Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,135,618	T→G	L595R (CTG→CGG)	katG →	hydroperoxidase I

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,135,618	0	T	G	100.0%	62.6 / NA	18	L595R (CTG→CGG)	katG	hydroperoxidase I
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (8/10);  total (8/10)

GTTTGCGCCGGGTCGCGTTGATGCGCGTCAGGATCAGACTGACATTGAGATGTTTGAGCTGCTGGAGCCAATTGCTGACGGTTTCCGTAACTATCGCGCTCGTCTGGACGTTTCCACCACCGAGTCACTGCTGATCGACAAAGCACAGCAACTGACGCTGACCGCGCCGGAAATGACTGCGCTGGTGGGCGGCATGCGTGTACTGGGTGCCAACTTCGATGGCAGCAAAAACGGCGTCTTCACTGACCGCGTTGGCGTATTGA  >  NC_000913/4135487‑4135749                                                                                                                                    |                                                                                                                                    gTTTGCGCCGGGTCGCGTTGATGCGCGTCAGGATCAGACTGACATTGAGATGTTTGAGCTGCTGGAGCCAATTGCTGACGGTTTCCGTAACTATCGCGCTCGTCTGGACGTTTCCACCACCGAGTCACTGCGGATCGACa                                                                                                                             <  1:141671/140‑1 (MQ=255)  tttGCGCCGGGTCGCGTTGATGCGCGTCAGGATCAGACTGACATTGAGATGTTTGAGCTGCTGGAGCCAATTGCTGACGGTTTCCGTAACTATCGCGCTCGTCTGGACGTTTCCACCACCGAGTCACTGCGGATCGACaa                                                                                                                            >  2:57887/1‑140 (MQ=255)  tttGCGCCGGGTCGCGTTGATGCGCGTCAGGATCAGACTGACATTGAGATGTTTGAGCTGCTGGAGCCAATTGCTGACGGTTTCCGTAACTATCGCGCTCGTCTGGACGTTTCCACCACCGAGTCACTGCGGATCGACaa                                                                                                                            >  2:386580/1‑140 (MQ=255)  tttGCGCCGGGTCGCGTTGATGCGCGTCAGGATCAGACTGACATTGAGATGTTTGAGCTGCTGGAGCCAATTGCTGACGGTTTCCGTAACTATCGCGCTCGTCTGGACGTTTCCACCACCGAGTCACTGCGGATCGACaa                                                                                                                            >  2:280445/1‑140 (MQ=255)                                     gACTGACATTGAGATGTTTGAGCTGCTGGAGCCAATTGCTGACGGTTTCCGTAACTATCGCGCTCGTCTGGACGTTTCCACCACCGAGTCACTGCGGATCGACAAAGCACAGCAACTGACGCTGACcgcg                                                                                                   >  1:238298/1‑130 (MQ=255)                                     gACTGACATTGAGATGTTTGAGCTGCTGGAGCCAATTGCTGACGGTTTCCGTAACTATCGCGCTCGTCTGGACGTTTCCACCACCGAGTCACTGCGGATCGACAAAGCACAGCAACTGACGCTGACcgcg                                                                                                   <  2:238298/130‑1 (MQ=255)                                            aTTGAGATGTTTGAGCTGCTGGAGCCAATTGCTGACGGTTTCCGTAACTATCGCGCTCGTCTGGACGTTTCCACCACCGAGTCACTGCGGATCGACAAAGCACAGCAACTGACGCTGACCGCGCCGGAAATGACTGCGCt                                                                                  >  2:30/1‑140 (MQ=255)                                                     tttGAGCTGCTGGAGCCAATTGCTGACGGTTTCCGTAACTATCGCGCTCGTCTGGACGTTTCCACCACCGAGTCACTGCGGATCGACAAAGCACAGCAACTGACGCTGACCGCGCCGGAAATGACTGCGCTGGTGggcgg                                                                         <  1:280445/140‑1 (MQ=255)                                                     tttGAGCTGCTGGAGCCAATTGCTGACGGTTTCCGTAACTATCGCGCTCGTCTGGACGTTTCCACCACCGAGTCACTGCGGATCGACAAAGCACAGCAACTGACGCTGACCGCGCCGGAAATGACTGCGCTGGTGggcgg                                                                         <  1:75103/140‑1 (MQ=255)                                                              ctgGAGCCAATTGCTGACGGTTTCCGTAACTATCGCGCTCGTCTGGACGTTTCCACCACCGAGTCACTGCGGATCGACAAAGCACAGCAACTGACGCTGACCGCGCCGGAAATGACTGCGCTGGTGGGCGGCa                                                                       <  2:148585/133‑1 (MQ=255)                                                              ctgGAGCCAATTGCTGACGGTTTCCGTAACTATCGCGCTCGTCTGGACGTTTCCACCACCGAGTCACTGCGGATCGACAAAGCACAGCAACTGACGCTGACCGCGCCGGAAATGACTGCGCTGGTGGGCGGCa                                                                       >  1:148585/1‑133 (MQ=255)                                                              ctgGAGCCAATTGCTGACGGTTTCCGTAACTATCGCGCTCGTCTGGACGTTTCCACCACCGAGTCACTGCGGATCGACAAAGCACAGCAACTGACGCTGACCGCGCCGGAAATGACTGCGCTGGTGGGCGGCATGCgtgt                                                                >  2:145260/1‑140 (MQ=255)                                                                                                         tGGACGTTTCCACCACCGAGTCACTGCGGATCGACAAAGCACAGCAACTGACGCTGACCGCGCCGGAAATGACTGCGCTGGTGGGCGGCATGCGTGTACTGGGTGCCAACTTCGATGGCAGCAAAAACGGCGTCTTCACt                     <  1:30/140‑1 (MQ=255)                                                                                                                ttCCACCACCGAGTCACTGCGGATCGACAAAGCACAGCAACTGACGCTGACCGCGCCGGAAATGACTGCGCTGGTGGGCGGCATGCGTGTACTGGGTGCCAACTTCGATGGCAGCAAAAACGGCGTCTTCACTGACcgcg              >  2:114844/1‑140 (MQ=255)                                                                                                                ttCCACCACCGAGTCACTGCGGATCGACAAAGCACAGCAACTGACGCTGACCGCGCCGGAAATGACTGCGCTGGTGGGCGGCATGCGTGTACTGGGTGCCAACTTCGATGGCAGCAAAAACGGCGTCTTCACTGACcgcg              <  1:114844/140‑1 (MQ=255)                                                                                                                  ccaccaCCGAGTCACTGCGGATCGACAAAGCACAGCAACTGACGCTGACCGCGCCGGAAATGACTGCGCTGGTGGGCGGCATGCGTGTACTGGGTGCCAACTTCGATGGCAGCAAAAACGGCGTCTTCACTGACCGCGtt            <  1:381503/140‑1 (MQ=255)                                                                                                                   caccacCGAGTCACTGCGGATCGACAAAGCACAGCAACTGACGCTGACCGCGCCGGAAATGACTGCGCTGGTGGGCGGCATGCGTGTACTGGGTGCCAACTTCGATGGCAGCAAAAACGGCGTCTTCACTGACCGCGTTg           <  1:162105/140‑1 (MQ=255)                                                                                                                            gTCACTGCGGATCGACAAAGCACAGCAACTGACGCTGACCGCGCCGGAAATGACTGCGCTGGTGGGCGGCATGCGTGTACTGGGTGCCAACTTCGATGGCAGCAAAAACGGCGTCTTCACTGACCGCGTTGGCGTATTGa  <  1:386580/140‑1 (MQ=255)                                                                                                                                    |                                                                                                                                    GTTTGCGCCGGGTCGCGTTGATGCGCGTCAGGATCAGACTGACATTGAGATGTTTGAGCTGCTGGAGCCAATTGCTGACGGTTTCCGTAACTATCGCGCTCGTCTGGACGTTTCCACCACCGAGTCACTGCTGATCGACAAAGCACAGCAACTGACGCTGACCGCGCCGGAAATGACTGCGCTGGTGGGCGGCATGCGTGTACTGGGTGCCAACTTCGATGGCAGCAAAAACGGCGTCTTCACTGACCGCGTTGGCGTATTGA  >  NC_000913/4135487‑4135749

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑