggAAAACTGTAAGCCCAGGCGAGGAATAACCGGCGGTAACGCGTTATGCAGAACGTGGCGACGCAAAATAGTAAAGCGCGACAAACCGCGGGTCGCCGCCTCTTTTACATAGTTCTGGTCATACACTTCGATAGTGCt                                                                                         >  1:867722/1‑140 (MQ=255)                                    tggAAAACTGTAAGCCCAGGCGAGGAATAACCGGCGGTAACGCGTTATGCAGAACGTGGCGACGCAAAATAGTAAAGCGCGACAAACCGCGGGTCGCCGCCTCTTTTACATAGTTCTGGTCATACACTTCGATAGTGCTg                                                                                        >  1:873404/1‑140 (MQ=255)                                    tggAAAACTGTAAGCCCAGGCGAGGAATAACCGGCGGTAACGCGTTATGCAGAACGTGGCGACGCAAAATAGTAAAGCGCGACAAACCGCGGGTCGCCGCCTCTTTTACATAGTTCTGGTCATACACTTCGATAGTGCTg                                                                                        >  1:352202/1‑140 (MQ=255)                                     ggAAAACTGTAAGCCCAGGCGAGGAATAACCGGCGGTAACGCGTTATGCAGAACGTGGCGACGCAAAATAGTAAAGCGCGACAAACCGCGGGTCGCCGCCTCTTTTACATAGTTCTGGTCATACACTTCGATAGTGCTGa                                                                                       >  1:101023/1‑140 (MQ=255)                                      gAAAACTGTAAGCCCAGGCGAGTAATAACCGGCGGTAACGCGTTATGCAGAACGTGGCGACGCAAAATAGTAAAGCGCGACAAACCGCGGGTCGCCGCCTCTTTTACATAGTTCTGGTCATACACTTCGATAGTGCTGAt                                                                                      <  2:224149/140‑1 (MQ=255)                                      gAAAACTGTAAGCCCAGGCGAGGAATAACCGGCGGTAACGCGTTATGCAGAACGTGGCGACGCAAAATAGTAAAGCGCGACAAACCGCGGGTCGCCGCCTCTTTTACATAGTTCTGGTCATACACTTCGATAGTGCTGAt                                                                                      <  1:967709/140‑1 (MQ=255)                                         aaCTGTAATCCCAGGCGAGAAATAACCGGCGGTAACGCGTTATGCAGAACGTGGCGCCGCAAAATAGTGAAGCTCGACAAACCGCGGGTCGCGGCCTCTTTTACATAGTTCTGGTCATACACTTCGATAGTGCTGATACg                                                                                   <  2:96009/140‑1 (MQ=255)                                           cTGTAAGCCCAGGCGAGGAATAACCGGCGGTAACGCGTTATGCAGAACGTGGCGACGCAAAATAGTAAAGCGCGACAAACCGCGGGTCGCCGCCTCTTTTACATAGTTCTGGTCATACACTTCGATAGTGCTGATACGCa                                                                                 <  1:1230376/140‑1 (MQ=255)                                                     aGGCGAGGAATAACCGGCGGTAACGCGTTATGCAGAACGTGGCGACGCAAAATAGTAAAGCGCGACAAACCGCGGGTCGCCGCCTCTTTTACATAGTTCTGGTCATACACTTCGATAGTGCTGATACGCATCAGGCGGAt                                                                       <  2:86751/140‑1 (MQ=255)                                                      ggCGAGGAATAACCGGCGGTAACGCGTTATGCAGAACGTGGCGACGCAAAATAGTAAAGCGCGACAAACCGCGGGTCGCCGCCTCTTTTACATAGTTCTGGTCATACACTTCGATAGTGCTGATACGCATCAGGCGGATc                                                                      >  2:852673/1‑140 (MQ=255)                                                        cGAGGAATAACCGGCGGTAACGCGTTATGCAGAACGTGGCGACGCAAAATAGTAAAGCGCGACAAACCGCGGGTCGCCGCCTCTTTTACATAGTTCTGGTCATACACTTCGATAGTGCTGATACGCATCAGGCGGATCAc                                                                    >  2:1286600/1‑140 (MQ=255)                                                            gAATAACCGGCGGTAACGCGTTATGCAGAACGTGGCGACGCAAAATAGTAAAGCGCGACAAACCGCGGGTCGCCGCCTCTTTTACATAGTTCTGGTCATACACTTCGATAGTGCTGATACGCATCAGGCGGATCACTTCa                                                                >  2:652099/1‑140 (MQ=255)                                                                 aCCGGCGGTAACGCGTTATGCAGAACGTGGCGACGCAAAATAGTAAAGCGCGACAAACCGCGGGTCGCCGCCTCTTTTACATAGTTCTGGTCATACACTTCGATAGTGCTGATACGCATCAgg                                                                            <  2:285523/123‑1 (MQ=255)                                                                 aCCGGCGGTAACGCGTTATGCAGAACGTGGCGACGCAAAATAGTAAAGCGCGACAAACCGCGGGTCGCCGCCTCTTTTACATAGTTCTGGTCATACACTTCGATAGTGCTGATACGCATCAgg                                                                            >  1:285523/1‑123 (MQ=255)                                                                 aCCGGCGGTAACGCGTTATGCAGAACGTGGCGACGCAAAATAGTAAAGCGCGACAAACCGCGGGTCGCCGCCTCTTTTACATAGTTCTGGTCATACACTTCGATAGTGCTGATACGCATCAGGCGGATCACTTCAgttgt                                                           >  1:669049/1‑140 (MQ=255)                                                                   cggcggTAACGCGTTATGCAGAACGTGGCGACGCAAAATAGTAAAGCGCGACAAACCGCGGGTCGCCGCCTCTTTTACATAGTTCTGGTCATACACTTCGATAGTGCTGATACGCATCAGGCGGATCACTTCAGttgttg                                                         <  1:261397/140‑1 (MQ=255)                                                                   cggcggTAACGCGTTATGCAGAACGTGGCGACGCAAAATAGTAAAGCGCGACAAACCGCGGGTCGCCGCCTCTTTTACATAGTTCTGGTCATACACTTCGATAGTGCTGATACGCATCAGGCGGATCACTTCAGttgttg                                                         <  2:289672/140‑1 (MQ=255)                                                                      cggTAACGCGTTATGCAGAACGTGGCGACGCAAAATAGTAAAGCGCGACAAACCGCGGGTCGCCGCCTCTTTTACATAGTTCTGGTCATACACTTCGATAGTGCTGATACGCATCAGGCGGATCACTTCAGTTGTTGgcg                                                      >  2:1303885/1‑140 (MQ=255)                                                                      cggTAACGCGTTATGCAGAACGTGGCGACGCAAAATAGTAAAGCGCGACAAACCGCGGGTCGCCGCCTCTTTTACATAGTTCTGGTCATACACTTCGATAGTGCTGATACGCATCAGGCGGATCACTTCAGTTGTTGgcg                                                      >  1:435761/1‑140 (MQ=255)                                                                       ggTAACGCGTTATGCAGAACGTGGCGACGCAAAATAGTAAAGCGCGACAAACCGCGGGTCGCCGCCTCTTTTACATAGTTCTGGTCATACACTTCGATAGTGCTGATACGCATCAGGCGGATCACTTCAGTTGTTGgcgc                                                     <  1:1147709/140‑1 (MQ=255)                                                                       ggTAACGCGTTATGCAGAACGTGGCGACGCAAAATAGTAAAGCGCGACAAACCGCGGGTCGCCGCCTCTTTTACATAGTTCTGGTCATACACTTCGATAGTGCTGATACGCATCAGGCGGATCACTTCAGTTGTTGgcgc                                                     <  1:276167/140‑1 (MQ=255)                                                                       ggTAACGCGTTATGCAGAACGTGGCGACGCAAAATAGTAAAGCGCGACAAACCGCGGGTCGCCGCCTCTTTTACATAGTTCTGGTCATACACTTCGATAGTGCTGATACGCATCAGGCGGATCACTTCAGTTGTTGgcgc                                                     <  1:287861/140‑1 (MQ=255)                                                                       ggTAACGCGTTATGCAGAACGTGGCGACGCAAAATAGTAAAGCGCGACAAACCGCGGGTCGCCGCCTCTTTTACATAGTTCTGGTCATACACTTCGATAGTGCTGATACGCATCAGGCGGATCACTTCAGTTGTTGgcgc                                                     >  1:32542/1‑140 (MQ=255)                                                                       ggTAACGCGTTATGCAGAACGTGGCGACGCAAAATAGTAAAGCGCGACAAACCGCGGGTCGCCGCCTCTTTTACATAGTTCTGGTCATACACTTCGATAGTGCTGATACGCATCAGGCGGATCACTTCAGTTGTTGgcgc                                                     >  2:276167/1‑140 (MQ=255)                                                                         tAACGCGTTATGCAGAACGTGGCGACGCAAAATAGTAAAGCGCGACAAACCGCGGGTCGCCGCCTCTTTTACATAGTTCTGGTCATACACTTCGATAGTGCTGATACGCATCAGGCGGATCACTTCAGTTGTTGGCGCaa                                                   <  2:1199221/140‑1 (MQ=255)                                                                          aaCGCGTTATGCAGAACGTGGCGACGCAAAATAGTAAAGCGCGACAAACCGCGGGTCGCCGCCTCTTTTACATAGTTCTGGTCATACACTTCGATAGTGCTGATACGCATCAGGCGGATCACTTCAGTTGTTGGCGCAAc                                                  <  1:239761/140‑1 (MQ=255)                                                                             gcgTTATGCAGAACGTGGCGACGCAAAATAGTAAAGCGCGACAAACCGCGGGTCGCCGCCTCTTTTACATAGTTCTGGTCATACACTTCGATAGTGCTGATACGCATCAGGCGGATCACTTCAGTTGTTGGCGCAACCGa                                               <  2:140066/140‑1 (MQ=255)                                                                              cgTTATGCAGAACGTGGCGACGCAAAATAGTAAAGCGCGACAAACCGCGGGTCGCCGCCTCTTTTACATAGTTCTGGTCATACACTTCGATAGTGCTGATACGCATCAGGCGGATCACTTCAGTTGTTGGCGCAACCGAc                                              <  2:273871/140‑1 (MQ=255)                                                                                       gAACGTGGCGACGCAAAATAGTAAAGCGCGACAAACCGCGGGTCGCCGCCTCTTTTACATAGTTCTGGTCATACACTTCGATAGTGCTGATACGCATCAGGCGGATCACTTCAGTTGTTGGCGCAACCGACAGGGTGATc                                     >  2:817097/1‑140 (MQ=255)                                                                                        aaCGTGGCGACGCAAAATAGTAAAGCGCGACAAACCGCGGGTCGCCGCCTCTTTTACATAGTTCTGGTCATACACTTCGATAGTGCTGATACGCATCAGGCGGATCACTTCAGTTGTTGGCGCAACCGACAGGGTGATCa                                    <  1:482337/140‑1 (MQ=255)                                                                                        aaCGTGGCGACGCAAAATAGTAAAGCGCGACAAACCGCGGGTCGCCGCCTCTTTTACATAGTTCTGGTCATACACTTCGATAGTGCTGATACGCATCAGGCGGATCACTTCAGTTGTTGGCGCAACCGACAGGGTGATCa                                    >  2:1162416/1‑140 (MQ=255)                                                                                        aaCGTGGCGACGCAAAATAGTAAAGCGCGACAAACCGCGGGTCGCCGCCTCTTTTACATAGTTCTGGTCATACACTTCGATAGTGCTGATACGCATCAGGCGGATCACTTCAGTTGTTGGCGCAACCGACAGGGTGATCa                                    <  2:257260/140‑1 (MQ=255)                                                                                        aaCGTGGCGACGCAAAATAGTAAAGCGCGACAAACCGCGGGTCGCCGCCTCTTTTACATAGTTCTGGTCATACACTTCGATAGTGCTGATACGCATCAGGCGGATCACTTCAGTTGTTGGCGCAACCGACAGGGTGATCa                                    <  2:634210/140‑1 (MQ=255)                                                                                         aCGTGGCGACGCAAAATAGTAAAGCGCGACAAACCGCGGGTCGCCGCCTCTTTTACATAGTTCTGGTCATACACTTCGATAGTGCTGATACGCATCAGGCGGATCACTTCAGTTGTTGGCGCAACCGACAGGGTGATCAc                                   <  1:593214/140‑1 (MQ=255)                                                                                         aCGTGGCGACGCAAAATAGTAAAGCGCGACAAACCGCGGGTCGCCGCCTCTTTTACATAGTTCTGGTCATACACTTCGATAGTGCTGATACGCATCAGGCGGATCACTTCAGTTGTTGGCGCAACCGACAGGGTGATCAc                                   >  2:660876/1‑140 (MQ=255)                                                                                          cGTGGCGACGCAAAATAGTAAAGCGCGACAAACCGCGGGTCGCCGCCTCTTTTACATAGTTCTGGTCATACACTTCGATAGTGCTGATACGCATCAGGCGGATCACTTCAGTTGTTGGCGCAACCGACAGGGTGATCACg                                  <  2:873404/140‑1 (MQ=255)                                                                                          cGTGGCGACGCAAAATAGTAAAGCGCGACAAACCGCGGGTCGCCGCCTCTTTTACATAGTTCTGGTCATACACTTCGATAGTGCTGATACGCATCAGGCGGATCACTTCAGTTGTTGGCGCAACCGACAGGGTGATCACg                                  <  1:1286600/140‑1 (MQ=255)                                                                                          cGTGGCGACGCAAAATAGTAAAGCGCGACAAACCGCGGGTCGCCGCCTCTTTTACATAGTTCTGGTCATACACTTCGATAGTGCTGATACGCATCAGGCGGATCACTTCAGTTGTTGGCGCAACCGACAGGGTGATCACg                                  <  2:1105930/140‑1 (MQ=255)                                                                                            tgacGACGCAAAATAGTAAAGCGCGAAAAACCGCGGGTCGCCGCCTCTTTTACATAGTTCTGGTCATACACTTCGATAGTGCTGATACGCATCAGGCGGATCACTTCAGTTGTTGGCGCAACCGACAGGGTGATCACggg                                <  2:255180/137‑1 (MQ=255)                                                                                            tGGCGACGCAAAATAGTAAAGCGCGACAAACCGCGGGTCGCCGCCTCTTTTACATAGTTCTGGTCATACACTTCGATAGTGCTGATACGCATCAGGCGGATCACTTCAGTTGTTGGCGCAACCGACAGGGTGATCACggg                                <  2:1108081/140‑1 (MQ=255)                                                                                            tGGCGACGCAAAATAGTAAAGCGCGACAAACCGCGGGTCGCCGCCTCTTTTACATAGTTCTGGTCATACACTTCGATAGTGCTGATACGCATCAGGCGGATCACTTCAGTTGTTGGCGCAACCGACAGGGTGATCACggg                                <  1:195511/140‑1 (MQ=255)                                                                                            tGGCGACGCAAAATAGTAAAGCGCGACAAACCGCGGGTCGCCGCCTCTTTTACATAGTTCTGGTCATACACTTCGATAGTGCTGATACGCATCAGGCGGATCACTTCAGTTGTTGGCGCAACCGACAGGGTGATCACggg                                <  2:604001/140‑1 (MQ=255)                                                                                            tGGCGACGCAAAATAGTAAAGCGCGACAAACCGCGGGTCGCCGCCTCTTTTACATAGTTCTGGTCATACACTTCGATAGTGCTGATACGCATCAGGCGGATCACTTCAGTTGTTGGCGCAACCGACAGGGTGATCACggg                                <  1:954654/140‑1 (MQ=255)                                                                                            tGGCGACGCAAAATAGTAAAGCGCGACAAACCGCGGGTCGCCGCCTCTTTTACATAGTTCTGGTCATACACTTCGATAGTGCTGATACGCATCAGGCGGATCACTTCAGTTGTTGGCGCAACCGACAGGGTGATCACggg                                <  2:86222/140‑1 (MQ=255)                                                                                            tGGCGACGCAAAATAGTAAAGCGCGACAAACCGCGGGTCGCCGCCTCTTTTACATAGTTCTGGTCATACACTTCGATAGTGCTGATACGCATCAGGCGGATCACTTCAGTTGTTGGCGCAACCGACAGGGTGATCACggg                                <  1:1215645/140‑1 (MQ=255)                                                                                             ggCGACGCAAAATAGTAAAGCGCGACAAACCGCGGGTCGCCGCCTCTTTTACATAGTTCTGGTCATACACTTCGATAGTGCTGATACGCATCAGGCGGATCTCTTCAGTTGTTGGCGCAACCGACAGGGTGATCACGGGc                               >  1:646391/1‑140 (MQ=255)                                                                                             ggCGACGCAAAATAGTAAAGCGCGACAAACCGCGGGTCGCCGCCTCTTTTACATAGTTCTGGTCATACACTTCGATAGTGCTGATACGCATCAGGCGGATCACTTCAGTTGTTGGCGCAACCGACAGGGTGATCACGGGc                               >  2:121530/1‑140 (MQ=255)                                                                                              gCGACGCAAAATAGTAAAGCGCGGCAAACCGCGGGTCGCCGCCTCTTTTACATAGTTCTGGTCATACACTTCGATAGTGCTGATACGCATCAGGCGGATCACTTCAGTTGTTGGCGCAACCGACAGGGTGATCACGGGCa                              <  1:619823/140‑1 (MQ=255)                                                                                              gCGACGCAAAATAGTAAAGCGCGACAAACCGCGGGTCGCCGCCTCTTTTACATAGTTCTGGTCATACACTTCGATAGTGCTGATACGCATCAGGCGGATCACTTCAGTTGTTGGCGCAACCGACAGGGTGATCACGGGCa                              <  1:407720/140‑1 (MQ=255)                                                                                               cGACGCAAAATAGTAAAGCGCGACAAACCGCGGGTCGCCGCCTCTTTTACATAGTTCTGGTCATACACTTCGATAGTGCTGATACGCATCAGGCGGATCACTTCAGTTGTTGGCGCAACCGACAGGGTGATCACGGGCaa                             <  2:1126464/140‑1 (MQ=255)                                                                                                           gTAAAGCGCGACAAACCGCGGGTCGCCGCCTCTTTTACATAGTTCTGGTCATACACTTCGATAGTGCTGATACGCATCAGGCGGATCACTTCAGTTGTTGGCGCAACCGACAGGATGATCACGGGCAATATCATATGGCg                 <  2:707694/140‑1 (MQ=255)                                                                                                                gcgcGACAAACCGCGGGTCGCCGCCTCTTTTACATAGTTCTGGTCATACACTTCGATAGTGCTGATACGCATCAGGCGGATCACTTCAGTTGTTGGCGCAACCGACAGGGTGATCACGGGCAATATCATATGGCGGATTg            <  2:101023/140‑1 (MQ=255)                                                                                                                 cgcgACAAACCGCGGGTCGCCGCCTCTTTTACATAGTTCTGGTCATACACTTCGATAGTGCTGATACGCATCAGGCGGATCACTTCAGTTGTTGGCGCAACCGACAGGGTGATCACGGGCAATATCATATGGCGGATTgc           >  1:942558/1‑140 (MQ=255)                                                                                                                 cgcgACAAACCGCGGGTCGCCGCCTCTTTTACATAGTTCTGGTCATACACTTCGATAGTGCTGATACGCATCAGGCGGATCACTTCAGTTGTTGGCGCAACCGACAGGGTGATCACGGGCAATATCATATGGCGGATTgc           >  1:811414/1‑140 (MQ=255)                                                                                                                 cgcgACAAACCGCGGGTCGCCGCCTCTTTTACATAGTTCTGGTCATACACTTCGATAGTGCTGATACGCATCAGGCGGATCACTTCAGTTGTTGGCGCAACCGACAGGGTGATCACGGGCAATATCATATGGCGGATTgc           >  2:985994/1‑140 (MQ=255)                                                                                                                   cgACAAACCGCGGGTCGCCGCCTCTTTTACATAGTTCTGGTCATACACTTCGATAGTGCTGATACGCATCAGGCGGATCACTTCAGTTGTTGGCGCAACCGACAGGGTGATCACGGGCAATATCATATGGCGGATTgcgc         >  2:358595/1‑140 (MQ=255)                                                                                                                   cgACAAACCGCGGGTCGCCGCCTCTTTTACATAGTTCTGGTCATACACTTCGATAGTGCTGATACGCATCAGGCGGATCACTTCAGTTGTTGGCGCAACCGACAGGGTGATCACGGGCAATATCATATGGCGGATTgcgc         >  2:800011/1‑140 (MQ=255)                                                                                                                    gACAAACCGCGGGTCGCCGCCTCTTTTACATAGTTCTGGTCATACACTTCGATAGTGCTGATACGCATCAGGCGGATCACTTCAGTTGTTGGCGCAACCGACAGGGTGATCACGGGCAATATCATATGGCGGATTGCGCt        >  1:200885/1‑140 (MQ=255)                                                                                                                     aCAAACCGCGGGTCGCCGCCTCTTTTACATAGTTCTGGTCATACACTTCGATAGTGCTGATACGCATCAGGCGGATCACTTCAGTTGTTGGCGCAACCGACAGGGTGATCACGGGCAATATCATATGGCGGATTGCGCTc       >  2:432263/1‑140 (MQ=255)                                                                                                                         aCCGCGGGTCGCCGCCTCTTTTACATAGTTCTGGTCATACACTTCGATAGTGCTGATACGCATCAGGCGGATCACTTCAGTTGTTGGCGCAACCGACAGGGTGATCACGGGCAATATCATATGGCGGATTGCGCTCATGa   <  2:972969/140‑1 (MQ=255)                                                                                                                          ccGCGGGTCGCCGCCTCTTTTACATAGTTCTGGTCATACACTTCGATAGTGCTGATACGCATCAGGCGGATCACTTCAGTTGTTGGCGCAACCGACAGGGTGATCACGGGCAATATCATATGGCGGATTGAGCTCATGAt  <  2:226538/140‑1 (MQ=255)                                                                                                                                         |                                                                                                                             AACCATTTCGGTGATCATCGCCAGCGTCAGCATGGTGGAAAACTGTAAGCCCAGGCGAGGAATAACCGGCGGTAACGCGTTATGCAGAACGTGGCGACGCAAAATAGTAAAGCGCGACAAACCGCGGGTCGCCGCCGCTTTTACATAGTTCTGGTCATACACTTCGATAGTGCTGATACGCATCAGGCGGATCACTTCAGTTGTTGGCGCAACCGACAGGGTGATCACGGGCAATATCATATGGCGGATTGCGCTCATGAT  >  NC_000913/1354685‑1354945

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑