Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,416,802	G→T	L590F (TTG→TTT)	acrF →	multidrug efflux pump RND permease AcrF

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,416,802	0	G	T	100.0%	122.9 / NA	36	L590F (TTG→TTT)	acrF	multidrug efflux pump RND permease AcrF
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (16/20);  total (16/20)

GCTGATTGTTGCAGGAATGGTGGTGTTGTTTTTACGTCTTCCGTCTTCCTTCTTACCTGAAGAGGATCAGGGTGTCTTTCTGACCATGATTCAGTTACCCGCTGGCGCGACGCAAGAGCGGACGCAAAAAGTGTTGGATCAAGTTACGGATTACTATCTGAAGAACGAGAAAGCGAACGTTGAAAGTGTCTTTACGGTTAACGGCTTTAGCTTCAGCGGCCAGGCACAAAACGCCGGTATGGCCTTCGTCAGTCTGAAACCGTGGGAA  >  NC_000913/3416667‑3416934                                                                                                                                        |                                                                                                                                     gcTGATTGTTGCAGGAATGGTGGTGTTGTTTTTACGTCTTCCGTCTTCCTTCTTACCTGAAGAGGATCAGGGTGTCTTTCTGACCATGATTCAGTTACCCGCTGGCGCGACGCAAGAGCGGACGCAAAAAGTGTTTGATc                                                                                                                                  <  2:271874/140‑1 (MQ=255)   tGATTGTTGCAGGAATGGTGGTGTTGTTTTTACGTCTTCCGTCTTCCTTCTTACCTGAAGAGGATCAGGGTGTCTTTCTGACCATGATTAAGTTACCCGCTGGCGCGACGCAAGAGCGGACGCAAAAAGTGTTTGATCaa                                                                                                                                >  1:239642/1‑140 (MQ=255)    gATTGTTGCAGGAATGGTGGTGTTGTTTTTACGTCTTCCGTCTTCCTTCTTACCTGAAGAGGATCAGGGTGTCTTTCTGACCATGATTCAGTTACCCGCTGGCGCGACGCAAGAGCGGACGCAAAAAGTGTTTGATCAAg                                                                                                                               <  2:176637/140‑1 (MQ=255)     aTTGTTGCAGGAATGGTGGTGTTGTTTTTACGTCTTCCGTCTTCCTTCTTACCTGAAGAGGATCAGGGTGTCTTTCTGACCATGATTCAGTTACCCGCTGGCGCGACGCAAGAGCGGACGCAAAAAGTGTTTGATCAAGt                                                                                                                              >  2:382992/1‑140 (MQ=255)     aTTGTTGCAGGAATGGTGGTGTTGTTTTTACGTCTTCCGTCTTCCTTCTTACCTGAAGAGGATCAGGGTGTCTTTCTGACCATGATTCAGTTACCCGCTGGCGCGACGCAAGAGCGGACGCAAAAAGTGTTTGATCAAGt                                                                                                                              >  2:112307/1‑140 (MQ=255)     aTTGTTGCAGGAATGGTGGTGTTGTTTTTACGTCTTCCGTCTTCCTTCTTACCTGAAGAGGATCAGGGTGTCTTTCTGACCATGATTCAGTTACCCGCTGGCGCGACGCAAGAGCGGACGCAAAAAGTGTTTGATCAAGt                                                                                                                              <  2:333039/140‑1 (MQ=255)     aTTGTTGCAGGAATGGTGGTGTTGTTTTTACGTCTTCCGTCTTCCTTCTTACCTGAAGAGGATCAGGGTGTCTTTCTGACCATGATTCAGTTACCCGCTGGCGCGACGCAAGAGCGGACGCAAAAAGTGTTTGATCAAGt                                                                                                                              >  2:351325/1‑140 (MQ=255)     aTTGTTGCAGGAATGGTGGTGTTGTTTTTACGTCTTCCGTCTTCCTTCTTACCTGAAGAGGATCAGGGTGTCTTTCTGACCATGATTCAGTTACCCGCTGGCGCGACGCAAGAGCGGACGCAAAAAGTGTTTGATCAAGt                                                                                                                              >  1:357103/1‑140 (MQ=255)     aTTGTTGCAGGAATGGTGGTGTTGTTTTTACGTCTTCCGTCTTCCTTCTTACCTGAAGAGGATCAGGGTGTCTTTCTGACCATGATTCAGTTACCCGCTGGCGCGACGCAAGAGCGGACGCAAAAAGTGTTTGATCAAGt                                                                                                                              >  2:48301/1‑140 (MQ=255)     aTTGTTGCAGGAATGGTGGTGTTGTTTTTACGTCTTCCGTCTTCCTTCTTACCTGAAGAGGATCAGGGTGTCTTTCTGACCATGATTCAGTTACCCGCTGGCGCGACGCAAGAGCGGACGCAAAAAGTGTTTGATCAAGt                                                                                                                              >  1:124037/1‑140 (MQ=255)      ttgttgCAGGAATGGTGGTGTTGTTTTTACGTCTTCCGTCTTCCTTCTTACCTGAAGAGGATCAGGGTGTCTTTCTGACCATGATTCAGTTACCCGCTGGCGCGACGCAAGAGCGGACGCAAAAAGTGTTTGATCAAGtt                                                                                                                             <  1:382992/140‑1 (MQ=255)      ttgttgCAGGAATGGTGGTGTTGTTTTTACGTCTTCCGTCTTCCTTCTTACCTGAAGAGGATCAGGGTGTCTTTCTGACCATGATTCAGTTACCCGCTGGCGCGACGCAAGAGCGGACGCAAAAAGTGTTTGATCAAGtt                                                                                                                             <  1:219478/140‑1 (MQ=255)       tgttgCAGGAATGGTGGTGTTGTTTTTACGTCTTCCGTCTTCCTTCTTACCTGAAGAGGATCAGGGTGTCTTTCTGACCATGATTCAGTTACCCGCTGGCGCGACGCAAGAGCGGACGCAAAAAGTGTTTGATCAAGTTa                                                                                                                            <  2:398325/140‑1 (MQ=255)           gCAGGAATGGTGGTGTTGTTTTTACGTCTTCCGTCTTCCTTCTTACCTGAAGAGGATCAGGGTGTCTTTCTGACCATGATTCAGTTACCCGCTGGCGCGACGCAAGAGCGGACGCAAAAAGTGTTTGATCAAGTTACGGa                                                                                                                        <  1:48301/140‑1 (MQ=255)               gAATGGTGGTGTTGTTTTTACGTCTTCCGTCTTCTTTCTTACCTGAAGAGGATCAGGGTGTCTTTCTGACCATGATTCAGTTACCCGCTGGCGCGACGCAAGAGCGGACGCAAAAAGTGTTTGAt                                                                                                                                   <  1:401848/125‑1 (MQ=255)               gAATGGTGGTGTTGTTTTTACGTCTTCCGTCTTCTTTCTTACCTGAAGAGGATCAGGGTGTCTTTCTGACCATGATTCAGTTACCCGCTGGCGCGACGCAAGAGCGGACGCAAAAAGTGTTTGAt                                                                                                                                   >  2:401848/1‑125 (MQ=255)                                    gtcttccgtcttccTTCTTACCTGAAGAGGATCAGGGTGTCTTTCTGACCATGATTCAGTTACCCGCTGGCGCGACGCAAGAGCGGACGCAAAAAGTGTTTGATCAAGTTACGGATTACTATCTGAAGAACGAGAAAGCg                                                                                               <  2:313031/140‑1 (MQ=255)                                      cttccgtcttccTTCTTACCTGAAGAGGATCAGGGTGTCTTTCTGACCATGATTAAGTTACCCGCTGGCGCGACGCAAGAGCGGACGCAAAAAGTGTTTGATCAAGTTACGGATTACTATCTGAAGAACGAGAAAGCGaa                                                                                             <  2:239642/140‑1 (MQ=255)                                                                ggATCAGGGTGTCTTTCTGACCATGATTCAGTTACCCGCTGGCGCGACGCAAGAGCGGACGCAAAAAGTGTTTGATCAAGTTACGGATTACTATCTGAAGAACGAGAAAGCGAACGTTGAAAGTGTCTTTACGGTTAACg                                                                   <  1:112307/140‑1 (MQ=255)                                                                   tCAGGGTGTCTTTCTGACCATGATTCAGTTACCCGCTGGCGCGACGCAAGAGCGGACGCAAAAAGTGTTTGATCAAGTTACGGATTACTATCTGAAGAACGAGAAAGCGAACGTTGAAAGTGTCTTTACGGTTaa                                                                     <  2:71026/135‑1 (MQ=255)                                                                   tCAGGGTGTCTTTCTGACCATGATTCAGTTACCCGCTGGCGCGACGCAAGAGCGGACGCAAAAAGTGTTTGATCAAGTTACGGATTACTATCTGAAGAACGAGAAAGCGAACGTTGAAAGTGTCTTTACGGTTaa                                                                     >  1:71026/1‑135 (MQ=255)                                                                           tCTTTCTGACCATGATTCAGTTACCCGCTGGCGCGACGCAAGAGCGGACGCAAAAAGTGTTTGATCAAGTTACGGATTACTATCTGAAGAACGAGAAAGCGAACGTTGAAAGTGTCTTTACGGTTAACGGCTTTAGCTTc                                                        >  1:356846/1‑140 (MQ=255)                                                                            cTTTCTGACCATGATTCAGTTACCCGCTGGCGCGACGCAAGAGCGGACGCAAAAAGTGTTTGATCAAGTTACGGATTACTATCTGAAGAACGAGAAAGCGAACGTTGAAAGTGTCTTTACGGTTAACGGCTTTAGCTTCa                                                       >  1:185732/1‑140 (MQ=255)                                                                                     cATGATTCAGTTACCCGCTGGCGCGACGCAAGAGCGGACGCAAAAAGTGTTTGATCAAGTTACGGATTACTATCTGAAGAACGAGAAAGCGAACGTTGAAAGTGTCTTTACGGTTAACGGCTTTAGCTTCAGCGGCCAgg                                              >  2:411773/1‑140 (MQ=255)                                                                                       tGATTCAGTTACCCGCTGCCGCGACGCAAGAGCGGCCGCAAAAAGTGTTTGATCAAGTTACGGATTACTATCTGAAGAACGAGAAAGCGAACGTTGAAAGTGTCTTTACGGTTAACGGCTTTAGCTTCAGCGACCAGGca                                            >  2:131650/1‑140 (MQ=255)                                                                                              gTTACCCGCTGGCGCGACGCAAGAGCGGACGCAAAAAGTGTTTGATCAAGTTACGGATTACTATCTGAAGAACGAGAAAGCGAACGTTGAAAGTGTCTTTACGGTTAACGGCTTTAGCTTCAGCGGCCAGGCACAAAACg                                     <  2:317648/140‑1 (MQ=255)                                                                                              gTTACCCGCTGGCGCGACGCAAGAGCGGACGCAAAAAGTGTTTGATCAAGTTACGGATTACTATCTGAAGAACGAGAAAGCGAACGTTGAAAGTGTCTTTACGGTTAACGGCTTTAGCTTCAGCGGCCAGGCACAAAACg                                     <  2:185732/140‑1 (MQ=255)                                                                                                  cccGCTGGCGCGACGCAAGAGCGGACGCAAAAAGTGTTTGATCAAGTTACGGATTACTATCTGAAGAACGAGAAAGCGAACGTTGAAAGTGTCTTTACGGTTAACGGCTTTAGCTTCAGCGGCCAGGCACAAAACGCCgg                                 >  2:298724/1‑140 (MQ=255)                                                                                                  cccGCTGGCGCGACGCAAGAGCGGACGCAAAAAGTGTTTGATCAAGTTACGGATTACTATCTGAAGAACGAGAAAGCGAACGTTGAAAGTGTCTTTACGGTTAACCGCTTTAGCTTCAGCGGCCAGGCACAAAACGCCgg                                 >  2:142414/1‑140 (MQ=255)                                                                                                      cTGGCGCGACGCAAGAGCGGACGCAAAAAGTGTTTGATCAAGTTACGGATTACTATCTGAAGAACGAGAAAGCGAACGTTGAAAGTGTCTTTACGGTTAACGGCTTTAGCTTCAGCGGCCAGGCACAAAACGCCGGTATg                             <  1:94255/140‑1 (MQ=255)                                                                                                              aCGCAAGAGCGGACGCAAAAAGTGTTTGATCAAGTTACGGATTACTATCTGAAGAACGAGAAAGCGAACGTTGAAAGTGTCTTTACGGTTAACGGCTTTAGCTTCAGCGGCCAGGCACAAAACGCCGGTATGGCCTTCGt                     <  2:30822/140‑1 (MQ=255)                                                                                                               cGCAAGAGCGGACGCAAAAAGTGTTTGATCAAGTTACGGATTACTATCTGAAGAACGAGAAAGCGAACGTTGAAAGTGTCTTTACGGTTAACGGCTTTAGCTTCAGCGGCCAGGCACAAAACGCCGGTATGGCCTTCGTc                    <  1:298724/140‑1 (MQ=255)                                                                                                                        ggACGCAAAAAGTGTTTGATCAAGTTACGGATTACTATCTGAAGAACGAGAAAGCGAACGTTGAAAGTGTCTTTACGGTTAACGGCTTTAGCTTCAGCGGCCAGGCACAAAACGCCGGTATGGCCTTCGTCAGTCTGaaa           >  1:400745/1‑140 (MQ=255)                                                                                                                            gCAAAAAGTGTTTGATCAAGTTACGGATTACTATCTGAAGAACGAGAAAGCGAACGTTGAAAGTGTCTTTACGGTTAACGGCTTTAGCTTCAGCGGCCAGGCACAAAACGCCGGTATGGCCTTCGTCAGTCTGAAACCGt       >  1:369590/1‑140 (MQ=255)                                                                                                                               aaaaGTGTTTGATCAAGTTACGGATTACTATCTGAAGAACGAGAAAGCGAACGTTGAAAGTGTCTTTACGGTTAACGGCTTTAGCTTCAGCGGCCAGGCACAAAACGCCGGTATGGCCTTCGTCAGTCTGAAACCGTggg    <  2:124037/140‑1 (MQ=255)                                                                                                                               aaaaGTGTTTGATCAAGTTACGGATTACTATCTGAAGAACGAGAAAGCGAACGTTGAAAGTGTCTTTACGGTTAACGGCTTTAGCTTCAGCGGCCAGGCACAAAACGCCGGTATGGCCTTCGTCAGTCTGAAACCGTggg    <  2:357103/140‑1 (MQ=255)                                                                                                                                 aaGTGTTTGATCAAGTTACGGATTACTATCTGAAGAACGAGAAAGCGAACGTTGAAAGTGTCTTTACGGTTAACGGCTTTAGCTTCAGCGGCCAGGCACAAAACGCCGGTATGGCCTTCGTCAGTCTGAAACCGTGGGaa  <  2:400745/140‑1 (MQ=255)                                                                                                                                        |                                                                                                                                     GCTGATTGTTGCAGGAATGGTGGTGTTGTTTTTACGTCTTCCGTCTTCCTTCTTACCTGAAGAGGATCAGGGTGTCTTTCTGACCATGATTCAGTTACCCGCTGGCGCGACGCAAGAGCGGACGCAAAAAGTGTTGGATCAAGTTACGGATTACTATCTGAAGAACGAGAAAGCGAACGTTGAAAGTGTCTTTACGGTTAACGGCTTTAGCTTCAGCGGCCAGGCACAAAACGCCGGTATGGCCTTCGTCAGTCTGAAACCGTGGGAA  >  NC_000913/3416667‑3416934

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑