Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,834,980	G→T	A317D (GCC→GAC)	yicJ ←	putative xyloside transporter YicJ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,834,980	0	G	T	100.0%	91.8 / NA	27	A317D (GCC→GAC)	yicJ	putative xyloside transporter YicJ
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (16/11);  total (16/11)

GATCCCGGTCAGGCGTTTACCATTGCACCACTCGCCGTAGTCGACGGTATCGGACATCATTACCCACTGGATAGGTGTCACCAGTTGATGCAACACACCAATCACGAAGATGAAGACAAACATAGTGATGCTGGCCTGCATGGGAACAAAGAACATCGCGAGGCTAATCACTGCCAGCAGGGCGTTCGTCCACCAGAAGATAGTGACTTTACATTTCCAGTCGGTCAGAGGTTTTGCCAGTGCGGAACCAATCAGGTTACCCACGC  >  NC_000913/3834847‑3835112                                                                                                                                      |                                                                                                                                     gATCCCGGTCAGGCGTTTACCATTGCACCACTCGCCGTAGTCGACGGTATCGGACATCATTACCCACTGGATAGGTGTCACCAGTTGATGCAACACACCAATCACGAAGATGAAGACAAACATAGTGATGCTGTCCTGCa                                                                                                                                <  2:5851/140‑1 (MQ=255)        gTCAGGCGTTTACCATTGCACCACTCGCCGTAGTCGACGGTATCGGACATCATTACCCACTGGATAGGTGTCACCAGTTGATGCAACACACCAATCACGAAGATGAAGACAAACATAGTGATGCTGTCCTGCa                                                                                                                                <  1:288726/133‑1 (MQ=255)        gTCAGGCGTTTACCATTGCACCACTCGCCGTAGTCGACGGTATCGGACATCATTACCCACTGGATAGGTGTCACCAGTTGATGCAACACACCAATCACGAAGATGAAGACAAACATAGTGATGCTGTCCTGCa                                                                                                                                >  2:288726/1‑133 (MQ=255)            ggCGTTTACCATTGCACCACTCGCCGTAGTCGACGGTATCGGACATCATTACCCACTGGATAGGTGTCACCAGTTGATGCAACACACCAATCACGAAGATGAAGACAAACATAGTGATGCTGTCCTGCATGGGAACAAAg                                                                                                                     >  2:317853/1‑140 (MQ=255)                                           gACGGTATCGGACATCATTACCCACTGGATAGGTGTCACCAGTTGATGCAACACACCAATCACGAAGATGAAGACAAACATAGTGATGCTGTCCTGCATGGGAACAAAGAACATCGCGAGGCTAATCACTGCCAGCAggg                                                                                      <  2:334297/140‑1 (MQ=255)                                                          catTACCCACTGGATAGGTGTCACCAGTTGATGCAACACACCAATCACGAAGATGAAGACAAACATAGTGATGCTGTCCTGCATGGGAACAAAGAACATCGCGAGGCTAATCACTGCCAGCAGGGCGTTCGTCCACCaga                                                                       >  1:136441/1‑140 (MQ=255)                                                          catTACCCACTGGATAGGTGTCACCAGTTGATGCAACACACCAATCACGAAGATGAAGACAAACATAGTGATGCTGTCCTGCATGGGAACAAAGAACATCGCGAGGCTAATCACTGCCAGCAGGGCGTTCGTCCACCaga                                                                       >  2:214875/1‑140 (MQ=255)                                                                    tGGATAGGTGTCACCAGTTGATGCAACACACCAATCACGAAGATGAAGACAAACATAGTGATGCTGTCCTGCATGGGAACAAAGAACATCGCGAGGCTAATCACTGCCAGCAGGGCGTTCGTc                                                                              >  1:257288/1‑123 (MQ=255)                                                                    tGGATAGGTGTCACCAGTTGATGCAACACACCAATCACGAAGATGAAGACAAACATAGTGATGCTGTCCTGCATGGGAACAAAGAACATCGCGAGGCTAATCACTGCCAGCAGGGCGTTCGTc                                                                              <  2:257288/123‑1 (MQ=255)                                                                    tGGATAGGTGTCACCAGTTGATGCAACACACCAATCACGAAGATGAAGACAAACATAGTGATGCTGTCCTGCATGGGAACAAAGAACATCGCGAGGCTAATCACTGCCAGCAGGGCGTTCGTCCACCag                                                                        >  2:177521/1‑129 (MQ=255)                                                                    tGGATAGGTGTCACCAGTTGATGCAACACACCAATCACGAAGATGAAGACAAACATAGTGATGCTGTCCTGCATGGGAACAAAGAACATCGCGAGGCTAATCACTGCCAGCAGGGCGTTCGTCCACCag                                                                        <  1:177521/129‑1 (MQ=255)                                                                     ggATAGGTGTCACCAGTTGATGCAACACACCAATCACGAAGATGAAGACAAACATAGTGATGCTGTCCTGCATGGGAACAAAGAACATCGCGAGGCTAATCACTGCCAGCAGGGCGTTCGTCCACCAGAAGATAGTGACt                                                            >  1:347995/1‑140 (MQ=255)                                                                           gtgtCACCAGTTGATGCAACACACCAATCACGAAGATGAAGACAAACATAGTGATGCTGTCCTGCATGGGAACAAAGAACATCGCGAGGCTAATCACTGCCAGCAGGGCGTTCGTCCACCAGAAGATAGTGACTTTACAt                                                      >  1:77600/1‑140 (MQ=255)                                                                           gtgtCACCAGTTGATGCAACACACCAATCACGAAGATGAAGACAAACATAGTGATGCTGTCCTGCATGGGAACAAAGAACATCGCGAGGCTAATCACTGCCAGCAGGGCGTTCGTCCACCAGAAGATAGTGACTTTACAt                                                      >  2:203351/1‑140 (MQ=255)                                                                              tCACCAGTTGATGCAACACACCAATCACGAAGATGAAGACAAACATAGTGATGCTGTCCTGCATGGGAACAAAGAACATCGCGAGGCTAATCACTGCCAGCAGGGCGTTCGTCCACCAGAAGATAGTGACTTTACATTTc                                                   >  1:47481/1‑140 (MQ=255)                                                                                aCCAGTTGATGCAACACACCAATCACGAAGATGAAGACAAACATAGTGATGCTGTCCTGCATGGGAACAAAGAACATCGCGAGGCTAATCACTGCCAGCAGGGCGTTCGTCCACCAGAAGATAGTGACTTTACATTTCCa                                                 >  1:107346/1‑140 (MQ=255)                                                                                aCCAGTTGATGCAACACACCAATCACGAAGATGAAGACAAACATAGTGATGCTGTCCTGCATGGGAACAAAGAACATCGCGAGGCTAATCACTGCCAGCAGGGCGTTCGTCCACCAGAAGATAGTGACTTTACATTTCCa                                                 >  2:287695/1‑140 (MQ=255)                                                                                  cAGTTGATGCAACACACCAATCACGAAGATGAAGACAAACATAGTGATGCTGTCCTGCATGGGAACAAAGAACATCGCGAGGCTAATCACTGCCAGCAGGGCGTTCGTCCACCAGAAGATAGTGACTTTACATTTCCAGt                                               <  1:154195/140‑1 (MQ=255)                                                                                        aTGCAACACACCAATCACGAAGATGAAGACAAACATAGTGATGCTGTCCTGCATGGGAACAAAGAACATCGCGAGGCTAATCACTGCCAGCAGGGCGTTCGTCCACCAGAAGATAGTGACTTTACATTTCCAGTCGGTCa                                         <  2:77600/140‑1 (MQ=255)                                                                                                  ccAATCACGAAGATGAAGACAAACATAGTGATGCTGTCCTGCATGGGAACAAAGAACATCGCGAGGCTAATCACTGCCAGCAGGGCGTTCGTCCACCAGAAGATAGTGACTTTACATTTCCAGTCGGTCAGAGGTTTTGc                               <  2:132719/140‑1 (MQ=255)                                                                                                                  aGACAAACATAGTGATGCTGTCCTGCATGGGAACAAAGAACATCGCGAGGCTAATCACTGCCAGCAGGGCGTTCGTCCACCAGAAGATAGTGACTTTACATTTCCAGTCGGTCAGAGGTTTTGCCAGTGCGGAACCAATc               >  1:401755/1‑140 (MQ=255)                                                                                                                  aGACAAACATAGTGATGCTGTCCTGCATGGGAACAAAGAACATCGCGAGGCTAATCACTGCCAGCAGGGCGTTCGTCCACCAGAAGATAGTGACTTTACATTTCCAGTCGGTCAGAGGTTTTGCCAGTGCGGAACCAATc               >  1:44412/1‑140 (MQ=255)                                                                                                                    aCAAACATAGTGATGCTGTCCTGCATGGGAACAAAGAACATCGCGAGGCTAATCACTGCCAGCAGGGCGTTCGTCCACCAGAAGATAGTGACTTTACATTTCCAGTCGGTCAGAGGTTTTGCCAGTGCGGAACCAATCAg             <  2:401755/140‑1 (MQ=255)                                                                                                                      aaaCATAGTGATGCTGTCCTGCATGGGAACAAAGAACATCGCGAGGCTAATCACTGCCAGCAGGGCGTTCGTCCACCAGAAGATAGTGACTTTACATTTCCAGTCGGTCAGAGGTTTTGCCAGTGCGGAACCAATCAGGt           >  2:206018/1‑140 (MQ=255)                                                                                                                          aTAGTGATGCTGTCCTGCATGGGAACAAAGAACATCGCGAGGCTAATCACTGCCAGCAGGGCGTTCGTCCACCAGAAGATAGTGACTTTACATTTCCAGTCGGTCAGAGGTTTTGCCAGTGCGGAACCAATCAGGTTAcc       <  2:136441/140‑1 (MQ=255)                                                                                                                             gTGATGCTGTCCTGCATGGGAACAAAGAACATCGCGAGGCTAATCACTGCCAGCAGGGCGTTCGTCCACCAGAAGATAGTGACTTTACATTTCCAGTCGGTCAGAGGTTTTGCCAGTGCGGAACCAATCAGGTTACCCAc    >  2:349518/1‑140 (MQ=255)                                                                                                                               gATGCTGTCCTGCATGGGAACAAAGAACATCGCGAGGCTAATCACTGCCAGCAGGGCGTTCGTCCACCAGAAGATAGTGACTTTACATTTCCAGTCGGTCAGAGGTTTTGCCAGTGCGGAACCAATCAGGTTACCCACGc  <  2:347995/140‑1 (MQ=255)                                                                                                                                      |                                                                                                                                     GATCCCGGTCAGGCGTTTACCATTGCACCACTCGCCGTAGTCGACGGTATCGGACATCATTACCCACTGGATAGGTGTCACCAGTTGATGCAACACACCAATCACGAAGATGAAGACAAACATAGTGATGCTGGCCTGCATGGGAACAAAGAACATCGCGAGGCTAATCACTGCCAGCAGGGCGTTCGTCCACCAGAAGATAGTGACTTTACATTTCCAGTCGGTCAGAGGTTTTGCCAGTGCGGAACCAATCAGGTTACCCACGC  >  NC_000913/3834847‑3835112

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑