Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,316,106	C→A	W371C (TGG→TGT)	phnM ←	RPnTP hydrolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,316,106	0	C	A	100.0%	83.2 / NA	25	W371C (TGG→TGT)	phnM	RPnTP hydrolase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (10/15);  total (10/15)

CGATGCGCCACCAGTAACTGAGTTTGTTCCCGCAGGCGGAGTTCCGCCAGCAGGCTGTCTTTCCCGGAGCCGGACGGCCCCATTAACCAAATCAGTTTTCCCATCATCAGAACACCCTTTTACCCTGACGCCAGACGTGGTCGATATGAATATGATTGTCCTTGCGATGCGCCAGCACCAGGTCGGCGCGTTTGCCCTCGCCAATCACCCCGCGATCCTGGAGATTAAGCGCCTGCGCTGGATTTTTAGTCACCAGCTTCACCGCCT  >  NC_000913/4315976‑4316242                                                                                                                                   |                                                                                                                                         cGATGCGCCACCAGTAACTGAGTTTGTTCCCGCAGGCGGAGTTCCGCCAGCAGGCTGTCTTTCCCGGAGCCGGACGGCCCCATTAACCAAATCAGTTTTCCCATCATCAGAACACCCTTTTACCCTGACGACAGACGTgg                                                                                                                                 <  1:383400/140‑1 (MQ=255)  gATGCGCCACCAGTAACTGAGTTTGTTCCCGCAGGCGGAGTTCCGCCAGCAGGCTGTCTTTCCCGGAGCCGGACGGCCCCATTAACCAAATCAGTTTTCCCATCATCAGAACACCCTTTTACCCTGACGACAGACGTGGt                                                                                                                                <  1:177718/140‑1 (MQ=255)   aTGCGCCACCAGTAACTGAGTTTGTTCCCGCAGGCGGAGTTCCGCCAGCAGGCTGTCTTTCCCGGAGCCGGACGGCCCCATTAACCAAATCAGTTTTCCCATCATCAGAACACCCTTTTACCCTGACGACAGACGTGGTc                                                                                                                               <  1:368250/140‑1 (MQ=255)      cgcCACCAGTAACTGAGTTTGTTCCCGCAGGCGGAGTTCCGCCAGCAGGCTGTCTTTCCCGGAGCCGGACGGCCCCATTAACCAAATCAGTTTTCCCATCATCAGAACACCCTTTTACCCTGACGACAGACGTGGTCGat                                                                                                                            <  1:202314/140‑1 (MQ=255)      cgcCACCAGTAACTGAGTTTGTTCCCGCAGGCGGAGTTCCGCCAGCAGGCTGTCTTTCCCGGAGCCGGACGGCCCCATTAACCAAATCAGTTTTCCCATCATCAGAACACCCTTTTACCCTGACGACAGACGTGGTCGat                                                                                                                            >  1:56663/1‑140 (MQ=255)      cgcCACCAGTAACTGAGTTTGTTCCCGCAGGCGGAGTTCCGCCAGCAGGCTGTCTTTCCCGGAGCCGGACGGCCCCATTAACCAAATCAGTTTTCCCATCATCAGAACACCCTTTTACCCTGACGACAGACGTGGTCGat                                                                                                                            <  1:448870/140‑1 (MQ=255)      cgcCACCAGTAACTGAGTTTGTTCCCGCAGGCGGAGTTCCACCAGCAGGCTGTCTTTCCCGGAGCCGGACGGCCCCATTAACCAAATCAGTTTTCCCATCATCAGAACACCCTTTTACCCTGACGACAGACGTGGTCGat                                                                                                                            <  2:416022/140‑1 (MQ=255)                              ccGCAGGCGGAGTTCCGCCAGCAGGCTGTCTTTCCCGGAGCCGGACGGCCCCATTAACCAAATCAGTTTTCCCATCATCAGAACACCCTTTTACCCTGACGACAGACGTGGTCGATATGAATATGATTGTCCTTGCGATg                                                                                                    <  1:153109/140‑1 (MQ=255)                                                        tGTCTTTCCCGGAGCCGGACGGCCCCATTAACCAAATCAGTTTTCCCATCATCAGAACACCCTTTTACCCTGACGACAGACGTGGTCGATATGAATATGATTGTCCTTGCGATGCGCCAGCACCAGGTCGGCGCGTTTg                                                                           >  1:156128/1‑139 (MQ=255)                                                        tGTCTTTCCCGGAGCCGGACGGCCCCATTAACCAAATCAGTTTTCCCATCATCAGAACACCCTTTTACCCTGACGACAGACGTGGTCGATATGAATATGATTGTCCTTGCGATGCGCCAGCACCAGGTCGGCGCGTTTg                                                                           <  2:156128/139‑1 (MQ=255)                                                         gTCTTTCCCGGAGCCGGACGGCCCCATTAACCAAATCAGTTTTCCCATCATCAGAACACCCTTTTACCCTGACGACAGACGTGGTCGATATGAATATGATTGTCCTTGCGATGCGCCAGCACCAGGTCGGCGCGTTTGcc                                                                         <  2:243579/140‑1 (MQ=255)                                                                         gACGGCCCCATTAACCAAATCAGTTTTCCCATCATCAGAACACCCTTTTACCCTGACGACAGACGTGGTCGATATGAATATGATTGTCCTTGCGATGCGCCAGCACCAGGTCGGCGCGTTTGCCCTCGCCAATCACCCcg                                                         <  2:195925/140‑1 (MQ=255)                                                                         gACGGCCCCATTAACCAAATCAGTTTTCCCATCATCAGAACACCCTTTTACCCTGACGACAGACGTGGTCGATATGAATATGATTGTCCTTGCGATGCGCCAGCACCAGGTCGGCGCGTTTGCCCTCGCCAATCACCCcg                                                         <  2:221998/140‑1 (MQ=255)                                                                                ccATTAACCAAATCAGTTTTCCCATCATCAGAACACCCTTTTACCCTGACGACAGACGTGGTCGATATGAATATGATTGTCCTTGCGATGCGCCAGCACCAGGTCGGCGCGTTTGCCCTCGCCAATCACCCCGCGATCCt                                                  <  1:74549/140‑1 (MQ=255)                                                                                        cAAATCAGTTTTCCCATCATCAGAACACCCTTTTACCCTGACGACAGACGTGGTCGATATGAATATGATTGTCCTTGCGATGCGCCAGCACCAGGTCGGCGCGTTTGCCCTCGCCAATCACCCcg                                                         <  1:314565/125‑1 (MQ=255)                                                                                        cAAATCAGTTTTCCCATCATCAGAACACCCTTTTACCCTGACGACAGACGTGGTCGATATGAATATGATTGTCCTTGCGATGCGCCAGCACCAGGTCGGCGCGTTTGCCCTCGCCAATCACCCcg                                                         >  2:314565/1‑125 (MQ=255)                                                                                          aaTCAGTTTTCCCATCATCAGAACACCCTTTTACCCTGACGACAGACGTGGTCGATATGAATATGATTGTCCTTGCGATGCGCCAGCACCAGGTCGGCGCGTTTGCCCTCGCCAATCACCCCGCGATCCTGGAGATTAAg                                        >  1:232313/1‑140 (MQ=255)                                                                                           aTCAGTTTTCCCATCATCAGAACACCCTTTTACCCTGACGACAGACGTGGTCGATATGAATATGATTGTCCTTGCGATGCGCCAGCACCAGGTCGGCGCGTTTGCCCTCGCCAATCACCCCGCGATCCTGGAGATTAAgc                                       >  1:369883/1‑140 (MQ=255)                                                                                                       atcatcAGAACACCCTTTTACCCTGACGACAGACGTGGTCGATATGAATATGATTGTCCTTGCGATGCGCCAGCACCAGGTCGGCGCGTTTGCCCTCGCCAATCACCCCGCGATCCTGGAGATTAAGCGCCTGCGCTGGa                           >  2:168634/1‑140 (MQ=255)                                                                                                               aaCACCCTTTTACCCTGACGACAGACGTGGTCGATATGAATATGATTGTCCTTGCGATGCGCCAGCACCAGGTCGGCGCGTTTGCCCTCGCCAATCACCCCGCGATCCTGGAGATTAAGCGCCTGCGCTGGATTTTTAGt                   >  1:183832/1‑140 (MQ=255)                                                                                                                    ccTTTTACCCTGACGACAGACGTGGTCGATATGAATATGATTGTCCTTGCGATGCGCCAGCACCAGGTCGGCGCGTTTGCCCTCGCCAATCACCCCGCGATCCTGGAGATTAAGCGCCTGCGCTGGATTTTTAGTCACCa              >  1:210761/1‑140 (MQ=255)                                                                                                                      ttttACCCTGACGACAGACGTGGTCGATATGAATATGATTGTCCTTGCGATGCGCCAGCACCAGGTCGGCGCGTTTGCCCTCGCCAATCACCCCGCGATCCTGGAGATTAAGCGCCTGCGCTg                             >  1:8576/1‑123 (MQ=255)                                                                                                                      ttttACCCTGACGACAGACGTGGTCGATATGAATATGATTGTCCTTGCGATGCGCCAGCACCAGGTCGGCGCGTTTGCCCTCGCCAATCACCCCGCGATCCTGGAGATTAAGCGCCTGCGCTg                             <  2:8576/123‑1 (MQ=255)                                                                                                                          aCCCTGACGACAGACGTGGTCGATATGAATATGATTGTCCTTGCGATGCGCCAGCACCAGGTCGGCGCGTTTGCCCTCGCCAATCACCCCGCGATCCTGGAGATTAAGCGCCTGCGCTGGATTTTTAGTCACCAGCTTCa        <  2:232313/140‑1 (MQ=255)                                                                                                                                aCGACAGACGTGGTCGATATGAATATGATTGTCCTTGCGATGCGCCAGCACCAGGTCGGCGCGTTTGCCCTCGCCAATCACCCCGCGATCCTGGAGATTAAGCGCCTGCGCTGGATTTTTAGTCACCAGCTTCACCGCCt  >  2:311949/1‑140 (MQ=255)                                                                                                                                   |                                                                                                                                         CGATGCGCCACCAGTAACTGAGTTTGTTCCCGCAGGCGGAGTTCCGCCAGCAGGCTGTCTTTCCCGGAGCCGGACGGCCCCATTAACCAAATCAGTTTTCCCATCATCAGAACACCCTTTTACCCTGACGCCAGACGTGGTCGATATGAATATGATTGTCCTTGCGATGCGCCAGCACCAGGTCGGCGCGTTTGCCCTCGCCAATCACCCCGCGATCCTGGAGATTAAGCGCCTGCGCTGGATTTTTAGTCACCAGCTTCACCGCCT  >  NC_000913/4315976‑4316242

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑