Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,529,836	G→T	T136T (ACC→ACA)	sgcC ←	putative PTS enzyme IIC component SgcC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,529,836	0	G	T	100.0%	108.1 / NA	31	T136T (ACC→ACA)	sgcC	putative PTS enzyme IIC component SgcC
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (17/14);  total (17/14)

GAACCGTAGCCTTGCGGTATCGAAATACCTTCCAGCCCGACGATGTTCTGTACCCGTTTTGCCGTCCAGTCGGCCATTTTTAACGATAGCGCCGCATGGCAGATTGCCCCCAGCACACCATAAATCAGGCTACCGGTCATAAGCTGGACCACCGTACCCGTAATGGCGTAGTGCCAGTAATTGTAAATATCGACGTTCATCGTTTTGGTCAGACGGGTGACCAGCATGGCGACATTGAGAAGAAAAATAACTGGGATAATCATGGCGC  >  NC_000913/4529702‑4529969                                                                                                                                       |                                                                                                                                      gAACCGTAGCCTTGCGGTATCGAAATACCTTCCAGCCCGACGATGTTCTGTACCCGTTTTGCCGTCCAGTCGGCCATTTTTAACGATAGCGCCGCATGGCAGATTGCCCCCAGCACACCATAAATCAGGCTACCTGTCAt                                                                                                                                  >  2:54768/1‑140 (MQ=255)   aCCGTAGCCTTGCGGTATCGAAATACCTTCCAGCCCGACGATGTTCTGTACCCGTTTTGCCGTCCAGTCGGCCATTTTTAACGATAGCGCCGCATGGCAGATTGCCCCCAGCACACCATAAATCAGGCTACCTGTCATaa                                                                                                                                >  1:318049/1‑140 (MQ=255)      gTAGCCTTGCGGTATCGAAATACCTTCCAGCCCGACGATGTTCTGTACCCGTTTTGCCGTCCAGTCGGCCATTTTTAACGATAGCGCCGCATGGCAGATTGCCCCCAGCACACCATAAATCAGGCTACCTGTCATAAGCt                                                                                                                             >  2:114545/1‑140 (MQ=255)       tAGCCTTGCGGTATCGAAATACCTTCCAGCCCGACGATGTTCTGTACCCGTTTTGCCGTCCAGTCGGCCATTTTTAACGATAGCGCCGCATGGCAGATTGCCCCCAGCACACCATAAATCAGGCTACCTGTCATAAGCTg                                                                                                                            >  2:116/1‑140 (MQ=255)              gCGGTATCGAAATACCTTCCAGCCCGACGATGTTCTGTACCCGTTTTGCCGTCCAGTCGGCCATTTTTAACGATAGCGCCGCATGGCAGATTGCCCCCAGCACACCATAAATCAGGCTACCTGTCATAAGCTGGaccacc                                                                                                                     <  1:116/140‑1 (MQ=255)                    tCGAAATACCTTCCAGCCCGACGATGTTCTGTACCCGTTTTGCCGTCCAGTCGGCCATTTTTAACGATAGCGCCGCATGGCAGATTGCCCCCAGCACACCATAAATCAGGCTACCTGTCATAAGCTGGACCACCGt                                                                                                                   >  1:303548/1‑136 (MQ=255)                    tCGAAATACCTTCCAGCCCGACGATGTTCTGTACCCGTTTTGCCGTCCAGTCGGCCATTTTTAACGATAGCGCCGCATGGCAGATTGCCCCCAGCACACCATAAATCAGGCTACCTGTCATAAGCTGGACCACCGt                                                                                                                   <  2:303548/136‑1 (MQ=255)                             cTTCCAGCCCGACGATGTTCTGTACCCGTTTTGCCGTCCAGTCGGCCATTTTTAACGATAGCGCCGCATGGCAGATTGCCCCCAGCACACCATAAATCAGGCTACCTGTCATAAGCTGGACCACCGTACCCGTAATGGCg                                                                                                      <  2:310654/140‑1 (MQ=255)                             cTTCCAGCCCGACGATGTTCTGTACCCGTTTTGCCGTCCAGTCGGCCATTTTTAACGATAGCGCCGCATGGCAGATTGCCCCCAGCACACCATAAATCAGGCTACCTGTCATAAGCTGGACCACCGTACCCGTAATGGCg                                                                                                      <  1:54768/140‑1 (MQ=255)                              ttCCAGCCCGACGATGTTCTGTACCCGTTTTGCCGTCCAGTCGGCCATTTTTAACGATAGCGCCGCATGGCAGATTGCCCCCAGCACACCATAAATCAGGCTACCTGTCATAAGCTGGACCACCGTACCCGTAATGGCGt                                                                                                     <  2:333567/140‑1 (MQ=255)                               tCCAGCCCGACGATGTTCTGTACCCGTTTTGCCGTCCAGTCGGCCATTTTTAACGATAGCGCCGCATGGCAGATTGCCCCCAGCACACCATAAATCAGGCTACCTGTCATAAGCTGGACCACCGTACCCGTAATGGCGTa                                                                                                    >  1:262170/1‑140 (MQ=255)                                 cAGCCCGACGATGTTCTGTACCCGTTTTGCCGTCCAGTCGGCCATTTTTAACGATAGCGCCGCATGGCAGATTGCCCCCAGCACACCATAAATCAGGCTACCTGTCATAAGCTGGACCACCGTACCCGTAATGGCGTAGt                                                                                                  <  1:57773/140‑1 (MQ=255)                                 cAGCCCGACGATGTTCTGTACCCGTTTTGCCGTCCAGTCGGCCATTTTTAACGATAGCGCCGCATGGCAGATTGCCCCCAGCACACCATAAATCAGGCTACCTGTCATAAGCTGGACCACCGTACCCGTAATGGCGTAGt                                                                                                  >  2:287945/1‑140 (MQ=255)                                      cgacgaTGTTCTGTACCCGTTTTGCCGTCCAGTCGGCCATTTTTAACGATAGCGCCGCATGGCAGATTGCCCCCAGCACACCATAAATCAGGCTACCTGTCATAAGCTGGACCACCGTACCCGTAATGGCGTAGTGCCAg                                                                                             <  2:249552/140‑1 (MQ=255)                                      cgacgaTGTTCTGTACCCGTTTTGCCGTCCAGTCGGCCATTTTTAACGATAGCGCCGCATGGCAGATTGCCCCCAGCACACCATAAATCAGGCTACCTGTCATAAGCTGGACCACCGTACCCGTAATGGCGTAGTGCCAg                                                                                             <  2:262170/140‑1 (MQ=255)                                       gacgaTGTTCTGTACCCGTTTTGCCGTCCAGTCGGCCATTTTTAACGATAGCGCCGCATGGCAGATTGCCCCCAGCACACCATAAATCAGGCTACCTGTCATAAGCTGGACCACCGTACCCGTAATGGCGTAGTGCCAGt                                                                                            <  2:237781/140‑1 (MQ=255)                                                cTGTACCCGTTTTGCCGTCCAGTCGGCCATTTTTAACGATAGCGCCGCATGGCAGATTGCCCCCAGCACACCATAAATCAGGCTACCTGTCATAAGCTGGACCACCGTACCCGTAATGGCGTAGTGCCAGTAATTGTaaa                                                                                   >  2:316186/1‑140 (MQ=255)                                                                  ccAGTCGGCCATTTTTAACGATAGCGCCGCATGGCAGATTGCCCCCAGCACACCATAAATCAGGCTACCTGTCATAAGCTGGACCACCGTACCCGTAATGGCGTAGTGCCAGTAATTGTAAATATCGACGTTCATCGttt                                                                 >  1:107988/1‑140 (MQ=255)                                                                          ccATTTTTAACGATAGCGCCGCATGGCAGATTGCCCCCAGCACACCATAAATCAGGCTACCTGTCATAAGCTGGACCACCGTACCCGTAATGGCGTAGTGCCAGTAATTGTAAATATCGACGTTCATCGTTTTGGTCAGa                                                         <  1:287945/140‑1 (MQ=255)                                                                             tttttAACGATAGCGCCGCATGGCAGATTGCCCCCAGCACACCATAAATCAGGCTACCTGTCATAAGCTGGACCACCGTACCCGTAATGGCGTAGTGCCAGTAATTGTAAATATCGACGTTCATCGTTTTGGTCAGACgg                                                      <  2:107988/140‑1 (MQ=255)                                                                             tttttAACGATAGCGCCGCATGGCAGATTGCCCCCAGCACACCATAAATCAGGCTACCTGTCATAAGCTGGACCACCGTACCCGTAATGGCGTAGTGCCAGTAATTGTAAATATCGACGTTCATCGTTTTGGTCAGACgg                                                      <  1:108263/140‑1 (MQ=255)                                                                               tttAACGATAGCGCCGCATGGCAGATTGCCCCCAGCACACCATAAATCAGGCTACCTGTCATAAGCTGGACCACCGTACCCGTAATGGCGTAGTGCCAGTAATTGTAAATATCGACGTTCATCGTTTTGGTCAGACGGGt                                                    >  2:341346/1‑140 (MQ=255)                                                                                               cATGGCAGATTGCCCCCAGCACACCATAAATCAGGCTACCTGTCATAAGCTGGACCACCGTACCCGTAATGGCGTAGTGCCAGTAATTGTAAATATCGACGTTCATCGTTTTGGTCAGACGGGTGACCAGCATGGCGACa                                    >  1:8465/1‑140 (MQ=255)                                                                                                 tGGCAGATTGCCCCCAGCACACCATAAATCAGGCTACCTGTCATAAGCTGGACCACCGTACCCGTAATGGCGTAGTGCCAGTAATTGTAAATATCGACGTTCATCGTTTTGGTCAGACGGGTGACCAGCATGGCGACAtt                                  <  2:318049/140‑1 (MQ=255)                                                                                                                 gCACACCATAAATCAGGCTACCTGTCATAAGCTGGACCACCGTACCCGTAATGGCGTAGTGCCAGTAATTGTAAATATCGACGTTCATCGTTTTGGTCAGACGGGTGACCAGCATGGCGACATTGAGAAGAAAAATAACt                  >  2:389520/1‑140 (MQ=255)                                                                                                                       cATAAATCAGGCTACCTGTCATAAGCTGGACCACCGTACCCGTAATGGCGTAGTGCCAGTAATTGTAAATATCGACGTTCATCGTTTTGGTCAGACGGGTGACCAGCATGGCGACATTGAGAAGAAAAATAACTGGGATa            >  2:16425/1‑140 (MQ=255)                                                                                                                            aTCAGGCTACCTGTCATAAGCTGGACCACCGTACCCGTAATGGCGTAGTGCCAGTAATTGTAAATATCGACGTTCATCGTTTTGGTCAGACGGGTGACCAGCATGGCGACATTGAGAAGAAAAATAACTGGGATAATCAt       >  2:191753/1‑140 (MQ=255)                                                                                                                            aTCAGGCTACCTGTCATAAGCTGGACCACCGTACCCGTAATGGCGTAGTGCCAGTAATTGTAAATATCGACGTTCATCGTTTTGGTCAGACGGGTGACCAGCATGGCGACATTGAGAAGAAAAATAACTGGGATAATCAt       >  1:229468/1‑140 (MQ=255)                                                                                                                             tCAGGCTACCTGTCATAAGCTGGACCACCGTACCCGTAATGGCGTAGTGCCAGTAATTGTAAATATCGACGTTCATCGTTTTGGTCAGACGGATGACCAGCATGGCGACATTGAGAAGAAAAATAACTGGGATAATCATg      <  1:114545/140‑1 (MQ=255)                                                                                                                                 gCTACCTGTCATAAGCTGGACCACCGTACCCGTAATGGCGTAGTGCCAGTAATTGTAAATATCGACGTTCATCGTTTTGGTCAGACGGGTGACCAGCATGGCGACATTGAGAAGAAAAATAACTGTGATAATCATGgcgc  >  1:233872/1‑140 (MQ=255)                                                                                                                                 gCTACCTGTCATAAGCTGGACCACCGTACCCGTAATGGCGTAGTGCCAGTAATTGTAAATATCGACGTTCATCGTTTTGGTCAGACGGGTGACCAGCATGGCGACATTGAGAAGAAAAATAACTGGGATAATCATGgcgc  >  2:103748/1‑140 (MQ=255)                                                                                                                                       |                                                                                                                                      GAACCGTAGCCTTGCGGTATCGAAATACCTTCCAGCCCGACGATGTTCTGTACCCGTTTTGCCGTCCAGTCGGCCATTTTTAACGATAGCGCCGCATGGCAGATTGCCCCCAGCACACCATAAATCAGGCTACCGGTCATAAGCTGGACCACCGTACCCGTAATGGCGTAGTGCCAGTAATTGTAAATATCGACGTTCATCGTTTTGGTCAGACGGGTGACCAGCATGGCGACATTGAGAAGAAAAATAACTGGGATAATCATGGCGC  >  NC_000913/4529702‑4529969

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑