Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,610,205	G→T	A14A (GCG→GCT)	hyfH →	hydrogenase 4 component H

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,610,205	0	G	T	100.0%	62.2 / NA	19	A14A (GCG→GCT)	hyfH	hydrogenase 4 component H
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (11/8);  total (11/8)

GCGCAAGCGCCAGTCAAAAACCGTGCCGTATAAAGAGATCGAACGCTACGGCATTGATCGTAACCGTTCGCCGCTGAAGTAAGGACAGAAGATGCTGAAGTTACTGAAAACTATTATGCGCGCCGGAACCGCGACGGTGAAATATCCCTTCGCGCCACTGGAGGTCAGCCCTGGCTTTCGCGGAAAACCGGACCTGATGCCCAGCCAATGTATTGCCTGCGGTGCCTGCGCCTGTGCTTGTCCGGCAAATGCGCTGACTAT  >  NC_000913/2610073‑2610333                                                                                                                                     |                                                                                                                                 gcgcAAGCGCCAGTCAAAAACCGTGCCGTATAAAGAGATCGAACGCTACGGCATTGATCGTAACCGTTCGCCGCTGAAGTAAGGACAGAAGATGCTGAAGTTACTGAAAACTATTATGCGCGCCGGAACCGCTACGGTGa                                                                                                                           >  1:205158/1‑140 (MQ=255)  cgcAAGCGCCAGTCAAAAACCGTGCCGTATAAAGAGATCGAACGCTACGGCATTGATCGTAACCGTTCGCCGCTGAAGTAAGGACAGAAGATGCTGAAGTTACTGAAAACTATTATGCGCGCCGGAACCGCTACGGTGaa                                                                                                                          >  2:38802/1‑140 (MQ=255)   gcAAGCGCCAGTCAAAAACCGTGCCGTATAAAGAGATCGAACGCTACGGCATTGATCGTAACCGTTCGCCGCTGAAGTAAGGACAGAAGATGCTGAAGTTACTGAAAACTATTATGCGCGCCGGAACCGCTACGGTGaaa                                                                                                                         >  1:82523/1‑140 (MQ=255)      aGCGCCAGTCAAAAACCGTGCCGTATAAAGAGATCGAACGCTACGGCATTGATCGTAACCGTTCGCCGCTGAAGTAAGGACAGAAGATGCTGAAGTTACTGAAAACTATTATGCGCGCCGGAACCGCTACGGTGAAatat                                                                                                                      <  1:38802/140‑1 (MQ=255)                                       tCGAACGCTACGGCATTGATCGTAACCGTTCGCCGCTGAAGTAAGGACAGAAGATGCTGAAGTTACTGAAAACTATTATGCGCGCCGGAACCGCTACGGTGAAATATCCCTTCGCGCCACTGGAGGt                                                                                                  >  1:19049/1‑127 (MQ=255)                                       tCGAACGCTACGGCATTGATCGTAACCGTTCGCCGCTGAAGTAAGGACAGAAGATGCTGAAGTTACTGAAAACTATTATGCGCGCCGGAACCGCTACGGTGAAATATCCCTTCGCGCCACTGGAGGt                                                                                                  <  2:19049/127‑1 (MQ=255)                                           aCGCTACGGCATTGATCGTAACCGTTCGCCGCTGAAGTAAGGACAGAAGATGCTGAAGTTACTGAAAACTATTATGCGCGCCGGAACCGCTACGGTGAAATATCCCTTCGCGCCACTGGAGGt                                                                                                  <  2:90170/123‑1 (MQ=255)                                           aCGCTACGGCATTGATCGTAACCGTTCGCCGCTGAAGTAAGGACAGAAGATGCTGAAGTTACTGAAAACTATTATGCGCGCCGGAACCGCTACGGTGAAATATCCCTTCGCGCCACTGGAGGt                                                                                                  >  1:90170/1‑123 (MQ=255)                                            cGCTACGGCATTGATCGTAACCGTTCGCCGCTGAAGTAAGGACAGAAGATGCTGAAGTTACTGAAAACTATTATGCGCGCCGGAACCGCTACGGTGAAATATCCCTTCGCGCCACTGGAGGTCAGCCCTGGCTTTCGCgg                                                                                <  2:395223/140‑1 (MQ=255)                                                    cATTGATCGTAACCGTTCGCCGCTGAAGTAAGGACAGAAGATGCTGAAGTTACTGAAAACTATTATGCGCGCCGGAACCGCTACGGTGAAATATCCCTTCGCGCCACTGGAGGTCAGCCCTGGCTTTCGCGGAAAACCgg                                                                        <  2:205158/140‑1 (MQ=255)                                                         aTCGTAACCGTTCGCCGCTGAAGTAAGGACAGAAGATGCTGAAGTTACTGAAAACTATTATGCGCGCCGGAACCGCTACGGTGAAATATCCCTTCGCGCCACTGGAGGTCAGCCCTGGCTTTCGCGGAAAACCGGACCTg                                                                   >  1:7231/1‑140 (MQ=255)                                                         aTCGTAACCGTTCGCCGCTGAAGTAAGGACAGAAGATGCTGAAGTTACTGAAAACTATTATGCGCGCCGGAACCGCTACGGTGAAATATCCCTTCGCGCCACTGGAGGTCAGCCCTGGCTTTCGCGGAAAACCGGACCTg                                                                   >  2:371006/1‑140 (MQ=255)                                                                   ttCGCCGCTGAAGTAAGGACAGAAGATGCTGAAGTTACTGAAAACTATTATGCGCGCCGGAACCGCTACGGTGAAATATCCCTTCGCGCCACTGGAGGTCAGCCCTGGCTTTCGCGGAAAACCGGACCTGATGCCCAGcc                                                         <  1:21260/140‑1 (MQ=255)                                                                     cgccgcTGAAGTAAGGACAGAAGATGCTGAAGTTACTGAAAACTATTATGCGCGCCGGAACCGCTACGGTGAAATATCCCTTCGCGCCACTGGAGGTCAGCCCTGGCTTTCGCGGAAAACCGGACCTGATGCCCAGCCaa                                                       <  2:377331/140‑1 (MQ=255)                                                                                  aGGACAGAAGATGCTGAAGTTACTGAAAACTATTATGCGCGCCGGAACCGCTACGGTGAAATATCCCTTCGCGCCACTGGAGGTCAGCCCTGGCTTTCGCGGAAAACCGGACCTGATGCCCAGCCAATGTATTGCCTGCg                                          <  2:82523/140‑1 (MQ=255)                                                                                              gCTGAAGTTACTGAAAACTATTATGCGCGCCGGAACCGCTACGGTGAAATATCCCTTCGCGCCACTGGAGGTCAGCCCTGGCTTTCGCGGAAAACCGGACCTGATGCCCAGCCAATGTATTGCCTGCGGTGCCTGCGCCt                              >  1:123149/1‑140 (MQ=255)                                                                                                                       cgcgcCGGAACCGCTACGGTGAAATATCCCTTCGCGCCACTGGAGGTCAGCCCTGGCTTTCGCGGAAAACCGGACCTGATGCCCAGCCAATGTATTGCCTGCGGTGCCTGCGCCTGTGCTTGTCCGGCAAATGCGCTGAc     >  1:249594/1‑140 (MQ=255)                                                                                                                          gcCGGAACCGCTACGGTGAAATATCCCTTCGCGCCACTGGAGGTCAGCCCTGGCTTTCGCGGAAAACCGGACCTGATGCCCAGCCAATGTATTGCCTGCGGTGCCTGCGCCTGTGCTTGTCCGGCAAATGCGCTGACTAt  >  1:341262/1‑140 (MQ=255)                                                                                                                          gcCGGAACCGCTACGGTGAAACATCCCTTCGCGCCACTGGAGGTCAGCCCTGGCTTTCGCGGAAAACCGGACCTGATGCCCAGCCAATGTATTGCCTGCGGTGCCTGCGCCTGTGCTTGTCCGGCAAATGCGCTGACTAt  >  2:338159/1‑140 (MQ=255)                                                                                                                                     |                                                                                                                                 GCGCAAGCGCCAGTCAAAAACCGTGCCGTATAAAGAGATCGAACGCTACGGCATTGATCGTAACCGTTCGCCGCTGAAGTAAGGACAGAAGATGCTGAAGTTACTGAAAACTATTATGCGCGCCGGAACCGCGACGGTGAAATATCCCTTCGCGCCACTGGAGGTCAGCCCTGGCTTTCGCGGAAAACCGGACCTGATGCCCAGCCAATGTATTGCCTGCGGTGCCTGCGCCTGTGCTTGTCCGGCAAATGCGCTGACTAT  >  NC_000913/2610073‑2610333

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑