Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,857,150	G→T	R419S (CGT→AGT)	yidJ ←	putative sulfatase/phosphatase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,857,150	0	G	T	100.0%	99.5 / NA	30	R419S (CGT→AGT)	yidJ	putative sulfatase/phosphatase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (12/18);  total (12/18)

ATCCAGCGCGGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGTAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCGTCATGCATTTTGCTGCGAACGTCTGCAAAACGGATATCATCGATCAGGTTATGCATTTCATTTGGGTCATTACGGCGATCGTAAAGTTCATCACTGGTGAAGAGGTTGAGTACCAGTTTAAAGTCATCCGTCACCCAGCAACGCACCGGAATAAAAC  >  NC_000913/3857023‑3857275                                                                                                                                |                                                                                                                              aTCCAGCGCGGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGTAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCGTCATGCATTTTGCTGCGAACGTCTGCAAAACTGATATCATCGAt                                                                                                                   <  2:13734/140‑1 (MQ=255)  tCCAGCGCGGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGTAACTGCGGAACGGATCGCGAATTTTGTCCATATAATCCAATAAGGCGTCATGCATTTTGCTGCGAACGTCTGCAAAACTGATATCATCGATc                                                                                                                  <  2:144097/140‑1 (MQ=255)       cgcgGCCGTGCATCTTTACGCCATGGACGCAGACTCCATTGGTAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCGTCATGCATTTTGCTGCGAACGTCTGCAAAACTGATATCATCGATCAGGtt                                                                                                             >  1:291305/1‑140 (MQ=255)       cgcgGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGTAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCAATAGGGCGTCATGCATTTTGCTGCGAACGTCTGCAAAACTGATATCATCGATCAGGtt                                                                                                             >  2:124125/1‑140 (MQ=255)       cgcgGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGTAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCGTCATGCATTTTGCTGCGAACGTCTGCAAAACTGATATCATCGa                                                                                                                    <  2:57463/133‑1 (MQ=255)       cgcgGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGTAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCGTCATGCATTTTGCTGCGAACGTCTGCAAAACTGATATCATCGa                                                                                                                    >  1:57463/1‑133 (MQ=255)       cgcgGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGTAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCGTCATGCATTTTGCTGCGAACGTCTGCAAAACTGATATCATCGATCAGGtt                                                                                                             >  2:141715/1‑140 (MQ=255)         cgGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGTAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCGTCATGCATTTTGCTGCGAACGTCTGCAAAACTGATATCATCGATCAGGTTAt                                                                                                           <  2:323758/140‑1 (MQ=255)              gTGCATCTTTACGCCATGGACGCAGACTCCATTGGTAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCGTCATGCATTTTGCTGCGAACGTCTGCAAAACTGATATCATCGATCAGGTTATGcatt                                                                                                      <  2:291305/140‑1 (MQ=255)                        aCGCCACGGACGCAGACTCCATTGGTAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCGTCATGCATTTTGCTGCGAACGTCTGCAAAACTGATATCATCGATCAGGTTATGCATTTCATTTGGGt                                                                                            <  2:281887/140‑1 (MQ=255)                              cGGACGCAGACTCCATTGGTAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCGTCATGCATTTTGCTGCGAACGTCTGCAAAACTGATATCATCGATCAGGTTATGCATTTCATTTGGGTCATTAc                                                                                      >  1:108222/1‑140 (MQ=255)                              cGGACGCAGACTCCATTGGTAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCGTCATGCATTTTGCTGCGAACGTCTGCAAAACTGATATCATCGATCAGGTTATGCATTTCATTTGGGTCATTAc                                                                                      >  1:449555/1‑140 (MQ=255)                                gACGCAGACTCCATTGGTAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCGTCATGCATTTTGCTGCGAACGTCTGCAAAACTGATATCATCGATCAGGTTATGCATTTCATTTGGGTCATTACgg                                                                                    <  1:120753/140‑1 (MQ=255)                                           cATTGGTAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCGTCATGCATTTTGCTGCGAACGTCTGCAAAACTGATATCATCGATCAGGTTATGCATTTCATTTGGGTCATTACGGCGATCGTAAAg                                                                         <  1:318605/140‑1 (MQ=255)                                           cATTGGTAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCGTCATGCATTTTGCTGCGAACGTCTGCAAAACTGATATCATCGATCAGGTTATGCATTTCATTTGGGTCATTACGGCGATCGTAAAg                                                                         >  1:244483/1‑140 (MQ=255)                                           cATTGGTAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCGTCATGCATTTTGCTGCGAACGTCTGCAAAACTGATATCATCGATCAGGTTATGCATTTCATTTGGGTCATTACGGCGATCGTAAAg                                                                         >  1:221476/1‑140 (MQ=255)                                                  aaCTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCGTCATGCATTTTGCTGCGAACGTCTGCAAAACTGATATCATCGATCAGGTTATGCATTTCATTTGGGTCATTACGGCGATCGTAAAGTtcatca                                                                  <  2:432839/140‑1 (MQ=255)                                                         gAACGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCGTCATGCATTTTGCTGCGAACGTCTGCAAAACTGATATCATCGATCAGGTTATGCATTTCATTTGGGTCATTACGGCGATCGTAAAGTTCATCACTGGTGa                                                           <  2:139905/140‑1 (MQ=255)                                                         gAACGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCGTCATGCATTTTGCTGCGAACGTCTGCAAAACTGATATCATCGATCAGGTTATGCATTTCATTTGGGTCATTACGGCGATCGTAAAGTTCATCACTGGTGa                                                           <  2:445168/140‑1 (MQ=255)                                                          aaCGGATCGCGAATTTTGTCCATGTAATCCAATAGGGCGTCATGCATTTTGCTGCGAACGTCTGCAAAACTGATATCATCGATCAGGTTATGCATTTCATTTGGGTCATTACGGCGATCGTAAAGTTCATCACTGGTGaa                                                          <  1:124125/140‑1 (MQ=255)                                                          aaCGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCGTCATGCATTTTGCTGCGAACGTCTGCAAAACTGATATCATCGATCAGGTTATGCATTTCATTTGGGTCATTACGGCGATCGTAAAGTTCATCCCTGGGGaa                                                          <  2:244483/140‑1 (MQ=255)                                                              gATCGCGAATTTTGTCCATGTAATCCAATAAGGCGTCATGCATTTTGCTGCGAACGTCTGCAAAACTGATATCATCGATCAGGTTATGCATTTCATTTGGGTCATTACGGCGATCGTAAAGTTCATCACTGGTGAAGAgg                                                      <  1:141715/140‑1 (MQ=255)                                                                      aTTTTGTCCATGTAATCCAATAAGGCGTCATGCATTTTGCTGCGAACGTCTGCAAAACTGATATCATCGATCAGGTTATGCATTTCATTTGGGTCATTACGGCGATCGTAAAGTTCATCACTGGTGAAGAGGTTGAGTAc                                              <  2:108222/140‑1 (MQ=255)                                                                      aTTTTGTCCATGTAATCCAATAAGGCGTCATGCATTTTGCTGCGAACGTCTGCAAAACTGATATCATCGATCAGGTTATGCATTTCATTTGGGTCATTACGGCGATCGTAAAGTTCATCACTGGTGAAGAGGTTGAGTAc                                              <  2:85264/140‑1 (MQ=255)                                                                      aTTTTGTCCATGTAATCCAATAAGGCGTCATGCATTTTGCTGCGAACGTCTGCAAAACTGATATCATCGATCAGGTTATGCATTTCATTTGGGTCATTACGGCGATCGTAAAGTTCATCACTGGTGAAGAGGTTGAGTAc                                              <  2:401069/140‑1 (MQ=255)                                                                                    aTCCAATAAGGCGTCATGCATTTTGCTGCGAACGTCTGCAAAACTGATATCATCGATCAGGTTATGCATTTCATTTGGGTCATTACGGCGATCGTAAAGTTCATCACTGGTGAAGAGGTTGAGTACCAGTTTAAAGTCAt                                >  1:25943/1‑140 (MQ=255)                                                                                          tAAGGCGTCATGCATTTTGCTGCGAACGTCTGCAAAACTGATATCATCGATCAGGTTATGCATTTCATTTGGGTCATTACGGCGATCGTAAAGTTCATCACTGGTGAAGAGGTTGAGTACCAGTTTAAAGACATCCGTCa                          >  2:331718/1‑140 (MQ=255)                                                                                                   aTGCATTTTGCTGCGAACGTCTGCAAAACTGATATCATCGATCAGGTTATGCATTTCATTTGGGTCATTACGGCGATCGTAAAGTTCATCACTGGTGAAGAGGTTGAGTACCAGTTTAAAGTCATCCGTCACCCAGCAAc                 >  1:77665/1‑140 (MQ=255)                                                                                                                  aaCGTCTGCAAAACTGATATCATCGATCAGGTTATGCATTTCATTTGGGTCATTACGGCGATCGTAAAGTTCATCACTGGTGAAGAGGTTGAGTACCAGTTTAAAGTCATCCGTCACCCAGCAACGCAc             >  1:244231/1‑129 (MQ=255)                                                                                                                  aaCGTCTGCAAAACTGATATCATCGATCAGGTTATGCATTTCATTTGGGTCATTACGGCGATCGTAAAGTTCATCACTGGTGAAGAGGTTGAGTACCAGTTTAAAGTCATCCGTCACCCAGCAACGCAc             <  2:244231/129‑1 (MQ=255)                                                                                                                  aaCGTCTGCAAAACTGATATCATCGATCAGGTTATGCATTTCATTTGGGTCATTACGGCGATCGTAAAGTTCATCACTGGTGAAGAGGTTGAGTACCAGTTTAAAGTCATCCGTCACCCAGCAACGCACCGGAATAAAAc  <  2:219873/140‑1 (MQ=255)                                                                                                                                |                                                                                                                              ATCCAGCGCGGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGTAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCGTCATGCATTTTGCTGCGAACGTCTGCAAAACGGATATCATCGATCAGGTTATGCATTTCATTTGGGTCATTACGGCGATCGTAAAGTTCATCACTGGTGAAGAGGTTGAGTACCAGTTTAAAGTCATCCGTCACCCAGCAACGCACCGGAATAAAAC  >  NC_000913/3857023‑3857275

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑