Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,270,858	G→T	P305T (CCG→ACG)	garK ←	glycerate 2‑kinase 1

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,270,858	0	G	T	100.0%	142.2 / NA	41	P305T (CCG→ACG)	garK	glycerate 2‑kinase 1
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (20/21);  total (20/21)

TACCTATGCTGGTCAATACGCTGAAGACCGCATCAATGCCATGCTGATGTACAACGCCAACATCATCGGTCAGGCTACCCGCAATGCCAATCACCGGTTTATGGTACTTCTTCGCCACGTTTGCGACACCAATCGGTACCTTCCCGTGAATACTCTGGCTGTCAATACGCCCTTCACCGGTGATCACCAGCGTACAATCGTGAATATGTTCCTCCAGATTCAGCGCCGTAGTGACGATTTCAATACCACTTTTCAGTTCCGCACCAAG  >  NC_000913/3270723‑3270990                                                                                                                                        |                                                                                                                                     tACCTATGCTGGTCAATACGCTGAAGACCGCATCAATGCCATGCTGATGTACAACGCCAACATCATCGGTCAGGCTACCCGCAATGCCAATCACCGGTTTATGGTACTTCTTCGCCACGTTTGCGACCCCAATCGTTAcc                                                                                                                                  <  2:365275/140‑1 (MQ=255)        gCTGGTCAATACGCTGAAGACCGCATCAATGCCATGCTGATGTACAACGCCAACATCATCGGTCAGGCTACCCGCAATGCCAATCACCGGTTTATGGTACTTCTTCGCCACGTTTGCGACACCAATCGTTACCTTCCCGt                                                                                                                           >  2:11822/1‑140 (MQ=255)         cTGGTCAATACGCTGAAGACCGCATCAATGCCATGCTGATGTACAACGCCAACATCATCGGTCAGGCTACCCGCAATGCCAATCACCGGTTTATGGTACTTCTTCGCCACGTTTGCGACACCAATCGTTACCTTCCCGTg                                                                                                                          >  1:68365/1‑140 (MQ=255)         cTGGTCAATACGCTGAAGACCGCATCAATGCCATGCTGATGTACAACGCCAACATCATCGGTCAGGCTACCCGCAATGCCAATCACCGGTTTATGGTACTTCTTCGCCACGTTTGCGACACCAATCGTTACCTTCCCGTg                                                                                                                          >  1:27806/1‑140 (MQ=255)           ggTCAATACGCTGAAGACCGCATCAATGCCATGCTGATGTACAACGCCAACATCATCGGTCAGGCTACCCGCAATGCCAATCACCGGTTTATGGTACTTCTTCGCCACGTTTGCGACACCAATCGTTACCTTCCCGTGaa                                                                                                                        >  2:11346/1‑140 (MQ=255)            gTCAATACGCTGAAGACCGCATCAATGCCATGCTGATGTACAACGCCAACATCATCGGTCAGGCTACCCGCAATGCCAATCACCGGTTTATGGTACTTCTTCGCCACGTTTGCGACACCAATCGTTACCtt                                                                                                                                <  2:25635/131‑1 (MQ=255)            gTCAATACGCTGAAGACCGCATCAATGCCATGCTGATGTACAACGCCAACATCATCGGTCAGGCTACCCGCAATGCCAATCACCGGTTTATGGTACTTCTTCGCCACGTTTGCGACACCAATCGTTACCtt                                                                                                                                >  1:25635/1‑131 (MQ=255)            gTCAATACGCTGAAGACCGCATCAATGCCATGCTGATGTACAACGCCAACATCATCGGTCAGGCTACCCGCAATGCCAATCACCGGTTTATGGTACTTCTTCGCCACGTTTGCGACACCAATCGTTACCTTCCCGTGAAt                                                                                                                       >  1:146948/1‑140 (MQ=255)            gTCAATACGCTGAAGACCGCATCAATGCCATGCTGATGTACAACGCCAACATCATCGGTCAGGCTACCCGCAATGCCAATCACCGGTTTATGGTACTTCTTCGCCACGTTTGCGACACCAATCGTTACCTTCCCGTGAAt                                                                                                                       >  2:12959/1‑140 (MQ=255)            gTCAATACGCTGAAGACCGCATCAATGCCATGCTGATGTACAACGCCAACATCATCGGTCAGGCTACCCGCAATGCCAATCACCGGTTTATGGTACTTCTTCGCCACGTTTGCGACACCAATCGTTACCTTCCCGTGAAt                                                                                                                       >  1:51629/1‑140 (MQ=255)                aTACGCTGAAGACCGCATCAATGCCATGCTGATGTACAACGCCAACATCATCGGTCAGGCTACCCGCAATGCCAATCACCGGTTTATGGTACTTCTTCGCCACGTTTGCGACACCAATCGTTACCTTCCCGTGAATActc                                                                                                                   <  2:298706/140‑1 (MQ=255)                 tACGCTGAAGACCGCATCAATGCCATGCTGATGTACAACGCCAACATCATCGGTCAGGCTACCCGCAATGCCAATCACCGGTTTATGGTACTTCTTCGCCACGTTTGCGACACCAATCGTTACCTTCCCGTGAATActct                                                                                                                  >  1:261446/1‑140 (MQ=255)                        aaGACCGCATCAATGCCATGCTGATGTACAACGCCAACATCATCGGTCAGGCTACCCGCAATGCCAATCACCGGTTTATGGTACTTCTTCGCCACGTTTGCGACACCAATCGTTACCTTCCCGTGAATAct                                                                                                                    >  1:113337/1‑131 (MQ=255)                        aaGACCGCATCAATGCCATGCTGATGTACAACGCCAACATCATCGGTCAGGCTACCCGCAATGCCAATCACCGGTTTATGGTACTTCTTCGCCACGTTTGCGACACCAATCGTTACCTTCCCGTGAATAct                                                                                                                    <  2:113337/131‑1 (MQ=255)                           aCCGCATCAATGCCATGCTGATGTACAACGCCAACATCATCGGTCAGGCTACCCGCAATGCCAATCACCGGTTTATGGTACTTCTTCGCCACGTTTGCGACACCAATCGTTACCTTCCCGTGAATACTCTGGCTGTCAAt                                                                                                        <  1:11822/140‑1 (MQ=255)                             cGCATCAATGCCATGCTGATGTACAACGCCAACATCATCGGTCAGGCTACCCGCAATGCCAATCACCGGTTTATGGTACTTCTTCGCCACGTTTGCGACACCAATCGTTACCTTCCCGTGAATACTCTGGCTGTCAATAc                                                                                                      >  2:36042/1‑140 (MQ=255)                              gCATCAATGCCATGCTGATGTACAACGCCAACATCATCGGTCAGGCTACCCGCAATGCCAATCACCGGTTTATGGTACTTCTTCGCCACGTTTGCGACACCAATCGTTACCTTCCCGTGAATACTCTGGCTGTCAATACg                                                                                                     <  2:311670/140‑1 (MQ=255)                              gCATCAATGCCATGCTGATGTACAACGCCAACATCATCGGTCAGGCTACCCGCAATGCCAATCACCGGTTTATGGTACTTCTTCGCCACGTTTGCGACACCAATCGTTACCTTCCCGTGAATACTCTGGCTGTCAATACg                                                                                                     >  1:258535/1‑140 (MQ=255)                              gCATCAATGCCATGCTGATGTACAACGCCAACATCATCGGTCAGGCTACCCGCAATGCCAATCACCGGTTTATGGTACTTCTTCGCCACGTTTGCGACACCAATCGTTACCTTCCCGTGAATACTCTGGCTGTCAATACg                                                                                                     <  2:146948/140‑1 (MQ=255)                                 tCAATGCCATGCTGATGTACAACGCCAACATCATCGGTCAGGCTACCCGCAATGCCAATCACCGGTTTATGGTACTTCTTCGCCACGTTTGCGACACCAATCGTTACCTTCCCGTGAATACTCTGGCTGTCAATACGccc                                                                                                  <  1:30583/140‑1 (MQ=255)                                   aaggCCATGTTGATGTACAACGTCAACATCATGGGTCAGGCTACCCGCAATGCCAATCCCCGGTTTATGGTACTGCTTCGCCACGTTTGCGACACCAATCGTTACCTTCCCGTGAATACTCTGGCTGTCAATACGCCCtt                                                                                                <  2:78197/137‑1 (MQ=255)                                      gCCATGCTGATGTACAACGCCAACATCATCGGTCAGGCTACCCGCAATGCCAATCACCGGTTTATGGTACTTCTTCGCCACGTTTGCGACACCAATCGTTACCTTCCCGTGAATACTCTGGCTGTCAATACGCCCTTCAc                                                                                             <  2:60152/140‑1 (MQ=255)                                           gCTGATGTACAACGCCAACATCATCGGTCAGGCTACCCGCAATGCCAATCACCGGTTTATGGTACTTCTTCGCCACGTTTGCGACACCAATCGTTACCTTCCCGTGAATACTCTGGCTGTCAATACGCCCTTCACCGGTg                                                                                        <  1:357581/140‑1 (MQ=255)                                             tGATGTACAACGCCAACATCATCGGTCAGGCTACCCGCAATGCCAATCACCGGTTTATGGTACTTCTTCGCCACGTTTGCGACACCAATCGTTACCTTCCCGTGAATACTCTGGCTGTCAATACGCCCTTCACCGGTGAt                                                                                      <  2:261446/140‑1 (MQ=255)                                                      aCGCCAACATCATCGGTCAGGCTACCCGCAATGCCAATCACCGGTTTATGGTACTTCTTCGCCACGTTTGCGACACCAATCGTTACCTTCCCGTGAATACTCTGGCTGTCAATACGCCCTTCACCGGTGAt                                                                                      >  2:274553/1‑131 (MQ=255)                                                      aCGCCAACATCATCGGTCAGGCTACCCGCAATGCCAATCACCGGTTTATGGTACTTCTTCGCCACGTTTGCGACACCAATCGTTACCTTCCCGTGAATACTCTGGCTGTCAATACGCCCTTCACCGGTGAt                                                                                      <  1:274553/131‑1 (MQ=255)                                                       cGCCAACATCATCGGTCAGGCTACCCGCAATGCCAATCACCGGTTTATGGTACTTCTTCGCCACGTTTGCGACACCAATCGTTACCTTCCCGTGAATACTCTGGCTGTCAATACGCCCTTCACCGGTGATCACCAGCGTa                                                                            <  2:58087/140‑1 (MQ=255)                                                       cGCCAACATCATCGGTCAGGCTACCCGCAATGCCAATCACCGGTTTATGGTACTTCTTCGCCACGTTTGCGACACCAATCGTTACCTTCCCGTGAATACTCTGGCTGTCAATACGCCCTTCACCGGTGATCACCAGCGTa                                                                            <  2:400518/140‑1 (MQ=255)                                                       cGCCAACATCATCGGTCAGGCTACCCGCAATGCCAATCACCGGTTTATGGTACTTCTTCGCCACGTTTGCGACACCAATCGTTACCTTCCCGTGAATACTCTGGCTGTCAATACGCCCTTCACCGGTGATCACCAGCGTa                                                                            >  1:58087/1‑140 (MQ=255)                                                         ccAACATCATCGGTCAGGCTACCCGCAATGCCAATCACCGGTTTATGGTACTTCTTCGCCACGTTTGCGACACCAATCGTTACCTTCCCGTGAATACTCTGGCTGTCAATACGCCCTTCACCGGTGATCACCAGCGTACa                                                                          <  2:47694/140‑1 (MQ=255)                                                         ccAACATCATCGGTCAGGCTACCCGCAATGCCAATCACCGGTTTATGGTACTTCTTCGCCACGTTTGCGACACCAATCGTTACCTTCCCGTGAATACTCTGGCTGTCAATACGCCCTTCACCGGTGATCACCAGCGTACa                                                                          >  1:136729/1‑140 (MQ=255)                                                             catcatCGGTCAGGCTACCCGCAATGCCAATCACCGGTTTATGGTACTTCTTCGCCACGTTTGCGACACCAATCGTTACCTTCCCGTGAATACTCTGGCTGTCAATACGCCCTTCACCGGTGATCACCAGCGTACAATCg                                                                      <  2:27806/140‑1 (MQ=255)                                                                catcGGTCAGGCTACCCGCAATGCCAATCACCGGTTTATGGTACTTCTTCGCCACGTTTGCGACACCAATCGTTACCTTCCCGTGAATACTCTGGCTGTCAATACGCCCTTCACCGGTGATCACCAGCGTACAATCGTGa                                                                   <  1:249126/140‑1 (MQ=255)                                                                       cAGGCTACCCGCAATGCCAATCACCGGTTTATGGTACTTCTTCGCCACGTTTGCGACACCAATCGTTACCTTCCCGTGAATACTCTGGCTGTCAATACGCCCTTCACCGGTGATCACCAGCGTACAATCGTGAATATGtt                                                            <  1:12959/140‑1 (MQ=255)                                                                       cAGGCTACCCGCAATGCCAATCACCGGTTTATGGTACTTCTTCGCCACGTTTGCGACACCAATCGTTACCTTCCCGTGAATACTCTGGCTGTCAATACGCCCTTCACCGGTGATCACCAGCGTACAATCGTGAATATGtt                                                            <  2:258535/140‑1 (MQ=255)                                                                                    aTGCCAATCACCGGTTTATGGTACTTCTTCGCCACGTTTGCGACACCAATCGTTACCTTCCCGTGAATACTCTGGCTGTCAATACGCCCTTCACCGGTGATCACCAGCGTACAATCGTGAATATGTTCCTCCAGATTCAg                                               >  1:354407/1‑140 (MQ=255)                                                                                            cACCGGTTTATGGTACTTCTTCGCCACGTTTGCGACACCAATCGTTACCTTCCCGTGAATACTCTGGCTGTCAATACGCCCTTCACCGGTGATCACCAGCGTACAATCGTGAATATGTTCCTCCAGATTCAGCGCCGTAg                                       >  1:255038/1‑140 (MQ=255)                                                                                                        gTACTTCTTCGCCACGTTTGCGACACCAATCGTTACCTTCCCGTGAATACTCTGGCTGTCAATACGCCCTTCACCGGTGATCACCAGCGTACAATCGTGAATATGTTCCTCCAGATTCAGCGCCGTAGTGACGATTTCaa                           <  2:354407/140‑1 (MQ=255)                                                                                                                tcGCCACGTTTGCGACACCAATCGTTACCTTCCCGTGAATACTCTGGCTGTCAATACGCCCTTCACCGGTGATCACCAGCGTACAATCGTGAATATGTTCCTCCAGATTCAGCGCCGTAGTGACGATTTCAATACCACtt                   >  1:153115/1‑140 (MQ=255)                                                                                                                        tttGCGACACCAATCGTTACCTTCCCGTGAATACTCTGGCTGTCAATACGCCCTTCACCGGTGATCACCAGCGTACAATCGTGAATATGTTCCTCCAGATTCAGCGCCGTAGTGACGATTTCAATACCACTTTTCAGTTc           >  1:282356/1‑140 (MQ=255)                                                                                                                                 ccAATCGTTACCTTCCCGTGAATACTCTGGCTGTCAATACGCCCTTCACCGGTGATCACCAGCGTACAATCGTGAATATGTTCCTCCAGATTCAGCGCCGTAGTGACGATTTCAATACCACTTTTCAGTTCCGCACCAAg  >  1:236481/1‑140 (MQ=255)                                                                                                                                        |                                                                                                                                     TACCTATGCTGGTCAATACGCTGAAGACCGCATCAATGCCATGCTGATGTACAACGCCAACATCATCGGTCAGGCTACCCGCAATGCCAATCACCGGTTTATGGTACTTCTTCGCCACGTTTGCGACACCAATCGGTACCTTCCCGTGAATACTCTGGCTGTCAATACGCCCTTCACCGGTGATCACCAGCGTACAATCGTGAATATGTTCCTCCAGATTCAGCGCCGTAGTGACGATTTCAATACCACTTTTCAGTTCCGCACCAAG  >  NC_000913/3270723‑3270990

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑