Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,715,011	C→A	intergenic (+29/+15)	gstA → / ← pdxY	glutathione S‑transferase GstA/pyridoxal kinase 2

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,715,011	0	C	A	100.0%	48.8 / NA	15	intergenic (+29/+15)	gstA/pdxY	glutathione S‑transferase GstA/pyridoxal kinase 2
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (6/9);  total (6/9)

GGTGAAACTGAATCTGGAAGGGTTAGAGCACATTGCAGCATTTATGCAACGTATGGCTGAACGTCCGGAAGTACAAGACGCGCTGTCAGCGGAAGGCTTAAAGTAATAGCCATCGGCCCACAGGTCGTTGTGGGCCGAAATGAGATATTTCAGAGCTTTGTTGCGCTGAAGTAATGTTCTGGTTTGGCAATACGATCCTGAGCAGCCACCACTTGCAGCTCATATTCCTGC  >  NC_000913/1714877‑1715107                                                                                                                                       |                                                                                                 ggTGAAACTGAATCTGGAAGGGTTAGAGCACATTGCAGCATTTATGCAACGTATGGCTGAACGTCCGGAAGTACAAGACGCGCTGTCAGCGGAAGGCTTAAAGTAATAGCCATCGGCCCACAGGTCGTTGTGGGACGaaa                                                                                             <  2:405364/140‑1 (MQ=255)  gTGAAACTGAATCTGGAAGGGTTAGAGCACATTGCAGCATTTATGCAACGTATGGCTGAACGTCCGGAAGTACAAGACGCGCTGTCAGCGGAAGGCTTAAAGTAATAGCCATCGGCCCACAGGTCGTTGTGGGACGAAAt                                                                                            >  2:119995/1‑140 (MQ=255)                                            aTGCAACGTATGGCTGAACGTCCGGAAGTACAAGACGCGCTGTCAGCGGAAGGCTTAAAGTAATAGCCATCGGCCCACAGGTCGTTGTGGGACGAAATGAGATATTTCAGAGCTTTGTTGCGCTGAAGTAATGTTCTGGt                                                  >  1:133358/1‑140 (MQ=255)                                            aTGCAACGTATGGCTGAACGTCCGGAAGTACAAGACGCGCTGTCAGCGGAAGGCTTAAAGTAATAGCCATCGGCCCACAGGTCGTTGTGGGACGAAATGAGATATTTCAGAGCTTTGTTGCGCTGAAGTAATGTTCTGGt                                                  >  1:333481/1‑140 (MQ=255)                                            aTGCAACGTATGGCTGAACGTCCGGAAGTACAAGACGCGCTGTCAGCGGAAGGCTTAAAGTAATAGCCATCGGCCCACAGGTCGTTGTGGGACGAAATGAGATATTTCAGAGCTTTGTTGCGCTGAAGTAATGTTCTGGt                                                  <  1:83293/140‑1 (MQ=255)                                                aaCGTATGGCTGAACGTCCGGAAGTACAAGACGCGCTGTCAGCGGAAGGCTTAAAGTAATAGCCATCGGCCCACAGGTCGTTGTGGGACGAAATGAGATATTTCAGAGCTTTGTTGCGCTGAAGTAATGTTCTGGTTTgg                                              <  2:309498/140‑1 (MQ=255)                                                     aTGGCTGAACGTCCGGAAGTACAAGACGCGCTGTCAGCGGAAGGCTTAAAGTAATAGCCATCGGCCCACAGGTCGTTGTGGGACGAAATGAGATATTTCAGAGCTTTGTTGCGCTGAAGTAATGTTCTGGTTTGGCAATa                                         <  2:333481/140‑1 (MQ=255)                                                       ggCTGAACGTCCGGAAGTACAAGACGCGCTGTCAGCGGAAGGCTTAAAGTAATAGCCATCGGCCCACAGGTCGTTGTGGGACGAAATGAGATATTTCAGAGCTTTGTTGCGCTGAAGTAATGTTCTGGTTTGGCAATACg                                       <  2:187374/140‑1 (MQ=255)                                                                  cGGAAGTACAAGACGCGCTGTCAGCGGAAGGCTTAAAGTAATAGCCATCGGCCCACAGGTCGTTGTGGGACGAAATGAGATATTTCAGAGCTTTGTTGCGCTGAAGTAATGTTCTGGTTTGGCAATACGATCCTGagcag                            <  1:119995/140‑1 (MQ=255)                                                                      aGTACAAGACGCGCTGTCAGCGGAAGGCTTAAAGTAATAGCCATCGGCCCACAGGTCGTTGTGGGACGAAATGAGATATTTCAGAGCTTTGTTGCGCTGAAGTAATGTTCTGGTTTGGCAATACGATCCTGAGCAGccac                        <  1:343118/140‑1 (MQ=255)                                                                               cgcgCTGTCAGCGGAAGGCTTAAAGTAATAGCCATCGGCCCACAGGTCGTTGTGGGACGAAATGAGATATTTCAGAGCTTTGTTGCGCTGAAGTAATGTTCTGGTTTGGCAATACGATCCTGAGCAGCCACCACTTg                  <  1:95695/137‑1 (MQ=255)                                                                               cgcgCTGTCAGCGGAAGGCTTAAAGTAATAGCCATCGGCCCACAGGTCGTTGTGGGACGAAATGAGATATTTCAGAGCTTTGTTGCGCTGAAGTAATGTTCTGGTTTGGCAATACGATCCTGAGCAGCCACCACTTg                  >  2:95695/1‑137 (MQ=255)                                                                               cgcgCTGTCAGCGGAAGGCTTAAAGTAATAGCCATCGGCCCACAGGTCGTTGTGGGACGAAATGAGATATTTCAGAGCTTTGTTGCGCTGAAGTAATGTTCTGGTTTGGCAATACGATCCTGAGCAGCCACCACTTGCAg               >  2:271908/1‑140 (MQ=255)                                                                                       cAGCGGAAGGCTTAAAGTAATAGCCATCGGCCCACAGGTCGTTGTGGGACGAAATGAGATATTTCAGAGCTTTGTTGCGCTGAAGTAATGTTCTGGTTTGGCAATACGATCCTGAGCAGCCACCACTTGCAGCTCATAtt       >  1:203073/1‑140 (MQ=255)                                                                                            gAAGGCTTAAAGTAATAGCCATCGGCCCACAGGTCGTTGTGGGACGAAATGAGATATTTCAGAGCTTTGTTGCGCTGAAGTAATGTTCTGGTTTGGCAATACGATCCTGAGCAGCCACCACTTGCAGCTCATATTCCTGc  <  1:83575/140‑1 (MQ=255)                                                                                                                                       |                                                                                                 GGTGAAACTGAATCTGGAAGGGTTAGAGCACATTGCAGCATTTATGCAACGTATGGCTGAACGTCCGGAAGTACAAGACGCGCTGTCAGCGGAAGGCTTAAAGTAATAGCCATCGGCCCACAGGTCGTTGTGGGCCGAAATGAGATATTTCAGAGCTTTGTTGCGCTGAAGTAATGTTCTGGTTTGGCAATACGATCCTGAGCAGCCACCACTTGCAGCTCATATTCCTGC  >  NC_000913/1714877‑1715107

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑