Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,323,339	C→A	intergenic (+88/‑108)	atoC → / → atoD	DNA‑binding transcriptional activator/ornithine decarboxylase inhibitor AtoC/acetyl‑CoA:acetoacetyl‑CoA transferase subunit alpha

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,323,339	0	C	A	100.0%	93.2 / NA	29	intergenic (+88/‑108)	atoC/atoD	DNA‑binding transcriptional activator/ornithine decarboxylase inhibitor AtoC/acetyl‑CoA:acetoacetyl‑CoA transferase subunit alpha
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (14/15);  total (14/15)

TCCAGGAATACGGTATCGATCCGGCGGATGTATAACACCAAAACTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCATATTCAATTAGTTAAATAACTAAATCCAATAATCTCATTCTGGCACTCCCCTTGCTATTGCCTGACTGTACCCACAACGGTGTATGCAAGAGGGATAAAAAATGAAAACAAAATTGATGAC  >  NC_000913/2323217‑2323466                                                                                                                           |                                                                                                                                tCCAGGAATACGGTATCGATCCGGCGGATGTATAACACCAAAACTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATACCTTCATATTCAATTAg                                                                                                                <  1:212964/140‑1 (MQ=255)  ccAGGAATACGGTATCGATCCGGCGGATGTATAACACCAAAACTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATACCTTCATATTCAATTAGt                                                                                                               >  1:269501/1‑140 (MQ=255)   cAGGAATACGGTATCGATCCGGCGGATGTATAACACCAAAACTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATACCTTCATATTCAATTAGtt                                                                                                              >  2:215069/1‑140 (MQ=255)   cAGGAATACGGTATCGATCCGGCGGATGTATAACACCAAAACTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATACCTTCATATTCAATTAGtt                                                                                                              >  2:153577/1‑140 (MQ=255)    aGGAATACGGTATCGATCCGGCGGATGGATAACACCAAAACTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATACCTTCATATTCAATTAGTTa                                                                                                             >  2:94570/1‑140 (MQ=255)            ggTATCGATCCGGCGGATGTATAACACCAAAACTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATACCTTCATATTCAATTAGTTAAATAACTa                                                                                                     <  1:94570/140‑1 (MQ=255)                tCGATCCGGCGGATGTATAACACCAAAACTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATACCTTCATATTCAATTAGTTaaa                                                                                                           >  2:382644/1‑130 (MQ=255)                tCGATCCGGCGGATGTATAACACCAAAACTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATACCTTCATATTCAATTAGTTaaa                                                                                                           <  1:382644/130‑1 (MQ=255)                tCGATCCGGCGGATGTATAACACCAAAACTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATACCTTCATATTCAATTAGTTAAATAACTAAATc                                                                                                 >  2:128111/1‑140 (MQ=255)                   aTCCGGCGGATGTATAACACCAAAACTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATACCTTCATATTCAATTAGTTAAATAACTAAATCCaa                                                                                              <  1:128111/140‑1 (MQ=255)                       ggcggATGTATAACACCAAAACTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATACCTTCATATTCAATTAGTTAAATAACTAAATCCaataat                                                                                          <  2:269501/140‑1 (MQ=255)                        gcggATGTATAACACCAAAACTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATACCTTCATATTCAATTAGTTAAATAACTAAATCCAATAAtc                                                                                         >  2:78242/1‑140 (MQ=255)                                     acCAAAACTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATACCTTCATATTCAATTAGTTAAATAACTAAATCCAATAATCTCATTCTGGCACt                                                                            >  1:133262/1‑140 (MQ=255)                                      ccAAAACTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATACCTTCATATTCAATTAGTTAAATAACTAAATCCAATAATCTCATTCTGGCACTc                                                                           >  2:222568/1‑140 (MQ=255)                                                    gCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATACCTTCATATTCAATTAGTTAAATAACTAAATCCAATAATCTCATTCTGGCACTCCCCTTGCTATTGcc                                                             <  2:79875/140‑1 (MQ=255)                                                    gCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATACCTTCATATTCAATTAGTTAAATAACTAAATCCAATAATCTCATTCTGGCACTCCCCTTGCTATTGcc                                                             <  2:133262/140‑1 (MQ=255)                                                             tGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATACCTTCATATTCAATTAGTTAAATAACTAAATCCAATAATCTCATTCTGGCACTCCCCTTGCTATTGCCTGACTGTAc                                                    <  1:78242/140‑1 (MQ=255)                                                              gCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATACCTTCATATTCAATTAGTTAAATAACTAAATCCAATAATCTCATTCTGGCACTCCCCTTGCTATTGCCTGACTGTAcc                                                   <  1:239271/140‑1 (MQ=255)                                                                  aGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATACCTTCATATTCAATTAGTTAAATAACTAAATCCAATAATCTCATTCTGGCACTCCCCTTGCTATTGCCTGACTGTACCcaca                                               <  2:447874/140‑1 (MQ=255)                                                                   gTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATACCTTCATATTCAATTAGTTAAATAACTAAATCCAATAATCTCATTCTGGCACTCCCCTTGCTATTGCCTGACTGTACCCACaa                                              <  1:172576/140‑1 (MQ=255)                                                                   gTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATACCTTCATATTCAATTAGTTAAATAACTAAATCCAATAATCTCATTCTGGCACTCCCCTTGCTATTGCCTGACTGTACCCACaa                                              <  1:215069/140‑1 (MQ=255)                                                                    tGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATACCTTCATATTCAATTAGTTAAATAACTAAATCCAATAATCTCATTCTGGCACTCCCCTTGCTATTGCCTGACTGTACCCACAAc                                             >  1:373355/1‑140 (MQ=255)                                                                       gcAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATACCTTCATATTCAATTAGTTAAATAACTAAATCCAATAATCTCATTCTGGCACTCCCCTTGCTATTGCCTGACTGTACCCACAACGgt                                          <  1:222568/140‑1 (MQ=255)                                                                                     aGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATACCTTCATATTCAATTAGTTAAATAACTAAATCCAATAATCTCATTCTGGCACTCCCCTTGCTATTGCCTGACTGTACCCACaa                                              >  1:39844/1‑122 (MQ=255)                                                                                     aGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATACCTTCATATTCAATTAGTTAAATAACTAAATCCAATAATCTCATTCTGGCACTCCCCTTGCTATTGCCTGACTGTACCCACaa                                              <  2:39844/122‑1 (MQ=255)                                                                                          cTCATTCTCCTTATAAATCCCCATCCAATTTATACCTTCATATTCAATTAGTTAAATAACTAAATCCAATAATCTCATTCTGGCACTCCCCTTGCTATTGCCTGACTGTACCCACAACGGTGTATGCAAGAGGGATaaaa                       >  2:286013/1‑140 (MQ=255)                                                                                                           tCCCCATCCAATTTATACCTTCATATTCAATTAGTTAAATAACTAAATCCAATAATCTCATTCTGGCACTCCCCTTGCTATTGCCTGACTGTACCCACAACGGTGTATGCAAGAGGGATAAAAAATGAAAACAAAATtga      <  1:153577/140‑1 (MQ=255)                                                                                                             cccATCCAATTTATACCTTCATATTCAATTAGTTAAATAACTAAATCCAATAATCTCATTCTGGCACTCCCCTTGCTATTGCCTGACTGTACCCACAACGGTGTATGCAAGAGGGATAAAAAATGAAAACAAAATtgatg    >  2:431624/1‑140 (MQ=255)                                                                                                               cATCCAATTTATACCTTCATATTCAATTAGTTAAATAACTAAATCCAATAATCTCATTCTGGCACTCCCCTTGCTATTGCCTGACTGTACCCACAACGGTGTATGCAAGAGGGATAAAAAATGAAAACAAAATTGATGAc  >  1:340777/1‑140 (MQ=255)                                                                                                                           |                                                                                                                                TCCAGGAATACGGTATCGATCCGGCGGATGTATAACACCAAAACTTGCTATGCAGAAATTTGCACAGTGCGCAATTTTCTGCATAGCCGCTCATTCTCCTTATAAATCCCCATCCAATTTATCCCTTCATATTCAATTAGTTAAATAACTAAATCCAATAATCTCATTCTGGCACTCCCCTTGCTATTGCCTGACTGTACCCACAACGGTGTATGCAAGAGGGATAAAAAATGAAAACAAAATTGATGAC  >  NC_000913/2323217‑2323466

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑