Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,798,834	G→T	H37N (CAC→AAC)	pheS ←	phenylalanine‑‑tRNA ligase subunit alpha

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,798,834	0	G	T	100.0%	142.7 / NA	42	H37N (CAC→AAC)	pheS	phenylalanine‑‑tRNA ligase subunit alpha
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (22/20);  total (22/20)

CGCTTTCCAGTTCCGCTTTACGCGCATTCAGCGCCTGCTGAACCTGCTCTTTCGCTTCGTTGATAACCGCACCAGCTGCCGGACGCTCTTCTGGCGGCAGCTCACGCAGGGTCGTCATCTGAAGGGTTAAGTGCCCTTTTTTACCCAAATATTCGACGCGCACATTATCTAACGCGGCAACATCTGACGCCTGGCTAATGGCCGCCTTCGCACTGGCAACCAGTTCTGCGAGATGTGACATGGTTTTCCTCATTGTGTCAGTGG  >  NC_000913/1798702‑1798965                                                                                                                                     |                                                                                                                                    cgcTTTCCAGTTCCGCTTTACGCGCATTCAGCGCCTGCTGAACCTGCTCTTTCGCTTCGTTGATAACCGCACCAGCTGCCGGACGCTCTTCTGGCGGCAGCTCACGCAGGGTCGTCATCTGAAGGGTTAAGTTcc                                                                                                                                   >  1:297926/1‑135 (MQ=255)  gcTTTCCAGTTCCGCTTTACGCGCATTCAGCGCCTGCTGAACCTGCTCTTTCGCTTCGTTGATAACCGCACCAGCTGCCGGACGCTCTTCTGGCGGCAGCTCACGCAGGGTCGTCATCTGAAGGGTTAAGTTCCCttttt                                                                                                                             >  2:348025/1‑140 (MQ=255)    tttCCAGTTCCGCTTTACGCGCATTCAGCGCCTGCTGAACCTGCTCTTTCGCTTCGTTGATAACCGCACCAGCTGCCGGACGCTCTTCTGGCGGCAGCTCACGCAGGGTCGTCATCTGAAGGGTTAAGTTCCCTTTTTTa                                                                                                                           <  2:455717/140‑1 (MQ=255)      tCCAGTTCCGCTTTACGCGCATTCAGCGCCTGCTGAACCTGCTCTTTCGCTTCGTTGATAACCGCACCAGCTGCCGGACGCTCTTCTGGCGGCAGCTCACGCAGGGTCGTCATCTGAAGGGTTAAGTTCCCtttttaccc                                                                                                                         >  2:408934/1‑136 (MQ=255)      tCCAGTTCCGCTTTACGCGCATTCAGCGCCTGCTGAACCTGCTCTTTCGCTTCGTTGATAACCGCACCAGCTGCCGGACGCTCTTCTGGCGGCAGCTCACGCAGGGTCGTCATCTGAAGGGTTAAGTTCCCTTTTTTAcc                                                                                                                         >  2:43796/1‑140 (MQ=255)           ttCCGCTTTACGCGCATTCAGCGCCTGCTGAACCTGCTCTTTCGCTTCGTTGATAACCGCACCAGCTGCCGGACGCTCTTCTGGCGGCAGCTCACGCAGGGTCGTCATCTGAAGGGTTAAGTTCCCTTTTTTACCCAAat                                                                                                                    >  1:403020/1‑140 (MQ=255)              cGCTTTACGCGCATTCAGCGCCTGCTGAACCTGCTCTTTCGCTTCGTTGATAACCGCACCAGCTGCCGGACGCTCTTCTGGCGGCAGCTCACGCAGGGTCGTCATCTGAAGGGTTAAGTTCCCTTTTTTACCCAAATAtt                                                                                                                 <  2:386383/140‑1 (MQ=255)               gCTTTACGCGCATTCAGCGCCTGCTGAACCTGCTCTTTCGCTTCGTTGATAACCGCACCAGCTGCCGGACGCTCTTCTGGCGGCAGCTCACGCAGGGTCGTCATCTGAAGGGTTAAGTTCCCTTTTTTACCCAAATATTc                                                                                                                <  2:328828/140‑1 (MQ=255)                cTTTACGCGCATTCAGCGCCTGCTGAACCTGCTCTTTCGCTTCGTTGATAACCGCACCAGCTGCCGGACGCTCTTCTGGCGGCAGCTCACGCAGGGTCGTCATCTGAAGGGTTAAGTTCCCTTTTTTACCCAAATATTCg                                                                                                               >  2:232467/1‑140 (MQ=255)                cTTTACGCGCATTCAGCGCCTGCTGAACCTGCTCTTTCGCTTCGTTGATAACCGCACCAGCTGCCGGACGCTCTTCTGGCGGCAGCTCACGCAGGGTCGTCATCTGAAGGGTTAAGTTCCCTTTTTTACCCAAATATTCg                                                                                                               >  1:461021/1‑140 (MQ=255)                   tACGCGCATTCAGCGCCTGCTGAACCTGCTCTTTCGCTTCGTTGATAACCGCACCAGCTGCCGGACGCTCTTCTGGCGGCAGCTCACGCAGGGTCGTCATCTGAAGGGTTAAGTTCCCTTTTTTACCCAAATATTCGAcg                                                                                                            <  1:43796/140‑1 (MQ=255)                    aCGCGCATTCAGCGCCTGCTGAACCTGCTCTTTCGCTTCGTTGATAACCGCACCAGCTGCCGGACGCTCTTCTGGCGGCAGCTCACGCAGGGTCGTCATCTGAAGGGTTAAGTTCCCTTTTTTACCCAAATATTCGAcgc                                                                                                           <  1:171028/140‑1 (MQ=255)                         cATTCAGCGCCTGCTGAACCTGCTCTTTCGCTTCGTTGATAACCGCACCAGCTGCCGGACGCTCTTCTGGCGGCAGCTCACGCAGGGTCGTCATCTGAAGGGTTAAGTTCCCTTTTTTACCCAAATATTCGACGCGcaca                                                                                                      <  1:131964/140‑1 (MQ=255)                                  ccTGCTGAACCTGCTCTTTCGCTTCGTTGATAACCGCACCAGCTGCCGGACGCTCTTCTGGCGGCAGCTCACGCAGGGTCGTCATCTGAAGGGTTAAGTTCCCTTTTTTACCCAAATATTCGACGCGCACATTATCTAAc                                                                                             <  1:28094/140‑1 (MQ=255)                                        gAACCTGCTCTTTCGCTTCGTTGATAACCGCACCAGCTGCCGGACGCTCTTCTGGCGGCAGCTCACGCAGGGTCGTCATCTGAAGGGTTAAGTTCCCTTTTTTACCCAAATATTCGACGCGCACATTATCTAACGCGGCa                                                                                       >  2:360626/1‑140 (MQ=255)                                              gCTCTTTCGCTTCGTTGATAACCGCACCAGCTGCCGGACGCTCTTCTGGCGGCAGCTCACGCAGGGTCGTCATCTGAAGGGTTAAGTTCCCTTTTTTACCCAAATATTCGACGCGCACATTATCTAACGCGGCAACATCt                                                                                 <  1:66102/140‑1 (MQ=255)                                                tctTTCGCTTCGTTGATAACCGCACCAGCTGCCGGACGCTCTTCTGGCGGCAGCTCACGCAGGGTCGTCATCTGAAGGGTTAAGTTCCCTTTTTTACCCAAATATTCGACGCGCACATTATCTAACGCGGCAACATCTGa                                                                               <  2:93061/140‑1 (MQ=255)                                                  tttCGCTTCGTTGATAACCGCACCAGCTGCCGGACGCTCTTCTGGCGGCAGCTCACGCAGGGTCGTCATCTGAAGGGTTAAGTTCCCTTTTTTACCCAAATATTCGACGCGCACATTATCTAACGCGGCAACATCTGACg                                                                             <  1:348025/140‑1 (MQ=255)                                                   ttCGCTTCGTTGATAACCGCACCAGCTGCCGGACGCTCTTCTGGCGGCAGCTCACGCAGGGTCGTCATCTGAAGGGTTAAGTTCCCTTTTTTACCCAAATATTCGACGCGCACATTATCTAACGCGGCAACATCTGACGc                                                                            >  2:125981/1‑140 (MQ=255)                                                   ttCGCTTCGTTGATAACCGCACCAGCTGCCGGACGCTCTTCTGGCGGCAGCTCACGCAGGGTCGTCATCTGAAGGGTTAAGTTCCCTTTTTTACCCAAATATTCGACGCGCACATTATCTAACGCGGCAACATCTGACGc                                                                            >  2:326324/1‑140 (MQ=255)                                                    tCGCTTCGTTGATAACCGCACCAGCTGCCGGACGCTCTTCTGGCGGCAGCTCACGCAGGGTCGTCATCTGAAGGGTTAAGTTCCCTTTTTTACCCAAATATTCGACGCGCACATTATCTAACGCGGCAACATCTGACGcc                                                                           <  1:360626/140‑1 (MQ=255)                                                         tCGTTGATAACCGCACCAGCTGCCGGACGCTCTTCTGGCGGCAGCTCACGCAGGGTCGTCATCTGAAGGGTTAAGTTCCCTTTTTTACCCAAATATTCGACGCGCACATTATCTAACGCGGCAACATCTGACGCCTGGCt                                                                      >  2:395935/1‑140 (MQ=255)                                                            ttGATAACCGCACCAGCTGCCGGACGCTCTTCTGGCGGCAGCTCACGCAGGGTCGTCATCTGAAGGGTTAAGTTCCCTTTTTTACCCAAATATTCGACGCGCACATTATCTAACGCGGCAACATCTGACGCCTGGCTAAt                                                                   >  2:75908/1‑140 (MQ=255)                                                                    cGCACCAGCTGCCGGACGCTCTTCTGGCGGCAGCTCACGCAGGGTCGTCATCTGAAGGGTTAAGTTCCCTTTTTTACCCAAATATTCGACGCGCACATTATCTAACGCGGCAACATCTGACGc                                                                            >  2:100516/1‑123 (MQ=255)                                                                    cGCACCAGCTGCCGGACGCTCTTCTGGCGGCAGCTCACGCAGGGTCGTCATCTGAAGGGTTAAGTTCCCTTTTTTACCCAAATATTCGACGCGCACATTATCTAACGCGGCAACATCTGACGc                                                                            <  1:100516/123‑1 (MQ=255)                                                                    cGCACCAGCTGCCGGACGCTCTTCTGGCGGCAGCTCACGCAGGGTCGTCATCTGAAGGGTTAAGTTCCCTTTTTTACCCAAATATTCGACGCGCACATTATCTAACGCGGCAACATCTGACGCCTGGCTAATGGCCGCCt                                                           >  1:345946/1‑140 (MQ=255)                                                                     gCACCAGCTGCCGGACGCTCTTCTGGCGGCAGCTCACGCAGGGTCGTCATCTGAAGGGTTAAGTTCCCTTTTTTACCCAAATATTCGACGCGCACATTATCTAACGCGGCAACATCTGACGCCTGGCTAATGGCCGCCtt                                                          <  2:461021/140‑1 (MQ=255)                                                                      cACCAGCTGCCGGACGCTCTTCTGGCGGCAGCTCACGCAGGGTCGTCATCTGAAGGGTTAAGTTCCCTTTTTTACCCAAATATTCGACGCGCACATTATCTAACGCGGCAACATCTGACGCCTGGCTAATGGCCGCCTTc                                                         >  2:207408/1‑140 (MQ=255)                                                                      cACCAGCTGCCGGACGCTCTTCTGGCGGCAGCTCACGCAGGGTCGTCATCTGAAGGGTTAAGTTCCCTTTTTTACCCAAATATTCGACGCGCACATTATCTAACGCGGCAACATCTGACGCCTGGCTAATGGCCGCCTTc                                                         >  1:201737/1‑140 (MQ=255)                                                                            cTGCCGGACGCTCTTCTGGCGGCAGCTCACGCAGGGTCGTCATCTGAAGGGTTAAGTTCCCTTTTTTACCCAAATATTCGACGCGCACATTATCTAACGCGGCAACATCTGACGCCTGGCTAATGGCCGCCTTCGCACTg                                                   <  1:122241/140‑1 (MQ=255)                                                                                     gCTCTTCTGGCGGCAGCTCACGCAGGGTCGTCATCTGAAGGGTTAAGTTCCCTTTTTTACCCAAATATTCGACGCGCACATTATCTAACGCGGCAACATCTGACGCCTGGCTAATGGCCGCCTTCGCACTGGCAACCAGt                                          <  1:395935/140‑1 (MQ=255)                                                                                      ctctTCTGGCGGCAGCTCACGCAGGGTCGTCATCTGAAGGGTTAAGTTCCCTTTTTTACCCAAATATTCGACGCGCACATTATCTAACGCGGCAACATCTGACGCCTGGCTAATGGCCGCCTTCGCACTGGCAACCAGtt                                         >  2:231806/1‑140 (MQ=255)                                                                                         ttctGGCGGCAGCTCACGCAGGGTCGTCATCTGAAGGGTTAAGTTCCCTTTTTTACCCAAATATTCGACGCGCACATTATCTAACGCGGCAACATCTGACGCCTGGCTAATGGCCGCCTTCGCACTGGCAACCAGTTCTg                                      <  1:357024/140‑1 (MQ=255)                                                                                                              ggTCGTCATCTGAAGGGTTAAGTTCCCTTTTTTACCCAAATATTCGACGCGCACATTATCTAACGCGGCAACATCTGACGCCTGGCTAATGGCCGCCTTCGCACTGGCAACCAGTTCTGCGAGATGTGACATGGTTTTcc                 <  1:75908/140‑1 (MQ=255)                                                                                                               gtcgtcATCTGAAGGGTTAAGTTCCCTTTTTTACCCAAATATTCGACGCGCACATTATCTAACGCGGCAACATCTGACGCCTGGCTAATGGCCGCCTTCGCACTGGCAACCAGTTCTGCGAGATGTGACATGGTTTTCCt                >  1:76376/1‑140 (MQ=255)                                                                                                               gtcgtcATCTGAAGGGTTAAGTTCCCTTTTTTACCCAAATATTCGACGCGCACATTATCTAACGCGGCAACATCTGACGCCTGGCTAATGGCCGCCTTCGCACTGGCAACCAGTTCTGCGAGATGTGACATGGTTTTCCt                >  1:403164/1‑140 (MQ=255)                                                                                                                  gtcATCTGAAGGGTTAAGTTCCCTTTTTTACCCAAATATTCGACGCGCACATTATCTAACGCGGCAACATCTGACGCCTGGCTAATGGCCGCCTTCGCACTGGCAACCAGTTCTGCGAGATGTGACATGGTTTTCCTCAt             >  1:303058/1‑140 (MQ=255)                                                                                                                       cTGAAGGGTTAAGTTCCCTTTTTTACCCAAATATTCGACGCGCACATTATCTAACGCGGCAACATCTGACGCCTGGCTAATGGCCGCCTTCGCACTGGCAACCAGTTCTGCGAGATGTGACATGGTTTTCCTCATTgtgt        <  2:403020/140‑1 (MQ=255)                                                                                                                        tGAAGGGTTAAGTTCCCTTTTTTACCCAAATATTCGACGCGCACATTATCTAACGCGGCAACATCTGACGCCTGGCTAATGGCCGCCTTCGCACTGGCAACCAGTTCTGCGAGATGTGACATGGTTTTCCTCATTGTGTc       <  2:3248/140‑1 (MQ=255)                                                                                                                        tGAAGGGTTAAGTTCCCTTTTTTACCCAAATATTCGACGCGCACATTATCTAACGCGGCAACATCTGACGCCTGGCTAATGGCCGCCTTCGCACTGGCAACCAGTTCTGCGAGATGTGACATGGTTTTCCTCATTGTGTc       >  1:449635/1‑140 (MQ=255)                                                                                                                             ggTTAAGTTCCCTTTTTTACCCAAATATTCGACGCGCACATTATCTAACGCGGCAACATCTGACGCCTGGCTAATGGCCGCCTTCGCACTGGCAACCAGTTCTGCGAGATGTGACATGGTTTTCCTCATTGTGTCAgtgg  >  1:94365/1‑140 (MQ=255)                                                                                                                             ggTTAAGTTCCCTTTTTTACCCAAATATTCGACGCGCACATTATCTAACGCGGCAACATCTGACGCCTGGCTAATGGCCGCCTTCGCACTGGCAACCAGTTCTGCGAGATGTGACATGGTTTTCCTCATTGTGTCAgtgg  <  1:231806/140‑1 (MQ=255)                                                                                                                                     |                                                                                                                                    CGCTTTCCAGTTCCGCTTTACGCGCATTCAGCGCCTGCTGAACCTGCTCTTTCGCTTCGTTGATAACCGCACCAGCTGCCGGACGCTCTTCTGGCGGCAGCTCACGCAGGGTCGTCATCTGAAGGGTTAAGTGCCCTTTTTTACCCAAATATTCGACGCGCACATTATCTAACGCGGCAACATCTGACGCCTGGCTAATGGCCGCCTTCGCACTGGCAACCAGTTCTGCGAGATGTGACATGGTTTTCCTCATTGTGTCAGTGG  >  NC_000913/1798702‑1798965

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑