Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,581,168	G→T	P453T (CCA→ACA)	ydeN ←	putative sulfatase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,581,168	0	G	T	100.0%	269.6 / NA	78	P453T (CCA→ACA)	ydeN	putative sulfatase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (46/32);  total (46/32)

GTTTTCTACTGTATAGACAAGCGAATAATCGTTATTTCTCACCGTATAAGAGAATTGGCTTAAGTCCTCAGTGTTGGGGTTATGCGGGTAATCGTCTGACTGATGGCGAACAAATTTGTGGTAATTATCCCAGAATGGAATATTTTCCTCGTCAAACCAGTGAGAATAAGAGGTTATCCAGGTCAGATTTTTATGTGGCTCGCCTTGTTTCTTATCTTGCAACCAGGGCAGCAAGGAAACGCCATCCAGCTTAAGGTCTTTTGGAATGCTG  >  NC_000913/1581031‑1581301                                                                                                                                          |                                                                                                                                      gTTTTCTACTGTATAGACAAGCGAATAATCGTTATTTCTCACCGTATAAGAGAATTGGCTTAAGTCCTCAGTGTTGGGGTTATGCGGGTAATCGTCTGACTGATGGCGAACAAATTTGTGGTAATTATCCCAGAATGTaa                                                                                                                                     >  1:394217/1‑140 (MQ=255)        aCTGTATAGACAAGCGAATAATCGTTATTTCTCACCGTATAAGAGAATTGGCTTAAGTCCTCAGTGTTGGGGTTGTGCGGGTAATCGTCTGACTGATGGCGAACAAATTTGTGGTAATTATCCCAGAATGTAATATTTTc                                                                                                                              >  1:73751/1‑140 (MQ=255)        aCTGTATAGACAAGCGAATAATCGTTATTTCTCACCGTATAAGAGAATTGGCTTAAGTCCTCAGTGTTGGGGTTATGCGGGTAATCGTCTGACTGATGGCGAACAAATTTGTGGTAATTATCCCAGAATGTAATATTTTc                                                                                                                              <  1:681913/140‑1 (MQ=255)            tataGACAAGCGAATAATCGTTATTTCTCACCGTATAAGAGAATTGGCTTAAGTCCTCAGTGTTGGGGTTATGCGGGTAATCGTCTGACTGATGGCGAACAAATTTGTGGTAATTATCCCAGAATGTAATATTTTCCTCg                                                                                                                          >  2:723706/1‑140 (MQ=255)             ataGACAAGCGAATAATCGTTATTTCTCACCGTATAAGAGAATTGGCTTAAGTCCTCAGTGTTGGGGTTATGCGGGTAATCGTCTGACTGATGGCGAACAAATTTGTGGTAATTATCCCAGAATGTAATATTTTCCTCGt                                                                                                                         <  2:953295/140‑1 (MQ=255)              taGACAAGCGAATAATCGTTATTTCTCACCGTATAAGAGAATTGGCTTAAGTCCTCAGTGTTGGGGTTATGCGGGTAATCGTCTGACTGATGGCGAACAAATTTGTGGTAATTATCCCAGAATGTAATATTTTCCTCGTc                                                                                                                        >  2:1183888/1‑140 (MQ=255)              taGACAAGCGAATAATCGTTATTTCTCACCGTATAAGAGAATTGGCTTAAGTCCTCAGTGTTGGGGTTATGCGGGTAATCGTCTGACTGATGGCGAACAAATTTGTGGTAATTATCCCAGAATGTAATATTTTCCTCGTc                                                                                                                        >  1:1187180/1‑140 (MQ=255)                    aGCGAATAATCGTTATTTCTCACCGTATAAGAGAATTGGCTTAAGTCCTCAGTGTTGGGGTTATGCGGGTAATCGTCTGACTGATGGCGAACAAATTTGTGGTAATTATCCCAGAATGTAATATTTTCCTCGTCAAACCa                                                                                                                  <  2:787917/140‑1 (MQ=255)                      cGAATAATCGTTATTTCTCACCGTATAAGAGAATTGGCTTAAGTCCTCAGTGTTGGGGTTATGCGGGTAATCGTCTGACTGATGGCGAACAAATTTGTGGTAATTATCCCAGAATGTAATATTTTCCTTGTCAAACCAGt                                                                                                                >  1:1042557/1‑140 (MQ=255)                      cGAATAATCGTTATTTCTCACCGTATAAGAGAATTGGCTTAAGTCCTCAGTGTTGGGGTTATGCGGGTAATCGTCTGACTGATGGCGAACAAATTTGTGGTAATTATCCCAGAATGTAATATTTTCCTCGTCAAACCAGt                                                                                                                >  1:204226/1‑140 (MQ=255)                      cGAATAATCGTTATTTCTCACCGTATAAGAGAATTGGCTTAAGTCCTCAGTGTTGGGGTTATGCGGGTAATCGTCTGACTGATGGCGAACAAATTTGTGGTAATTATCCCAGAATGTAATATTTTCCTCGTCAAACCAGt                                                                                                                >  2:134165/1‑140 (MQ=255)                      cGAATAATCGTTATTTCTCACCGTATAAGAGAATTGGCTTAAGTCCTCAGTGTTGGGGTTATGCGGGTAATCGTCTGACTGATGGCGAACAAATTTGTGGTAATTATCCCAGAATGTAATATTTTCCTCGTCAAACCAGt                                                                                                                >  1:844594/1‑140 (MQ=255)                      cGAATAATCGTTATTTCTCACCGTATAAGAGAATTGGCTTAAGTCCTCAGTGTTGGGGTTATGCGGGTAATCGTCTGACTGATGGCGAACAAATTTGTGGTAATTATCCCAGAATGTAATATTTTCCTCGTCAAACCAGt                                                                                                                <  1:373530/140‑1 (MQ=255)                      cGAATAATCGTTATTTCTCACCGTATAAGAGAATTGGCTTAAGTCCTCAGTGTTGGGGTTATGCGGGTAATCGTCTGACTGATGGCGAACAAATTTGTGGTAATTATCCCAGAATGTAATATTTTCCTCGTCAAACCAGt                                                                                                                >  2:373530/1‑140 (MQ=255)                      cGAATAATCGTTATTTCTCACCGTATAAGAGAATTGGCTTAAGTCCTCAGTGTTGGGGTTATGCGGGTAATCGTCTGACTGATGGCGAACAAATTTGTGGTAATTATCCCAGAATGTAATATTTTCCTCGTCAAACCAGt                                                                                                                >  2:1190617/1‑140 (MQ=255)                      cGAATAATCGTTATTTCTCACCGTATAAGAGAATTGGCTTAAGTCCTCAGTGTTGGGGTTATGCGGGTAATCGTCTGACTGATGGCGAACAAATTTGTGGTAATTATCCCAGAATGTAATATTTTCCTCGTCAAACCAGt                                                                                                                >  2:604957/1‑140 (MQ=255)                      cGAATAATCGTTATTTCTCACCGTATAAGAGAATTGGCTTAAGTCCTCAGTGTTGGGGTTATGCGGGTAATCGTCTGACTGATGGCGAACAAATTTGTGGTAATTATCCCAGAATGTAATATTTTCCTCGTCAAACCAGt                                                                                                                >  2:910698/1‑140 (MQ=255)                        aataatCGTTATTTCTCACCGTATAAGAGAATTGGCTTAAGTCCTCAGTGTTGGGGTTATGCGGGTAATCGTCTGACTGATGGCGAACAAATTTGTGGTAATTATCCCAGAATGTAATATTTTCCTCGTCAAACCAGTga                                                                                                              <  2:394217/140‑1 (MQ=255)                           aatCGTTATTTCTCACCGTATAAGAGAATTGGCTTAAGTCCTCAGTGTTGGGGTTATGCGGGTAATCGTCTGACTGATGGCGAACAAATTTGTGGTAATTATCCCAGAATGTAATATTTTCCTCGTCAAACCAGTGAGaa                                                                                                           >  2:167757/1‑140 (MQ=255)                             tCGTTATTTCTCACCGTATAAGAGAATTGGCTTAAGTCCTCAGTGTTGGGGTTATGCGGGTAATCGTCTGACTGATGGCGAACAAATTTGTGGTAATTATCCCAGAATGTAATATTTTCCTCGTCAAACCAGTGAGAATa                                                                                                         <  2:1095053/140‑1 (MQ=255)                                ttATTTCTCACCGTATAAGAGAATTGGCTTAAGTCCTCAGTGTTGGGGTTATGCGGGTAATCGTCTGACTGATGGCGAACAAATTTGTGGTAATTATCCCAGAATGTAATATTTTCCTCGTCAAACCAGTGAGAATAaga                                                                                                      >  1:1176220/1‑140 (MQ=255)                                  aTTTCTCACCGTATAAGAGAATTGGCTTAAGTCCTCAGTGTTGGGGTTATGCGGGTAATCGTCTGACTGATGGCGAACAAATTTGTGGTAATTATCCCAGAATGTAATATTTTCCTCGTCAAACCAGTGAGAATAAGAgg                                                                                                    <  1:723706/140‑1 (MQ=255)                                   tttCTCACCGTATAAGAGAATTGGCTTAAGTCCTCAGTGTTGGGGTTATGCGGGTAATCGTCTGACTGATGGCGAACAAATTTGTGGTAATTATCCCAGAATGTAATATTTTCCTCGTCAAACCAGTGAGAATAAGAGGt                                                                                                   >  1:1220666/1‑140 (MQ=255)                                        cACCGTATAAGAGAATTGGCTTAAGTCCTCAGTGTTGGGGTTATGCGGGTAATCGTCTGACTGATGGCGAACAAATTTGTGGTAATTATCCCAGAATGTAATATTTTCCTCGTCAAACCAGTGAGAATAAGAGGTTATcc                                                                                              >  1:319522/1‑140 (MQ=255)                                             tataAGAGAATTGGCTTAAGTCCTCAGTGTTGGGGTTATGCGGGTAATCGTCTGACTGATGGCGAACAAATTTGTGGTAATTATCCCAGAATGTAATATTTTCCTCGTCAAACCAGTGAGAATAAGAGGTTATCCAGGTc                                                                                         <  1:167757/140‑1 (MQ=255)                                             tataAGAGAATTGGCTTAAGTCCTCAGTGTTGGGGTTATGCGGGTAATCGTCTGACTGATGGCGAACAAATTTGTGGTAATTATCCCAGAATGTAATATTTTCCTCGTCAAACCAGTGAGAATAAGAGGTTATCCAGGTc                                                                                         >  1:77050/1‑140 (MQ=255)                                              ataAGAGAATTGGCTTAAGTCCTCAGTGTTGGGGTTATGCGGGTAATCGTCTGACTGATGGCGAACAAATTTGTGGTAATTATCCCAGAATGTAATATTTTCCTCGTCAAACCAGTGAGAATAAGAGGTTATCCAGGTCa                                                                                        <  1:1228632/140‑1 (MQ=255)                                              ataAGAGAATTGGCTTAAGTCCTCAGTGTTGGGGTTATGCGGGTAATCGTCTGACTGATGGCGAACAAATTTGTGGTAATTATCCCAGAATGTAATATTTTCCTCGTCAAACCAGTGAGAATAAGAGGTTATCCAGGTCa                                                                                        <  2:1124360/140‑1 (MQ=255)                                               taAGAGAATTGGCTTAAGTCCTCAGTGTTGGGGTTATGCGGGTAATCGTCTGACTGATGGCGAACAAATTTGTGGTAATTATCCCAGAATGTAATATTTTCCTCGTCAAACCAGTGAGAATAAGAgg                                                                                                    <  1:1035443/127‑1 (MQ=255)                                               taAGAGAATTGGCTTAAGTCCTCAGTGTTGGGGTTATGCGGGTAATCGTCTGACTGATGGCGAACAAATTTGTGGTAATTATCCCAGAATGTAATATTTTCCTCGTCAAACCAGTGAGAATAAGAgg                                                                                                    >  2:1035443/1‑127 (MQ=255)                                               taAGAGAATTGGCTTAAGTCCTCAGTGTTGGGGTTATGCGGGTAATCGTCTGACTGATGGCGAACAAATTTGTGGTAATTATCCCAGAATGTAATATTTTCCTCGTCAAACCAGTGAGAATAAGAGGTTATCCAGGTCAg                                                                                       >  1:84346/1‑140 (MQ=255)                                                     aaTTGGCTTAAGTCCTCAGTGTTGGGGTTATGCGGGTAATCGTCTGACTGATGGCGAACAAATTTGTGGTAATTATCCCAGAATGTAATATTTTCCTCGTCAAACCAGTGAGAATAAGAGGTTATCCAGGTCAGAttttt                                                                                 >  1:657050/1‑140 (MQ=255)                                                     aaTTGGCTTAAGTCCTCAGTGTTGGGGTTATGCGGGTAATCGTCTGACTGATGGCGAACAAATTTGTGGTAATTATCCCAGAATGTAATATTTTCCTCGTCAAACCAGTGAGAATAAGAGGTTATCCAGGTCAGAttttt                                                                                 >  1:29178/1‑140 (MQ=255)                                                      aTTGGCTTAAGTCCTCAGTGTTGGGGTTATGCGGGTAATCGTCTGACTGATGGCGAACAAATTTGTGGTAATTATCCCAGAATGTAATATTTTCCTCGTCAAACCAGTGAGAATAAGAGGTTATCCAGGTCAGATTTTTa                                                                                >  1:122255/1‑140 (MQ=255)                                                       ttGGCTTAAGTCCTCAGTGTTGGGGTTATGCGGGTAATCGTCTGACTGATGGCGAACAAATTTGTGGTAATTATCCCAGAATGTAATATTTTCCTCGTCAAACCAGTGAGAATAAGAGGTTATCCAGGTCAGATTTTTAt                                                                               >  1:46915/1‑140 (MQ=255)                                                          gCTTAAGTCCTCAGTGTTGGGGTTATGCGGGTAATCGTCTGACTGATGGCGAACAAATTTGTGGTAATTATCCCAGAATGTAATATTTTCCTCGTCAAACCAGTGAGAATAAGAGGTTATCCAGGTCAGATTTTTAtgtg                                                                            >  1:1308435/1‑140 (MQ=255)                                                            ttAAGTCCTCAGTGTTGGGGTTATGCGGGTAATCGTCTGACTGATGGCGAACAAATTTGTGGTAATTATCCCAGAATGTAATATTTTCCTCGTCAAACCAGTGAGAATAAGAGGTTATCCAGGTCAGATTTTTATGTGGc                                                                          <  1:1190617/140‑1 (MQ=255)                                                             tAAGTCCTCAGTGTTGGGGTTATGCGGGTAATCGTCTGACTGATGGCGAACAAATTTGTGGTAATTATCCCAGAATGTAATATTTTCCTCGTCAAACCAGTGAGAATAAGAGGTTATCCAGGTCAGATTTTTATGTGGCt                                                                         <  1:604957/140‑1 (MQ=255)                                                             tAAGTCCTCAGTGTTGGGGTTATGCGGGTAATCGTCTGACTGATGGCGAACAAATTTGTGGTAATTATCCCAGAATGTAATATTTTCCTCGTCAAACCAGTGAGAATAAGAGGTTATCCAGGTCAGATTTTTATGTGGCt                                                                         <  1:910698/140‑1 (MQ=255)                                                               aGTCCTCAGTGTTGGGGTTATGCGGGTAATCGTCTGACTGATGGCGAACAAATTTGTGGTAATTATCCCAGAATGTAATATTTTCCTCGTCAAACCAGTGAGAATAAGAGGTTATCCAGGTCAGATTTTTATGTGGCTCg                                                                       >  1:1266850/1‑140 (MQ=255)                                                                  ccTCAGTGTTGGGGTTATGCGGGTAATCGTCTGACTGATGGCGAACAAATTTGTGGTAATTATCCCAGAATGTAATATTTTCCTCGTCAAACCAGTGAGAATAAGAGGTTATCCAGGTCAGATTTTTATGTGGCTCGCCt                                                                    <  1:642706/140‑1 (MQ=255)                                                                  ccTCAGTGTTGGGGTTATGCGGGTAATCGTCTGACTGATGGCGAACAAATTTGTGGTAATTATCCCAGAATGTAATATTTTCCTCGTCAAACCAGTGAGAATAAGAGGTTATCCAGGTCAGATTTTTATGTGGCTCGCCt                                                                    <  2:292744/140‑1 (MQ=255)                                                                  ccTCAGTGTTGGGGTTATGCGGGTAATCGTCTGACTGATGGCGAACAAATTTGTGGTAATTATCCCAGAATGTAATATTTTCCTCGTCAAACCAGTGAGAATAAGAGGTTATCCAGGTCAGATTTTTATGTGGCTCGCCt                                                                    >  2:866655/1‑140 (MQ=255)                                                                    tCAGTGTTGGGGTTATGCGGGTAATCGTCTGACTGATGGCGAACAAATTTGTGGTAATTATCCCAGAATGTAATATTTTCCTCGTCAAACCAGTGAGAATAAGAGGTTATCCAGGTCAGATTTTTATGTGGCTCGCCTTg                                                                  >  2:1033730/1‑140 (MQ=255)                                                                          ttGGGGTTATGCGGGTAATCGTCTGACTGATGGCGAACAAATTTGTGGTAATTATCCCAGAATGTAATATTTTCCTCGTCAAACCAGTGAGAATAAGAGGTTATCCAGGTCAGATTTTTATGTGGCTCGCCTTGTTTCtt                                                            <  2:1227062/140‑1 (MQ=255)                                                                          ttGGGGTTATGCGGGTAATCGTCTGACTGATGGCGAACAAATTTGTGGTAATTATCCCAGAATGTAATATTTTCCTCGTCAAACCAGTGAGAATAAGAGGTTATCCAGGTCAGATTTTTATGTGGCTCGCCTTGTTTCtt                                                            >  2:824519/1‑140 (MQ=255)                                                                             gggTTATGCGGGTAATCGTCTGACTGATGGCGAACAAATTTGTGGTAATTATCCCAGAATGTAATATTTTCCTCGTCAAACCAGTGAGAATAAGAGGTTATCCAGGTCAGATTTTTATGTGGCTCGCCTTGTTTCTTATc                                                         >  2:1166427/1‑140 (MQ=255)                                                                               gTTATGCGGGTAATCGTCTGACTGATGGCGAACAAATTTGTGGTAATTATCCCAGAATGTAATATTTTCCTCGTCAAACCAGTGAGAATAAGAGGTTATCCAGGTCAGATTTTTATGTGGCTCGCCTTGTTTCTTATCtt                                                       <  1:768551/140‑1 (MQ=255)                                                                                  aTGCGGGTAATCGTCTGACTGATGGCGAACAAATTTGTGGTAATTATCCCAGAATGTAATATTTTCCTCGTCAAACCAGTGAGAATAAGAGGTTATCCAGGTCAGATTTTTATGTGGCTCGCCTTGTTTCTTATCTTGCa                                                    >  1:585090/1‑140 (MQ=255)                                                                                  aTGCGGGTAATCGTCTGACTGATGGCGAACAAATTTGTGGTAATTATCCCAGAATGTAATATTTTCCTCGTCAAACCAGTGAGAATAAGAGGTTATCCAGGTCAGATTTTTATGTGGCTCGCCTTGTTTCTTATCTTGCa                                                    >  2:332385/1‑140 (MQ=255)                                                                                  aTGCGGGTAATCGTCTGACTGATGGCGAACAAATTTGTGGTAATTATCCCAGAATGTAATATTTTCCTCGTCAAACCAGTGAGAATAAGAGGTTATCCAGGTCAGATTTTTATGTGGCTCGCCTTGTTTCTTATCTTGCa                                                    >  1:1137218/1‑140 (MQ=255)                                                                                  aTGCGGGTAATCGTCTGACTGATGGCGAACAAATTTGTGGTAATTATCCCAGAATGTAATATTTTCCTCGTCAAACCAGTGAGAATAAGAGGTTATCCAGGTCAGATTTTTATGTGGCTCGCCTTGTTTCTTATCTTGCa                                                    >  2:474725/1‑140 (MQ=255)                                                                                       ggTAATCGTCTGACTGATGGCGAACAAATTTGTGGTAATTATCCCAGAATGTAATATTTTCCTCGTCAAACCAGTGAGAATAAGAGGTTATCCAGGTCAGATTTTTATGTGGCTCGCCTTGTTTCTTATCTTGCAACCAg                                               <  2:585090/140‑1 (MQ=255)                                                                                         tAATCGTCTGACTGATGGCGAACAAATTTGTGGTAATTATCCCAGAATGTAATATTTTCCTCGTCAAACCAGTGAGAATAAGAGGTTATCCAGGTCAGATTTTTATGTGGCTCGCCTTGTTTCTTATCTTGCAACCAggg                                             >  2:716887/1‑140 (MQ=255)                                                                                         tAATCGTCTGACTGATGGCGAACAAATTTGTGGTAATTATCCCAGAATGTAATATTTTCCTCGTCAAACCAGTGAGAATAAGAGGTTATCCAGGTCAGATTTTTATGTGGCTCGCCTTGTTTCTTATCTTGCAACCAggg                                             >  2:317900/1‑140 (MQ=255)                                                                                          aaTCGTCTGACTGATGGCGAACAAATTTGTGGTAATTATCCCAGAATGTAATATTTTCCTCGTCAAACCAGTGAGAATAAGAGGTTATCCAGGTCAGATTTTTATGTGGCTCGCCTTGTTTCTTATCTTGCAACCAGGgc                                            >  1:679418/1‑140 (MQ=255)                                                                                             cGTCTGACTGATGGCGAACAAATTTGTGGTAATTATCCCAGAATGTAATATTTTCCTCGTCAAACCAGTGAGAATAAGAGGTTATCCAGGTCAGATTTTTATGTGGCTCGCCTTGTTTCTTATCTTGCAACCAGGgcagc                                         >  1:981248/1‑140 (MQ=255)                                                                                             cGTCTGACTGATGGCGAACAAATTTGTGGTAATTATCCCAGAATGTAATATTTTCCTCGTCAAACCAGTGAGAATAAGAGGTTATCCAGGTCAGATTTTTATGTGGCTCGCCTTGTTTCTTATCTTGCAACCAGGgcagc                                         <  2:87955/140‑1 (MQ=255)                                                                                                      gaTGGCGAACAAATTTGTGGTAATTATCCCAGAATGTAATATTTTCCTCGTCAAACCAGTGAGAATAAGAGGTTATCCAGGTCAGATTTTTATGTGGCTCGCCTTGTTTCTTATCTTGCAACCAGGGCAGCAAGGAAACg                                <  2:679418/140‑1 (MQ=255)                                                                                                      gaTGGCGAACAAATTTGTGGTAATTATCCCAGAATGTAATATTTTCCTCGTCAAACCAGTGAGAATAAGAGGTTATCCAGGTCAGATTTTTATGTGGCTCGCCTTGTTTCTTATCTTGCAACCAGGGCAGCAAGGAAACg                                <  2:981248/140‑1 (MQ=255)                                                                                                      gaTGGCGAACAAATTTGTGGTAATTATCCCAGAATGTAATATTTTCCTCGTCAAACCAGTGAGAATAAGAGGTTATCCAGGTCAGATTTTTATGTGGCTCGCCTTGTTTCTTATCTTGCAACCAGGGCAGCAAGGAAACg                                <  1:866478/140‑1 (MQ=255)                                                                                                       aTGGCGAACAAATTTGTGGTAATTATCCCAGAATGTAATATTTTCCTCGTCAAACCAGTGAGAATAAGAGGTTATCCAGGTCAGATTTTTATGTGGCTCGCCTTGTTTCTTATCTTGCAACCAGGGCAGCAAGGAAACGc                               >  2:598346/1‑140 (MQ=255)                                                                                                       aTGGCGAACAAATTTGTGGTAATTATCCCAGAATGTAATATTTTCCTCGTCAAACCAGTGAGAATAAGAGGTTATCCAGGTCAGATTTTTATGTGGCTCGCCTTGTTTCTTATCTTGCAACCAGGGCAGCAAGGAAACGc                               >  1:733313/1‑140 (MQ=255)                                                                                                       aTGGCGAACAAATTTGTGGTAATTATCCCAGAATGTAATATTTTCCTCGTCAAACCAGTGAGAATAAGAGGTTATCCAGGTCAGATTTTTATGTGGCTCGCCTTGTTTCTTATCTTGCAACCAGGGCAGCAAGGAAACGc                               >  2:627303/1‑140 (MQ=255)                                                                                                           cGAACAAATTTGTGGTAATTATCCCAGAATGTAATATTTTCCTCGTCAAACCAGTGAGAATAAGAGGTTATCCAGGTCAGATTTTTATGTGGCTCGCCTTGTTTCTTATCTTGCAACCAGGGCAGCAAGGAAACGCCATc                           <  1:971845/140‑1 (MQ=255)                                                                                                           cGAACAAATTTGTGGTAATTATCCCAGAATGTAATATTTTCCTCGTCAAACCAGTGAGAATAAGAGGTTATCCAGGTCAGATTTTTATGTGGCTCGCCTTGTTTCTTATCTTGCAACCAGGGCAGCAAGGAAACGCCATc                           <  2:733313/140‑1 (MQ=255)                                                                                                                 aaTTTGTGGTAATTATCCCAGAATGTAATATTTTCCTCGTCAAACCAGTGAGAATAAGAGGTTATCCAGGTCAGATTTTTATGTGGCTCGCCTTGTTTCTTATCTTGCAACCAGGGCAGCAAGGAAACGCCATCCAGCtt                     >  2:797534/1‑140 (MQ=255)                                                                                                                  aTTTGTGGTAATTATCCCAGAATGTAATATTTTCCTCGTCAAACCAGTGAGAATAAGAGGTTATCCAGGTCAGATTTTTATGTGGCTCGCCTTGTTTCTTATCTTGCAACCAGGGCAGCAAGGAAACGCCATCCAGCTTa                    <  2:844594/140‑1 (MQ=255)                                                                                                                    ttGTGGTAATTATCCCAGAATGTAATATTTTCCTCGTCAAACCAGTGAGAATAAGAGGTTATCCAGGTCAGATTTTTATGTGGCTCGCCTTGTTTCTTATCTTGCAACCAGGGCAGCAAGGAAACGCCATCCAGCTTAAg                  <  1:955616/140‑1 (MQ=255)                                                                                                                    ttGTGGTAATTATCCCAGAATGTAATATTTTCCTCGTCAAACCAGTGAGAATAAGAGGTTATCCAGGTCAGATTTTTATGTGGCTCGCCTTGTTTCTTATCTTGCAACCAGGGCAGCAAGGAAACGCCATCCAGCTTAAg                  <  1:310770/140‑1 (MQ=255)                                                                                                                       tgGTAATTATCCCAGAATGTAATATTTTCCTCGTCAAACCAGTGAGAATAAGAGGTTATCCAGGTCAGATTTTTATGTGGCTCGCCTTGTTTCTTATCTTGCAACCAGGGCAGCAAGGAAACGCCATCCAGCTTAAGGTc               <  2:1176220/140‑1 (MQ=255)                                                                                                                            aTTATCCCAGAATGTAATATTTTCCTCGTCAAACCAGTGAGAATAAGAGGTTATCCAGGTCAGATTTTTATGTGGCTCGCCTTGTTTCTTATCTTGCAACCAGGGCAGCAAGGAAACGCCATCCAGCTTAAGGTCTTTTg          <  2:1220666/140‑1 (MQ=255)                                                                                                                            aTTATCCCAGAATGTAATATTTTCCTCGTCAAACCAGTGAGAATAAGAGGTTATCCAGGTCAGATTTTTATGTGGCTCGCCTTGTTTCTTATCTTGCAACCAGGGCAGCAAGGAAACGCCATCCAGCTTAAGGTCTTTTg          >  1:507420/1‑140 (MQ=255)                                                                                                                            aTTATCCCAGAATGTAATATTTTCCTCGTCAAACCAGTGAGAATAAGAGGTTATCCAGGTCAGATTTTTATGTGGCTCGCCTTGTTTCTTATCTTGCAACCAGGGCAGCAAGGAAACGCCATCCAGCTTAAGGTCTTTTg          >  1:1132235/1‑140 (MQ=255)                                                                                                                            aTTATCCCAGAATGTAATATTTTCCTCGTCAAACCAGTGAGAATAAGAGGTTATCCAGGTCAGATTTTTATGTGGCTCGCCTTGTTTCTTATCTTGCAACCAGGGCAGCAAGGAAACGCCATCCAGCTTAAGGTCTTTTg          <  1:1033730/140‑1 (MQ=255)                                                                                                                              tATCCCAGAATGTAATATTTTCCTCGTCAAACCAGTGAGAATAAGAGGTTATCCAGGTCAGATTTTTATGTGGCTCGCCTTGTTTCTTATCTTGCAACCAGGGCAGCAAGGAAACGCCATCCAGCTTAAGGTCTTTTGGa        <  2:77050/140‑1 (MQ=255)                                                                                                                                  ccAGAATGTAATATTTTCCTCGTCAAACCAGTGAGAATAAGAGGTTATCCAGGTCAGATTTTTATGTGGCTCGCCTTGTTTCTTATCTTGCAACCAGGGCAGCAAGGAAACGCCATCCAGCTTAAGGTCTTTTGGAATGc    >  1:1107662/1‑140 (MQ=255)                                                                                                                                    aGAATGTAATATTTTCCTCGTCAAACCAGTGAGAATAAGAGGTTATCCAGGTCAGATTTTTATGTGGCTCGCCTTGTTTCTTATCTTGCAACCAGGGCAGCAAGGAAACGCCATCCAGCTTAAGGTCTTTTGGAATGCTg  >  2:1185038/1‑140 (MQ=255)                                                                                                                                          |                                                                                                                                      GTTTTCTACTGTATAGACAAGCGAATAATCGTTATTTCTCACCGTATAAGAGAATTGGCTTAAGTCCTCAGTGTTGGGGTTATGCGGGTAATCGTCTGACTGATGGCGAACAAATTTGTGGTAATTATCCCAGAATGGAATATTTTCCTCGTCAAACCAGTGAGAATAAGAGGTTATCCAGGTCAGATTTTTATGTGGCTCGCCTTGTTTCTTATCTTGCAACCAGGGCAGCAAGGAAACGCCATCCAGCTTAAGGTCTTTTGGAATGCTG  >  NC_000913/1581031‑1581301

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑