Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,078,265	C→A	intergenic (+131/‑310)	yeeW → / → yoeF	CP4‑44 prophage; putative uncharacterized protein YeeW/CP4‑44 prophage; putative uncharacterized protein YoeF

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,078,265	0	C	A	100.0%	153.4 / NA	44	intergenic (+131/‑310)	yeeW/yoeF	CP4‑44 prophage; putative uncharacterized protein YeeW/CP4‑44 prophage; putative uncharacterized protein YoeF
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (20/24);  total (20/24)

GTAAGACAATGCAGTGATACAGGGTATAGTGCCATTAACCACAGAAACAGCACTGTTGTCCTGTATTAAGCAGGCAAGAGAAGATGTCCTTACCCTGCGCCATCTGCAGCTTCTGCACCAGAACCGGTGAATCTCACTCAACAGGCTTCATTTCTCCTGACGCCAGCCTGAATACAGCTGGCGTTTTCATTTATAAACAGAAAGGAAAACCGATAATAATGGAATGGACGCCCCGACCATGAAGCAACGGTAGGATATTAC  >  NC_000913/2078131‑2078391                                                                                                                                       |                                                                                                                               gTAAGACAATGCAGTGATACAGGGTATAGTGCCATTAACCACAGAAACAGCACTGTTGTCCTGTATTAAGCAGGCAAGAGAAGATGTCCTTACCCTGCGCCATCTGCAGCTTCTGCACCAGAACCGGTGAATctaactca                                                                                                                           >  1:68416/1‑140 (MQ=255) gTAAGACAATGCAGTGATACAGGGTATAGTGCCATTAACCACAGAAACAGCACTGTTGTCCTGTATTAAGCAGGCAAGAGAAGATGTCCTTACCCTGCGCCATCTGCAGCTTCTGCACCAGAACCGGTGAATctaactca                                                                                                                           >  2:391080/1‑140 (MQ=255) gTAAGACAATGCAGTGATACAGGGTATAGTGCCATTAACCACAGAAACAGCACTGTTGTCCTGTATTAAGCAGGCAAGAGAAGATGTCCTTACCCTGCGCCATCTGCAGCTTCTGCACCAGAACCGGTGAATctaactca                                                                                                                           >  1:184967/1‑140 (MQ=255)       cAATGCAGTGATACAGGGTATAGTGCCATTAACCACAGAAACAGCACTGTTGTCCTGTATTAAGCAGGCAAGAGAAGATGTCCTTACCCTGCGCCATCTGCAGCTTCTGCACCAGAACCGGTGAATCTAACTCAACAGGc                                                                                                                     <  1:328274/140‑1 (MQ=255)          tGCAGTGATACAGGGTATAGTGCCATTAACCACAGAAACAGCACTGTTGTCCTGTATTAAGCAGGCAAGAGAAGATGTCCTTACCCTGCGCCATCTGCAGCTTCTGCACCAGAACCGGTGAATCTAACTCAGCAGGCTTc                                                                                                                  >  1:145071/1‑140 (MQ=255)          tGCAGTGATACAGGGTATAGTGCCATTAACCACAGAAACAGCACTGTTGTCCTGTATTAAGCAGGCAAGAGAAGATGTCCTTACCCTGCGCCATCTGCAGCTTCTGCACCAGAACCGGTGAATCTAACTCAACAGGCTTc                                                                                                                  >  2:158826/1‑140 (MQ=255)              gTGATACAGGGTATAGTGCCATTAACCACAGAAACAGCACTGTTGTCCTGTATTAAGCAGGCAAGAGAAGATGTCCTTACCCTGCGCCATCTGCAGCTTCTGCACCAGAACCGGTGAATCTAACTCAACAGGCTTCAttt                                                                                                              <  1:158826/140‑1 (MQ=255)                  tACAGGGTATAGTGCCATTAACCACAGAAACAGCACTGTTGTCCTGTATTAAGCAGGCAAGAGAAGATGTCCTTACCCTGCGCCATCTGCAGCTTCTGCACCAGAACCGGTGAATCTAACTCAACAGGCTTCATTTCTcc                                                                                                          <  1:267656/140‑1 (MQ=255)                       ggTATAGTGCCATTAACCACAGAAACAGCACTGTTGTCCTGTATTAAGCAGGCAAGAGAAGATGTCCTTACCCTGCGCCATCTGCAGCTTCTGCACCAGAACCGGTGAATCTAACTCAACAGGCTTCATTTCTCCTGACg                                                                                                     <  2:184967/140‑1 (MQ=255)                         tataGTGCCATTAACCACAGAAACAGCACTGTTGTCCTGTATTAAGCAGGCAAGAGAAGATGTCCTTACCCTGCGCCATCTGCAGCTTCTGCACCAGAACCGGTGAATCTAACTCAACAGGCTTCATTTCTCCTGACGcc                                                                                                   <  2:257243/140‑1 (MQ=255)                           taGTGCCATTAACCACAGAAACAGCACTGTTGTCCTGTATTAAGCAGGCAAGAGAAGATGTCCTTACCCTGCGCCATCTGCAGCTTCTGCACCAGAACCGGTGAATCTAACTCAACAGGCTTCATTTCTCCTGACGCCAg                                                                                                 <  1:177775/140‑1 (MQ=255)                            aGTGCCATTAACCACAGAAACAGCACTGTTGTCCTGTATTAAGCAGGCAAGAGAAGATGTCCTTACCCTGCGCCATCTGCAGCTTCTGCACCAGAACCGGTGAATCTAACTCAACAGGCTTCATTTCTCCTGACGCCAGc                                                                                                <  1:321198/140‑1 (MQ=255)                              tGCCATTAACCACAGAAACAGCACTGTTGTCCTGTATTAAGCAGGCAAGAGAAGATGTCCTTACCCTGCGCCATCTGCAGCTTCTGCACCAGAACCGGTGAATCTAACTCAACAGGCTTCAtt                                                                                                               <  1:282340/123‑1 (MQ=255)                              tGCCATTAACCACAGAAACAGCACTGTTGTCCTGTATTAAGCAGGCAAGAGAAGATGTCCTTACCCTGCGCCATCTGCAGCTTCTGCACCAGAACCGGTGAATCTAACTCAACAGGCTTCAtt                                                                                                               >  2:282340/1‑123 (MQ=255)                               gCCATTAACCACAGAAACAGCACTGTTGTCCTGTATTAAGCAGGCAAGAGAAGATGTCCTTACCCTGCGCCATCTGCAGCTTCTGCACCAGAACCGGTGAATCTAACTCAACAGGCTTCATTTCTCCTGACGCCAGCCTg                                                                                             >  2:95095/1‑140 (MQ=255)                                 cATTAACCACAGAAACAGCACTGTTGTCCTGTATTAAGCAGGCAAGAGAAGATGTCCTTACCCTGCGCCATCTGCAGCTTCTGCACCAGAACCGGTGAATCTAACTCAGCAGGCTTCATTTCTCCTGACGCCAGCCTGaa                                                                                           <  2:145071/140‑1 (MQ=255)                                   ttAACCACAGAAACAGCACTGTTGTCCTGTATTAAGCAGGCAAGAGAAGATGTCCTTACCCTGCGCCATCTGCAGCTTCTGCACCAGAACCGGTGAATCTAACTCAACAGGCTTCATTTCTCCTGACGcc                                                                                                   <  1:143511/130‑1 (MQ=255)                                   ttAACCACAGAAACAGCACTGTTGTCCTGTATTAAGCAGGCAAGAGAAGATGTCCTTACCCTGCGCCATCTGCAGCTTCTGCACCAGAACCGGTGAATCTAACTCAACAGGCTTCATTTCTCCTGACGcc                                                                                                   >  2:143511/1‑130 (MQ=255)                                      aCCACAGAAACAGCACTGTTGTCCTGTATTAAGCAGGCAAGAGAAGATGTCCTTACCCTGCGCCATCTGCAGCTTCTGCACCAGAACCGGTGAATCTAACTCAACAGGCTTCATTTCTCCTGACGCCAGCCTGAATACAg                                                                                      <  1:391080/140‑1 (MQ=255)                                                 aGCACTGTTGTCCTGTATTAAGCAGGCAAGAGAAGATGTCCTTACCCTGCGCCATCTGCAGCTTCTGCACCAGAACCGGTGAATCTAACTCAACAGGCTTCATTTCTCCTGACGCCAGCCTGAATACAGCTGGCGTTTTc                                                                           >  1:363468/1‑140 (MQ=255)                                                   cACTGTTGTCCTGTATTAAGCAGGCAAGAGAAGATGTCCTTACCCTGCGCCATCTGCAGCTTCTGCACCAGAACCGGTGAATCTAACTCAACAGGCTTCATTTCTCCTGACGCCAGCCTGAATACAGCTGGCGTTTTCAt                                                                         <  1:95095/140‑1 (MQ=255)                                                             cTGTATTAAGCAGGCAAGAGAAGATGTCCTTACCCTGCGCCATCTGCAGCTTCTGCACCAGAACCGGTGAATCTAACTCAACAGGCTTCATTTCTCCTGACGCCAGCCTGAATACAGCTGGCGTTTTCATTTATAAACAg                                                               >  2:131427/1‑140 (MQ=255)                                                                tATTAAGCAGGCAAGAGAAGATGTCCTTACCCTGCGCCATCTGCAGCTTCTGCACCAGAACCGGTGAATCTAACTCAACAGGCTTCATTTCTCCTGACGCCAGCCTGAATACAGCTGGCGTTTTCATTTATAAACAGaaa                                                            >  2:75467/1‑140 (MQ=255)                                                                tATTAAGCAGGCAAGAGAAGATGTCCTTACCCTGCGCCATCTGCAGCTTCTGCACCAGAACCGGTGAATCTAACTCAACAGGCTTCATTTCTCCTGACGCCAGCCTGAATACAGCTGGCGTTTTCATTTATAAACAGaaa                                                            >  2:223614/1‑140 (MQ=255)                                                                   tAAGCAGGCAAGAGAAGATGTCCTTACCCTGCGCCATCTGCAGCTTCTGCACCAGAACCGGTGAATCTAACTCAACAGGCTTCATTTCTCCTGACGCCAGCCTGAATACAGCTGGCGTTTTCATTTATAAACAGAAAGGa                                                         <  1:358423/140‑1 (MQ=255)                                                                     aGCAGGCAAGAGAAGATGTCCTTACCCTGCGCCATCTGCAGCTTCTGCACCAGAACCGGTGAATCTAACTCAACAGGCTTCATTTCTCCTGACGCCAGCCTGAATACAGCTGGCGTTTTCATTTATAAACAGAAAGGaaa                                                       >  2:379183/1‑140 (MQ=255)                                                                       cAGGCAAGAGAAGATGTCCTTACCCTGCGCCATCTGCAGCTTCTGCACCAGAACCGGTGAATCTAACTCAACAGGCTTCATTTCTCCTGACGCCAGCCTGAATACAGCTGGCGTTTTCATTTATAAACAGAAAGGAAAAc                                                     <  1:131427/140‑1 (MQ=255)                                                                          gCAAGAGAAGATGTCCTTACCCTGCGCCATCTGCAGCTTCTGCACCAGAACCGGTGAATCTAACTCAACAGGCTTCATTTCTCCTGACGCCAGCCTGAATACAGCTGGCGTTTTCATTTATAAACAGAAAGGAAAACCGa                                                  >  1:401095/1‑140 (MQ=255)                                                                                       tCCTTACCCTGCGCCATCTGCAGCTTCTGCACCAGAACCGGTGAATCTAACTCAACAGGCTTCATTTCTCCTGACGCCAGCCTGAATACAGCTGGCGTTTTCATTTATAAACAGAAAGGAAAACCGATAATaatggaatg                                     <  2:401095/140‑1 (MQ=255)                                                                                           tACCCTGCGCCATCTGCAGCTTCTGCACCAGAACCGGTGAATCTAACTCAACAGGCTTCATTTCTCCTGACGCCAGCCTGAATACAGCTGGCGTTTTCATTTATAAACAGAAAGGAAAACCGATAATAATGGAATGGACg                                 >  1:60040/1‑140 (MQ=255)                                                                                               cTGCGCCATCTGCAGCTTCTGCACCAGAACCGGTGAATCTAACTCAACAGGCTTCATTTCTCCTGACGCCAGCCTGAATACAGCTGGCGTTTTCATTTATAAACAGAAAGGAAAACCGATAGTAATGGAATGGACGcccc                             <  1:75467/140‑1 (MQ=255)                                                                                                  cgcCATCTGCAGCTTCTGCACCAGAACCGGTGAATCTAACTCAACAGGCTTCATTTCTCCTGACGCCAGCCTGAATACAGCTGGCGTTTTCATTTATAAACAGAAAGGAAAACCGATAATAATGGAATGGACGCCCCGAc                          >  2:175250/1‑140 (MQ=255)                                                                                                  cgcCATCTGCAGCTTCTGCACCAGAACCGGTGAATCTAACTCAACAGGCTTCATTTCTCCTGACGCCAGCCTGAATACAGCTGGCGTTTTCATTTATAAACAGAAAGGAAAACCGATAATAATGGAATGGACGCCCCGAc                          >  2:202014/1‑140 (MQ=255)                                                                                                  cgcCATCTGCAGCTTCTGCACCAGAACCGGCGAATCTAACTCAACAGGCTTCATTTCTCCTGACGCCAGCCTGAATACAGCTGGCGTTTTCATTTATAAACAGAAAGGAAAACCGATAATAATGGAATGGACGCCCCGAc                          >  2:200886/1‑140 (MQ=255)                                                                                                       tCTGCAGCTTCTGCACCAGAACCGGTGAATCTAACTCAACAGGCTTCATTTCTCCTGACGCCAGCCTGAATACAGCTGGCGTTTTCATTTATAAACAGAAAGGAAAACCGATAATAATGGAATGGACGCCCCGACCATGa                     >  2:408464/1‑140 (MQ=255)                                                                                                          gCAGCTTCTGCACCAGAACCGGTGAATCTAACTCAACAGGCTTCATTTCTCCTGACGCCAGCCTGAATACAGCTGGCGTTTTCATTTATAAACAGAAAGGAAAACCGATAATAATGGAATGGACGCCCCGACCATGAAGc                  <  1:202014/140‑1 (MQ=255)                                                                                                          gCAGCTTCTGCACCAGAACCGGTGAATCTAACTCAACAGGCTTCATTTCTCCTGACGCCAGCCTGAATACAGCTGGCGTTTTCATTTATAAACAGAAAGGAAAACCGATAATAATGGAATGGACGCCCCGACCATGAAGc                  <  1:408464/140‑1 (MQ=255)                                                                                                            aGCTTCTGCACCAGAACCGGTGAATCTAACTCAACAGGCTTCATTTCTCCTGACGCCAGCCTGAATACAGCTGGCGTTTTCATTTATAAACAGAAAGGAAAACCGATAATAATGGAGTGGACGCCCCGACCATGAAGCaa                <  1:223614/140‑1 (MQ=255)                                                                                                            aGCTTCTGCACCAGAACCGGTGAATCTAACTCAACAGGCTTCATTTCTCCTGACGCCAGCCTGAATACAGCTGGCGTTTTCATTTATAAACAGAAAGGAAAACCGATAATAATGGAATGGACGCCCCGACCATGAAGCaa                <  1:333115/140‑1 (MQ=255)                                                                                                               ttCTGCACCAGAACCGGTGAATCTAACTCAACAGGCTTCATTTCTCCTGACGCCAGCCTGAATACAGCTGGCGTTTTCATTTATAAACAGAAAGGAAAACCGATAATAATGGAATGGACGCCCCGACCATGAAGCAACgg             <  1:175250/140‑1 (MQ=255)                                                                                                                  tGCACCAGAACCGGTGAATCTAACTCAACAGGCTTCATTTCTCCTGACGCCAGCCTGAATACAGCTGGCGTTTTCATTTATAAACAGAAAGGAAAACCGATAATAATGGAATGGACGCCCCGACCATGAAGc                  >  1:319295/1‑132 (MQ=255)                                                                                                                  tGCACCAGAACCGGTGAATCTAACTCAACAGGCTTCATTTCTCCTGACGCCAGCCTGAATACAGCTGGCGTTTTCATTTATAAACAGAAAGGAAAACCGATAATAATGGAATGGACGCCCCGACCATGAAGc                  <  2:319295/132‑1 (MQ=255)                                                                                                                   gCACCAGAACCGGTGAATCTAACTCAACAGGCTTCATTTCTCCTGACGCCAGCCTGAATACAGCTGGCGTTTTCATTTATAAACAGAAAGGAAAACCGATAATAATGGAATGGACGCCCCGACCATGAAGCAACGGTAgg         >  2:137263/1‑140 (MQ=255)                                                                                                                   gCACCAGAACCGGTGAATCTAACTCAACAGGCTTCATTTCTCCTGACGCCAGCCTGAATACAGCTGGCGTTTTCATTTATAAACAGAAAGGAAAACCGATAATAATGGAATGGACGCCCCGACCATGAAGCAACGGTAgg         <  2:60040/140‑1 (MQ=255)                                                                                                                   gCACCAGAACCGGTGAATCTAACTCAACAGGCTTCATTTCTCCTGACGCCAGCCTGAATACAGCTGGCGTTTTCATTTATAAACAGAAAGGAAAACCGATAATAATGGAATGGACGCCCCGACCATGAAGCAACGGTAgg         <  1:379183/140‑1 (MQ=255)                                                                                                                         gAACCGGTGAATCTAACTCAACAGGCTTCATTTCTCCTGACGCCAGCCTGAATACAGCTGGCGTTTTCATTTATAAACAGAAAGGAAAACCGATAATAATGGAATGGACGCCCCGACCATGAAGCAACGGTAGGATATTa   >  1:363711/1‑140 (MQ=255)                                                                                                                          aaCCGGTGAATCTAACTCAACAGGCTTCATTTCTCCTGACGCCAGCCTGAATACAGCTGGCGTTTTCATTTATAAACAGAAAGGAAAACCGATAATAATGGAATGGACGCCCCGACCATGAAGCAACGGTAGGATATTAc  >  1:289372/1‑140 (MQ=255)                                                                                                                                       |                                                                                                                               GTAAGACAATGCAGTGATACAGGGTATAGTGCCATTAACCACAGAAACAGCACTGTTGTCCTGTATTAAGCAGGCAAGAGAAGATGTCCTTACCCTGCGCCATCTGCAGCTTCTGCACCAGAACCGGTGAATCTCACTCAACAGGCTTCATTTCTCCTGACGCCAGCCTGAATACAGCTGGCGTTTTCATTTATAAACAGAAAGGAAAACCGATAATAATGGAATGGACGCCCCGACCATGAAGCAACGGTAGGATATTAC  >  NC_000913/2078131‑2078391

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑