Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,459,294	A→G	E319G (GAA→GGA)	gspE →	Type II secretion system protein GspE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,459,294	0	A	G	100.0%	76.7 / NA	22	E319G (GAA→GGA)	gspE	Type II secretion system protein GspE
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (13/9);  total (13/9)

CGGTAGAAGATCCCGTGGAATATGAGCTGGAAGGCATTGGGCAAACGCAGGTGAATACCCGTGTGGATATGTCTTTCGCTCGCGGCCTGCGCGCCATACTTCGCCAGGACCCGGATGTCGTCATGGTGGGGGAAATTCGTGATACAGAAACCGCGCAGATTGCGGTTCAGGCCTCGCTCACCGGCCATCTGGTACTCTCAACACTCCACACTAACAGTGCATCAGGCGCAGTGACCCGGCTCCGCGACATGGGCGTCGAATCAT  >  NC_000913/3459162‑3459425                                                                                                                                     |                                                                                                                                    cGGTAGAAGATCCCGTGGAATATGAGCTGGAAGGCATTGGGCAAACGCAGGTGAATACCCGTGTGGATATGTCTTTCGCTCGCGGCCTGCGCGCCATACTTCGCCAGGACCCGGATGTCGTCATGGTGGGGGGAATTCGt                                                                                                                              <  2:176104/140‑1 (MQ=255)   gTAGAAGATCCCGTGGAATATGAGCTGGAAGGCATTGGGCAAACGCAGGTGAATACCCGTGTGGATATGTCTTTCGCTCGCGGCCTGCGCGCCATACTTCGCCAGGACCCGGATGTCGTCATGGTGGGGGGAATTCGTGa                                                                                                                            <  1:382532/140‑1 (MQ=255)     agaagaTCCCGTGGAATATGAGCTGGAAGGCATTGGGCAAACGCAGGTGAATACCCGTGTGGATATGTCTTTCGCTCGCGGCCTGCGCGCCATACTTCGCCAGGACCCGGATGTCGTCATGGTGGGGGGAATTCGTGATa                                                                                                                          >  1:154247/1‑140 (MQ=255)                                               gCAGGTGAATACCCGTGTGGATATGTCTTTCGCTCGCGGCCTGCGCGCCATACTTCGCCAGGACCCGGATGTCGTCATGGTGGGGGGAATTCGTGATACAGAAACCGCGCAGATTGCGGTTCAGGCCTCGCTCACCGGcc                                                                                <  2:205732/140‑1 (MQ=255)                                                             gtgtGGATATGTCTTTCGCTCGCGGCCTGCGCGCCATACTTCGCCAGGACCCGGATGTCGTCATGGTGGGGGGAATTCGTGATACAGAAACCGCGCAGATTGCGGTTCAGGCCTCGCTCACCGGcc                                                                                >  1:188487/1‑126 (MQ=255)                                                             gtgtGGATATGTCTTTCGCTCGCGGCCTGCGCGCCATACTTCGCCAGGACCCGGATGTCGTCATGGTGGGGGGAATTCGTGATACAGAAACCGCGCAGATTGCGGTTCAGGCCTCGCTCACCGGcc                                                                                <  2:188487/126‑1 (MQ=255)                                                             gtgtGGATATGTCTTTCGCTCGCGGCCTGCGCGCCATACTTCGCCAGGACCCGGATGTCGTCATGGTGGGGGGAATTCGTGATACAGAAACCGCGCAGATTGCGGTTCAGGCCTCGCTCACCGGCCATCTGGTACTCTCa                                                                  >  2:302684/1‑140 (MQ=255)                                                                  gATATGTCTTTCGCTCGCGGCCTGCGCGCCATACTTCGCCAGGACCCGGATGTCGTCATGGTGGGGGGAATTCGTGATACAGAAACCGCGCAGATTGCGGTTCAGGCCTCGCTCACCGGCCATCTGGTACTCTCAACACt                                                             >  2:79240/1‑140 (MQ=255)                                                                            tcgctcgcGGCCTGCGCGCCATACTTCGCCAGGACCCGGATGTCGTCATGGTGGGGGGAATTCGTGATACAGAAACCGCGCAGATTGCGGTTCAGGCCTCGCTCACCGGCCATCTGGTACTCTCAACACTCCACACTAAc                                                   >  2:9859/1‑140 (MQ=255)                                                                             cgctcgcGGCCTGCGCGCCATACTTCGCCAGGACCCGGATGTCGTCATGGTGGGGGGAATTCGTGATACAGAAACCGCGCAGATTGCGGTTCAGGCCTCGCTCACCGGCCATCTGGTACTCTCAACACTCCACACTAACa                                                  <  1:9859/140‑1 (MQ=255)                                                                                 cgcgGCCTGCGCGCCATACTTCGCCAGGACCCGGATGTCGTCATGGTGGGGGGAATTCGTGATACAGAAACCGCGCAGATTGCGGTTCAGGCCTCGCTCACCGGCCATCTGGTACTCTCAACACTCCACACTAACAGTGc                                              <  1:302684/140‑1 (MQ=255)                                                                                  gcgGCCTGCGCGCCATACTTCGCCAGGACCCGGATGTCGTCATGGTGGGGGGAATTCGTGATACAGAAACCGCGCAGATTGCGGTTCAGGCCTCGCTCACCGGCCATCTGGTACTCTCAACACTCCACACTAACAGTGCa                                             >  1:254932/1‑140 (MQ=255)                                                                                        tGCGCGCCATACTTCGCCAGGACCCGGATGTCGTCATGGTGGGGGGAATTCGTGATACAGAAACCGCGCAGATTGCGGTTCAGGCCTCGCTCACCGGCCATCTGGTACTCTCAACACTCCACACTAACAGTGCATCAGgc                                       >  2:102575/1‑140 (MQ=255)                                                                                          cgcgcCATACTTCGCCAGGACCCGGATGTCGTCATGGTGGGGGGAATTCGTGATACAGAAACCGCGCAGATTGCGGTTCAGGCCTCGCTCACCGGCCATCTGGTACTCTCAACACTCCACACTAACAGTGc                                              >  2:154598/1‑131 (MQ=255)                                                                                          cgcgcCATACTTCGCCAGGACCCGGATGTCGTCATGGTGGGGGGAATTCGTGATACAGAAACCGCGCAGATTGCGGTTCAGGCCTCGCTCACCGGCCATCTGGTACTCTCAACACTCCACACTAACAGTGc                                              <  1:154598/131‑1 (MQ=255)                                                                                            cgcCATACTTCGCCAGGACCCGGATGTCGTCATGGTGGGGGGAATTCGTGATACAGAAACCGCGCAGATTGCGGTTCAGGCCTCGCTCACCGGCCATCTGGTACTCTCAACACTCCACACTAACAGTGCATCAGGCGCAg                                   <  1:79240/140‑1 (MQ=255)                                                                                                         cAGGACCCGGATGTCGTCATGGTGGGGGGAATTCGTGATACAGAAACCGCGCAGATTGCGGTTCAGGCCTCGCTCACCGGCCATCTGGTACTCTCAACACTCCACACTAACAGTGCATCAGGCGCAGTGACCCGGCTCcg                      >  2:338622/1‑140 (MQ=255)                                                                                                                      tcgtcATGGTGGGGGGAATTCGTGATACAGAAACCGCGCAGATTGCGGTTCAGGCCTCGCTCACCGGCCATCTGGTACTCTCAACACTCCACACTAACAGTGCATCAGGCGCAGTGACCCGGCTCCGCGACATGGGCGTc         >  1:374110/1‑140 (MQ=255)                                                                                                                       cgtcATGGTGGGGGGAATTCGTGATACAGAAACCGCGCAGATTGCGGTTCAGGCCTCGCTCACCGGCCATCTGGTGCTCTCAACACTCCACACTAACAGTGCATCAGGCGCAGTGACCCGGCTc                        <  1:351364/124‑1 (MQ=255)                                                                                                                       cgtcATGGTGGGGGGAATTCGTGATACAGAAACCGCGCAGATTGCGGTTCAGGCCTCGCTCACCGGCCATCTGGTACTCTCAACACTCCACACTAACAGTGCATCAGGCGCAGTGACCCGGCTc                        >  2:351364/1‑124 (MQ=255)                                                                                                                             ggtggGGGGAATTCGTGATACAGAAACCGCGCAGATTGCGGTTCAGGCCTCGCTCACCGGCCATCTGGTACTCTCAACACTCCACACTAACAGTGCATCAGGCGCAGTGACCCGGCTCCGCGACATGGGCGTCGAATCAt  >  2:250927/1‑140 (MQ=255)                                                                                                                             ggtggGGGGAATTCGTGATACAGAAACCGCGCAGATTGCGGTTCAGGCCTCGCTCACCGGCCATCTGGTACTCTCAACACTCCACACTAACAGTGCATCAGGCGCAGTGACCCGGCTCCGCGACATGGGCGTCGAATCAt  >  1:239449/1‑140 (MQ=255)                                                                                                                                     |                                                                                                                                    CGGTAGAAGATCCCGTGGAATATGAGCTGGAAGGCATTGGGCAAACGCAGGTGAATACCCGTGTGGATATGTCTTTCGCTCGCGGCCTGCGCGCCATACTTCGCCAGGACCCGGATGTCGTCATGGTGGGGGAAATTCGTGATACAGAAACCGCGCAGATTGCGGTTCAGGCCTCGCTCACCGGCCATCTGGTACTCTCAACACTCCACACTAACAGTGCATCAGGCGCAGTGACCCGGCTCCGCGACATGGGCGTCGAATCAT  >  NC_000913/3459162‑3459425

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑