Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	435,206	C→A	Q24K (CAG→AAG)	nusB →	transcription antitermination protein NusB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	435,206	0	C	A	100.0%	89.1 / NA	26	Q24K (CAG→AAG)	nusB	transcription antitermination protein NusB
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (12/14);  total (12/14)

CTTGAAATGATTAATGTATTGAAAGCCATCAAGGCCTGAAATTAGTAAGGGGAAATCCGTGAAACCTGCTGCTCGTCGCCGCGCTCGTGAGTGTGCCGTCCAGGCGCTCTACTCCTGGCAGTTGTCCCAGAACGACATCGCTGATGTTGAATACCAGTTCCTGGCTGAACAGGATGTAAAAGACGTTGACGTCCTGTACTTCCGTGAGCTGCTGGCCGGGGTGGCGACTAATACCGCATACCTCGACGGACTGATGAAGCC  >  NC_000913/435079‑435339                                                                                                                                |                                                                                                                                      cTTGAAATGATTAATGTATTGAAAGCCATCAAGGCCTGAAATTAGTAAGGGGAAATCCGTGAAACCTGCTGCTCGTCGCCGCGCTCGTGAGTGTGCCGTCCAGGCGCTCTACTCCTGGCAGTTGTCCAAGAACGACATCg                                                                                                                           >  2:344657/1‑140 (MQ=255)    gAAATGATTAATGTATTGAAAGCCATCAAGGCCTGAAATTAGTAAGGGGAAATCCGTGAAACCTGCTGCTCGTCGCCGCGCTCGTGAGTGTGCCGTCCAGGCGCTCTACTCCTGGCAGTTGTCCAAGAACGACATCGCTg                                                                                                                        <  2:201800/140‑1 (MQ=255)    gAAATGATTAATGTATTGAAAGCCATCAAGGCCTGAAATTAGTAAGGGGAAATCCGTGAAACCTGCTGCTCGTCGCCGCGCTCGTGAGTGTGCCGTCCAGGCGCTCTACTCCTGGCAGTTGTCCAAGAACGACATCGCTg                                                                                                                        <  1:405558/140‑1 (MQ=255)       aTGATTAATGTATTGAAAGCCATCAAGGCCTGAAATTAGTAAGGGGAAATCCGTGAAACCTGCTGCTCGTCGCCGCGCTCGTGAGTGTGCCGTCCAGGCGCTCTACTCCTGGCAGTTGTCCAAGAACGACATCGCTGATg                                                                                                                     <  1:139684/140‑1 (MQ=255)                  aTTGAAAGCCATCAAGGCCTGAAATTAGTAAGGGGAAATCCGTGAAACCTGCTGCTCGTCGCCGCGCTCGTGAGTGTGCCGTCCAGGCGCTCTACTCCTGGCAGTTGTCCAAGAACGACATCGCTGATGTTGAATACCAg                                                                                                          >  2:370077/1‑140 (MQ=255)                         gCCATCAAGGCCTGAAATTAGTAAGGGGAAATCCGTGAAACCTGCTGCTCGTCGCCGCGCTCGTGAGTGTGCCGTCCAGGCGCTCTACTCCTGGCAGTTGTCCAAGAACGACATCGCTGATGTTGAATACCAGTTCCTgg                                                                                                   >  1:343489/1‑140 (MQ=255)                         gCCATCAAGGCCTGAAATTAGTAAGGGGAAATCCGTGAAACCTGCTGCTCGTCGCCGCGCTCGTGAGTGTGCCGTCCAGGCGCTCTACTCCTGGCAGTTGTCCAAGAACGACATCGCTGATGTTGAATACCAGTTCCTgg                                                                                                   <  1:331280/140‑1 (MQ=255)                           cATCAAGGCCTGAAATTAGTAAGGGGAAATCCGTGAAACCTGCTGCTCGTCGCCGCGCTCGTGAGTGTGCCGTCCAGGCGCTCTACTCCTGGCAGTTGTCCAAGAACGACATCGCTGATGTTGAATACCAGTTCCTGGCt                                                                                                 >  1:343030/1‑140 (MQ=255)                             tCAAGGCCTGAAATTAGTAAGGGGAAATCCGTGAAACCTGCTGCTCGTCGCCGCGCTCGTGAGTGTGCCGTCCAGGCGCTCTACTCCTGGCAGTTGTCCAAGAACGACATCGCTGATGTTGAATACCAGTTCCTGGCTGa                                                                                               >  2:295032/1‑140 (MQ=255)                             tCAAGGCCTGAAATTAGTAAGGGGAAATCCGTGAAACCTGCTGCTCGTCGCCGCGCTCGTGAGTGTGCCGTCCAGGCGCTCTACTCCTGGCAGTTGTCCAAGAACGACATCGCTGATGTTGAATACCAGTTCCTGGCTGa                                                                                               >  1:167293/1‑140 (MQ=255)                              cAAGGCCTGAAATTAGTAAGGGGAAATCCGTGAAACCTGCTGCTCGTCGCCGCGCTCGTGAGTGTGCCGTCCAGGCGCTCTACTCCTGGCAGTTGTCCAAGAACGACATCGCTGATGTTGAATACCAGTTCCTGGCTGaa                                                                                              <  2:343030/140‑1 (MQ=255)                                   ccTGAAATTAGTAAGGGGAAATCCGTGAAACCTGCTGCTCGTCGCCGCGCTCGTGAGTGTGCCGTCCAGGCGCTCTACTCCTGGCAGTTGTCCAAGAACGACATCGCTGATGTTGAATACCAGTTCCTGGCTGaa                                                                                              >  1:390815/1‑135 (MQ=255)                                   ccTGAAATTAGTAAGGGGAAATCCGTGAAACCTGCTGCTCGTCGCCGCGCTCGTGAGTGTGCCGTCCAGGCGCTCTACTCCTGGCAGTTGTCCAAGAACGACATCGCTGATGTTGAATACCAGTTCCTGGCTGaa                                                                                              <  2:390815/135‑1 (MQ=255)                                     tGAAATTAGTAAGGGGAAATCCGTGAAACCTGCTGCTCGTCGCCGCGCTCGTGAGTGTGCCGTCCAGGCGCTCTACTCCTGGCAGTTGTCCAAGAACGACATCGCTGATGTTGAATACCAGTTCCTGGCTGAACAGGATg                                                                                       >  2:199262/1‑140 (MQ=255)                                                 ggggAAATCCGTGAAACCTGCTGCTCGTCGCCGCGCTCGTGAGTGTGCCGTCCAGGCGCTCTACTCCTGGCAGTTGTCCAAGAACGACATCGCTGATGTTGAATACCAGTTCCTGGCTGAACAGGATGTAAAAGACGTTg                                                                           <  1:153101/140‑1 (MQ=255)                                                    gAAATCCGTGAAACCTGCTGCTCGTCGCCGCGCTCGTGAGTGTGCCGTCCAGGCGCTCTACTCCTGGCAGTTGTCCAAGAACGACATCGCTGATGTTGAATACCAGTTCCTGGCTGAACAGGATGTAAAAGACGTTGACg                                                                        <  2:260694/140‑1 (MQ=255)                                                      aaTCCGTGAAACCTGCTGCTCGTCGCCGCGCTCGTGAGTGTGCCGTCCAGGCGCTCTACTCCTGGCAGTTGTCCAAGAACGACATCGCTGATGTTGAATACCAGTTCCTGGCTGAACAGGATGTAAAAGACGTTGACGTc                                                                      <  2:167293/140‑1 (MQ=255)                                                             gAAACCTGCTGCTCGTCGCCGCGCTCGTGAGTGTGCCGTCCAGGCGCTCTACTCCTGGCAGTTGTCCAAGAACGACATCGCTGATGTTGAATACCAGTTCCTGGCTGAACAGGATGTAAAAGACGTTGACGTCCTGTACt                                                               <  2:235614/140‑1 (MQ=255)                                                                   tgctgcTCGTCGCCGCGCTCGTGAGTGTGCCGTCCAGGCGCTCTACTCCTGGCAGTTGTCCAAGAACGACATCGCTGATGTTGAATACCAGTTCCTGGCTGAACAGGATGTAAAAGACGTTGACGTCCTGTACTTCCGTg                                                         >  1:142625/1‑140 (MQ=255)                                                                     ctgctCGTCGCCGCGCTCGTGAGTGTGCCGTCCAGGCGCTCTACTCCTGGCAGTTGTCCAAGAACGACATCGCTGATGTTGAATACCAGTTCCTGGCTGAACAGGATGTAAAAGACGTTGACGTCCTGTACTTCCGTGAg                                                       >  1:381650/1‑140 (MQ=255)                                                                                    cTCGTGAGTGTGCCGTCCAGGCGCTCTACTCCTGGCAGTTGTCCAAGAACGACATCGCTGATGTTGAATACCAGTTCCTGGCTGAACAGGATGTAAAAGACGTTGACGTCCTGTACTTCCGTGAGCTGCTGGCCGGGGTg                                        <  1:295032/140‑1 (MQ=255)                                                                                     tCGTGAGTGTGCCGTCCAGGCGCTCTACTCCTGGCAGTTGTCCAAGAACGACATCGCTGATGTTGAATACCAGTTCCTGGCTGAACAGGATGTAAAAGACGTTGACGTCCTGTACTTCCGTGAGCTGCTGGCCGGGGTgg                                       <  2:322540/140‑1 (MQ=255)                                                                                               gCCGTCCAGGCGCTCTACTCCTGGCAGTTGTCCAAGAACGACATCGCTGATGTTGAATACCAGTTCCTGGCTGAACAGGATGTAAAAGACGTTGACGTCCTGTACTTCCGTGAGCTGCTGGCCGGGGTGGCGACTAATAc                             >  1:143896/1‑140 (MQ=255)                                                                                                 cGTCCAGGCGCTGTACTCCTGGCAGTTGTCCAAGAGCGACATCGCTGATGTTGAATACCAGTTCCTGGCTGAACAGGATGTAAAAGACGTTGACGTCCTGTCCTTCCGTGAGCTGCTGGCCGGGGTGGCGACTAATACCg                           <  2:281642/140‑1 (MQ=255)                                                                                                             ctACTCCTGGCAGTTGTCCAAGAACGACATCGCTGATGTTGAATACCAGTTCCTGGCTGAACAGGATGTAAAAGACGTTGACGTCCTGTACTTCCGTGAGCTGCTGGCCGGGGTGGCGACTAATACCGCATACCTCGACg               >  1:317176/1‑140 (MQ=255)                                                                                                               ccTCCTGGCAGTTGTCCAAGAACGACATCGCTGATGTTGAATCCCAGTTCCTGGCTGAACAGGATGTAAAAGACGTTGACGTCCTGTACTTCCGTGAGCTGCTGGCCGGGGTGGCGACTAATACCGCATACCTCGACGGa             <  1:344657/139‑1 (MQ=255)                                                                                                                    tGGCAGTTGTCCAAGAACGACATCGCTGATGTTGAATACCAGTTCCTGGCTGAACAGGATGTAAAAGACGTTGACGTCCTGTACTTCCGTGAGCTGCTGGCCGGGGTGGCGACTAATACCGCATACCTCGACGGACtgat        <  2:219802/140‑1 (MQ=255)                                                                                                                          ttGTCCAAGAACGACATCGCTGATGTTGAATACCAGTTCCTGGCTGAACAGGATGTAAAAGACGTTGACGTCCTGTACTTCCGTGAGCTGCTGGCCGGGGTGGCGACTAATACCGCATACCTCGACGGACTGATGAAGcc  <  2:343489/140‑1 (MQ=255)                                                                                                                                |                                                                                                                                      CTTGAAATGATTAATGTATTGAAAGCCATCAAGGCCTGAAATTAGTAAGGGGAAATCCGTGAAACCTGCTGCTCGTCGCCGCGCTCGTGAGTGTGCCGTCCAGGCGCTCTACTCCTGGCAGTTGTCCCAGAACGACATCGCTGATGTTGAATACCAGTTCCTGGCTGAACAGGATGTAAAAGACGTTGACGTCCTGTACTTCCGTGAGCTGCTGGCCGGGGTGGCGACTAATACCGCATACCTCGACGGACTGATGAAGCC  >  NC_000913/435079‑435339

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑