Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,405,335	G→T	D217Y (GAT→TAT)	purA →	adenylosuccinate synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,405,335	0	G	T	100.0%	140.6 / NA	41	D217Y (GAT→TAT)	purA	adenylosuccinate synthetase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (19/22);  total (19/22)

TCCAGTTGGTTAACTACTACAAAGCTGAAGCGGTTGATTACCAGAAAGTTCTGGATGATACGATGGCTGTTGCCGACATCCTGACTTCTATGGTGGTTGACGTTTCTGACCTGCTCGACCAGGCGCGTCAGCGTGGCGATTTCGTCATGTTTGAAGGTGCGCAGGGTACGCTGCTGGATATCGACCACGGTACTTATCCGTACGTAACTTCTTCCAACACCACTGCTGGTGGCGTGGCGACCGGTTCCGGCCTGGGCCCGCGTTATGTTGATT  >  NC_000913/4405198‑4405470                                                                                                                                          |                                                                                                                                        tCCAGTTGGTTAACTACTACAAAGCTGAAGCGGTTGATTACCAGAAAGTTCTGGATGATACGATGGCTGTTGCCGACATCCTGACTTCTATGGTGGTTGACGTTTCTGACCTGCTCGACCAGGCGCGTCAGCGTGGCTAt                                                                                                                                       >  1:385185/1‑140 (MQ=255)    aGTTGGTTAACTACTACAAAGCTGAAGCGGTTGATTACCAGAAAGTTCTGGATGATACGATGGCTGTTGCCGACATCCTGACTTCTATGGTGGTTGACGTTTCTGACCTGCTCGACCAGGCGCGTCAGCGTGGCTATTTc                                                                                                                                    >  1:283864/1‑140 (MQ=255)       tGGTTAACTACTACAAAGCTGAAGCGGTTGATTACCAGAAAGTTCTGGATGATACGATGGCTGTTGCCGACATCCTGACTTCTATGGTGGTTGACGTTTCTGACCTGCTCGACCAGGCGCGTCAGCGTGGCTATTTCGTc                                                                                                                                 <  1:421831/140‑1 (MQ=255)       tGGTTAACTACTACAAAGCTGAAGCGGTTGATTACCAGAAAGTTCTGGATGATACGATGGCTGTTGCCGACATCCTGACTTCTATGGTGGTTGACGTTTCTGACCTGCTCGACCAGGCGCGTCAGCGTGGCTATTTCGTc                                                                                                                                 <  2:335620/140‑1 (MQ=255)             actactACAAAGCTGAAGCGGTTGATTACCAGAAAGTTCTGGATGATACGATGGCTGTTGCCGACATCCTGACTTCTATGGTGGTTGACGTTTCTGACCTGCTCGACCAGGCGCGTCAGCGTGGCTATTTCGTCATGttt                                                                                                                           >  2:417641/1‑140 (MQ=255)                actacAAAGCTGAAGCGGTTGATTACCAGAAAGTTCTGGATGATACGATGGCTGTTGCCGACATCCTGACTTCTATGGTGGTTGACGTTTCTGACCTGCTCGACCAGGCGCGTCAGCGTGGCTATTTCGTCATGTTTGaa                                                                                                                        <  2:326984/140‑1 (MQ=255)                       aGCTGAAGCGGTTGATTACCAGAAAGTTCTGGATGATACGATGGCTGTTGCCGACATCCTGACTTCTATGGTGGTTGACGTTTCTGACCTGCTCGACCAGGCGCGTCAGCGTGGCTATTTCGTCATGTTTGAAGGTgcgc                                                                                                                 >  1:141260/1‑140 (MQ=255)                        gCTGAAGCGGTTGATTACCAGAAAGTTCTGGATGATACGATGGCTGTTGCCGACATCCTGACTTCTATGGTGGTTGACGTTTCTGACCTGCTCGACCAGGCGCGTCAGCGTGGCTATTTCGTCATGTTTGAAGGTGCGCa                                                                                                                <  1:417641/140‑1 (MQ=255)                            aaGCGGTTGATTACCAGAAAGTTCTGGATGATACGATGGCTGTTGCCGACATCCTGACTTCTATGGTGGTTGACGTTTCTGACCTGCTCGACCAGGCGCGTCAGCGTGGCTATTTCGTCATGTTTGAAGGTGCGCAGGGt                                                                                                            >  1:408359/1‑140 (MQ=255)                                 gTTGATTACCAGAAAGTTCTGGATGATACGATGGCTGTTGCCGACATCCTGACTTCTATGGTGGTTGACGTTTCTGACCTGCTCGACCAGGCGCGTCAGCGTGGCTATTTCGTCATGTTTGAAGGTGCGCAGGGTACg                                                                                                         >  2:183798/1‑138 (MQ=255)                                 gTTGATTACCAGAAAGTTCTGGATGATACGATGGCTGTTGCCGACATCCTGACTTCTATGGTGGTTGACGTTTCTGACCTGCTCGACCAGGCGCGTCAGCGTGGCTATTTCGTCATGTTTGAAGGTGCGCAAGGTACg                                                                                                         <  1:183798/138‑1 (MQ=255)                                           aGAAAGTTCTGGATGATACGATGGCTGTTGCCGACATCCTGACTTCTATGGTGGTTGACGTTTCTGACCTGCTCGACCAGGCGCGTCAGCGTGGCTATTTCGTCATGTTTGAAGGTGCGCAGGGTACGCTGCTGGATATc                                                                                             <  2:141260/140‑1 (MQ=255)                                            gAAAGTTCTGGATGATACGATGGCTGTTGCCGACATCCTGACTTCTATGGTGGTTGACGTTTCTGACCTGCTCGACCAGGCGCGTCAGCGTGGCTATTTCGTCATGTTTGAAGGTGCGCAGGGTACGCTGCTGGATATCg                                                                                            >  2:79164/1‑140 (MQ=255)                                             aaaGTTCTGGATGATACGATGGCTGTTGCCGACATCCTGACTTCTATGGTGGTTGACGTTTCTGACCTGCTCGACCAGGCGCGTCAGCGTGGCTATTTCGTCATGTTTGAAGGTGCGCAGGGTACGCTGCTGGATATCGa                                                                                           <  2:284477/140‑1 (MQ=255)                                              aaGTTCTGGATGATACGATGGCTGTTGCCGACATCCTGACTTCTATGGTGGTTGACGTTTCTGACCTGCTCGACCAGGCGCGTCAGCGTGGCTATTTCGTCATGTTTGAAGGTGCGCAGGGTACGCTGCTGGATATCGAc                                                                                          <  1:138251/140‑1 (MQ=255)                                              aaGTTCTGGATGATACGATGGCTGTTGCCGACATCCTGACTTCTATGGTGGTTGACGTTTCTGACCTGCTCGACCAGGCGCGTCAGCGTGGCTATTTCGTCATGTTTGAAGGTGCGCAGGGTACGCTGCTGGATATCGAc                                                                                          <  1:13843/140‑1 (MQ=255)                                                gTTCTGGATGATACGATGGCTGTTGCCGACATCCTGACTTCTATGGTGGTTGACGTTTCTGACCTGCTCGACCAGGCGCGTCAGCGTGGCTATTTCGTCATGTTTGAAGGTGCGCAGGGTACGCTGCTGGATATCGACCa                                                                                        <  1:79164/140‑1 (MQ=255)                                                     ggATGATACGATGGCTGTTGCCGACATCCTGACTTCTATGGTGGTTGACGTTTCTGACCTGCTCGACCAGGCGCGTCAGCGTGGCTATTTCGTCATGTTTGAAGGTGCGCAGGGTACGCTGCTGGATATCGACCACGGTa                                                                                   <  1:190678/140‑1 (MQ=255)                                                           tACGATGGCTGTTGCCGACATCCTGACTTCTATGGTGGTTGACGTTTCTGACCTGCTCGACCAGGCGCGTCAGCGTGGCTATTTCGTCATGTTTGAAGGTGCGCAGGGTACGCTGCTGGATATCGACCACGGTACTTATc                                                                             >  1:4014/1‑140 (MQ=255)                                                           tACGATGGCTGTTGCCGACATCCTGACTTCTATGGTGGTTGACGTTTCTGACCTGCTCGACCAGGCGCGTCAGCGTGGCTATTTCGTCATGTTTGAAGGTGCGCAGGGTACGCTGCTGGATATCGACCACGGTACTTATc                                                                             >  2:220371/1‑140 (MQ=255)                                                           tACGATGGCTGTTGACGACATCCTGACTTCTATGGTGGTTGACGTTTCTGACCTGCTCGACCAGGCGCGTCAGCGTGGCTATTTCGTCATGTTTGAAGGTGCGCAGGGTACGCTGCTGGATATCGAc                                                                                          <  2:87672/127‑1 (MQ=255)                                                           tACGATGGCTGTTGACGACATCCTGACTTCTATGGTGGTTGACGTTTCTGACCTGCTCGACCAGGCGCGTCAGCGTGGCTATTTCGTCATGTTTGAAGGTGCGCAGGGTACGCTGCTGGATATCGAc                                                                                          >  1:87672/1‑127 (MQ=255)                                                                      ttGCCGACATCCTGACTTCTATGGTGGTTGACGTTTCTGACCTGCTCGACCAGGCGCGTCAGCGTGGCTATTTCGTCATGTTTGAAGGTGCGCAGGGTACGCTGCTGGATATCGACCACGGTACTTATCCGTACGTAAct                                                                  <  2:385185/140‑1 (MQ=255)                                                                                   gACTTCTATGGTGGTTGACGTTTCTGACCTGCTCGACCAGGCGCGTCAGCGTGGCTATTTCGTCATGTTTGAAGGTGCGCAGGGTACGCTGCTGGATATCGACCACGGTACTTATCCGTACGTAACTTCTTCCAAcacca                                                     <  2:283864/140‑1 (MQ=255)                                                                                         tATGGTGGTTGACGTTTCTGACCTGCTCGACCAGGCGCGTCAGCGTGGCTATTTCGTCATGTTTGAAGGTGCGCAGGGTACGCTGCTGGATATCGACCACGGTACTTATCCGTACGTAACTTCTTCCAACACCActgctg                                               >  2:240546/1‑140 (MQ=255)                                                                                                gtTGACGTTTCTGACCTGCTCGACCAGGCGCGTCAGCGTGGCTATTTCGTCATGTTTGAAGGTGCGCAGGGTACGCTGCTGGATATCGACCACGGTACTTATCCGTACGTAACTTCTTCCAACACCACTGCTGgtggcgt                                        >  1:192201/1‑140 (MQ=255)                                                                                                gtTGACGTTTCTGACCTGCTCGACCAGGCGCGTCAGCGTGGCTATTTCGTCATGTTTGAAGGTGCGCAGGGTACGCTGCTGGATATCGACCACGGTACTTATCCGTACGTAACTTCTTCCAACACCACTGCTGgtggcgt                                        >  1:419698/1‑140 (MQ=255)                                                                                                gtTGACGTTTCTGACCTGCTCGACCAGGCGCGTCAGCGTGGCTATTTCGTCATGTTTGAAGGTGCGCAGGGTACGCTGCTGGATATCGACCACGGTACTTATCCGTACGTAACTTCTTCCAACACCACTGCTGgtggcgt                                        >  2:283542/1‑140 (MQ=255)                                                                                                gtTGACGTTTCTGACCTGCTCGACCAGGCGCGTCAGCGTGGCTATTTCGTCATGTTTGAAGGTGCGCAGGGTACGCTGCTGGATATCGACCACGGTACTTATCCGTACGTAACTTCTTCCAACACCACTGCTGgtggcgt                                        >  2:382458/1‑140 (MQ=255)                                                                                                      gTTTGTGACCTGCTCGACCAGGCGCGTCAGCGTGGCTATTTCGTCATGTTTGAAGGTGCGCAGGGTACGCTGCTGGATATCGACCACGGTACTTATCCGTACGTAACTTCTTCCAACACCACTGCTGGTGGCGTGGCGAc                                  <  2:230172/140‑1 (MQ=255)                                                                                                      gTTTCTGACCTGCTCGACCAGGCGCGTCAGCGTGGCTATTTCGTCATGTTTGAAGGTGCGCAGGGTACGCTGCTGGATATCGACCACGGTACTTATCCGTACGTAACTTCTTCCAACACCACTGCTGGTGGCGTGGCGAc                                  <  1:191000/140‑1 (MQ=255)                                                                                                      gTTTCTGACCTGCTCGACCAGGCGCGTCAGCGTGGCTATTTCGTCATGTTTGAAGGTGCGCAGGGTACGCTGCTGGATATCGACCACGGTACTTATCCGTACGTAACTTCTTCCAACACCACTGCTGGTGGCGTGGCGAc                                  <  1:283542/140‑1 (MQ=255)                                                                                                                  cTCGACCAGGCGCGTCAGCGTGGCTATTTCGTCATGTTTGAAGGTGCGCAGGGTACGCTGCTGGATATCGACCACGGTACTTATCCGTACGTAACTTCTTCCAACACCACTGCTGGTGGCGTGGCGACCGGTTCCGGCCt                      >  1:297680/1‑140 (MQ=255)                                                                                                                  cTCGACCAGGCGCGTCAGCGTGGCTATTTCGTCATGTTTGAAGGTGCGCAGGGTACGCTGCTGGATATCGACCACGGTACTTATCCGTACGTAACTTCTTCCAACACCACTGCTGGTGGCGTGGCGACCGGTTCCGGCCt                      >  2:113872/1‑140 (MQ=255)                                                                                                                      aCCAGGCGCGTCAGCGTGGCTATTTCGTCATGTTTGAAGGTGCGCAGGGTACGCTGCTGGATATCGACCACGGTACTTATCCGTACGTAACTTCTTCCAACACCACTGCTGGTGGCGTGGCGACCGGTTCCGGCCTGGGc                  <  2:192201/140‑1 (MQ=255)                                                                                                                      aCCAGGCGCGTCAGCGTGGCTATTTCGTCATGTTTGAAGGTGCGCAGGGTACGCTGCTGGATATCGACCACGGTACTTATCCGTACGTAACTTCTTCCAACACCACTGCTGGTGGCGTGGCGACCGGTTCCGGCCTGGGc                  <  2:186886/140‑1 (MQ=255)                                                                                                                        cAGGCGCGTCAGCGTGGCTATTTCGTCATGTTTGAAGGTGCGCAGGGTACGCTGCTGGATATCGACCACGGTACTTATCCGTACGTAACTTCTTCCAACACCACTGCTGGTGGCGTGGCGACCGGTTCCGGCCTGGGccc                <  1:240546/140‑1 (MQ=255)                                                                                                                           gcgcGTCAGCGTGGCTATTTCGTCATGTTTGAAGGTGCGCAGGGTACGCTGCTGGATATCGACCACGGTACTTATCCGTACGTAACTTCTTCCAACACCACTGCTGGTGGCGTGGCGACCGGTTCCGGCCTGGGCCcgcg             <  2:4014/140‑1 (MQ=255)                                                                                                                                tCAGCGTGGCTATTTCGTCATGTTTGAAGGTGCGCAGGGTACGCTGCTGGATATCGACCACGGTACTTATCCGTACGTAACTTCTTCCAACACCACTGCTGGTGGCGTGGCGACCGGTTCCGGCCTGGGCCCGCGTTATg        >  2:307776/1‑140 (MQ=255)                                                                                                                                 cAGCGTGGCTATTTCGTCATGTTTGAAGGTGCGCAGGGTACGCTGCTGGATATCGACCACGGTACTTATCCGTACGTAACTTCTTCCAACACCACTGCTGGTGGCGTGGCGACCGGTTCCGGCCTGGGCCCGCGTTATGt       >  1:411338/1‑140 (MQ=255)                                                                                                                                      tGGCTATTTCGTCATGTTTGAAGGTGCGCAGGGTACGCTGCTGGATATCGACCACGGTACTTATCCGTACGTAACTTCTTCCAACACCACTGCTGGTGGCGTGGCGACCGGTTCCGGCCTGGGCCCGCGTTATGTTGAtt  <  2:411338/140‑1 (MQ=255)                                                                                                                                          |                                                                                                                                        TCCAGTTGGTTAACTACTACAAAGCTGAAGCGGTTGATTACCAGAAAGTTCTGGATGATACGATGGCTGTTGCCGACATCCTGACTTCTATGGTGGTTGACGTTTCTGACCTGCTCGACCAGGCGCGTCAGCGTGGCGATTTCGTCATGTTTGAAGGTGCGCAGGGTACGCTGCTGGATATCGACCACGGTACTTATCCGTACGTAACTTCTTCCAACACCACTGCTGGTGGCGTGGCGACCGGTTCCGGCCTGGGCCCGCGTTATGTTGATT  >  NC_000913/4405198‑4405470

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑