Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	963,152	C→A	G386G (GGC→GGA)	rpsA →	30S ribosomal subunit protein S1

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	963,152	0	C	A	100.0%	93.4 / NA	28	G386G (GGC→GGA)	rpsA	30S ribosomal subunit protein S1
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (16/12);  total (16/12)

ATCTCCCTGGGTCTGAAACAGTGCAAAGCTAACCCGTGGCAGCAGTTCGCGGAAACCCACAACAAGGGCGACCGTGTTGAAGGTAAAATCAAGTCTATCACTGACTTCGGTATCTTCATCGGCTTGGACGGCGGCATCGACGGCCTGGTTCACCTGTCTGACATCTCCTGGAACGTTGCAGGCGAAGAAGCAGTTCGTGAATACAAAAAAGGCGACGAAATCGCTGCAGTTGTTCTGCAGGTTGACGCAGAACGTGAACGTATCTCCCT  >  NC_000913/963018‑963286                                                                                                                                       |                                                                                                                                       aTCTCCCTGGGTCTGAAACAGTGCAAAGCTAACCCGTGGCAGCAGTTCGCGGAAACCCACAACAAGGGCGACCGTGTTGAAGGTAAAATCAAGTCTATCACTGACTTCGGTATCTTCATCGGCTTGGACGGCGGAATCGa                                                                                                                                   <  2:4156/140‑1 (MQ=255)   ctcCCTGGGTCTGAAACAGTGCAAAGCTAACCCGTGGCAGCAGTTCGCGGAAACCCACAACAAGGGCGACCGTGTTGAAGGTAAAATCAAGTCTATCACTGACTTCGGTATCTTCATCGGCTTGGACGGCGGAATCGACg                                                                                                                                 >  2:289880/1‑140 (MQ=255)     cccTGGGTCTGAAACAGTGCAAAGCTAACCCGTGGCAGCAGTTCGCGGAAACCCACAACAAGGGCGACCGTGTTGAAGGTAAAATCAAGTCTATCACTGACTTCGGTATCTTCATCGGCTTGGACGGCGGAATCGACGGc                                                                                                                               >  1:146265/1‑140 (MQ=255)          ggTCTGAAACAGTGCAAAGCTAACCCGTGGCAGCAGTTCGCGGAAACCCACAACAAGGGCGACCGTGTTGAAGGTAAAATCAAGTCTATCACTGACTTCGGTATCTTCATCGGCTTGGACGGCGGAATCGACGGCCTGGt                                                                                                                          >  2:250444/1‑140 (MQ=255)                aaaCAGTGCAAAGCTAACCCGTGGCAGCAGTTCGCGGAAACCCACAACAAGGGCGACCGTGTTGAAGGTAAAATCAAGTCTATCACTGACTTCGGTATCTTCATCGGCTTGGACGGCGGAATCGACgg                                                                                                                                >  1:204702/1‑128 (MQ=255)                aaaCAGTGCAAAGCTAACCCGTGGCAGCAGTTCGCGGAAACCCACAACAAGGGCGACCGTGTTGAAGGTAAAATCAAGTCTATCACTGACTTCGGTATCTTCATCGGCTTGGACGGCGGAATCGACgg                                                                                                                                <  2:204702/128‑1 (MQ=255)                     gTGCAAAGCTAACCCGTGGCAGCAGTTCGCGGAAACCCACAACAAGGGCGACCGTGTTGAAGGTAAAATCAAGTCTATCACTGACTTCGGTATCTTCATCGGCTTGGACGGCGGAATCGACGGCCTGGTTCACCTGTCTg                                                                                                               >  1:147875/1‑140 (MQ=255)                      tGCAAAGCTAACCCGTGGCAGCAGTTCGCGGAAACCCACAACAAGGGCGACCGTGTTGAAGGTAAAATCAAGTCTATCACTGACTTCGGTATCTTCATCGGCTTGGACGGCGGAATCGACGGCCTGGTTCACCTGTCTGa                                                                                                              >  1:20244/1‑140 (MQ=255)                      tGCAAAGCTAACCCGTGGCAGCAGTTCGCGGAAACCCACAACAAGGGCGACCGTGTTGAAGGTAAAATCAAGTCTATCACTGACTTCGGTATCTTCATCGGCTTGGACGGCGGAATCGACGGCCTGGTTCACCTGTCTGa                                                                                                              >  2:318717/1‑140 (MQ=255)                       gCACAGCTAACCCGTGGCAGCACTTCGCGGAAACCCACAACAAGGGCGACCGTGTTGAAGGTAAAATCAAGTCTATCACTGACTTCGGTATCTTCATCGGCTTGGACGGCGGAATCGACGGCCTGGTTCACCTGTCTGAc                                                                                                             <  2:462001/140‑1 (MQ=255)                       gCAAAGCTAACCCGTGGCAGCAGTTCGCGGAAACCCACAACAAGGGCGACCGTGTTGAAGGTAAAATCAAGTCTATCACTGACTTCGGTATCTTCATCGGCTTGGACGGCGGAATCGACGGCCTGGTTCACCTGTCTGAc                                                                                                             <  1:250444/140‑1 (MQ=255)                        cAAAGCTAACCCGTGGCAGCAGTTCGCGGAAACCCACAACAAGGGCGACCGTGTTGAAGGTAAAATCAAGTCTATCACTGACTTCGGTATCTTCATCGGCTTGGACGGCGGAATCGACGGCCTGGTTCACCTGTCTGACa                                                                                                            <  1:163439/140‑1 (MQ=255)                               aaCCCGTGGCAGCAGTTCGCGGAAACCCACAACAAGGGCGACCGTGTTGAAGGTAAAATCAAGTCTATCACTGACTTCGGTATCTTCATCGGCTTGGACGGCGGAATCGACGGCCTGGTTCACCTGTCTGACATCTCCTg                                                                                                     >  2:299961/1‑140 (MQ=255)                                       gcagcaGTTCGCGGAAACCCACAACAAGGGCGACCGTGTTGAAGGTAAAATCAAGTCTATCACTGACTTCGGTATCTTCATCGGCTTGGACGGCGGAATCGACGGCCTGGTTCACCTGTCTGACATCTCCTGGAACGTTg                                                                                             >  2:427876/1‑140 (MQ=255)                                       gcagcaGTTCGCGGAAACCCACAACAAGGGCGACCGTGTTGAAGGTAAAATCAAGTCTATCACTGACTTCGGTATCTTCATCGGCTTGGACGGCGGAATCGACGGCCTGGTTCACCTGTCTGACATCTCCTGGAACGTTg                                                                                             >  1:259314/1‑140 (MQ=255)                                       gcagcaGTTCGCGGAAACCCACAACAAGGGCGACCGTGTTGAAGGTAAAATCAAGTCTATCACTGACTTCGGTATCTTCATCGGCTTGGACGGCGGAATCGACGGCCTGGTTCACCTGTCTGACATCTCCTGGAACGTTg                                                                                             >  1:429233/1‑140 (MQ=255)                                                                 aGGGCGACCGTGTTGAAGGTAAAATCAAGTCTATCACTGACTTCGGTATCTTCATCGGCTTGGACGGCGGAATCGACGGCCTGGTTCACCTGTCTGACATCTCCTGGAACGTTGCAGGCGAAGAAGCAGTTCGTGAATAc                                                                   >  1:38460/1‑140 (MQ=255)                                                                  gggCGACCGTGTTGAAGGTAAAATCAAGTCTATCACTGACTTCGGTATCTTCATCGGCTTGGACGGCGGAATCGACGGCCTGGTTCACCTGTCTGACATCTCCTGGAACGTTGCAGGCGAAGAAGCAGTTCGTGAATACa                                                                  <  2:20244/140‑1 (MQ=255)                                                                                  ggTAAAATCAAGTCTATCACTGACTTCGGTATCTTCATCGGCTTGGACGGCGGAATCGACGGCCTGGTTCACCTGTCTGACATCTCCTGGAACGTTGCAGGCGAAGAAGCAGTTCGTGAATACAAAAAAGGCGACGAAAt                                                  <  1:318717/140‑1 (MQ=255)                                                                                     aaaaTCAAGTCTATCACTGACTTCGGTATCTTCATCGGCTTGGACGGCGGAATCGACGGCCTGGTTCACCTGTCTGACATCTCCTGGAACGTTGCAGGCGAAGAAGCAGTTCGTGAATACAAAAAAGGCGACGAAATCGc                                               >  2:24512/1‑140 (MQ=255)                                                                                             gTCTATCACTGACTTCGGTATCTTCATCGGCTTGGACGGCGGAATCGACGGCCTGGTTCACCTGTCTGACATCTCCTGGAACGTTGCAGGCGAAGAAGCAGTTCGTGAATACAAAAAAGGCGACGAAATCGCTGCAgttg                                       <  1:140436/140‑1 (MQ=255)                                                                                                  tCACTGACTTCGGTATCTTCATCGGCTTGGACGGCGGAATCGACGGCCTGGTTCACCTGTCTGACATCTCCTGGAACGTTGCAGGCGAAGAAGCAGTTCGTGAATACAAAAAAGGCGACGAAATCGCTGCAGTTGTTCTg                                  <  1:94228/140‑1 (MQ=255)                                                                                                  tCACTGACTTCGGTATCTTCATCGGCTTGGACGGCGGAATCGACGGCCTGGGTCACCTGTCTGACATCTCCTGGAACGTTGCAGGCGAAGAAGCAGTTCGTGAATACAAAAAAGGCGACGAAATCGCTGCAGTTGTTCTg                                  >  2:245084/1‑140 (MQ=255)                                                                                                      tGACTTCGGTATCTTCATCGGCTTGGACGGCGGAATCGACGGCCTGGTTCACCTGTCTGACATCTCCTGGAACGTTGCAGGCGAAGAAGCAGTTCGTGAATACAAAAAAGGCGACGAAATCGCTGCAGTTGTTCTGCAgg                              <  2:259314/140‑1 (MQ=255)                                                                                                            cGGTATCTTCATCGGCTTGGACGGCGGAATCGACGGCCTGGTTCACCTGTCTGACATCTCCTGGAACGTTGCAGGCGAAGAAGCAGTTCGTGAATACAAAAAAGGCGACGAAATCGCTGCAGTTGTTCTGCAGGTTGACg                        <  2:147875/140‑1 (MQ=255)                                                                                                            cGGTATCTTCATCGGCTTGGACGGCGGAATCGACGGCCTGGTTCACCTGTCTGACATCTCCTGGAACGTTGCAGGCGAAGAAGCAGTTCGTGAATACAAAAAAGGCGACGAAATCGCTGCAGTTGTTCTGCAGGTTGACg                        >  1:399241/1‑140 (MQ=255)                                                                                                                 tCTTCATCGGCTTGGACGGCGGAATCGACGGCCTGGTTCACCTGTCTGACATCTCCTGTAACGTTGCAGGCGAAGAAGCAGTTCGTGAATACAAAAAAGGCGACGAAATCGCTGCAGTTGTTCTGCAGGTTGACGCAgaa                   <  2:329157/140‑1 (MQ=255)                                                                                                                   ttCATCGGCTTGGACGGCGGAATCGACGGCCTGGTTCACCTGTCTGACATCTCCTGGAACGTTGCAGGCGAAGAAGCAGTTCGTGAATACAAAAAAGGCGACGAAATCGCTGCAGTTGTTCTGCAGGTTGACGCAgaacg                 >  2:48340/1‑140 (MQ=255)                                                                                                                          gCTTGGACGGCGGAATCGACGGCCTGGTTCACCTGTCTGACATCTCCTGGAACGTTGCAGGCGAAGAAGCAGTTCGTGAATACAAAAAAGGCGACGAAATCGCTGCAGTTGTTCTGCAGGTTGACGCAgaacgtgaacgt          <  1:24512/140‑1 (MQ=255)                                                                                                                             tGGACGGCGGAATCGACGGCCTGGTTCACCTGTCTGACATCTCCTGGAACGTTGCAGGCGAAGAAGCAGTTCGTGAATACAAAAAAGGCGACGAAATCGCTGCAGTTGTTCTGCAGGTTGACGCAGAACGTGAACGTAtc       >  1:264775/1‑140 (MQ=255)                                                                                                                                  ggcggaATCGACGGCCTGGTTCACCTGTCTGACATCTCCTGGAACGTTGCAGGCGAAGAAGCAGTTCGTGAATACAAAAAAGGCGACGAAATCGCTGCAGTTGTTCTGCAGGTTGACGCAGAACGTGAACGTATCTCCCt  <  2:81546/140‑1 (MQ=255)                                                                                                                                       |                                                                                                                                       ATCTCCCTGGGTCTGAAACAGTGCAAAGCTAACCCGTGGCAGCAGTTCGCGGAAACCCACAACAAGGGCGACCGTGTTGAAGGTAAAATCAAGTCTATCACTGACTTCGGTATCTTCATCGGCTTGGACGGCGGCATCGACGGCCTGGTTCACCTGTCTGACATCTCCTGGAACGTTGCAGGCGAAGAAGCAGTTCGTGAATACAAAAAAGGCGACGAAATCGCTGCAGTTGTTCTGCAGGTTGACGCAGAACGTGAACGTATCTCCCT  >  NC_000913/963018‑963286

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑