Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,224,881	G→T	E352* (GAA→TAA)	metH →	cobalamin‑dependent methionine synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,224,881	0	G	T	100.0%	109.7 / NA	33	E352* (GAA→TAA)	metH	cobalamin‑dependent methionine synthase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (14/19);  total (14/19)

GCGGCTGCTGTGGCACCACGCCACAACATATTGCAGCGATGAGTCGTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTGAACATTGGCGAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCGAGGCGCTGGATGTCGCGCGTCAACAGGTGGAAAACGGCGCGCAGA  >  NC_000913/4224750‑4225016                                                                                                                                    |                                                                                                                                        gcggcTGCTGTGGCACCACGCCACAACATATTGCAGCGATGAGTCGTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTGAACATTGGCTAAGATAGc                                                                                                                                 <  2:226041/140‑1 (MQ=255)                     ccACAACATATTGCAGCGATGAGTCGTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTGAACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGa                                                                                                             >  2:352239/1‑140 (MQ=255)                      cacaACATATTGCAGCGATGAGTCGTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTGAACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGaa                                                                                                            <  2:90328/140‑1 (MQ=255)                      cacaACATATTGCAGCGATGAGTCGTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTGAACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGaa                                                                                                            <  2:68526/140‑1 (MQ=255)                       acaacaTATTGCAGCGATGAGTCGTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGTAAATTCCCGTAGCCTGCCGTTTCTCCGGCCTGGAGCCGCTGAACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGaaa                                                                                                           >  2:367059/1‑139 (MQ=255)                            atatTGCAGCGATGAGTCGTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTGAACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCAcc                                                                                                      <  2:20668/140‑1 (MQ=255)                                tGCAGCGATGAGTCGTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTGAACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACg                                                                                                  >  2:351039/1‑140 (MQ=255)                                tGCAGCGATGAGTCGTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTGAACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACg                                                                                                  >  2:385006/1‑140 (MQ=255)                                  cAGCGATGAGTCGTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTGAACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGCGAACGCACCAACGTc                                                                                                >  1:61709/1‑140 (MQ=255)                                       aTGAGTCGTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTGAACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCgg                                                                                           >  1:201377/1‑140 (MQ=255)                                       aTGAGTCGTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTGAACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCgg                                                                                           >  2:13916/1‑140 (MQ=255)                                          aGTCGTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTGAACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTc                                                                                        >  2:462637/1‑140 (MQ=255)                                               tGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTGAACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTa                                                                                   >  1:394004/1‑140 (MQ=255)                                                       aaGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTGAACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAg                                                                           <  1:280190/140‑1 (MQ=255)                                                          gATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTGAACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAgcgc                                                                        <  1:352239/140‑1 (MQ=255)                                                          gATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTGAACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAgcgc                                                                        <  2:122795/140‑1 (MQ=255)                                                          gATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTGAACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAgcgc                                                                        <  2:52574/140‑1 (MQ=255)                                                            ttAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTGAACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGACACCGGTTACGCTAAGTTCAAGCGCCt                                                                      >  2:286454/1‑140 (MQ=255)                                                              aGCGCCGCGCAAACTGCCGGAAGTTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTGAACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGa                                                                    >  1:267629/1‑140 (MQ=255)                                                              aGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTGAACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCa                                                                             >  1:227110/1‑131 (MQ=255)                                                              aGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTGAACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCa                                                                             <  2:227110/131‑1 (MQ=255)                                                              aGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTGAACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGa                                                                    >  1:345367/1‑140 (MQ=255)                                                              aGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTGAACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGa                                                                    >  2:277276/1‑140 (MQ=255)                                                                 gccgcGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTGAACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCa                                                                 <  2:153011/140‑1 (MQ=255)                                                                    gcgcAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTGAACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAaag                                                              <  2:175224/140‑1 (MQ=255)                                                                    gcgcAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTGAACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAaag                                                              <  1:277276/140‑1 (MQ=255)                                                                                   aaTTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTGAACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCg                                               <  1:13916/140‑1 (MQ=255)                                                                                                           cGGCCTGGAGCCGCTGAACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACGGCGAGGCGCTGGATGTCGCGCGTCAAc                       <  1:405594/140‑1 (MQ=255)                                                                                                           cGGCCTGGAGCCGCTGAACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCGAGGCGCTGGATGTCGCGCGTCAAc                       <  1:156597/140‑1 (MQ=255)                                                                                                                       gCTGAACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCGAGGCGCTGGATGTCGCGCGTCAACAGGTGGAAAACg           >  2:451501/1‑140 (MQ=255)                                                                                                                        cTGAACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCGAGGCGCTGGATGTCGCGCGTCAAc                       >  2:26892/1‑127 (MQ=255)                                                                                                                        cTGAACATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCGAGGCGCTGGATGTCGCGCGTCAAc                       <  1:26892/127‑1 (MQ=255)                                                                                                                            aCATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCGAGGCGCTGGATGTCGCGCGTCAACAGGTGGAAAACGgcgcg      <  1:385006/140‑1 (MQ=255)                                                                                                                            aCATTGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCGAGGCGCTGGATGTCGCGCGTCAACAGGTGGAAAACGgcgcg      <  2:394004/140‑1 (MQ=255)                                                                                                                                tGGCTAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCGAGGCGCTGGATGTCGCGCGTCAACAGGTGGAAAACGGCGCGCAGa  <  1:267395/140‑1 (MQ=255)                                                                                                                                    |                                                                                                                                        GCGGCTGCTGTGGCACCACGCCACAACATATTGCAGCGATGAGTCGTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTGAACATTGGCGAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCGAGGCGCTGGATGTCGCGCGTCAACAGGTGGAAAACGGCGCGCAGA  >  NC_000913/4224750‑4225016

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑