Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,147,953	C→A	G122V (GGT→GTT)	yijO ←	putative DNA‑binding transcriptional regulator YijO

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,147,953	0	C	A	100.0%	49.4 / NA	16	G122V (GGT→GTT)	yijO	putative DNA‑binding transcriptional regulator YijO
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (6/10);  total (6/10)

CTGGATTTGGCTGCCAAGCAAATCGCGGCAGTGGCTAAGCAGGCTGGCGACGATAAGCCTTGCCGTTTGCTGCTCCTGCGGCTGCATTTGCATTTCATTGAGCGTTTGTAGCAGAAAAGAACCTATGCGTGGGCCGCGTCGGGCGACGTGTTGCTTCGCCAGATTTTGATATTGTTTGCCATCCCATTGCACGACGCTGAAGCCGAGTTGCTGTTTGCCAAACAGCACGCTAAAGGTAGTAGCGGGG  >  NC_000913/4147832‑4148078                                                                                                                          |                                                                                                                              cTGGATTTGGCTGCCAAGCAAATCGCGGCAGTGGCTAAGCAGGCTGGCGACGATAAGCCTTGCCGTTTGCTGCTCCTGCGGCTGCATTTGCATTTCATTGAGCGTTTGTAGCAGAAAAGAAACTATGCGTGGGCCGCGTc                                                                                                             <  2:353143/140‑1 (MQ=255)          gCTGCCAAGCAAATCGCGGCAGTGGCTAAGCAGGCTGGCGACGATAAGCCTTGCCGTTTGCTGCTCCTGCGGCTGCATTTGCATTTCATTGAGCGTTTGTAGCAGAAAAGAAACTATGCGTGGGCCGCGTCGGGCGACgg                                                                                                    >  1:189299/1‑139 (MQ=255)                aaGCAAATCGCGGCAGTGGCTAAGCAGGCTGGCGACGATAAGCCTTGCCGTTTGCTGCTCCTGCGGCTGCATTTGCATTTCATTGAGCGTTTGTAGCAGAAAAGAAACTATGCTTGGGCCGCGTCGGGCGACGTGTTGCt                                                                                              <  1:102210/140‑1 (MQ=255)                                tGGCTAAGCAGGCTGGCGACGATAAGCCTTGCCGTTTGCTGCTCCTGCGGCTGCATTTGCATTTCATTGAGCGTTTGTAGCAGAAAAGAAACTATGCGTGGGCCGCGTCGGGCGACGTGTTGCTTCGCCAGATTTTg                                                                                 >  1:190400/1‑137 (MQ=255)                                tGGCTAAGCAGGCTGGCGACGATAAGCCTTGCCGTTTGCTGCTCCTGCGGCTGCATTTGCATTTCATTGAGCGTTTGTAGCAGAAAAGAAACTATGCGTGGGCCGCGTCGGGCGACGTGTTGCTTCGCCAGATTTTg                                                                                 <  2:190400/137‑1 (MQ=255)                                             tGGCGACGATAAGCCTTGCCGTTTGCTGCTCCTGCGGCTGCATTTGCATTTCATTGAGCGTTTGTAGCAGAAAAGAAACTATGCGTGGGCCGCGTCGGGCGACGTGTTGCTTCGCCAGATTTTGATATTGTTTGCCATcc                                                                 <  1:269771/140‑1 (MQ=255)                                                                        gctCCTGCGGCTGCATTTGCATTTCATTGAGCGTTTGTAGCAGAAAAGAAACTATGCGTGGGCCGCGTCGGGCGACGTGTTGCTTCGCCAGATTTTGATATTGTTTGCCATCCCATTGCACGACGCTGAAGCCGAGTTGc                                      >  2:353123/1‑140 (MQ=255)                                                                                 gCTGCATTTGCATTTCATTGAGCGTTTGTAGCAGAAAAGAAACTATGCGTGGGCCGCGTCGGGCGACGTGTTGCTTCGCCAGATTTTGATATTGTTTGCCATCCCATTGCACGACGCTGAAGCCGAGTTGCTGTTTGCCa                             >  1:68883/1‑140 (MQ=255)                                                                                      atttgcatttCATTGAGCGTTTGTAGCAGAAAAGAAACTATGCGTGGGCCGCGTCGGGCGACGTGTTGCTTCGCCAGATTTTGATATTGTTTGCCATCCCATTGCACGACGCTGAAGCCGAGTTGCTGTTTGCCAAACAg                        <  2:377189/140‑1 (MQ=255)                                                                                          gcatttCATTGAGCGTTTGTAGCAGAAAAGAAACTATGCGTGGGCCGCGTCGGGCGACGTGTTGCTTCGCCAGATTTTGATATTGTTTGCCATCCCATTGCACGACGCTGAAGCCGAGTTGCTGTTTGCCAAACAGCACg                    <  2:189299/140‑1 (MQ=255)                                                                                            atttCATTGAGCGTTTGTAGCAGAAAAGAAACTATGCGTGGGCCGCGTCGGGCGACGTGTTGCTTCGCCAGATTTTGATATTGTTTGCCATCCCATTGCACGACGCTGAAGCCGAGTTGCTGTTTGCCAAACAGCACGCt                  <  2:68883/140‑1 (MQ=255)                                                                                              ttCATTGAGCGTTTGTAGCAGAAAAGAAACTATGCGTGGGCCGCGTCGGGCGACGTGTTGCTTCGCCAGATTTTGATATTGTTTGCCATCCCATTGCACGACGCTGAAGCCGAGTTGCTGTTTGCCAAACAGCACGCTaa                >  1:275115/1‑140 (MQ=255)                                                                                               tCATTGAGCGTTTGTAGCAGGAAAGAAACTATGCGTGGGCCGCGTCGGGCGACGTGTTGCTTCGCCAGATTTTGATATTGTTTGCCATCCCATTGCACGACGCTGAAGCCGAGTTGCTGTTTGCCAAACAg                        >  1:92208/1‑131 (MQ=255)                                                                                               tCATTGAGCGTTTGTAGCAGGAAAGAAACTATGCGTGGGCCGCGTCGGGCGACGTGTTGCTTCGCCAGATTTTGATATTGTTTGCCATCCCATTGCACGACGCTGAAGCCGAGTTGCTGTTTGCCAAACAg                        <  2:92208/131‑1 (MQ=255)                                                                                                          ttGTAGCAGAAAAGAAACTATGCGTGGGCCGCGTCGGGCGACGTGTTGCTTCGCCAGATTTTGATATTGTTTGCCATCCCATTGCACGACGCTGAAGCCGAGTTGCTGTTTGCCAAACAGCACGCTAAAGGTAGTAGCgg    <  1:284004/140‑1 (MQ=255)                                                                                                            gTAGCAGAAAAGAAACTATGCGTGGGCCGCGTCGGGCGACGTGTTGCTTCGCCAGATTTTGATATTGTTTGCCATCCCATTGCACGACGCTGAAGCCGAGTTGCTGTTTGCCAAACAGCACGCTAAAGGTAGTAGCgggg  <  2:275115/140‑1 (MQ=255)                                                                                                                          |                                                                                                                              CTGGATTTGGCTGCCAAGCAAATCGCGGCAGTGGCTAAGCAGGCTGGCGACGATAAGCCTTGCCGTTTGCTGCTCCTGCGGCTGCATTTGCATTTCATTGAGCGTTTGTAGCAGAAAAGAACCTATGCGTGGGCCGCGTCGGGCGACGTGTTGCTTCGCCAGATTTTGATATTGTTTGCCATCCCATTGCACGACGCTGAAGCCGAGTTGCTGTTTGCCAAACAGCACGCTAAAGGTAGTAGCGGGG  >  NC_000913/4147832‑4148078

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑